Prupe.6G286000_v2.0.a1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0001456
Physical Location & Seq
Reverse (-)
26503630 .. 26506039
2410 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G286000.1

Sequence Viewer

Length: 1608 bp
ATGACTTCCTCTTTCACTCACCTCCTCACAAGTAACATGAACAACATGGACAGCAATATTGACCAGGAAAGAACTAATTGGGGACTTTCAGACTATTCTTCAGGCAGTTTAATGGACAGAAACGGCAATGAAATCCCAAACTTCAAGTCACTTCAGCCCCCTTCTCTGCCTATGTCTCCTCCCCCTGTTTCCCCTTCTTCGTACTTGGCTTCCACACCAGCTTTTAGCCCAACTGATTTTTTCAGCTCACCCATGTTCCTATCTTCCTCTAATACTCTTCAATCTCCAACAAGTGGAGCTTTCTCTAGTCAAATCTTTGACTGGATGAGTAATTCTAAAGAAACCCAGCAAGGAATGGAGAGGGAACAGAAAATGTTCTCTGATTTCTCTTTCCAACCAGAAACAAGGCCTGCTGCAACATCATCCTCAACCTTTAACATTCAAGCTTCTTCAAACATGGCTTCAGAAGAATCGTTGAAAACAGGACAGAAACCATGGGATTTCAACAGAATCTCAAGGCAAGCTGATTCTTTAACAGAGAAGACAGGAGTTAAGTCTGAATTTGAGCCACTGCAGAGCATCTTCCCCGAGATTGGCACAAACCAAACAAATATGCAGAGCAATGGACCTAGTGGGGCTCCTAAGCCTGATACAATACATTGCACTCAATCGTCTCAATTTGTCAGAGAGCAAAAATCAGATGATGGGTTCAATTGGAGGAAATATGGACAGAAACAAGTGAAGGGCAGTGAAAATCCGCGGAGTTATTACAAGTGCACGTATCCGAATTGCTCAACAAAGAAAAAGGTTGAGAGATCATTGGATGGACATATCACTCAAATTGTATACAAGGGAAGTCACAACCATCCCAAGCCTCAGTCTACAAGAAGATCAACCTCTCAGTCAATTCAGGGTTCTTCATATGGCATTTCTGATCAATCTGTGCCAACAATATCCAATCCGAAAATCGAATCTGTCACGATGCAGGAGGACTCTTCAGCCTCAATTGGAGAGGATGAGTTTGAACAAAACTCACCAATAAGTAATTCAGGAGGAGCTGAAGATGAAAATGAGCCTGAGGCCAAAAGATGGAAGGGAGAAAATGCAAACGATCAGCCCTTTTCGGCTCCTGGGAGTAGAATTGTAAAAGAGCCAAGAATTGTAGTGCAGACAACAAGTGAAATAGATATTCTGGATGATGGGTATAGATGGAGGAAATATGGACAGAAAGTAGTGAAGGGAAATCCAAATCCAAGGAGCTACTACAAATGCACATCCGTAGGTTGTCCGGTGAGGAAACATGTGGAGCGAGCTTCACATGATACAAGGGCTGTGATCACCACATATGAAGGGAAACACAACCATGATGTTCCTGCAGCGCGCGGCAGCGGCAGTTATAGCAATGCAAATAGACCTGCTTCTGATAATGGCAGCAACAACAACAGCAATGTGTCCATGGCTGTGAGGCCCTTAGCCTTGCCTAATCATTCGAACTTGAGCTACCTCAACTCTCTTCAGAATACAAGGCAGCCAACAACAGAAAGGCAATCACCCTATACCCTCAAAATGTTACAGAGTGAAGGAAGTTATGGATTTCCAGAGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
WRKY

Protein Analysis

536

Amino Acids

59.34

Weight (kDa)

6.81

Isoelectric Point (pI)

62.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1423
AccB7I CCANNNNNTGG 2 cut(s) 293, 1089
AccI GTMKAC 2 cut(s) 846, 881
AccII CGCG 3 cut(s) 760, 1381, 1383
AciI CCGC 4 cut(s) 758, 760, 1383, 1389
AcsI RAATTY 1 cut(s) 560
AcuI CTGAAG 6 cut(s) 84, 137, 447, 981, 1080, 1499
AfaI GTAC 1 cut(s) 203
AfiI CCNNNNNNNGG 3 cut(s) 293, 593, 1089
AflIII ACRYGT 1 cut(s) 1300
AgsI TTSAA 8 cut(s) 145, 281, 443, 453, 478, 505, 712, 1025
AjnI CCWGG 2 cut(s) 63, 1129
AjuI GAANNNNNNNTTGG 2 cut(s) 1030, 1062
AluBI AGCT 9 cut(s) 221, 246, 299, 446, 524, 1058, 1260, 1313, 1500
AluI AGCT 9 cut(s) 221, 246, 299, 446, 524, 1058, 1260, 1313, 1500
Alw21I GWGCWC 1 cut(s) 779
Alw26I GTCTC 2 cut(s) 180, 678
Alw44I GTGCAC 1 cut(s) 775
Ama87I CYCGRG 1 cut(s) 587
AoxI GGCC 3 cut(s) 407, 1080, 1466
ApaLI GTGCAC 1 cut(s) 775
ApeKI GCWGC 5 cut(s) 413, 1376, 1386, 1431, 1528
ApoI RAATTY 1 cut(s) 560
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
Asp700I GAANNNNTTC 1 cut(s) 374
AspLEI GCGC 2 cut(s) 1381, 1383
AspS9I GGNCC 2 cut(s) 626, 1467
AsuHPI GGTGA 6 cut(s) 11, 240, 1026, 1303, 1330, 1542
AsuII TTCGAA 1 cut(s) 1490
AvaI CYCGRG 1 cut(s) 587
AvaII GGWCC 1 cut(s) 626
AxyI CCTNAGG 1 cut(s) 1077
BaeGI GKGCMC 1 cut(s) 779
BanII GRGCYC 1 cut(s) 640
BbsI GAAGAC 1 cut(s) 548
Bbv12I GWGCWC 1 cut(s) 779
BbvI GCAGC 5 cut(s) 400, 1388, 1398, 1443, 1540
BccI CCATC 6 cut(s) 698, 818, 873, 1083, 1193, 1203
BceAI ACGGC 1 cut(s) 139
BciT130I CCWGG 2 cut(s) 65, 1131
BciVI GTATCC 1 cut(s) 792
BclI TGATCA 2 cut(s) 934, 1335
BcoDI GTCTC 2 cut(s) 180, 678
BfaI CTAG 2 cut(s) 306, 630
BfmI CTRYAG 2 cut(s) 572, 1374
BfuAI ACCTGC 1 cut(s) 1423
BfuI GTATCC 1 cut(s) 792
BisI GCNGC 7 cut(s) 414, 1377, 1384, 1387, 1390, 1432, 1529
BlsI GCNGC 7 cut(s) 415, 1378, 1385, 1388, 1391, 1433, 1530
Bme1390I CCNGG 2 cut(s) 65, 1131
Bme18I GGWCC 1 cut(s) 626
BmeT110I CYCGRG 1 cut(s) 587
BmgT120I GGNCC 2 cut(s) 626, 1467
BmiI GGNNCC 2 cut(s) 639, 1128
BmrFI CCNGG 2 cut(s) 65, 1131
BmsI GCATC 2 cut(s) 588, 972
BpiI GAAGAC 1 cut(s) 548
Bpu10I CCTNAGC 2 cut(s) 642, 1471
Bpu14I TTCGAA 1 cut(s) 1490
BpuEI CTTGAG 2 cut(s) 499, 1516
BsaAI YACGTR 1 cut(s) 780
BsaJI CCNNGG 5 cut(s) 494, 758, 1130, 1253, 1455
BsaWI WCCGGW 1 cut(s) 1288
Bsc4I CCNNNNNNNGG 3 cut(s) 293, 593, 1089
Bse1I ACTGG 1 cut(s) 326
Bse21I CCTNAGG 1 cut(s) 1077
Bse3DI GCAATG 5 cut(s) 133, 628, 658, 1408, 1453
BseBI CCWGG 2 cut(s) 65, 1131
BseDI CCNNGG 5 cut(s) 494, 758, 1130, 1253, 1455
BseGI GGATG 7 cut(s) 330, 422, 829, 865, 1021, 1201, 1274
BseLI CCNNNNNNNGG 3 cut(s) 293, 593, 1089
BseMI GCAATG 5 cut(s) 133, 628, 658, 1408, 1453
BseMII CTCAG 3 cut(s) 890, 914, 1068
BseNI ACTGG 1 cut(s) 326
BsePI GCGCGC 1 cut(s) 1379
BseRI GAGGAG 3 cut(s) 14, 168, 1068
BseSI GKGCMC 1 cut(s) 779
BseXI GCAGC 5 cut(s) 400, 1388, 1398, 1443, 1540
BseYI CCCAGC 1 cut(s) 345
BsgI GTGCAG 1 cut(s) 1187
Bsh1236I CGCG 3 cut(s) 760, 1381, 1383
BshFI GGCC 3 cut(s) 409, 1082, 1468
BsiHKAI GWGCWC 1 cut(s) 779
BsiHKCI CYCGRG 1 cut(s) 587
BsiSI CCGG 1 cut(s) 1289
BslFI GGGAC 1 cut(s) 96
BslI CCNNNNNNNGG 3 cut(s) 293, 593, 1089
BsmAI GTCTC 2 cut(s) 180, 678
BsmBI CGTCTC 1 cut(s) 678
BsmFI GGGAC 1 cut(s) 96
BsnI GGCC 3 cut(s) 409, 1082, 1468
BsoBI CYCGRG 1 cut(s) 587
Bsp119I TTCGAA 1 cut(s) 1490
Bsp1286I GDGCHC 2 cut(s) 640, 779
Bsp143I GATC 5 cut(s) 815, 890, 934, 1111, 1335
Bsp19I CCATGG 2 cut(s) 494, 1455
BspACI CCGC 4 cut(s) 758, 760, 1383, 1389
BspANI GGCC 3 cut(s) 409, 1082, 1468
BspCNI CTCAG 3 cut(s) 889, 913, 1069
BspFNI CGCG 3 cut(s) 760, 1381, 1383
BspLI GGNNCC 2 cut(s) 639, 1128
BspMAI CTGCAG 2 cut(s) 576, 1378
BspMI ACCTGC 1 cut(s) 1423
BspT104I TTCGAA 1 cut(s) 1490
BsrDI GCAATG 5 cut(s) 133, 628, 658, 1408, 1453
BsrI ACTGG 1 cut(s) 326
BssECI CCNNGG 5 cut(s) 494, 758, 1130, 1253, 1455
BssHII GCGCGC 1 cut(s) 1379
BssMI GATC 5 cut(s) 815, 890, 934, 1111, 1335
BssNAI GTATAC 1 cut(s) 847
BssT1I CCWWGG 3 cut(s) 494, 1253, 1455
Bst1107I GTATAC 1 cut(s) 847
Bst2UI CCWGG 2 cut(s) 65, 1131
Bst6I CTCTTC 3 cut(s) 282, 1000, 1518
BstBAI YACGTR 1 cut(s) 780
BstBI TTCGAA 1 cut(s) 1490
BstC8I GCNNGC 4 cut(s) 411, 522, 1311, 1381
BstDEI CTNAG 5 cut(s) 642, 876, 900, 1077, 1471
BstDSI CCRYGG 3 cut(s) 494, 758, 1455
BstF5I GGATG 7 cut(s) 330, 422, 829, 865, 1021, 1201, 1274
BstFNI CGCG 3 cut(s) 760, 1381, 1383
BstHHI GCGC 2 cut(s) 1381, 1383
BstKTI GATC 5 cut(s) 818, 893, 937, 1114, 1338
BstMAI GTCTC 2 cut(s) 180, 678
BstMBI GATC 5 cut(s) 815, 890, 934, 1111, 1335
BstMWI GCNNNNNNNGC 2 cut(s) 1389, 1398
BstNI CCWGG 2 cut(s) 65, 1131
BstNSI RCATGY 1 cut(s) 1304
BstSCI CCNGG 2 cut(s) 63, 1129
BstSFI CTRYAG 2 cut(s) 572, 1374
BstSLI GKGCMC 1 cut(s) 779
BstUI CGCG 3 cut(s) 760, 1381, 1383
BstV1I GCAGC 5 cut(s) 400, 1388, 1398, 1443, 1540
BstV2I GAAGAC 1 cut(s) 548
BstZ17I GTATAC 1 cut(s) 847
Bsu36I CCTNAGG 1 cut(s) 1077
BsuI GTATCC 1 cut(s) 792
BsuRI GGCC 3 cut(s) 409, 1082, 1468
BtgI CCRYGG 3 cut(s) 494, 758, 1455
BtsCI GGATG 7 cut(s) 330, 422, 829, 865, 1021, 1201, 1274
BtsI GCAGTG 2 cut(s) 569, 754
BtsIMutI CAGTG 2 cut(s) 569, 754
BveI ACCTGC 1 cut(s) 1423
Cac8I GCNNGC 4 cut(s) 411, 522, 1311, 1381
CfoI GCGC 2 cut(s) 1381, 1383
Cfr13I GGNCC 2 cut(s) 626, 1467
Cfr42I CCGCGG 1 cut(s) 761
Csp6I GTAC 1 cut(s) 202
CviAII CATG 9 cut(s) 37, 46, 253, 457, 495, 1301, 1319, 1364, 1456
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 5 cut(s) 642, 876, 900, 1077, 1471
DpnI GATC 5 cut(s) 817, 892, 936, 1113, 1337
DpnII GATC 5 cut(s) 815, 890, 934, 1111, 1335
Eam1104I CTCTTC 3 cut(s) 282, 1000, 1518
EarI CTCTTC 3 cut(s) 282, 1000, 1518
Eco130I CCWWGG 3 cut(s) 494, 1253, 1455
Eco147I AGGCCT 1 cut(s) 409
Eco24I GRGCYC 1 cut(s) 640
Eco47I GGWCC 1 cut(s) 626
Eco57I CTGAAG 6 cut(s) 84, 137, 447, 981, 1080, 1499
Eco81I CCTNAGG 1 cut(s) 1077
Eco88I CYCGRG 1 cut(s) 587
EcoO109I RGGNCCY 1 cut(s) 1467
EcoRII CCWGG 2 cut(s) 63, 1129
EcoT14I CCWWGG 3 cut(s) 494, 1253, 1455
EcoT38I GRGCYC 1 cut(s) 640
ErhI CCWWGG 3 cut(s) 494, 1253, 1455
Esp3I CGTCTC 1 cut(s) 678
FaeI CATG 9 cut(s) 40, 49, 256, 460, 498, 1304, 1322, 1367, 1459
FalI AAGNNNNNCTT 2 cut(s) 283, 315
FaqI GGGAC 1 cut(s) 96
FatI CATG 9 cut(s) 36, 45, 252, 456, 494, 1300, 1318, 1363, 1455
FauNDI CATATG 2 cut(s) 922, 1345
FbaI TGATCA 2 cut(s) 934, 1335
FblI GTMKAC 2 cut(s) 846, 881
Fnu4HI GCNGC 7 cut(s) 414, 1377, 1384, 1387, 1390, 1432, 1529
FokI GGATG 7 cut(s) 337, 409, 836, 852, 1028, 1208, 1261
FriOI GRGCYC 1 cut(s) 640
Fsp4HI GCNGC 7 cut(s) 414, 1377, 1384, 1387, 1390, 1432, 1529
FspBI CTAG 2 cut(s) 306, 630
GlaI GCGC 2 cut(s) 1380, 1382
GluI GCNGC 7 cut(s) 414, 1377, 1384, 1387, 1390, 1432, 1529
GsaI CCCAGC 1 cut(s) 349
HaeIII GGCC 3 cut(s) 409, 1082, 1468
HapII CCGG 1 cut(s) 1289
HhaI GCGC 2 cut(s) 1381, 1383
Hin1II CATG 9 cut(s) 40, 49, 256, 460, 498, 1304, 1322, 1367, 1459
Hin6I GCGC 2 cut(s) 1379, 1381
HinP1I GCGC 2 cut(s) 1379, 1381
HindIII AAGCTT 1 cut(s) 444
HinfI GANTC 5 cut(s) 470, 510, 527, 971, 992
HpaII CCGG 1 cut(s) 1289
HphI GGTGA 6 cut(s) 11, 240, 1026, 1303, 1330, 1542
Hpy166II GTNNAC 3 cut(s) 777, 847, 882
Hpy188III TCNNGA 4 cut(s) 979, 1050, 1193, 1598
Hpy8I GTNNAC 3 cut(s) 777, 847, 882
HpyAV CCTTC 7 cut(s) 171, 204, 736, 1087, 1231, 1343, 1574
HpyCH4IV ACGT 1 cut(s) 779
HpyF10VI GCNNNNNNNGC 2 cut(s) 1389, 1398
HpyF3I CTNAG 5 cut(s) 642, 876, 900, 1077, 1471
HpySE526I ACGT 1 cut(s) 779
Hsp92II CATG 9 cut(s) 40, 49, 256, 460, 498, 1304, 1322, 1367, 1459
HspAI GCGC 2 cut(s) 1379, 1381
Ksp22I TGATCA 2 cut(s) 934, 1335
KspI CCGCGG 1 cut(s) 761
Kzo9I GATC 5 cut(s) 815, 890, 934, 1111, 1335
LmnI GCTCC 6 cut(s) 296, 643, 1055, 1132, 1257, 1306
Lsp1109I GCAGC 5 cut(s) 400, 1388, 1398, 1443, 1540
LweI GCATC 2 cut(s) 588, 972
MaeI CTAG 2 cut(s) 306, 630
MaeII ACGT 1 cut(s) 779
MaeIII GTNAC 5 cut(s) 32, 147, 857, 976, 1569
MalI GATC 5 cut(s) 817, 892, 936, 1113, 1337
MboI GATC 5 cut(s) 815, 890, 934, 1111, 1335
MfeI CAATTG 2 cut(s) 712, 1005
MhlI GDGCHC 2 cut(s) 640, 779
MlyI GAGTC 1 cut(s) 986
MmeI TCCRAC 2 cut(s) 311, 418
MroXI GAANNNNTTC 1 cut(s) 374
MseI TTAA 4 cut(s) 110, 435, 533, 552
MslI CAYNNNNRTG 2 cut(s) 1362, 1460
MspA1I CMGCKG 2 cut(s) 760, 1389
MspI CCGG 1 cut(s) 1289
MspR9I CCNGG 2 cut(s) 65, 1131
MunI CAATTG 2 cut(s) 712, 1005
MvaI CCWGG 2 cut(s) 65, 1131
MvnI CGCG 3 cut(s) 760, 1381, 1383
MwoI GCNNNNNNNGC 2 cut(s) 1389, 1398
NcoI CCATGG 2 cut(s) 494, 1455
NdeI CATATG 2 cut(s) 922, 1345
NdeII GATC 5 cut(s) 815, 890, 934, 1111, 1335
NlaIII CATG 9 cut(s) 40, 49, 256, 460, 498, 1304, 1322, 1367, 1459
NlaIV GGNNCC 2 cut(s) 639, 1128
NmuCI GTSAC 3 cut(s) 147, 857, 976
NspI RCATGY 1 cut(s) 1304
NspV TTCGAA 1 cut(s) 1490
PauI GCGCGC 1 cut(s) 1379
PceI AGGCCT 1 cut(s) 409
PciI ACATGT 1 cut(s) 1300
PdmI GAANNNNTTC 1 cut(s) 374
PfeI GAWTC 4 cut(s) 470, 510, 527, 971
PflMI CCANNNNNTGG 2 cut(s) 293, 1089
PkrI GCNGC 7 cut(s) 415, 1378, 1385, 1388, 1391, 1433, 1530
PleI GAGTC 1 cut(s) 986
PpsI GAGTC 1 cut(s) 986
Ppu21I YACGTR 1 cut(s) 780
PscI ACATGT 1 cut(s) 1300
Psp6I CCWGG 2 cut(s) 63, 1129
PspFI CCCAGC 1 cut(s) 345
PspGI CCWGG 2 cut(s) 63, 1129
PspN4I GGNNCC 2 cut(s) 639, 1128
PspPI GGNCC 2 cut(s) 626, 1467
PsrI GAACNNNNNNTAC 2 cut(s) 1484, 1516
PstI CTGCAG 2 cut(s) 576, 1378
PteI GCGCGC 1 cut(s) 1379
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
RseI CAYNNNNRTG 2 cut(s) 1362, 1460
SacII CCGCGG 1 cut(s) 761
SaqAI TTAA 4 cut(s) 110, 435, 533, 552
SatI GCNGC 7 cut(s) 414, 1377, 1384, 1387, 1390, 1432, 1529
Sau3AI GATC 5 cut(s) 815, 890, 934, 1111, 1335
Sau96I GGNCC 2 cut(s) 626, 1467
SchI GAGTC 1 cut(s) 986
ScrFI CCNGG 2 cut(s) 65, 1131
SduI GDGCHC 2 cut(s) 640, 779
SfaNI GCATC 2 cut(s) 588, 972
SfcI CTRYAG 2 cut(s) 572, 1374
Sfr303I CCGCGG 1 cut(s) 761
SfuI TTCGAA 1 cut(s) 1490
SgrBI CCGCGG 1 cut(s) 761
SinI GGWCC 1 cut(s) 626
SmiMI CAYNNNNRTG 2 cut(s) 1362, 1460
SmlI CTYRAG 2 cut(s) 514, 1495
SmoI CTYRAG 2 cut(s) 514, 1495
SseBI AGGCCT 1 cut(s) 409
SsiI CCGC 4 cut(s) 758, 760, 1383, 1389
SspI AATATT 1 cut(s) 58
SspMI CTAG 2 cut(s) 306, 630
StuI AGGCCT 1 cut(s) 409
StyD4I CCNGG 2 cut(s) 63, 1129
StyI CCWWGG 3 cut(s) 494, 1253, 1455
TaiI ACGT 1 cut(s) 782
TaqI TCGA 2 cut(s) 969, 1490
TauI GCSGC 2 cut(s) 1386, 1392
TfiI GAWTC 4 cut(s) 470, 510, 527, 971
Tru1I TTAA 4 cut(s) 110, 435, 533, 552
Tru9I TTAA 4 cut(s) 110, 435, 533, 552
TscAI CASTG 2 cut(s) 576, 754
TseFI GTSAC 3 cut(s) 147, 857, 976
TseI GCWGC 5 cut(s) 413, 1376, 1386, 1431, 1528
Tsp45I GTSAC 3 cut(s) 147, 857, 976
TspDTI ATGAA 5 cut(s) 53, 144, 909, 1080, 1362
TspGWI ACGGA 1 cut(s) 1267
TspRI CASTG 2 cut(s) 576, 754
Van91I CCANNNNNTGG 2 cut(s) 293, 1089
VneI GTGCAC 1 cut(s) 775
VpaK11BI GGWCC 1 cut(s) 626
XapI RAATTY 1 cut(s) 560
XceI RCATGY 1 cut(s) 1304
XmiI GTMKAC 2 cut(s) 846, 881
XmnI GAANNNNTTC 1 cut(s) 374
XspI CTAG 2 cut(s) 306, 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.