Rroxscaffold_6G00418580
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001456
Physical Location & Seq
Reverse (-)
40137614 .. 40139919
2306 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00418580.1

Sequence Viewer

Length: 1305 bp
ATGTTTCTTGCTAACTCAAATACTCTTCCATCTCCGACCACCGGAGCCTTTTCGAGTCAGGTTTTCGAGTGGATGAGCACTCCTAAAGAAAATAACAGCCAGCAAGGAGCGGAGAAGGAGCAGAAAATGTTCACTGATTTCTCATTTCAACCGGCAACATCATCTTCATCGTTCAGCGTTCAGCAATCTTCTTCAAGCATGGTTTCATCGGTGGAGGAATCATTGAAAAGTCAACAGCAGCAATCGTGGGGTTTCAACAACCCAAGGCAAGAAGAAAGCGTAGCAGAGAAAACAGAAGTGAAATCCGAGTTTCCGCCAACTCAGAGCTTCAATGCTGCTCCAAAGTCTGATTACACACATTGCAACACTCATTCGACTCAATACGTGAGGGAACAGAAATCAGATGATGGATACAATTGGAGAAAATATGGACAGAAGCAAGTGAAAGGTAGCGAAAATCCGCGGAGTTATTACAAGTGTACATTTCCCAATTGCCCAACAAAGAAGAAGGTGGAGAGATCATTGGATGGACAGATCACTCAAATTGTGTACAAGGGAAATCACAACCATGCTAAGCCTCAGTCGACAAGACGATCAGCCTCTCAACAACAATCGCTTCAAACAAGTTCGTATGGGATTTCTGATCAGTCTGCCTCGACAATATCCAATCCAAAAATTGAAGCTGTCACGCTGCAGGAGGACTCTAACTCAGCCTCAATGGGAGGAGAGGATGAGTTTGAACAAAATTCGCCGATAAGTAACTCAAATGGAGCTGATGATGACAATGAGCCTGAGGCCAAAAGATGGAAGGGAGAAAACGCAAATGAGACGTATGCAGCTCCTGGGAGTAGAATTGTGAAAGAACCTAGAATTGTAGTGCAGACCACAAGTGAGATTGATATTCTGGATGATGGGTATAGATGGAGGAAATATGGACAGAAAGTAGTGAAGGGAAATCCAAACCCAAGGAGCTACTACAAATGTACTTCTACTGGCTGTCCGGTGAGGAAACATGTGGAGCGAGCATCTCACGATACGAGGGCCGTGATCACAACTTATGAAGGGAAGCACAACCATGATGTTCCTGCAGCACGCGGCAGCGGTGGTTATGGTGCCAGTACTAGACCTGCTTCTGATGTCAACAGCAACTGCAATGTATCAGTGGCAGCTGTGAGGCCCTTGGCCTTGCCTAATCATTCTAACAATTTGAGCTACCTTAGCTCTCTTCAGAAGCAGCCAACATCAGAAAGCCAAGCACCTTATACTCTCAAAATGTTGCAGAACACAGGAAGTTACGGATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
WRKY

Protein Analysis

434

Amino Acids

48.07

Weight (kDa)

8.34

Isoelectric Point (pI)

57.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 135 - 190 3.2e-26 WRKY DNA -binding domain
WRKY PF03106 303 - 360 1.5e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1135
AccB1I GGYRCC 1 cut(s) 1112
AccB7I CCANNNNNTGG 1 cut(s) 804
AccBSI CCGCTC 1 cut(s) 110
AccI GTMKAC 1 cut(s) 584
AccII CGCG 2 cut(s) 463, 1095
AciI CCGC 6 cut(s) 110, 314, 461, 463, 1095, 1101
AcsI RAATTY 1 cut(s) 745
AcuI CTGAAG 1 cut(s) 1211
AfaI GTAC 4 cut(s) 481, 551, 985, 1120
AfiI CCNNNNNNNGG 2 cut(s) 41, 804
AflIII ACRYGT 1 cut(s) 1012
AgsI TTSAA 8 cut(s) 149, 195, 226, 256, 331, 620, 680, 740
AjnI CCWGG 1 cut(s) 841
AluBI AGCT 8 cut(s) 327, 683, 773, 839, 972, 1169, 1212, 1221
AluI AGCT 8 cut(s) 327, 683, 773, 839, 972, 1169, 1212, 1221
Alw21I GWGCWC 1 cut(s) 80
Alw26I GTCTC 1 cut(s) 821
AlwNI CAGNNNCTG 2 cut(s) 842, 1149
AoxI GGCC 4 cut(s) 795, 1041, 1175, 1182
ApeKI GCWGC 8 cut(s) 238, 335, 691, 836, 1088, 1098, 1166, 1234
ApoI RAATTY 1 cut(s) 745
Asp700I GAANNNNTTC 1 cut(s) 128
AspS9I GGNCC 2 cut(s) 1041, 1176
AsuHPI GGTGA 1 cut(s) 1015
AxyI CCTNAGG 1 cut(s) 792
BanI GGYRCC 1 cut(s) 1112
Bbv12I GWGCWC 1 cut(s) 80
BbvI GCAGC 8 cut(s) 250, 322, 678, 848, 1100, 1110, 1178, 1246
BccI CCATC 6 cut(s) 37, 401, 521, 798, 905, 915
BceAI ACGGC 1 cut(s) 1028
BciT130I CCWGG 1 cut(s) 843
BciVI GTATCC 2 cut(s) 404, 1292
BclI TGATCA 2 cut(s) 643, 1047
BcoDI GTCTC 1 cut(s) 821
BfaI CTAG 2 cut(s) 867, 1122
BfmI CTRYAG 2 cut(s) 692, 1086
BfuAI ACCTGC 1 cut(s) 1135
BfuI GTATCC 2 cut(s) 404, 1292
BisI GCNGC 9 cut(s) 239, 336, 692, 837, 1089, 1096, 1099, 1167, 1235
BlpI GCTNAGC 1 cut(s) 573
BlsI GCNGC 9 cut(s) 240, 337, 693, 838, 1090, 1097, 1100, 1168, 1236
BmcAI AGTACT 1 cut(s) 1120
Bme1390I CCNGG 1 cut(s) 843
BmgT120I GGNCC 2 cut(s) 1041, 1176
BmiI GGNNCC 2 cut(s) 46, 1114
BmrFI CCNGG 1 cut(s) 843
BmsI GCATC 1 cut(s) 1034
Bpu10I CCTNAGC 1 cut(s) 1217
Bpu1102I GCTNAGC 1 cut(s) 573
BsaAI YACGTR 1 cut(s) 385
BsaJI CCNNGG 5 cut(s) 263, 461, 842, 965, 1179
BsaWI WCCGGW 2 cut(s) 41, 1000
Bsc4I CCNNNNNNNGG 2 cut(s) 41, 804
Bse118I RCCGGY 1 cut(s) 151
Bse1I ACTGG 2 cut(s) 997, 1116
Bse21I CCTNAGG 1 cut(s) 792
Bse3DI GCAATG 2 cut(s) 358, 1159
BseBI CCWGG 1 cut(s) 843
BseDI CCNNGG 5 cut(s) 263, 461, 842, 965, 1179
BseGI GGATG 4 cut(s) 78, 532, 736, 913
BseLI CCNNNNNNNGG 2 cut(s) 41, 804
BseMI GCAATG 2 cut(s) 358, 1159
BseMII CTCAG 4 cut(s) 335, 593, 723, 783
BseNI ACTGG 2 cut(s) 997, 1116
BseRI GAGGAG 1 cut(s) 738
BseXI GCAGC 8 cut(s) 250, 322, 678, 848, 1100, 1110, 1178, 1246
BsgI GTGCAG 1 cut(s) 899
Bsh1236I CGCG 2 cut(s) 463, 1095
BshFI GGCC 4 cut(s) 797, 1043, 1177, 1184
BshNI GGYRCC 1 cut(s) 1112
BsiHKAI GWGCWC 1 cut(s) 80
BsiSI CCGG 3 cut(s) 42, 152, 1001
BslI CCNNNNNNNGG 2 cut(s) 41, 804
BsmAI GTCTC 1 cut(s) 821
BsmBI CGTCTC 1 cut(s) 821
BsnI GGCC 4 cut(s) 797, 1043, 1177, 1184
Bsp1286I GDGCHC 1 cut(s) 80
Bsp1407I TGTACA 2 cut(s) 479, 549
Bsp143I GATC 5 cut(s) 518, 534, 593, 643, 1047
Bsp1720I GCTNAGC 1 cut(s) 573
BspACI CCGC 6 cut(s) 110, 314, 461, 463, 1095, 1101
BspANI GGCC 4 cut(s) 797, 1043, 1177, 1184
BspCNI CTCAG 4 cut(s) 334, 592, 722, 784
BspFNI CGCG 2 cut(s) 463, 1095
BspLI GGNNCC 2 cut(s) 46, 1114
BspMAI CTGCAG 2 cut(s) 696, 1090
BspMI ACCTGC 1 cut(s) 1135
BspT107I GGYRCC 1 cut(s) 1112
BsrBI CCGCTC 1 cut(s) 110
BsrDI GCAATG 2 cut(s) 358, 1159
BsrFI RCCGGY 1 cut(s) 151
BsrGI TGTACA 2 cut(s) 479, 549
BsrI ACTGG 2 cut(s) 997, 1116
BssAI RCCGGY 1 cut(s) 151
BssECI CCNNGG 5 cut(s) 263, 461, 842, 965, 1179
BssMI GATC 5 cut(s) 518, 534, 593, 643, 1047
BssT1I CCWWGG 3 cut(s) 263, 965, 1179
Bst2UI CCWGG 1 cut(s) 843
Bst6I CTCTTC 2 cut(s) 30, 1230
BstAUI TGTACA 2 cut(s) 479, 549
BstBAI YACGTR 1 cut(s) 385
BstC8I GCNNGC 3 cut(s) 101, 1023, 1093
BstDEI CTNAG 6 cut(s) 321, 573, 579, 709, 792, 1217
BstDSI CCRYGG 1 cut(s) 461
BstF5I GGATG 4 cut(s) 78, 532, 736, 913
BstFNI CGCG 2 cut(s) 463, 1095
BstKTI GATC 5 cut(s) 521, 537, 596, 646, 1050
BstMAI GTCTC 1 cut(s) 821
BstMBI GATC 5 cut(s) 518, 534, 593, 643, 1047
BstMWI GCNNNNNNNGC 1 cut(s) 1218
BstNI CCWGG 1 cut(s) 843
BstNSI RCATGY 1 cut(s) 1016
BstSCI CCNGG 1 cut(s) 841
BstSFI CTRYAG 2 cut(s) 692, 1086
BstUI CGCG 2 cut(s) 463, 1095
BstV1I GCAGC 8 cut(s) 250, 322, 678, 848, 1100, 1110, 1178, 1246
Bsu36I CCTNAGG 1 cut(s) 792
BsuI GTATCC 2 cut(s) 404, 1292
BsuRI GGCC 4 cut(s) 797, 1043, 1177, 1184
BtgI CCRYGG 1 cut(s) 461
BtsCI GGATG 4 cut(s) 78, 532, 736, 913
BtsIMutI CAGTG 2 cut(s) 132, 1167
BveI ACCTGC 1 cut(s) 1135
Cac8I GCNNGC 3 cut(s) 101, 1023, 1093
CaiI CAGNNNCTG 2 cut(s) 842, 1149
Cfr10I RCCGGY 1 cut(s) 151
Cfr13I GGNCC 2 cut(s) 1041, 1176
Cfr42I CCGCGG 1 cut(s) 464
Csp6I GTAC 4 cut(s) 480, 550, 984, 1119
CviAII CATG 4 cut(s) 199, 569, 1013, 1076
CviQI GTAC 4 cut(s) 480, 550, 984, 1119
DdeI CTNAG 6 cut(s) 321, 573, 579, 709, 792, 1217
DpnI GATC 5 cut(s) 520, 536, 595, 645, 1049
DpnII GATC 5 cut(s) 518, 534, 593, 643, 1047
Eam1104I CTCTTC 2 cut(s) 30, 1230
EarI CTCTTC 2 cut(s) 30, 1230
EciI GGCGGA 1 cut(s) 303
Eco130I CCWWGG 3 cut(s) 263, 965, 1179
Eco57I CTGAAG 1 cut(s) 1211
Eco81I CCTNAGG 1 cut(s) 792
EcoO109I RGGNCCY 1 cut(s) 1176
EcoRII CCWGG 1 cut(s) 841
EcoT14I CCWWGG 3 cut(s) 263, 965, 1179
ErhI CCWWGG 3 cut(s) 263, 965, 1179
Esp3I CGTCTC 1 cut(s) 821
FaeI CATG 4 cut(s) 202, 572, 1016, 1079
FatI CATG 4 cut(s) 198, 568, 1012, 1075
FbaI TGATCA 2 cut(s) 643, 1047
FblI GTMKAC 1 cut(s) 584
Fnu4HI GCNGC 9 cut(s) 239, 336, 692, 837, 1089, 1096, 1099, 1167, 1235
FokI GGATG 4 cut(s) 85, 539, 743, 920
Fsp4HI GCNGC 9 cut(s) 239, 336, 692, 837, 1089, 1096, 1099, 1167, 1235
FspBI CTAG 2 cut(s) 867, 1122
GluI GCNGC 9 cut(s) 239, 336, 692, 837, 1089, 1096, 1099, 1167, 1235
HaeIII GGCC 4 cut(s) 797, 1043, 1177, 1184
HapII CCGG 3 cut(s) 42, 152, 1001
Hin1II CATG 4 cut(s) 202, 572, 1016, 1079
HincII GTYRAC 3 cut(s) 233, 585, 1141
HindII GTYRAC 3 cut(s) 233, 585, 1141
HinfI GANTC 4 cut(s) 55, 218, 376, 701
HpaII CCGG 3 cut(s) 42, 152, 1001
HphI GGTGA 1 cut(s) 1015
Hpy166II GTNNAC 6 cut(s) 132, 233, 480, 550, 585, 1141
Hpy188I TCNGA 9 cut(s) 36, 307, 324, 349, 403, 643, 1135, 1230, 1246
Hpy188III TCNNGA 2 cut(s) 905, 1031
Hpy8I GTNNAC 6 cut(s) 132, 233, 480, 550, 585, 1141
HpyAV CCTTC 5 cut(s) 109, 502, 802, 943, 1055
HpyCH4IV ACGT 2 cut(s) 384, 830
HpyCH4V TGCA 7 cut(s) 363, 694, 836, 880, 1088, 1152, 1279
HpyF10VI GCNNNNNNNGC 1 cut(s) 1218
HpyF3I CTNAG 6 cut(s) 321, 573, 579, 709, 792, 1217
HpySE526I ACGT 2 cut(s) 384, 830
Hsp92II CATG 4 cut(s) 202, 572, 1016, 1079
Ksp22I TGATCA 2 cut(s) 643, 1047
KspI CCGCGG 1 cut(s) 464
Kzo9I GATC 5 cut(s) 518, 534, 593, 643, 1047
LmnI GCTCC 8 cut(s) 44, 107, 118, 343, 770, 844, 969, 1018
Lsp1109I GCAGC 8 cut(s) 250, 322, 678, 848, 1100, 1110, 1178, 1246
LweI GCATC 1 cut(s) 1034
MaeI CTAG 2 cut(s) 867, 1122
MaeII ACGT 2 cut(s) 384, 830
MaeIII GTNAC 3 cut(s) 685, 758, 1292
MalI GATC 5 cut(s) 520, 536, 595, 645, 1049
MbiI CCGCTC 1 cut(s) 110
MboI GATC 5 cut(s) 518, 534, 593, 643, 1047
MboII GAAGA 7 cut(s) 17, 156, 180, 183, 284, 517, 1217
MfeI CAATTG 2 cut(s) 415, 490
MhlI GDGCHC 1 cut(s) 80
MluCI AATT 8 cut(s) 415, 490, 543, 675, 745, 852, 870, 1204
MlyI GAGTC 3 cut(s) 64, 370, 695
MmeI TCCRAC 1 cut(s) 59
MroXI GAANNNNTTC 1 cut(s) 128
MslI CAYNNNNRTG 2 cut(s) 567, 1074
MspA1I CMGCKG 3 cut(s) 463, 1101, 1169
MspI CCGG 3 cut(s) 42, 152, 1001
MspR9I CCNGG 1 cut(s) 843
MunI CAATTG 2 cut(s) 415, 490
MvaI CCWGG 1 cut(s) 843
MvnI CGCG 2 cut(s) 463, 1095
MwoI GCNNNNNNNGC 1 cut(s) 1218
NdeII GATC 5 cut(s) 518, 534, 593, 643, 1047
NlaIII CATG 4 cut(s) 202, 572, 1016, 1079
NlaIV GGNNCC 2 cut(s) 46, 1114
NmuCI GTSAC 1 cut(s) 685
NspI RCATGY 1 cut(s) 1016
PciI ACATGT 1 cut(s) 1012
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 1 cut(s) 218
PflMI CCANNNNNTGG 1 cut(s) 804
PkrI GCNGC 9 cut(s) 240, 337, 693, 838, 1090, 1097, 1100, 1168, 1236
PleI GAGTC 3 cut(s) 63, 370, 695
PpsI GAGTC 3 cut(s) 63, 370, 695
Ppu21I YACGTR 1 cut(s) 385
PscI ACATGT 1 cut(s) 1012
Psp6I CCWGG 1 cut(s) 841
PspGI CCWGG 1 cut(s) 841
PspN4I GGNNCC 2 cut(s) 46, 1114
PspPI GGNCC 2 cut(s) 1041, 1176
PstI CTGCAG 2 cut(s) 696, 1090
PstNI CAGNNNCTG 2 cut(s) 842, 1149
PvuII CAGCTG 1 cut(s) 1169
RsaI GTAC 4 cut(s) 481, 551, 985, 1120
RsaNI GTAC 4 cut(s) 480, 550, 984, 1119
RseI CAYNNNNRTG 2 cut(s) 567, 1074
SacII CCGCGG 1 cut(s) 464
SalI GTCGAC 1 cut(s) 583
SatI GCNGC 9 cut(s) 239, 336, 692, 837, 1089, 1096, 1099, 1167, 1235
Sau3AI GATC 5 cut(s) 518, 534, 593, 643, 1047
Sau96I GGNCC 2 cut(s) 1041, 1176
ScaI AGTACT 1 cut(s) 1120
SchI GAGTC 3 cut(s) 64, 370, 695
ScrFI CCNGG 1 cut(s) 843
SduI GDGCHC 1 cut(s) 80
SfaNI GCATC 1 cut(s) 1034
SfcI CTRYAG 2 cut(s) 692, 1086
Sfr303I CCGCGG 1 cut(s) 464
SgrBI CCGCGG 1 cut(s) 464
SmiMI CAYNNNNRTG 2 cut(s) 567, 1074
Sse9I AATT 8 cut(s) 415, 490, 543, 675, 745, 852, 870, 1204
SsiI CCGC 6 cut(s) 110, 314, 461, 463, 1095, 1101
SspMI CTAG 2 cut(s) 867, 1122
StyD4I CCNGG 1 cut(s) 841
StyI CCWWGG 3 cut(s) 263, 965, 1179
TaiI ACGT 2 cut(s) 387, 833
TaqI TCGA 5 cut(s) 53, 66, 374, 584, 656
TasI AATT 8 cut(s) 415, 490, 543, 675, 745, 852, 870, 1204
TatI WGTACW 4 cut(s) 479, 549, 983, 1118
TauI GCSGC 1 cut(s) 1098
TfiI GAWTC 1 cut(s) 218
TscAI CASTG 2 cut(s) 139, 1167
TseFI GTSAC 1 cut(s) 685
TseI GCWGC 8 cut(s) 238, 335, 691, 836, 1088, 1098, 1166, 1234
Tsp45I GTSAC 1 cut(s) 685
TspDTI ATGAA 3 cut(s) 156, 195, 1074
TspRI CASTG 2 cut(s) 139, 1167
Van91I CCANNNNNTGG 1 cut(s) 804
XapI RAATTY 1 cut(s) 745
XceI RCATGY 1 cut(s) 1016
XmiI GTMKAC 1 cut(s) 584
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 2 cut(s) 867, 1122
ZrmI AGTACT 1 cut(s) 1120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.