RLG00000024931
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001456
Physical Location & Seq
Reverse (-)
40421433 .. 40423384
1952 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024931

Sequence Viewer

Length: 1545 bp
ATGACTTCTTCCTTCACTCACCTCCTCACAAGCAACATGAACATGAACACGAACAATTTTGACCAGGACCGAACCGGCAGCTGGAGCTGGGGACTCTCCGACCGTTCAACGGAAGCCGAAATCCCCAAGTTCAAGTCACTCCAGCCGCCTTCTCTCCCTCTCTCTCCTCCTCCTGCTTCCCCTTCTTCTTACTTGGTTAACACCCCTGGTTTTAGTCCTACTGATTTTTTCAGCTCACCCATGTTTCTTGCTAACTCAAATACTCTTCCATCTCCGACCACCGGAGCCTTTTCGAGTCAGATTTTCGGCTGGCTGAGCACTCCTAAAGAAAATAACAGCCAGCAAGGAGCGGAGAAGGAGCAGAAAATGTTCACTGATTTCTCATTTCAACCGGCAACATCATCTTCATCGTTCAGCGTTCAGCAATCTTCTTCAAGCATGGTTCCATCGGTGGAGGAATCATTGAAAAGTCAACAGCAGCAATCCTGGGGTTTCAACAACCCAAGGCAAGAAGAAAGCCTAGCAGAGAAAACAGAGGTGAAATCCGAGTTTGCGCCAACTCAGAGCTTCAATGCTGCTCCAAAGTCTGATTACACACATTGCAACACTCATTCAACTCAATATGTGAGGGAACAGAAATCAGATGATGGATACAATTGGAGAAAATATGGACAGAAGCAAGTGAAAGGTAGCGAAAATCCGCGGAGTTATTACAAGTGTACATTTCCCAATTGCCCAACAAAGAAGAAGGTGGAGAGATCATTGGATGGACAGATAACTCAAATTGTGTACAAGGGAAGTCACAACCATACTAAGCCTCAGTCGACAAGACGATCAGCCTCTCAACAACAATCGCTTCAAACAAGTTCGTATGGGATTTCTGATCAGTCCGCCTCGACAATATCCAATCCAAAAATTGAAGCTGTCACGCTGCAGGAGGACTCTAACTCAGCCTCAATGGGAGGAGAGGATGAGTTTGAACAAAATTCGCCGATAAGTAACTCAAATGGAGCTGACGATGACAATGAGCCTGAGGCCAAAAGATGGAAGGGAGAAAATGCAAATGAGACGTATGCAGCTCCTGGGAGTAGAATTGTGAAAGAACCTAGAATTGTAGTGCAGACCACAAGTGAGATTGATATTCTGGATGATGGGTATAGATGGAGGAAATATGGACAGAAAGTAGTGAAGGGAAATCCAAACCCAAGGAGCTACTACAAATGTACTTCTACTGGCTGTCCAGTGAGGAAACATGTGGAGCGAGCATCTCACGATATGAGGGCCGTGATCACAACTTATGAAGGGAAGCACAACCACGATGTTCCTGCAGCACGCGGCAGCGGTGGTTATGGTGCCAGTACTAGACCTGCTTCTGATGCCAACACCAACAGCAATGTATCAGTGGCAGCTGTGAGGCCCTTGGCCTTGCCTAATCGTTCTAACAATTTGAGCTACCTAAGCTCTCTTCAGAAGCAGCCAACATCAGAAAGCCAAGCACCTTATACTCTCAAAATGTTGCAGAACACAGGAAGTTACGGATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
WRKY

Protein Analysis

515

Amino Acids

56.78

Weight (kDa)

8.36

Isoelectric Point (pI)

58.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 215 - 270 5.9e-26 WRKY DNA -binding domain
WRKY PF03106 383 - 440 2.2e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1375
AccB1I GGYRCC 1 cut(s) 1352
AccB7I CCANNNNNTGG 1 cut(s) 1044
AccBSI CCGCTC 1 cut(s) 350
AccI GTMKAC 1 cut(s) 824
AccII CGCG 2 cut(s) 703, 1335
AciI CCGC 7 cut(s) 146, 350, 701, 703, 891, 1335, 1341
AcsI RAATTY 1 cut(s) 985
AcuI CTGAAG 1 cut(s) 1451
AfaI GTAC 4 cut(s) 721, 791, 1225, 1360
AfiI CCNNNNNNNGG 4 cut(s) 81, 109, 281, 1044
AflIII ACRYGT 1 cut(s) 1252
AjnI CCWGG 4 cut(s) 63, 205, 485, 1081
Alw21I GWGCWC 1 cut(s) 320
Alw26I GTCTC 1 cut(s) 1061
AlwNI CAGNNNCTG 1 cut(s) 1082
AoxI GGCC 4 cut(s) 1035, 1281, 1415, 1422
ApeKI GCWGC 9 cut(s) 78, 478, 575, 931, 1076, 1328, 1338, 1406, 1474
ApoI RAATTY 1 cut(s) 985
Asp700I GAANNNNTTC 1 cut(s) 368
AspLEI GCGC 1 cut(s) 556
AspS9I GGNCC 3 cut(s) 67, 1281, 1416
AsuHPI GGTGA 3 cut(s) 11, 228, 550
AvaII GGWCC 1 cut(s) 67
AxyI CCTNAGG 1 cut(s) 1032
BanI GGYRCC 1 cut(s) 1352
Bbv12I GWGCWC 1 cut(s) 320
BbvI GCAGC 9 cut(s) 90, 490, 562, 918, 1088, 1340, 1350, 1418, 1486
BccI CCATC 7 cut(s) 277, 454, 641, 761, 1038, 1145, 1155
BceAI ACGGC 1 cut(s) 1268
BcgI CGANNNNNNTGC 2 cut(s) 1307, 1341
BciT130I CCWGG 4 cut(s) 65, 207, 487, 1083
BciVI GTATCC 2 cut(s) 644, 1532
BclI TGATCA 2 cut(s) 883, 1287
BcoDI GTCTC 1 cut(s) 1061
BfaI CTAG 3 cut(s) 521, 1107, 1362
BfmI CTRYAG 2 cut(s) 932, 1326
BfuAI ACCTGC 1 cut(s) 1375
BfuI GTATCC 2 cut(s) 644, 1532
BlpI GCTNAGC 1 cut(s) 314
BmcAI AGTACT 1 cut(s) 1360
Bme1390I CCNGG 4 cut(s) 65, 207, 487, 1083
Bme18I GGWCC 1 cut(s) 67
BmgT120I GGNCC 3 cut(s) 67, 1281, 1416
BmiI GGNNCC 3 cut(s) 286, 444, 1354
BmrFI CCNGG 4 cut(s) 65, 207, 487, 1083
BmsI GCATC 2 cut(s) 1274, 1366
BpmI CTGGAG 2 cut(s) 103, 125
Bpu10I CCTNAGC 1 cut(s) 1457
Bpu1102I GCTNAGC 1 cut(s) 314
BsaJI CCNNGG 7 cut(s) 205, 486, 503, 701, 1082, 1205, 1419
BsaWI WCCGGW 1 cut(s) 281
Bsc4I CCNNNNNNNGG 4 cut(s) 81, 109, 281, 1044
Bse118I RCCGGY 2 cut(s) 74, 391
Bse1I ACTGG 3 cut(s) 1237, 1241, 1356
Bse21I CCTNAGG 1 cut(s) 1032
Bse3DI GCAATG 2 cut(s) 598, 1399
BseBI CCWGG 4 cut(s) 65, 207, 487, 1083
BseDI CCNNGG 7 cut(s) 205, 486, 503, 701, 1082, 1205, 1419
BseGI GGATG 3 cut(s) 772, 976, 1153
BseLI CCNNNNNNNGG 4 cut(s) 81, 109, 281, 1044
BseMI GCAATG 2 cut(s) 598, 1399
BseMII CTCAG 5 cut(s) 305, 575, 833, 963, 1023
BseNI ACTGG 3 cut(s) 1237, 1241, 1356
BseRI GAGGAG 4 cut(s) 14, 156, 159, 978
BseXI GCAGC 9 cut(s) 90, 490, 562, 918, 1088, 1340, 1350, 1418, 1486
BseYI CCCAGC 1 cut(s) 87
BsgI GTGCAG 1 cut(s) 1139
Bsh1236I CGCG 2 cut(s) 703, 1335
Bsh1285I CGRYCG 1 cut(s) 103
BshFI GGCC 4 cut(s) 1037, 1283, 1417, 1424
BshNI GGYRCC 1 cut(s) 1352
BsiEI CGRYCG 1 cut(s) 103
BsiHKAI GWGCWC 1 cut(s) 320
BsiSI CCGG 3 cut(s) 75, 282, 392
BslFI GGGAC 1 cut(s) 105
BslI CCNNNNNNNGG 4 cut(s) 81, 109, 281, 1044
BsmAI GTCTC 1 cut(s) 1061
BsmBI CGTCTC 1 cut(s) 1061
BsmFI GGGAC 1 cut(s) 105
BsnI GGCC 4 cut(s) 1037, 1283, 1417, 1424
Bsp1286I GDGCHC 1 cut(s) 320
Bsp1407I TGTACA 2 cut(s) 719, 789
Bsp143I GATC 4 cut(s) 758, 833, 883, 1287
Bsp1720I GCTNAGC 1 cut(s) 314
BspACI CCGC 7 cut(s) 146, 350, 701, 703, 891, 1335, 1341
BspANI GGCC 4 cut(s) 1037, 1283, 1417, 1424
BspCNI CTCAG 5 cut(s) 306, 574, 832, 962, 1024
BspFNI CGCG 2 cut(s) 703, 1335
BspLI GGNNCC 3 cut(s) 286, 444, 1354
BspMAI CTGCAG 2 cut(s) 936, 1330
BspMI ACCTGC 1 cut(s) 1375
BspT107I GGYRCC 1 cut(s) 1352
BsrBI CCGCTC 1 cut(s) 350
BsrDI GCAATG 2 cut(s) 598, 1399
BsrFI RCCGGY 2 cut(s) 74, 391
BsrGI TGTACA 2 cut(s) 719, 789
BsrI ACTGG 3 cut(s) 1237, 1241, 1356
BssAI RCCGGY 2 cut(s) 74, 391
BssECI CCNNGG 7 cut(s) 205, 486, 503, 701, 1082, 1205, 1419
BssMI GATC 4 cut(s) 758, 833, 883, 1287
BssT1I CCWWGG 3 cut(s) 503, 1205, 1419
Bst2UI CCWGG 4 cut(s) 65, 207, 487, 1083
Bst4CI ACNGT 1 cut(s) 104
Bst6I CTCTTC 2 cut(s) 270, 1470
BstAUI TGTACA 2 cut(s) 719, 789
BstC8I GCNNGC 4 cut(s) 311, 341, 1263, 1333
BstDEI CTNAG 7 cut(s) 314, 561, 813, 819, 949, 1032, 1457
BstDSI CCRYGG 1 cut(s) 701
BstF5I GGATG 3 cut(s) 772, 976, 1153
BstFNI CGCG 2 cut(s) 703, 1335
BstHHI GCGC 1 cut(s) 556
BstKTI GATC 4 cut(s) 761, 836, 886, 1290
BstMAI GTCTC 1 cut(s) 1061
BstMBI GATC 4 cut(s) 758, 833, 883, 1287
BstMCI CGRYCG 1 cut(s) 103
BstMWI GCNNNNNNNGC 4 cut(s) 84, 315, 1376, 1458
BstNI CCWGG 4 cut(s) 65, 207, 487, 1083
BstNSI RCATGY 1 cut(s) 1256
BstSCI CCNGG 4 cut(s) 63, 205, 485, 1081
BstSFI CTRYAG 2 cut(s) 932, 1326
BstUI CGCG 2 cut(s) 703, 1335
BstV1I GCAGC 9 cut(s) 90, 490, 562, 918, 1088, 1340, 1350, 1418, 1486
Bsu36I CCTNAGG 1 cut(s) 1032
BsuI GTATCC 2 cut(s) 644, 1532
BsuRI GGCC 4 cut(s) 1037, 1283, 1417, 1424
BtgI CCRYGG 1 cut(s) 701
BtsCI GGATG 3 cut(s) 772, 976, 1153
BtsIMutI CAGTG 3 cut(s) 372, 1248, 1407
BveI ACCTGC 1 cut(s) 1375
Cac8I GCNNGC 4 cut(s) 311, 341, 1263, 1333
CaiI CAGNNNCTG 1 cut(s) 1082
CfoI GCGC 1 cut(s) 556
Cfr10I RCCGGY 2 cut(s) 74, 391
Cfr13I GGNCC 3 cut(s) 67, 1281, 1416
Cfr42I CCGCGG 1 cut(s) 704
Csp6I GTAC 4 cut(s) 720, 790, 1224, 1359
CviAII CATG 5 cut(s) 37, 43, 241, 439, 1253
CviQI GTAC 4 cut(s) 720, 790, 1224, 1359
DdeI CTNAG 7 cut(s) 314, 561, 813, 819, 949, 1032, 1457
DpnI GATC 4 cut(s) 760, 835, 885, 1289
DpnII GATC 4 cut(s) 758, 833, 883, 1287
Eam1104I CTCTTC 2 cut(s) 270, 1470
EarI CTCTTC 2 cut(s) 270, 1470
EciI GGCGGA 1 cut(s) 880
Eco130I CCWWGG 3 cut(s) 503, 1205, 1419
Eco47I GGWCC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 1451
Eco81I CCTNAGG 1 cut(s) 1032
EcoO109I RGGNCCY 1 cut(s) 1416
EcoRII CCWGG 4 cut(s) 63, 205, 485, 1081
EcoT14I CCWWGG 3 cut(s) 503, 1205, 1419
ErhI CCWWGG 3 cut(s) 503, 1205, 1419
Esp3I CGTCTC 1 cut(s) 1061
FaeI CATG 5 cut(s) 40, 46, 244, 442, 1256
FaqI GGGAC 1 cut(s) 105
FatI CATG 5 cut(s) 36, 42, 240, 438, 1252
FbaI TGATCA 2 cut(s) 883, 1287
FblI GTMKAC 1 cut(s) 824
FokI GGATG 3 cut(s) 779, 983, 1160
FspBI CTAG 3 cut(s) 521, 1107, 1362
GlaI GCGC 1 cut(s) 555
GsaI CCCAGC 1 cut(s) 91
GsuI CTGGAG 2 cut(s) 103, 125
HaeIII GGCC 4 cut(s) 1037, 1283, 1417, 1424
HapII CCGG 3 cut(s) 75, 282, 392
HhaI GCGC 1 cut(s) 556
Hin1II CATG 5 cut(s) 40, 46, 244, 442, 1256
Hin6I GCGC 1 cut(s) 554
HinP1I GCGC 1 cut(s) 554
HincII GTYRAC 3 cut(s) 199, 473, 825
HindII GTYRAC 3 cut(s) 199, 473, 825
HinfI GANTC 4 cut(s) 93, 295, 458, 941
HpaI GTTAAC 1 cut(s) 199
HpaII CCGG 3 cut(s) 75, 282, 392
HphI GGTGA 3 cut(s) 11, 228, 550
Hpy166II GTNNAC 6 cut(s) 199, 372, 473, 720, 790, 825
Hpy188III TCNNGA 2 cut(s) 1145, 1271
Hpy8I GTNNAC 6 cut(s) 199, 372, 473, 720, 790, 825
HpyAV CCTTC 8 cut(s) 22, 159, 192, 349, 742, 1042, 1183, 1295
HpyCH4III ACNGT 1 cut(s) 104
HpyCH4IV ACGT 1 cut(s) 1070
HpyCH4V TGCA 7 cut(s) 603, 934, 1061, 1076, 1120, 1328, 1519
HpyF10VI GCNNNNNNNGC 4 cut(s) 84, 315, 1376, 1458
HpyF3I CTNAG 7 cut(s) 314, 561, 813, 819, 949, 1032, 1457
HpySE526I ACGT 1 cut(s) 1070
Hsp92II CATG 5 cut(s) 40, 46, 244, 442, 1256
HspAI GCGC 1 cut(s) 554
Ksp22I TGATCA 2 cut(s) 883, 1287
KspAI GTTAAC 1 cut(s) 199
KspI CCGCGG 1 cut(s) 704
Kzo9I GATC 4 cut(s) 758, 833, 883, 1287
LmnI GCTCC 9 cut(s) 84, 284, 347, 358, 583, 1010, 1084, 1209, 1258
Lsp1109I GCAGC 9 cut(s) 90, 490, 562, 918, 1088, 1340, 1350, 1418, 1486
LweI GCATC 2 cut(s) 1274, 1366
MaeI CTAG 3 cut(s) 521, 1107, 1362
MaeII ACGT 1 cut(s) 1070
MaeIII GTNAC 5 cut(s) 135, 800, 925, 998, 1532
MalI GATC 4 cut(s) 760, 835, 885, 1289
MbiI CCGCTC 1 cut(s) 350
MboI GATC 4 cut(s) 758, 833, 883, 1287
MboII GAAGA 8 cut(s) 177, 257, 396, 420, 423, 524, 757, 1457
MfeI CAATTG 2 cut(s) 655, 730
MhlI GDGCHC 1 cut(s) 320
MluCI AATT 9 cut(s) 55, 655, 730, 783, 915, 985, 1092, 1110, 1444
MlyI GAGTC 3 cut(s) 87, 304, 935
MmeI TCCRAC 2 cut(s) 123, 299
MroXI GAANNNNTTC 1 cut(s) 368
MseI TTAA 1 cut(s) 198
MslI CAYNNNNRTG 1 cut(s) 41
MspA1I CMGCKG 4 cut(s) 81, 703, 1341, 1409
MspI CCGG 3 cut(s) 75, 282, 392
MspR9I CCNGG 4 cut(s) 65, 207, 487, 1083
MunI CAATTG 2 cut(s) 655, 730
MvaI CCWGG 4 cut(s) 65, 207, 487, 1083
MvnI CGCG 2 cut(s) 703, 1335
MwoI GCNNNNNNNGC 4 cut(s) 84, 315, 1376, 1458
NdeII GATC 4 cut(s) 758, 833, 883, 1287
NlaIII CATG 5 cut(s) 40, 46, 244, 442, 1256
NlaIV GGNNCC 3 cut(s) 286, 444, 1354
NmuCI GTSAC 3 cut(s) 135, 800, 925
NspI RCATGY 1 cut(s) 1256
PciI ACATGT 1 cut(s) 1252
PdmI GAANNNNTTC 1 cut(s) 368
PfeI GAWTC 1 cut(s) 458
PflMI CCANNNNNTGG 1 cut(s) 1044
PleI GAGTC 3 cut(s) 87, 303, 935
PpsI GAGTC 3 cut(s) 87, 303, 935
PscI ACATGT 1 cut(s) 1252
Psp6I CCWGG 4 cut(s) 63, 205, 485, 1081
PspFI CCCAGC 1 cut(s) 87
PspGI CCWGG 4 cut(s) 63, 205, 485, 1081
PspN4I GGNNCC 3 cut(s) 286, 444, 1354
PspPI GGNCC 3 cut(s) 67, 1281, 1416
PstI CTGCAG 2 cut(s) 936, 1330
PstNI CAGNNNCTG 1 cut(s) 1082
PvuII CAGCTG 2 cut(s) 81, 1409
RsaI GTAC 4 cut(s) 721, 791, 1225, 1360
RsaNI GTAC 4 cut(s) 720, 790, 1224, 1359
RseI CAYNNNNRTG 1 cut(s) 41
SacII CCGCGG 1 cut(s) 704
SalI GTCGAC 1 cut(s) 823
SaqAI TTAA 1 cut(s) 198
Sau3AI GATC 4 cut(s) 758, 833, 883, 1287
Sau96I GGNCC 3 cut(s) 67, 1281, 1416
ScaI AGTACT 1 cut(s) 1360
SchI GAGTC 3 cut(s) 87, 304, 935
ScrFI CCNGG 4 cut(s) 65, 207, 487, 1083
SduI GDGCHC 1 cut(s) 320
SfaNI GCATC 2 cut(s) 1274, 1366
SfcI CTRYAG 2 cut(s) 932, 1326
Sfr303I CCGCGG 1 cut(s) 704
SgrBI CCGCGG 1 cut(s) 704
SinI GGWCC 1 cut(s) 67
SmiMI CAYNNNNRTG 1 cut(s) 41
Sse9I AATT 9 cut(s) 55, 655, 730, 783, 915, 985, 1092, 1110, 1444
SsiI CCGC 7 cut(s) 146, 350, 701, 703, 891, 1335, 1341
SspMI CTAG 3 cut(s) 521, 1107, 1362
StyD4I CCNGG 4 cut(s) 63, 205, 485, 1081
StyI CCWWGG 3 cut(s) 503, 1205, 1419
TaaI ACNGT 1 cut(s) 104
TaiI ACGT 1 cut(s) 1073
TaqI TCGA 3 cut(s) 293, 824, 896
TaqII GACCGA 1 cut(s) 84
TasI AATT 9 cut(s) 55, 655, 730, 783, 915, 985, 1092, 1110, 1444
TatI WGTACW 4 cut(s) 719, 789, 1223, 1358
TauI GCSGC 2 cut(s) 148, 1338
TfiI GAWTC 1 cut(s) 458
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TscAI CASTG 3 cut(s) 379, 1248, 1407
TseFI GTSAC 3 cut(s) 135, 800, 925
TseI GCWGC 9 cut(s) 78, 478, 575, 931, 1076, 1328, 1338, 1406, 1474
Tsp45I GTSAC 3 cut(s) 135, 800, 925
TspDTI ATGAA 4 cut(s) 53, 59, 396, 1314
TspGWI ACGGA 1 cut(s) 125
TspRI CASTG 3 cut(s) 379, 1248, 1407
Van91I CCANNNNNTGG 1 cut(s) 1044
VpaK11BI GGWCC 1 cut(s) 67
XapI RAATTY 1 cut(s) 985
XceI RCATGY 1 cut(s) 1256
XmiI GTMKAC 1 cut(s) 824
XmnI GAANNNNTTC 1 cut(s) 368
XspI CTAG 3 cut(s) 521, 1107, 1362
ZrmI AGTACT 1 cut(s) 1360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.