MD17G1091300.v1.1

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002548
Physical Location & Seq
Reverse (-)
7617046 .. 7620576
3531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1091300.v1.1.491

Sequence Viewer

Length: 1839 bp
ATGGCACTTACAACATTCTCCTCGCTCTCTCCCTCTTTGGTCTCAACTAGTACTAGGTATCAAAGATTAATCCATCTTCCAAAAGTTCTGCTTCAAAGAACAGTAGGAAAACAACCCTCCTTCCTTTATGTATCAATAAACTCATCACCGTCATCGAAGGTTATCAGTAATCCTCCGGCAGTGCAACGGCGATTCGCTCAATACCATCCGACCATTTGGGATTCCAAACTCATCGACACATTCACCACTCATTACACGTATGAATTGCATGGGACGCGATTGGATCGTCTAAAGCAGAACGTTACTGAAACGTTGCTGGCAGCATCAACTAGTAGTACTCAAGGCACTACTAGCACTTTATCGATGTTAAAGCTTATCGACTCAATGCAGCGATTGGGAGTGGCCTATCATTTCGAAAAAGAAATCGAAGCAGTTTTAACTAGCCTTGGTTCTTCCAGTACTGCCAGTACATCAAGTGATCTTCACACCGTTGCCTTGCAGTTTCGGATATTACGAGAACATGGTATCCCTATTAGCCCAGATGTGTTTAACAAGTTTAAAGCCGGAAACGGAAGATTTGAGGATAGCTTAAGCAAGGACATTGAAGGACTTTTGAGCTTGTATGAAGCCTCACACCTTGGTATGCTTGGGGAAGAGCATGTTTTGGAGGAAGCCAAGAGTTTTAGTGCCAAAAGGTTGAGACAATTATTGGGAACATTGGAGGGTGAAGATGGTTTATTGAAGCAACTAGTTCAGCAATCAATGGAAACCCCCTTACATTGGAGGATGCCGAGGATAGAAGCTCGAAGTTATATTGATTTATACCAAAAGGACGATTCTAGGAACATCGATTTGCTTGAATTAGCCAAATTGGATTATAATCTTGTCCAATCAGTATATCAGAGGGAGATAAAGGAGCTATCAAGGTGGTGGACGGATTTGGATTTTGAACATAAGGCAAATTTTTCGAGAGACAGATTGATGGAGAACTATTTGTGGGCAATGGGAATCAATTATGAGCCCCAATTCTCGAATTGCAGGATTGGCCTCACCAAATTCGTTTGCATATTAACAGTAATTGATGACATGTACGATGTTTATGGATTTTTGGATGAACTTGAGCTGTTTACAGATGCAGTACATAGGTGGAATATGGAGGACACGGAGGAGCTTCCCGAGTACATGAAGCCAATTTATACAGCCTTGCTCAACTTTGGGAATGAAGTAAATGATAACGTATTTAAAAACAACGGTTTAAGCGTCCTCCCCTATATTAAGAAAGAGTGGGTAAATCTTTGTAATTCATATCTGGTAGAGGCACGATGGTTTTACTGTGGATACACACCAACCCTAGAAGAGTACTTAAAGAATGCATGGACTTCAGTGGGTGGTCCTGAGGCAATTCTCCATGCTTATTTGTTGTTGCAAGGACGCAACATAACAAAAAGCTCACTTGACTCCTTTAAGCATGGCTCTCAAATAATATATTGGTCATCTCTAATGACTCGGCTTAGTGATGATTTGGCCACTTCCAAGGCTGAGGGTGAGAGAGGTGATGTGGCAAAATCTGTTGAGTGCTGCATGAAAGAAAGAGGCGTATCTGAAGAAGAAGCGCAGGACTACATAAACGAATTGATCTGCTATTCGTGGACGAAGATTAATGAGGAGAGTTCAAAAACTGCCATACCAAAATCAATTGTAAAACTGTCACTGAATATGGCTCGGACTGCTCACTCTATTTTCCAACATGGAGACGGTATTGGAACTTCAATTGGGGTCACCAAAGATCGTTTAATCTCTTTAATTTCTAATCCTATCCCTATCAACACTCACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00900 ko00902 ko00909 ko01100 ko01110 ko01130 map00900 map00902 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

613

Amino Acids

69.69

Weight (kDa)

5.78

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 73 - 252 4.2e-46 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 313 - 551 4.5e-87 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 352 - 551 2.6e-55 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 879
AccII CGCG 1 cut(s) 277
AclI AACGTT 2 cut(s) 300, 311
AclWI GGATC 1 cut(s) 291
AcoI YGGCCR 1 cut(s) 1524
AcsI RAATTY 2 cut(s) 961, 1055
AcuI CTGAAG 2 cut(s) 1365, 1623
AfaI GTAC 8 cut(s) 52, 337, 460, 469, 1091, 1140, 1181, 1361
AfiI CCNNNNNNNGG 1 cut(s) 780
AflII CTTAAG 1 cut(s) 589
AflIII ACRYGT 2 cut(s) 255, 1086
AgsI TTSAA 7 cut(s) 95, 605, 742, 860, 950, 1674, 1770
AhlI ACTAGT 3 cut(s) 47, 329, 748
AloI GAACNNNNNNTCC 2 cut(s) 510, 542
AluBI AGCT 8 cut(s) 373, 588, 618, 803, 919, 1123, 1171, 1449
AluI AGCT 8 cut(s) 373, 588, 618, 803, 919, 1123, 1171, 1449
Alw26I GTCTC 4 cut(s) 46, 694, 966, 1746
AlwI GGATC 1 cut(s) 291
Ama87I CYCGRG 1 cut(s) 1175
AoxI GGCC 3 cut(s) 402, 1045, 1524
ApeKI GCWGC 3 cut(s) 320, 388, 1578
ApoI RAATTY 2 cut(s) 961, 1055
AseI ATTAAT 2 cut(s) 68, 1659
AspLEI GCGC 1 cut(s) 1615
AspS9I GGNCC 1 cut(s) 1391
AsuHPI GGTGA 7 cut(s) 138, 235, 737, 1042, 1556, 1565, 1771
AsuII TTCGAA 1 cut(s) 414
AvaI CYCGRG 1 cut(s) 1175
AvaII GGWCC 1 cut(s) 1391
AxyI CCTNAGG 1 cut(s) 1395
BalI TGGCCA 1 cut(s) 1526
BanII GRGCYC 1 cut(s) 1023
BarI GAAGNNNNNNTAC 2 cut(s) 1750, 1782
BbvCI CCTCAGC 1 cut(s) 1539
BbvI GCAGC 3 cut(s) 332, 400, 1565
BccI CCATC 5 cut(s) 81, 213, 725, 976, 1317
BceAI ACGGC 1 cut(s) 203
BcgI CGANNNNNNTGC 2 cut(s) 948, 982
BciVI GTATCC 2 cut(s) 536, 1331
BcoDI GTCTC 4 cut(s) 46, 694, 966, 1746
BcuI ACTAGT 3 cut(s) 47, 329, 748
BfaI CTAG 8 cut(s) 48, 54, 330, 351, 441, 749, 840, 1352
BfrI CTTAAG 1 cut(s) 589
BfuI GTATCC 2 cut(s) 536, 1331
BisI GCNGC 3 cut(s) 321, 389, 1579
BlsI GCNGC 3 cut(s) 322, 390, 1580
BmcAI AGTACT 4 cut(s) 52, 337, 460, 1361
Bme18I GGWCC 1 cut(s) 1391
BmeT110I CYCGRG 1 cut(s) 1175
BmgT120I GGNCC 1 cut(s) 1391
BmsI GCATC 3 cut(s) 332, 777, 1123
Bpu10I CCTNAGC 1 cut(s) 1539
Bpu14I TTCGAA 1 cut(s) 414
BpuEI CTTGAG 2 cut(s) 324, 1139
Bsa29I ATCGAT 2 cut(s) 362, 849
BsaAI YACGTR 1 cut(s) 258
BsaBI GATNNNNATC 1 cut(s) 879
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 4 cut(s) 445, 637, 791, 1533
Bsc4I CCNNNNNNNGG 1 cut(s) 780
Bse1I ACTGG 2 cut(s) 456, 465
Bse21I CCTNAGG 1 cut(s) 1395
Bse3DI GCAATG 1 cut(s) 1008
Bse8I GATNNNNATC 1 cut(s) 879
BseCI ATCGAT 2 cut(s) 362, 849
BseDI CCNNGG 4 cut(s) 445, 637, 791, 1533
BseGI GGATG 3 cut(s) 205, 792, 1117
BseJI GATNNNNATC 1 cut(s) 879
BseLI CCNNNNNNNGG 1 cut(s) 780
BseMI GCAATG 1 cut(s) 1008
BseMII CTCAG 2 cut(s) 1386, 1530
BseNI ACTGG 2 cut(s) 456, 465
BseRI GAGGAG 3 cut(s) 10, 1181, 1679
BseXI GCAGC 3 cut(s) 332, 400, 1565
Bsh1236I CGCG 1 cut(s) 277
BshFI GGCC 3 cut(s) 404, 1047, 1526
BshVI ATCGAT 2 cut(s) 362, 849
BsiHKCI CYCGRG 1 cut(s) 1175
BsiSI CCGG 2 cut(s) 176, 564
BslFI GGGAC 1 cut(s) 286
BslI CCNNNNNNNGG 1 cut(s) 780
BsmAI GTCTC 4 cut(s) 46, 694, 966, 1746
BsmBI CGTCTC 1 cut(s) 1746
BsmFI GGGAC 1 cut(s) 286
BsmI GAATGC 1 cut(s) 1375
BsnI GGCC 3 cut(s) 404, 1047, 1526
Bso31I GGTCTC 1 cut(s) 46
BsoBI CYCGRG 1 cut(s) 1175
Bsp119I TTCGAA 1 cut(s) 414
Bsp1286I GDGCHC 1 cut(s) 1023
Bsp143I GATC 4 cut(s) 283, 478, 1635, 1786
BspANI GGCC 3 cut(s) 404, 1047, 1526
BspCNI CTCAG 2 cut(s) 1387, 1531
BspDI ATCGAT 2 cut(s) 362, 849
BspFNI CGCG 1 cut(s) 277
BspPI GGATC 1 cut(s) 291
BspQI GCTCTTC 1 cut(s) 648
BspT104I TTCGAA 1 cut(s) 414
BspTI CTTAAG 1 cut(s) 589
BspTNI GGTCTC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 1008
BsrI ACTGG 2 cut(s) 456, 465
BssECI CCNNGG 4 cut(s) 445, 637, 791, 1533
BssMI GATC 4 cut(s) 283, 478, 1635, 1786
BssT1I CCWWGG 3 cut(s) 445, 637, 1533
Bst4CI ACNGT 8 cut(s) 103, 150, 490, 1075, 1253, 1334, 1707, 1757
Bst6I CTCTTC 2 cut(s) 648, 1350
BstAFI CTTAAG 1 cut(s) 589
BstBAI YACGTR 1 cut(s) 258
BstBI TTCGAA 1 cut(s) 414
BstC8I GCNNGC 1 cut(s) 318
BstDEI CTNAG 3 cut(s) 1395, 1511, 1539
BstEII GGTNACC 1 cut(s) 1777
BstF5I GGATG 3 cut(s) 205, 792, 1117
BstFNI CGCG 1 cut(s) 277
BstHHI GCGC 1 cut(s) 1615
BstKTI GATC 4 cut(s) 286, 481, 1638, 1789
BstMAI GTCTC 4 cut(s) 46, 694, 966, 1746
BstMBI GATC 4 cut(s) 283, 478, 1635, 1786
BstMWI GCNNNNNNNGC 4 cut(s) 274, 351, 1044, 1727
BstNSI RCATGY 2 cut(s) 662, 1090
BstPI GGTNACC 1 cut(s) 1777
BstUI CGCG 1 cut(s) 277
BstV1I GCAGC 3 cut(s) 332, 400, 1565
Bsu15I ATCGAT 2 cut(s) 362, 849
Bsu36I CCTNAGG 1 cut(s) 1395
BsuI GTATCC 2 cut(s) 536, 1331
BsuRI GGCC 3 cut(s) 404, 1047, 1526
BsuTUI ATCGAT 2 cut(s) 362, 849
BtsCI GGATG 3 cut(s) 205, 792, 1117
BtsI GCAGTG 1 cut(s) 186
BtsIMutI CAGTG 3 cut(s) 186, 1389, 1709
Cac8I GCNNGC 1 cut(s) 318
CfoI GCGC 1 cut(s) 1615
Cfr13I GGNCC 1 cut(s) 1391
ClaI ATCGAT 2 cut(s) 362, 849
CseI GACGC 3 cut(s) 283, 1249, 1440
Csp6I GTAC 8 cut(s) 51, 336, 459, 468, 1090, 1139, 1180, 1360
CviQI GTAC 8 cut(s) 51, 336, 459, 468, 1090, 1139, 1180, 1360
DdeI CTNAG 3 cut(s) 1395, 1511, 1539
DpnI GATC 4 cut(s) 285, 480, 1637, 1788
DpnII GATC 4 cut(s) 283, 478, 1635, 1786
DraI TTTAAA 2 cut(s) 559, 1243
EaeI YGGCCR 1 cut(s) 1524
Eam1104I CTCTTC 2 cut(s) 648, 1350
EarI CTCTTC 2 cut(s) 648, 1350
Eco130I CCWWGG 3 cut(s) 445, 637, 1533
Eco24I GRGCYC 1 cut(s) 1023
Eco31I GGTCTC 1 cut(s) 46
Eco47I GGWCC 1 cut(s) 1391
Eco57I CTGAAG 2 cut(s) 1365, 1623
Eco81I CCTNAGG 1 cut(s) 1395
Eco88I CYCGRG 1 cut(s) 1175
Eco91I GGTNACC 1 cut(s) 1777
EcoO65I GGTNACC 1 cut(s) 1777
EcoT14I CCWWGG 3 cut(s) 445, 637, 1533
EcoT22I ATGCAT 1 cut(s) 1375
EcoT38I GRGCYC 1 cut(s) 1023
ErhI CCWWGG 3 cut(s) 445, 637, 1533
Esp3I CGTCTC 1 cut(s) 1746
FalI AAGNNNNNCTT 2 cut(s) 75, 107
FaqI GGGAC 1 cut(s) 286
Fnu4HI GCNGC 3 cut(s) 321, 389, 1579
FokI GGATG 3 cut(s) 192, 799, 1124
FriOI GRGCYC 1 cut(s) 1023
Fsp4HI GCNGC 3 cut(s) 321, 389, 1579
FspBI CTAG 8 cut(s) 48, 54, 330, 351, 441, 749, 840, 1352
GlaI GCGC 1 cut(s) 1614
GluI GCNGC 3 cut(s) 321, 389, 1579
HaeIII GGCC 3 cut(s) 404, 1047, 1526
HapII CCGG 2 cut(s) 176, 564
HgaI GACGC 3 cut(s) 283, 1249, 1440
HhaI GCGC 1 cut(s) 1615
Hin6I GCGC 1 cut(s) 1613
HinP1I GCGC 1 cut(s) 1613
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 7 cut(s) 192, 221, 380, 836, 1008, 1457, 1504
HpaII CCGG 2 cut(s) 176, 564
HphI GGTGA 7 cut(s) 138, 235, 737, 1042, 1556, 1565, 1771
Hpy166II GTNNAC 3 cut(s) 933, 1128, 1650
Hpy188I TCNGA 5 cut(s) 210, 507, 903, 1603, 1725
Hpy188III TCNNGA 4 cut(s) 969, 1030, 1175, 1394
Hpy8I GTNNAC 3 cut(s) 933, 1128, 1650
HpyAV CCTTC 3 cut(s) 130, 151, 599
HpyCH4III ACNGT 8 cut(s) 103, 150, 490, 1075, 1253, 1334, 1707, 1757
HpyCH4IV ACGT 4 cut(s) 257, 300, 311, 1236
HpyF10VI GCNNNNNNNGC 4 cut(s) 274, 351, 1044, 1727
HpyF3I CTNAG 3 cut(s) 1395, 1511, 1539
HpySE526I ACGT 4 cut(s) 257, 300, 311, 1236
HspAI GCGC 1 cut(s) 1613
Kzo9I GATC 4 cut(s) 283, 478, 1635, 1786
LguI GCTCTTC 1 cut(s) 648
LmnI GCTCC 2 cut(s) 916, 1168
Lsp1109I GCAGC 3 cut(s) 332, 400, 1565
LweI GCATC 3 cut(s) 332, 777, 1123
MaeI CTAG 8 cut(s) 48, 54, 330, 351, 441, 749, 840, 1352
MaeII ACGT 4 cut(s) 257, 300, 311, 1236
MaeIII GTNAC 3 cut(s) 301, 1707, 1777
MalI GATC 4 cut(s) 285, 480, 1637, 1788
MboI GATC 4 cut(s) 283, 478, 1635, 1786
MfeI CAATTG 2 cut(s) 1695, 1770
MhlI GDGCHC 1 cut(s) 1023
MlsI TGGCCA 1 cut(s) 1526
MluNI TGGCCA 1 cut(s) 1526
MlyI GAGTC 3 cut(s) 374, 1451, 1498
MmeI TCCRAC 2 cut(s) 233, 1768
Mox20I TGGCCA 1 cut(s) 1526
Mph1103I ATGCAT 1 cut(s) 1375
MscI TGGCCA 1 cut(s) 1526
Msp20I TGGCCA 1 cut(s) 1526
MspCI CTTAAG 1 cut(s) 589
MspI CCGG 2 cut(s) 176, 564
MunI CAATTG 2 cut(s) 1695, 1770
Mva1269I GAATGC 1 cut(s) 1375
MvnI CGCG 1 cut(s) 277
MwoI GCNNNNNNNGC 4 cut(s) 274, 351, 1044, 1727
NdeII GATC 4 cut(s) 283, 478, 1635, 1786
NmeAIII GCCGAG 2 cut(s) 816, 1486
NmuCI GTSAC 2 cut(s) 1707, 1777
NsiI ATGCAT 1 cut(s) 1375
NspI RCATGY 2 cut(s) 662, 1090
NspV TTCGAA 1 cut(s) 414
PciI ACATGT 1 cut(s) 1086
PciSI GCTCTTC 1 cut(s) 648
PcsI WCGNNNNNNNCGW 2 cut(s) 511, 1257
PctI GAATGC 1 cut(s) 1375
PfeI GAWTC 4 cut(s) 192, 221, 836, 1008
PkrI GCNGC 3 cut(s) 322, 390, 1580
PleI GAGTC 3 cut(s) 374, 1451, 1498
PpsI GAGTC 3 cut(s) 374, 1451, 1498
Ppu21I YACGTR 1 cut(s) 258
PscI ACATGT 1 cut(s) 1086
PshBI ATTAAT 2 cut(s) 68, 1659
PsiI TTATAA 1 cut(s) 879
Psp1406I AACGTT 2 cut(s) 300, 311
PspEI GGTNACC 1 cut(s) 1777
PspPI GGNCC 1 cut(s) 1391
RsaI GTAC 8 cut(s) 52, 337, 460, 469, 1091, 1140, 1181, 1361
RsaNI GTAC 8 cut(s) 51, 336, 459, 468, 1090, 1139, 1180, 1360
SapI GCTCTTC 1 cut(s) 648
SatI GCNGC 3 cut(s) 321, 389, 1579
Sau3AI GATC 4 cut(s) 283, 478, 1635, 1786
Sau96I GGNCC 1 cut(s) 1391
ScaI AGTACT 4 cut(s) 52, 337, 460, 1361
SchI GAGTC 3 cut(s) 374, 1451, 1498
SduI GDGCHC 1 cut(s) 1023
SfaNI GCATC 3 cut(s) 332, 777, 1123
SfuI TTCGAA 1 cut(s) 414
SinI GGWCC 1 cut(s) 1391
SmlI CTYRAG 3 cut(s) 339, 589, 1118
SmoI CTYRAG 3 cut(s) 339, 589, 1118
SpeI ACTAGT 3 cut(s) 47, 329, 748
SspMI CTAG 8 cut(s) 48, 54, 330, 351, 441, 749, 840, 1352
StyI CCWWGG 3 cut(s) 445, 637, 1533
TaaI ACNGT 8 cut(s) 103, 150, 490, 1075, 1253, 1334, 1707, 1757
TaiI ACGT 4 cut(s) 260, 303, 314, 1239
TatI WGTACW 7 cut(s) 50, 335, 458, 467, 1138, 1179, 1359
TfiI GAWTC 4 cut(s) 192, 221, 836, 1008
TscAI CASTG 3 cut(s) 186, 1389, 1716
TseFI GTSAC 2 cut(s) 1707, 1777
TseI GCWGC 3 cut(s) 320, 388, 1578
Tsp45I GTSAC 2 cut(s) 1707, 1777
TspDTI ATGAA 7 cut(s) 276, 639, 1128, 1199, 1236, 1293, 1598
TspGWI ACGGA 3 cut(s) 585, 950, 1178
TspRI CASTG 3 cut(s) 186, 1389, 1716
Vha464I CTTAAG 1 cut(s) 589
VpaK11BI GGWCC 1 cut(s) 1391
VspI ATTAAT 2 cut(s) 68, 1659
XapI RAATTY 2 cut(s) 961, 1055
XceI RCATGY 2 cut(s) 662, 1090
XspI CTAG 8 cut(s) 48, 54, 330, 351, 441, 749, 840, 1352
ZrmI AGTACT 4 cut(s) 52, 337, 460, 1361
Zsp2I ATGCAT 1 cut(s) 1375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.