RchiOBHm_Chr3g0461481
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001456
Physical Location & Seq
Forward (+)
9532184 .. 9534606
2423 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42789

Sequence Viewer

Length: 1521 bp
ATGACTTCTTCCTTCACTCACCTCCTCACAAGCAACATGAACATGAACAATTTTGACCAGGACCGAACCGGCAGCTGGAGCTGGGGACTCTCCGACCGTTCAACGGAAGCCGAAATCCCCAAGTTCAAGTCACTCCAGCCGCCTTCTCTCCCTCTCTCTCCTCCTCCTGCTTCCCCTTCTTCTTACTTGGTTAACAACCCTGGTTTTAGCCCTACTGATTTTTTCAGCTCACCCATGTTTCTTACTCACTCAAATACTCTTCCATCTCCGACCACCGGAGCCTTTTCGAGTCAGATTTTCGATTGGATGAGCACTCCTAAAGAAAATAACAGCCAGCATGGAGCGGAGAAGGAGCAGAAAATGTTCGCTGATTTCTCATTTCAACCGGCAACATCGTCTTCATCGTTCAGTGTTCAGCAATCTTCTTCAAGCATGGTTTCATCGGTGGAGGAATCATTGAAAAGTCAACAGCAGCAATCGTGGGGTTTCAACAACCCAAGGCAAGAAGAAAGCGTAGCAGAGAAAACAGAAGTGAAATCCGAGTTTGCGCCAACTCAGAGCTTCAATGCTGCTCCAAAGTCTGATTACACTCATTCGACCCAGTATGTGAGGGAACAGAAATCAGATGATGGATACAATTGGAGAAAATATGGACAGAAGCAAGTGAAAGGTAGCGAAAATCCTCGGAGTTATTACAAGTGTACATTTCCCAGTTGCCCAACAAAGAAGAAGGTGGAGAGATCATTGGATGGACAGATCACTCAAATTGTGTACAAGGGAAGTCACAACCATGCTAAGCCTCAGTCGACAAGACGATCAGCCTCTCAACAACAATCGCTTCAAACAAGTTCGTATGGGATTTCTGATCAGTCTGCCTCGACAATATCCAATCCAAAAATCGAAGCTGTCACGCTGCAGGAGGACTCTAACTCAGCCTCAATGGGAGGAGAGGATGAGTTTGAACAAAATTCGCCGATAAGTAACTCAAATGGAGCTGATGATGACAATGAGCCTGAGGCCAAAAGATGGAAGGGAGAAAATGCAAATGAGACGTATGCAGCTCCTGGGAGTAGAATTGTGAAAGAACCTAGAATTGTAGTGCAGACCACAAGTGAGATTGATATTCTGGATGATGGGTATAGATGGAGGAAATATGGACAGAAAGTAGTGAAGGGAAATCCAAACCCAAGGAGCTACTACAAATGTACTTCTACTGGCTGTCCAGTGAGGAAACATGTGGAGCGAGCATCTCACGATACGAGGGCCGTGATCACAACTTATGAAGGGAAGCACAACCACGATGTTCCTGCAGCACGTGGCAGCGGTGGTTATGGTACTAGACCTGCTTCTGATGCCAACAGCAACAGCAATGTATCAGTGGCAGCTGTGAGGCCCTTGGCCTTGCCTAATCATTCTAACAATTTGAGCTACCTAAGTTCTCTTCAGAAGCAGCCAACATCAGAAAGCCAAGCACCTTATACTCTCAAAATGTTGCAGAACACAGGAAGTTACGGATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
WRKY

Protein Analysis

506

Amino Acids

55.93

Weight (kDa)

7.67

Isoelectric Point (pI)

60.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 209 - 264 7.3e-26 WRKY DNA -binding domain
WRKY PF03106 377 - 434 1.9e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1351
AccB7I CCANNNNNTGG 1 cut(s) 1026
AccBSI CCGCTC 1 cut(s) 344
AccI GTMKAC 1 cut(s) 806
AciI CCGC 3 cut(s) 140, 344, 1323
AcsI RAATTY 1 cut(s) 967
AcuI CTGAAG 1 cut(s) 1427
AcvI CACGTG 1 cut(s) 1316
AfaI GTAC 4 cut(s) 703, 773, 1207, 1336
AfiI CCNNNNNNNGG 4 cut(s) 75, 103, 275, 1026
AflIII ACRYGT 1 cut(s) 1234
AgsI TTSAA 9 cut(s) 102, 127, 383, 429, 460, 490, 565, 842, 962
AjnI CCWGG 3 cut(s) 57, 199, 1063
Alw21I GWGCWC 1 cut(s) 314
Alw26I GTCTC 1 cut(s) 1043
AlwNI CAGNNNCTG 1 cut(s) 1064
AoxI GGCC 4 cut(s) 1017, 1263, 1391, 1398
ApeKI GCWGC 9 cut(s) 72, 472, 569, 913, 1058, 1310, 1320, 1382, 1450
ApoI RAATTY 1 cut(s) 967
Asp700I GAANNNNTTC 1 cut(s) 362
AspLEI GCGC 1 cut(s) 550
AspS9I GGNCC 3 cut(s) 61, 1263, 1392
AsuHPI GGTGA 2 cut(s) 11, 222
AvaII GGWCC 1 cut(s) 61
AxyI CCTNAGG 1 cut(s) 1014
BbrPI CACGTG 1 cut(s) 1316
BbsI GAAGAC 1 cut(s) 390
Bbv12I GWGCWC 1 cut(s) 314
BbvI GCAGC 9 cut(s) 84, 484, 556, 900, 1070, 1322, 1332, 1394, 1462
BccI CCATC 6 cut(s) 271, 623, 743, 1020, 1127, 1137
BceAI ACGGC 1 cut(s) 1250
BcgI CGANNNNNNTGC 2 cut(s) 1289, 1323
BciT130I CCWGG 3 cut(s) 59, 201, 1065
BciVI GTATCC 2 cut(s) 626, 1508
BclI TGATCA 2 cut(s) 865, 1269
BcoDI GTCTC 1 cut(s) 1043
BfaI CTAG 2 cut(s) 1089, 1338
BfmI CTRYAG 2 cut(s) 914, 1308
BfuAI ACCTGC 1 cut(s) 1351
BfuI GTATCC 2 cut(s) 626, 1508
BlpI GCTNAGC 1 cut(s) 795
Bme1390I CCNGG 3 cut(s) 59, 201, 1065
Bme18I GGWCC 1 cut(s) 61
BmgT120I GGNCC 3 cut(s) 61, 1263, 1392
BmiI GGNNCC 1 cut(s) 280
BmrFI CCNGG 3 cut(s) 59, 201, 1065
BmrI ACTGGG 2 cut(s) 595, 705
BmsI GCATC 2 cut(s) 1256, 1342
BmuI ACTGGG 2 cut(s) 595, 705
BpiI GAAGAC 1 cut(s) 390
BpmI CTGGAG 2 cut(s) 97, 119
Bpu1102I GCTNAGC 1 cut(s) 795
BsaAI YACGTR 1 cut(s) 1316
BsaJI CCNNGG 6 cut(s) 199, 497, 683, 1064, 1187, 1395
BsaWI WCCGGW 1 cut(s) 275
Bsc4I CCNNNNNNNGG 4 cut(s) 75, 103, 275, 1026
Bse118I RCCGGY 2 cut(s) 68, 385
Bse1I ACTGG 4 cut(s) 601, 711, 1219, 1223
Bse21I CCTNAGG 1 cut(s) 1014
Bse3DI GCAATG 1 cut(s) 1375
BseBI CCWGG 3 cut(s) 59, 201, 1065
BseDI CCNNGG 6 cut(s) 199, 497, 683, 1064, 1187, 1395
BseGI GGATG 4 cut(s) 312, 754, 958, 1135
BseLI CCNNNNNNNGG 4 cut(s) 75, 103, 275, 1026
BseMI GCAATG 1 cut(s) 1375
BseMII CTCAG 4 cut(s) 569, 815, 945, 1005
BseNI ACTGG 4 cut(s) 601, 711, 1219, 1223
BseRI GAGGAG 4 cut(s) 14, 150, 153, 960
BseXI GCAGC 9 cut(s) 84, 484, 556, 900, 1070, 1322, 1332, 1394, 1462
BseYI CCCAGC 1 cut(s) 81
BsgI GTGCAG 1 cut(s) 1121
Bsh1285I CGRYCG 1 cut(s) 97
BshFI GGCC 4 cut(s) 1019, 1265, 1393, 1400
BsiEI CGRYCG 1 cut(s) 97
BsiHKAI GWGCWC 1 cut(s) 314
BsiSI CCGG 3 cut(s) 69, 276, 386
BslFI GGGAC 1 cut(s) 99
BslI CCNNNNNNNGG 4 cut(s) 75, 103, 275, 1026
BsmAI GTCTC 1 cut(s) 1043
BsmBI CGTCTC 1 cut(s) 1043
BsmFI GGGAC 1 cut(s) 99
BsnI GGCC 4 cut(s) 1019, 1265, 1393, 1400
Bsp1286I GDGCHC 1 cut(s) 314
Bsp1407I TGTACA 2 cut(s) 701, 771
Bsp143I GATC 5 cut(s) 740, 756, 815, 865, 1269
Bsp1720I GCTNAGC 1 cut(s) 795
BspACI CCGC 3 cut(s) 140, 344, 1323
BspANI GGCC 4 cut(s) 1019, 1265, 1393, 1400
BspCNI CTCAG 4 cut(s) 568, 814, 944, 1006
BspLI GGNNCC 1 cut(s) 280
BspMAI CTGCAG 2 cut(s) 918, 1312
BspMI ACCTGC 1 cut(s) 1351
BsrBI CCGCTC 1 cut(s) 344
BsrDI GCAATG 1 cut(s) 1375
BsrFI RCCGGY 2 cut(s) 68, 385
BsrGI TGTACA 2 cut(s) 701, 771
BsrI ACTGG 4 cut(s) 601, 711, 1219, 1223
BssAI RCCGGY 2 cut(s) 68, 385
BssECI CCNNGG 6 cut(s) 199, 497, 683, 1064, 1187, 1395
BssMI GATC 5 cut(s) 740, 756, 815, 865, 1269
BssT1I CCWWGG 3 cut(s) 497, 1187, 1395
Bst2UI CCWGG 3 cut(s) 59, 201, 1065
Bst4CI ACNGT 1 cut(s) 98
Bst6I CTCTTC 2 cut(s) 264, 1446
BstAUI TGTACA 2 cut(s) 701, 771
BstBAI YACGTR 1 cut(s) 1316
BstC8I GCNNGC 2 cut(s) 335, 1245
BstDEI CTNAG 6 cut(s) 555, 795, 801, 931, 1014, 1433
BstF5I GGATG 4 cut(s) 312, 754, 958, 1135
BstHHI GCGC 1 cut(s) 550
BstKTI GATC 5 cut(s) 743, 759, 818, 868, 1272
BstMAI GTCTC 1 cut(s) 1043
BstMBI GATC 5 cut(s) 740, 756, 815, 865, 1269
BstMCI CGRYCG 1 cut(s) 97
BstMWI GCNNNNNNNGC 2 cut(s) 78, 1352
BstNI CCWGG 3 cut(s) 59, 201, 1065
BstNSI RCATGY 1 cut(s) 1238
BstSCI CCNGG 3 cut(s) 57, 199, 1063
BstSFI CTRYAG 2 cut(s) 914, 1308
BstV1I GCAGC 9 cut(s) 84, 484, 556, 900, 1070, 1322, 1332, 1394, 1462
BstV2I GAAGAC 1 cut(s) 390
Bsu36I CCTNAGG 1 cut(s) 1014
BsuI GTATCC 2 cut(s) 626, 1508
BsuRI GGCC 4 cut(s) 1019, 1265, 1393, 1400
BtsCI GGATG 4 cut(s) 312, 754, 958, 1135
BtsIMutI CAGTG 3 cut(s) 415, 1230, 1383
BveI ACCTGC 1 cut(s) 1351
Cac8I GCNNGC 2 cut(s) 335, 1245
CaiI CAGNNNCTG 1 cut(s) 1064
CfoI GCGC 1 cut(s) 550
Cfr10I RCCGGY 2 cut(s) 68, 385
Cfr13I GGNCC 3 cut(s) 61, 1263, 1392
Csp6I GTAC 4 cut(s) 702, 772, 1206, 1335
CviAII CATG 7 cut(s) 37, 43, 235, 338, 433, 791, 1235
CviQI GTAC 4 cut(s) 702, 772, 1206, 1335
DdeI CTNAG 6 cut(s) 555, 795, 801, 931, 1014, 1433
DpnI GATC 5 cut(s) 742, 758, 817, 867, 1271
DpnII GATC 5 cut(s) 740, 756, 815, 865, 1269
Eam1104I CTCTTC 2 cut(s) 264, 1446
EarI CTCTTC 2 cut(s) 264, 1446
Eco130I CCWWGG 3 cut(s) 497, 1187, 1395
Eco47I GGWCC 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 1427
Eco72I CACGTG 1 cut(s) 1316
Eco81I CCTNAGG 1 cut(s) 1014
EcoO109I RGGNCCY 1 cut(s) 1392
EcoRII CCWGG 3 cut(s) 57, 199, 1063
EcoT14I CCWWGG 3 cut(s) 497, 1187, 1395
ErhI CCWWGG 3 cut(s) 497, 1187, 1395
Esp3I CGTCTC 1 cut(s) 1043
FaeI CATG 7 cut(s) 40, 46, 238, 341, 436, 794, 1238
FaqI GGGAC 1 cut(s) 99
FatI CATG 7 cut(s) 36, 42, 234, 337, 432, 790, 1234
FbaI TGATCA 2 cut(s) 865, 1269
FblI GTMKAC 1 cut(s) 806
FokI GGATG 4 cut(s) 319, 761, 965, 1142
FspBI CTAG 2 cut(s) 1089, 1338
GlaI GCGC 1 cut(s) 549
GsaI CCCAGC 1 cut(s) 85
GsuI CTGGAG 2 cut(s) 97, 119
HaeIII GGCC 4 cut(s) 1019, 1265, 1393, 1400
HapII CCGG 3 cut(s) 69, 276, 386
HhaI GCGC 1 cut(s) 550
Hin1II CATG 7 cut(s) 40, 46, 238, 341, 436, 794, 1238
Hin6I GCGC 1 cut(s) 548
HinP1I GCGC 1 cut(s) 548
HincII GTYRAC 3 cut(s) 193, 467, 807
HindII GTYRAC 3 cut(s) 193, 467, 807
HinfI GANTC 4 cut(s) 87, 289, 452, 923
HpaI GTTAAC 1 cut(s) 193
HpaII CCGG 3 cut(s) 69, 276, 386
HphI GGTGA 2 cut(s) 11, 222
Hpy166II GTNNAC 5 cut(s) 193, 467, 702, 772, 807
Hpy188III TCNNGA 2 cut(s) 1127, 1253
Hpy8I GTNNAC 5 cut(s) 193, 467, 702, 772, 807
HpyAV CCTTC 8 cut(s) 22, 153, 186, 343, 724, 1024, 1165, 1277
HpyCH4III ACNGT 1 cut(s) 98
HpyCH4IV ACGT 2 cut(s) 1052, 1315
HpyCH4V TGCA 6 cut(s) 916, 1043, 1058, 1102, 1310, 1495
HpyF10VI GCNNNNNNNGC 2 cut(s) 78, 1352
HpyF3I CTNAG 6 cut(s) 555, 795, 801, 931, 1014, 1433
HpySE526I ACGT 2 cut(s) 1052, 1315
Hsp92II CATG 7 cut(s) 40, 46, 238, 341, 436, 794, 1238
HspAI GCGC 1 cut(s) 548
Ksp22I TGATCA 2 cut(s) 865, 1269
KspAI GTTAAC 1 cut(s) 193
Kzo9I GATC 5 cut(s) 740, 756, 815, 865, 1269
LmnI GCTCC 9 cut(s) 78, 278, 341, 352, 577, 992, 1066, 1191, 1240
Lsp1109I GCAGC 9 cut(s) 84, 484, 556, 900, 1070, 1322, 1332, 1394, 1462
LweI GCATC 2 cut(s) 1256, 1342
MaeI CTAG 2 cut(s) 1089, 1338
MaeII ACGT 2 cut(s) 1052, 1315
MaeIII GTNAC 5 cut(s) 129, 782, 907, 980, 1508
MalI GATC 5 cut(s) 742, 758, 817, 867, 1271
MbiI CCGCTC 1 cut(s) 344
MboI GATC 5 cut(s) 740, 756, 815, 865, 1269
MboII GAAGA 8 cut(s) 171, 251, 390, 414, 417, 518, 739, 1433
MfeI CAATTG 1 cut(s) 637
MhlI GDGCHC 1 cut(s) 314
MluCI AATT 7 cut(s) 49, 637, 765, 967, 1074, 1092, 1420
MlyI GAGTC 3 cut(s) 81, 298, 917
MmeI TCCRAC 2 cut(s) 117, 293
MroXI GAANNNNTTC 1 cut(s) 362
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 2 cut(s) 41, 789
MspA1I CMGCKG 3 cut(s) 75, 1323, 1385
MspI CCGG 3 cut(s) 69, 276, 386
MspR9I CCNGG 3 cut(s) 59, 201, 1065
MunI CAATTG 1 cut(s) 637
MvaI CCWGG 3 cut(s) 59, 201, 1065
MwoI GCNNNNNNNGC 2 cut(s) 78, 1352
NdeII GATC 5 cut(s) 740, 756, 815, 865, 1269
NlaIII CATG 7 cut(s) 40, 46, 238, 341, 436, 794, 1238
NlaIV GGNNCC 1 cut(s) 280
NmuCI GTSAC 3 cut(s) 129, 782, 907
NspI RCATGY 1 cut(s) 1238
PciI ACATGT 1 cut(s) 1234
PcsI WCGNNNNNNNCGW 1 cut(s) 401
PdmI GAANNNNTTC 1 cut(s) 362
PfeI GAWTC 1 cut(s) 452
PflMI CCANNNNNTGG 1 cut(s) 1026
PleI GAGTC 3 cut(s) 81, 297, 917
PmaCI CACGTG 1 cut(s) 1316
PmlI CACGTG 1 cut(s) 1316
PpsI GAGTC 3 cut(s) 81, 297, 917
Ppu21I YACGTR 1 cut(s) 1316
PscI ACATGT 1 cut(s) 1234
Psp6I CCWGG 3 cut(s) 57, 199, 1063
PspCI CACGTG 1 cut(s) 1316
PspFI CCCAGC 1 cut(s) 81
PspGI CCWGG 3 cut(s) 57, 199, 1063
PspN4I GGNNCC 1 cut(s) 280
PspPI GGNCC 3 cut(s) 61, 1263, 1392
PstI CTGCAG 2 cut(s) 918, 1312
PstNI CAGNNNCTG 1 cut(s) 1064
PvuII CAGCTG 2 cut(s) 75, 1385
RsaI GTAC 4 cut(s) 703, 773, 1207, 1336
RsaNI GTAC 4 cut(s) 702, 772, 1206, 1335
RseI CAYNNNNRTG 2 cut(s) 41, 789
SalI GTCGAC 1 cut(s) 805
SaqAI TTAA 1 cut(s) 192
Sau3AI GATC 5 cut(s) 740, 756, 815, 865, 1269
Sau96I GGNCC 3 cut(s) 61, 1263, 1392
SchI GAGTC 3 cut(s) 81, 298, 917
ScrFI CCNGG 3 cut(s) 59, 201, 1065
SduI GDGCHC 1 cut(s) 314
SfaNI GCATC 2 cut(s) 1256, 1342
SfcI CTRYAG 2 cut(s) 914, 1308
SinI GGWCC 1 cut(s) 61
SmiMI CAYNNNNRTG 2 cut(s) 41, 789
Sse9I AATT 7 cut(s) 49, 637, 765, 967, 1074, 1092, 1420
SsiI CCGC 3 cut(s) 140, 344, 1323
SspMI CTAG 2 cut(s) 1089, 1338
StyD4I CCNGG 3 cut(s) 57, 199, 1063
StyI CCWWGG 3 cut(s) 497, 1187, 1395
TaaI ACNGT 1 cut(s) 98
TaiI ACGT 2 cut(s) 1055, 1318
TaqI TCGA 6 cut(s) 287, 300, 596, 806, 878, 900
TaqII GACCGA 1 cut(s) 78
TasI AATT 7 cut(s) 49, 637, 765, 967, 1074, 1092, 1420
TatI WGTACW 3 cut(s) 701, 771, 1205
TauI GCSGC 1 cut(s) 142
TfiI GAWTC 1 cut(s) 452
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 3 cut(s) 415, 1230, 1383
TseFI GTSAC 3 cut(s) 129, 782, 907
TseI GCWGC 9 cut(s) 72, 472, 569, 913, 1058, 1310, 1320, 1382, 1450
Tsp45I GTSAC 3 cut(s) 129, 782, 907
TspDTI ATGAA 5 cut(s) 53, 59, 390, 429, 1296
TspGWI ACGGA 1 cut(s) 119
TspRI CASTG 3 cut(s) 415, 1230, 1383
Van91I CCANNNNNTGG 1 cut(s) 1026
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 1 cut(s) 967
XceI RCATGY 1 cut(s) 1238
XmiI GTMKAC 1 cut(s) 806
XmnI GAANNNNTTC 1 cut(s) 362
XspI CTAG 2 cut(s) 1089, 1338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.