MD12G1181000.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001456
Physical Location & Seq
Reverse (-)
26084482 .. 26086791
2310 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1181000.v1.1.491

Sequence Viewer

Length: 1539 bp
ATGACTTCTTCCTTCACTCACCTCCTCACAAGCAACATGGAGAACAGCGGGATTGGACAGGACAGGACTAATTGGGGGGGACTATTCTCGGACTATTCTTCTAATCCGAACCGGTTCGCGGACAGAAACGGGACTGATATCCCCAAGTTCAAGTCACTCCAGCCTCCTTCTCTGCCTCTCTCTCCTCCCCCTGTTTCCCCTTCTTCTTACTTGACTTCCACGCCGGCTTTTAGTCCCACCGATTTTCTCAGCTCACCCATGTTCCTCTCCTCCTCATATAATCTTGAATCTCCAACAACAGGAGCTTTTTCGAGTCAGGTTTTCGATTGGATGAATAATACTAAAGACACCCAGCAAGGAATTAAGGATAAAGAGGAGCCGAAATTGTTCTCCGATTTCTCGTTCCAACCGGAATCGAGGCCTGCTACTAATCTTCAATCTTCTTCTAGCATGGTTTCAGTGGAAGAACCATTCAAAGGAGAAAGGCAAGCATGGGATTTTAGTGCTGAGAAGACGGGAGTGAAGTCCGAATTCGAGCCAATTGAAGCGAATACGCAGAGTAATGGACTTAATGGTGCTCCAAAGTCCGATTACTTGCATTCTAATCAATGTTCGCAATATGCTCGAGAACAAAAATCGGATGACGGGTTCAATTGGAGGAAGTATGGGCAGAAACAAGTGAAGGGTAGCGAAAATCCGCGGAGTTATTACAAGTGCACGTTTCCGAATTGCCCCACAAAGAAGAAGGTTGAGAGATCATTGGATGGACAGATTACTCAAATTGTGTACAAGGGTAATCACAACCATCCTAAGCCTCAGTCTACAAGAAGATCAAGCTCCAACTCGATTCAGGGTTCTTTCTATGGAATCTCTGATCAATCCGTTCCGACGTTATCCAATCCGAAAGTTGAGTCCGTCTCACTACAGGAGGATTCTTCTACCTCAATTGGAGAGGATGAGTTTGAACAAAACTCTCCGATAAGTAATTCAGTAGGAGCTGAAGATGAAAACGAACCTGAGGCGAAAAGATGGAAGGGAGACAATGCAAATGATCAGTCATATGCATCTTCTGGCGGTAGAATTGTGAAAGAACCGAGAATTGTAGTGCAGACAACGAGCGAAATCGATATTCTGGATGACGGGTATAGGTGGAGGAAATATGGACAGAAAGTAGTGAAGGGAAATCCAAATCCAAGGAGCTACTACAAATGCACGTCCGTAGGTTGTCCAGTGAGGAAGCATGTGGAAAGAGCATCGCAGGACACAAGGGCGGTGATCACCACGTACGAAGGGAAGCACAACCACGATGTTCCGGCAGCACGAGGCAGCGGGAATTATAGCAATGCAAGTAGACCTGCTGCCGATAATAACAGCAACAATGTGTCCATGGCTGTGAGGCCCTTGCCATTGCCTAACCATTCTAATTTGAGCTACCTCAACTCTCTTCAGAACGCAAGGCAGCCAGCAACCACTGAAAGCCAACCGCCGTATACACTCAAAATGCAGAGTGCAGGAAGTTATGGATTTTCTGAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
WRKY

Protein Analysis

513

Amino Acids

56.72

Weight (kDa)

6.73

Isoelectric Point (pI)

55.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 214 - 270 2.7e-26 WRKY DNA -binding domain
WRKY PF03106 379 - 436 1.9e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1363
AccI GTMKAC 3 cut(s) 821, 1351, 1490
AccII CGCG 2 cut(s) 119, 700
AciI CCGC 8 cut(s) 48, 119, 698, 700, 1074, 1271, 1329, 1484
AcsI RAATTY 1 cut(s) 530
AcuI CTGAAG 2 cut(s) 1020, 1430
AfaI GTAC 2 cut(s) 788, 1286
AfiI CCNNNNNNNGG 3 cut(s) 118, 299, 476
AgeI ACCGGT 1 cut(s) 111
AgsI TTSAA 7 cut(s) 151, 287, 437, 475, 545, 652, 965
AjiI CACGTC 1 cut(s) 1215
AloI GAACNNNNNNTCC 2 cut(s) 632, 664
AluBI AGCT 6 cut(s) 252, 305, 837, 998, 1200, 1431
AluI AGCT 6 cut(s) 252, 305, 837, 998, 1200, 1431
Alw21I GWGCWC 2 cut(s) 580, 719
Alw26I GTCTC 2 cut(s) 922, 1032
Alw44I GTGCAC 1 cut(s) 715
Ama87I CYCGRG 1 cut(s) 624
AoxI GGCC 2 cut(s) 419, 1397
ApaLI GTGCAC 1 cut(s) 715
ApeKI GCWGC 4 cut(s) 1316, 1326, 1358, 1459
ApoI RAATTY 1 cut(s) 530
ArsI GACNNNNNNTTYG 4 cut(s) 896, 928, 1040, 1072
AsiGI ACCGGT 1 cut(s) 111
Asp700I GAANNNNTTC 2 cut(s) 113, 386
AspS9I GGNCC 1 cut(s) 1398
AsuHPI GGTGA 4 cut(s) 11, 246, 1270, 1285
AvaI CYCGRG 1 cut(s) 624
AxyI CCTNAGG 1 cut(s) 1017
BaeGI GKGCMC 1 cut(s) 719
BauI CACGAG 1 cut(s) 1320
BbsI GAAGAC 1 cut(s) 518
Bbv12I GWGCWC 2 cut(s) 580, 719
BbvI GCAGC 4 cut(s) 1328, 1338, 1345, 1471
BccI CCATC 3 cut(s) 758, 813, 1023
BceAI ACGGC 1 cut(s) 1471
BcgI CGANNNNNNTGC 2 cut(s) 605, 639
BclI TGATCA 3 cut(s) 874, 1051, 1275
BcoDI GTCTC 2 cut(s) 922, 1032
BfaI CTAG 1 cut(s) 447
BfmI CTRYAG 1 cut(s) 923
BfuAI ACCTGC 1 cut(s) 1363
BisI GCNGC 4 cut(s) 1317, 1327, 1359, 1460
BlsI GCNGC 4 cut(s) 1318, 1328, 1360, 1461
BmeT110I CYCGRG 1 cut(s) 624
BmgBI CACGTC 1 cut(s) 1215
BmgT120I GGNCC 1 cut(s) 1398
BmiI GGNNCC 1 cut(s) 378
BmsI GCATC 2 cut(s) 1073, 1262
BpiI GAAGAC 1 cut(s) 518
BplI GAGNNNNNCTC 2 cut(s) 902, 934
BpmI CTGGAG 1 cut(s) 143
Bpu10I CCTNAGC 1 cut(s) 810
Bsa29I ATCGAT 1 cut(s) 1125
BsaAI YACGTR 1 cut(s) 1284
BsaJI CCNNGG 3 cut(s) 698, 1193, 1386
BsaWI WCCGGW 2 cut(s) 111, 409
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 299, 476
Bse118I RCCGGY 2 cut(s) 111, 223
Bse1I ACTGG 1 cut(s) 1229
Bse21I CCTNAGG 1 cut(s) 1017
Bse3DI GCAATG 2 cut(s) 1348, 1406
BseCI ATCGAT 1 cut(s) 1125
BseDI CCNNGG 3 cut(s) 698, 1193, 1386
BseGI GGATG 6 cut(s) 336, 646, 769, 805, 961, 1141
BseLI CCNNNNNNNGG 3 cut(s) 118, 299, 476
BseMI GCAATG 2 cut(s) 1348, 1406
BseMII CTCAG 5 cut(s) 262, 498, 830, 1008, 1521
BseNI ACTGG 1 cut(s) 1229
BseRI GAGGAG 5 cut(s) 14, 174, 259, 262, 389
BseSI GKGCMC 1 cut(s) 719
BseXI GCAGC 4 cut(s) 1328, 1338, 1345, 1471
BseYI CCCAGC 1 cut(s) 351
BsgI GTGCAG 2 cut(s) 1127, 1530
Bsh1236I CGCG 2 cut(s) 119, 700
BshFI GGCC 2 cut(s) 421, 1399
BshTI ACCGGT 1 cut(s) 111
BshVI ATCGAT 1 cut(s) 1125
BsiHKAI GWGCWC 2 cut(s) 580, 719
BsiHKCI CYCGRG 1 cut(s) 624
BsiSI CCGG 4 cut(s) 112, 224, 410, 1313
BsiWI CGTACG 1 cut(s) 1284
BslFI GGGAC 3 cut(s) 93, 145, 219
BslI CCNNNNNNNGG 3 cut(s) 118, 299, 476
BsmAI GTCTC 2 cut(s) 922, 1032
BsmBI CGTCTC 1 cut(s) 922
BsmFI GGGAC 3 cut(s) 93, 145, 219
BsmI GAATGC 1 cut(s) 598
BsnI GGCC 2 cut(s) 421, 1399
BsoBI CYCGRG 1 cut(s) 624
Bsp1286I GDGCHC 2 cut(s) 580, 719
Bsp1407I TGTACA 1 cut(s) 786
Bsp143I GATC 5 cut(s) 755, 830, 874, 1051, 1275
Bsp19I CCATGG 1 cut(s) 1386
BspACI CCGC 8 cut(s) 48, 119, 698, 700, 1074, 1271, 1329, 1484
BspANI GGCC 2 cut(s) 421, 1399
BspCNI CTCAG 5 cut(s) 261, 499, 829, 1009, 1522
BspDI ATCGAT 1 cut(s) 1125
BspFNI CGCG 2 cut(s) 119, 700
BspLI GGNNCC 1 cut(s) 378
BspMI ACCTGC 1 cut(s) 1363
BsrDI GCAATG 2 cut(s) 1348, 1406
BsrFI RCCGGY 2 cut(s) 111, 223
BsrGI TGTACA 1 cut(s) 786
BsrI ACTGG 1 cut(s) 1229
BssAI RCCGGY 2 cut(s) 111, 223
BssECI CCNNGG 3 cut(s) 698, 1193, 1386
BssMI GATC 5 cut(s) 755, 830, 874, 1051, 1275
BssNAI GTATAC 1 cut(s) 1491
BssSI CACGAG 1 cut(s) 1320
BssT1I CCWWGG 2 cut(s) 1193, 1386
Bst1107I GTATAC 1 cut(s) 1491
Bst2BI CACGAG 1 cut(s) 1320
Bst6I CTCTTC 1 cut(s) 1449
BstAUI TGTACA 1 cut(s) 786
BstBAI YACGTR 1 cut(s) 1284
BstC8I GCNNGC 4 cut(s) 225, 423, 489, 1464
BstDEI CTNAG 6 cut(s) 248, 507, 810, 816, 1017, 1530
BstDSI CCRYGG 2 cut(s) 698, 1386
BstF5I GGATG 6 cut(s) 336, 646, 769, 805, 961, 1141
BstFNI CGCG 2 cut(s) 119, 700
BstKTI GATC 5 cut(s) 758, 833, 877, 1054, 1278
BstMAI GTCTC 2 cut(s) 922, 1032
BstMBI GATC 5 cut(s) 755, 830, 874, 1051, 1275
BstNSI RCATGY 1 cut(s) 1244
BstSFI CTRYAG 1 cut(s) 923
BstSLI GKGCMC 1 cut(s) 719
BstUI CGCG 2 cut(s) 119, 700
BstV1I GCAGC 4 cut(s) 1328, 1338, 1345, 1471
BstV2I GAAGAC 1 cut(s) 518
BstZ17I GTATAC 1 cut(s) 1491
Bsu15I ATCGAT 1 cut(s) 1125
Bsu36I CCTNAGG 1 cut(s) 1017
BsuRI GGCC 2 cut(s) 421, 1399
BsuTUI ATCGAT 1 cut(s) 1125
BtgI CCRYGG 2 cut(s) 698, 1386
BtgZI GCGATG 1 cut(s) 1239
BtrI CACGTC 1 cut(s) 1215
BtsCI GGATG 6 cut(s) 336, 646, 769, 805, 961, 1141
BtsIMutI CAGTG 3 cut(s) 465, 1236, 1470
BveI ACCTGC 1 cut(s) 1363
Cac8I GCNNGC 4 cut(s) 225, 423, 489, 1464
Cfr10I RCCGGY 2 cut(s) 111, 223
Cfr13I GGNCC 1 cut(s) 1398
Cfr42I CCGCGG 1 cut(s) 701
ClaI ATCGAT 1 cut(s) 1125
Csp6I GTAC 2 cut(s) 787, 1285
CspAI ACCGGT 1 cut(s) 111
CviAII CATG 6 cut(s) 37, 259, 451, 492, 1241, 1387
CviQI GTAC 2 cut(s) 787, 1285
DdeI CTNAG 6 cut(s) 248, 507, 810, 816, 1017, 1530
DpnI GATC 5 cut(s) 757, 832, 876, 1053, 1277
DpnII GATC 5 cut(s) 755, 830, 874, 1051, 1275
Eam1104I CTCTTC 1 cut(s) 1449
EarI CTCTTC 1 cut(s) 1449
Eco130I CCWWGG 2 cut(s) 1193, 1386
Eco147I AGGCCT 1 cut(s) 421
Eco32I GATATC 1 cut(s) 139
Eco57I CTGAAG 2 cut(s) 1020, 1430
Eco81I CCTNAGG 1 cut(s) 1017
Eco88I CYCGRG 1 cut(s) 624
EcoO109I RGGNCCY 1 cut(s) 1398
EcoRI GAATTC 1 cut(s) 530
EcoRV GATATC 1 cut(s) 139
EcoT14I CCWWGG 2 cut(s) 1193, 1386
EcoT22I ATGCAT 1 cut(s) 1066
ErhI CCWWGG 2 cut(s) 1193, 1386
Esp3I CGTCTC 1 cut(s) 922
FaeI CATG 6 cut(s) 40, 262, 454, 495, 1244, 1390
FaqI GGGAC 3 cut(s) 93, 145, 219
FatI CATG 6 cut(s) 36, 258, 450, 491, 1240, 1386
FauI CCCGC 2 cut(s) 41, 1322
FauNDI CATATG 1 cut(s) 1060
FbaI TGATCA 3 cut(s) 874, 1051, 1275
FblI GTMKAC 3 cut(s) 821, 1351, 1490
Fnu4HI GCNGC 4 cut(s) 1317, 1327, 1359, 1460
FokI GGATG 6 cut(s) 343, 653, 776, 792, 968, 1148
Fsp4HI GCNGC 4 cut(s) 1317, 1327, 1359, 1460
FspBI CTAG 1 cut(s) 447
GluI GCNGC 4 cut(s) 1317, 1327, 1359, 1460
GsaI CCCAGC 1 cut(s) 355
GsuI CTGGAG 1 cut(s) 143
HaeIII GGCC 2 cut(s) 421, 1399
HapII CCGG 4 cut(s) 112, 224, 410, 1313
Hin1II CATG 6 cut(s) 40, 262, 454, 495, 1244, 1390
HinfI GANTC 7 cut(s) 287, 313, 413, 847, 867, 911, 932
HpaII CCGG 4 cut(s) 112, 224, 410, 1313
HphI GGTGA 4 cut(s) 11, 246, 1270, 1285
Hpy166II GTNNAC 5 cut(s) 717, 787, 822, 1352, 1491
Hpy188III TCNNGA 3 cut(s) 284, 626, 1133
Hpy8I GTNNAC 5 cut(s) 717, 787, 822, 1352, 1491
Hpy99I CGWCG 1 cut(s) 892
HpyAV CCTTC 8 cut(s) 22, 177, 210, 676, 739, 1027, 1171, 1283
HpyCH4IV ACGT 4 cut(s) 719, 890, 1214, 1283
HpyCH4V TGCA 9 cut(s) 598, 717, 1046, 1064, 1108, 1212, 1346, 1504, 1511
HpyF3I CTNAG 6 cut(s) 248, 507, 810, 816, 1017, 1530
HpySE526I ACGT 4 cut(s) 719, 890, 1214, 1283
Hsp92II CATG 6 cut(s) 40, 262, 454, 495, 1244, 1390
KroI GCCGGC 1 cut(s) 223
KroNI GCCGGC 1 cut(s) 225
Ksp22I TGATCA 3 cut(s) 874, 1051, 1275
KspI CCGCGG 1 cut(s) 701
Kzo9I GATC 5 cut(s) 755, 830, 874, 1051, 1275
LmnI GCTCC 6 cut(s) 302, 376, 583, 842, 995, 1197
Lsp1109I GCAGC 4 cut(s) 1328, 1338, 1345, 1471
LweI GCATC 2 cut(s) 1073, 1262
MaeI CTAG 1 cut(s) 447
MaeII ACGT 4 cut(s) 719, 890, 1214, 1283
MaeIII GTNAC 1 cut(s) 153
MalI GATC 5 cut(s) 757, 832, 876, 1053, 1277
MboI GATC 5 cut(s) 755, 830, 874, 1051, 1275
MfeI CAATTG 3 cut(s) 540, 652, 945
MhlI GDGCHC 2 cut(s) 580, 719
MlyI GAGTC 2 cut(s) 322, 920
MmeI TCCRAC 4 cut(s) 317, 430, 864, 911
Mph1103I ATGCAT 1 cut(s) 1066
MroNI GCCGGC 1 cut(s) 223
MroXI GAANNNNTTC 2 cut(s) 113, 386
MseI TTAA 2 cut(s) 363, 570
MslI CAYNNNNRTG 1 cut(s) 1391
MspA1I CMGCKG 3 cut(s) 48, 700, 1329
MspI CCGG 4 cut(s) 112, 224, 410, 1313
MunI CAATTG 3 cut(s) 540, 652, 945
Mva1269I GAATGC 1 cut(s) 598
MvnI CGCG 2 cut(s) 119, 700
NaeI GCCGGC 1 cut(s) 225
NcoI CCATGG 1 cut(s) 1386
NdeI CATATG 1 cut(s) 1060
NdeII GATC 5 cut(s) 755, 830, 874, 1051, 1275
NgoMIV GCCGGC 1 cut(s) 223
NlaIII CATG 6 cut(s) 40, 262, 454, 495, 1244, 1390
NlaIV GGNNCC 1 cut(s) 378
NmuCI GTSAC 1 cut(s) 153
NsiI ATGCAT 1 cut(s) 1066
NspI RCATGY 1 cut(s) 1244
PaeR7I CTCGAG 1 cut(s) 624
PceI AGGCCT 1 cut(s) 421
PctI GAATGC 1 cut(s) 598
PdiI GCCGGC 1 cut(s) 225
PdmI GAANNNNTTC 2 cut(s) 113, 386
PfeI GAWTC 5 cut(s) 287, 413, 847, 867, 932
Pfl23II CGTACG 1 cut(s) 1284
PinAI ACCGGT 1 cut(s) 111
PkrI GCNGC 4 cut(s) 1318, 1328, 1360, 1461
PleI GAGTC 2 cut(s) 321, 919
PpsI GAGTC 2 cut(s) 321, 919
Ppu21I YACGTR 1 cut(s) 1284
PspFI CCCAGC 1 cut(s) 351
PspLI CGTACG 1 cut(s) 1284
PspN4I GGNNCC 1 cut(s) 378
PspPI GGNCC 1 cut(s) 1398
RsaI GTAC 2 cut(s) 788, 1286
RsaNI GTAC 2 cut(s) 787, 1285
RseI CAYNNNNRTG 1 cut(s) 1391
SacII CCGCGG 1 cut(s) 701
SaqAI TTAA 2 cut(s) 363, 570
SatI GCNGC 4 cut(s) 1317, 1327, 1359, 1460
Sau3AI GATC 5 cut(s) 755, 830, 874, 1051, 1275
Sau96I GGNCC 1 cut(s) 1398
SchI GAGTC 2 cut(s) 322, 920
SduI GDGCHC 2 cut(s) 580, 719
SfaNI GCATC 2 cut(s) 1073, 1262
SfcI CTRYAG 1 cut(s) 923
Sfr274I CTCGAG 1 cut(s) 624
Sfr303I CCGCGG 1 cut(s) 701
SgrBI CCGCGG 1 cut(s) 701
SlaI CTCGAG 1 cut(s) 624
SmiMI CAYNNNNRTG 1 cut(s) 1391
SmlI CTYRAG 1 cut(s) 624
SmoI CTYRAG 1 cut(s) 624
SseBI AGGCCT 1 cut(s) 421
SsiI CCGC 8 cut(s) 48, 119, 698, 700, 1074, 1271, 1329, 1484
SspMI CTAG 1 cut(s) 447
StuI AGGCCT 1 cut(s) 421
StyI CCWWGG 2 cut(s) 1193, 1386
TaiI ACGT 4 cut(s) 722, 893, 1217, 1286
TaqI TCGA 7 cut(s) 311, 324, 416, 534, 625, 845, 1125
TatI WGTACW 1 cut(s) 786
TfiI GAWTC 5 cut(s) 287, 413, 847, 867, 932
Tru1I TTAA 2 cut(s) 363, 570
Tru9I TTAA 2 cut(s) 363, 570
TscAI CASTG 3 cut(s) 465, 1236, 1477
TseFI GTSAC 1 cut(s) 153
TseI GCWGC 4 cut(s) 1316, 1326, 1358, 1459
Tsp45I GTSAC 1 cut(s) 153
TspDTI ATGAA 2 cut(s) 347, 1020
TspGWI ACGGA 3 cut(s) 871, 904, 1207
TspRI CASTG 3 cut(s) 465, 1236, 1477
VneI GTGCAC 1 cut(s) 715
XapI RAATTY 1 cut(s) 530
XceI RCATGY 1 cut(s) 1244
XhoI CTCGAG 1 cut(s) 624
XmiI GTMKAC 3 cut(s) 821, 1351, 1490
XmnI GAANNNNTTC 2 cut(s) 113, 386
XspI CTAG 1 cut(s) 447
Zsp2I ATGCAT 1 cut(s) 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.