Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0001456

Physical Location & Seq

Genomic Coordinates Reverse (-)

78062057 .. 78064833

Gene Structure 2777 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000036250

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1866 bp

ATGGCCTCCTCTTCCGGGAGCTTAGACACCTCCGCAAATTCCCACCCGAGCAACTTCACCTTCTCCACCCACCCATTCATGACCACCTCCTTCTCCGACCTCTTATCCTCCGGCGCCGACCCCACAAACCCAGTAGCCACCGCAACCCGAAACTCCGGGTACGGGTTATCGGATCGTATTGCTGAGCGAACCGGGTCGGGTGTCCCCAAGTTCAAGTCCCTCCCGCCTCCCCAGCTCCCCATTTCGCCTCCCTCTGTTGTGTCTCCCTCTTCCTACTTTGCAATCCCACCTGGGATGAGCCCCGCCGAGCTTCTCGACTCTCCTGTGCTTCTCAGCTCTTCCAACATTCTTCCATCTCCAACTACTGGGAGTTTTGCAGCTCAAGCCTTTAATTGGAGGATCAATTCTGGTTCTGGTACTAACAACAATAACAACAACCCGAGTGTTAAAGAGGAGCACAAGAACTACTCAGATTTCTCTTTCCAAACCCAAACCAGGGCCCCTCCAACTTCATCATCAGCAATTTTCCAATCCTCAATTCAGACGGCACAAGAGCAGGCATGGAATAACTATCTAGGACCTCCCAAACAAGATAACTTTTCTTCAGGGAAGAGTATGGTCAAACAAGAGTATGGTTCTTCAGTGCAGAGCTTCTCTTCTGAAATGGGGACCAATATGCAGAGCAATAACAATGCTCAAACCAACAGCAGTGGTGGTTTCCAATCAGATCAGTACAGCAACTACAATAACAACAACAACAGCAACAACAACCAGCTGCAATCCCATCAGTGCTTGAACAGGAGATCAGATGATGGGTTCAATTGGAGGAAATATGGCCAGAAACAAGTGAAGGGAAGCGAAAATCCGCGGAGCTATTACAAGTGTACTTATCCGAATTGCCCAACTAAGAAAAAGGTTGAGAGGTCGTTGGATGGACAAATCACTGAGATAGTTTACAAGGGTAATCATAACCATCCCAAGCCACAGAATACTAGAAGGTCGGGTTCATCAGCGGCTTCTTCCCATGCAATTCAGGCTTCCAATCACACCACCAATGACATCCCGGATCAGTCTTTCGGCTCTCATGGTAATTCACAAATGGATAACTCCATTGGAACACCGGAGAATTCATCCAATTTTTCAAATGGAGATGATGAGTTTGATCAGAGCTCTCAGAGAAGCAAGTCAGGAGGAGGGGATGAGTACGATGAGGATGAGCCTAATGCCAAAAGATGGAAAAAAGAAGGTGACAATGAATGTATTTCAGCACCTGGGAGTAGAACAGTAAGAGAACCAAGAGTTGTAGTTCAAACAACAAGTGACATTGATATTCTTGATGATGGCTACAGGTGGAGGAAGTATGGTCAGAAAGTAGTAAAGGGCAACCCAAATCCAAGGAGCTACTACAAGTGCACAAATCCAGGCTGTCCAGTGAGGAAGCATGTAGAGCGAGCTTCACATGATCTTCGAGCAGTGATCACAACCTACGAAGGGAAGCACAACCACGATGTTCCTGCAGCCCGTGGCAGTGGTTCCGCCAACAGGCCATTACCAAGCCACAATACCAACAACAACAACATGAACAACAACCACAATGTACCCACAGCAATGAGGCCTATGACCCATCACAGTACTAACAATGCTGCTAACAACAACCCTCTTGACAATGTAAGGCTACCGACATCAGAAGGGCAAGCACCCTTTACTCTCGAGATGTTGCAGAGCCCAGCAAGTTATGGATTGGCAGGGTATGATAACCCCATGGGGTCATACATGAACCAAACACAGCTCAATGACAACAGCATGTTTTCTAAAACCAAGGACGAGCCAAGAGATGACGCATTTTTCGAATCATTGCTATGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006725 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009409 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009700 GO:0009889 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010035 GO:0010120 GO:0010200 GO:0010243 GO:0010286 GO:0010468 GO:0010506 GO:0010508 GO:0010556 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019748 GO:0031323 GO:0031325 GO:0031326 GO:0031329 GO:0031331 GO:0033554 GO:0034605 GO:0034641 GO:0042221 GO:0042430 GO:0042435 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044424 GO:0044464 GO:0044550 GO:0046217 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0065007 GO:0070370 GO:0071704 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

WRKY

Protein Analysis

622

Amino Acids

67.98

Weight (kDa)

6.99

Isoelectric Point (pI)

51.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	270 - 326	1.6e-26	WRKY DNA -binding domain
WRKY	PF03106	446 - 503	2.3e-26	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001456)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07100 AT5G07100 AT5G07100 AT5G07100 AT5G07100
fragaria_vesca	FvH4_3g39850 FvH4_3g39850 FvH4_3g39850 FvH4_3g39850 FvH4_3g39850 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510
malus_domestica	MD03G1057400.v1.1 MD04G1167700.v1.1 MD06G1115200.v1.1 MD11G1059400.v1.1 MD12G1181000.v1.1
prunus_persica	Prupe.6G046900_v2.0.a1 Prupe.6G286000_v2.0.a1
pyrus_communis	pycom03g04580 pycom04g14890 pycom06g10790 pycom11g04930 pycom12g17060
rosa_chinensis	RchiOBHm_Chr3g0461481 RchiOBHm_Chr5g0071811
rosa_laevigata	RLG00000024931 RLG00000036250
rosa_multiflora	Rmu_sc0002677.1_g000005 Rmu_ssc0000127.1_g000054
rosa_roxburghii	Rroxscaffold_1G00009350 Rroxscaffold_6G00418250 Rroxscaffold_6G00418580
rosa_rugosa	Rorug03G0051000 Rorug03G0051100.1 Rorug05G0414700
rosa_samantha	Rh3AG108800 Rh3BG112700 Rh3CG114200 Rh3DG114300 Rh5AG471000 Rh5BG490700 Rh5CG514700 Rh5DG502000
rosa_wichuraiana	Rw3G009250 Rw5G043770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	113
AccB7I	CCANNNNNTGG	2 cut(s)	365, 1233
AccII	CGCG	1 cut(s)	868
AciI	CCGC	8 cut(s)	33, 141, 224, 303, 866, 868, 1013, 1536
AclWI	GGATC	3 cut(s)	180, 407, 1074
AcoI	YGGCCR	1 cut(s)	835
AcsI	RAATTY	2 cut(s)	37, 1126
AcuI	CTGAAG	2 cut(s)	588, 624
AcyI	GRCGYC	1 cut(s)	114
AfaI	GTAC	7 cut(s)	161, 418, 734, 886, 1205, 1599, 1633
AfiI	CCNNNNNNNGG	9 cut(s)	15, 162, 365, 393, 495, 496, 1233, 1491, 1542
AgsI	TTSAA	5 cut(s)	214, 796, 820, 1143, 1310
AjnI	CCWGG	4 cut(s)	289, 494, 1270, 1420
AjuI	GAANNNNNNNTTGG	2 cut(s)	522, 554
Alw21I	GWGCWC	3 cut(s)	459, 1172, 1415
Alw26I	GTCTC	1 cut(s)	267
Alw44I	GTGCAC	1 cut(s)	1411
AlwI	GGATC	3 cut(s)	180, 407, 1074
AlwNI	CAGNNNCTG	1 cut(s)	1271
Ama87I	CYCGRG	3 cut(s)	46, 439, 1709
AoxI	GGCC	5 cut(s)	3, 498, 835, 1544, 1613
ApaI	GGGCCC	1 cut(s)	502
ApaLI	GTGCAC	1 cut(s)	1411
ApeKI	GCWGC	4 cut(s)	377, 775, 1517, 1643
ApoI	RAATTY	2 cut(s)	37, 1126
ArsI	GACNNNNNNTTYG	2 cut(s)	1829, 1861
AspLEI	GCGC	1 cut(s)	116
AspS9I	GGNCC	4 cut(s)	498, 499, 578, 669
AsuC2I	CCSGG	4 cut(s)	16, 157, 193, 1064
AsuHPI	GGTGA	2 cut(s)	49, 1259
AsuII	TTCGAA	1 cut(s)	1848
AvaI	CYCGRG	3 cut(s)	46, 439, 1709
AvaII	GGWCC	2 cut(s)	578, 669
BaeGI	GKGCMC	2 cut(s)	502, 1415
BalI	TGGCCA	1 cut(s)	837
BanI	GGYRCC	1 cut(s)	113
BanII	GRGCYC	4 cut(s)	302, 502, 1172, 1727
Bbv12I	GWGCWC	3 cut(s)	459, 1172, 1415
BbvI	GCAGC	4 cut(s)	389, 762, 1529, 1630
BccI	CCATC	8 cut(s)	361, 792, 806, 926, 981, 1227, 1334, 1632
BceAI	ACGGC	1 cut(s)	561
BcgI	CGANNNNNNTGC	2 cut(s)	1496, 1530
BciT130I	CCWGG	4 cut(s)	291, 496, 1272, 1422
BclI	TGATCA	2 cut(s)	1162, 1476
BcnI	CCSGG	4 cut(s)	16, 157, 193, 1064
BcoDI	GTCTC	1 cut(s)	267
BfaI	CTAG	2 cut(s)	575, 993
BfmI	CTRYAG	2 cut(s)	1345, 1515
BfoI	RGCGCY	1 cut(s)	117
BisI	GCNGC	5 cut(s)	378, 776, 1014, 1518, 1644
BlpI	GCTNAGC	1 cut(s)	183
BlsI	GCNGC	5 cut(s)	379, 777, 1015, 1519, 1645
BmcAI	AGTACT	1 cut(s)	1633
Bme1390I	CCNGG	8 cut(s)	16, 157, 193, 291, 496, 1064, 1272, 1422
Bme18I	GGWCC	2 cut(s)	578, 669
BmeT110I	CYCGRG	3 cut(s)	46, 439, 1709
BmgT120I	GGNCC	4 cut(s)	498, 499, 578, 669
BmiI	GGNNCC	5 cut(s)	115, 500, 501, 670, 1534
BmrFI	CCNGG	8 cut(s)	16, 157, 193, 291, 496, 1064, 1272, 1422
BmrI	ACTGGG	2 cut(s)	125, 375
BmuI	ACTGGG	2 cut(s)	125, 375
Bpu1102I	GCTNAGC	1 cut(s)	183
Bpu14I	TTCGAA	1 cut(s)	1848
BpuEI	CTTGAG	1 cut(s)	366
BpuMI	CCSGG	4 cut(s)	16, 157, 193, 1064
BsaHI	GRCGYC	1 cut(s)	114
BsaJI	CCNNGG	8 cut(s)	290, 495, 866, 1271, 1394, 1522, 1761, 1818
BsaWI	WCCGGW	1 cut(s)	1120
Bsc4I	CCNNNNNNNGG	9 cut(s)	15, 162, 365, 393, 495, 496, 1233, 1491, 1542
Bse1I	ACTGG	3 cut(s)	131, 370, 1430
Bse3DI	GCAATG	2 cut(s)	1614, 1853
BseBI	CCWGG	4 cut(s)	291, 496, 1272, 1422
BseDI	CCNNGG	8 cut(s)	290, 495, 866, 1271, 1394, 1522, 1761, 1818
BseGI	GGATG	7 cut(s)	300, 937, 973, 1059, 1130, 1204, 1219
BseLI	CCNNNNNNNGG	9 cut(s)	15, 162, 365, 393, 495, 496, 1233, 1491, 1542
BseMI	GCAATG	2 cut(s)	1614, 1853
BseMII	CTCAG	5 cut(s)	174, 346, 483, 936, 1187
BseNI	ACTGG	3 cut(s)	131, 370, 1430
BseRI	GAGGAG	2 cut(s)	467, 1206
BseSI	GKGCMC	2 cut(s)	502, 1415
BseXI	GCAGC	4 cut(s)	389, 762, 1529, 1630
BseYI	CCCAGC	2 cut(s)	231, 1726
BsgI	GTGCAG	1 cut(s)	665
Bsh1236I	CGCG	1 cut(s)	868
BshFI	GGCC	5 cut(s)	5, 500, 837, 1546, 1615
BshNI	GGYRCC	1 cut(s)	113
BsiHKAI	GWGCWC	3 cut(s)	459, 1172, 1415
BsiHKCI	CYCGRG	3 cut(s)	46, 439, 1709
BsiSI	CCGG	6 cut(s)	15, 111, 156, 192, 1064, 1121
BslFI	GGGAC	3 cut(s)	188, 202, 682
BslI	CCNNNNNNNGG	9 cut(s)	15, 162, 365, 393, 495, 496, 1233, 1491, 1542
BsmAI	GTCTC	1 cut(s)	267
BsmFI	GGGAC	3 cut(s)	188, 202, 682
BsnI	GGCC	5 cut(s)	5, 500, 837, 1546, 1615
BsoBI	CYCGRG	3 cut(s)	46, 439, 1709
Bsp119I	TTCGAA	1 cut(s)	1848
Bsp120I	GGGCCC	1 cut(s)	498
Bsp1286I	GDGCHC	6 cut(s)	302, 459, 502, 1172, 1415, 1727
Bsp143I	GATC	8 cut(s)	172, 399, 727, 803, 1066, 1162, 1462, 1476
Bsp1720I	GCTNAGC	1 cut(s)	183
Bsp19I	CCATGG	1 cut(s)	1761
BspACI	CCGC	8 cut(s)	33, 141, 224, 303, 866, 868, 1013, 1536
BspANI	GGCC	5 cut(s)	5, 500, 837, 1546, 1615
BspCNI	CTCAG	5 cut(s)	175, 345, 482, 937, 1186
BspFNI	CGCG	1 cut(s)	868
BspHI	TCATGA	1 cut(s)	78
BspLI	GGNNCC	5 cut(s)	115, 500, 501, 670, 1534
BspMAI	CTGCAG	1 cut(s)	1519
BspPI	GGATC	3 cut(s)	180, 407, 1074
BspQI	GCTCTTC	1 cut(s)	343
BspT104I	TTCGAA	1 cut(s)	1848
BspT107I	GGYRCC	1 cut(s)	113
BsrDI	GCAATG	2 cut(s)	1614, 1853
BsrI	ACTGG	3 cut(s)	131, 370, 1430
BssECI	CCNNGG	8 cut(s)	290, 495, 866, 1271, 1394, 1522, 1761, 1818
BssMI	GATC	8 cut(s)	172, 399, 727, 803, 1066, 1162, 1462, 1476
BssNI	GRCGYC	1 cut(s)	114
BssT1I	CCWWGG	3 cut(s)	1394, 1761, 1818
Bst2UI	CCWGG	4 cut(s)	291, 496, 1272, 1422
Bst4CI	ACNGT	2 cut(s)	1285, 1631
Bst6I	CTCTTC	5 cut(s)	16, 274, 343, 605, 661
BstACI	GRCGYC	1 cut(s)	114
BstBI	TTCGAA	1 cut(s)	1848
BstC8I	GCNNGC	3 cut(s)	558, 1452, 1695
BstDEI	CTNAG	7 cut(s)	22, 183, 332, 469, 906, 945, 1173
BstDSI	CCRYGG	3 cut(s)	866, 1522, 1761
BstF5I	GGATG	7 cut(s)	300, 937, 973, 1059, 1130, 1204, 1219
BstFNI	CGCG	1 cut(s)	868
BstH2I	RGCGCY	1 cut(s)	117
BstHHI	GCGC	1 cut(s)	116
BstKTI	GATC	8 cut(s)	175, 402, 730, 806, 1069, 1165, 1465, 1479
BstMAI	GTCTC	1 cut(s)	267
BstMBI	GATC	8 cut(s)	172, 399, 727, 803, 1066, 1162, 1462, 1476
BstMWI	GCNNNNNNNGC	4 cut(s)	232, 383, 1034, 1447
BstNI	CCWGG	4 cut(s)	291, 496, 1272, 1422
BstNSI	RCATGY	2 cut(s)	1445, 1807
BstSCI	CCNGG	8 cut(s)	14, 155, 191, 289, 494, 1062, 1270, 1420
BstSFI	CTRYAG	2 cut(s)	1345, 1515
BstSLI	GKGCMC	2 cut(s)	502, 1415
BstUI	CGCG	1 cut(s)	868
BstV1I	GCAGC	4 cut(s)	389, 762, 1529, 1630
BsuRI	GGCC	5 cut(s)	5, 500, 837, 1546, 1615
BtgI	CCRYGG	3 cut(s)	866, 1522, 1761
BtsCI	GGATG	7 cut(s)	300, 937, 973, 1059, 1130, 1204, 1219
BtsI	GCAGTG	3 cut(s)	715, 1479, 1534
BtsIMutI	CAGTG	7 cut(s)	648, 715, 794, 942, 1437, 1479, 1534
Cac8I	GCNNGC	3 cut(s)	558, 1452, 1695
CaiI	CAGNNNCTG	1 cut(s)	1271
CciI	TCATGA	1 cut(s)	78
CfoI	GCGC	1 cut(s)	116
Cfr13I	GGNCC	4 cut(s)	498, 499, 578, 669
Cfr42I	CCGCGG	1 cut(s)	869
CseI	GACGC	1 cut(s)	1847
Csp6I	GTAC	7 cut(s)	160, 417, 733, 885, 1204, 1598, 1632
CspCI	CAANNNNNGTGG	2 cut(s)	691, 726
CviQI	GTAC	7 cut(s)	160, 417, 733, 885, 1204, 1598, 1632
DdeI	CTNAG	7 cut(s)	22, 183, 332, 469, 906, 945, 1173
DinI	GGCGCC	1 cut(s)	115
DpnI	GATC	8 cut(s)	174, 401, 729, 805, 1068, 1164, 1464, 1478
DpnII	GATC	8 cut(s)	172, 399, 727, 803, 1066, 1162, 1462, 1476
EaeI	YGGCCR	1 cut(s)	835
Eam1104I	CTCTTC	5 cut(s)	16, 274, 343, 605, 661
EarI	CTCTTC	5 cut(s)	16, 274, 343, 605, 661
EciI	GGCGGA	1 cut(s)	1525
Ecl136II	GAGCTC	1 cut(s)	1170
Eco130I	CCWWGG	3 cut(s)	1394, 1761, 1818
Eco147I	AGGCCT	1 cut(s)	1615
Eco24I	GRGCYC	4 cut(s)	302, 502, 1172, 1727
Eco47I	GGWCC	2 cut(s)	578, 669
Eco53kI	GAGCTC	1 cut(s)	1170
Eco57I	CTGAAG	2 cut(s)	588, 624
Eco88I	CYCGRG	3 cut(s)	46, 439, 1709
EcoICRI	GAGCTC	1 cut(s)	1170
EcoO109I	RGGNCCY	3 cut(s)	498, 499, 578
EcoRI	GAATTC	1 cut(s)	1126
EcoRII	CCWGG	4 cut(s)	289, 494, 1270, 1420
EcoT14I	CCWWGG	3 cut(s)	1394, 1761, 1818
EcoT38I	GRGCYC	4 cut(s)	302, 502, 1172, 1727
EgeI	GGCGCC	1 cut(s)	115
EheI	GGCGCC	1 cut(s)	115
ErhI	CCWWGG	3 cut(s)	1394, 1761, 1818
FaqI	GGGAC	3 cut(s)	188, 202, 682
FauI	CCCGC	2 cut(s)	231, 310
FbaI	TGATCA	2 cut(s)	1162, 1476
Fnu4HI	GCNGC	5 cut(s)	378, 776, 1014, 1518, 1644
FokI	GGATG	7 cut(s)	307, 944, 960, 1046, 1117, 1211, 1226
FriOI	GRGCYC	4 cut(s)	302, 502, 1172, 1727
Fsp4HI	GCNGC	5 cut(s)	378, 776, 1014, 1518, 1644
FspBI	CTAG	2 cut(s)	575, 993
GlaI	GCGC	1 cut(s)	115
GluI	GCNGC	5 cut(s)	378, 776, 1014, 1518, 1644
GsaI	CCCAGC	2 cut(s)	235, 1730
HaeII	RGCGCY	1 cut(s)	117
HaeIII	GGCC	5 cut(s)	5, 500, 837, 1546, 1615
HapII	CCGG	6 cut(s)	15, 111, 156, 192, 1064, 1121
HgaI	GACGC	1 cut(s)	1847
HhaI	GCGC	1 cut(s)	116
Hin1I	GRCGYC	1 cut(s)	114
Hin6I	GCGC	1 cut(s)	114
HinP1I	GCGC	1 cut(s)	114
HinfI	GANTC	2 cut(s)	317, 1850
HpaII	CCGG	6 cut(s)	15, 111, 156, 192, 1064, 1121
HphI	GGTGA	2 cut(s)	49, 1259
Hpy166II	GTNNAC	3 cut(s)	885, 955, 1413
Hpy188III	TCNNGA	7 cut(s)	79, 314, 1188, 1334, 1661, 1709, 1711
Hpy8I	GTNNAC	3 cut(s)	885, 955, 1413
HpyAV	CCTTC	7 cut(s)	70, 100, 844, 990, 1238, 1484, 1682
HpyCH4III	ACNGT	2 cut(s)	1285, 1631
HpyCH4V	TGCA	9 cut(s)	281, 377, 646, 679, 778, 1028, 1413, 1517, 1720
HpyF10VI	GCNNNNNNNGC	4 cut(s)	232, 383, 1034, 1447
HpyF3I	CTNAG	7 cut(s)	22, 183, 332, 469, 906, 945, 1173
Hsp92I	GRCGYC	1 cut(s)	114
HspAI	GCGC	1 cut(s)	114
KasI	GGCGCC	1 cut(s)	113
Ksp22I	TGATCA	2 cut(s)	1162, 1476
KspI	CCGCGG	1 cut(s)	869
Kzo9I	GATC	8 cut(s)	172, 399, 727, 803, 1066, 1162, 1462, 1476
LguI	GCTCTTC	1 cut(s)	343
LmnI	GCTCC	5 cut(s)	18, 240, 454, 870, 1398
Lsp1109I	GCAGC	4 cut(s)	389, 762, 1529, 1630
MaeI	CTAG	2 cut(s)	575, 993
MaeIII	GTNAC	2 cut(s)	1247, 1319
MalI	GATC	8 cut(s)	174, 401, 729, 805, 1068, 1164, 1464, 1478
MboI	GATC	8 cut(s)	172, 399, 727, 803, 1066, 1162, 1462, 1476
MfeI	CAATTG	1 cut(s)	820
MhlI	GDGCHC	6 cut(s)	302, 459, 502, 1172, 1415, 1727
MlsI	TGGCCA	1 cut(s)	837
MluNI	TGGCCA	1 cut(s)	837
Mly113I	GGCGCC	1 cut(s)	114
MlyI	GAGTC	1 cut(s)	311
MmeI	TCCRAC	5 cut(s)	120, 366, 383, 530, 909
Mox20I	TGGCCA	1 cut(s)	837
MscI	TGGCCA	1 cut(s)	837
MseI	TTAA	2 cut(s)	390, 447
MslI	CAYNNNNRTG	2 cut(s)	1607, 1858
Msp20I	TGGCCA	1 cut(s)	837
MspA1I	CMGCKG	3 cut(s)	775, 868, 1013
MspI	CCGG	6 cut(s)	15, 111, 156, 192, 1064, 1121
MspR9I	CCNGG	8 cut(s)	16, 157, 193, 291, 496, 1064, 1272, 1422
MunI	CAATTG	1 cut(s)	820
MvaI	CCWGG	4 cut(s)	291, 496, 1272, 1422
MvnI	CGCG	1 cut(s)	868
MwoI	GCNNNNNNNGC	4 cut(s)	232, 383, 1034, 1447
NarI	GGCGCC	1 cut(s)	114
NciI	CCSGG	4 cut(s)	16, 157, 193, 1064
NcoI	CCATGG	1 cut(s)	1761
NdeII	GATC	8 cut(s)	172, 399, 727, 803, 1066, 1162, 1462, 1476
NlaIV	GGNNCC	5 cut(s)	115, 500, 501, 670, 1534
NmeAIII	GCCGAG	1 cut(s)	331
NmuCI	GTSAC	2 cut(s)	1247, 1319
NspI	RCATGY	2 cut(s)	1445, 1807
NspV	TTCGAA	1 cut(s)	1848
PaeR7I	CTCGAG	1 cut(s)	1709
PagI	TCATGA	1 cut(s)	78
PceI	AGGCCT	1 cut(s)	1615
PciSI	GCTCTTC	1 cut(s)	343
PcsI	WCGNNNNNNNCGW	1 cut(s)	1845
PfeI	GAWTC	1 cut(s)	1850
PflMI	CCANNNNNTGG	2 cut(s)	365, 1233
PfoI	TCCNGGA	2 cut(s)	14, 1062
PkrI	GCNGC	5 cut(s)	379, 777, 1015, 1519, 1645
PleI	GAGTC	1 cut(s)	311
PluTI	GGCGCC	1 cut(s)	117
PpsI	GAGTC	1 cut(s)	311
PpuMI	RGGWCCY	1 cut(s)	578
Psp124BI	GAGCTC	1 cut(s)	1172
Psp5II	RGGWCCY	1 cut(s)	578
Psp6I	CCWGG	4 cut(s)	289, 494, 1270, 1420
PspFI	CCCAGC	2 cut(s)	231, 1726
PspGI	CCWGG	4 cut(s)	289, 494, 1270, 1420
PspN4I	GGNNCC	5 cut(s)	115, 500, 501, 670, 1534
PspOMI	GGGCCC	1 cut(s)	498
PspPI	GGNCC	4 cut(s)	498, 499, 578, 669
PspPPI	RGGWCCY	1 cut(s)	578
PstI	CTGCAG	1 cut(s)	1519
PstNI	CAGNNNCTG	1 cut(s)	1271
PvuII	CAGCTG	1 cut(s)	775
RsaI	GTAC	7 cut(s)	161, 418, 734, 886, 1205, 1599, 1633
RsaNI	GTAC	7 cut(s)	160, 417, 733, 885, 1204, 1598, 1632
RseI	CAYNNNNRTG	2 cut(s)	1607, 1858
SacI	GAGCTC	1 cut(s)	1172
SacII	CCGCGG	1 cut(s)	869
SapI	GCTCTTC	1 cut(s)	343
SaqAI	TTAA	2 cut(s)	390, 447
SatI	GCNGC	5 cut(s)	378, 776, 1014, 1518, 1644
Sau3AI	GATC	8 cut(s)	172, 399, 727, 803, 1066, 1162, 1462, 1476
Sau96I	GGNCC	4 cut(s)	498, 499, 578, 669
ScaI	AGTACT	1 cut(s)	1633
SchI	GAGTC	1 cut(s)	311
ScrFI	CCNGG	8 cut(s)	16, 157, 193, 291, 496, 1064, 1272, 1422
SduI	GDGCHC	6 cut(s)	302, 459, 502, 1172, 1415, 1727
SfcI	CTRYAG	2 cut(s)	1345, 1515
SfoI	GGCGCC	1 cut(s)	115
Sfr274I	CTCGAG	1 cut(s)	1709
Sfr303I	CCGCGG	1 cut(s)	869
SfuI	TTCGAA	1 cut(s)	1848
SgrBI	CCGCGG	1 cut(s)	869
SinI	GGWCC	2 cut(s)	578, 669
SlaI	CTCGAG	1 cut(s)	1709
SmiMI	CAYNNNNRTG	2 cut(s)	1607, 1858
SmlI	CTYRAG	2 cut(s)	381, 1709
SmoI	CTYRAG	2 cut(s)	381, 1709
SseBI	AGGCCT	1 cut(s)	1615
SsiI	CCGC	8 cut(s)	33, 141, 224, 303, 866, 868, 1013, 1536
SspDI	GGCGCC	1 cut(s)	113
SspMI	CTAG	2 cut(s)	575, 993
SstI	GAGCTC	1 cut(s)	1172
StuI	AGGCCT	1 cut(s)	1615
StyD4I	CCNGG	8 cut(s)	14, 155, 191, 289, 494, 1062, 1270, 1420
StyI	CCWWGG	3 cut(s)	1394, 1761, 1818
TaaI	ACNGT	2 cut(s)	1285, 1631
TaqI	TCGA	4 cut(s)	315, 1468, 1710, 1848
TatI	WGTACW	3 cut(s)	732, 884, 1631
TauI	GCSGC	1 cut(s)	1016
TfiI	GAWTC	1 cut(s)	1850
Tru1I	TTAA	2 cut(s)	390, 447
Tru9I	TTAA	2 cut(s)	390, 447
TscAI	CASTG	7 cut(s)	648, 715, 794, 949, 1437, 1479, 1534
TseFI	GTSAC	2 cut(s)	1247, 1319
TseI	GCWGC	4 cut(s)	377, 775, 1517, 1643
Tsp45I	GTSAC	2 cut(s)	1247, 1319
TspDTI	ATGAA	7 cut(s)	67, 501, 996, 1119, 1269, 1595, 1790
TspRI	CASTG	7 cut(s)	648, 715, 794, 949, 1437, 1479, 1534
Van91I	CCANNNNNTGG	2 cut(s)	365, 1233
VneI	GTGCAC	1 cut(s)	1411
VpaK11BI	GGWCC	2 cut(s)	578, 669
XapI	RAATTY	2 cut(s)	37, 1126
XceI	RCATGY	2 cut(s)	1445, 1807
XhoI	CTCGAG	1 cut(s)	1709
XspI	CTAG	2 cut(s)	575, 993
ZrmI	AGTACT	1 cut(s)	1633

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.