Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0001456

Physical Location & Seq

Genomic Coordinates Reverse (-)

78116263 .. 78119061

Gene Structure 2799 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG490700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1878 bp

ATGGCCTCCTCTTCCGGGAGCTTAGACACCTCCGCAAATTCTCACCCGAGCAACTTCACCTTCTCCACCCACCCATTCATGACCACCTCCTTCTCCGACCTCTTATCCTCGGGCGCCGACACCACAAACCCAATAGCCACCGCAACCCGAAACTCCGGGTACGGGTTATCGGATCGTATTGCGGAGCGAACGGGGTCGGGTGTCCCCAAGTTCAAGTCCCTCCCGCCTCCCCAGCTCCCCATTTCGCCTCCCTCTGTTGTGTCTCCCTCTTCCTACTTTGCAATCCCACCTGGGATGAGCCCCGCCGAGCTTCTCGACTCTCCTGTGCTTCTCAGCTCTTCCAACATTCTTCCATCTCCAACTACTGGGAGTTTTGCAGCTCAAGCCATTAATTGGAGGACCAATTCTGGTTCTGGTGCTAACAACAATAACAACAACCGGAGTGTTAAAGAGGAGCACAAGAACTACTCAGATTTCTCTTTCCAAACCCAAACCAGGGCCCCTCCAACTTCATCATCAGCAATTTTCCAATCCTCAAACAGCTCAATTCAGACTGCACAAGAGCAGGCATGGAATAACTATCTAGGACCTCCCAAACAAGATAACTTTTCTTCAGGGAAGAGTATGGTGAAACCTGAGTATGGTTCTTCAGTGCAGAGCTTCTCTTCTGAAATGGGGACTAATATGCAGAGCAATAACAATGCTCAGCCCAACAGCAGTGGTGGTTTCCAATCAGATCAGTACAGCAACTACAATAACAATAACAACAACAACAACAACAACAACAGCAACAACAACCAGCTGCAATCCCATCAGTGCTTGAACAGGAGATCAGATGACGGGTTCAATTGGAGGAAATATGGCCAGAAACAAGTGAAGGGAAGCGAAAATCCGCGGAGCTATTACAAGTGTACTTATCCGAATTGCCCAACTAAGAAAAAGGTCGAGAGGTCGTTGGATGGACAAATCACCGAGATAGTTTACAAGGGTAATCATAACCATCCCAAGCCACAGAATACTAGAAGGTCGGGTTCATCAGCGGCTTCTTCCCATGCAATCCACGCGTCCAATCACACCACCAATGACATCCCGGATCAGTCTTTGGGCTCTCATGGTAATTCACAAATGGATAACTCCATTGGAACACCGGAGAATTCATCCAATTTTTCAAATGGGGATGATGAGTTTGATCAGAGCTCTCAGAGAAGCAAGTCAGGAGGAGGGGATGAGTATGATGAGGATGAGCCTAATGCCAAAAGATGGAAAAAAGAAGGTGACAATGAATGTATTTCAGCACCTGGGAGTAGAACAGTGAGAGAACCAAGAGTTGTAGTTCAAACAACAAGTGACATTGATATTCTTGATGATGGGTACAGGTGGAGGAAGTATGGTCAGAAAGTAGTAAAGGGCAACCCAAATCCAAGGAGCTACTACAAGTGCACAAATCCAGGCTGTCCAGTGAGGAAGCACGTAGAGCGAGCTTCACATGATCTTAGAGCAGTGATCACAACCTACGAAGGGAAGCACAACCATGATGTTCCTGCAGCCCGTGGCAGTGGTTCTGCCAACAGGCCTTTACCAAGCCACAATACCAACAACAACCACAATGTACCCACAGCAATGAGGCCTATGACCCATCACAGTACTAACAATGCTGCTAACAACAACCCTCTTGACAATCTAAGGCTACCGACATCAGAAGGGCAAGCACCCTTTACTCTCGAGATGTTGCAGAGCCCAGCAAGTTATGGATTTGCAGGGTTTGATAACCCCATGGGGTCATACATGAACCAAACACAGCTCAATGACAACAACATTTTTTCTAAAACCAAGGACGAGCCAAGAGATGACGCATTTTTTGAATCATTGCTGTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006725 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009409 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009700 GO:0009889 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010035 GO:0010120 GO:0010200 GO:0010243 GO:0010286 GO:0010468 GO:0010506 GO:0010508 GO:0010556 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019748 GO:0031323 GO:0031325 GO:0031326 GO:0031329 GO:0031331 GO:0033554 GO:0034605 GO:0034641 GO:0042221 GO:0042430 GO:0042435 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044424 GO:0044464 GO:0044550 GO:0046217 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0065007 GO:0070370 GO:0071704 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

WRKY

Protein Analysis

625

Amino Acids

68.35

Weight (kDa)

7.29

Isoelectric Point (pI)

52.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	279 - 335	1.6e-26	WRKY DNA -binding domain
WRKY	PF03106	455 - 512	2.3e-26	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001456)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07100 AT5G07100 AT5G07100 AT5G07100 AT5G07100
fragaria_vesca	FvH4_3g39850 FvH4_3g39850 FvH4_3g39850 FvH4_3g39850 FvH4_3g39850 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510
malus_domestica	MD03G1057400.v1.1 MD04G1167700.v1.1 MD06G1115200.v1.1 MD11G1059400.v1.1 MD12G1181000.v1.1
prunus_persica	Prupe.6G046900_v2.0.a1 Prupe.6G286000_v2.0.a1
pyrus_communis	pycom03g04580 pycom04g14890 pycom06g10790 pycom11g04930 pycom12g17060
rosa_chinensis	RchiOBHm_Chr3g0461481 RchiOBHm_Chr5g0071811
rosa_laevigata	RLG00000024931 RLG00000036250
rosa_multiflora	Rmu_sc0002677.1_g000005 Rmu_ssc0000127.1_g000054
rosa_roxburghii	Rroxscaffold_1G00009350 Rroxscaffold_6G00418250 Rroxscaffold_6G00418580
rosa_rugosa	Rorug03G0051000 Rorug03G0051100.1 Rorug05G0414700
rosa_samantha	Rh3AG108800 Rh3BG112700 Rh3CG114200 Rh3DG114300 Rh5AG471000 Rh5BG490700 Rh5CG514700 Rh5DG502000
rosa_wichuraiana	Rw3G009250 Rw5G043770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	113
AccB7I	CCANNNNNTGG	3 cut(s)	365, 393, 1260
AccII	CGCG	2 cut(s)	895, 1064
AciI	CCGC	8 cut(s)	33, 141, 182, 224, 303, 893, 895, 1040
AclWI	GGATC	2 cut(s)	180, 1101
AcoI	YGGCCR	1 cut(s)	862
AcsI	RAATTY	2 cut(s)	37, 1153
AcuI	CTGAAG	2 cut(s)	597, 633
AcyI	GRCGYC	1 cut(s)	114
AfaI	GTAC	6 cut(s)	161, 743, 913, 1373, 1611, 1645
AfiI	CCNNNNNNNGG	9 cut(s)	15, 162, 365, 393, 495, 496, 641, 1260, 1518
AflIII	ACRYGT	1 cut(s)	1062
AgsI	TTSAA	6 cut(s)	214, 823, 847, 1170, 1337, 1862
AjnI	CCWGG	4 cut(s)	289, 494, 1297, 1447
Alw21I	GWGCWC	3 cut(s)	459, 1199, 1442
Alw26I	GTCTC	1 cut(s)	267
Alw44I	GTGCAC	1 cut(s)	1438
AlwI	GGATC	2 cut(s)	180, 1101
AlwNI	CAGNNNCTG	1 cut(s)	1298
Ama87I	CYCGRG	3 cut(s)	46, 109, 1721
AoxI	GGCC	5 cut(s)	3, 498, 862, 1571, 1625
ApaI	GGGCCC	1 cut(s)	502
ApaLI	GTGCAC	1 cut(s)	1438
ApeKI	GCWGC	4 cut(s)	377, 802, 1544, 1655
ApoI	RAATTY	2 cut(s)	37, 1153
ArsI	GACNNNNNNTTYG	2 cut(s)	1841, 1873
AseI	ATTAAT	1 cut(s)	390
AspLEI	GCGC	1 cut(s)	116
AspS9I	GGNCC	4 cut(s)	399, 498, 499, 587
AsuC2I	CCSGG	3 cut(s)	16, 157, 1091
AsuHPI	GGTGA	5 cut(s)	35, 49, 640, 961, 1286
AvaI	CYCGRG	3 cut(s)	46, 109, 1721
AvaII	GGWCC	2 cut(s)	399, 587
BaeGI	GKGCMC	2 cut(s)	502, 1442
BalI	TGGCCA	1 cut(s)	864
BanI	GGYRCC	1 cut(s)	113
BanII	GRGCYC	5 cut(s)	302, 502, 1109, 1199, 1739
Bbv12I	GWGCWC	3 cut(s)	459, 1199, 1442
BbvI	GCAGC	4 cut(s)	389, 789, 1556, 1642
BccI	CCATC	7 cut(s)	361, 819, 953, 1008, 1254, 1361, 1644
BciT130I	CCWGG	4 cut(s)	291, 496, 1299, 1449
BclI	TGATCA	2 cut(s)	1189, 1503
BcnI	CCSGG	3 cut(s)	16, 157, 1091
BcoDI	GTCTC	1 cut(s)	267
BfaI	CTAG	2 cut(s)	584, 1020
BfmI	CTRYAG	1 cut(s)	1542
BfoI	RGCGCY	1 cut(s)	117
BisI	GCNGC	5 cut(s)	378, 803, 1041, 1545, 1656
BlpI	GCTNAGC	1 cut(s)	705
BlsI	GCNGC	5 cut(s)	379, 804, 1042, 1546, 1657
BmcAI	AGTACT	1 cut(s)	1645
Bme1390I	CCNGG	7 cut(s)	16, 157, 291, 496, 1091, 1299, 1449
Bme18I	GGWCC	2 cut(s)	399, 587
BmeT110I	CYCGRG	3 cut(s)	46, 109, 1721
BmgT120I	GGNCC	4 cut(s)	399, 498, 499, 587
BmiI	GGNNCC	3 cut(s)	115, 500, 501
BmrFI	CCNGG	7 cut(s)	16, 157, 291, 496, 1091, 1299, 1449
BmrI	ACTGGG	1 cut(s)	375
BmuI	ACTGGG	1 cut(s)	375
Bpu1102I	GCTNAGC	1 cut(s)	705
BpuEI	CTTGAG	1 cut(s)	366
BpuMI	CCSGG	3 cut(s)	16, 157, 1091
BsaAI	YACGTR	1 cut(s)	1471
BsaHI	GRCGYC	1 cut(s)	114
BsaJI	CCNNGG	9 cut(s)	108, 290, 495, 893, 1298, 1421, 1549, 1773, 1830
BsaWI	WCCGGW	2 cut(s)	438, 1147
Bsc4I	CCNNNNNNNGG	9 cut(s)	15, 162, 365, 393, 495, 496, 641, 1260, 1518
Bse1I	ACTGG	2 cut(s)	370, 1457
Bse3DI	GCAATG	2 cut(s)	1626, 1865
BseBI	CCWGG	4 cut(s)	291, 496, 1299, 1449
BseDI	CCNNGG	9 cut(s)	108, 290, 495, 893, 1298, 1421, 1549, 1773, 1830
BseGI	GGATG	8 cut(s)	300, 964, 1000, 1086, 1157, 1183, 1231, 1246
BseLI	CCNNNNNNNGG	9 cut(s)	15, 162, 365, 393, 495, 496, 641, 1260, 1518
BseMI	GCAATG	2 cut(s)	1626, 1865
BseMII	CTCAG	5 cut(s)	346, 483, 627, 719, 1214
BseNI	ACTGG	2 cut(s)	370, 1457
BseRI	GAGGAG	2 cut(s)	467, 1233
BseSI	GKGCMC	2 cut(s)	502, 1442
BseXI	GCAGC	4 cut(s)	389, 789, 1556, 1642
BseYI	CCCAGC	2 cut(s)	231, 1738
BsgI	GTGCAG	2 cut(s)	540, 674
Bsh1236I	CGCG	2 cut(s)	895, 1064
BshFI	GGCC	5 cut(s)	5, 500, 864, 1573, 1627
BshNI	GGYRCC	1 cut(s)	113
BsiHKAI	GWGCWC	3 cut(s)	459, 1199, 1442
BsiHKCI	CYCGRG	3 cut(s)	46, 109, 1721
BsiSI	CCGG	5 cut(s)	15, 156, 439, 1091, 1148
BslFI	GGGAC	3 cut(s)	188, 202, 691
BslI	CCNNNNNNNGG	9 cut(s)	15, 162, 365, 393, 495, 496, 641, 1260, 1518
BsmAI	GTCTC	1 cut(s)	267
BsmFI	GGGAC	3 cut(s)	188, 202, 691
BsnI	GGCC	5 cut(s)	5, 500, 864, 1573, 1627
BsoBI	CYCGRG	3 cut(s)	46, 109, 1721
Bsp120I	GGGCCC	1 cut(s)	498
Bsp1286I	GDGCHC	7 cut(s)	302, 459, 502, 1109, 1199, 1442, 1739
Bsp143I	GATC	7 cut(s)	172, 736, 830, 1093, 1189, 1489, 1503
Bsp1720I	GCTNAGC	1 cut(s)	705
Bsp19I	CCATGG	1 cut(s)	1773
BspACI	CCGC	8 cut(s)	33, 141, 182, 224, 303, 893, 895, 1040
BspANI	GGCC	5 cut(s)	5, 500, 864, 1573, 1627
BspCNI	CTCAG	5 cut(s)	345, 482, 628, 718, 1213
BspFNI	CGCG	2 cut(s)	895, 1064
BspHI	TCATGA	1 cut(s)	78
BspLI	GGNNCC	3 cut(s)	115, 500, 501
BspMAI	CTGCAG	1 cut(s)	1546
BspPI	GGATC	2 cut(s)	180, 1101
BspQI	GCTCTTC	1 cut(s)	343
BspT107I	GGYRCC	1 cut(s)	113
BsrDI	GCAATG	2 cut(s)	1626, 1865
BsrI	ACTGG	2 cut(s)	370, 1457
BssECI	CCNNGG	9 cut(s)	108, 290, 495, 893, 1298, 1421, 1549, 1773, 1830
BssMI	GATC	7 cut(s)	172, 736, 830, 1093, 1189, 1489, 1503
BssNI	GRCGYC	1 cut(s)	114
BssT1I	CCWWGG	3 cut(s)	1421, 1773, 1830
Bst2UI	CCWGG	4 cut(s)	291, 496, 1299, 1449
Bst4CI	ACNGT	2 cut(s)	1312, 1643
Bst6I	CTCTTC	5 cut(s)	16, 274, 343, 614, 670
BstACI	GRCGYC	1 cut(s)	114
BstBAI	YACGTR	1 cut(s)	1471
BstC8I	GCNNGC	3 cut(s)	567, 1479, 1707
BstDEI	CTNAG	9 cut(s)	22, 332, 469, 636, 705, 933, 1200, 1493, 1682
BstDSI	CCRYGG	3 cut(s)	893, 1549, 1773
BstF5I	GGATG	8 cut(s)	300, 964, 1000, 1086, 1157, 1183, 1231, 1246
BstFNI	CGCG	2 cut(s)	895, 1064
BstH2I	RGCGCY	1 cut(s)	117
BstHHI	GCGC	1 cut(s)	116
BstKTI	GATC	7 cut(s)	175, 739, 833, 1096, 1192, 1492, 1506
BstMAI	GTCTC	1 cut(s)	267
BstMBI	GATC	7 cut(s)	172, 736, 830, 1093, 1189, 1489, 1503
BstMWI	GCNNNNNNNGC	4 cut(s)	232, 383, 1061, 1474
BstNI	CCWGG	4 cut(s)	291, 496, 1299, 1449
BstSCI	CCNGG	7 cut(s)	14, 155, 289, 494, 1089, 1297, 1447
BstSFI	CTRYAG	1 cut(s)	1542
BstSLI	GKGCMC	2 cut(s)	502, 1442
BstUI	CGCG	2 cut(s)	895, 1064
BstV1I	GCAGC	4 cut(s)	389, 789, 1556, 1642
BsuRI	GGCC	5 cut(s)	5, 500, 864, 1573, 1627
BtgI	CCRYGG	3 cut(s)	893, 1549, 1773
BtsCI	GGATG	8 cut(s)	300, 964, 1000, 1086, 1157, 1183, 1231, 1246
BtsI	GCAGTG	3 cut(s)	724, 1506, 1561
BtsIMutI	CAGTG	7 cut(s)	657, 724, 821, 1317, 1464, 1506, 1561
Cac8I	GCNNGC	3 cut(s)	567, 1479, 1707
CaiI	CAGNNNCTG	1 cut(s)	1298
CciI	TCATGA	1 cut(s)	78
CfoI	GCGC	1 cut(s)	116
Cfr13I	GGNCC	4 cut(s)	399, 498, 499, 587
Cfr42I	CCGCGG	1 cut(s)	896
CseI	GACGC	2 cut(s)	1053, 1859
Csp6I	GTAC	6 cut(s)	160, 742, 912, 1372, 1610, 1644
CspCI	CAANNNNNGTGG	2 cut(s)	700, 735
CviAII	CATG	8 cut(s)	79, 570, 1052, 1112, 1487, 1532, 1774, 1786
CviQI	GTAC	6 cut(s)	160, 742, 912, 1372, 1610, 1644
DdeI	CTNAG	9 cut(s)	22, 332, 469, 636, 705, 933, 1200, 1493, 1682
DinI	GGCGCC	1 cut(s)	115
DpnI	GATC	7 cut(s)	174, 738, 832, 1095, 1191, 1491, 1505
DpnII	GATC	7 cut(s)	172, 736, 830, 1093, 1189, 1489, 1503
EaeI	YGGCCR	1 cut(s)	862
Eam1104I	CTCTTC	5 cut(s)	16, 274, 343, 614, 670
EarI	CTCTTC	5 cut(s)	16, 274, 343, 614, 670
Ecl136II	GAGCTC	1 cut(s)	1197
Eco130I	CCWWGG	3 cut(s)	1421, 1773, 1830
Eco147I	AGGCCT	2 cut(s)	1573, 1627
Eco24I	GRGCYC	5 cut(s)	302, 502, 1109, 1199, 1739
Eco47I	GGWCC	2 cut(s)	399, 587
Eco53kI	GAGCTC	1 cut(s)	1197
Eco57I	CTGAAG	2 cut(s)	597, 633
Eco88I	CYCGRG	3 cut(s)	46, 109, 1721
EcoICRI	GAGCTC	1 cut(s)	1197
EcoO109I	RGGNCCY	3 cut(s)	498, 499, 587
EcoRI	GAATTC	1 cut(s)	1153
EcoRII	CCWGG	4 cut(s)	289, 494, 1297, 1447
EcoT14I	CCWWGG	3 cut(s)	1421, 1773, 1830
EcoT38I	GRGCYC	5 cut(s)	302, 502, 1109, 1199, 1739
EgeI	GGCGCC	1 cut(s)	115
EheI	GGCGCC	1 cut(s)	115
ErhI	CCWWGG	3 cut(s)	1421, 1773, 1830
FaeI	CATG	8 cut(s)	82, 573, 1055, 1115, 1490, 1535, 1777, 1789
FaqI	GGGAC	3 cut(s)	188, 202, 691
FatI	CATG	8 cut(s)	78, 569, 1051, 1111, 1486, 1531, 1773, 1785
FauI	CCCGC	2 cut(s)	231, 310
FbaI	TGATCA	2 cut(s)	1189, 1503
Fnu4HI	GCNGC	5 cut(s)	378, 803, 1041, 1545, 1656
FokI	GGATG	8 cut(s)	307, 971, 987, 1073, 1144, 1190, 1238, 1253
FriOI	GRGCYC	5 cut(s)	302, 502, 1109, 1199, 1739
Fsp4HI	GCNGC	5 cut(s)	378, 803, 1041, 1545, 1656
FspBI	CTAG	2 cut(s)	584, 1020
GlaI	GCGC	1 cut(s)	115
GluI	GCNGC	5 cut(s)	378, 803, 1041, 1545, 1656
GsaI	CCCAGC	2 cut(s)	235, 1742
HaeII	RGCGCY	1 cut(s)	117
HaeIII	GGCC	5 cut(s)	5, 500, 864, 1573, 1627
HapII	CCGG	5 cut(s)	15, 156, 439, 1091, 1148
HgaI	GACGC	2 cut(s)	1053, 1859
HhaI	GCGC	1 cut(s)	116
Hin1I	GRCGYC	1 cut(s)	114
Hin1II	CATG	8 cut(s)	82, 573, 1055, 1115, 1490, 1535, 1777, 1789
Hin6I	GCGC	1 cut(s)	114
HinP1I	GCGC	1 cut(s)	114
HinfI	GANTC	2 cut(s)	317, 1862
HpaII	CCGG	5 cut(s)	15, 156, 439, 1091, 1148
HphI	GGTGA	5 cut(s)	35, 49, 640, 961, 1286
Hpy166II	GTNNAC	3 cut(s)	912, 982, 1440
Hpy188III	TCNNGA	8 cut(s)	79, 314, 946, 1215, 1361, 1673, 1721, 1723
Hpy8I	GTNNAC	3 cut(s)	912, 982, 1440
HpyAV	CCTTC	7 cut(s)	70, 100, 871, 1017, 1265, 1511, 1694
HpyCH4III	ACNGT	2 cut(s)	1312, 1643
HpyCH4IV	ACGT	1 cut(s)	1470
HpyF10VI	GCNNNNNNNGC	4 cut(s)	232, 383, 1061, 1474
HpyF3I	CTNAG	9 cut(s)	22, 332, 469, 636, 705, 933, 1200, 1493, 1682
HpySE526I	ACGT	1 cut(s)	1470
Hsp92I	GRCGYC	1 cut(s)	114
Hsp92II	CATG	8 cut(s)	82, 573, 1055, 1115, 1490, 1535, 1777, 1789
HspAI	GCGC	1 cut(s)	114
KasI	GGCGCC	1 cut(s)	113
Ksp22I	TGATCA	2 cut(s)	1189, 1503
KspI	CCGCGG	1 cut(s)	896
Kzo9I	GATC	7 cut(s)	172, 736, 830, 1093, 1189, 1489, 1503
LguI	GCTCTTC	1 cut(s)	343
LmnI	GCTCC	6 cut(s)	18, 184, 240, 454, 897, 1425
Lsp1109I	GCAGC	4 cut(s)	389, 789, 1556, 1642
MaeI	CTAG	2 cut(s)	584, 1020
MaeII	ACGT	1 cut(s)	1470
MaeIII	GTNAC	2 cut(s)	1274, 1346
MalI	GATC	7 cut(s)	174, 738, 832, 1095, 1191, 1491, 1505
MboI	GATC	7 cut(s)	172, 736, 830, 1093, 1189, 1489, 1503
MboII	GAAGA	9 cut(s)	3, 261, 330, 341, 603, 631, 639, 657, 1038
MfeI	CAATTG	1 cut(s)	847
MhlI	GDGCHC	7 cut(s)	302, 459, 502, 1109, 1199, 1442, 1739
MlsI	TGGCCA	1 cut(s)	864
MluI	ACGCGT	1 cut(s)	1062
MluNI	TGGCCA	1 cut(s)	864
Mly113I	GGCGCC	1 cut(s)	114
MlyI	GAGTC	1 cut(s)	311
MmeI	TCCRAC	5 cut(s)	120, 366, 383, 530, 936
Mox20I	TGGCCA	1 cut(s)	864
MscI	TGGCCA	1 cut(s)	864
MseI	TTAA	2 cut(s)	390, 447
MslI	CAYNNNNRTG	3 cut(s)	1530, 1619, 1870
Msp20I	TGGCCA	1 cut(s)	864
MspA1I	CMGCKG	3 cut(s)	802, 895, 1040
MspI	CCGG	5 cut(s)	15, 156, 439, 1091, 1148
MspR9I	CCNGG	7 cut(s)	16, 157, 291, 496, 1091, 1299, 1449
MunI	CAATTG	1 cut(s)	847
MvaI	CCWGG	4 cut(s)	291, 496, 1299, 1449
MvnI	CGCG	2 cut(s)	895, 1064
MwoI	GCNNNNNNNGC	4 cut(s)	232, 383, 1061, 1474
NarI	GGCGCC	1 cut(s)	114
NciI	CCSGG	3 cut(s)	16, 157, 1091
NcoI	CCATGG	1 cut(s)	1773
NdeII	GATC	7 cut(s)	172, 736, 830, 1093, 1189, 1489, 1503
NlaIII	CATG	8 cut(s)	82, 573, 1055, 1115, 1490, 1535, 1777, 1789
NlaIV	GGNNCC	3 cut(s)	115, 500, 501
NmeAIII	GCCGAG	1 cut(s)	331
NmuCI	GTSAC	2 cut(s)	1274, 1346
PaeR7I	CTCGAG	1 cut(s)	1721
PagI	TCATGA	1 cut(s)	78
PceI	AGGCCT	2 cut(s)	1573, 1627
PciSI	GCTCTTC	1 cut(s)	343
PfeI	GAWTC	1 cut(s)	1862
PflMI	CCANNNNNTGG	3 cut(s)	365, 393, 1260
PfoI	TCCNGGA	2 cut(s)	14, 1089
PkrI	GCNGC	5 cut(s)	379, 804, 1042, 1546, 1657
PleI	GAGTC	1 cut(s)	311
PluTI	GGCGCC	1 cut(s)	117
PpsI	GAGTC	1 cut(s)	311
Ppu21I	YACGTR	1 cut(s)	1471
PpuMI	RGGWCCY	1 cut(s)	587
PshBI	ATTAAT	1 cut(s)	390
Psp124BI	GAGCTC	1 cut(s)	1199
Psp5II	RGGWCCY	1 cut(s)	587
Psp6I	CCWGG	4 cut(s)	289, 494, 1297, 1447
PspFI	CCCAGC	2 cut(s)	231, 1738
PspGI	CCWGG	4 cut(s)	289, 494, 1297, 1447
PspN4I	GGNNCC	3 cut(s)	115, 500, 501
PspOMI	GGGCCC	1 cut(s)	498
PspPI	GGNCC	4 cut(s)	399, 498, 499, 587
PspPPI	RGGWCCY	1 cut(s)	587
PstI	CTGCAG	1 cut(s)	1546
PstNI	CAGNNNCTG	1 cut(s)	1298
PvuII	CAGCTG	1 cut(s)	802
RsaI	GTAC	6 cut(s)	161, 743, 913, 1373, 1611, 1645
RsaNI	GTAC	6 cut(s)	160, 742, 912, 1372, 1610, 1644
RseI	CAYNNNNRTG	3 cut(s)	1530, 1619, 1870
SacI	GAGCTC	1 cut(s)	1199
SacII	CCGCGG	1 cut(s)	896
SapI	GCTCTTC	1 cut(s)	343
SaqAI	TTAA	2 cut(s)	390, 447
SatI	GCNGC	5 cut(s)	378, 803, 1041, 1545, 1656
Sau3AI	GATC	7 cut(s)	172, 736, 830, 1093, 1189, 1489, 1503
Sau96I	GGNCC	4 cut(s)	399, 498, 499, 587
ScaI	AGTACT	1 cut(s)	1645
SchI	GAGTC	1 cut(s)	311
ScrFI	CCNGG	7 cut(s)	16, 157, 291, 496, 1091, 1299, 1449
SduI	GDGCHC	7 cut(s)	302, 459, 502, 1109, 1199, 1442, 1739
SfcI	CTRYAG	1 cut(s)	1542
SfoI	GGCGCC	1 cut(s)	115
Sfr274I	CTCGAG	1 cut(s)	1721
Sfr303I	CCGCGG	1 cut(s)	896
SgrBI	CCGCGG	1 cut(s)	896
SinI	GGWCC	2 cut(s)	399, 587
SlaI	CTCGAG	1 cut(s)	1721
SmiMI	CAYNNNNRTG	3 cut(s)	1530, 1619, 1870
SmlI	CTYRAG	2 cut(s)	381, 1721
SmoI	CTYRAG	2 cut(s)	381, 1721
SseBI	AGGCCT	2 cut(s)	1573, 1627
SsiI	CCGC	8 cut(s)	33, 141, 182, 224, 303, 893, 895, 1040
SspDI	GGCGCC	1 cut(s)	113
SspMI	CTAG	2 cut(s)	584, 1020
SstI	GAGCTC	1 cut(s)	1199
StuI	AGGCCT	2 cut(s)	1573, 1627
StyD4I	CCNGG	7 cut(s)	14, 155, 289, 494, 1089, 1297, 1447
StyI	CCWWGG	3 cut(s)	1421, 1773, 1830
TaaI	ACNGT	2 cut(s)	1312, 1643
TaiI	ACGT	1 cut(s)	1473
TaqI	TCGA	3 cut(s)	315, 945, 1722
TatI	WGTACW	3 cut(s)	741, 911, 1643
TauI	GCSGC	1 cut(s)	1043
TfiI	GAWTC	1 cut(s)	1862
Tru1I	TTAA	2 cut(s)	390, 447
Tru9I	TTAA	2 cut(s)	390, 447
TscAI	CASTG	7 cut(s)	657, 724, 821, 1317, 1464, 1506, 1561
TseFI	GTSAC	2 cut(s)	1274, 1346
TseI	GCWGC	4 cut(s)	377, 802, 1544, 1655
Tsp45I	GTSAC	2 cut(s)	1274, 1346
TspDTI	ATGAA	6 cut(s)	67, 501, 1023, 1146, 1296, 1802
TspRI	CASTG	7 cut(s)	657, 724, 821, 1317, 1464, 1506, 1561
Van91I	CCANNNNNTGG	3 cut(s)	365, 393, 1260
VneI	GTGCAC	1 cut(s)	1438
VpaK11BI	GGWCC	2 cut(s)	399, 587
VspI	ATTAAT	1 cut(s)	390
XapI	RAATTY	2 cut(s)	37, 1153
XhoI	CTCGAG	1 cut(s)	1721
XspI	CTAG	2 cut(s)	584, 1020
ZrmI	AGTACT	1 cut(s)	1645

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.