Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0002634

Physical Location & Seq

Genomic Coordinates Reverse (-)

29722631 .. 29726549

Gene Structure 3919 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g37750.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1524 bp

ATGTCAGCTGAATTTCCGGTAAGAGCTCGCGGCCGCTTGGCCGTGCTCACGGCCCATCTCGCCGCCGCCACCATCGCCGAGCCCACGGAGCTCGAGCCGCAGTGCTTGTCGGCCGCGGTCCCACCTCCGGGCAATCTCTGCGGCGGTTTGACCATCGTTGACGAGAGGACCGGTAAGCGGTACCAGGTCCAAGTCTCCGAACAAGGAACCATCAAAGCCACTGATCTCAAAAAGATTACGACGGGGAAGAATGATAAGGGGCTGAAGCTGTATGATCCGGGTTATCTGAACACGGCGCCGGTTCGATCTTCGATTTGCTATATTGATGGAGATGAGGGGATTCTTAGGTACAGAGGCTACCCAATTGAGGAATTGGCTGAGAGTAGTACCTTTCTAGAAGTTGCATATCTCCTCATGTATGGGAATTTGCCTACTCAAAGCCAGTTAGCGGATTGGGAATTTGCTGTTTCTCAGCATTCAGCTGTACCACAGGGGATTTTGGATATTATACAAGCAATACCTCATGATGCACATCCAATGGGTGTACTTGTCAGCGCAATGAGTGCTCTTTCTGTTTTTCATCCTGATGCCAATCCTGCCCTCAGAGGGCAAGACCTTTATCAGTCGAAACAAGTCAGAGACAAACAAATTGCACGGATTCTTGGGAAGGCACCAACTATTGCAGCAGCAGCTTATTTGAGATTGGCAGGAAGGCCCGCAGTTCTACCATCTAACAACCTTTCTTATTCCGAGAACTTCTTATACATGCTAGACTCCTTGGGCAATAGAGCTTACAAACCAAATCCTCGACTTGCCCGAGTACTGGACATTCTCTTCATACTCCATGCCGAACATGAGATGAATTGCTCAACAGCTGCTGCTCGTCATCTGGCATCAAGTGGTGTGGATGTTTACACTGCCCTTGCTGGAGCTGTTGGAGCTCTATATGGTCCCCTTCATGGTGGAGCAAATGAGGCTGTGCTTAAGATGCTAAGTGAAATTGGATCTGTTGAGAACATTCCAGAGTTCATTGAGGGCGTGAAGAGCAGGAAGCGAAAGATGTCAGGTTTTGGGCATCGTGTATACAAGAACTATGATCCTAGAGCAAAGGTCATAAGAAAGCTGGCAGATGAAGTATTCTCCATTGTTGGAAGGGATCCACTAATTGAGGTAGCTGTTGCTTTGGAGAAAGCTGCACTATCTGATGAGTATTTTGTTAAGAGAAAGCTGTATCCAAATGTTGATTTCTACTCTGGGTTGATATATAGGGCAATGGGATTTCCAACGGAGTTCTTCCCAGTTTTGTTTGCAATCCCTCGAATGGCTGGTTACTTGTCACACTGGAGAGAATCCCTGGATGATCCTGATACGAAGATTATGAGGCCTGCACAGGTATATGTTGGGGCGTGGCTGCGGCACTATATGCCACTGAAAGAACGGATAGTAAGCAGTGATGGAGACAAACTGTCTCAAGTTTCTGTTTCAAACGCCTCTAAGCGTCGTTTGTCTGGATCTGGAGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004108 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005911 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009506 GO:0009514 GO:0009987 GO:0016042 GO:0016054 GO:0016740 GO:0016746 GO:0019395 GO:0019752 GO:0030054 GO:0030258 GO:0032787 GO:0034440 GO:0036440 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0046912 GO:0055044 GO:0055114 GO:0071704 GO:0072329 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00020 ko00630 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Citrate_synt

Protein Analysis

508

Amino Acids

55.61

Weight (kDa)

8.75

Isoelectric Point (pI)

38.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Citrate_synt	PF00285	94 - 463	1.8e-134	Citrate synthase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002634)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42790 AT3G58740 AT3G58750
fragaria_vesca	FvH4_5g27540 FvH4_6g37750
malus_domestica	MD09G1156400.v1.1 MD17G1142900.v1.1
prunus_persica	Prupe.3G169400_v2.0.a1
pyrus_communis	pycom09g07510 pycom17g13590 pycom17g13600
rosa_chinensis	RchiOBHm_Chr2g0143111 RchiOBHm_Chr2g0150811 RchiOBHm_Chr7g0233461
rosa_laevigata	RLG00000020508
rosa_multiflora	Rmu_co8164368.1_g000001 Rmu_co8192116.1_g000001 Rmu_co8272387.1_g000001 Rmu_sc0003819.1_g000005 Rmu_sc0004794.1_g000003 Rmu_sc0020410.1_g000001
rosa_roxburghii	Rroxscaffold_2G00096940 Rroxscaffold_3G00251810
rosa_rugosa	Rorug02G0103600 Rorug02G0179700 Rorug02G0422600
rosa_samantha	Rh2AG024100 Rh2AG482700 Rh2BG495300 Rh2CG469000 Rh2DG450300 Rh2DG506100 Rh4CG229100 Rh4DG032400 Rh4DG200800 Rh5AG029300 Rh5CG031600 Rh5DG497900 Rh6CG201800
rosa_wichuraiana	Rw2G039560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	180
AccB1I	GGYRCC	3 cut(s)	180, 295, 670
AccI	GTMKAC	1 cut(s)	1083
AccII	CGCG	2 cut(s)	30, 116
AclWI	GGATC	7 cut(s)	269, 1012, 1091, 1151, 1164, 1355, 1519
AcoI	YGGCCR	3 cut(s)	31, 39, 111
AcsI	RAATTY	3 cut(s)	11, 424, 458
AcuI	CTGAAG	1 cut(s)	284
AcyI	GRCGYC	1 cut(s)	296
AfaI	GTAC	6 cut(s)	182, 350, 388, 486, 546, 822
AfiI	CCNNNNNNNGG	9 cut(s)	127, 128, 177, 367, 448, 606, 823, 959, 1321
AflII	CTTAAG	1 cut(s)	983
AgeI	ACCGGT	1 cut(s)	170
AgsI	TTSAA	1 cut(s)	1485
AhdI	GACNNNNNGTC	1 cut(s)	1465
AjnI	CCWGG	2 cut(s)	183, 1353
Alw21I	GWGCWC	5 cut(s)	28, 48, 93, 568, 943
Alw26I	GTCTC	4 cut(s)	199, 633, 1452, 1473
AlwI	GGATC	7 cut(s)	269, 1012, 1091, 1151, 1164, 1355, 1519
AlwNI	CAGNNNCTG	1 cut(s)	878
Ama87I	CYCGRG	2 cut(s)	92, 816
AoxI	GGCC	6 cut(s)	31, 39, 51, 111, 713, 1382
ApeKI	GCWGC	7 cut(s)	683, 686, 689, 875, 878, 1193, 1411
ApoI	RAATTY	3 cut(s)	11, 424, 458
AsiGI	ACCGGT	1 cut(s)	170
Asp718I	GGTACC	1 cut(s)	180
AspLEI	GCGC	2 cut(s)	298, 557
AspS9I	GGNCC	6 cut(s)	52, 118, 168, 187, 714, 950
AsuC2I	CCSGG	2 cut(s)	129, 279
AvaI	CYCGRG	2 cut(s)	92, 816
AvaII	GGWCC	4 cut(s)	118, 168, 187, 950
BamHI	GGATCC	1 cut(s)	1156
BanI	GGYRCC	3 cut(s)	180, 295, 670
BanII	GRGCYC	4 cut(s)	28, 84, 93, 943
Bbv12I	GWGCWC	5 cut(s)	28, 48, 93, 568, 943
BbvI	GCAGC	7 cut(s)	695, 698, 701, 862, 865, 1180, 1398
BccI	CCATC	7 cut(s)	63, 80, 161, 218, 320, 736, 1448
BceAI	ACGGC	3 cut(s)	26, 66, 309
BciT130I	CCWGG	2 cut(s)	185, 1355
BciVI	GTATCC	1 cut(s)	1242
BcnI	CCSGG	2 cut(s)	129, 279
BcoDI	GTCTC	4 cut(s)	199, 633, 1452, 1473
BfaI	CTAG	3 cut(s)	395, 770, 1101
BfoI	RGCGCY	1 cut(s)	299
BfrI	CTTAAG	1 cut(s)	983
BfuI	GTATCC	1 cut(s)	1242
BmcAI	AGTACT	1 cut(s)	822
Bme1390I	CCNGG	4 cut(s)	129, 185, 279, 1355
Bme18I	GGWCC	4 cut(s)	118, 168, 187, 950
BmeRI	GACNNNNNGTC	1 cut(s)	1465
BmeT110I	CYCGRG	2 cut(s)	92, 816
BmgT120I	GGNCC	6 cut(s)	52, 118, 168, 187, 714, 950
BmiI	GGNNCC	7 cut(s)	120, 182, 208, 297, 672, 952, 1158
BmrFI	CCNGG	4 cut(s)	129, 185, 279, 1355
BmrI	ACTGGG	1 cut(s)	1292
BmsI	GCATC	5 cut(s)	517, 577, 902, 978, 1084
BmuI	ACTGGG	1 cut(s)	1292
BpmI	CTGGAG	2 cut(s)	948, 1363
BpuEI	CTTGAG	1 cut(s)	1455
BpuMI	CCSGG	2 cut(s)	129, 279
BsaBI	GATNNNNATC	2 cut(s)	531, 591
BsaHI	GRCGYC	1 cut(s)	296
BsaJI	CCNNGG	4 cut(s)	84, 114, 777, 1353
BsaWI	WCCGGW	2 cut(s)	16, 170
Bsc4I	CCNNNNNNNGG	9 cut(s)	127, 128, 177, 367, 448, 606, 823, 959, 1321
Bse118I	RCCGGY	2 cut(s)	170, 298
Bse1I	ACTGG	4 cut(s)	442, 828, 1298, 1346
Bse3DI	GCAATG	2 cut(s)	564, 1278
Bse8I	GATNNNNATC	2 cut(s)	531, 591
BseBI	CCWGG	2 cut(s)	185, 1355
BseDI	CCNNGG	4 cut(s)	84, 114, 777, 1353
BseGI	GGATG	4 cut(s)	532, 580, 913, 1363
BseJI	GATNNNNATC	2 cut(s)	531, 591
BseLI	CCNNNNNNNGG	9 cut(s)	127, 128, 177, 367, 448, 606, 823, 959, 1321
BseMI	GCAATG	2 cut(s)	564, 1278
BseMII	CTCAG	3 cut(s)	369, 485, 616
BseNI	ACTGG	4 cut(s)	442, 828, 1298, 1346
BseRI	GAGGAG	1 cut(s)	401
BseX3I	CGGCCG	2 cut(s)	31, 111
BseXI	GCAGC	7 cut(s)	695, 698, 701, 862, 865, 1180, 1398
BsgI	GTGCAG	2 cut(s)	1179, 1371
Bsh1236I	CGCG	2 cut(s)	30, 116
Bsh1285I	CGRYCG	2 cut(s)	34, 114
BshFI	GGCC	6 cut(s)	33, 41, 53, 113, 715, 1384
BshNI	GGYRCC	3 cut(s)	180, 295, 670
BshTI	ACCGGT	1 cut(s)	170
BsiEI	CGRYCG	2 cut(s)	34, 114
BsiHKAI	GWGCWC	5 cut(s)	28, 48, 93, 568, 943
BsiHKCI	CYCGRG	2 cut(s)	92, 816
BsiSI	CCGG	5 cut(s)	17, 128, 171, 278, 299
BslFI	GGGAC	2 cut(s)	104, 936
BslI	CCNNNNNNNGG	9 cut(s)	127, 128, 177, 367, 448, 606, 823, 959, 1321
BsmAI	GTCTC	4 cut(s)	199, 633, 1452, 1473
BsmFI	GGGAC	2 cut(s)	104, 936
BsmI	GAATGC	1 cut(s)	475
BsnI	GGCC	6 cut(s)	33, 41, 53, 113, 715, 1384
BsoBI	CYCGRG	2 cut(s)	92, 816
Bsp1286I	GDGCHC	6 cut(s)	28, 48, 84, 93, 568, 943
Bsp143I	GATC	8 cut(s)	223, 274, 305, 1004, 1096, 1156, 1360, 1511
BspANI	GGCC	6 cut(s)	33, 41, 53, 113, 715, 1384
BspCNI	CTCAG	3 cut(s)	370, 484, 615
BspFNI	CGCG	2 cut(s)	30, 116
BspHI	TCATGA	1 cut(s)	523
BspLI	GGNNCC	7 cut(s)	120, 182, 208, 297, 672, 952, 1158
BspPI	GGATC	7 cut(s)	269, 1012, 1091, 1151, 1164, 1355, 1519
BspQI	GCTCTTC	1 cut(s)	1037
BspT107I	GGYRCC	3 cut(s)	180, 295, 670
BspTI	CTTAAG	1 cut(s)	983
BsrDI	GCAATG	2 cut(s)	564, 1278
BsrFI	RCCGGY	2 cut(s)	170, 298
BsrI	ACTGG	4 cut(s)	442, 828, 1298, 1346
BssAI	RCCGGY	2 cut(s)	170, 298
BssECI	CCNNGG	4 cut(s)	84, 114, 777, 1353
BssMI	GATC	8 cut(s)	223, 274, 305, 1004, 1096, 1156, 1360, 1511
BssNAI	GTATAC	1 cut(s)	1084
BssNI	GRCGYC	1 cut(s)	296
BssT1I	CCWWGG	1 cut(s)	777
Bst1107I	GTATAC	1 cut(s)	1084
Bst2UI	CCWGG	2 cut(s)	185, 1355
Bst4CI	ACNGT	1 cut(s)	1467
Bst6I	CTCTTC	2 cut(s)	839, 1037
BstACI	GRCGYC	1 cut(s)	296
BstAFI	CTTAAG	1 cut(s)	983
BstAPI	GCANNNNNTGC	3 cut(s)	138, 563, 1423
BstC8I	GCNNGC	4 cut(s)	28, 717, 1125, 1386
BstDEI	CTNAG	6 cut(s)	344, 378, 471, 602, 992, 1494
BstDSI	CCRYGG	2 cut(s)	84, 114
BstF5I	GGATG	4 cut(s)	532, 580, 913, 1363
BstFNI	CGCG	2 cut(s)	30, 116
BstH2I	RGCGCY	1 cut(s)	299
BstHHI	GCGC	2 cut(s)	298, 557
BstKTI	GATC	8 cut(s)	226, 277, 308, 1007, 1099, 1159, 1363, 1514
BstMAI	GTCTC	4 cut(s)	199, 633, 1452, 1473
BstMBI	GATC	8 cut(s)	223, 274, 305, 1004, 1096, 1156, 1360, 1511
BstMCI	CGRYCG	2 cut(s)	34, 114
BstNI	CCWGG	2 cut(s)	185, 1355
BstNSI	RCATGY	1 cut(s)	769
BstSCI	CCNGG	4 cut(s)	127, 183, 277, 1353
BstUI	CGCG	2 cut(s)	30, 116
BstV1I	GCAGC	7 cut(s)	695, 698, 701, 862, 865, 1180, 1398
BstX2I	RGATCY	3 cut(s)	1004, 1156, 1511
BstYI	RGATCY	3 cut(s)	1004, 1156, 1511
BstZ17I	GTATAC	1 cut(s)	1084
BstZI	CGGCCG	2 cut(s)	31, 111
BsuI	GTATCC	1 cut(s)	1242
BsuRI	GGCC	6 cut(s)	33, 41, 53, 113, 715, 1384
BtgI	CCRYGG	2 cut(s)	84, 114
BtgZI	GCGATG	1 cut(s)	58
BtsCI	GGATG	4 cut(s)	532, 580, 913, 1363
BtsI	GCAGTG	3 cut(s)	107, 915, 1456
BtsIMutI	CAGTG	6 cut(s)	107, 219, 915, 1339, 1427, 1456
Cac8I	GCNNGC	4 cut(s)	28, 717, 1125, 1386
CaiI	CAGNNNCTG	1 cut(s)	878
CciI	TCATGA	1 cut(s)	523
CciNI	GCGGCCGC	1 cut(s)	31
CfoI	GCGC	2 cut(s)	298, 557
Cfr10I	RCCGGY	2 cut(s)	170, 298
Cfr13I	GGNCC	6 cut(s)	52, 118, 168, 187, 714, 950
Cfr42I	CCGCGG	1 cut(s)	117
CseI	GACGC	1 cut(s)	1487
CsiI	ACCWGGT	1 cut(s)	183
Csp6I	GTAC	6 cut(s)	181, 349, 387, 485, 545, 821
CspAI	ACCGGT	1 cut(s)	170
CspCI	CAANNNNNGTGG	2 cut(s)	885, 920
CviAII	CATG	6 cut(s)	415, 524, 766, 845, 854, 959
CviQI	GTAC	6 cut(s)	181, 349, 387, 485, 545, 821
DdeI	CTNAG	6 cut(s)	344, 378, 471, 602, 992, 1494
DinI	GGCGCC	1 cut(s)	297
DpnI	GATC	8 cut(s)	225, 276, 307, 1006, 1098, 1158, 1362, 1513
DpnII	GATC	8 cut(s)	223, 274, 305, 1004, 1096, 1156, 1360, 1511
DriI	GACNNNNNGTC	1 cut(s)	1465
EaeI	YGGCCR	3 cut(s)	31, 39, 111
EagI	CGGCCG	2 cut(s)	31, 111
Eam1104I	CTCTTC	2 cut(s)	839, 1037
Eam1105I	GACNNNNNGTC	1 cut(s)	1465
EarI	CTCTTC	2 cut(s)	839, 1037
Ecl136II	GAGCTC	3 cut(s)	26, 91, 941
EclXI	CGGCCG	2 cut(s)	31, 111
Eco130I	CCWWGG	1 cut(s)	777
Eco147I	AGGCCT	1 cut(s)	1384
Eco24I	GRGCYC	4 cut(s)	28, 84, 93, 943
Eco47I	GGWCC	4 cut(s)	118, 168, 187, 950
Eco52I	CGGCCG	2 cut(s)	31, 111
Eco53kI	GAGCTC	3 cut(s)	26, 91, 941
Eco57I	CTGAAG	1 cut(s)	284
Eco88I	CYCGRG	2 cut(s)	92, 816
EcoICRI	GAGCTC	3 cut(s)	26, 91, 941
EcoRII	CCWGG	2 cut(s)	183, 1353
EcoT14I	CCWWGG	1 cut(s)	777
EcoT38I	GRGCYC	4 cut(s)	28, 84, 93, 943
EgeI	GGCGCC	1 cut(s)	297
EheI	GGCGCC	1 cut(s)	297
ErhI	CCWWGG	1 cut(s)	777
FaeI	CATG	6 cut(s)	418, 527, 769, 848, 857, 962
FaqI	GGGAC	2 cut(s)	104, 936
FatI	CATG	6 cut(s)	414, 523, 765, 844, 853, 958
FauI	CCCGC	1 cut(s)	724
FblI	GTMKAC	1 cut(s)	1083
FokI	GGATG	4 cut(s)	519, 567, 920, 1370
FriOI	GRGCYC	4 cut(s)	28, 84, 93, 943
FspBI	CTAG	3 cut(s)	395, 770, 1101
GlaI	GCGC	2 cut(s)	297, 556
GsuI	CTGGAG	2 cut(s)	948, 1363
HaeII	RGCGCY	1 cut(s)	299
HaeIII	GGCC	6 cut(s)	33, 41, 53, 113, 715, 1384
HapII	CCGG	5 cut(s)	17, 128, 171, 278, 299
HgaI	GACGC	1 cut(s)	1487
HhaI	GCGC	2 cut(s)	298, 557
Hin1I	GRCGYC	1 cut(s)	296
Hin1II	CATG	6 cut(s)	418, 527, 769, 848, 857, 962
Hin6I	GCGC	2 cut(s)	296, 555
HinP1I	GCGC	2 cut(s)	296, 555
HincII	GTYRAC	1 cut(s)	160
HindII	GTYRAC	1 cut(s)	160
HinfI	GANTC	4 cut(s)	340, 658, 773, 1349
HpaII	CCGG	5 cut(s)	17, 128, 171, 278, 299
Hpy166II	GTNNAC	4 cut(s)	160, 545, 913, 1084
Hpy188I	TCNGA	6 cut(s)	199, 288, 605, 638, 751, 1204
Hpy188III	TCNNGA	7 cut(s)	395, 524, 584, 1022, 1364, 1509, 1515
Hpy8I	GTNNAC	4 cut(s)	160, 545, 913, 1084
Hpy99I	CGWCG	2 cut(s)	244, 1503
HpyAV	CCTTC	4 cut(s)	661, 705, 965, 1146
HpyCH4III	ACNGT	1 cut(s)	1467
HpyCH4V	TGCA	7 cut(s)	404, 530, 653, 683, 1196, 1310, 1388
HpyF3I	CTNAG	6 cut(s)	344, 378, 471, 602, 992, 1494
Hsp92I	GRCGYC	1 cut(s)	296
Hsp92II	CATG	6 cut(s)	418, 527, 769, 848, 857, 962
HspAI	GCGC	2 cut(s)	296, 555
KasI	GGCGCC	1 cut(s)	295
KpnI	GGTACC	1 cut(s)	184
KspI	CCGCGG	1 cut(s)	117
Kzo9I	GATC	8 cut(s)	223, 274, 305, 1004, 1096, 1156, 1360, 1511
LguI	GCTCTTC	1 cut(s)	1037
LmnI	GCTCC	4 cut(s)	88, 929, 938, 965
Lsp1109I	GCAGC	7 cut(s)	695, 698, 701, 862, 865, 1180, 1398
LweI	GCATC	5 cut(s)	517, 577, 902, 978, 1084
MabI	ACCWGGT	1 cut(s)	183
MaeI	CTAG	3 cut(s)	395, 770, 1101
MaeIII	GTNAC	2 cut(s)	1328, 1335
MalI	GATC	8 cut(s)	225, 276, 307, 1006, 1098, 1158, 1362, 1513
MboI	GATC	8 cut(s)	223, 274, 305, 1004, 1096, 1156, 1360, 1511
MboII	GAAGA	6 cut(s)	259, 300, 826, 1054, 1285, 1384
MfeI	CAATTG	1 cut(s)	363
MflI	RGATCY	3 cut(s)	1004, 1156, 1511
MhlI	GDGCHC	6 cut(s)	28, 48, 84, 93, 568, 943
MluCI	AATT	9 cut(s)	11, 363, 371, 424, 458, 648, 862, 999, 1164
Mly113I	GGCGCC	1 cut(s)	296
MlyI	GAGTC	1 cut(s)	767
MmeI	TCCRAC	3 cut(s)	916, 1129, 1307
MseI	TTAA	2 cut(s)	984, 1218
MslI	CAYNNNNRTG	1 cut(s)	585
MspA1I	CMGCKG	4 cut(s)	8, 116, 482, 875
MspCI	CTTAAG	1 cut(s)	983
MspI	CCGG	5 cut(s)	17, 128, 171, 278, 299
MspR9I	CCNGG	4 cut(s)	129, 185, 279, 1355
MunI	CAATTG	1 cut(s)	363
Mva1269I	GAATGC	1 cut(s)	475
MvaI	CCWGG	2 cut(s)	185, 1355
MvnI	CGCG	2 cut(s)	30, 116
NarI	GGCGCC	1 cut(s)	296
NciI	CCSGG	2 cut(s)	129, 279
NdeII	GATC	8 cut(s)	223, 274, 305, 1004, 1096, 1156, 1360, 1511
NlaIII	CATG	6 cut(s)	418, 527, 769, 848, 857, 962
NlaIV	GGNNCC	7 cut(s)	120, 182, 208, 297, 672, 952, 1158
NmeAIII	GCCGAG	1 cut(s)	103
NmuCI	GTSAC	1 cut(s)	1335
NotI	GCGGCCGC	1 cut(s)	31
NspI	RCATGY	1 cut(s)	769
PaeR7I	CTCGAG	1 cut(s)	92
PagI	TCATGA	1 cut(s)	523
PceI	AGGCCT	1 cut(s)	1384
PciSI	GCTCTTC	1 cut(s)	1037
PcsI	WCGNNNNNNNCGW	1 cut(s)	814
PctI	GAATGC	1 cut(s)	475
PfeI	GAWTC	3 cut(s)	340, 658, 1349
PinAI	ACCGGT	1 cut(s)	170
PleI	GAGTC	1 cut(s)	767
PluTI	GGCGCC	1 cut(s)	299
PpsI	GAGTC	1 cut(s)	767
Psp124BI	GAGCTC	3 cut(s)	28, 93, 943
Psp6I	CCWGG	2 cut(s)	183, 1353
PspGI	CCWGG	2 cut(s)	183, 1353
PspN4I	GGNNCC	7 cut(s)	120, 182, 208, 297, 672, 952, 1158
PspPI	GGNCC	6 cut(s)	52, 118, 168, 187, 714, 950
PspXI	VCTCGAGB	1 cut(s)	92
PsrI	GAACNNNNNNTAC	2 cut(s)	746, 778
PstNI	CAGNNNCTG	1 cut(s)	878
PsuI	RGATCY	3 cut(s)	1004, 1156, 1511
PvuII	CAGCTG	3 cut(s)	8, 482, 875
RsaI	GTAC	6 cut(s)	182, 350, 388, 486, 546, 822
RsaNI	GTAC	6 cut(s)	181, 349, 387, 485, 545, 821
RseI	CAYNNNNRTG	1 cut(s)	585
SacI	GAGCTC	3 cut(s)	28, 93, 943
SacII	CCGCGG	1 cut(s)	117
SapI	GCTCTTC	1 cut(s)	1037
SaqAI	TTAA	2 cut(s)	984, 1218
Sau3AI	GATC	8 cut(s)	223, 274, 305, 1004, 1096, 1156, 1360, 1511
Sau96I	GGNCC	6 cut(s)	52, 118, 168, 187, 714, 950
ScaI	AGTACT	1 cut(s)	822
SchI	GAGTC	1 cut(s)	767
ScrFI	CCNGG	4 cut(s)	129, 185, 279, 1355
SduI	GDGCHC	6 cut(s)	28, 48, 84, 93, 568, 943
SexAI	ACCWGGT	1 cut(s)	183
SfaNI	GCATC	5 cut(s)	517, 577, 902, 978, 1084
SfoI	GGCGCC	1 cut(s)	297
Sfr274I	CTCGAG	1 cut(s)	92
Sfr303I	CCGCGG	1 cut(s)	117
SgrBI	CCGCGG	1 cut(s)	117
SinI	GGWCC	4 cut(s)	118, 168, 187, 950
SlaI	CTCGAG	1 cut(s)	92
SmiMI	CAYNNNNRTG	1 cut(s)	585
SmlI	CTYRAG	3 cut(s)	92, 983, 1470
SmoI	CTYRAG	3 cut(s)	92, 983, 1470
Sse9I	AATT	9 cut(s)	11, 363, 371, 424, 458, 648, 862, 999, 1164
SseBI	AGGCCT	1 cut(s)	1384
SspDI	GGCGCC	1 cut(s)	295
SspMI	CTAG	3 cut(s)	395, 770, 1101
SstI	GAGCTC	3 cut(s)	28, 93, 943
StuI	AGGCCT	1 cut(s)	1384
StyD4I	CCNGG	4 cut(s)	127, 183, 277, 1353
StyI	CCWWGG	1 cut(s)	777
TaaI	ACNGT	1 cut(s)	1467
TaqI	TCGA	6 cut(s)	93, 304, 311, 626, 808, 1318
TasI	AATT	9 cut(s)	11, 363, 371, 424, 458, 648, 862, 999, 1164
TatI	WGTACW	2 cut(s)	544, 820
TauI	GCSGC	8 cut(s)	33, 36, 65, 68, 100, 116, 144, 1417
TfiI	GAWTC	3 cut(s)	340, 658, 1349
Tru1I	TTAA	2 cut(s)	984, 1218
Tru9I	TTAA	2 cut(s)	984, 1218
TscAI	CASTG	6 cut(s)	107, 226, 922, 1346, 1434, 1456
TseFI	GTSAC	1 cut(s)	1335
TseI	GCWGC	7 cut(s)	683, 686, 689, 875, 878, 1193, 1411
Tsp45I	GTSAC	1 cut(s)	1335
TspDTI	ATGAA	6 cut(s)	569, 826, 875, 947, 1018, 1146
TspGWI	ACGGA	4 cut(s)	101, 670, 1301, 1453
TspRI	CASTG	6 cut(s)	107, 226, 922, 1346, 1434, 1456
Vha464I	CTTAAG	1 cut(s)	983
VpaK11BI	GGWCC	4 cut(s)	118, 168, 187, 950
XapI	RAATTY	3 cut(s)	11, 424, 458
XbaI	TCTAGA	1 cut(s)	394
XceI	RCATGY	1 cut(s)	769
XhoI	CTCGAG	1 cut(s)	92
XmiI	GTMKAC	1 cut(s)	1083
XspI	CTAG	3 cut(s)	395, 770, 1101
ZrmI	AGTACT	1 cut(s)	822

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.