FvH4_6g43720

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002548
Physical Location & Seq
Forward (+)
33780197 .. 33783917
3721 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g43720.t1

Sequence Viewer

Length: 1824 bp
ATGAGTCTTCTGTTCTTCTCCTCCTCCTCTCCCTCAATCTTGTTCACAAAACTAACTAATACTAGTTATTTAAGAATGGCAATGCCAGCACCAGTACTGGAGCATGCACATCATACAACAGCAACAAAGCAATCCTCCTTCATTCTTTCAAACTCGTCATCCAAGGTTATTGATCCTCCGGTAGCTCGTCAACGCCGACTAGCTCAATACCATCCTACCATCTGGGGTCCCAAGCTCATCGATTCCTTCTCCACTCGCTACACTCATGAATCATATGCCTCAAAATTGGAGGGTCTGAAGCAGAATGTTTCCGAAGATTTGCATGTATTGAGTACCAAAGCTAAAGGCGATGCTTGCTTGACCTTAAAGCTCATAGACTCGATCCAGCGGTTAGGAGTATCATATCACTTCGAAAAGGAGATCAAAGCGGCTATAGTTACTCTTGTTTCTTCCAGTACTGCTAGTACCACAACAGATCTGCAAACAGTTGCTTTGCAATTTCGAATTCTAAGACAATATGGCATTTCAATTAGCTCAGATTTGTTTAACCAGTTTAGAAATTGCGAAGGAAGCTTCAAAGATAACTTAAGCAATGACGTGGAGGGACTTTTGAGTTTGTATGAGGCCTCACACCTTGGAATGCCAGGAGAAGATGTTCTAGAGGAAGCCAAGAGATTTAGTTTTAAAAACTTGAAACAATCCATGGCAACACTGAAAGACGATTATCTACTCAAGCGAGTAGCGCACTCACTGCAGACCCCTTTACACTGGAGGATGCCGAGAATAGAAGCTCTGAACTTCATTACTATGTACCAAAGGGAAGATTCTAAGAACCTGCAGGCGCTGCTGGAGTTGGCAAAATTGGACTACAATCTTGTTCAATCAGTCCATCAGATGGAGATAAAGGAGCTTTCAAGGTGGTGGAGAGAGTTGGATTTGAAATCAAAGGCATGTTTCTCAAGGGATAGACTAATGGAGAACTACATGTGGGCAATGGGGATTAGTCATGATCCCCAATTCTCGCAATGCAGAATAGGCCTCACCAAGTTTGTGTGCATATTAACAGTTATTGATGACTTGTATGATGTATACGGATTCCTGGATGAACTCGAGTGCTTTACAGATGCAGTAACTCAATGGAATATGGAAGCAAAGGAGGAACTTCCTGAGTACATGAAGCCAATCTATATTGCCATGCTCGAGTTTGGCAATGATTTGTCTGACAACATATTCAAAAACACTGGTTTAAACACCCTCCCTTATATTAAGAACGAGTGGGAAAATCTTTGCAAGTCTTATATGGTGGAAGCACGGTGGTTCTACGGCGGATACACGCCAACTCTGGAAGAGTATTTGAAGAATGCATGGACTTCAGTGGGTGGTCCGGGAGCAATGCTCCATGCTTATTTGTTAACGCAAGGATCCCAACTAACAGAAGCTTCACTTGAGTCCTTTAACCATGGCTCTCAACTGATATATTGGGCATCCTTCATAACACGACTCACCGACGATTTGGGCACTTCCAAGGCTGAGAGGGAGAGAGGCGATGTTGCGAAATCCATTCAGTGTTACATGGACGAGAAAGGCGCATCGGAAGAAGAAGCATGCGATTACATAAAGGGTTTGATCTGCCATTCATGGAAGAAAATAAACCAAGAGAGTGCCAAAACAACCATACCAAGGTCCATCGTAAACCTATCACTAAATATGGCTCGGGCTGCTCACTGCATCTTTGAACATGGGGATGGCATTGGGAACTCAATTGGGGTGACTAAGGATCGTTTGGTCTCTTTGATTGCAAATCCTATCCCCCTCGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00900 ko00902 ko00909 ko01100 ko01110 ko01130 map00900 map00902 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

608

Amino Acids

68.97

Weight (kDa)

5.91

Isoelectric Point (pI)

40.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 75 - 251 1e-48 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 309 - 549 6.7e-84 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 349 - 549 9.3e-55 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 843
AccB7I CCANNNNNTGG 1 cut(s) 895
AccI GTMKAC 1 cut(s) 1089
AciI CCGC 3 cut(s) 388, 428, 1326
AclWI GGATC 6 cut(s) 167, 376, 1004, 1416, 1429, 1785
AcsI RAATTY 1 cut(s) 504
AcuI CTGAAG 2 cut(s) 317, 1356
AfaI GTAC 6 cut(s) 96, 334, 457, 466, 812, 1172
AfiI CCNNNNNNNGG 2 cut(s) 895, 1680
AflII CTTAAG 1 cut(s) 586
AflIII ACRYGT 1 cut(s) 984
AhlI ACTAGT 1 cut(s) 62
AjiI CACGTC 1 cut(s) 598
AjnI CCWGG 2 cut(s) 643, 1098
AloI GAACNNNNNNTCC 2 cut(s) 639, 671
Alw26I GTCTC 1 cut(s) 1792
AlwI GGATC 6 cut(s) 167, 376, 1004, 1416, 1429, 1785
AlwNI CAGNNNCTG 1 cut(s) 844
Ama87I CYCGRG 3 cut(s) 1109, 1199, 1713
AoxI GGCC 2 cut(s) 624, 1036
ApeKI GCWGC 2 cut(s) 844, 1718
ApoI RAATTY 1 cut(s) 504
Asp700I GAANNNNTTC 1 cut(s) 654
AspLEI GCGC 3 cut(s) 745, 844, 1589
AspS9I GGNCC 3 cut(s) 227, 1382, 1683
AsuC2I CCSGG 1 cut(s) 1386
AsuHPI GGTGA 3 cut(s) 1033, 1495, 1780
AsuII TTCGAA 2 cut(s) 411, 502
AvaI CYCGRG 3 cut(s) 1109, 1199, 1713
AvaII GGWCC 3 cut(s) 227, 1382, 1683
BaeGI GKGCMC 1 cut(s) 1520
BamHI GGATCC 1 cut(s) 1421
BbvI GCAGC 2 cut(s) 831, 1705
BccI CCATC 6 cut(s) 219, 227, 889, 897, 1694, 1739
BceAI ACGGC 1 cut(s) 1339
BciT130I CCWGG 2 cut(s) 645, 1100
BciVI GTATCC 1 cut(s) 1322
BcnI CCSGG 1 cut(s) 1386
BcoDI GTCTC 1 cut(s) 1792
BcuI ACTAGT 1 cut(s) 62
BfaI CTAG 4 cut(s) 63, 200, 462, 659
BfmI CTRYAG 3 cut(s) 432, 752, 836
BfoI RGCGCY 1 cut(s) 845
BfrI CTTAAG 1 cut(s) 586
BfuAI ACCTGC 1 cut(s) 843
BfuI GTATCC 1 cut(s) 1322
BglII AGATCT 1 cut(s) 475
BisI GCNGC 3 cut(s) 429, 845, 1719
BlsI GCNGC 3 cut(s) 430, 846, 1720
BmcAI AGTACT 2 cut(s) 96, 457
Bme1390I CCNGG 3 cut(s) 645, 1100, 1386
Bme18I GGWCC 3 cut(s) 227, 1382, 1683
BmeT110I CYCGRG 3 cut(s) 1109, 1199, 1713
BmgBI CACGTC 1 cut(s) 598
BmgT120I GGNCC 3 cut(s) 227, 1382, 1683
BmiI GGNNCC 3 cut(s) 228, 229, 1423
BmrFI CCNGG 3 cut(s) 645, 1100, 1386
BmsI GCATC 6 cut(s) 340, 765, 1114, 1493, 1598, 1737
BplI GAGNNNNNCTC 2 cut(s) 1380, 1412
BpmI CTGGAG 3 cut(s) 119, 790, 869
Bpu14I TTCGAA 2 cut(s) 411, 502
BpuEI CTTGAG 3 cut(s) 716, 943, 1466
BpuMI CCSGG 1 cut(s) 1386
Bsa29I ATCGAT 1 cut(s) 240
BsaI GGTCTC 1 cut(s) 1792
BsaJI CCNNGG 6 cut(s) 162, 634, 702, 1459, 1524, 1679
BsaWI WCCGGW 1 cut(s) 178
BsaXI ACNNNNNCTCC 2 cut(s) 639, 669
Bsc4I CCNNNNNNNGG 2 cut(s) 895, 1680
Bse1I ACTGG 6 cut(s) 92, 102, 453, 550, 773, 1246
Bse3DI GCAATG 6 cut(s) 87, 598, 999, 1031, 1216, 1398
BseBI CCWGG 2 cut(s) 645, 1100
BseCI ATCGAT 1 cut(s) 240
BseDI CCNNGG 6 cut(s) 162, 634, 702, 1459, 1524, 1679
BseGI GGATG 6 cut(s) 158, 211, 780, 1108, 1484, 1750
BseLI CCNNNNNNNGG 2 cut(s) 895, 1680
BseMI GCAATG 6 cut(s) 87, 598, 999, 1031, 1216, 1398
BseMII CTCAG 3 cut(s) 549, 1158, 1521
BseNI ACTGG 6 cut(s) 92, 102, 453, 550, 773, 1246
BseRI GAGGAG 3 cut(s) 10, 13, 16
BseSI GKGCMC 1 cut(s) 1520
BseXI GCAGC 2 cut(s) 831, 1705
BshFI GGCC 2 cut(s) 626, 1038
BshVI ATCGAT 1 cut(s) 240
BsiHKCI CYCGRG 3 cut(s) 1109, 1199, 1713
BsiSI CCGG 2 cut(s) 179, 1385
BslFI GGGAC 2 cut(s) 213, 618
BslI CCNNNNNNNGG 2 cut(s) 895, 1680
BsmAI GTCTC 1 cut(s) 1792
BsmFI GGGAC 2 cut(s) 213, 618
BsmI GAATGC 2 cut(s) 645, 1366
BsnI GGCC 2 cut(s) 626, 1038
Bso31I GGTCTC 1 cut(s) 1792
BsoBI CYCGRG 3 cut(s) 1109, 1199, 1713
Bsp119I TTCGAA 2 cut(s) 411, 502
Bsp1286I GDGCHC 1 cut(s) 1520
Bsp143I GATC 8 cut(s) 172, 381, 420, 475, 1009, 1421, 1626, 1777
Bsp19I CCATGG 2 cut(s) 702, 1459
BspACI CCGC 3 cut(s) 388, 428, 1326
BspANI GGCC 2 cut(s) 626, 1038
BspCNI CTCAG 3 cut(s) 548, 1159, 1522
BspDI ATCGAT 1 cut(s) 240
BspHI TCATGA 2 cut(s) 265, 1006
BspLI GGNNCC 3 cut(s) 228, 229, 1423
BspMAI CTGCAG 2 cut(s) 756, 840
BspMI ACCTGC 1 cut(s) 843
BspPI GGATC 6 cut(s) 167, 376, 1004, 1416, 1429, 1785
BspT104I TTCGAA 2 cut(s) 411, 502
BspTI CTTAAG 1 cut(s) 586
BspTNI GGTCTC 1 cut(s) 1792
BsrDI GCAATG 6 cut(s) 87, 598, 999, 1031, 1216, 1398
BsrI ACTGG 6 cut(s) 92, 102, 453, 550, 773, 1246
BssECI CCNNGG 6 cut(s) 162, 634, 702, 1459, 1524, 1679
BssMI GATC 8 cut(s) 172, 381, 420, 475, 1009, 1421, 1626, 1777
BssNAI GTATAC 1 cut(s) 1090
BssT1I CCWWGG 6 cut(s) 162, 634, 702, 1459, 1524, 1679
Bst1107I GTATAC 1 cut(s) 1090
Bst2UI CCWGG 2 cut(s) 645, 1100
Bst4CI ACNGT 3 cut(s) 487, 1066, 1314
Bst6I CTCTTC 1 cut(s) 1341
BstAFI CTTAAG 1 cut(s) 586
BstAPI GCANNNNNTGC 2 cut(s) 751, 844
BstBI TTCGAA 2 cut(s) 411, 502
BstC8I GCNNGC 5 cut(s) 87, 105, 355, 840, 1606
BstDEI CTNAG 6 cut(s) 509, 535, 828, 1167, 1530, 1773
BstDSI CCRYGG 2 cut(s) 702, 1459
BstF5I GGATG 6 cut(s) 158, 211, 780, 1108, 1484, 1750
BstH2I RGCGCY 1 cut(s) 845
BstHHI GCGC 3 cut(s) 745, 844, 1589
BstKTI GATC 8 cut(s) 175, 384, 423, 478, 1012, 1424, 1629, 1780
BstMAI GTCTC 1 cut(s) 1792
BstMBI GATC 8 cut(s) 172, 381, 420, 475, 1009, 1421, 1626, 1777
BstMWI GCNNNNNNNGC 8 cut(s) 86, 354, 570, 742, 751, 844, 1035, 1718
BstNI CCWGG 2 cut(s) 645, 1100
BstNSI RCATGY 5 cut(s) 107, 326, 954, 988, 1608
BstSCI CCNGG 3 cut(s) 643, 1098, 1384
BstSFI CTRYAG 3 cut(s) 432, 752, 836
BstSLI GKGCMC 1 cut(s) 1520
BstV1I GCAGC 2 cut(s) 831, 1705
BstX2I RGATCY 2 cut(s) 475, 1421
BstYI RGATCY 2 cut(s) 475, 1421
BstZ17I GTATAC 1 cut(s) 1090
Bsu15I ATCGAT 1 cut(s) 240
BsuI GTATCC 1 cut(s) 1322
BsuRI GGCC 2 cut(s) 626, 1038
BsuTUI ATCGAT 1 cut(s) 240
BtgI CCRYGG 2 cut(s) 702, 1459
BtgZI GCGATG 2 cut(s) 363, 1560
BtrI CACGTC 1 cut(s) 598
BtsCI GGATG 6 cut(s) 158, 211, 780, 1108, 1484, 1750
BtsI GCAGTG 2 cut(s) 749, 1723
BtsIMutI CAGTG 7 cut(s) 710, 749, 766, 1239, 1380, 1571, 1723
BveI ACCTGC 1 cut(s) 843
Cac8I GCNNGC 5 cut(s) 87, 105, 355, 840, 1606
CaiI CAGNNNCTG 1 cut(s) 844
CciI TCATGA 2 cut(s) 265, 1006
CfoI GCGC 3 cut(s) 745, 844, 1589
Cfr13I GGNCC 3 cut(s) 227, 1382, 1683
ClaI ATCGAT 1 cut(s) 240
Csp6I GTAC 6 cut(s) 95, 333, 456, 465, 811, 1171
CviQI GTAC 6 cut(s) 95, 333, 456, 465, 811, 1171
DdeI CTNAG 6 cut(s) 509, 535, 828, 1167, 1530, 1773
DpnI GATC 8 cut(s) 174, 383, 422, 477, 1011, 1423, 1628, 1779
DpnII GATC 8 cut(s) 172, 381, 420, 475, 1009, 1421, 1626, 1777
DraI TTTAAA 2 cut(s) 685, 1248
Eam1104I CTCTTC 1 cut(s) 1341
EarI CTCTTC 1 cut(s) 1341
EciI GGCGGA 1 cut(s) 1341
Eco130I CCWWGG 6 cut(s) 162, 634, 702, 1459, 1524, 1679
Eco147I AGGCCT 2 cut(s) 626, 1038
Eco31I GGTCTC 1 cut(s) 1792
Eco47I GGWCC 3 cut(s) 227, 1382, 1683
Eco57I CTGAAG 2 cut(s) 317, 1356
Eco88I CYCGRG 3 cut(s) 1109, 1199, 1713
EcoO109I RGGNCCY 1 cut(s) 227
EcoRI GAATTC 1 cut(s) 504
EcoRII CCWGG 2 cut(s) 643, 1098
EcoT14I CCWWGG 6 cut(s) 162, 634, 702, 1459, 1524, 1679
EcoT22I ATGCAT 1 cut(s) 1366
ErhI CCWWGG 6 cut(s) 162, 634, 702, 1459, 1524, 1679
FalI AAGNNNNNCTT 2 cut(s) 1428, 1460
FaqI GGGAC 2 cut(s) 213, 618
FauNDI CATATG 1 cut(s) 274
FblI GTMKAC 1 cut(s) 1089
Fnu4HI GCNGC 3 cut(s) 429, 845, 1719
FokI GGATG 6 cut(s) 145, 198, 787, 1115, 1471, 1757
Fsp4HI GCNGC 3 cut(s) 429, 845, 1719
FspBI CTAG 4 cut(s) 63, 200, 462, 659
GlaI GCGC 3 cut(s) 744, 843, 1588
GluI GCNGC 3 cut(s) 429, 845, 1719
GsuI CTGGAG 3 cut(s) 119, 790, 869
HaeII RGCGCY 1 cut(s) 845
HaeIII GGCC 2 cut(s) 626, 1038
HapII CCGG 2 cut(s) 179, 1385
HhaI GCGC 3 cut(s) 745, 844, 1589
Hin6I GCGC 3 cut(s) 743, 842, 1587
HinP1I GCGC 3 cut(s) 743, 842, 1587
HincII GTYRAC 2 cut(s) 191, 1413
HindII GTYRAC 2 cut(s) 191, 1413
HindIII AAGCTT 2 cut(s) 571, 1437
HinfI GANTC 8 cut(s) 4, 242, 269, 377, 824, 1095, 1448, 1500
HpaI GTTAAC 1 cut(s) 1413
HpaII CCGG 2 cut(s) 179, 1385
HphI GGTGA 3 cut(s) 1033, 1495, 1780
Hpy166II GTNNAC 5 cut(s) 45, 191, 1090, 1413, 1693
Hpy188I TCNGA 7 cut(s) 297, 313, 538, 795, 894, 1222, 1594
Hpy188III TCNNGA 5 cut(s) 266, 659, 1007, 1166, 1343
Hpy8I GTNNAC 5 cut(s) 45, 191, 1090, 1413, 1693
Hpy99I CGWCG 1 cut(s) 1511
HpyAV CCTTC 4 cut(s) 148, 256, 560, 1498
HpyCH4III ACNGT 3 cut(s) 487, 1066, 1314
HpyCH4IV ACGT 1 cut(s) 597
HpyF10VI GCNNNNNNNGC 8 cut(s) 86, 354, 570, 742, 751, 844, 1035, 1718
HpyF3I CTNAG 6 cut(s) 509, 535, 828, 1167, 1530, 1773
HpySE526I ACGT 1 cut(s) 597
HspAI GCGC 3 cut(s) 743, 842, 1587
KflI GGGWCCC 1 cut(s) 227
KspAI GTTAAC 1 cut(s) 1413
Kzo9I GATC 8 cut(s) 172, 381, 420, 475, 1009, 1421, 1626, 1777
LmnI GCTCC 4 cut(s) 100, 907, 1388, 1401
Lsp1109I GCAGC 2 cut(s) 831, 1705
LweI GCATC 6 cut(s) 340, 765, 1114, 1493, 1598, 1737
MaeI CTAG 4 cut(s) 63, 200, 462, 659
MaeII ACGT 1 cut(s) 597
MaeIII GTNAC 4 cut(s) 436, 1129, 1568, 1768
MalI GATC 8 cut(s) 174, 383, 422, 477, 1011, 1423, 1628, 1779
MboI GATC 8 cut(s) 172, 381, 420, 475, 1009, 1421, 1626, 1777
MfeI CAATTG 1 cut(s) 1761
MflI RGATCY 2 cut(s) 475, 1421
MhlI GDGCHC 1 cut(s) 1520
MluCI AATT 8 cut(s) 284, 497, 504, 528, 559, 860, 1016, 1761
MlyI GAGTC 4 cut(s) 13, 371, 1457, 1494
MmeI TCCRAC 1 cut(s) 912
Mph1103I ATGCAT 1 cut(s) 1366
MroXI GAANNNNTTC 1 cut(s) 654
MslI CAYNNNNRTG 2 cut(s) 806, 1743
MspA1I CMGCKG 1 cut(s) 388
MspCI CTTAAG 1 cut(s) 586
MspI CCGG 2 cut(s) 179, 1385
MspR9I CCNGG 3 cut(s) 645, 1100, 1386
MssI GTTTAAAC 1 cut(s) 1248
MunI CAATTG 1 cut(s) 1761
Mva1269I GAATGC 2 cut(s) 645, 1366
MvaI CCWGG 2 cut(s) 645, 1100
MwoI GCNNNNNNNGC 8 cut(s) 86, 354, 570, 742, 751, 844, 1035, 1718
NciI CCSGG 1 cut(s) 1386
NcoI CCATGG 2 cut(s) 702, 1459
NdeI CATATG 1 cut(s) 274
NdeII GATC 8 cut(s) 172, 381, 420, 475, 1009, 1421, 1626, 1777
NlaIV GGNNCC 3 cut(s) 228, 229, 1423
NmeAIII GCCGAG 1 cut(s) 804
NmuCI GTSAC 1 cut(s) 1768
NsiI ATGCAT 1 cut(s) 1366
NspI RCATGY 5 cut(s) 107, 326, 954, 988, 1608
NspV TTCGAA 2 cut(s) 411, 502
PaeI GCATGC 2 cut(s) 107, 1608
PaeR7I CTCGAG 2 cut(s) 1109, 1199
PagI TCATGA 2 cut(s) 265, 1006
PceI AGGCCT 2 cut(s) 626, 1038
PciI ACATGT 1 cut(s) 984
PcsI WCGNNNNNNNCGW 1 cut(s) 193
PctI GAATGC 2 cut(s) 645, 1366
PdmI GAANNNNTTC 1 cut(s) 654
PfeI GAWTC 4 cut(s) 242, 269, 824, 1095
PflMI CCANNNNNTGG 1 cut(s) 895
PfoI TCCNGGA 2 cut(s) 1098, 1384
PkrI GCNGC 3 cut(s) 430, 846, 1720
PleI GAGTC 4 cut(s) 12, 371, 1456, 1494
PmeI GTTTAAAC 1 cut(s) 1248
PpsI GAGTC 4 cut(s) 12, 371, 1456, 1494
PpuMI RGGWCCY 1 cut(s) 227
PscI ACATGT 1 cut(s) 984
Psp5II RGGWCCY 1 cut(s) 227
Psp6I CCWGG 2 cut(s) 643, 1098
PspGI CCWGG 2 cut(s) 643, 1098
PspN4I GGNNCC 3 cut(s) 228, 229, 1423
PspPI GGNCC 3 cut(s) 227, 1382, 1683
PspPPI RGGWCCY 1 cut(s) 227
PspXI VCTCGAGB 2 cut(s) 1109, 1199
PstI CTGCAG 2 cut(s) 756, 840
PstNI CAGNNNCTG 1 cut(s) 844
PsuI RGATCY 2 cut(s) 475, 1421
RsaI GTAC 6 cut(s) 96, 334, 457, 466, 812, 1172
RsaNI GTAC 6 cut(s) 95, 333, 456, 465, 811, 1171
RseI CAYNNNNRTG 2 cut(s) 806, 1743
SatI GCNGC 3 cut(s) 429, 845, 1719
Sau3AI GATC 8 cut(s) 172, 381, 420, 475, 1009, 1421, 1626, 1777
Sau96I GGNCC 3 cut(s) 227, 1382, 1683
SbfI CCTGCAGG 1 cut(s) 840
ScaI AGTACT 2 cut(s) 96, 457
SchI GAGTC 4 cut(s) 13, 371, 1457, 1494
ScrFI CCNGG 3 cut(s) 645, 1100, 1386
SdaI CCTGCAGG 1 cut(s) 840
SduI GDGCHC 1 cut(s) 1520
SfaNI GCATC 6 cut(s) 340, 765, 1114, 1493, 1598, 1737
SfcI CTRYAG 3 cut(s) 432, 752, 836
Sfr274I CTCGAG 2 cut(s) 1109, 1199
SfuI TTCGAA 2 cut(s) 411, 502
SinI GGWCC 3 cut(s) 227, 1382, 1683
SlaI CTCGAG 2 cut(s) 1109, 1199
SmiMI CAYNNNNRTG 2 cut(s) 806, 1743
SmlI CTYRAG 6 cut(s) 586, 731, 958, 1109, 1199, 1445
SmoI CTYRAG 6 cut(s) 586, 731, 958, 1109, 1199, 1445
SpeI ACTAGT 1 cut(s) 62
SphI GCATGC 2 cut(s) 107, 1608
Sse8387I CCTGCAGG 1 cut(s) 840
Sse9I AATT 8 cut(s) 284, 497, 504, 528, 559, 860, 1016, 1761
SseBI AGGCCT 2 cut(s) 626, 1038
SsiI CCGC 3 cut(s) 388, 428, 1326
SspMI CTAG 4 cut(s) 63, 200, 462, 659
StuI AGGCCT 2 cut(s) 626, 1038
StyD4I CCNGG 3 cut(s) 643, 1098, 1384
StyI CCWWGG 6 cut(s) 162, 634, 702, 1459, 1524, 1679
TaaI ACNGT 3 cut(s) 487, 1066, 1314
TaiI ACGT 1 cut(s) 600
TaqI TCGA 7 cut(s) 240, 380, 411, 502, 1110, 1200, 1815
TasI AATT 8 cut(s) 284, 497, 504, 528, 559, 860, 1016, 1761
TatI WGTACW 3 cut(s) 94, 455, 1170
TauI GCSGC 1 cut(s) 431
TfiI GAWTC 4 cut(s) 242, 269, 824, 1095
TscAI CASTG 7 cut(s) 717, 756, 773, 1246, 1380, 1571, 1730
TseFI GTSAC 1 cut(s) 1768
TseI GCWGC 2 cut(s) 844, 1718
Tsp45I GTSAC 1 cut(s) 1768
TspDTI ATGAA 7 cut(s) 130, 282, 790, 1119, 1190, 1480, 1626
TspGWI ACGGA 1 cut(s) 1107
TspRI CASTG 7 cut(s) 717, 756, 773, 1246, 1380, 1571, 1730
Van91I CCANNNNNTGG 1 cut(s) 895
Vha464I CTTAAG 1 cut(s) 586
VpaK11BI GGWCC 3 cut(s) 227, 1382, 1683
XapI RAATTY 1 cut(s) 504
XbaI TCTAGA 1 cut(s) 658
XceI RCATGY 5 cut(s) 107, 326, 954, 988, 1608
XhoI CTCGAG 2 cut(s) 1109, 1199
XmiI GTMKAC 1 cut(s) 1089
XmnI GAANNNNTTC 1 cut(s) 654
XspI CTAG 4 cut(s) 63, 200, 462, 659
ZrmI AGTACT 2 cut(s) 96, 457
Zsp2I ATGCAT 1 cut(s) 1366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.