Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0002634

Physical Location & Seq

Genomic Coordinates Forward (+)

18259830 .. 18267579

Gene Structure 7750 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00096940.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2547 bp

ATGGCCATGACTTGGAAGCTCCTGAAGCAACTCCGACCACCACCGTACACCGCCACCGCCTCTACGAGCCTCCACCGCCCTCGATTTCTCAACTTCCATAATGCCACTCGGCTCTTCTCCGATGCGCCTCCTCCCTCCGGAGAGCCTCCGCCGATTCCACCGCCTCAAAACTCTCACTCTCCCTCGCCGTCGTCGCTGAACCAGGCAGAGCTCGCTAAGTTCGCCGCCATTGCCGGAACCTGGTGGGATTCTGAAGGACCGTTCAAACCTTTGCATGCGTTGAATCCTACAAGGCTCGCCTTCATTCGCTCAACGCTGTGTCGTCATTTCAGGAGGGATCCAGTATCTGCTAGGCCATTTGAAGGGCTCAAGTTTATTGATGTTGGGTGTGGTGGTGGAATTCTGTCTGAGCCTTTAGCACGCATGGGAGCTTCTGTGACTGGAGTTGATGCTGTTGAGAAAAATATCAGGATAGCTCGCCTTCATGCTGACTTAGATCCAGTGACCTCAAGCATTGAATATCATTGCACAACAGCTGAAAAATTAGTGGAGGAGCAGAGGAGTTTTGATGCTGTGTTTGCTCTAGAGGTAATTGAGCACGTGGCGAATCCTGCTGAGTTCTGCAAATCTCTGTCTGCCTTAACTGTTCCTGATGGAGCTACTGTGATTTCAACTATTAACCGTTCTATGAGAGCATATGCTACTGCAATTATTGCTGCCGAGTATCTCCTCCACTGGCTTCCTAAGGGTACACATCAATGGTCTAGTTTCCTTACCCCTGAAGAACTAGTCTTGATCCTGGAGCGTGCTTCTATTTCGGTCCAAGAGATGGCTGGATTTGCATACAACCCTTTGACAGGAAGATGGTCTATATCTGATGATGTTAGTGTAAACTTTATCGCTTATGTGAAATCTGGTGTCACTGATCTGATCGATAAAGAAAGGAAGAAGAAGCAGAAACGTCTCTCTTCTCCTCTCTATAAGCTCCGTGACGTTTTCAATTTGATTTATAGAAATAGTAACATGTCGAGTGAATTTCCGGTAAGAGCTCGTGGCCGCTTGGCCGTGCTTTCGGCCCACCTCGCCGCCGCAACCATCTCCGGGTCCTCGGAGCTCGAGCCTCAGTGCTTGTCCGCCCAGGTGCCGCCTCCGGCTAACCTCCGCGGCGCTTTGACCGTCGTTGACGAGAGGACCGGTAAGCGCTACCAGGTCCAGGTCTCCGAACAAGGAACCGTCAAGGCCACTGATCTCAAAAAGATTAGTACGGGGAAGAATGATAAGGGGCTGAAGCTGTATGATCCGGGGTATCTCAACACTGCGCCGGTTCGATCTTCGATTTGTTATATTGATGGAGATGAGGGGATTCTTAGGTACAGAGGGTATCCAATTGAGGAATTGGCTGAGAGTAGTACTTATTTGGAAGTGGCATATCTCCTCATGTATGGGAATTTGCCGTCTCAAAGCCAATTAGCGGATTGGGAATTTGCCGTTACTCAGCATTCAGCTGTACCACAGGGGATTTTGGATATTATACAAGCAATACCTCATGATGCACATCCAATGGGTGTACTTGTCAGCGCAATGAGTGCTCTTTCTGTTTTTCACCCTGATGCCAATCCTGCCCTCAGAGGGCAAGACCTTTACCAGTCCAAACAAGTGAGAGACAAACAAATTGCACGGATTCTTGGGAAGGCACCAACTATTGCAGCAGCAGCTTATTTGAGATTGGCAGGAAGACCCCCAGTTCTCCCATCTAACAACCTTTCTTATTCCGAGAACTTCTTATACATGCTAGACTCCTTGGGCAATAGAGCTTATAAACCAAATCCCCGCCTCGCCCGAGTATTGGACATTCTCTTCATACTCCACGCTGAACATGAGATGAATTGCTCAACGGCTGCTGCTCGGCATCTGGCGTCAAGTGGTGTGGATGTTTACACTGCCCTTTCTGGAGCTGTTGGAGCTCTATATGGTCCCCTTCATGGTGGAGCAAATGAGGCTGTGCTTAAGATGCTAAGTGAAATTGGATCTGTTGAGAACATTCCAGAGTTCATTGAGGGTGTGAAGAGCAGGAAGCGAAAGATGTCAGGTTTTGGGCATCGCGTGTACAAGAACTATGATCCTAGAGCAAAGGTCATAAGAAAGCTGGCAGATGAAGTATTCTCCATTGTTGGAAGGGATCCACTGATTGAGGTTGCTGTTGCTTTGGAGAAAGCTGCACTATCTGATGAATATTTTGTTAAGAGAAAGCTGTATCCTAATGTTGATTTCTACTCCGGGTTGATATATAGGGCAATGGGATTTCCAACGGAGTTCTTCCCAGTTTTATTTGCAATCCCTAGAATGGCTGGTTACTTGGCACACTGGAGAGAATCCCTGGATGATCCTGATACTAAGATTATGAGGCCCGCACAGGTATATGTTGGGGAGTGGCTGCGGCACTATATGCCATTGAAAGAACGGATAGTGAGCACTGATTCAGACAAACTATCTCAAGTTTCTGTTTCAAATGCCTCCAAGCGTCGTTTGTCTGGATCTGGAGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004108 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005911 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009506 GO:0009514 GO:0009987 GO:0016042 GO:0016054 GO:0016740 GO:0016746 GO:0019395 GO:0019752 GO:0030054 GO:0030258 GO:0032787 GO:0034440 GO:0036440 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0046912 GO:0055044 GO:0055114 GO:0071704 GO:0072329 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00020 ko00630 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Citrate_synt

Protein Analysis

848

Amino Acids

93.6

Weight (kDa)

8.99

Isoelectric Point (pI)

42.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_23	PF13489	114 - 270	2.5e-19	Methyltransferase domain
Methyltransf_31	PF13847	121 - 251	4.7e-13	Methyltransferase domain
PrmA	PF06325	124 - 226	9.4e-07	Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_11	PF08241	126 - 222	3.5e-18	Methyltransferase domain
Methyltransf_25	PF13649	126 - 219	1.9e-14	Methyltransferase domain
Methyltransf_12	PF08242	126 - 219	4.4e-11	Methyltransferase domain
Citrate_synt	PF00285	435 - 804	2.2e-134	Citrate synthase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002634)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42790 AT3G58740 AT3G58750
fragaria_vesca	FvH4_5g27540 FvH4_6g37750
malus_domestica	MD09G1156400.v1.1 MD17G1142900.v1.1
prunus_persica	Prupe.3G169400_v2.0.a1
pyrus_communis	pycom09g07510 pycom17g13590 pycom17g13600
rosa_chinensis	RchiOBHm_Chr2g0143111 RchiOBHm_Chr2g0150811 RchiOBHm_Chr7g0233461
rosa_laevigata	RLG00000020508
rosa_multiflora	Rmu_co8164368.1_g000001 Rmu_co8192116.1_g000001 Rmu_co8272387.1_g000001 Rmu_sc0003819.1_g000005 Rmu_sc0004794.1_g000003 Rmu_sc0020410.1_g000001
rosa_roxburghii	Rroxscaffold_2G00096940 Rroxscaffold_3G00251810
rosa_rugosa	Rorug02G0103600 Rorug02G0179700 Rorug02G0422600
rosa_samantha	Rh2AG024100 Rh2AG482700 Rh2BG495300 Rh2CG469000 Rh2DG450300 Rh2DG506100 Rh4CG229100 Rh4DG032400 Rh4DG200800 Rh5AG029300 Rh5CG031600 Rh5DG497900 Rh6CG201800
rosa_wichuraiana	Rw2G039560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1818
AccB1I	GGYRCC	2 cut(s)	1141, 1693
AccB7I	CCANNNNNTGG	2 cut(s)	12, 829
AccII	CGCG	2 cut(s)	1164, 2103
AccIII	TCCGGA	1 cut(s)	137
AcoI	YGGCCR	3 cut(s)	3, 1054, 1062
AcsI	RAATTY	4 cut(s)	399, 1034, 1447, 1481
AcuI	CTGAAG	4 cut(s)	44, 273, 801, 1307
AcvI	CACGTG	1 cut(s)	601
AcyI	GRCGYC	1 cut(s)	1916
AfaI	GTAC	8 cut(s)	47, 751, 1264, 1373, 1411, 1509, 1569, 2108
AfeI	AGCGCT	1 cut(s)	1202
AflII	CTTAAG	1 cut(s)	2006
AflIII	ACRYGT	1 cut(s)	1023
AgeI	ACCGGT	1 cut(s)	1193
AgsI	TTSAA	8 cut(s)	265, 283, 362, 518, 672, 1000, 2455, 2508
AhlI	ACTAGT	1 cut(s)	787
AjnI	CCWGG	7 cut(s)	201, 239, 798, 1137, 1206, 1212, 2376
Alw21I	GWGCWC	7 cut(s)	213, 600, 1051, 1116, 1591, 1966, 2474
Alw26I	GTCTC	4 cut(s)	968, 1222, 1461, 1656
AlwNI	CAGNNNCTG	1 cut(s)	347
Ama87I	CYCGRG	2 cut(s)	1115, 1839
Aor13HI	TCCGGA	1 cut(s)	137
Aor51HI	AGCGCT	1 cut(s)	1202
AoxI	GGCC	7 cut(s)	3, 353, 1054, 1062, 1074, 1239, 2405
ApeKI	GCWGC	8 cut(s)	716, 1706, 1709, 1712, 1898, 1901, 2216, 2434
ApoI	RAATTY	4 cut(s)	399, 1034, 1447, 1481
AsiGI	ACCGGT	1 cut(s)	1193
AspLEI	GCGC	5 cut(s)	127, 1169, 1203, 1321, 1580
AspS9I	GGNCC	8 cut(s)	257, 820, 1075, 1104, 1191, 1210, 1973, 2406
AsuC2I	CCSGG	3 cut(s)	1102, 1302, 2278
AsuHPI	GGTGA	1 cut(s)	1595
AvaI	CYCGRG	2 cut(s)	1115, 1839
AvaII	GGWCC	6 cut(s)	257, 820, 1104, 1191, 1210, 1973
AxyI	CCTNAGG	1 cut(s)	744
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	2 cut(s)	337, 2179
BanI	GGYRCC	2 cut(s)	1141, 1693
BanII	GRGCYC	5 cut(s)	213, 369, 1051, 1116, 1966
BauI	CACGAG	1 cut(s)	1050
BbrPI	CACGTG	1 cut(s)	601
BbsI	GAAGAC	1 cut(s)	1741
Bbv12I	GWGCWC	7 cut(s)	213, 600, 1051, 1116, 1591, 1966, 2474
BbvI	GCAGC	8 cut(s)	703, 1718, 1721, 1724, 1885, 1888, 2203, 2421
BccI	CCATC	6 cut(s)	647, 823, 858, 1103, 1343, 1759
BceAI	ACGGC	5 cut(s)	172, 1049, 1438, 1472, 1911
BciT130I	CCWGG	7 cut(s)	203, 241, 800, 1139, 1208, 1214, 2378
BciVI	GTATCC	2 cut(s)	1392, 2265
BcnI	CCSGG	3 cut(s)	1102, 1302, 2278
BcoDI	GTCTC	4 cut(s)	968, 1222, 1461, 1656
BcuI	ACTAGT	1 cut(s)	787
BfaI	CTAG	7 cut(s)	351, 584, 765, 788, 1793, 2124, 2340
BfoI	RGCGCY	2 cut(s)	1170, 1204
BfrI	CTTAAG	1 cut(s)	2006
BfuI	GTATCC	2 cut(s)	1392, 2265
BmcAI	AGTACT	1 cut(s)	1411
Bme18I	GGWCC	6 cut(s)	257, 820, 1104, 1191, 1210, 1973
BmeT110I	CYCGRG	2 cut(s)	1115, 1839
BmgT120I	GGNCC	8 cut(s)	257, 820, 1075, 1104, 1191, 1210, 1973, 2406
BmiI	GGNNCC	8 cut(s)	238, 339, 1105, 1143, 1231, 1695, 1975, 2181
BmrI	ACTGGG	2 cut(s)	1736, 2315
BmsI	GCATC	8 cut(s)	112, 439, 559, 1540, 1600, 1918, 2001, 2107
BmuI	ACTGGG	2 cut(s)	1736, 2315
BpiI	GAAGAC	1 cut(s)	1741
BpmI	CTGGAG	4 cut(s)	462, 821, 1971, 2386
BpuEI	CTTGAG	3 cut(s)	353, 493, 2478
BpuMI	CCSGG	3 cut(s)	1102, 1302, 2278
Bsa29I	ATCGAT	1 cut(s)	933
BsaAI	YACGTR	1 cut(s)	601
BsaBI	GATNNNNATC	2 cut(s)	1554, 1614
BsaHI	GRCGYC	1 cut(s)	1916
BsaI	GGTCTC	1 cut(s)	1222
BsaJI	CCNNGG	6 cut(s)	1107, 1137, 1162, 1301, 1800, 2376
BsaWI	WCCGGW	3 cut(s)	137, 1039, 1193
BsaXI	ACNNNNNCTCC	4 cut(s)	420, 450, 648, 678
Bse118I	RCCGGY	2 cut(s)	1193, 1321
Bse1I	ACTGG	8 cut(s)	341, 445, 500, 740, 1645, 1742, 2321, 2369
Bse21I	CCTNAGG	1 cut(s)	744
Bse3DI	GCAATG	4 cut(s)	228, 523, 1587, 2301
Bse8I	GATNNNNATC	2 cut(s)	1554, 1614
BseAI	TCCGGA	1 cut(s)	137
BseBI	CCWGG	7 cut(s)	203, 241, 800, 1139, 1208, 1214, 2378
BseCI	ATCGAT	1 cut(s)	933
BseDI	CCNNGG	6 cut(s)	1107, 1137, 1162, 1301, 1800, 2376
BseGI	GGATG	3 cut(s)	1555, 1936, 2386
BseJI	GATNNNNATC	2 cut(s)	1554, 1614
BseMI	GCAATG	4 cut(s)	228, 523, 1587, 2301
BseMII	CTCAG	6 cut(s)	399, 606, 1136, 1392, 1508, 1639
BseNI	ACTGG	8 cut(s)	341, 445, 500, 740, 1645, 1742, 2321, 2369
BseRI	GAGGAG	6 cut(s)	120, 566, 574, 719, 963, 1424
BseXI	GCAGC	8 cut(s)	703, 1718, 1721, 1724, 1885, 1888, 2203, 2421
BsgI	GTGCAG	1 cut(s)	2202
Bsh1236I	CGCG	2 cut(s)	1164, 2103
BshFI	GGCC	7 cut(s)	5, 355, 1056, 1064, 1076, 1241, 2407
BshNI	GGYRCC	2 cut(s)	1141, 1693
BshTI	ACCGGT	1 cut(s)	1193
BshVI	ATCGAT	1 cut(s)	933
BsiHKAI	GWGCWC	7 cut(s)	213, 600, 1051, 1116, 1591, 1966, 2474
BsiHKCI	CYCGRG	2 cut(s)	1115, 1839
BsiSI	CCGG	9 cut(s)	138, 234, 1040, 1101, 1151, 1194, 1301, 1322, 2277
BslFI	GGGAC	1 cut(s)	1959
BsmAI	GTCTC	4 cut(s)	968, 1222, 1461, 1656
BsmBI	CGTCTC	2 cut(s)	968, 1461
BsmFI	GGGAC	1 cut(s)	1959
BsmI	GAATGC	1 cut(s)	1498
BsnI	GGCC	7 cut(s)	5, 355, 1056, 1064, 1076, 1241, 2407
Bso31I	GGTCTC	1 cut(s)	1222
BsoBI	CYCGRG	2 cut(s)	1115, 1839
Bsp1286I	GDGCHC	8 cut(s)	213, 369, 600, 1051, 1116, 1591, 1966, 2474
Bsp13I	TCCGGA	1 cut(s)	137
Bsp1407I	TGTACA	1 cut(s)	2106
BspANI	GGCC	7 cut(s)	5, 355, 1056, 1064, 1076, 1241, 2407
BspCNI	CTCAG	6 cut(s)	400, 607, 1135, 1393, 1507, 1638
BspDI	ATCGAT	1 cut(s)	933
BspEI	TCCGGA	1 cut(s)	137
BspFNI	CGCG	2 cut(s)	1164, 2103
BspHI	TCATGA	1 cut(s)	1546
BspLI	GGNNCC	8 cut(s)	238, 339, 1105, 1143, 1231, 1695, 1975, 2181
BspQI	GCTCTTC	2 cut(s)	119, 2060
BspT107I	GGYRCC	2 cut(s)	1141, 1693
BspTI	CTTAAG	1 cut(s)	2006
BspTNI	GGTCTC	1 cut(s)	1222
BsrDI	GCAATG	4 cut(s)	228, 523, 1587, 2301
BsrFI	RCCGGY	2 cut(s)	1193, 1321
BsrGI	TGTACA	1 cut(s)	2106
BsrI	ACTGG	8 cut(s)	341, 445, 500, 740, 1645, 1742, 2321, 2369
BssAI	RCCGGY	2 cut(s)	1193, 1321
BssECI	CCNNGG	6 cut(s)	1107, 1137, 1162, 1301, 1800, 2376
BssNI	GRCGYC	1 cut(s)	1916
BssSI	CACGAG	1 cut(s)	1050
BssT1I	CCWWGG	1 cut(s)	1800
Bst2BI	CACGAG	1 cut(s)	1050
Bst2UI	CCWGG	7 cut(s)	203, 241, 800, 1139, 1208, 1214, 2378
Bst4CI	ACNGT	7 cut(s)	45, 261, 646, 664, 683, 1177, 1234
Bst6I	CTCTTC	4 cut(s)	119, 973, 1862, 2060
BstACI	GRCGYC	1 cut(s)	1916
BstAFI	CTTAAG	1 cut(s)	2006
BstAPI	GCANNNNNTGC	3 cut(s)	713, 1586, 2446
BstAUI	TGTACA	1 cut(s)	2106
BstBAI	YACGTR	1 cut(s)	601
BstC8I	GCNNGC	8 cut(s)	213, 276, 297, 421, 478, 807, 2148, 2409
BstDSI	CCRYGG	1 cut(s)	1162
BstENI	CCTNNNNNAGG	1 cut(s)	855
BstF5I	GGATG	3 cut(s)	1555, 1936, 2386
BstFNI	CGCG	2 cut(s)	1164, 2103
BstH2I	RGCGCY	2 cut(s)	1170, 1204
BstHHI	GCGC	5 cut(s)	127, 1169, 1203, 1321, 1580
BstMAI	GTCTC	4 cut(s)	968, 1222, 1461, 1656
BstNI	CCWGG	7 cut(s)	203, 241, 800, 1139, 1208, 1214, 2378
BstNSI	RCATGY	3 cut(s)	278, 1027, 1792
BstUI	CGCG	2 cut(s)	1164, 2103
BstV1I	GCAGC	8 cut(s)	703, 1718, 1721, 1724, 1885, 1888, 2203, 2421
BstV2I	GAAGAC	1 cut(s)	1741
BstX2I	RGATCY	5 cut(s)	337, 496, 2027, 2179, 2534
BstYI	RGATCY	5 cut(s)	337, 496, 2027, 2179, 2534
Bsu15I	ATCGAT	1 cut(s)	933
Bsu36I	CCTNAGG	1 cut(s)	744
BsuI	GTATCC	2 cut(s)	1392, 2265
BsuRI	GGCC	7 cut(s)	5, 355, 1056, 1064, 1076, 1241, 2407
BsuTUI	ATCGAT	1 cut(s)	933
BtgI	CCRYGG	1 cut(s)	1162
BtgZI	GCGATG	1 cut(s)	2084
BtsCI	GGATG	3 cut(s)	1555, 1936, 2386
BtsI	GCAGTG	2 cut(s)	1314, 1938
Cac8I	GCNNGC	8 cut(s)	213, 276, 297, 421, 478, 807, 2148, 2409
CaiI	CAGNNNCTG	1 cut(s)	347
CciI	TCATGA	1 cut(s)	1546
CfoI	GCGC	5 cut(s)	127, 1169, 1203, 1321, 1580
Cfr10I	RCCGGY	2 cut(s)	1193, 1321
Cfr13I	GGNCC	8 cut(s)	257, 820, 1075, 1104, 1191, 1210, 1973, 2406
Cfr42I	CCGCGG	1 cut(s)	1165
ClaI	ATCGAT	1 cut(s)	933
CseI	GACGC	2 cut(s)	1905, 2510
CsiI	ACCWGGT	2 cut(s)	239, 1206
Csp6I	GTAC	8 cut(s)	46, 750, 1263, 1372, 1410, 1508, 1568, 2107
CspAI	ACCGGT	1 cut(s)	1193
CspCI	CAANNNNNGTGG	2 cut(s)	1908, 1943
CviQI	GTAC	8 cut(s)	46, 750, 1263, 1372, 1410, 1508, 1568, 2107
EaeI	YGGCCR	3 cut(s)	3, 1054, 1062
Eam1104I	CTCTTC	4 cut(s)	119, 973, 1862, 2060
EarI	CTCTTC	4 cut(s)	119, 973, 1862, 2060
EciI	GGCGGA	2 cut(s)	138, 1123
Ecl136II	GAGCTC	4 cut(s)	211, 1049, 1114, 1964
Eco130I	CCWWGG	1 cut(s)	1800
Eco24I	GRGCYC	5 cut(s)	213, 369, 1051, 1116, 1966
Eco31I	GGTCTC	1 cut(s)	1222
Eco47I	GGWCC	6 cut(s)	257, 820, 1104, 1191, 1210, 1973
Eco47III	AGCGCT	1 cut(s)	1202
Eco53kI	GAGCTC	4 cut(s)	211, 1049, 1114, 1964
Eco57I	CTGAAG	4 cut(s)	44, 273, 801, 1307
Eco72I	CACGTG	1 cut(s)	601
Eco81I	CCTNAGG	1 cut(s)	744
Eco88I	CYCGRG	2 cut(s)	1115, 1839
EcoICRI	GAGCTC	4 cut(s)	211, 1049, 1114, 1964
EcoNI	CCTNNNNNAGG	1 cut(s)	855
EcoO109I	RGGNCCY	1 cut(s)	1104
EcoRI	GAATTC	1 cut(s)	399
EcoRII	CCWGG	7 cut(s)	201, 239, 798, 1137, 1206, 1212, 2376
EcoT14I	CCWWGG	1 cut(s)	1800
EcoT38I	GRGCYC	5 cut(s)	213, 369, 1051, 1116, 1966
ErhI	CCWWGG	1 cut(s)	1800
Esp3I	CGTCTC	2 cut(s)	968, 1461
FaqI	GGGAC	1 cut(s)	1959
FauI	CCCGC	2 cut(s)	1838, 2416
FauNDI	CATATG	1 cut(s)	697
FokI	GGATG	3 cut(s)	1542, 1943, 2393
FriOI	GRGCYC	5 cut(s)	213, 369, 1051, 1116, 1966
FspBI	CTAG	7 cut(s)	351, 584, 765, 788, 1793, 2124, 2340
GlaI	GCGC	5 cut(s)	126, 1168, 1202, 1320, 1579
GsuI	CTGGAG	4 cut(s)	462, 821, 1971, 2386
HaeII	RGCGCY	2 cut(s)	1170, 1204
HaeIII	GGCC	7 cut(s)	5, 355, 1056, 1064, 1076, 1241, 2407
HapII	CCGG	9 cut(s)	138, 234, 1040, 1101, 1151, 1194, 1301, 1322, 2277
HgaI	GACGC	2 cut(s)	1905, 2510
HhaI	GCGC	5 cut(s)	127, 1169, 1203, 1321, 1580
Hin1I	GRCGYC	1 cut(s)	1916
Hin6I	GCGC	5 cut(s)	125, 1167, 1201, 1319, 1578
HinP1I	GCGC	5 cut(s)	125, 1167, 1201, 1319, 1578
HincII	GTYRAC	1 cut(s)	1183
HindII	GTYRAC	1 cut(s)	1183
HinfI	GANTC	9 cut(s)	154, 248, 283, 607, 1363, 1681, 1796, 2372, 2477
HpaII	CCGG	9 cut(s)	138, 234, 1040, 1101, 1151, 1194, 1301, 1322, 2277
HphI	GGTGA	1 cut(s)	1595
Hpy166II	GTNNAC	7 cut(s)	48, 752, 892, 1183, 1568, 1936, 2107
Hpy8I	GTNNAC	7 cut(s)	48, 752, 892, 1183, 1568, 1936, 2107
Hpy99I	CGWCG	4 cut(s)	193, 196, 1181, 2526
HpyAV	CCTTC	7 cut(s)	248, 310, 356, 491, 1684, 1988, 2169
HpyCH4III	ACNGT	7 cut(s)	45, 261, 646, 664, 683, 1177, 1234
HpyCH4IV	ACGT	3 cut(s)	600, 961, 993
HpySE526I	ACGT	3 cut(s)	600, 961, 993
Hsp92I	GRCGYC	1 cut(s)	1916
HspAI	GCGC	5 cut(s)	125, 1167, 1201, 1319, 1578
Kpn2I	TCCGGA	1 cut(s)	137
KspI	CCGCGG	1 cut(s)	1165
LguI	GCTCTTC	2 cut(s)	119, 2060
Lsp1109I	GCAGC	8 cut(s)	703, 1718, 1721, 1724, 1885, 1888, 2203, 2421
LweI	GCATC	8 cut(s)	112, 439, 559, 1540, 1600, 1918, 2001, 2107
MabI	ACCWGGT	2 cut(s)	239, 1206
MaeI	CTAG	7 cut(s)	351, 584, 765, 788, 1793, 2124, 2340
MaeII	ACGT	3 cut(s)	600, 961, 993
MaeIII	GTNAC	7 cut(s)	436, 502, 919, 989, 1019, 1489, 2351
MfeI	CAATTG	1 cut(s)	1386
MflI	RGATCY	5 cut(s)	337, 496, 2027, 2179, 2534
MhlI	GDGCHC	8 cut(s)	213, 369, 600, 1051, 1116, 1591, 1966, 2474
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	1 cut(s)	1790
MmeI	TCCRAC	4 cut(s)	58, 1939, 2152, 2330
Mox20I	TGGCCA	1 cut(s)	5
MroI	TCCGGA	1 cut(s)	137
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	4 cut(s)	641, 678, 2007, 2241
MslI	CAYNNNNRTG	2 cut(s)	757, 1608
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	3 cut(s)	536, 1164, 1505
MspCI	CTTAAG	1 cut(s)	2006
MspI	CCGG	9 cut(s)	138, 234, 1040, 1101, 1151, 1194, 1301, 1322, 2277
MunI	CAATTG	1 cut(s)	1386
Mva1269I	GAATGC	1 cut(s)	1498
MvaI	CCWGG	7 cut(s)	203, 241, 800, 1139, 1208, 1214, 2378
MvnI	CGCG	2 cut(s)	1164, 2103
NciI	CCSGG	3 cut(s)	1102, 1302, 2278
NdeI	CATATG	1 cut(s)	697
NlaIV	GGNNCC	8 cut(s)	238, 339, 1105, 1143, 1231, 1695, 1975, 2181
NmeAIII	GCCGAG	3 cut(s)	88, 745, 1885
NmuCI	GTSAC	4 cut(s)	436, 502, 919, 989
NspI	RCATGY	3 cut(s)	278, 1027, 1792
PaeI	GCATGC	1 cut(s)	278
PaeR7I	CTCGAG	1 cut(s)	1115
PagI	TCATGA	1 cut(s)	1546
PciI	ACATGT	1 cut(s)	1023
PciSI	GCTCTTC	2 cut(s)	119, 2060
PctI	GAATGC	1 cut(s)	1498
PfeI	GAWTC	8 cut(s)	154, 248, 283, 607, 1363, 1681, 2372, 2477
PflMI	CCANNNNNTGG	2 cut(s)	12, 829
PfoI	TCCNGGA	1 cut(s)	798
PinAI	ACCGGT	1 cut(s)	1193
PleI	GAGTC	1 cut(s)	1790
PmaCI	CACGTG	1 cut(s)	601
PmlI	CACGTG	1 cut(s)	601
PpsI	GAGTC	1 cut(s)	1790
Ppu21I	YACGTR	1 cut(s)	601
PpuMI	RGGWCCY	1 cut(s)	1104
PscI	ACATGT	1 cut(s)	1023
PsiI	TTATAA	1 cut(s)	1818
Psp124BI	GAGCTC	4 cut(s)	213, 1051, 1116, 1966
Psp5II	RGGWCCY	1 cut(s)	1104
Psp6I	CCWGG	7 cut(s)	201, 239, 798, 1137, 1206, 1212, 2376
PspCI	CACGTG	1 cut(s)	601
PspGI	CCWGG	7 cut(s)	201, 239, 798, 1137, 1206, 1212, 2376
PspN4I	GGNNCC	8 cut(s)	238, 339, 1105, 1143, 1231, 1695, 1975, 2181
PspPI	GGNCC	8 cut(s)	257, 820, 1075, 1104, 1191, 1210, 1973, 2406
PspPPI	RGGWCCY	1 cut(s)	1104
PspXI	VCTCGAGB	1 cut(s)	1115
PsrI	GAACNNNNNNTAC	2 cut(s)	1769, 1801
PstNI	CAGNNNCTG	1 cut(s)	347
PsuI	RGATCY	5 cut(s)	337, 496, 2027, 2179, 2534
PvuII	CAGCTG	2 cut(s)	536, 1505
RsaI	GTAC	8 cut(s)	47, 751, 1264, 1373, 1411, 1509, 1569, 2108
RsaNI	GTAC	8 cut(s)	46, 750, 1263, 1372, 1410, 1508, 1568, 2107
RseI	CAYNNNNRTG	2 cut(s)	757, 1608
SacI	GAGCTC	4 cut(s)	213, 1051, 1116, 1966
SacII	CCGCGG	1 cut(s)	1165
SapI	GCTCTTC	2 cut(s)	119, 2060
SaqAI	TTAA	4 cut(s)	641, 678, 2007, 2241
Sau96I	GGNCC	8 cut(s)	257, 820, 1075, 1104, 1191, 1210, 1973, 2406
ScaI	AGTACT	1 cut(s)	1411
SchI	GAGTC	1 cut(s)	1790
SduI	GDGCHC	8 cut(s)	213, 369, 600, 1051, 1116, 1591, 1966, 2474
SexAI	ACCWGGT	2 cut(s)	239, 1206
SfaNI	GCATC	8 cut(s)	112, 439, 559, 1540, 1600, 1918, 2001, 2107
Sfr274I	CTCGAG	1 cut(s)	1115
Sfr303I	CCGCGG	1 cut(s)	1165
SgrBI	CCGCGG	1 cut(s)	1165
SinI	GGWCC	6 cut(s)	257, 820, 1104, 1191, 1210, 1973
SlaI	CTCGAG	1 cut(s)	1115
SmiMI	CAYNNNNRTG	2 cut(s)	757, 1608
SmlI	CTYRAG	5 cut(s)	368, 508, 1115, 2006, 2493
SmoI	CTYRAG	5 cut(s)	368, 508, 1115, 2006, 2493
SpeI	ACTAGT	1 cut(s)	787
SphI	GCATGC	1 cut(s)	278
SspI	AATATT	1 cut(s)	2234
SspMI	CTAG	7 cut(s)	351, 584, 765, 788, 1793, 2124, 2340
SstI	GAGCTC	4 cut(s)	213, 1051, 1116, 1966
StyI	CCWWGG	1 cut(s)	1800
TaaI	ACNGT	7 cut(s)	45, 261, 646, 664, 683, 1177, 1234
TaiI	ACGT	3 cut(s)	603, 964, 996
TaqI	TCGA	6 cut(s)	82, 933, 1028, 1116, 1327, 1334
TaqII	GACCGA	1 cut(s)	808
TatI	WGTACW	3 cut(s)	1409, 1567, 2106
TauI	GCSGC	7 cut(s)	227, 1059, 1088, 1091, 1147, 1167, 2440
TfiI	GAWTC	8 cut(s)	154, 248, 283, 607, 1363, 1681, 2372, 2477
Tru1I	TTAA	4 cut(s)	641, 678, 2007, 2241
Tru9I	TTAA	4 cut(s)	641, 678, 2007, 2241
TseFI	GTSAC	4 cut(s)	436, 502, 919, 989
TseI	GCWGC	8 cut(s)	716, 1706, 1709, 1712, 1898, 1901, 2216, 2434
Tsp45I	GTSAC	4 cut(s)	436, 502, 919, 989
TspDTI	ATGAA	8 cut(s)	292, 473, 1849, 1898, 1970, 2041, 2169, 2244
TspGWI	ACGGA	4 cut(s)	977, 1693, 2324, 2476
Van91I	CCANNNNNTGG	2 cut(s)	12, 829
Vha464I	CTTAAG	1 cut(s)	2006
VpaK11BI	GGWCC	6 cut(s)	257, 820, 1104, 1191, 1210, 1973
XagI	CCTNNNNNAGG	1 cut(s)	855
XapI	RAATTY	4 cut(s)	399, 1034, 1447, 1481
XbaI	TCTAGA	1 cut(s)	583
XceI	RCATGY	3 cut(s)	278, 1027, 1792
XcmI	CCANNNNNNNNNTGG	1 cut(s)	830
XhoI	CTCGAG	1 cut(s)	1115
XspI	CTAG	7 cut(s)	351, 584, 765, 788, 1793, 2124, 2340
ZrmI	AGTACT	1 cut(s)	1411

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.