Rw3G009250
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0001456
Physical Location & Seq
Forward (+)
8850973 .. 8853230
2258 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G009250.1

Sequence Viewer

Length: 1257 bp
ATGTCTTCTTCCTTCACTCACCTCCTCACAAGCAACATGAACATGAACAATTTTGACCAGGACCGAACTGGCAGCTGGAGCTGGGGACTCTCCGACCGTTCAACGGAAGCCGAAATCCCCAAGTTCAAGTCACTCCAGCCGCCTTCTCTCCCTCTCTCTCCTCCTCCTGCTTCCCCTTCTTCTTACTTGGTTAACAACCCTGGTTTTAGCCCTACTGATTTTTTCAGCTCACCCATGTTTCTTACTCACTCAAATACTCTTCCATCTCCGACCACCGGAGCCTTTTCGAGTCAGATTTTCGATTGGATGAGCACTCCTAAAGAAAATAACAGCCAGCATGGAGCGGAGAAGGAGCAGAAAATGTTCGCTGATTTCTCATTTCAACCGGCAACATCGTCTTCATCGTTCAGTGTTCAGCAATCTTCTTCAAGCATGGTTTCATCGGTGGAGGAATCATTGAAAAGTCAACAGCAGCAATCGTGGGGTTTCAACAACCAAAGGCAAGAAGAAAGCGTAGCAGAGAAAACAGAAGTGAAATCCGAGTTTGCGCCAACTCAGAGCTTCAATGCTGTTCCAAAGTCTGATTACACTCATTCGACCCAATATGTGAGGGAACAGAAATCAGATGATGGATACAATTGGAGAAAATATGGACAAAAGCAAGTGAAAGGTAGCGAAAATCCGCGGAGTTATTACAAGTGTACATTTCCCAGTTGCCCAACAAAGAAGAAGGTGGAGAGATCATTGGATGGACAGATCACTCAAATTGTGTACAAGGGAAGTCACAACCATGCTAAGCCTCAGTCGACAAGACGATCAGCCTCTCAACAACAATCGCTTCAAACAAGTTCGTATGGGATTTCTGATCAGTCTGCCTCGACAATATCCAATCCAAAAATCGAAGCTGTCACGCTGCAGGAGGACTCTAACTCAGCCTCAATGGGAGGAGAGGATGAGTTTGAACAAAATTCGCCGATAAGTAACTCAAATGGAGCTGATGATGACAATGAGCCTGAGGCCAAAAGATGGAAGGGAGAAAATGCAAATGAGACGTATGCAGCTCCTGGGAGTAGAATTGTGAAAGAACCTAGAATTGTAGTGCAGACCACAAGTGAGATTGATATTCTGGATGATGGGTATAGATGGAGGAAATATGGACAGAAAGTAGTGAAGGGAAATCCAAACCCAAGGTGCATACACATTTTGAAACCCTGTAATCATGAGTTCATACATTATGAATTTCTACTGCTTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
WRKY

Protein Analysis

418

Amino Acids

46.83

Weight (kDa)

6.04

Isoelectric Point (pI)

65.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 209 - 264 5.5e-26 WRKY DNA -binding domain
WRKY PF03106 377 - 398 2.8e-07 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1026
AccBSI CCGCTC 1 cut(s) 344
AccI GTMKAC 1 cut(s) 806
AccII CGCG 1 cut(s) 685
AciI CCGC 4 cut(s) 140, 344, 683, 685
AcsI RAATTY 2 cut(s) 967, 1238
AfaI GTAC 2 cut(s) 703, 773
AfiI CCNNNNNNNGG 3 cut(s) 103, 275, 1026
AjnI CCWGG 3 cut(s) 57, 199, 1063
AluBI AGCT 7 cut(s) 75, 81, 228, 561, 905, 995, 1061
AluI AGCT 7 cut(s) 75, 81, 228, 561, 905, 995, 1061
Alw21I GWGCWC 1 cut(s) 314
Alw26I GTCTC 1 cut(s) 1043
AlwNI CAGNNNCTG 1 cut(s) 1064
AoxI GGCC 1 cut(s) 1017
ApeKI GCWGC 4 cut(s) 72, 472, 913, 1058
ApoI RAATTY 2 cut(s) 967, 1238
Asp700I GAANNNNTTC 1 cut(s) 362
AspLEI GCGC 1 cut(s) 550
AspS9I GGNCC 1 cut(s) 61
AsuHPI GGTGA 2 cut(s) 11, 222
AvaII GGWCC 1 cut(s) 61
AxyI CCTNAGG 1 cut(s) 1014
BbsI GAAGAC 1 cut(s) 390
Bbv12I GWGCWC 1 cut(s) 314
BbvI GCAGC 4 cut(s) 84, 484, 900, 1070
BccI CCATC 6 cut(s) 271, 623, 743, 1020, 1127, 1137
BciT130I CCWGG 3 cut(s) 59, 201, 1065
BciVI GTATCC 1 cut(s) 626
BclI TGATCA 1 cut(s) 865
BcoDI GTCTC 1 cut(s) 1043
BfaI CTAG 1 cut(s) 1089
BfmI CTRYAG 1 cut(s) 914
BfuI GTATCC 1 cut(s) 626
BisI GCNGC 5 cut(s) 73, 140, 473, 914, 1059
BlpI GCTNAGC 1 cut(s) 795
BlsI GCNGC 5 cut(s) 74, 141, 474, 915, 1060
Bme1390I CCNGG 3 cut(s) 59, 201, 1065
Bme18I GGWCC 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 61
BmiI GGNNCC 1 cut(s) 280
BmrFI CCNGG 3 cut(s) 59, 201, 1065
BmrI ACTGGG 1 cut(s) 705
BmuI ACTGGG 1 cut(s) 705
BpiI GAAGAC 1 cut(s) 390
BpmI CTGGAG 2 cut(s) 97, 119
Bpu1102I GCTNAGC 1 cut(s) 795
BsaJI CCNNGG 4 cut(s) 199, 683, 1064, 1187
BsaWI WCCGGW 1 cut(s) 275
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 275, 1026
Bse118I RCCGGY 1 cut(s) 385
Bse1I ACTGG 2 cut(s) 73, 711
Bse21I CCTNAGG 1 cut(s) 1014
BseBI CCWGG 3 cut(s) 59, 201, 1065
BseDI CCNNGG 4 cut(s) 199, 683, 1064, 1187
BseGI GGATG 4 cut(s) 312, 754, 958, 1135
BseLI CCNNNNNNNGG 3 cut(s) 103, 275, 1026
BseMII CTCAG 4 cut(s) 569, 815, 945, 1005
BseNI ACTGG 2 cut(s) 73, 711
BseRI GAGGAG 4 cut(s) 14, 150, 153, 960
BseXI GCAGC 4 cut(s) 84, 484, 900, 1070
BseYI CCCAGC 1 cut(s) 81
BsgI GTGCAG 1 cut(s) 1121
Bsh1236I CGCG 1 cut(s) 685
Bsh1285I CGRYCG 1 cut(s) 97
BshFI GGCC 1 cut(s) 1019
BsiEI CGRYCG 1 cut(s) 97
BsiHKAI GWGCWC 1 cut(s) 314
BsiSI CCGG 2 cut(s) 276, 386
BslFI GGGAC 1 cut(s) 99
BslI CCNNNNNNNGG 3 cut(s) 103, 275, 1026
BsmAI GTCTC 1 cut(s) 1043
BsmBI CGTCTC 1 cut(s) 1043
BsmFI GGGAC 1 cut(s) 99
BsnI GGCC 1 cut(s) 1019
Bsp1286I GDGCHC 1 cut(s) 314
Bsp1407I TGTACA 2 cut(s) 701, 771
Bsp143I GATC 4 cut(s) 740, 756, 815, 865
Bsp1720I GCTNAGC 1 cut(s) 795
BspACI CCGC 4 cut(s) 140, 344, 683, 685
BspANI GGCC 1 cut(s) 1019
BspCNI CTCAG 4 cut(s) 568, 814, 944, 1006
BspFNI CGCG 1 cut(s) 685
BspHI TCATGA 1 cut(s) 1219
BspLI GGNNCC 1 cut(s) 280
BspMAI CTGCAG 1 cut(s) 918
BsrBI CCGCTC 1 cut(s) 344
BsrFI RCCGGY 1 cut(s) 385
BsrGI TGTACA 2 cut(s) 701, 771
BsrI ACTGG 2 cut(s) 73, 711
BssAI RCCGGY 1 cut(s) 385
BssECI CCNNGG 4 cut(s) 199, 683, 1064, 1187
BssMI GATC 4 cut(s) 740, 756, 815, 865
BssT1I CCWWGG 1 cut(s) 1187
Bst2UI CCWGG 3 cut(s) 59, 201, 1065
Bst4CI ACNGT 1 cut(s) 98
Bst6I CTCTTC 1 cut(s) 264
BstAUI TGTACA 2 cut(s) 701, 771
BstC8I GCNNGC 1 cut(s) 335
BstDEI CTNAG 5 cut(s) 555, 795, 801, 931, 1014
BstDSI CCRYGG 1 cut(s) 683
BstF5I GGATG 4 cut(s) 312, 754, 958, 1135
BstFNI CGCG 1 cut(s) 685
BstHHI GCGC 1 cut(s) 550
BstKTI GATC 4 cut(s) 743, 759, 818, 868
BstMAI GTCTC 1 cut(s) 1043
BstMBI GATC 4 cut(s) 740, 756, 815, 865
BstMCI CGRYCG 1 cut(s) 97
BstMWI GCNNNNNNNGC 1 cut(s) 78
BstNI CCWGG 3 cut(s) 59, 201, 1065
BstSCI CCNGG 3 cut(s) 57, 199, 1063
BstSFI CTRYAG 1 cut(s) 914
BstUI CGCG 1 cut(s) 685
BstV1I GCAGC 4 cut(s) 84, 484, 900, 1070
BstV2I GAAGAC 1 cut(s) 390
Bsu36I CCTNAGG 1 cut(s) 1014
BsuI GTATCC 1 cut(s) 626
BsuRI GGCC 1 cut(s) 1019
BtgI CCRYGG 1 cut(s) 683
BtsCI GGATG 4 cut(s) 312, 754, 958, 1135
BtsIMutI CAGTG 1 cut(s) 415
Cac8I GCNNGC 1 cut(s) 335
CaiI CAGNNNCTG 1 cut(s) 1064
CciI TCATGA 1 cut(s) 1219
CfoI GCGC 1 cut(s) 550
Cfr10I RCCGGY 1 cut(s) 385
Cfr13I GGNCC 1 cut(s) 61
Cfr42I CCGCGG 1 cut(s) 686
Csp6I GTAC 2 cut(s) 702, 772
CviAII CATG 7 cut(s) 37, 43, 235, 338, 433, 791, 1220
CviQI GTAC 2 cut(s) 702, 772
DdeI CTNAG 5 cut(s) 555, 795, 801, 931, 1014
DpnI GATC 4 cut(s) 742, 758, 817, 867
DpnII GATC 4 cut(s) 740, 756, 815, 865
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
Eco130I CCWWGG 1 cut(s) 1187
Eco47I GGWCC 1 cut(s) 61
Eco81I CCTNAGG 1 cut(s) 1014
EcoRII CCWGG 3 cut(s) 57, 199, 1063
EcoT14I CCWWGG 1 cut(s) 1187
ErhI CCWWGG 1 cut(s) 1187
Esp3I CGTCTC 1 cut(s) 1043
FaeI CATG 7 cut(s) 40, 46, 238, 341, 436, 794, 1223
FaqI GGGAC 1 cut(s) 99
FatI CATG 7 cut(s) 36, 42, 234, 337, 432, 790, 1219
FbaI TGATCA 1 cut(s) 865
FblI GTMKAC 1 cut(s) 806
Fnu4HI GCNGC 5 cut(s) 73, 140, 473, 914, 1059
FokI GGATG 4 cut(s) 319, 761, 965, 1142
Fsp4HI GCNGC 5 cut(s) 73, 140, 473, 914, 1059
FspBI CTAG 1 cut(s) 1089
GlaI GCGC 1 cut(s) 549
GluI GCNGC 5 cut(s) 73, 140, 473, 914, 1059
GsaI CCCAGC 1 cut(s) 85
GsuI CTGGAG 2 cut(s) 97, 119
HaeIII GGCC 1 cut(s) 1019
HapII CCGG 2 cut(s) 276, 386
HhaI GCGC 1 cut(s) 550
Hin1II CATG 7 cut(s) 40, 46, 238, 341, 436, 794, 1223
Hin6I GCGC 1 cut(s) 548
HinP1I GCGC 1 cut(s) 548
HincII GTYRAC 3 cut(s) 193, 467, 807
HindII GTYRAC 3 cut(s) 193, 467, 807
HinfI GANTC 4 cut(s) 87, 289, 452, 923
HpaI GTTAAC 1 cut(s) 193
HpaII CCGG 2 cut(s) 276, 386
HphI GGTGA 2 cut(s) 11, 222
Hpy166II GTNNAC 5 cut(s) 193, 467, 702, 772, 807
Hpy188I TCNGA 8 cut(s) 94, 270, 294, 541, 558, 583, 625, 865
Hpy188III TCNNGA 2 cut(s) 1127, 1220
Hpy8I GTNNAC 5 cut(s) 193, 467, 702, 772, 807
HpyAV CCTTC 7 cut(s) 22, 153, 186, 343, 724, 1024, 1165
HpyCH4III ACNGT 1 cut(s) 98
HpyCH4IV ACGT 1 cut(s) 1052
HpyCH4V TGCA 5 cut(s) 916, 1043, 1058, 1102, 1194
HpyF10VI GCNNNNNNNGC 1 cut(s) 78
HpyF3I CTNAG 5 cut(s) 555, 795, 801, 931, 1014
HpySE526I ACGT 1 cut(s) 1052
Hsp92II CATG 7 cut(s) 40, 46, 238, 341, 436, 794, 1223
HspAI GCGC 1 cut(s) 548
Ksp22I TGATCA 1 cut(s) 865
KspAI GTTAAC 1 cut(s) 193
KspI CCGCGG 1 cut(s) 686
Kzo9I GATC 4 cut(s) 740, 756, 815, 865
LmnI GCTCC 6 cut(s) 78, 278, 341, 352, 992, 1066
Lsp1109I GCAGC 4 cut(s) 84, 484, 900, 1070
MaeI CTAG 1 cut(s) 1089
MaeII ACGT 1 cut(s) 1052
MaeIII GTNAC 4 cut(s) 129, 782, 907, 980
MalI GATC 4 cut(s) 742, 758, 817, 867
MbiI CCGCTC 1 cut(s) 344
MboI GATC 4 cut(s) 740, 756, 815, 865
MboII GAAGA 7 cut(s) 171, 251, 390, 414, 417, 518, 739
MfeI CAATTG 1 cut(s) 637
MhlI GDGCHC 1 cut(s) 314
MluCI AATT 7 cut(s) 49, 637, 765, 967, 1074, 1092, 1238
MlyI GAGTC 3 cut(s) 81, 298, 917
MmeI TCCRAC 2 cut(s) 117, 293
MroXI GAANNNNTTC 1 cut(s) 362
MseI TTAA 2 cut(s) 192, 1251
MslI CAYNNNNRTG 2 cut(s) 41, 789
MspA1I CMGCKG 2 cut(s) 75, 685
MspI CCGG 2 cut(s) 276, 386
MspR9I CCNGG 3 cut(s) 59, 201, 1065
MunI CAATTG 1 cut(s) 637
MvaI CCWGG 3 cut(s) 59, 201, 1065
MvnI CGCG 1 cut(s) 685
MwoI GCNNNNNNNGC 1 cut(s) 78
NdeII GATC 4 cut(s) 740, 756, 815, 865
NlaIII CATG 7 cut(s) 40, 46, 238, 341, 436, 794, 1223
NlaIV GGNNCC 1 cut(s) 280
NmuCI GTSAC 3 cut(s) 129, 782, 907
PagI TCATGA 1 cut(s) 1219
PcsI WCGNNNNNNNCGW 1 cut(s) 401
PdmI GAANNNNTTC 1 cut(s) 362
PfeI GAWTC 1 cut(s) 452
PflMI CCANNNNNTGG 1 cut(s) 1026
PkrI GCNGC 5 cut(s) 74, 141, 474, 915, 1060
PleI GAGTC 3 cut(s) 81, 297, 917
PpsI GAGTC 3 cut(s) 81, 297, 917
Psp6I CCWGG 3 cut(s) 57, 199, 1063
PspFI CCCAGC 1 cut(s) 81
PspGI CCWGG 3 cut(s) 57, 199, 1063
PspN4I GGNNCC 1 cut(s) 280
PspPI GGNCC 1 cut(s) 61
PstI CTGCAG 1 cut(s) 918
PstNI CAGNNNCTG 1 cut(s) 1064
PvuII CAGCTG 1 cut(s) 75
RsaI GTAC 2 cut(s) 703, 773
RsaNI GTAC 2 cut(s) 702, 772
RseI CAYNNNNRTG 2 cut(s) 41, 789
SacII CCGCGG 1 cut(s) 686
SalI GTCGAC 1 cut(s) 805
SaqAI TTAA 2 cut(s) 192, 1251
SatI GCNGC 5 cut(s) 73, 140, 473, 914, 1059
Sau3AI GATC 4 cut(s) 740, 756, 815, 865
Sau96I GGNCC 1 cut(s) 61
SchI GAGTC 3 cut(s) 81, 298, 917
ScrFI CCNGG 3 cut(s) 59, 201, 1065
SduI GDGCHC 1 cut(s) 314
SfcI CTRYAG 1 cut(s) 914
Sfr303I CCGCGG 1 cut(s) 686
SgrBI CCGCGG 1 cut(s) 686
SinI GGWCC 1 cut(s) 61
SmiMI CAYNNNNRTG 2 cut(s) 41, 789
Sse9I AATT 7 cut(s) 49, 637, 765, 967, 1074, 1092, 1238
SsiI CCGC 4 cut(s) 140, 344, 683, 685
SspMI CTAG 1 cut(s) 1089
StyD4I CCNGG 3 cut(s) 57, 199, 1063
StyI CCWWGG 1 cut(s) 1187
TaaI ACNGT 1 cut(s) 98
TaiI ACGT 1 cut(s) 1055
TaqI TCGA 6 cut(s) 287, 300, 596, 806, 878, 900
TaqII GACCGA 1 cut(s) 78
TasI AATT 7 cut(s) 49, 637, 765, 967, 1074, 1092, 1238
TatI WGTACW 2 cut(s) 701, 771
TauI GCSGC 1 cut(s) 142
TfiI GAWTC 1 cut(s) 452
Tru1I TTAA 2 cut(s) 192, 1251
Tru9I TTAA 2 cut(s) 192, 1251
TscAI CASTG 1 cut(s) 415
TseFI GTSAC 3 cut(s) 129, 782, 907
TseI GCWGC 4 cut(s) 72, 472, 913, 1058
Tsp45I GTSAC 3 cut(s) 129, 782, 907
TspDTI ATGAA 6 cut(s) 53, 59, 390, 429, 1216, 1251
TspGWI ACGGA 1 cut(s) 119
TspRI CASTG 1 cut(s) 415
Van91I CCANNNNNTGG 1 cut(s) 1026
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 2 cut(s) 967, 1238
XcmI CCANNNNNNNNNTGG 1 cut(s) 65
XmiI GTMKAC 1 cut(s) 806
XmnI GAANNNNTTC 1 cut(s) 362
XspI CTAG 1 cut(s) 1089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.