Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002634

Physical Location & Seq

Genomic Coordinates Forward (+)

12458768 .. 12462907

Gene Structure 4140 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1156400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1545 bp

ATGTCGAGGGTGATGGAATTGCCGGTGACAGCTCGCGGCCGCTTAGCTGTGCTATCAGCGCACCTGGCAGCTGCGACCATCGAGCCCTATGAGTTCGGACCCGCTCTCGAGCCGCACTGCGTGTCCGCTCAGAACATGGTACCACCGCCCGGGAACCTCCGGGGACCTTTGACCATCGTCGACGAGAGGACCGGTAAGAGGTATCAGGTCCAGGTCTCTGATGAAGGAACCGTCAAGGCCACTGATCTCAAGAAGATCACAACAGGGAAAAATGACAAGGGGCTGAAGCTGTATGATCCAGGATATCTTAATACCGCCCCGGTTCGATCCTCCATTTGTTATATCGATGGCGACGAGGGGATTCTTAGATATAGAGGCTACCCAATTGAGGAATTGGCTGAGAATAGTACCTTTTTGGAAGTGGCCTATCTCTTGATGTATGGGAATTTGCCTTCTGAGAGTCAATATGCGGATTGGGAATTTGCCGTTTCTCAGCATTCAGCTGTACCGCAGGGAATTTTGGACATTATCCAGGCAATACCTCATGATGCACATCCAATGGGCGTACTTGTGAGTGCGATGAGTGCTCTTTCCGTTTTCCATCCTGATGCTAATCCTGCTCTCCGAGGGCAAGAAATTTACAAATCGAAACAAGTGAGAGACAAGCAAATAGCACGCATCCTTGGGAAGGCACCAACAATTGCAGCAGCAGCTTATTTGAGATTGGCTGGAAGACCTGCGGTTCTCCCTGCTAACAACCTTTCTTATTCAGAGAACTTTTTATACATGCTAGATTCCTTAGGCAATAGATCTTACAAACCGAATCCCCGACTGGCTCGAGTGTTAGATGTTCTCTTCATATTGCATGCAGAACATGAGATGAATTGCTCCACAGCTGCTGCCCGGCATCTGGCATCAAGTGGTGTAGATGTTTACACTGCTCTTGCTGGAGCTACAGGAGCTCTCTATGGCCCTCTTCATGGCGGAGCAAATGAGGCTGTGCTGAAGATGTTAAATGAGATTGGAACTGTTGAGAACATTCCAGATTTCATAGAGGGTGTGAAGAACAGGAAGCGAAAGATGTCAGGTTTTGGGCATCGCGTGTACAAAAACTATGATCCTAGAGCAAAGGTCATAAGGAAATTGGCAGAGGAAGTATTTTCCATTGTTGGAAGGGATCCTCTGATTGAGGTAGCTGTTGCTTTGGAGAAGGCTGCACTTTCAGATGAGTATTTTGCTAAGAGAAAGCTGTATCCAAATGTTGATTTCTACTCTGGATTAATCTACAGGGCAATGGGATTTCCAACGGAATTCTTCCCGGTTTTGTTCGCGATCCCTCGAATGGCTGGGTGGTTGGCACACTGGCGAGAATCCCTTGATGATCCTGATACTAAGATCATGAGACCCCAACAGGTGTATACTGGAAACTGGCTGCGCCACTACATACCGCCAAGAGAACGCATAGTACCAGGTGATGTAGACAAGCTCTCTCAGGTTTCTGTATCAAATGCCTCTAGGCGTCGTCTGGCCGGAACTGGTGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004108 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005911 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009506 GO:0009514 GO:0009987 GO:0016042 GO:0016054 GO:0016740 GO:0016746 GO:0019395 GO:0019752 GO:0030054 GO:0030258 GO:0032787 GO:0034440 GO:0036440 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0046912 GO:0055044 GO:0055114 GO:0071704 GO:0072329 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00020 ko00630 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Citrate_synt

Protein Analysis

515

Amino Acids

56.81

Weight (kDa)

8.79

Isoelectric Point (pI)

36.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Citrate_synt	PF00285	101 - 470	4.6e-136	Citrate synthase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002634)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42790 AT3G58740 AT3G58750
fragaria_vesca	FvH4_5g27540 FvH4_6g37750
malus_domestica	MD09G1156400.v1.1 MD17G1142900.v1.1
prunus_persica	Prupe.3G169400_v2.0.a1
pyrus_communis	pycom09g07510 pycom17g13590 pycom17g13600
rosa_chinensis	RchiOBHm_Chr2g0143111 RchiOBHm_Chr2g0150811 RchiOBHm_Chr7g0233461
rosa_laevigata	RLG00000020508
rosa_multiflora	Rmu_co8164368.1_g000001 Rmu_co8192116.1_g000001 Rmu_co8272387.1_g000001 Rmu_sc0003819.1_g000005 Rmu_sc0004794.1_g000003 Rmu_sc0020410.1_g000001
rosa_roxburghii	Rroxscaffold_2G00096940 Rroxscaffold_3G00251810
rosa_rugosa	Rorug02G0103600 Rorug02G0179700 Rorug02G0422600
rosa_samantha	Rh2AG024100 Rh2AG482700 Rh2BG495300 Rh2CG469000 Rh2DG450300 Rh2DG506100 Rh4CG229100 Rh4DG032400 Rh4DG200800 Rh5AG029300 Rh5CG031600 Rh5DG497900 Rh6CG201800
rosa_wichuraiana	Rw2G039560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	745
Acc65I	GGTACC	1 cut(s)	139
AccB1I	GGYRCC	2 cut(s)	139, 691
AccBSI	CCGCTC	2 cut(s)	104, 128
AccI	GTMKAC	3 cut(s)	180, 1418, 1479
AccII	CGCG	3 cut(s)	36, 1101, 1331
AclWI	GGATC	7 cut(s)	290, 321, 1112, 1172, 1185, 1327, 1376
AcoI	YGGCCR	2 cut(s)	37, 1527
AcsI	RAATTY	5 cut(s)	445, 479, 516, 636, 1310
AcuI	CTGAAG	2 cut(s)	305, 1025
AcyI	GRCGYC	1 cut(s)	1519
AdeI	CACNNNGTG	1 cut(s)	121
AfaI	GTAC	6 cut(s)	141, 409, 507, 567, 1106, 1467
AfiI	CCNNNNNNNGG	7 cut(s)	149, 198, 388, 688, 910, 980, 1342
AgeI	ACCGGT	1 cut(s)	191
AjnI	CCWGG	5 cut(s)	63, 210, 298, 531, 1468
Alw21I	GWGCWC	2 cut(s)	589, 964
Alw26I	GTCTC	3 cut(s)	220, 654, 1396
AlwI	GGATC	7 cut(s)	290, 321, 1112, 1172, 1185, 1327, 1376
AlwNI	CAGNNNCTG	1 cut(s)	899
Ama87I	CYCGRG	3 cut(s)	107, 149, 837
AoxI	GGCC	5 cut(s)	37, 237, 423, 970, 1527
ApeKI	GCWGC	9 cut(s)	68, 71, 704, 707, 710, 896, 899, 1214, 1432
ApoI	RAATTY	5 cut(s)	445, 479, 516, 636, 1310
AseI	ATTAAT	1 cut(s)	1280
AsiGI	ACCGGT	1 cut(s)	191
Asp718I	GGTACC	1 cut(s)	139
AspLEI	GCGC	2 cut(s)	61, 1437
AspS9I	GGNCC	5 cut(s)	98, 164, 189, 208, 971
AsuC2I	CCSGG	6 cut(s)	150, 151, 161, 320, 904, 1319
AsuHPI	GGTGA	3 cut(s)	22, 37, 1484
AvaI	CYCGRG	3 cut(s)	107, 149, 837
AvaII	GGWCC	4 cut(s)	98, 164, 189, 208
AxyI	CCTNAGG	1 cut(s)	799
BamHI	GGATCC	1 cut(s)	1177
BanI	GGYRCC	2 cut(s)	139, 691
BanII	GRGCYC	2 cut(s)	87, 964
BbsI	GAAGAC	1 cut(s)	739
Bbv12I	GWGCWC	2 cut(s)	589, 964
BbvI	GCAGC	9 cut(s)	58, 80, 716, 719, 722, 883, 886, 1201, 1419
BccI	CCATC	5 cut(s)	7, 86, 182, 341, 609
BceAI	ACGGC	1 cut(s)	470
BciT130I	CCWGG	5 cut(s)	65, 212, 300, 533, 1470
BciVI	GTATCC	1 cut(s)	1263
BcnI	CCSGG	6 cut(s)	150, 151, 161, 320, 904, 1319
BcoDI	GTCTC	3 cut(s)	220, 654, 1396
BfaI	CTAG	3 cut(s)	791, 1122, 1515
BfmI	CTRYAG	2 cut(s)	954, 1285
BfuAI	ACCTGC	1 cut(s)	745
BfuI	GTATCC	1 cut(s)	1263
BglII	AGATCT	1 cut(s)	809
BlpI	GCTNAGC	1 cut(s)	43
Bme18I	GGWCC	4 cut(s)	98, 164, 189, 208
BmeT110I	CYCGRG	3 cut(s)	107, 149, 837
BmgT120I	GGNCC	5 cut(s)	98, 164, 189, 208, 971
BmiI	GGNNCC	7 cut(s)	100, 141, 155, 165, 229, 693, 1179
BmsI	GCATC	6 cut(s)	538, 598, 687, 916, 923, 1105
BoxI	GACNNNNGTC	1 cut(s)	176
BpiI	GAAGAC	1 cut(s)	739
BpmI	CTGGAG	1 cut(s)	969
Bpu1102I	GCTNAGC	1 cut(s)	43
BpuEI	CTTGAG	1 cut(s)	233
BpuMI	CCSGG	6 cut(s)	150, 151, 161, 320, 904, 1319
Bsa29I	ATCGAT	1 cut(s)	345
BsaBI	GATNNNNATC	2 cut(s)	552, 612
BsaHI	GRCGYC	1 cut(s)	1519
BsaI	GGTCTC	2 cut(s)	220, 1396
BsaJI	CCNNGG	5 cut(s)	149, 160, 318, 625, 682
BsaWI	WCCGGW	1 cut(s)	191
Bsc4I	CCNNNNNNNGG	7 cut(s)	149, 198, 388, 688, 910, 980, 1342
Bse118I	RCCGGY	2 cut(s)	22, 191
Bse1I	ACTGG	5 cut(s)	837, 1367, 1426, 1433, 1540
Bse21I	CCTNAGG	1 cut(s)	799
Bse3DI	GCAATG	1 cut(s)	1299
Bse8I	GATNNNNATC	2 cut(s)	552, 612
BseBI	CCWGG	5 cut(s)	65, 212, 300, 533, 1470
BseCI	ATCGAT	1 cut(s)	345
BseDI	CCNNGG	5 cut(s)	149, 160, 318, 625, 682
BseGI	GGATG	3 cut(s)	553, 601, 678
BseJI	GATNNNNATC	2 cut(s)	552, 612
BseLI	CCNNNNNNNGG	7 cut(s)	149, 198, 388, 688, 910, 980, 1342
BseMI	GCAATG	1 cut(s)	1299
BseMII	CTCAG	5 cut(s)	143, 390, 447, 506, 1505
BseNI	ACTGG	5 cut(s)	837, 1367, 1426, 1433, 1540
BseX3I	CGGCCG	1 cut(s)	37
BseXI	GCAGC	9 cut(s)	58, 80, 716, 719, 722, 883, 886, 1201, 1419
BseYI	CCCAGC	1 cut(s)	1346
BsgI	GTGCAG	1 cut(s)	1200
Bsh1236I	CGCG	3 cut(s)	36, 1101, 1331
Bsh1285I	CGRYCG	1 cut(s)	40
BshFI	GGCC	5 cut(s)	39, 239, 425, 972, 1529
BshNI	GGYRCC	2 cut(s)	139, 691
BshTI	ACCGGT	1 cut(s)	191
BshVI	ATCGAT	1 cut(s)	345
BsiEI	CGRYCG	1 cut(s)	40
BsiHKAI	GWGCWC	2 cut(s)	589, 964
BsiHKCI	CYCGRG	3 cut(s)	107, 149, 837
BsiSI	CCGG	8 cut(s)	23, 150, 160, 192, 320, 904, 1319, 1530
BslFI	GGGAC	1 cut(s)	177
BslI	CCNNNNNNNGG	7 cut(s)	149, 198, 388, 688, 910, 980, 1342
BsmAI	GTCTC	3 cut(s)	220, 654, 1396
BsmFI	GGGAC	1 cut(s)	177
BsmI	GAATGC	1 cut(s)	496
BsnI	GGCC	5 cut(s)	39, 239, 425, 972, 1529
Bso31I	GGTCTC	2 cut(s)	220, 1396
BsoBI	CYCGRG	3 cut(s)	107, 149, 837
Bsp1286I	GDGCHC	3 cut(s)	87, 589, 964
Bsp1407I	TGTACA	1 cut(s)	1104
Bsp1720I	GCTNAGC	1 cut(s)	43
Bsp68I	TCGCGA	1 cut(s)	1331
BspANI	GGCC	5 cut(s)	39, 239, 425, 972, 1529
BspCNI	CTCAG	5 cut(s)	142, 391, 448, 505, 1504
BspDI	ATCGAT	1 cut(s)	345
BspFNI	CGCG	3 cut(s)	36, 1101, 1331
BspHI	TCATGA	2 cut(s)	544, 1398
BspLI	GGNNCC	7 cut(s)	100, 141, 155, 165, 229, 693, 1179
BspMI	ACCTGC	1 cut(s)	745
BspPI	GGATC	7 cut(s)	290, 321, 1112, 1172, 1185, 1327, 1376
BspT107I	GGYRCC	2 cut(s)	139, 691
BspTNI	GGTCTC	2 cut(s)	220, 1396
BsrBI	CCGCTC	2 cut(s)	104, 128
BsrDI	GCAATG	1 cut(s)	1299
BsrFI	RCCGGY	2 cut(s)	22, 191
BsrGI	TGTACA	1 cut(s)	1104
BsrI	ACTGG	5 cut(s)	837, 1367, 1426, 1433, 1540
BssAI	RCCGGY	2 cut(s)	22, 191
BssECI	CCNNGG	5 cut(s)	149, 160, 318, 625, 682
BssNAI	GTATAC	1 cut(s)	1419
BssNI	GRCGYC	1 cut(s)	1519
BssT1I	CCWWGG	1 cut(s)	682
Bst1107I	GTATAC	1 cut(s)	1419
Bst2UI	CCWGG	5 cut(s)	65, 212, 300, 533, 1470
Bst4CI	ACNGT	2 cut(s)	232, 1030
Bst6I	CTCTTC	2 cut(s)	860, 981
BstACI	GRCGYC	1 cut(s)	1519
BstAUI	TGTACA	1 cut(s)	1104
BstC8I	GCNNGC	3 cut(s)	34, 676, 867
BstENI	CCTNNNNNAGG	1 cut(s)	686
BstF5I	GGATG	3 cut(s)	553, 601, 678
BstFNI	CGCG	3 cut(s)	36, 1101, 1331
BstHHI	GCGC	2 cut(s)	61, 1437
BstMAI	GTCTC	3 cut(s)	220, 654, 1396
BstMCI	CGRYCG	1 cut(s)	40
BstMWI	GCNNNNNNNGC	7 cut(s)	58, 65, 584, 617, 710, 959, 995
BstNI	CCWGG	5 cut(s)	65, 212, 300, 533, 1470
BstNSI	RCATGY	2 cut(s)	790, 869
BstPAI	GACNNNNGTC	1 cut(s)	176
BstSFI	CTRYAG	2 cut(s)	954, 1285
BstUI	CGCG	3 cut(s)	36, 1101, 1331
BstV1I	GCAGC	9 cut(s)	58, 80, 716, 719, 722, 883, 886, 1201, 1419
BstV2I	GAAGAC	1 cut(s)	739
BstX2I	RGATCY	2 cut(s)	809, 1177
BstYI	RGATCY	2 cut(s)	809, 1177
BstZ17I	GTATAC	1 cut(s)	1419
BstZI	CGGCCG	1 cut(s)	37
Bsu15I	ATCGAT	1 cut(s)	345
Bsu36I	CCTNAGG	1 cut(s)	799
BsuI	GTATCC	1 cut(s)	1263
BsuRI	GGCC	5 cut(s)	39, 239, 425, 972, 1529
BsuTUI	ATCGAT	1 cut(s)	345
BtgZI	GCGATG	2 cut(s)	593, 1082
BtsCI	GGATG	3 cut(s)	553, 601, 678
BtsI	GCAGTG	2 cut(s)	115, 936
BtsIMutI	CAGTG	4 cut(s)	115, 240, 936, 1360
BtuMI	TCGCGA	1 cut(s)	1331
BveI	ACCTGC	1 cut(s)	745
Cac8I	GCNNGC	3 cut(s)	34, 676, 867
CaiI	CAGNNNCTG	1 cut(s)	899
CciI	TCATGA	2 cut(s)	544, 1398
CciNI	GCGGCCGC	1 cut(s)	37
CfoI	GCGC	2 cut(s)	61, 1437
Cfr10I	RCCGGY	2 cut(s)	22, 191
Cfr13I	GGNCC	5 cut(s)	98, 164, 189, 208, 971
Cfr9I	CCCGGG	1 cut(s)	149
ClaI	ATCGAT	1 cut(s)	345
CseI	GACGC	1 cut(s)	1508
CsiI	ACCWGGT	1 cut(s)	1468
Csp6I	GTAC	6 cut(s)	140, 408, 506, 566, 1105, 1466
CspAI	ACCGGT	1 cut(s)	191
CviAII	CATG	7 cut(s)	136, 545, 787, 866, 875, 980, 1399
CviQI	GTAC	6 cut(s)	140, 408, 506, 566, 1105, 1466
DraIII	CACNNNGTG	1 cut(s)	121
EaeI	YGGCCR	2 cut(s)	37, 1527
EagI	CGGCCG	1 cut(s)	37
Eam1104I	CTCTTC	2 cut(s)	860, 981
EarI	CTCTTC	2 cut(s)	860, 981
EciI	GGCGGA	1 cut(s)	999
Ecl136II	GAGCTC	1 cut(s)	962
EclXI	CGGCCG	1 cut(s)	37
Eco130I	CCWWGG	1 cut(s)	682
Eco24I	GRGCYC	2 cut(s)	87, 964
Eco31I	GGTCTC	2 cut(s)	220, 1396
Eco32I	GATATC	1 cut(s)	305
Eco47I	GGWCC	4 cut(s)	98, 164, 189, 208
Eco52I	CGGCCG	1 cut(s)	37
Eco53kI	GAGCTC	1 cut(s)	962
Eco57I	CTGAAG	2 cut(s)	305, 1025
Eco81I	CCTNAGG	1 cut(s)	799
Eco88I	CYCGRG	3 cut(s)	107, 149, 837
EcoICRI	GAGCTC	1 cut(s)	962
EcoNI	CCTNNNNNAGG	1 cut(s)	686
EcoO109I	RGGNCCY	1 cut(s)	164
EcoRI	GAATTC	1 cut(s)	1310
EcoRII	CCWGG	5 cut(s)	63, 210, 298, 531, 1468
EcoRV	GATATC	1 cut(s)	305
EcoT14I	CCWWGG	1 cut(s)	682
EcoT38I	GRGCYC	2 cut(s)	87, 964
ErhI	CCWWGG	1 cut(s)	682
FaeI	CATG	7 cut(s)	139, 548, 790, 869, 878, 983, 1402
FaqI	GGGAC	1 cut(s)	177
FatI	CATG	7 cut(s)	135, 544, 786, 865, 874, 979, 1398
FauI	CCCGC	1 cut(s)	109
FblI	GTMKAC	3 cut(s)	180, 1418, 1479
FokI	GGATG	3 cut(s)	540, 588, 665
FriOI	GRGCYC	2 cut(s)	87, 964
FspBI	CTAG	3 cut(s)	791, 1122, 1515
GlaI	GCGC	2 cut(s)	60, 1436
GsaI	CCCAGC	1 cut(s)	1350
GsuI	CTGGAG	1 cut(s)	969
HaeIII	GGCC	5 cut(s)	39, 239, 425, 972, 1529
HapII	CCGG	8 cut(s)	23, 150, 160, 192, 320, 904, 1319, 1530
HgaI	GACGC	1 cut(s)	1508
HhaI	GCGC	2 cut(s)	61, 1437
Hin1I	GRCGYC	1 cut(s)	1519
Hin1II	CATG	7 cut(s)	139, 548, 790, 869, 878, 983, 1402
Hin6I	GCGC	2 cut(s)	59, 1435
HinP1I	GCGC	2 cut(s)	59, 1435
HincII	GTYRAC	1 cut(s)	181
HindII	GTYRAC	1 cut(s)	181
HinfI	GANTC	5 cut(s)	361, 460, 794, 823, 1370
HpaII	CCGG	8 cut(s)	23, 150, 160, 192, 320, 904, 1319, 1530
HphI	GGTGA	3 cut(s)	22, 37, 1484
Hpy166II	GTNNAC	5 cut(s)	181, 934, 1105, 1419, 1480
Hpy188I	TCNGA	8 cut(s)	98, 132, 220, 457, 626, 772, 1185, 1225
Hpy8I	GTNNAC	5 cut(s)	181, 934, 1105, 1419, 1480
Hpy99I	CGWCG	4 cut(s)	182, 185, 356, 1524
HpyAV	CCTTC	5 cut(s)	218, 462, 682, 1167, 1204
HpyCH4III	ACNGT	2 cut(s)	232, 1030
HpyCH4V	TGCA	5 cut(s)	551, 704, 865, 869, 1217
HpyF10VI	GCNNNNNNNGC	7 cut(s)	58, 65, 584, 617, 710, 959, 995
Hsp92I	GRCGYC	1 cut(s)	1519
Hsp92II	CATG	7 cut(s)	139, 548, 790, 869, 878, 983, 1402
HspAI	GCGC	2 cut(s)	59, 1435
KpnI	GGTACC	1 cut(s)	143
LmnI	GCTCC	4 cut(s)	893, 950, 959, 986
Lsp1109I	GCAGC	9 cut(s)	58, 80, 716, 719, 722, 883, 886, 1201, 1419
LweI	GCATC	6 cut(s)	538, 598, 687, 916, 923, 1105
MabI	ACCWGGT	1 cut(s)	1468
MaeI	CTAG	3 cut(s)	791, 1122, 1515
MaeIII	GTNAC	1 cut(s)	25
MbiI	CCGCTC	2 cut(s)	104, 128
MboII	GAAGA	7 cut(s)	265, 744, 847, 968, 1018, 1075, 1306
MfeI	CAATTG	2 cut(s)	384, 699
MflI	RGATCY	2 cut(s)	809, 1177
MhlI	GDGCHC	3 cut(s)	87, 589, 964
MlyI	GAGTC	1 cut(s)	469
MmeI	TCCRAC	2 cut(s)	1150, 1328
MseI	TTAA	3 cut(s)	309, 1013, 1280
MslI	CAYNNNNRTG	1 cut(s)	606
MspA1I	CMGCKG	3 cut(s)	71, 503, 896
MspI	CCGG	8 cut(s)	23, 150, 160, 192, 320, 904, 1319, 1530
MunI	CAATTG	2 cut(s)	384, 699
Mva1269I	GAATGC	1 cut(s)	496
MvaI	CCWGG	5 cut(s)	65, 212, 300, 533, 1470
MvnI	CGCG	3 cut(s)	36, 1101, 1331
MwoI	GCNNNNNNNGC	7 cut(s)	58, 65, 584, 617, 710, 959, 995
NciI	CCSGG	6 cut(s)	150, 151, 161, 320, 904, 1319
NlaIII	CATG	7 cut(s)	139, 548, 790, 869, 878, 983, 1402
NlaIV	GGNNCC	7 cut(s)	100, 141, 155, 165, 229, 693, 1179
NmuCI	GTSAC	1 cut(s)	25
NotI	GCGGCCGC	1 cut(s)	37
NruI	TCGCGA	1 cut(s)	1331
NspI	RCATGY	2 cut(s)	790, 869
PaeI	GCATGC	1 cut(s)	869
PaeR7I	CTCGAG	2 cut(s)	107, 837
PagI	TCATGA	2 cut(s)	544, 1398
PcsI	WCGNNNNNNNCGW	1 cut(s)	351
PctI	GAATGC	1 cut(s)	496
PfeI	GAWTC	4 cut(s)	361, 794, 823, 1370
PfoI	TCCNGGA	1 cut(s)	298
PinAI	ACCGGT	1 cut(s)	191
PleI	GAGTC	1 cut(s)	468
PpsI	GAGTC	1 cut(s)	468
PpuMI	RGGWCCY	1 cut(s)	164
PshAI	GACNNNNGTC	1 cut(s)	176
PshBI	ATTAAT	1 cut(s)	1280
Psp124BI	GAGCTC	1 cut(s)	964
Psp5II	RGGWCCY	1 cut(s)	164
Psp6I	CCWGG	5 cut(s)	63, 210, 298, 531, 1468
PspFI	CCCAGC	1 cut(s)	1346
PspGI	CCWGG	5 cut(s)	63, 210, 298, 531, 1468
PspN4I	GGNNCC	7 cut(s)	100, 141, 155, 165, 229, 693, 1179
PspPI	GGNCC	5 cut(s)	98, 164, 189, 208, 971
PspPPI	RGGWCCY	1 cut(s)	164
PspXI	VCTCGAGB	1 cut(s)	837
PsrI	GAACNNNNNNTAC	4 cut(s)	767, 799, 1449, 1481
PstNI	CAGNNNCTG	1 cut(s)	899
PsuI	RGATCY	2 cut(s)	809, 1177
PvuII	CAGCTG	3 cut(s)	71, 503, 896
RruI	TCGCGA	1 cut(s)	1331
RsaI	GTAC	6 cut(s)	141, 409, 507, 567, 1106, 1467
RsaNI	GTAC	6 cut(s)	140, 408, 506, 566, 1105, 1466
RseI	CAYNNNNRTG	1 cut(s)	606
SacI	GAGCTC	1 cut(s)	964
SalI	GTCGAC	1 cut(s)	179
SaqAI	TTAA	3 cut(s)	309, 1013, 1280
Sau96I	GGNCC	5 cut(s)	98, 164, 189, 208, 971
SchI	GAGTC	1 cut(s)	469
SduI	GDGCHC	3 cut(s)	87, 589, 964
SexAI	ACCWGGT	1 cut(s)	1468
SfaNI	GCATC	6 cut(s)	538, 598, 687, 916, 923, 1105
SfcI	CTRYAG	2 cut(s)	954, 1285
Sfr274I	CTCGAG	2 cut(s)	107, 837
SgrDI	CGTCGACG	1 cut(s)	179
SinI	GGWCC	4 cut(s)	98, 164, 189, 208
SlaI	CTCGAG	2 cut(s)	107, 837
SmaI	CCCGGG	1 cut(s)	151
SmiMI	CAYNNNNRTG	1 cut(s)	606
SmlI	CTYRAG	3 cut(s)	107, 248, 837
SmoI	CTYRAG	3 cut(s)	107, 248, 837
SphI	GCATGC	1 cut(s)	869
SspMI	CTAG	3 cut(s)	791, 1122, 1515
SstI	GAGCTC	1 cut(s)	964
StyI	CCWWGG	1 cut(s)	682
TaaI	ACNGT	2 cut(s)	232, 1030
TaqI	TCGA	9 cut(s)	5, 81, 108, 180, 325, 345, 647, 838, 1339
TatI	WGTACW	1 cut(s)	1104
TauI	GCSGC	3 cut(s)	39, 42, 115
TfiI	GAWTC	4 cut(s)	361, 794, 823, 1370
Tru1I	TTAA	3 cut(s)	309, 1013, 1280
Tru9I	TTAA	3 cut(s)	309, 1013, 1280
TscAI	CASTG	4 cut(s)	122, 247, 943, 1367
TseFI	GTSAC	1 cut(s)	25
TseI	GCWGC	9 cut(s)	68, 71, 704, 707, 710, 896, 899, 1214, 1432
Tsp45I	GTSAC	1 cut(s)	25
TspDTI	ATGAA	5 cut(s)	237, 847, 896, 968, 1039
TspGWI	ACGGA	2 cut(s)	583, 1322
TspMI	CCCGGG	1 cut(s)	149
TspRI	CASTG	4 cut(s)	122, 247, 943, 1367
VpaK11BI	GGWCC	4 cut(s)	98, 164, 189, 208
VspI	ATTAAT	1 cut(s)	1280
XagI	CCTNNNNNAGG	1 cut(s)	686
XapI	RAATTY	5 cut(s)	445, 479, 516, 636, 1310
XceI	RCATGY	2 cut(s)	790, 869
XhoI	CTCGAG	2 cut(s)	107, 837
XmaI	CCCGGG	1 cut(s)	149
XmiI	GTMKAC	3 cut(s)	180, 1418, 1479
XspI	CTAG	3 cut(s)	791, 1122, 1515

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.