Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0002634

Physical Location & Seq

Genomic Coordinates Forward (+)

12887140 .. 12891488

Gene Structure 4349 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1142900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1539 bp

ATGTCGAGGGTGACGGAATTTCCGGTGACAGCTCGCGGTCGCTTGGCCGTGCTCTCAGCCCACCTGGCAGCTGCGACCATCGAGCCCTATGAGCTCGGACCCGCTCTCGAGCCCCACTGCGCGTCCGCTCACGTCCCACCGCCCGGGAACCTTCGGGGACCTTTGACCATCGTCGACGAGAGGACCGGTAAGAGGTATCAGGTCCAGGTCTCTGAGGAAGGAACCGTCAAAGCCACTGATCTCAAGAAGATCACAACAGGCAAAAATGACAAGGGGCTGAAGGTGTATGATCCAGGCTATCTTAATACCGCCCCTGTTCGATCCTCGATTTGTTATATCGATGGCGACGAGGGGATTCTTAGATACAGAGGCTACCCAATAGAGGAATTGGCTGAGAGTAGTACCTTTTTGGAAGTGGCCTATCTCTTGATGTATGGGAATTTGCCTTCTGAAAGTCAATATGTGGATTGGGAATTTGCCGTTTCTCAGCATTCAGCTGTACCACAGGGAATTTTGGACATTATACAGGCAATACCTCATGATGCACATCCAATGGGCGTACTTGTCAGTGCCATGAGTGCGCTTTCTGTTTTCCATCCTGATGCTAATCCTGCTCTTCGTGGGCAAGAAATTTACAAATCAAAACAAGTGAGAGACAAGCAAATAGCACGCATCCTTGGGAAGGCACCAACCATTGCAGCTGCAGCTTATTTGCGATTGGCTGGAAGACCTGCAGTTCTCCCTGCTAACAACCTTTCGTATTCGGAGAACTTCTTATACATGCTAGATTCCTTAGGCAATAGATCTTACAAACCCAATCCTCGACTGGCTCGAGTGCTAGATGTTCTCTTCATATTGCATGCAGAACATGAGATGAATTGCTCCACAGCTGCTGCCCGGCATCTGGCATCAAGCGGTGTCGATGTTTACACTGCTCTTGCTGGAGCTACAGGAGCTCTCTATGGCCCTCTTCATGGTGGAGCAAATGAGGCTGTGCTTAAGATGCTAAATGAGATTGGAACTGTTGAGAACATTCCAGATTTCATAGAGGGTGTGAAGAACAGGAAGCGAAAGATGTCAGGTTTTGGGCATCGTGTGTACAAAAACTATGATCCTAGAGCAAAGGTCATAAGGAAACTGGCAGAGGAAGTATTTTCCATTGTTGGAAGGGATCCTCTGATTGAGGTAGCTGTTGCTTTGGAGAAGGCTGCACTTTCTGACGAGTATTTTGTCAAGAGAAAGCTCTATCCAAATGTTGATTTCTACTCCGGTTTAATTTATAGGGCAATGGGATTTCCAACTGAGTTCTTCCCGGTTTTGTTCGCGATCCCTCGAATGGCTGGGTGGTTGGCACACTGGCGCGAATCCCTTGATGATCCTGATACTAAGATCATGAGACCCCAACAGGTGTATACTGGAAACTGGCTGCGCCACTATATCCCACCAAGAGAACGCATAGTAACGAGTGATGCAGACAAGCTCTCTCAGGTTTCTGTATCAAATGCCTCTAGGCGTCGGCTGGCTGGATCTGGAGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004108 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005911 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009506 GO:0009514 GO:0009987 GO:0016042 GO:0016054 GO:0016740 GO:0016746 GO:0019395 GO:0019752 GO:0030054 GO:0030258 GO:0032787 GO:0034440 GO:0036440 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0046912 GO:0055044 GO:0055114 GO:0071704 GO:0072329 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00020 ko00630 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Citrate_synt

Protein Analysis

513

Amino Acids

56.53

Weight (kDa)

8.79

Isoelectric Point (pI)

37.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Citrate_synt	PF00285	99 - 468	2.7e-135	Citrate synthase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002634)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42790 AT3G58740 AT3G58750
fragaria_vesca	FvH4_5g27540 FvH4_6g37750
malus_domestica	MD09G1156400.v1.1 MD17G1142900.v1.1
prunus_persica	Prupe.3G169400_v2.0.a1
pyrus_communis	pycom09g07510 pycom17g13590 pycom17g13600
rosa_chinensis	RchiOBHm_Chr2g0143111 RchiOBHm_Chr2g0150811 RchiOBHm_Chr7g0233461
rosa_laevigata	RLG00000020508
rosa_multiflora	Rmu_co8164368.1_g000001 Rmu_co8192116.1_g000001 Rmu_co8272387.1_g000001 Rmu_sc0003819.1_g000005 Rmu_sc0004794.1_g000003 Rmu_sc0020410.1_g000001
rosa_roxburghii	Rroxscaffold_2G00096940 Rroxscaffold_3G00251810
rosa_rugosa	Rorug02G0103600 Rorug02G0179700 Rorug02G0422600
rosa_samantha	Rh2AG024100 Rh2AG482700 Rh2BG495300 Rh2CG469000 Rh2DG450300 Rh2DG506100 Rh4CG229100 Rh4DG032400 Rh4DG200800 Rh5AG029300 Rh5CG031600 Rh5DG497900 Rh6CG201800
rosa_wichuraiana	Rw2G039560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	739
AccB1I	GGYRCC	1 cut(s)	685
AccBSI	CCGCTC	2 cut(s)	104, 128
AccI	GTMKAC	2 cut(s)	174, 1412
AccII	CGCG	4 cut(s)	36, 122, 1325, 1362
AciI	CCGC	6 cut(s)	36, 102, 126, 140, 309, 915
AclWI	GGATC	8 cut(s)	284, 315, 1106, 1166, 1179, 1321, 1370, 1534
AcoI	YGGCCR	1 cut(s)	45
AcsI	RAATTY	5 cut(s)	17, 439, 473, 510, 630
AcuI	CTGAAG	1 cut(s)	299
AcyI	GRCGYC	1 cut(s)	1513
AfaI	GTAC	4 cut(s)	403, 501, 561, 1100
AfiI	CCNNNNNNNGG	7 cut(s)	143, 192, 382, 682, 904, 974, 1336
AflII	CTTAAG	1 cut(s)	998
AgeI	ACCGGT	1 cut(s)	185
AjiI	CACGTC	1 cut(s)	133
AjnI	CCWGG	3 cut(s)	63, 204, 292
Alw21I	GWGCWC	3 cut(s)	54, 96, 958
Alw26I	GTCTC	3 cut(s)	214, 648, 1390
AlwI	GGATC	8 cut(s)	284, 315, 1106, 1166, 1179, 1321, 1370, 1534
AlwNI	CAGNNNCTG	1 cut(s)	893
Ama87I	CYCGRG	3 cut(s)	107, 143, 831
AoxI	GGCC	3 cut(s)	45, 417, 964
ApeKI	GCWGC	9 cut(s)	68, 71, 698, 701, 704, 890, 893, 1208, 1426
ApoI	RAATTY	5 cut(s)	17, 439, 473, 510, 630
ArsI	GACNNNNNNTTYG	2 cut(s)	1211, 1243
AsiGI	ACCGGT	1 cut(s)	185
AspLEI	GCGC	4 cut(s)	122, 583, 1362, 1431
AspS9I	GGNCC	5 cut(s)	98, 158, 183, 202, 965
AsuC2I	CCSGG	4 cut(s)	144, 145, 898, 1313
AsuHPI	GGTGA	2 cut(s)	22, 37
AvaI	CYCGRG	3 cut(s)	107, 143, 831
AvaII	GGWCC	4 cut(s)	98, 158, 183, 202
AxyI	CCTNAGG	1 cut(s)	793
BamHI	GGATCC	1 cut(s)	1171
BanI	GGYRCC	1 cut(s)	685
BanII	GRGCYC	4 cut(s)	87, 96, 114, 958
BbsI	GAAGAC	1 cut(s)	733
Bbv12I	GWGCWC	3 cut(s)	54, 96, 958
BbvI	GCAGC	9 cut(s)	58, 80, 688, 710, 716, 877, 880, 1195, 1413
BccI	CCATC	4 cut(s)	86, 176, 335, 603
BceAI	ACGGC	2 cut(s)	32, 464
BciT130I	CCWGG	3 cut(s)	65, 206, 294
BcnI	CCSGG	4 cut(s)	144, 145, 898, 1313
BcoDI	GTCTC	3 cut(s)	214, 648, 1390
BfaI	CTAG	4 cut(s)	785, 839, 1116, 1509
BfmI	CTRYAG	3 cut(s)	702, 732, 948
BfrI	CTTAAG	1 cut(s)	998
BfuAI	ACCTGC	1 cut(s)	739
BglI	GCCNNNNNGGC	1 cut(s)	65
BglII	AGATCT	1 cut(s)	803
BisI	GCNGC	9 cut(s)	69, 72, 699, 702, 705, 891, 894, 1209, 1427
BlsI	GCNGC	9 cut(s)	70, 73, 700, 703, 706, 892, 895, 1210, 1428
Bme1390I	CCNGG	7 cut(s)	65, 144, 145, 206, 294, 898, 1313
Bme18I	GGWCC	4 cut(s)	98, 158, 183, 202
BmeT110I	CYCGRG	3 cut(s)	107, 143, 831
BmgBI	CACGTC	1 cut(s)	133
BmgT120I	GGNCC	5 cut(s)	98, 158, 183, 202, 965
BmiI	GGNNCC	6 cut(s)	100, 149, 159, 223, 687, 1173
BmrFI	CCNGG	7 cut(s)	65, 144, 145, 206, 294, 898, 1313
BmsI	GCATC	8 cut(s)	532, 592, 681, 910, 917, 993, 1099, 1459
BoxI	GACNNNNGTC	1 cut(s)	170
BpiI	GAAGAC	1 cut(s)	733
BpmI	CTGGAG	1 cut(s)	963
BpuEI	CTTGAG	1 cut(s)	227
BpuMI	CCSGG	4 cut(s)	144, 145, 898, 1313
Bsa29I	ATCGAT	1 cut(s)	339
BsaBI	GATNNNNATC	2 cut(s)	546, 606
BsaHI	GRCGYC	1 cut(s)	1513
BsaI	GGTCTC	2 cut(s)	214, 1390
BsaJI	CCNNGG	2 cut(s)	143, 676
BsaWI	WCCGGW	3 cut(s)	22, 185, 1268
Bsc4I	CCNNNNNNNGG	7 cut(s)	143, 192, 382, 682, 904, 974, 1336
Bse118I	RCCGGY	1 cut(s)	185
Bse1I	ACTGG	5 cut(s)	831, 1143, 1361, 1420, 1427
Bse21I	CCTNAGG	1 cut(s)	793
Bse3DI	GCAATG	2 cut(s)	693, 1293
Bse8I	GATNNNNATC	2 cut(s)	546, 606
BseBI	CCWGG	3 cut(s)	65, 206, 294
BseCI	ATCGAT	1 cut(s)	339
BseDI	CCNNGG	2 cut(s)	143, 676
BseGI	GGATG	3 cut(s)	547, 595, 672
BseJI	GATNNNNATC	2 cut(s)	546, 606
BseLI	CCNNNNNNNGG	7 cut(s)	143, 192, 382, 682, 904, 974, 1336
BseMI	GCAATG	2 cut(s)	693, 1293
BseMII	CTCAG	6 cut(s)	69, 204, 384, 500, 1293, 1499
BseNI	ACTGG	5 cut(s)	831, 1143, 1361, 1420, 1427
BseXI	GCAGC	9 cut(s)	58, 80, 688, 710, 716, 877, 880, 1195, 1413
BseYI	CCCAGC	1 cut(s)	1340
BsgI	GTGCAG	1 cut(s)	1194
Bsh1236I	CGCG	4 cut(s)	36, 122, 1325, 1362
Bsh1285I	CGRYCG	1 cut(s)	40
BshFI	GGCC	3 cut(s)	47, 419, 966
BshNI	GGYRCC	1 cut(s)	685
BshTI	ACCGGT	1 cut(s)	185
BshVI	ATCGAT	1 cut(s)	339
BsiEI	CGRYCG	1 cut(s)	40
BsiHKAI	GWGCWC	3 cut(s)	54, 96, 958
BsiHKCI	CYCGRG	3 cut(s)	107, 143, 831
BsiSI	CCGG	6 cut(s)	23, 144, 186, 898, 1269, 1313
BslFI	GGGAC	2 cut(s)	119, 171
BslI	CCNNNNNNNGG	7 cut(s)	143, 192, 382, 682, 904, 974, 1336
BsmAI	GTCTC	3 cut(s)	214, 648, 1390
BsmFI	GGGAC	2 cut(s)	119, 171
BsmI	GAATGC	1 cut(s)	490
BsnI	GGCC	3 cut(s)	47, 419, 966
Bso31I	GGTCTC	2 cut(s)	214, 1390
BsoBI	CYCGRG	3 cut(s)	107, 143, 831
Bsp1286I	GDGCHC	5 cut(s)	54, 87, 96, 114, 958
Bsp1407I	TGTACA	1 cut(s)	1098
Bsp68I	TCGCGA	1 cut(s)	1325
BspACI	CCGC	6 cut(s)	36, 102, 126, 140, 309, 915
BspANI	GGCC	3 cut(s)	47, 419, 966
BspCNI	CTCAG	6 cut(s)	68, 205, 385, 499, 1294, 1498
BspDI	ATCGAT	1 cut(s)	339
BspFNI	CGCG	4 cut(s)	36, 122, 1325, 1362
BspHI	TCATGA	2 cut(s)	538, 1392
BspLI	GGNNCC	6 cut(s)	100, 149, 159, 223, 687, 1173
BspMAI	CTGCAG	2 cut(s)	706, 736
BspMI	ACCTGC	1 cut(s)	739
BspPI	GGATC	8 cut(s)	284, 315, 1106, 1166, 1179, 1321, 1370, 1534
BspQI	GCTCTTC	1 cut(s)	621
BspT107I	GGYRCC	1 cut(s)	685
BspTI	CTTAAG	1 cut(s)	998
BspTNI	GGTCTC	2 cut(s)	214, 1390
BsrBI	CCGCTC	2 cut(s)	104, 128
BsrDI	GCAATG	2 cut(s)	693, 1293
BsrFI	RCCGGY	1 cut(s)	185
BsrGI	TGTACA	1 cut(s)	1098
BsrI	ACTGG	5 cut(s)	831, 1143, 1361, 1420, 1427
BssAI	RCCGGY	1 cut(s)	185
BssECI	CCNNGG	2 cut(s)	143, 676
BssNAI	GTATAC	1 cut(s)	1413
BssNI	GRCGYC	1 cut(s)	1513
BssT1I	CCWWGG	1 cut(s)	676
Bst1107I	GTATAC	1 cut(s)	1413
Bst2UI	CCWGG	3 cut(s)	65, 206, 294
Bst4CI	ACNGT	2 cut(s)	226, 1024
Bst6I	CTCTTC	3 cut(s)	621, 854, 975
BstACI	GRCGYC	1 cut(s)	1513
BstAFI	CTTAAG	1 cut(s)	998
BstAUI	TGTACA	1 cut(s)	1098
BstC8I	GCNNGC	4 cut(s)	34, 670, 861, 1521
BstDEI	CTNAG	9 cut(s)	55, 213, 359, 393, 486, 793, 1302, 1386, 1485
BstENI	CCTNNNNNAGG	1 cut(s)	680
BstF5I	GGATG	3 cut(s)	547, 595, 672
BstFNI	CGCG	4 cut(s)	36, 122, 1325, 1362
BstHHI	GCGC	4 cut(s)	122, 583, 1362, 1431
BstMAI	GTCTC	3 cut(s)	214, 648, 1390
BstMCI	CGRYCG	1 cut(s)	40
BstMWI	GCNNNNNNNGC	8 cut(s)	65, 91, 578, 611, 704, 953, 989, 1003
BstNI	CCWGG	3 cut(s)	65, 206, 294
BstNSI	RCATGY	2 cut(s)	784, 863
BstPAI	GACNNNNGTC	1 cut(s)	170
BstSCI	CCNGG	7 cut(s)	63, 142, 143, 204, 292, 896, 1311
BstSFI	CTRYAG	3 cut(s)	702, 732, 948
BstUI	CGCG	4 cut(s)	36, 122, 1325, 1362
BstV1I	GCAGC	9 cut(s)	58, 80, 688, 710, 716, 877, 880, 1195, 1413
BstV2I	GAAGAC	1 cut(s)	733
BstX2I	RGATCY	3 cut(s)	803, 1171, 1526
BstYI	RGATCY	3 cut(s)	803, 1171, 1526
BstZ17I	GTATAC	1 cut(s)	1413
Bsu15I	ATCGAT	1 cut(s)	339
Bsu36I	CCTNAGG	1 cut(s)	793
BsuRI	GGCC	3 cut(s)	47, 419, 966
BsuTUI	ATCGAT	1 cut(s)	339
BtrI	CACGTC	1 cut(s)	133
BtsCI	GGATG	3 cut(s)	547, 595, 672
BtsI	GCAGTG	2 cut(s)	115, 930
BtsIMutI	CAGTG	5 cut(s)	115, 234, 574, 930, 1354
BtuMI	TCGCGA	1 cut(s)	1325
BveI	ACCTGC	1 cut(s)	739
Cac8I	GCNNGC	4 cut(s)	34, 670, 861, 1521
CaiI	CAGNNNCTG	1 cut(s)	893
CciI	TCATGA	2 cut(s)	538, 1392
CfoI	GCGC	4 cut(s)	122, 583, 1362, 1431
Cfr10I	RCCGGY	1 cut(s)	185
Cfr13I	GGNCC	5 cut(s)	98, 158, 183, 202, 965
Cfr9I	CCCGGG	1 cut(s)	143
ClaI	ATCGAT	1 cut(s)	339
CseI	GACGC	2 cut(s)	111, 1502
Csp6I	GTAC	4 cut(s)	402, 500, 560, 1099
CspAI	ACCGGT	1 cut(s)	185
CviAII	CATG	7 cut(s)	539, 574, 781, 860, 869, 974, 1393
CviQI	GTAC	4 cut(s)	402, 500, 560, 1099
DdeI	CTNAG	9 cut(s)	55, 213, 359, 393, 486, 793, 1302, 1386, 1485
EaeI	YGGCCR	1 cut(s)	45
Eam1104I	CTCTTC	3 cut(s)	621, 854, 975
EarI	CTCTTC	3 cut(s)	621, 854, 975
Ecl136II	GAGCTC	2 cut(s)	94, 956
Eco130I	CCWWGG	1 cut(s)	676
Eco24I	GRGCYC	4 cut(s)	87, 96, 114, 958
Eco31I	GGTCTC	2 cut(s)	214, 1390
Eco47I	GGWCC	4 cut(s)	98, 158, 183, 202
Eco53kI	GAGCTC	2 cut(s)	94, 956
Eco57I	CTGAAG	1 cut(s)	299
Eco81I	CCTNAGG	1 cut(s)	793
Eco88I	CYCGRG	3 cut(s)	107, 143, 831
EcoICRI	GAGCTC	2 cut(s)	94, 956
EcoNI	CCTNNNNNAGG	1 cut(s)	680
EcoO109I	RGGNCCY	1 cut(s)	158
EcoRII	CCWGG	3 cut(s)	63, 204, 292
EcoT14I	CCWWGG	1 cut(s)	676
EcoT38I	GRGCYC	4 cut(s)	87, 96, 114, 958
ErhI	CCWWGG	1 cut(s)	676
FaeI	CATG	7 cut(s)	542, 577, 784, 863, 872, 977, 1396
FaqI	GGGAC	2 cut(s)	119, 171
FatI	CATG	7 cut(s)	538, 573, 780, 859, 868, 973, 1392
FauI	CCCGC	1 cut(s)	109
FblI	GTMKAC	2 cut(s)	174, 1412
Fnu4HI	GCNGC	9 cut(s)	69, 72, 699, 702, 705, 891, 894, 1209, 1427
FokI	GGATG	3 cut(s)	534, 582, 659
FriOI	GRGCYC	4 cut(s)	87, 96, 114, 958
Fsp4HI	GCNGC	9 cut(s)	69, 72, 699, 702, 705, 891, 894, 1209, 1427
FspBI	CTAG	4 cut(s)	785, 839, 1116, 1509
GlaI	GCGC	4 cut(s)	121, 582, 1361, 1430
GluI	GCNGC	9 cut(s)	69, 72, 699, 702, 705, 891, 894, 1209, 1427
GsaI	CCCAGC	1 cut(s)	1344
GsuI	CTGGAG	1 cut(s)	963
HaeIII	GGCC	3 cut(s)	47, 419, 966
HapII	CCGG	6 cut(s)	23, 144, 186, 898, 1269, 1313
HgaI	GACGC	2 cut(s)	111, 1502
HhaI	GCGC	4 cut(s)	122, 583, 1362, 1431
Hin1I	GRCGYC	1 cut(s)	1513
Hin1II	CATG	7 cut(s)	542, 577, 784, 863, 872, 977, 1396
Hin6I	GCGC	4 cut(s)	120, 581, 1360, 1429
HinP1I	GCGC	4 cut(s)	120, 581, 1360, 1429
HincII	GTYRAC	1 cut(s)	175
HindII	GTYRAC	1 cut(s)	175
HinfI	GANTC	3 cut(s)	355, 788, 1364
HpaII	CCGG	6 cut(s)	23, 144, 186, 898, 1269, 1313
HphI	GGTGA	2 cut(s)	22, 37
Hpy166II	GTNNAC	4 cut(s)	175, 928, 1099, 1413
Hpy188I	TCNGA	6 cut(s)	98, 214, 451, 766, 1179, 1219
Hpy8I	GTNNAC	4 cut(s)	175, 928, 1099, 1413
Hpy99I	CGWCG	4 cut(s)	176, 179, 350, 1518
HpyAV	CCTTC	7 cut(s)	161, 212, 274, 456, 676, 1161, 1198
HpyCH4III	ACNGT	2 cut(s)	226, 1024
HpyCH4IV	ACGT	1 cut(s)	132
HpyCH4V	TGCA	8 cut(s)	545, 698, 704, 734, 859, 863, 1211, 1472
HpyF10VI	GCNNNNNNNGC	8 cut(s)	65, 91, 578, 611, 704, 953, 989, 1003
HpyF3I	CTNAG	9 cut(s)	55, 213, 359, 393, 486, 793, 1302, 1386, 1485
HpySE526I	ACGT	1 cut(s)	132
Hsp92I	GRCGYC	1 cut(s)	1513
Hsp92II	CATG	7 cut(s)	542, 577, 784, 863, 872, 977, 1396
HspAI	GCGC	4 cut(s)	120, 581, 1360, 1429
LguI	GCTCTTC	1 cut(s)	621
LmnI	GCTCC	4 cut(s)	887, 944, 953, 980
Lsp1109I	GCAGC	9 cut(s)	58, 80, 688, 710, 716, 877, 880, 1195, 1413
LweI	GCATC	8 cut(s)	532, 592, 681, 910, 917, 993, 1099, 1459
MaeI	CTAG	4 cut(s)	785, 839, 1116, 1509
MaeII	ACGT	1 cut(s)	132
MaeIII	GTNAC	3 cut(s)	10, 25, 1459
MbiI	CCGCTC	2 cut(s)	104, 128
MboII	GAAGA	7 cut(s)	259, 608, 738, 841, 962, 1069, 1300
MflI	RGATCY	3 cut(s)	803, 1171, 1526
MhlI	GDGCHC	5 cut(s)	54, 87, 96, 114, 958
MluCI	AATT	8 cut(s)	17, 386, 439, 473, 510, 630, 877, 1275
MmeI	TCCRAC	2 cut(s)	1144, 1322
MseI	TTAA	3 cut(s)	303, 999, 1274
MslI	CAYNNNNRTG	1 cut(s)	600
MspA1I	CMGCKG	4 cut(s)	71, 497, 701, 890
MspCI	CTTAAG	1 cut(s)	998
MspI	CCGG	6 cut(s)	23, 144, 186, 898, 1269, 1313
MspR9I	CCNGG	7 cut(s)	65, 144, 145, 206, 294, 898, 1313
Mva1269I	GAATGC	1 cut(s)	490
MvaI	CCWGG	3 cut(s)	65, 206, 294
MvnI	CGCG	4 cut(s)	36, 122, 1325, 1362
MwoI	GCNNNNNNNGC	8 cut(s)	65, 91, 578, 611, 704, 953, 989, 1003
NciI	CCSGG	4 cut(s)	144, 145, 898, 1313
NlaIII	CATG	7 cut(s)	542, 577, 784, 863, 872, 977, 1396
NlaIV	GGNNCC	6 cut(s)	100, 149, 159, 223, 687, 1173
NmuCI	GTSAC	2 cut(s)	10, 25
NruI	TCGCGA	1 cut(s)	1325
NspI	RCATGY	2 cut(s)	784, 863
PaeI	GCATGC	1 cut(s)	863
PaeR7I	CTCGAG	2 cut(s)	107, 831
PagI	TCATGA	2 cut(s)	538, 1392
PciSI	GCTCTTC	1 cut(s)	621
PcsI	WCGNNNNNNNCGW	2 cut(s)	345, 829
PctI	GAATGC	1 cut(s)	490
PfeI	GAWTC	3 cut(s)	355, 788, 1364
PinAI	ACCGGT	1 cut(s)	185
PkrI	GCNGC	9 cut(s)	70, 73, 700, 703, 706, 892, 895, 1210, 1428
PpuMI	RGGWCCY	1 cut(s)	158
PshAI	GACNNNNGTC	1 cut(s)	170
Psp124BI	GAGCTC	2 cut(s)	96, 958
Psp5II	RGGWCCY	1 cut(s)	158
Psp6I	CCWGG	3 cut(s)	63, 204, 292
PspFI	CCCAGC	1 cut(s)	1340
PspGI	CCWGG	3 cut(s)	63, 204, 292
PspN4I	GGNNCC	6 cut(s)	100, 149, 159, 223, 687, 1173
PspPI	GGNCC	5 cut(s)	98, 158, 183, 202, 965
PspPPI	RGGWCCY	1 cut(s)	158
PspXI	VCTCGAGB	1 cut(s)	831
PsrI	GAACNNNNNNTAC	2 cut(s)	761, 793
PstI	CTGCAG	2 cut(s)	706, 736
PstNI	CAGNNNCTG	1 cut(s)	893
PsuI	RGATCY	3 cut(s)	803, 1171, 1526
PvuII	CAGCTG	4 cut(s)	71, 497, 701, 890
RruI	TCGCGA	1 cut(s)	1325
RsaI	GTAC	4 cut(s)	403, 501, 561, 1100
RsaNI	GTAC	4 cut(s)	402, 500, 560, 1099
RseI	CAYNNNNRTG	1 cut(s)	600
SacI	GAGCTC	2 cut(s)	96, 958
SalI	GTCGAC	1 cut(s)	173
SapI	GCTCTTC	1 cut(s)	621
SaqAI	TTAA	3 cut(s)	303, 999, 1274
SatI	GCNGC	9 cut(s)	69, 72, 699, 702, 705, 891, 894, 1209, 1427
Sau96I	GGNCC	5 cut(s)	98, 158, 183, 202, 965
ScrFI	CCNGG	7 cut(s)	65, 144, 145, 206, 294, 898, 1313
SduI	GDGCHC	5 cut(s)	54, 87, 96, 114, 958
SfaNI	GCATC	8 cut(s)	532, 592, 681, 910, 917, 993, 1099, 1459
SfcI	CTRYAG	3 cut(s)	702, 732, 948
Sfr274I	CTCGAG	2 cut(s)	107, 831
SgrDI	CGTCGACG	1 cut(s)	173
SinI	GGWCC	4 cut(s)	98, 158, 183, 202
SlaI	CTCGAG	2 cut(s)	107, 831
SmaI	CCCGGG	1 cut(s)	145
SmiMI	CAYNNNNRTG	1 cut(s)	600
SmlI	CTYRAG	4 cut(s)	107, 242, 831, 998
SmoI	CTYRAG	4 cut(s)	107, 242, 831, 998
SphI	GCATGC	1 cut(s)	863
Sse9I	AATT	8 cut(s)	17, 386, 439, 473, 510, 630, 877, 1275
SsiI	CCGC	6 cut(s)	36, 102, 126, 140, 309, 915
SspMI	CTAG	4 cut(s)	785, 839, 1116, 1509
SstI	GAGCTC	2 cut(s)	96, 958
StyD4I	CCNGG	7 cut(s)	63, 142, 143, 204, 292, 896, 1311
StyI	CCWWGG	1 cut(s)	676
TaaI	ACNGT	2 cut(s)	226, 1024
TaiI	ACGT	1 cut(s)	135
TasI	AATT	8 cut(s)	17, 386, 439, 473, 510, 630, 877, 1275
TatI	WGTACW	1 cut(s)	1098
TfiI	GAWTC	3 cut(s)	355, 788, 1364
Tru1I	TTAA	3 cut(s)	303, 999, 1274
Tru9I	TTAA	3 cut(s)	303, 999, 1274
TscAI	CASTG	5 cut(s)	122, 241, 574, 937, 1361
TseFI	GTSAC	2 cut(s)	10, 25
TseI	GCWGC	9 cut(s)	68, 71, 698, 701, 704, 890, 893, 1208, 1426
Tsp45I	GTSAC	2 cut(s)	10, 25
TspDTI	ATGAA	4 cut(s)	841, 890, 962, 1033
TspGWI	ACGGA	1 cut(s)	29
TspMI	CCCGGG	1 cut(s)	143
TspRI	CASTG	5 cut(s)	122, 241, 574, 937, 1361
Vha464I	CTTAAG	1 cut(s)	998
VpaK11BI	GGWCC	4 cut(s)	98, 158, 183, 202
XagI	CCTNNNNNAGG	1 cut(s)	680
XapI	RAATTY	5 cut(s)	17, 439, 473, 510, 630
XceI	RCATGY	2 cut(s)	784, 863
XcmI	CCANNNNNNNNNTGG	1 cut(s)	823
XhoI	CTCGAG	2 cut(s)	107, 831
XmaI	CCCGGG	1 cut(s)	143
XmiI	GTMKAC	2 cut(s)	174, 1412
XspI	CTAG	4 cut(s)	785, 839, 1116, 1509

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.