pycom12g17060
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001456
Physical Location & Seq
Reverse (-)
19329619 .. 19331614
1996 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g17060.5

Sequence Viewer

Length: 1533 bp
ATGACTTCTTCCTTCACTCACCTCCTCACAAGCAACATGGAGAACAGCGGGATTGGACAGGACAGGACTAATTGGGGGGGACTATTCTCGGACTATTCTTCTAATCCGATCCGGTTCACGGACAGAAACGGGACTGACATCCCCAAGTTCAAGTCACTCCAGCCTCCTTCTCTGCCTCTCTCTCCTCCCCCTGTTTCCCCTTCTTCTTACTTGGCTTCCACGCCGGCTTTTAGTCCCGCCGATTTTCTCAGCTCACCCATGTTCCTCTCATATAATCTTGAATCTCCAACAACAGGAGCTTTTTCGAGTCAGGTTTTCGATTGGATGAATGATACTAAAGACACCCAGCAAGGAATTAAGGATAGAGAGGAGCCGAAAATGTTCTCCGATTTCTCGTTCCAACCCGAATCGAGGCCTGCTACTAATCTTCAATCTTCTTCTAGCATGGTTTCAGTGGAAGAACCATTCAAAAGAGAAAGGCAAGCATGGGATTTTAATGCTGAGAAGGCGGGAGTGAAGTCCGAATTCGAGCCAATCGAAGCGAATACGCAGAGTAATGAACTTAATGGTGCTCCAAAGTCCGATTACTTGCATTCTAATCAATCTTCGCAATATGCCCGAGAACAAAAATCGGATGATGGGTTCAATTGGAGGAAGTATGGGCAGAAACAAGTGAAGGGTAGCGAAAATCCGCGGAGTTATTACAAGTGCACGTTTCCGAATTGCCCCACAAAGAAGAAGGTTGAGAGATCATTGGATGGACAGATTACTCAAATTGTGTACAAGGGTAATCACAACCATCATAAGCCTCAGTCTACAAGAAGATCAAGCTCTAACTCGATTCAGGGTTCTTTCTATGGAATCTCTGATCAATCCGTTCCGACGTTGTCCAATCCGAAAGTTGAGTCCGTCTCACTACAGGAGGATTCTTCTACCTCAATTGGAGAGGATGAGTTTGAACAGAACTCTCCAATAAGTAATTCAGTAGGAGCTGAAGATGAAAACGAACCTGAGGCGAAAAGATGGAAGGGAGACAATGCAAATGATCAGTCATATGCATCTTCTGGCGGTAGAATTGTGAAAGAACCGAGAATTGTAGTGCAGACAACGAGCGAAATCGATATTCTGGATGACGGGTATAGGTGGAGGAAATATGGACAGAAAGTAGTGAAGGGAAATCCAAATCCAAGGAGCTACTACAAATGCACGTCCGTAGGTTGTCCAGTGAGGAAACATGTGGAGAGAGCATCGCAGGACACAAGGGCGGTGATCACCACGTACGAAGGGAAGCACAACCACGATGTTCCGGCTGCGCGAGGCAGCGGGAATTATAGCAATGCAAGTAGACCTACTGCCGATAATAGCAGCAACAATGTGTCCATGGCTGTGAGGCCCTTGGCATTACCTAACCATTCTAATTTGAGCTACCTCAACTCTCTTCAGAACAGAACGCAGCCAGCAACCACTGAAAACCAATCGTACACTCTCAAAATGTTGCAGAGTGCGGGAAGTCATGGATTTTCTGACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
WRKY

Protein Analysis

511

Amino Acids

56.75

Weight (kDa)

6.58

Isoelectric Point (pI)

57.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 815, 1345
AccII CGCG 2 cut(s) 694, 1315
AciI CCGC 9 cut(s) 48, 237, 509, 692, 694, 1068, 1265, 1323, 1505
AclWI GGATC 1 cut(s) 103
AcsI RAATTY 1 cut(s) 524
AcuI CTGAAG 2 cut(s) 1014, 1424
AfaI GTAC 3 cut(s) 782, 1280, 1481
AfiI CCNNNNNNNGG 3 cut(s) 118, 293, 411
AflIII ACRYGT 1 cut(s) 1234
AgsI TTSAA 6 cut(s) 151, 281, 431, 469, 646, 959
AjiI CACGTC 1 cut(s) 1209
AjuI GAANNNNNNNTTGG 2 cut(s) 964, 996
AloI GAACNNNNNNTCC 2 cut(s) 626, 658
AluBI AGCT 6 cut(s) 252, 299, 831, 992, 1194, 1425
AluI AGCT 6 cut(s) 252, 299, 831, 992, 1194, 1425
Alw21I GWGCWC 2 cut(s) 574, 713
Alw26I GTCTC 2 cut(s) 916, 1026
Alw44I GTGCAC 1 cut(s) 709
AlwI GGATC 1 cut(s) 103
Ama87I CYCGRG 1 cut(s) 618
AoxI GGCC 2 cut(s) 413, 1391
ApaLI GTGCAC 1 cut(s) 709
ApeKI GCWGC 4 cut(s) 1310, 1320, 1365, 1453
ApoI RAATTY 1 cut(s) 524
ArsI GACNNNNNNTTYG 4 cut(s) 890, 922, 1034, 1066
Asp700I GAANNNNTTC 1 cut(s) 380
AspLEI GCGC 1 cut(s) 1315
AspS9I GGNCC 1 cut(s) 1392
AsuHPI GGTGA 4 cut(s) 11, 246, 1264, 1279
AvaI CYCGRG 1 cut(s) 618
AxyI CCTNAGG 1 cut(s) 1011
BaeGI GKGCMC 1 cut(s) 713
Bbv12I GWGCWC 2 cut(s) 574, 713
BbvI GCAGC 4 cut(s) 1297, 1332, 1377, 1465
BccI CCATC 4 cut(s) 632, 752, 807, 1017
BclI TGATCA 3 cut(s) 868, 1045, 1269
BcoDI GTCTC 2 cut(s) 916, 1026
BfaI CTAG 1 cut(s) 441
BfmI CTRYAG 1 cut(s) 917
BisI GCNGC 4 cut(s) 1311, 1321, 1366, 1454
BlsI GCNGC 4 cut(s) 1312, 1322, 1367, 1455
BmeT110I CYCGRG 1 cut(s) 618
BmgBI CACGTC 1 cut(s) 1209
BmgT120I GGNCC 1 cut(s) 1392
BmiI GGNNCC 1 cut(s) 372
BmsI GCATC 2 cut(s) 1067, 1256
BplI GAGNNNNNCTC 2 cut(s) 896, 928
BpmI CTGGAG 1 cut(s) 143
Bsa29I ATCGAT 1 cut(s) 1119
BsaAI YACGTR 1 cut(s) 1278
BsaJI CCNNGG 4 cut(s) 692, 1187, 1380, 1395
BsaWI WCCGGW 1 cut(s) 111
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 293, 411
Bse118I RCCGGY 1 cut(s) 223
Bse1I ACTGG 1 cut(s) 1223
Bse21I CCTNAGG 1 cut(s) 1011
Bse3DI GCAATG 1 cut(s) 1342
BseCI ATCGAT 1 cut(s) 1119
BseDI CCNNGG 4 cut(s) 692, 1187, 1380, 1395
BseGI GGATG 6 cut(s) 138, 330, 640, 763, 955, 1135
BseLI CCNNNNNNNGG 3 cut(s) 118, 293, 411
BseMI GCAATG 1 cut(s) 1342
BseMII CTCAG 4 cut(s) 262, 492, 824, 1002
BseNI ACTGG 1 cut(s) 1223
BseRI GAGGAG 3 cut(s) 14, 174, 383
BseSI GKGCMC 1 cut(s) 713
BseXI GCAGC 4 cut(s) 1297, 1332, 1377, 1465
BseYI CCCAGC 1 cut(s) 345
BsgI GTGCAG 1 cut(s) 1121
Bsh1236I CGCG 2 cut(s) 694, 1315
BshFI GGCC 2 cut(s) 415, 1393
BshVI ATCGAT 1 cut(s) 1119
BsiHKAI GWGCWC 2 cut(s) 574, 713
BsiHKCI CYCGRG 1 cut(s) 618
BsiSI CCGG 3 cut(s) 112, 224, 1307
BsiWI CGTACG 1 cut(s) 1278
BslFI GGGAC 3 cut(s) 93, 145, 219
BslI CCNNNNNNNGG 3 cut(s) 118, 293, 411
BsmAI GTCTC 2 cut(s) 916, 1026
BsmBI CGTCTC 1 cut(s) 916
BsmFI GGGAC 3 cut(s) 93, 145, 219
BsmI GAATGC 1 cut(s) 592
BsnI GGCC 2 cut(s) 415, 1393
BsoBI CYCGRG 1 cut(s) 618
Bsp1286I GDGCHC 2 cut(s) 574, 713
Bsp1407I TGTACA 1 cut(s) 780
Bsp143I GATC 6 cut(s) 108, 749, 824, 868, 1045, 1269
Bsp19I CCATGG 1 cut(s) 1380
BspACI CCGC 9 cut(s) 48, 237, 509, 692, 694, 1068, 1265, 1323, 1505
BspANI GGCC 2 cut(s) 415, 1393
BspCNI CTCAG 4 cut(s) 261, 493, 823, 1003
BspDI ATCGAT 1 cut(s) 1119
BspFNI CGCG 2 cut(s) 694, 1315
BspLI GGNNCC 1 cut(s) 372
BspPI GGATC 1 cut(s) 103
BsrDI GCAATG 1 cut(s) 1342
BsrFI RCCGGY 1 cut(s) 223
BsrGI TGTACA 1 cut(s) 780
BsrI ACTGG 1 cut(s) 1223
BssAI RCCGGY 1 cut(s) 223
BssECI CCNNGG 4 cut(s) 692, 1187, 1380, 1395
BssMI GATC 6 cut(s) 108, 749, 824, 868, 1045, 1269
BssT1I CCWWGG 3 cut(s) 1187, 1380, 1395
Bst6I CTCTTC 1 cut(s) 1443
BstAUI TGTACA 1 cut(s) 780
BstBAI YACGTR 1 cut(s) 1278
BstC8I GCNNGC 4 cut(s) 225, 417, 483, 1458
BstDEI CTNAG 4 cut(s) 248, 501, 810, 1011
BstDSI CCRYGG 2 cut(s) 692, 1380
BstF5I GGATG 6 cut(s) 138, 330, 640, 763, 955, 1135
BstFNI CGCG 2 cut(s) 694, 1315
BstHHI GCGC 1 cut(s) 1315
BstKTI GATC 6 cut(s) 111, 752, 827, 871, 1048, 1272
BstMAI GTCTC 2 cut(s) 916, 1026
BstMBI GATC 6 cut(s) 108, 749, 824, 868, 1045, 1269
BstMWI GCNNNNNNNGC 1 cut(s) 506
BstNSI RCATGY 1 cut(s) 1238
BstSFI CTRYAG 1 cut(s) 917
BstSLI GKGCMC 1 cut(s) 713
BstUI CGCG 2 cut(s) 694, 1315
BstV1I GCAGC 4 cut(s) 1297, 1332, 1377, 1465
Bsu15I ATCGAT 1 cut(s) 1119
Bsu36I CCTNAGG 1 cut(s) 1011
BsuRI GGCC 2 cut(s) 415, 1393
BsuTUI ATCGAT 1 cut(s) 1119
BtgI CCRYGG 2 cut(s) 692, 1380
BtgZI GCGATG 1 cut(s) 1233
BtrI CACGTC 1 cut(s) 1209
BtsCI GGATG 6 cut(s) 138, 330, 640, 763, 955, 1135
BtsIMutI CAGTG 3 cut(s) 459, 1230, 1464
Cac8I GCNNGC 4 cut(s) 225, 417, 483, 1458
CfoI GCGC 1 cut(s) 1315
Cfr10I RCCGGY 1 cut(s) 223
Cfr13I GGNCC 1 cut(s) 1392
Cfr42I CCGCGG 1 cut(s) 695
ClaI ATCGAT 1 cut(s) 1119
Csp6I GTAC 3 cut(s) 781, 1279, 1480
CviAII CATG 7 cut(s) 37, 259, 445, 486, 1235, 1381, 1514
CviQI GTAC 3 cut(s) 781, 1279, 1480
DdeI CTNAG 4 cut(s) 248, 501, 810, 1011
DpnI GATC 6 cut(s) 110, 751, 826, 870, 1047, 1271
DpnII GATC 6 cut(s) 108, 749, 824, 868, 1045, 1269
Eam1104I CTCTTC 1 cut(s) 1443
EarI CTCTTC 1 cut(s) 1443
Eco130I CCWWGG 3 cut(s) 1187, 1380, 1395
Eco147I AGGCCT 1 cut(s) 415
Eco57I CTGAAG 2 cut(s) 1014, 1424
Eco81I CCTNAGG 1 cut(s) 1011
Eco88I CYCGRG 1 cut(s) 618
EcoO109I RGGNCCY 1 cut(s) 1392
EcoRI GAATTC 1 cut(s) 524
EcoT14I CCWWGG 3 cut(s) 1187, 1380, 1395
EcoT22I ATGCAT 1 cut(s) 1060
ErhI CCWWGG 3 cut(s) 1187, 1380, 1395
Esp3I CGTCTC 1 cut(s) 916
FaeI CATG 7 cut(s) 40, 262, 448, 489, 1238, 1384, 1517
FaqI GGGAC 3 cut(s) 93, 145, 219
FatI CATG 7 cut(s) 36, 258, 444, 485, 1234, 1380, 1513
FauI CCCGC 5 cut(s) 41, 244, 502, 1316, 1498
FauNDI CATATG 1 cut(s) 1054
FbaI TGATCA 3 cut(s) 868, 1045, 1269
FblI GTMKAC 2 cut(s) 815, 1345
Fnu4HI GCNGC 4 cut(s) 1311, 1321, 1366, 1454
FokI GGATG 6 cut(s) 125, 337, 647, 770, 962, 1142
Fsp4HI GCNGC 4 cut(s) 1311, 1321, 1366, 1454
FspBI CTAG 1 cut(s) 441
GlaI GCGC 1 cut(s) 1314
GluI GCNGC 4 cut(s) 1311, 1321, 1366, 1454
GsaI CCCAGC 1 cut(s) 349
GsuI CTGGAG 1 cut(s) 143
HaeIII GGCC 2 cut(s) 415, 1393
HapII CCGG 3 cut(s) 112, 224, 1307
HhaI GCGC 1 cut(s) 1315
Hin1II CATG 7 cut(s) 40, 262, 448, 489, 1238, 1384, 1517
Hin6I GCGC 1 cut(s) 1313
HinP1I GCGC 1 cut(s) 1313
HinfI GANTC 7 cut(s) 281, 307, 407, 841, 861, 905, 926
HpaII CCGG 3 cut(s) 112, 224, 1307
HphI GGTGA 4 cut(s) 11, 246, 1264, 1279
Hpy166II GTNNAC 6 cut(s) 117, 711, 781, 816, 1346, 1482
Hpy188III TCNNGA 2 cut(s) 278, 1127
Hpy8I GTNNAC 6 cut(s) 117, 711, 781, 816, 1346, 1482
Hpy99I CGWCG 1 cut(s) 886
HpyAV CCTTC 9 cut(s) 22, 177, 210, 499, 670, 733, 1021, 1165, 1277
HpyCH4IV ACGT 4 cut(s) 713, 884, 1208, 1277
HpyCH4V TGCA 8 cut(s) 592, 711, 1040, 1058, 1102, 1206, 1340, 1498
HpyF10VI GCNNNNNNNGC 1 cut(s) 506
HpyF3I CTNAG 4 cut(s) 248, 501, 810, 1011
HpySE526I ACGT 4 cut(s) 713, 884, 1208, 1277
Hsp92II CATG 7 cut(s) 40, 262, 448, 489, 1238, 1384, 1517
HspAI GCGC 1 cut(s) 1313
KroI GCCGGC 1 cut(s) 223
KroNI GCCGGC 1 cut(s) 225
Ksp22I TGATCA 3 cut(s) 868, 1045, 1269
KspI CCGCGG 1 cut(s) 695
Kzo9I GATC 6 cut(s) 108, 749, 824, 868, 1045, 1269
LmnI GCTCC 5 cut(s) 296, 370, 577, 989, 1191
Lsp1109I GCAGC 4 cut(s) 1297, 1332, 1377, 1465
LweI GCATC 2 cut(s) 1067, 1256
MaeI CTAG 1 cut(s) 441
MaeII ACGT 4 cut(s) 713, 884, 1208, 1277
MaeIII GTNAC 1 cut(s) 153
MalI GATC 6 cut(s) 110, 751, 826, 870, 1047, 1271
MboI GATC 6 cut(s) 108, 749, 824, 868, 1045, 1269
MfeI CAATTG 2 cut(s) 646, 939
MhlI GDGCHC 2 cut(s) 574, 713
MlyI GAGTC 2 cut(s) 316, 914
MmeI TCCRAC 3 cut(s) 311, 424, 905
Mph1103I ATGCAT 1 cut(s) 1060
MroNI GCCGGC 1 cut(s) 223
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 3 cut(s) 357, 495, 564
MslI CAYNNNNRTG 1 cut(s) 1385
MspA1I CMGCKG 3 cut(s) 48, 694, 1323
MspI CCGG 3 cut(s) 112, 224, 1307
MunI CAATTG 2 cut(s) 646, 939
Mva1269I GAATGC 1 cut(s) 592
MvnI CGCG 2 cut(s) 694, 1315
MwoI GCNNNNNNNGC 1 cut(s) 506
NaeI GCCGGC 1 cut(s) 225
NcoI CCATGG 1 cut(s) 1380
NdeI CATATG 1 cut(s) 1054
NdeII GATC 6 cut(s) 108, 749, 824, 868, 1045, 1269
NgoMIV GCCGGC 1 cut(s) 223
NlaIII CATG 7 cut(s) 40, 262, 448, 489, 1238, 1384, 1517
NlaIV GGNNCC 1 cut(s) 372
NmuCI GTSAC 1 cut(s) 153
NsiI ATGCAT 1 cut(s) 1060
NspI RCATGY 1 cut(s) 1238
PceI AGGCCT 1 cut(s) 415
PciI ACATGT 1 cut(s) 1234
PcsI WCGNNNNNNNCGW 1 cut(s) 534
PctI GAATGC 1 cut(s) 592
PdiI GCCGGC 1 cut(s) 225
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 5 cut(s) 281, 407, 841, 861, 926
Pfl23II CGTACG 1 cut(s) 1278
PflFI GACNNNGTC 1 cut(s) 886
PkrI GCNGC 4 cut(s) 1312, 1322, 1367, 1455
PleI GAGTC 2 cut(s) 315, 913
PpsI GAGTC 2 cut(s) 315, 913
Ppu21I YACGTR 1 cut(s) 1278
PscI ACATGT 1 cut(s) 1234
PspFI CCCAGC 1 cut(s) 345
PspLI CGTACG 1 cut(s) 1278
PspN4I GGNNCC 1 cut(s) 372
PspPI GGNCC 1 cut(s) 1392
PsyI GACNNNGTC 1 cut(s) 886
RsaI GTAC 3 cut(s) 782, 1280, 1481
RsaNI GTAC 3 cut(s) 781, 1279, 1480
RseI CAYNNNNRTG 1 cut(s) 1385
SacII CCGCGG 1 cut(s) 695
SaqAI TTAA 3 cut(s) 357, 495, 564
SatI GCNGC 4 cut(s) 1311, 1321, 1366, 1454
Sau3AI GATC 6 cut(s) 108, 749, 824, 868, 1045, 1269
Sau96I GGNCC 1 cut(s) 1392
SchI GAGTC 2 cut(s) 316, 914
SduI GDGCHC 2 cut(s) 574, 713
SfaNI GCATC 2 cut(s) 1067, 1256
SfcI CTRYAG 1 cut(s) 917
Sfr303I CCGCGG 1 cut(s) 695
SgrBI CCGCGG 1 cut(s) 695
SmiMI CAYNNNNRTG 1 cut(s) 1385
SseBI AGGCCT 1 cut(s) 415
SsiI CCGC 9 cut(s) 48, 237, 509, 692, 694, 1068, 1265, 1323, 1505
SspMI CTAG 1 cut(s) 441
StuI AGGCCT 1 cut(s) 415
StyI CCWWGG 3 cut(s) 1187, 1380, 1395
TaiI ACGT 4 cut(s) 716, 887, 1211, 1280
TaqI TCGA 7 cut(s) 305, 318, 410, 528, 537, 839, 1119
TatI WGTACW 1 cut(s) 780
TfiI GAWTC 5 cut(s) 281, 407, 841, 861, 926
Tru1I TTAA 3 cut(s) 357, 495, 564
Tru9I TTAA 3 cut(s) 357, 495, 564
TscAI CASTG 3 cut(s) 459, 1230, 1471
TseFI GTSAC 1 cut(s) 153
TseI GCWGC 4 cut(s) 1310, 1320, 1365, 1453
Tsp45I GTSAC 1 cut(s) 153
TspDTI ATGAA 3 cut(s) 341, 573, 1014
TspGWI ACGGA 4 cut(s) 134, 865, 898, 1201
TspRI CASTG 3 cut(s) 459, 1230, 1471
Tth111I GACNNNGTC 1 cut(s) 886
VneI GTGCAC 1 cut(s) 709
XapI RAATTY 1 cut(s) 524
XceI RCATGY 1 cut(s) 1238
XmiI GTMKAC 2 cut(s) 815, 1345
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 1 cut(s) 441
Zsp2I ATGCAT 1 cut(s) 1060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.