Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0001456

Physical Location & Seq

Genomic Coordinates Forward (+)

11797263 .. 11800384

Gene Structure 3122 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00009350.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1803 bp

ATGGCCTCCTCTTCCGGGAGCTTAGACACCTCCGCAAATTCTCACCCGAGCAACTTCACCTTCTCCACCCACCCATTCATGACCACCTCCTTCTCCGACCTCCTATCCTCCGGCGCCGACACCACAAACCCAATAGCCACCGCAACCCGAAACTCCGGGTACGGGTTATCGGATCGTATTGCGGAGCGAACCGGGTCGGGTGTCCCCAAGTTCAAGTCCCTCCCGCCTCCCCAGCTCCCCATTTCGCCTCCCTCTGTTGTGTCTCCCTCTTCCTACTTTGCAATCCCACCTGGGATGAGCCCCGCCGAGCTTCTCGACTCTCCTGTGCTTCTCAGCTCTTCCAACATTCTTCCATCTCCAACTACTGGGAGTTTTGCAGCTCAAGCCTTTAATTGGAGGACCAATTCTGGTTCTGGGCCCCCTCCAACTTCATCATCAGCAATTTTCCAATCCTCAAACAGCTCAATTCAGACTGCACAAGACCAGGCATGGAATAACTATCTAGGACCTCCCAAACAAGATAACTTTTCTTCAGGGAAGAGTATGGTGAAACCTGAGTATGGTTCTTCAGTGCAGAGCTTCTCTTCTGAAATGGGGACTAATATGCAGAGCAATAACAATGCTCAGCCCAACAGCAGTGGTGGTTTCCAATCAGATCAGTACAGCAACTACACTAACAACAACAACAACAACAGCAACAACAACCAGCTGCAATCCCATCAGTGCTTGAACAGGAGATCAGATGATGGGTTCAATTGGAGGAAATATGGCCAGAAACAAGTGAAGGGAAGCGAAAATCCGCGGAGCTATTACAAGTGTACTTATCCGAATTGCCCAACTAAGAAAAAGGTTGAGAGGTCGTTGGATGGACAAATCACCGAGATAGTTTACAAGGGTAATCATAACCATCCCAAGCCACAGAATACTAGAAGGTCGGGTTCATCAGCGGCTTCTTCCCATGCAATTCAGGCTTCCAATCACACCACCAATGACATCCCGGATCAGTCTTTCGGCTCTCATGGTAATTCACAAATGGATAACTCCATTGGAACACCGGAGAATTCATCCAATTTTTCAAATGGGGATGATGAGTTTGATCAGAGCTCTCAGAGAAGCAAGTCAGGAGGAGGGGATGAGTATGATGAGGATGAGCCTAATGCCAAAAGATGGAAGAAAGAAGGTGACAATGAATGTATTTCAGCACCTGGGAGTAGGACGGTAAGAGAACCAAGAGTTGTAGTTCAAACAACAAGTGACATTGATATTCTTGATGATGGCTACAGGTGGAGGAAGTATGGTCAGAAAGTAGTAAAGGGCAACCCAAATCCAAGGAGCTACTACAAGTGCACAAATCCAGGCTGTCCAGTGAGGAAGCACGTAGAGCGAGCTTCACATGATATTAGAGCAGTGATCACAACCTATGAAGGGAAGCACAACCACGATGTTCCTGCAGCCCGTGGCAGTGGTTCTGCCAACAGGCCTTTACCAAGCCACAATACCAACAACAACAACATGAACAACAACCACAATGTACCCACAGCAATGAGGCCTATGACCCATCACAGTACTAACAATGCTACTAACAACAACCCTCTAGACAATCTAAGAAGGCTACCGACATCAGAAGGGCAAGCACCCTTTACTCTCGAGATGTTGCAGAGCCCAGCAAGTTATGGATTTGCAGGGTTTGATAACCCCATGGGGTCATACATGAACCAAACACAGCTCAATGACAACAACATGTTTTCTAAAACCAAGGACGAGCCAAGAGATGACGCATTTTTTGAATCATTGCTGTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006725 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009409 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009700 GO:0009889 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010035 GO:0010120 GO:0010200 GO:0010243 GO:0010286 GO:0010468 GO:0010506 GO:0010508 GO:0010556 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019748 GO:0031323 GO:0031325 GO:0031326 GO:0031329 GO:0031331 GO:0033554 GO:0034605 GO:0034641 GO:0042221 GO:0042430 GO:0042435 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044424 GO:0044464 GO:0044550 GO:0046217 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0065007 GO:0070370 GO:0071704 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

WRKY

Protein Analysis

600

Amino Acids

65.51

Weight (kDa)

7.26

Isoelectric Point (pI)

52.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	248 - 304	1.5e-26	WRKY DNA -binding domain
WRKY	PF03106	424 - 481	3.2e-26	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001456)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07100 AT5G07100 AT5G07100 AT5G07100 AT5G07100
fragaria_vesca	FvH4_3g39850 FvH4_3g39850 FvH4_3g39850 FvH4_3g39850 FvH4_3g39850 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510
malus_domestica	MD03G1057400.v1.1 MD04G1167700.v1.1 MD06G1115200.v1.1 MD11G1059400.v1.1 MD12G1181000.v1.1
prunus_persica	Prupe.6G046900_v2.0.a1 Prupe.6G286000_v2.0.a1
pyrus_communis	pycom03g04580 pycom04g14890 pycom06g10790 pycom11g04930 pycom12g17060
rosa_chinensis	RchiOBHm_Chr3g0461481 RchiOBHm_Chr5g0071811
rosa_laevigata	RLG00000024931 RLG00000036250
rosa_multiflora	Rmu_sc0002677.1_g000005 Rmu_ssc0000127.1_g000054
rosa_roxburghii	Rroxscaffold_1G00009350 Rroxscaffold_6G00418250 Rroxscaffold_6G00418580
rosa_rugosa	Rorug03G0051000 Rorug03G0051100.1 Rorug05G0414700
rosa_samantha	Rh3AG108800 Rh3BG112700 Rh3CG114200 Rh3DG114300 Rh5AG471000 Rh5BG490700 Rh5CG514700 Rh5DG502000
rosa_wichuraiana	Rw3G009250 Rw5G043770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	113
AccB7I	CCANNNNNTGG	2 cut(s)	365, 1167
AccII	CGCG	1 cut(s)	802
AciI	CCGC	8 cut(s)	33, 141, 182, 224, 303, 800, 802, 947
AclWI	GGATC	2 cut(s)	180, 1008
AcoI	YGGCCR	1 cut(s)	769
AcsI	RAATTY	2 cut(s)	37, 1060
AcuI	CTGAAG	2 cut(s)	516, 552
AcyI	GRCGYC	1 cut(s)	114
AfaI	GTAC	5 cut(s)	161, 662, 820, 1533, 1567
AfiI	CCNNNNNNNGG	7 cut(s)	15, 162, 365, 393, 560, 1167, 1425
AflIII	ACRYGT	1 cut(s)	1740
AgsI	TTSAA	6 cut(s)	214, 730, 754, 1077, 1244, 1787
AjnI	CCWGG	4 cut(s)	289, 483, 1204, 1354
Alw21I	GWGCWC	2 cut(s)	1106, 1349
Alw26I	GTCTC	1 cut(s)	267
Alw44I	GTGCAC	1 cut(s)	1345
AlwI	GGATC	2 cut(s)	180, 1008
AlwNI	CAGNNNCTG	1 cut(s)	1205
Ama87I	CYCGRG	2 cut(s)	46, 1646
AoxI	GGCC	5 cut(s)	3, 416, 769, 1478, 1547
ApaI	GGGCCC	1 cut(s)	420
ApaLI	GTGCAC	1 cut(s)	1345
ApeKI	GCWGC	3 cut(s)	377, 709, 1451
ApoI	RAATTY	2 cut(s)	37, 1060
ArsI	GACNNNNNNTTYG	2 cut(s)	1766, 1798
AspLEI	GCGC	1 cut(s)	116
AspS9I	GGNCC	4 cut(s)	399, 416, 417, 506
AsuC2I	CCSGG	4 cut(s)	16, 157, 193, 998
AsuHPI	GGTGA	5 cut(s)	35, 49, 559, 868, 1193
AvaI	CYCGRG	2 cut(s)	46, 1646
AvaII	GGWCC	2 cut(s)	399, 506
BaeGI	GKGCMC	2 cut(s)	420, 1349
BalI	TGGCCA	1 cut(s)	771
BanI	GGYRCC	1 cut(s)	113
BanII	GRGCYC	4 cut(s)	302, 420, 1106, 1664
Bbv12I	GWGCWC	2 cut(s)	1106, 1349
BbvI	GCAGC	3 cut(s)	389, 696, 1463
BccI	CCATC	8 cut(s)	361, 726, 740, 860, 915, 1161, 1268, 1566
BcgI	CGANNNNNNTGC	2 cut(s)	1430, 1464
BciT130I	CCWGG	4 cut(s)	291, 485, 1206, 1356
BclI	TGATCA	2 cut(s)	1096, 1410
BcnI	CCSGG	4 cut(s)	16, 157, 193, 998
BcoDI	GTCTC	1 cut(s)	267
BfaI	CTAG	3 cut(s)	503, 927, 1595
BfmI	CTRYAG	2 cut(s)	1279, 1449
BfoI	RGCGCY	1 cut(s)	117
BisI	GCNGC	4 cut(s)	378, 710, 948, 1452
BlpI	GCTNAGC	1 cut(s)	624
BlsI	GCNGC	4 cut(s)	379, 711, 949, 1453
BmcAI	AGTACT	1 cut(s)	1567
Bme1390I	CCNGG	8 cut(s)	16, 157, 193, 291, 485, 998, 1206, 1356
Bme18I	GGWCC	2 cut(s)	399, 506
BmeT110I	CYCGRG	2 cut(s)	46, 1646
BmgT120I	GGNCC	4 cut(s)	399, 416, 417, 506
BmiI	GGNNCC	3 cut(s)	115, 418, 419
BmrFI	CCNGG	8 cut(s)	16, 157, 193, 291, 485, 998, 1206, 1356
BmrI	ACTGGG	1 cut(s)	375
BmuI	ACTGGG	1 cut(s)	375
Bpu1102I	GCTNAGC	1 cut(s)	624
BpuEI	CTTGAG	1 cut(s)	366
BpuMI	CCSGG	4 cut(s)	16, 157, 193, 998
BsaAI	YACGTR	1 cut(s)	1378
BsaHI	GRCGYC	1 cut(s)	114
BsaJI	CCNNGG	7 cut(s)	290, 800, 1205, 1328, 1456, 1698, 1755
BsaWI	WCCGGW	1 cut(s)	1054
Bsc4I	CCNNNNNNNGG	7 cut(s)	15, 162, 365, 393, 560, 1167, 1425
Bse1I	ACTGG	2 cut(s)	370, 1364
Bse3DI	GCAATG	2 cut(s)	1548, 1790
BseBI	CCWGG	4 cut(s)	291, 485, 1206, 1356
BseDI	CCNNGG	7 cut(s)	290, 800, 1205, 1328, 1456, 1698, 1755
BseGI	GGATG	8 cut(s)	300, 871, 907, 993, 1064, 1090, 1138, 1153
BseLI	CCNNNNNNNGG	7 cut(s)	15, 162, 365, 393, 560, 1167, 1425
BseMI	GCAATG	2 cut(s)	1548, 1790
BseMII	CTCAG	4 cut(s)	346, 546, 638, 1121
BseNI	ACTGG	2 cut(s)	370, 1364
BseRI	GAGGAG	1 cut(s)	1140
BseSI	GKGCMC	2 cut(s)	420, 1349
BseXI	GCAGC	3 cut(s)	389, 696, 1463
BseYI	CCCAGC	2 cut(s)	231, 1663
BsgI	GTGCAG	2 cut(s)	459, 593
Bsh1236I	CGCG	1 cut(s)	802
BshFI	GGCC	5 cut(s)	5, 418, 771, 1480, 1549
BshNI	GGYRCC	1 cut(s)	113
BsiHKAI	GWGCWC	2 cut(s)	1106, 1349
BsiHKCI	CYCGRG	2 cut(s)	46, 1646
BsiSI	CCGG	6 cut(s)	15, 111, 156, 192, 998, 1055
BslFI	GGGAC	3 cut(s)	188, 202, 610
BslI	CCNNNNNNNGG	7 cut(s)	15, 162, 365, 393, 560, 1167, 1425
BsmAI	GTCTC	1 cut(s)	267
BsmFI	GGGAC	3 cut(s)	188, 202, 610
BsnI	GGCC	5 cut(s)	5, 418, 771, 1480, 1549
BsoBI	CYCGRG	2 cut(s)	46, 1646
Bsp120I	GGGCCC	1 cut(s)	416
Bsp1286I	GDGCHC	5 cut(s)	302, 420, 1106, 1349, 1664
Bsp143I	GATC	6 cut(s)	172, 655, 737, 1000, 1096, 1410
Bsp1720I	GCTNAGC	1 cut(s)	624
Bsp19I	CCATGG	1 cut(s)	1698
BspACI	CCGC	8 cut(s)	33, 141, 182, 224, 303, 800, 802, 947
BspANI	GGCC	5 cut(s)	5, 418, 771, 1480, 1549
BspCNI	CTCAG	4 cut(s)	345, 547, 637, 1120
BspFNI	CGCG	1 cut(s)	802
BspHI	TCATGA	1 cut(s)	78
BspLI	GGNNCC	3 cut(s)	115, 418, 419
BspMAI	CTGCAG	1 cut(s)	1453
BspPI	GGATC	2 cut(s)	180, 1008
BspQI	GCTCTTC	1 cut(s)	343
BspT107I	GGYRCC	1 cut(s)	113
BsrDI	GCAATG	2 cut(s)	1548, 1790
BsrI	ACTGG	2 cut(s)	370, 1364
BssECI	CCNNGG	7 cut(s)	290, 800, 1205, 1328, 1456, 1698, 1755
BssMI	GATC	6 cut(s)	172, 655, 737, 1000, 1096, 1410
BssNI	GRCGYC	1 cut(s)	114
BssT1I	CCWWGG	3 cut(s)	1328, 1698, 1755
Bst2UI	CCWGG	4 cut(s)	291, 485, 1206, 1356
Bst4CI	ACNGT	2 cut(s)	1219, 1565
Bst6I	CTCTTC	5 cut(s)	16, 274, 343, 533, 589
BstACI	GRCGYC	1 cut(s)	114
BstBAI	YACGTR	1 cut(s)	1378
BstC8I	GCNNGC	2 cut(s)	1386, 1632
BstDEI	CTNAG	7 cut(s)	22, 332, 555, 624, 840, 1107, 1604
BstDSI	CCRYGG	3 cut(s)	800, 1456, 1698
BstF5I	GGATG	8 cut(s)	300, 871, 907, 993, 1064, 1090, 1138, 1153
BstFNI	CGCG	1 cut(s)	802
BstH2I	RGCGCY	1 cut(s)	117
BstHHI	GCGC	1 cut(s)	116
BstKTI	GATC	6 cut(s)	175, 658, 740, 1003, 1099, 1413
BstMAI	GTCTC	1 cut(s)	267
BstMBI	GATC	6 cut(s)	172, 655, 737, 1000, 1096, 1410
BstMWI	GCNNNNNNNGC	4 cut(s)	232, 383, 968, 1381
BstNI	CCWGG	4 cut(s)	291, 485, 1206, 1356
BstNSI	RCATGY	1 cut(s)	1744
BstSCI	CCNGG	8 cut(s)	14, 155, 191, 289, 483, 996, 1204, 1354
BstSFI	CTRYAG	2 cut(s)	1279, 1449
BstSLI	GKGCMC	2 cut(s)	420, 1349
BstUI	CGCG	1 cut(s)	802
BstV1I	GCAGC	3 cut(s)	389, 696, 1463
BsuRI	GGCC	5 cut(s)	5, 418, 771, 1480, 1549
BtgI	CCRYGG	3 cut(s)	800, 1456, 1698
BtsCI	GGATG	8 cut(s)	300, 871, 907, 993, 1064, 1090, 1138, 1153
BtsI	GCAGTG	3 cut(s)	643, 1413, 1468
BtsIMutI	CAGTG	6 cut(s)	576, 643, 728, 1371, 1413, 1468
Cac8I	GCNNGC	2 cut(s)	1386, 1632
CaiI	CAGNNNCTG	1 cut(s)	1205
CciI	TCATGA	1 cut(s)	78
CfoI	GCGC	1 cut(s)	116
Cfr13I	GGNCC	4 cut(s)	399, 416, 417, 506
Cfr42I	CCGCGG	1 cut(s)	803
CseI	GACGC	1 cut(s)	1784
Csp6I	GTAC	5 cut(s)	160, 661, 819, 1532, 1566
CspCI	CAANNNNNGTGG	2 cut(s)	619, 654
CviAII	CATG	9 cut(s)	79, 489, 959, 1019, 1394, 1513, 1699, 1711, 1741
CviQI	GTAC	5 cut(s)	160, 661, 819, 1532, 1566
DdeI	CTNAG	7 cut(s)	22, 332, 555, 624, 840, 1107, 1604
DinI	GGCGCC	1 cut(s)	115
DpnI	GATC	6 cut(s)	174, 657, 739, 1002, 1098, 1412
DpnII	GATC	6 cut(s)	172, 655, 737, 1000, 1096, 1410
EaeI	YGGCCR	1 cut(s)	769
Eam1104I	CTCTTC	5 cut(s)	16, 274, 343, 533, 589
EarI	CTCTTC	5 cut(s)	16, 274, 343, 533, 589
Ecl136II	GAGCTC	1 cut(s)	1104
Eco130I	CCWWGG	3 cut(s)	1328, 1698, 1755
Eco147I	AGGCCT	2 cut(s)	1480, 1549
Eco24I	GRGCYC	4 cut(s)	302, 420, 1106, 1664
Eco47I	GGWCC	2 cut(s)	399, 506
Eco53kI	GAGCTC	1 cut(s)	1104
Eco57I	CTGAAG	2 cut(s)	516, 552
Eco88I	CYCGRG	2 cut(s)	46, 1646
EcoICRI	GAGCTC	1 cut(s)	1104
EcoO109I	RGGNCCY	2 cut(s)	417, 506
EcoRI	GAATTC	1 cut(s)	1060
EcoRII	CCWGG	4 cut(s)	289, 483, 1204, 1354
EcoT14I	CCWWGG	3 cut(s)	1328, 1698, 1755
EcoT38I	GRGCYC	4 cut(s)	302, 420, 1106, 1664
EgeI	GGCGCC	1 cut(s)	115
EheI	GGCGCC	1 cut(s)	115
ErhI	CCWWGG	3 cut(s)	1328, 1698, 1755
FaeI	CATG	9 cut(s)	82, 492, 962, 1022, 1397, 1516, 1702, 1714, 1744
FaqI	GGGAC	3 cut(s)	188, 202, 610
FatI	CATG	9 cut(s)	78, 488, 958, 1018, 1393, 1512, 1698, 1710, 1740
FauI	CCCGC	2 cut(s)	231, 310
FbaI	TGATCA	2 cut(s)	1096, 1410
Fnu4HI	GCNGC	4 cut(s)	378, 710, 948, 1452
FokI	GGATG	8 cut(s)	307, 878, 894, 980, 1051, 1097, 1145, 1160
FriOI	GRGCYC	4 cut(s)	302, 420, 1106, 1664
Fsp4HI	GCNGC	4 cut(s)	378, 710, 948, 1452
FspBI	CTAG	3 cut(s)	503, 927, 1595
GlaI	GCGC	1 cut(s)	115
GluI	GCNGC	4 cut(s)	378, 710, 948, 1452
GsaI	CCCAGC	2 cut(s)	235, 1667
HaeII	RGCGCY	1 cut(s)	117
HaeIII	GGCC	5 cut(s)	5, 418, 771, 1480, 1549
HapII	CCGG	6 cut(s)	15, 111, 156, 192, 998, 1055
HgaI	GACGC	1 cut(s)	1784
HhaI	GCGC	1 cut(s)	116
Hin1I	GRCGYC	1 cut(s)	114
Hin1II	CATG	9 cut(s)	82, 492, 962, 1022, 1397, 1516, 1702, 1714, 1744
Hin6I	GCGC	1 cut(s)	114
HinP1I	GCGC	1 cut(s)	114
HinfI	GANTC	2 cut(s)	317, 1787
HpaII	CCGG	6 cut(s)	15, 111, 156, 192, 998, 1055
HphI	GGTGA	5 cut(s)	35, 49, 559, 868, 1193
Hpy166II	GTNNAC	3 cut(s)	819, 889, 1347
Hpy188III	TCNNGA	7 cut(s)	79, 314, 1122, 1268, 1595, 1646, 1648
Hpy8I	GTNNAC	3 cut(s)	819, 889, 1347
HpyAV	CCTTC	8 cut(s)	70, 100, 778, 924, 1172, 1418, 1602, 1619
HpyCH4III	ACNGT	2 cut(s)	1219, 1565
HpyCH4IV	ACGT	1 cut(s)	1377
HpyF10VI	GCNNNNNNNGC	4 cut(s)	232, 383, 968, 1381
HpyF3I	CTNAG	7 cut(s)	22, 332, 555, 624, 840, 1107, 1604
HpySE526I	ACGT	1 cut(s)	1377
Hsp92I	GRCGYC	1 cut(s)	114
Hsp92II	CATG	9 cut(s)	82, 492, 962, 1022, 1397, 1516, 1702, 1714, 1744
HspAI	GCGC	1 cut(s)	114
KasI	GGCGCC	1 cut(s)	113
Ksp22I	TGATCA	2 cut(s)	1096, 1410
KspI	CCGCGG	1 cut(s)	803
Kzo9I	GATC	6 cut(s)	172, 655, 737, 1000, 1096, 1410
LguI	GCTCTTC	1 cut(s)	343
LmnI	GCTCC	5 cut(s)	18, 184, 240, 804, 1332
Lsp1109I	GCAGC	3 cut(s)	389, 696, 1463
MaeI	CTAG	3 cut(s)	503, 927, 1595
MaeII	ACGT	1 cut(s)	1377
MaeIII	GTNAC	2 cut(s)	1181, 1253
MalI	GATC	6 cut(s)	174, 657, 739, 1002, 1098, 1412
MboI	GATC	6 cut(s)	172, 655, 737, 1000, 1096, 1410
MfeI	CAATTG	1 cut(s)	754
MhlI	GDGCHC	5 cut(s)	302, 420, 1106, 1349, 1664
MlsI	TGGCCA	1 cut(s)	771
MluNI	TGGCCA	1 cut(s)	771
Mly113I	GGCGCC	1 cut(s)	114
MlyI	GAGTC	1 cut(s)	311
MmeI	TCCRAC	5 cut(s)	120, 366, 383, 449, 843
Mox20I	TGGCCA	1 cut(s)	771
MscI	TGGCCA	1 cut(s)	771
MseI	TTAA	1 cut(s)	390
MslI	CAYNNNNRTG	2 cut(s)	1541, 1795
Msp20I	TGGCCA	1 cut(s)	771
MspA1I	CMGCKG	3 cut(s)	709, 802, 947
MspI	CCGG	6 cut(s)	15, 111, 156, 192, 998, 1055
MspR9I	CCNGG	8 cut(s)	16, 157, 193, 291, 485, 998, 1206, 1356
MunI	CAATTG	1 cut(s)	754
MvaI	CCWGG	4 cut(s)	291, 485, 1206, 1356
MvnI	CGCG	1 cut(s)	802
MwoI	GCNNNNNNNGC	4 cut(s)	232, 383, 968, 1381
NarI	GGCGCC	1 cut(s)	114
NciI	CCSGG	4 cut(s)	16, 157, 193, 998
NcoI	CCATGG	1 cut(s)	1698
NdeII	GATC	6 cut(s)	172, 655, 737, 1000, 1096, 1410
NlaIII	CATG	9 cut(s)	82, 492, 962, 1022, 1397, 1516, 1702, 1714, 1744
NlaIV	GGNNCC	3 cut(s)	115, 418, 419
NmeAIII	GCCGAG	1 cut(s)	331
NmuCI	GTSAC	2 cut(s)	1181, 1253
NspI	RCATGY	1 cut(s)	1744
PaeR7I	CTCGAG	1 cut(s)	1646
PagI	TCATGA	1 cut(s)	78
PceI	AGGCCT	2 cut(s)	1480, 1549
PciI	ACATGT	1 cut(s)	1740
PciSI	GCTCTTC	1 cut(s)	343
PfeI	GAWTC	1 cut(s)	1787
PflMI	CCANNNNNTGG	2 cut(s)	365, 1167
PfoI	TCCNGGA	2 cut(s)	14, 996
PkrI	GCNGC	4 cut(s)	379, 711, 949, 1453
PleI	GAGTC	1 cut(s)	311
PluTI	GGCGCC	1 cut(s)	117
PpsI	GAGTC	1 cut(s)	311
Ppu21I	YACGTR	1 cut(s)	1378
PpuMI	RGGWCCY	1 cut(s)	506
PscI	ACATGT	1 cut(s)	1740
Psp124BI	GAGCTC	1 cut(s)	1106
Psp5II	RGGWCCY	1 cut(s)	506
Psp6I	CCWGG	4 cut(s)	289, 483, 1204, 1354
PspFI	CCCAGC	2 cut(s)	231, 1663
PspGI	CCWGG	4 cut(s)	289, 483, 1204, 1354
PspN4I	GGNNCC	3 cut(s)	115, 418, 419
PspOMI	GGGCCC	1 cut(s)	416
PspPI	GGNCC	4 cut(s)	399, 416, 417, 506
PspPPI	RGGWCCY	1 cut(s)	506
PstI	CTGCAG	1 cut(s)	1453
PstNI	CAGNNNCTG	1 cut(s)	1205
PvuII	CAGCTG	1 cut(s)	709
RsaI	GTAC	5 cut(s)	161, 662, 820, 1533, 1567
RsaNI	GTAC	5 cut(s)	160, 661, 819, 1532, 1566
RseI	CAYNNNNRTG	2 cut(s)	1541, 1795
SacI	GAGCTC	1 cut(s)	1106
SacII	CCGCGG	1 cut(s)	803
SapI	GCTCTTC	1 cut(s)	343
SaqAI	TTAA	1 cut(s)	390
SatI	GCNGC	4 cut(s)	378, 710, 948, 1452
Sau3AI	GATC	6 cut(s)	172, 655, 737, 1000, 1096, 1410
Sau96I	GGNCC	4 cut(s)	399, 416, 417, 506
ScaI	AGTACT	1 cut(s)	1567
SchI	GAGTC	1 cut(s)	311
ScrFI	CCNGG	8 cut(s)	16, 157, 193, 291, 485, 998, 1206, 1356
SduI	GDGCHC	5 cut(s)	302, 420, 1106, 1349, 1664
SfcI	CTRYAG	2 cut(s)	1279, 1449
SfoI	GGCGCC	1 cut(s)	115
Sfr274I	CTCGAG	1 cut(s)	1646
Sfr303I	CCGCGG	1 cut(s)	803
SgrBI	CCGCGG	1 cut(s)	803
SinI	GGWCC	2 cut(s)	399, 506
SlaI	CTCGAG	1 cut(s)	1646
SmiMI	CAYNNNNRTG	2 cut(s)	1541, 1795
SmlI	CTYRAG	2 cut(s)	381, 1646
SmoI	CTYRAG	2 cut(s)	381, 1646
SseBI	AGGCCT	2 cut(s)	1480, 1549
SsiI	CCGC	8 cut(s)	33, 141, 182, 224, 303, 800, 802, 947
SspDI	GGCGCC	1 cut(s)	113
SspMI	CTAG	3 cut(s)	503, 927, 1595
SstI	GAGCTC	1 cut(s)	1106
StuI	AGGCCT	2 cut(s)	1480, 1549
StyD4I	CCNGG	8 cut(s)	14, 155, 191, 289, 483, 996, 1204, 1354
StyI	CCWWGG	3 cut(s)	1328, 1698, 1755
TaaI	ACNGT	2 cut(s)	1219, 1565
TaiI	ACGT	1 cut(s)	1380
TaqI	TCGA	2 cut(s)	315, 1647
TatI	WGTACW	3 cut(s)	660, 818, 1565
TauI	GCSGC	1 cut(s)	950
TfiI	GAWTC	1 cut(s)	1787
Tru1I	TTAA	1 cut(s)	390
Tru9I	TTAA	1 cut(s)	390
TscAI	CASTG	6 cut(s)	576, 643, 728, 1371, 1413, 1468
TseFI	GTSAC	2 cut(s)	1181, 1253
TseI	GCWGC	3 cut(s)	377, 709, 1451
Tsp45I	GTSAC	2 cut(s)	1181, 1253
TspDTI	ATGAA	8 cut(s)	67, 420, 930, 1053, 1203, 1437, 1529, 1727
TspRI	CASTG	6 cut(s)	576, 643, 728, 1371, 1413, 1468
Van91I	CCANNNNNTGG	2 cut(s)	365, 1167
VneI	GTGCAC	1 cut(s)	1345
VpaK11BI	GGWCC	2 cut(s)	399, 506
XapI	RAATTY	2 cut(s)	37, 1060
XbaI	TCTAGA	1 cut(s)	1594
XceI	RCATGY	1 cut(s)	1744
XhoI	CTCGAG	1 cut(s)	1646
XspI	CTAG	3 cut(s)	503, 927, 1595
ZrmI	AGTACT	1 cut(s)	1567

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.