MD04G1167700.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001456
Physical Location & Seq
Reverse (-)
25792146 .. 25794577
2432 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1167700.v1.1.491

Sequence Viewer

Length: 1563 bp
ATGACTTCTTCCTTCACGAACCTCCTCACAAGCAACATGGAGAACAACGGGATCGGACAGGACAGGACTAATTGGGGGGGACAATTCTCGGACTATTCGTCTAATCCGAACCGGTTTACGGACAGAAACGGGACTGAAATCCCCAAGTTCAGATCACTCCAGCCTCCTTCTCTGCCTCTCTCTCCTCCCCCTGTCTCCCCTTCTTCTTACTTGGCTTCCACGCCAGCTTTTAGTCCCACCGATTTTTTCAGCTCACCCATGTTCCTCTCCTCCTCAAATAATCTTGAATCTCCAACAACTGGAGCTTTTTCGAGTCAGATTTTCGATTGGGTGAATAATAGTAAGGACACCCAGCAAGGAATTAGGGAAAGCGAGCCGAAAATGTTCTCCGATTTCTCGTTCCAACCGGAATCGCGGCCTGCAACTAATCTTCAATCTGCTTCTAGCATGGTTTCAGTGGAAGAACCATTCAAAAGAGAAAGGCAAGCGTGGGACTTTAGTACAACAAGGCAAGCTGATTCCTCAGCTGAGAAGACAGGAGTCAAGTCGGAATTCGAGCCAATTGAAGCGAATACTCAGAGTAATGGACTTAATGGTGCTCCCAAGTCCGATTACTTGCATTCTACTCAATCTTCGCAATATGGTCGAGAACAAAAATCGGATGATGGGTTCAATTGGAGGAAGTATGGGCAGAAACAAGTGAAGGGTAGCGAAAATCCGCGGAGTTATTACAAGTGCACGTTTCCGAATTGCCCGACAAAGAAGAAGGTTGAGAGATCATTGGATGGACAGATTACTCAAATTGTGTACAAGGGTAGTCACAACCATCCTAAGCCTCAGTCTACAAGAAGATCAAGCTCGAACGCAATTCAAGGTTCTTCGTATGTAATCTCCGATCAATCCGTTCCAACGTTATCCAATCCGAAAGTTGAGTCCATCACACTGCAGGAGGATTCTTCAACCTCAATGGGAGAGGATGAGTTTGAACAAAACTCTCCAATAAGTAATTCAGGAGGAGCTGAAGACGAAAATGAGCCTGAGGCGAAAAGATGGAAGGGAGAAAATGCAAATGATCAAGCCTACGTATCTTCTGGTAGTAGAATTGTAAAAGAACCAAGAATTGTGGTGCAAACAACAAGCGAAATCGATATTCTGGATGACGGGTATAGGTGGAGGAAGTATGGACAGAAAGTAGTGAAGGGAAATCCGAATCCGAGGAGCTACTACAAATGCACATCCGTGGGTTGTCCGGTGAGGAAGCATGTGGAGCGAGCATCGCATGACACAAAGGCGGTGATCACCACGTACGAAGGGAAACACAACCACGATGTTCCCGCAGCTCGTGGGAGCGGCAATTACAGCAATGCAAGTAGACCCGCTGCCGATAATAGCAGCAACAATGTGTCCATGGCTGTGAGGCCCTTGGCATTGCCCAACCATTCTAATTTGAGGTACCTCAACTCTCTTCAGAACACAAGGCAGCCATCAACCACTGAAAGCCAACCGCCGTTTACACTCAAAATGTTGCAGAGTGAAGGAAGTTATGGATTTTCTGAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
WRKY

Protein Analysis

521

Amino Acids

57.71

Weight (kDa)

7.1

Isoelectric Point (pI)

59.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 221 - 277 3e-26 WRKY DNA -binding domain
WRKY PF03106 386 - 443 3.9e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1452
AccB1I GGYRCC 1 cut(s) 1452
AccB7I CCANNNNNTGG 1 cut(s) 299
AccBSI CCGCTC 1 cut(s) 1350
AccI GTMKAC 2 cut(s) 842, 1372
AccII CGCG 2 cut(s) 415, 721
AciI CCGC 8 cut(s) 415, 719, 721, 1292, 1335, 1350, 1377, 1505
AclI AACGTT 1 cut(s) 911
AclWI GGATC 1 cut(s) 59
AcsI RAATTY 1 cut(s) 551
AcuI CTGAAG 2 cut(s) 1041, 1451
AfaI GTAC 4 cut(s) 502, 809, 1307, 1454
AfiI CCNNNNNNNGG 2 cut(s) 118, 299
AgeI ACCGGT 1 cut(s) 111
AgsI TTSAA 8 cut(s) 287, 434, 472, 566, 673, 872, 960, 986
AhdI GACNNNNNGTC 1 cut(s) 97
AjuI GAANNNNNNNTTGG 2 cut(s) 991, 1023
AleI CACNNNNGTG 1 cut(s) 1238
AloI GAACNNNNNNTCC 2 cut(s) 653, 685
AluBI AGCT 9 cut(s) 227, 252, 305, 515, 527, 858, 1019, 1221, 1340
AluI AGCT 9 cut(s) 227, 252, 305, 515, 527, 858, 1019, 1221, 1340
Alw21I GWGCWC 2 cut(s) 601, 740
Alw26I GTCTC 1 cut(s) 199
Alw44I GTGCAC 1 cut(s) 736
AlwI GGATC 1 cut(s) 59
AoxI GGCC 2 cut(s) 416, 1418
ApaLI GTGCAC 1 cut(s) 736
ApeKI GCWGC 4 cut(s) 1337, 1379, 1392, 1480
ApoI RAATTY 1 cut(s) 551
ArsI GACNNNNNNTTYG 2 cut(s) 917, 949
AsiGI ACCGGT 1 cut(s) 111
Asp700I GAANNNNTTC 1 cut(s) 383
Asp718I GGTACC 1 cut(s) 1452
AspS9I GGNCC 1 cut(s) 1419
AsuHPI GGTGA 5 cut(s) 246, 343, 1264, 1291, 1306
AxyI CCTNAGG 1 cut(s) 1038
BaeGI GKGCMC 1 cut(s) 740
BanI GGYRCC 1 cut(s) 1452
BauI CACGAG 1 cut(s) 1341
BbsI GAAGAC 2 cut(s) 539, 1029
Bbv12I GWGCWC 2 cut(s) 601, 740
BbvCI CCTCAGC 1 cut(s) 523
BbvI GCAGC 4 cut(s) 1349, 1366, 1404, 1492
BccI CCATC 6 cut(s) 659, 779, 834, 944, 1044, 1492
BceAI ACGGC 1 cut(s) 1492
BcgI CGANNNNNNTGC 2 cut(s) 626, 660
BclI TGATCA 2 cut(s) 1072, 1296
BcoDI GTCTC 1 cut(s) 199
BfaI CTAG 1 cut(s) 444
BfmI CTRYAG 1 cut(s) 944
BisI GCNGC 6 cut(s) 416, 1338, 1351, 1380, 1393, 1481
BlsI GCNGC 6 cut(s) 417, 1339, 1352, 1381, 1394, 1482
BmeRI GACNNNNNGTC 1 cut(s) 97
BmgT120I GGNCC 1 cut(s) 1419
BmiI GGNNCC 1 cut(s) 1454
BmsI GCATC 1 cut(s) 1283
BoxI GACNNNNGTC 1 cut(s) 539
BpiI GAAGAC 2 cut(s) 539, 1029
BpmI CTGGAG 2 cut(s) 143, 321
Bpu10I CCTNAGC 2 cut(s) 523, 831
Bsa29I ATCGAT 1 cut(s) 1146
BsaAI YACGTR 2 cut(s) 1084, 1305
BsaJI CCNNGG 5 cut(s) 719, 1214, 1239, 1407, 1422
BsaWI WCCGGW 3 cut(s) 111, 406, 1249
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 299
Bse118I RCCGGY 1 cut(s) 111
Bse1I ACTGG 1 cut(s) 304
Bse21I CCTNAGG 1 cut(s) 1038
Bse3DI GCAATG 2 cut(s) 1369, 1427
BseCI ATCGAT 1 cut(s) 1146
BseDI CCNNGG 5 cut(s) 719, 1214, 1239, 1407, 1422
BseGI GGATG 6 cut(s) 667, 790, 826, 982, 1162, 1235
BseLI CCNNNNNNNGG 2 cut(s) 118, 299
BseMI GCAATG 2 cut(s) 1369, 1427
BseMII CTCAG 6 cut(s) 519, 537, 590, 851, 1029, 1545
BseNI ACTGG 1 cut(s) 304
BseRI GAGGAG 6 cut(s) 14, 174, 259, 262, 1029, 1231
BseSI GKGCMC 1 cut(s) 740
BseXI GCAGC 4 cut(s) 1349, 1366, 1404, 1492
BseYI CCCAGC 1 cut(s) 351
Bsh1236I CGCG 2 cut(s) 415, 721
BshFI GGCC 2 cut(s) 418, 1420
BshNI GGYRCC 1 cut(s) 1452
BshTI ACCGGT 1 cut(s) 111
BshVI ATCGAT 1 cut(s) 1146
BsiHKAI GWGCWC 2 cut(s) 601, 740
BsiSI CCGG 3 cut(s) 112, 407, 1250
BsiWI CGTACG 1 cut(s) 1305
BslFI GGGAC 4 cut(s) 93, 145, 219, 506
BslI CCNNNNNNNGG 2 cut(s) 118, 299
BsmAI GTCTC 1 cut(s) 199
BsmFI GGGAC 4 cut(s) 93, 145, 219, 506
BsmI GAATGC 1 cut(s) 619
BsnI GGCC 2 cut(s) 418, 1420
Bsp1286I GDGCHC 2 cut(s) 601, 740
Bsp1407I TGTACA 1 cut(s) 807
Bsp143I GATC 7 cut(s) 51, 152, 776, 851, 895, 1072, 1296
Bsp19I CCATGG 1 cut(s) 1407
BspACI CCGC 8 cut(s) 415, 719, 721, 1292, 1335, 1350, 1377, 1505
BspANI GGCC 2 cut(s) 418, 1420
BspCNI CTCAG 6 cut(s) 520, 536, 589, 850, 1030, 1546
BspDI ATCGAT 1 cut(s) 1146
BspFNI CGCG 2 cut(s) 415, 721
BspLI GGNNCC 1 cut(s) 1454
BspMAI CTGCAG 1 cut(s) 948
BspPI GGATC 1 cut(s) 59
BspT107I GGYRCC 1 cut(s) 1452
BsrBI CCGCTC 1 cut(s) 1350
BsrDI GCAATG 2 cut(s) 1369, 1427
BsrFI RCCGGY 1 cut(s) 111
BsrGI TGTACA 1 cut(s) 807
BsrI ACTGG 1 cut(s) 304
BssAI RCCGGY 1 cut(s) 111
BssECI CCNNGG 5 cut(s) 719, 1214, 1239, 1407, 1422
BssMI GATC 7 cut(s) 51, 152, 776, 851, 895, 1072, 1296
BssSI CACGAG 1 cut(s) 1341
BssT1I CCWWGG 2 cut(s) 1407, 1422
Bst2BI CACGAG 1 cut(s) 1341
Bst6I CTCTTC 1 cut(s) 1470
BstAUI TGTACA 1 cut(s) 807
BstBAI YACGTR 2 cut(s) 1084, 1305
BstC8I GCNNGC 6 cut(s) 225, 374, 420, 486, 513, 1272
BstDEI CTNAG 7 cut(s) 523, 528, 576, 831, 837, 1038, 1554
BstDSI CCRYGG 3 cut(s) 719, 1239, 1407
BstF5I GGATG 6 cut(s) 667, 790, 826, 982, 1162, 1235
BstFNI CGCG 2 cut(s) 415, 721
BstKTI GATC 7 cut(s) 54, 155, 779, 854, 898, 1075, 1299
BstMAI GTCTC 1 cut(s) 199
BstMBI GATC 7 cut(s) 51, 152, 776, 851, 895, 1072, 1296
BstMWI GCNNNNNNNGC 3 cut(s) 1267, 1276, 1359
BstNSI RCATGY 1 cut(s) 1265
BstPAI GACNNNNGTC 1 cut(s) 539
BstSFI CTRYAG 1 cut(s) 944
BstSLI GKGCMC 1 cut(s) 740
BstSNI TACGTA 1 cut(s) 1084
BstUI CGCG 2 cut(s) 415, 721
BstV1I GCAGC 4 cut(s) 1349, 1366, 1404, 1492
BstV2I GAAGAC 2 cut(s) 539, 1029
Bsu15I ATCGAT 1 cut(s) 1146
Bsu36I CCTNAGG 1 cut(s) 1038
BsuRI GGCC 2 cut(s) 418, 1420
BsuTUI ATCGAT 1 cut(s) 1146
BtgI CCRYGG 3 cut(s) 719, 1239, 1407
BtgZI GCGATG 1 cut(s) 1260
BtsCI GGATG 6 cut(s) 667, 790, 826, 982, 1162, 1235
BtsI GCAGTG 1 cut(s) 941
BtsIMutI CAGTG 3 cut(s) 462, 941, 1491
Cac8I GCNNGC 6 cut(s) 225, 374, 420, 486, 513, 1272
Cfr10I RCCGGY 1 cut(s) 111
Cfr13I GGNCC 1 cut(s) 1419
Cfr42I CCGCGG 1 cut(s) 722
ClaI ATCGAT 1 cut(s) 1146
Csp6I GTAC 4 cut(s) 501, 808, 1306, 1453
CspAI ACCGGT 1 cut(s) 111
CspCI CAANNNNNGTGG 2 cut(s) 1104, 1139
CviAII CATG 6 cut(s) 37, 259, 448, 1262, 1280, 1408
CviQI GTAC 4 cut(s) 501, 808, 1306, 1453
DdeI CTNAG 7 cut(s) 523, 528, 576, 831, 837, 1038, 1554
DpnI GATC 7 cut(s) 53, 154, 778, 853, 897, 1074, 1298
DpnII GATC 7 cut(s) 51, 152, 776, 851, 895, 1072, 1296
DriI GACNNNNNGTC 1 cut(s) 97
Eam1104I CTCTTC 1 cut(s) 1470
Eam1105I GACNNNNNGTC 1 cut(s) 97
EarI CTCTTC 1 cut(s) 1470
Eco105I TACGTA 1 cut(s) 1084
Eco130I CCWWGG 2 cut(s) 1407, 1422
Eco57I CTGAAG 2 cut(s) 1041, 1451
Eco81I CCTNAGG 1 cut(s) 1038
EcoO109I RGGNCCY 1 cut(s) 1419
EcoRI GAATTC 1 cut(s) 551
EcoT14I CCWWGG 2 cut(s) 1407, 1422
ErhI CCWWGG 2 cut(s) 1407, 1422
FaeI CATG 6 cut(s) 40, 262, 451, 1265, 1283, 1411
FaqI GGGAC 4 cut(s) 93, 145, 219, 506
FatI CATG 6 cut(s) 36, 258, 447, 1261, 1279, 1407
FauI CCCGC 2 cut(s) 1342, 1384
FbaI TGATCA 2 cut(s) 1072, 1296
FblI GTMKAC 2 cut(s) 842, 1372
Fnu4HI GCNGC 6 cut(s) 416, 1338, 1351, 1380, 1393, 1481
FokI GGATG 6 cut(s) 674, 797, 813, 989, 1169, 1222
Fsp4HI GCNGC 6 cut(s) 416, 1338, 1351, 1380, 1393, 1481
FspBI CTAG 1 cut(s) 444
GluI GCNGC 6 cut(s) 416, 1338, 1351, 1380, 1393, 1481
GsaI CCCAGC 1 cut(s) 355
GsuI CTGGAG 2 cut(s) 143, 321
HaeIII GGCC 2 cut(s) 418, 1420
HapII CCGG 3 cut(s) 112, 407, 1250
Hin1II CATG 6 cut(s) 40, 262, 451, 1265, 1283, 1411
HinfI GANTC 8 cut(s) 287, 313, 410, 518, 540, 932, 953, 1210
HpaII CCGG 3 cut(s) 112, 407, 1250
HphI GGTGA 5 cut(s) 246, 343, 1264, 1291, 1306
Hpy166II GTNNAC 6 cut(s) 117, 738, 808, 843, 1373, 1512
Hpy188III TCNNGA 5 cut(s) 16, 284, 647, 1011, 1154
Hpy8I GTNNAC 6 cut(s) 117, 738, 808, 843, 1373, 1512
HpyAV CCTTC 9 cut(s) 22, 177, 210, 697, 760, 1048, 1192, 1304, 1529
HpyCH4IV ACGT 4 cut(s) 740, 911, 1083, 1304
HpyCH4V TGCA 9 cut(s) 422, 619, 738, 946, 1067, 1129, 1233, 1367, 1528
HpyF10VI GCNNNNNNNGC 3 cut(s) 1267, 1276, 1359
HpyF3I CTNAG 7 cut(s) 523, 528, 576, 831, 837, 1038, 1554
HpySE526I ACGT 4 cut(s) 740, 911, 1083, 1304
Hsp92II CATG 6 cut(s) 40, 262, 451, 1265, 1283, 1411
KpnI GGTACC 1 cut(s) 1456
Ksp22I TGATCA 2 cut(s) 1072, 1296
KspI CCGCGG 1 cut(s) 722
Kzo9I GATC 7 cut(s) 51, 152, 776, 851, 895, 1072, 1296
LmnI GCTCC 6 cut(s) 302, 604, 1016, 1218, 1267, 1347
Lsp1109I GCAGC 4 cut(s) 1349, 1366, 1404, 1492
LweI GCATC 1 cut(s) 1283
MaeI CTAG 1 cut(s) 444
MaeII ACGT 4 cut(s) 740, 911, 1083, 1304
MaeIII GTNAC 1 cut(s) 818
MalI GATC 7 cut(s) 53, 154, 778, 853, 897, 1074, 1298
MbiI CCGCTC 1 cut(s) 1350
MboI GATC 7 cut(s) 51, 152, 776, 851, 895, 1072, 1296
MfeI CAATTG 2 cut(s) 561, 673
MhlI GDGCHC 2 cut(s) 601, 740
MlyI GAGTC 3 cut(s) 322, 549, 941
MmeI TCCRAC 4 cut(s) 317, 427, 528, 932
MroXI GAANNNNTTC 1 cut(s) 383
MseI TTAA 1 cut(s) 591
MslI CAYNNNNRTG 2 cut(s) 1238, 1412
MspA1I CMGCKG 3 cut(s) 527, 721, 1379
MspI CCGG 3 cut(s) 112, 407, 1250
MunI CAATTG 2 cut(s) 561, 673
Mva1269I GAATGC 1 cut(s) 619
MvnI CGCG 2 cut(s) 415, 721
MwoI GCNNNNNNNGC 3 cut(s) 1267, 1276, 1359
NcoI CCATGG 1 cut(s) 1407
NdeII GATC 7 cut(s) 51, 152, 776, 851, 895, 1072, 1296
NlaIII CATG 6 cut(s) 40, 262, 451, 1265, 1283, 1411
NlaIV GGNNCC 1 cut(s) 1454
NmuCI GTSAC 1 cut(s) 818
NspI RCATGY 1 cut(s) 1265
OliI CACNNNNGTG 1 cut(s) 1238
PcsI WCGNNNNNNNCGW 2 cut(s) 95, 104
PctI GAATGC 1 cut(s) 619
PdmI GAANNNNTTC 1 cut(s) 383
PfeI GAWTC 5 cut(s) 287, 410, 518, 953, 1210
Pfl23II CGTACG 1 cut(s) 1305
PflMI CCANNNNNTGG 1 cut(s) 299
PinAI ACCGGT 1 cut(s) 111
PkrI GCNGC 6 cut(s) 417, 1339, 1352, 1381, 1394, 1482
PleI GAGTC 3 cut(s) 321, 548, 940
PpsI GAGTC 3 cut(s) 321, 548, 940
Ppu21I YACGTR 2 cut(s) 1084, 1305
PshAI GACNNNNGTC 1 cut(s) 539
Psp1406I AACGTT 1 cut(s) 911
PspFI CCCAGC 1 cut(s) 351
PspLI CGTACG 1 cut(s) 1305
PspN4I GGNNCC 1 cut(s) 1454
PspPI GGNCC 1 cut(s) 1419
PstI CTGCAG 1 cut(s) 948
PvuII CAGCTG 1 cut(s) 527
RsaI GTAC 4 cut(s) 502, 809, 1307, 1454
RsaNI GTAC 4 cut(s) 501, 808, 1306, 1453
RseI CAYNNNNRTG 2 cut(s) 1238, 1412
SacII CCGCGG 1 cut(s) 722
SaqAI TTAA 1 cut(s) 591
SatI GCNGC 6 cut(s) 416, 1338, 1351, 1380, 1393, 1481
Sau3AI GATC 7 cut(s) 51, 152, 776, 851, 895, 1072, 1296
Sau96I GGNCC 1 cut(s) 1419
SchI GAGTC 3 cut(s) 322, 549, 941
SduI GDGCHC 2 cut(s) 601, 740
SfaNI GCATC 1 cut(s) 1283
SfcI CTRYAG 1 cut(s) 944
Sfr303I CCGCGG 1 cut(s) 722
SgrBI CCGCGG 1 cut(s) 722
SmiMI CAYNNNNRTG 2 cut(s) 1238, 1412
SnaBI TACGTA 1 cut(s) 1084
SsiI CCGC 8 cut(s) 415, 719, 721, 1292, 1335, 1350, 1377, 1505
SspMI CTAG 1 cut(s) 444
StyI CCWWGG 2 cut(s) 1407, 1422
TaiI ACGT 4 cut(s) 743, 914, 1086, 1307
TaqI TCGA 6 cut(s) 311, 324, 555, 646, 860, 1146
TatI WGTACW 2 cut(s) 500, 807
TauI GCSGC 2 cut(s) 418, 1353
TfiI GAWTC 5 cut(s) 287, 410, 518, 953, 1210
Tru1I TTAA 1 cut(s) 591
Tru9I TTAA 1 cut(s) 591
TscAI CASTG 3 cut(s) 462, 948, 1498
TseFI GTSAC 1 cut(s) 818
TseI GCWGC 4 cut(s) 1337, 1379, 1392, 1480
Tsp45I GTSAC 1 cut(s) 818
TspGWI ACGGA 3 cut(s) 134, 892, 1228
TspRI CASTG 3 cut(s) 462, 948, 1498
Van91I CCANNNNNTGG 1 cut(s) 299
VneI GTGCAC 1 cut(s) 736
XapI RAATTY 1 cut(s) 551
XceI RCATGY 1 cut(s) 1265
XmiI GTMKAC 2 cut(s) 842, 1372
XmnI GAANNNNTTC 1 cut(s) 383
XspI CTAG 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.