Rw2G045400

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002548
Physical Location & Seq
Forward (+)
72319091 .. 72322334
3244 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G045400.1

Sequence Viewer

Length: 1809 bp
ATGAATCTTGTATTCTCCTCCTCCTCCTCTCCCTCAGTCTTGCTCACAAAGCTCACAAGAACTAGTCATCCAAGAATGCTAGTTCCAGCACTGCTAGTACAAGCGTATCAGACAAGAAGAGGACAAGCCATTGTCTCAAACTCATCATCATCAAAGGTCATTGATCATCCTCCTGCAGCTCAACGCCGATTAGCTCAATACCATCCTAGCATTTGGGGTCCCGGCCTCATCGATTCCTTCACCACTCACTACACTTATGAATCACATGCCACACGGTGGGAGGGTCTAAAGCAGAACGTAGCCAAAGCCTTAGTCGCATCCAGTACTAAAGACGATGCTTGCTCCATTCTGAAGCTTATAGCCTCGGTGCAGCGGTTGGGAGTAGCATACCACTTCCGAAAGGAGATCCAAGCAGCTCTGGTTACTCTGATTTCTTCCACTAGTACCCTTAGTACCACAAATGATCTTCATACAGCAGCCTTGCAATTTCAAATACTAAGAGAAAATGGTATATCAGTAAGCTCAGATTTGTTTAACAAGTTTAGAAGTGGAGAAGGAAGCTTCAAAGATAGCTTAAGCAACGGTGTGGAGGGACTTTTGAGTTTGAATGAAGCCTCACACCTTGGAATGCCAGGAGAAGATGTTCTAGAGGAAGCTAAGAGATTTAGTTCAAAAAATTTGAAACAATCCATGTCAACAATATTGAACGACGATAATCTACTGAAGCAAGTAGAGCAATCACTGCAAACCCCTGTGCACTGGAGGATGCCTAGGATAGAAGCTCTGAACTTCATCGATATCTACCAAAGGGGCGACTCTAAGAACCTGGCACTGCTGGAGTTGGCTAAATTGGACTATAATCTTGTTCAATCAGTATATCAAATTGAGATAAAAGAGCTCTCTAGGTGGTGGAGAGAGTTGGATTTTAAATCGAAGGCAAGTTTCTCAAGGGACAGACTGATGGAGAACTACTTGTGGTCAATGGGGATTTGTCACGAGCCCCAATTCTCAGAATGCAGGATAGGCCTCACCAAGTTTGTGTGCATATTAACAGTCATTGATGACATGTATGATGTATATGGATTCCTGGATGAGCTTGAGTGCTTTACAGATGCAGTAATTCAATGGAATATGGAAGCAAAGGAGGAACTTCCCGAGTACATGAAGCCAATCTATATTGCCATGCTCGAGTTTGGCAATGAATTGGCTGATAATATATTCACAAATAATGGTTTAAACACCCTCCCTTATATTAAGAACGAGTGGGTTAATCTTTGTACAGCCTATATGGTGGAAGCACGGTGGTTCTACGGCCGATTCACACCAACTCTGGAAGAGTATTTAAAGAATGCATGGACTTCAGTGGGTGGCCCGGGAGCAATGCTCCATGCTTATTTGTTAACGCAAGGATCCCAACTAACAAAAGCTTCACTTGAGTCCTATAACCATGGCTCTCAGCTGATATATTGGGCATCTCTCACGACTCGACTCAGCGATGATTTGGGCACTTCCAAGGCTGAGGGTGAGAGAGGTGATGTCGCGAAATCCATTCAGTGTTACATGGAAGAAAAAGGCGTATCGGAAGAAGAAGCACAGGATTACATAAAGGGTTTAATCAGCCATTCATGGAAGAAAATGAACCAAGAGAGTGCCAAACCTAGCATACCAAAATCTATTGTAAACATGTGTCTGAATATGGCTCGGACTGCTCACTGCATTTTCGAACACGGGGATGGTATTGGAAACTCAATTGGGGTGACTAAGGATCGTTTGATCTCTTTGATTGCAAATCCTATCCCCCTCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00900 ko00902 ko00909 ko01100 ko01110 ko01130 map00900 map00902 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

602

Amino Acids

67.82

Weight (kDa)

6.05

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 72 - 248 2.3e-45 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 305 - 545 1.8e-82 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 345 - 545 1.5e-51 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 276
AccII CGCG 1 cut(s) 1541
AciI CCGC 1 cut(s) 373
AclWI GGATC 4 cut(s) 400, 1404, 1417, 1773
AcoI YGGCCR 1 cut(s) 1312
AcsI RAATTY 1 cut(s) 676
AcuI CTGAAG 3 cut(s) 371, 743, 1344
AdeI CACNNNGTG 1 cut(s) 276
AfaI GTAC 6 cut(s) 99, 325, 445, 454, 1160, 1279
AfiI CCNNNNNNNGG 1 cut(s) 276
AflII CTTAAG 1 cut(s) 574
AflIII ACRYGT 2 cut(s) 1065, 1683
AgsI TTSAA 8 cut(s) 491, 565, 607, 672, 682, 706, 869, 1124
AhlI ACTAGT 2 cut(s) 62, 440
AjnI CCWGG 3 cut(s) 631, 825, 1086
AloI GAACNNNNNNTCC 4 cut(s) 52, 84, 627, 659
Alw21I GWGCWC 2 cut(s) 759, 900
Alw26I GTCTC 1 cut(s) 139
Alw44I GTGCAC 1 cut(s) 755
AlwI GGATC 4 cut(s) 400, 1404, 1417, 1773
Ama87I CYCGRG 3 cut(s) 1154, 1187, 1372
AoxI GGCC 4 cut(s) 223, 1024, 1312, 1369
ApaLI GTGCAC 1 cut(s) 755
ApeKI GCWGC 4 cut(s) 176, 370, 413, 476
ApoI RAATTY 1 cut(s) 676
ArsI GACNNNNNNTTYG 2 cut(s) 297, 329
Asp700I GAANNNNTTC 1 cut(s) 642
AspA2I CCTAGG 1 cut(s) 770
AspS9I GGNCC 2 cut(s) 218, 1370
AsuC2I CCSGG 3 cut(s) 222, 1373, 1374
AsuHPI GGTGA 5 cut(s) 232, 1021, 1535, 1544, 1768
AsuII TTCGAA 1 cut(s) 1722
AvaI CYCGRG 3 cut(s) 1154, 1187, 1372
AvaII GGWCC 1 cut(s) 218
AvrII CCTAGG 1 cut(s) 770
BaeGI GKGCMC 2 cut(s) 759, 1508
BaeI ACNNNNGTAYC 2 cut(s) 89, 122
BamHI GGATCC 1 cut(s) 1409
BanII GRGCYC 2 cut(s) 900, 1002
BauI CACGAG 1 cut(s) 995
Bbv12I GWGCWC 2 cut(s) 759, 900
BbvCI CCTCAGC 1 cut(s) 1518
BbvI GCAGC 4 cut(s) 188, 382, 425, 488
BccI CCATC 3 cut(s) 210, 955, 1727
BceAI ACGGC 1 cut(s) 1327
BciT130I CCWGG 3 cut(s) 633, 827, 1088
BclI TGATCA 1 cut(s) 163
BcnI CCSGG 3 cut(s) 222, 1373, 1374
BcoDI GTCTC 1 cut(s) 139
BcuI ACTAGT 2 cut(s) 62, 440
BfaI CTAG 9 cut(s) 63, 80, 95, 207, 441, 647, 771, 903, 1659
BfmI CTRYAG 1 cut(s) 174
BfrI CTTAAG 1 cut(s) 574
BisI GCNGC 4 cut(s) 177, 371, 414, 477
BlnI CCTAGG 1 cut(s) 770
BlsI GCNGC 4 cut(s) 178, 372, 415, 478
BmcAI AGTACT 1 cut(s) 325
Bme1390I CCNGG 6 cut(s) 222, 633, 827, 1088, 1373, 1374
Bme18I GGWCC 1 cut(s) 218
BmeT110I CYCGRG 3 cut(s) 1154, 1187, 1372
BmgT120I GGNCC 2 cut(s) 218, 1370
BmiI GGNNCC 3 cut(s) 219, 220, 1411
BmrFI CCNGG 6 cut(s) 222, 633, 827, 1088, 1373, 1374
BmsI GCATC 5 cut(s) 325, 326, 756, 1102, 1481
BplI GAGNNNNNCTC 2 cut(s) 1368, 1400
BpmI CTGGAG 2 cut(s) 781, 857
Bpu10I CCTNAGC 1 cut(s) 1518
Bpu14I TTCGAA 1 cut(s) 1722
BpuEI CTTGAG 3 cut(s) 931, 1118, 1454
BpuMI CCSGG 3 cut(s) 222, 1373, 1374
Bsa29I ATCGAT 2 cut(s) 231, 795
BsaJI CCNNGG 6 cut(s) 363, 622, 770, 1372, 1447, 1512
BsaXI ACNNNNNCTCC 2 cut(s) 627, 657
Bsc4I CCNNNNNNNGG 1 cut(s) 276
Bse1I ACTGG 2 cut(s) 321, 764
Bse3DI GCAATG 2 cut(s) 1204, 1386
BseBI CCWGG 3 cut(s) 633, 827, 1088
BseCI ATCGAT 2 cut(s) 231, 795
BseDI CCNNGG 6 cut(s) 363, 622, 770, 1372, 1447, 1512
BseGI GGATG 7 cut(s) 67, 166, 202, 317, 771, 1096, 1738
BseLI CCNNNNNNNGG 1 cut(s) 276
BseMI GCAATG 2 cut(s) 1204, 1386
BseMII CTCAG 6 cut(s) 48, 537, 1023, 1469, 1504, 1509
BseNI ACTGG 2 cut(s) 321, 764
BseRI GAGGAG 4 cut(s) 7, 10, 13, 16
BseSI GKGCMC 2 cut(s) 759, 1508
BseX3I CGGCCG 1 cut(s) 1312
BseXI GCAGC 4 cut(s) 188, 382, 425, 488
BsgI GTGCAG 1 cut(s) 389
Bsh1236I CGCG 1 cut(s) 1541
Bsh1285I CGRYCG 1 cut(s) 1315
BshFI GGCC 4 cut(s) 225, 1026, 1314, 1371
BshVI ATCGAT 2 cut(s) 231, 795
BsiEI CGRYCG 1 cut(s) 1315
BsiHKAI GWGCWC 2 cut(s) 759, 900
BsiHKCI CYCGRG 3 cut(s) 1154, 1187, 1372
BsiSI CCGG 2 cut(s) 222, 1373
BslFI GGGAC 3 cut(s) 204, 606, 965
BslI CCNNNNNNNGG 1 cut(s) 276
BsmAI GTCTC 1 cut(s) 139
BsmFI GGGAC 3 cut(s) 204, 606, 965
BsmI GAATGC 4 cut(s) 81, 633, 1019, 1354
BsnI GGCC 4 cut(s) 225, 1026, 1314, 1371
BsoBI CYCGRG 3 cut(s) 1154, 1187, 1372
Bsp119I TTCGAA 1 cut(s) 1722
Bsp1286I GDGCHC 4 cut(s) 759, 900, 1002, 1508
Bsp1407I TGTACA 1 cut(s) 1277
Bsp143I GATC 6 cut(s) 163, 405, 463, 1409, 1765, 1773
Bsp19I CCATGG 1 cut(s) 1447
Bsp68I TCGCGA 1 cut(s) 1541
BspACI CCGC 1 cut(s) 373
BspANI GGCC 4 cut(s) 225, 1026, 1314, 1371
BspCNI CTCAG 6 cut(s) 47, 536, 1022, 1468, 1503, 1510
BspDI ATCGAT 2 cut(s) 231, 795
BspFNI CGCG 1 cut(s) 1541
BspLI GGNNCC 3 cut(s) 219, 220, 1411
BspMAI CTGCAG 1 cut(s) 178
BspPI GGATC 4 cut(s) 400, 1404, 1417, 1773
BspT104I TTCGAA 1 cut(s) 1722
BspTI CTTAAG 1 cut(s) 574
BsrDI GCAATG 2 cut(s) 1204, 1386
BsrGI TGTACA 1 cut(s) 1277
BsrI ACTGG 2 cut(s) 321, 764
BssECI CCNNGG 6 cut(s) 363, 622, 770, 1372, 1447, 1512
BssMI GATC 6 cut(s) 163, 405, 463, 1409, 1765, 1773
BssSI CACGAG 1 cut(s) 995
BssT1I CCWWGG 4 cut(s) 622, 770, 1447, 1512
Bst2BI CACGAG 1 cut(s) 995
Bst2UI CCWGG 3 cut(s) 633, 827, 1088
Bst4CI ACNGT 4 cut(s) 276, 584, 1054, 1302
Bst6I CTCTTC 2 cut(s) 112, 1329
BstAFI CTTAAG 1 cut(s) 574
BstAPI GCANNNNNTGC 1 cut(s) 742
BstAUI TGTACA 1 cut(s) 1277
BstBI TTCGAA 1 cut(s) 1722
BstC8I GCNNGC 1 cut(s) 340
BstDSI CCRYGG 1 cut(s) 1447
BstF5I GGATG 7 cut(s) 67, 166, 202, 317, 771, 1096, 1738
BstFNI CGCG 1 cut(s) 1541
BstKTI GATC 6 cut(s) 166, 408, 466, 1412, 1768, 1776
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 6 cut(s) 163, 405, 463, 1409, 1765, 1773
BstMCI CGRYCG 1 cut(s) 1315
BstMWI GCNNNNNNNGC 6 cut(s) 49, 314, 733, 742, 1023, 1706
BstNI CCWGG 3 cut(s) 633, 827, 1088
BstNSI RCATGY 3 cut(s) 269, 1069, 1687
BstSCI CCNGG 6 cut(s) 220, 631, 825, 1086, 1371, 1372
BstSFI CTRYAG 1 cut(s) 174
BstSLI GKGCMC 2 cut(s) 759, 1508
BstUI CGCG 1 cut(s) 1541
BstV1I GCAGC 4 cut(s) 188, 382, 425, 488
BstX2I RGATCY 2 cut(s) 405, 1409
BstYI RGATCY 2 cut(s) 405, 1409
BstZI CGGCCG 1 cut(s) 1312
Bsu15I ATCGAT 2 cut(s) 231, 795
BsuRI GGCC 4 cut(s) 225, 1026, 1314, 1371
BsuTUI ATCGAT 2 cut(s) 231, 795
BtgI CCRYGG 1 cut(s) 1447
BtgZI GCGATG 1 cut(s) 1509
BtsCI GGATG 7 cut(s) 67, 166, 202, 317, 771, 1096, 1738
BtsI GCAGTG 4 cut(s) 89, 740, 830, 1711
BtsIMutI CAGTG 7 cut(s) 89, 740, 757, 830, 1368, 1559, 1711
BtuMI TCGCGA 1 cut(s) 1541
Cac8I GCNNGC 1 cut(s) 340
Cfr13I GGNCC 2 cut(s) 218, 1370
Cfr9I CCCGGG 1 cut(s) 1372
ClaI ATCGAT 2 cut(s) 231, 795
Csp6I GTAC 6 cut(s) 98, 324, 444, 453, 1159, 1278
CviQI GTAC 6 cut(s) 98, 324, 444, 453, 1159, 1278
DpnI GATC 6 cut(s) 165, 407, 465, 1411, 1767, 1775
DpnII GATC 6 cut(s) 163, 405, 463, 1409, 1765, 1773
DraI TTTAAA 3 cut(s) 928, 1236, 1344
DraIII CACNNNGTG 1 cut(s) 276
EaeI YGGCCR 1 cut(s) 1312
EagI CGGCCG 1 cut(s) 1312
Eam1104I CTCTTC 2 cut(s) 112, 1329
EarI CTCTTC 2 cut(s) 112, 1329
Ecl136II GAGCTC 1 cut(s) 898
EclXI CGGCCG 1 cut(s) 1312
Eco130I CCWWGG 4 cut(s) 622, 770, 1447, 1512
Eco147I AGGCCT 1 cut(s) 1026
Eco24I GRGCYC 2 cut(s) 900, 1002
Eco32I GATATC 1 cut(s) 799
Eco47I GGWCC 1 cut(s) 218
Eco52I CGGCCG 1 cut(s) 1312
Eco53kI GAGCTC 1 cut(s) 898
Eco57I CTGAAG 3 cut(s) 371, 743, 1344
Eco88I CYCGRG 3 cut(s) 1154, 1187, 1372
EcoICRI GAGCTC 1 cut(s) 898
EcoO109I RGGNCCY 1 cut(s) 218
EcoRII CCWGG 3 cut(s) 631, 825, 1086
EcoRV GATATC 1 cut(s) 799
EcoT14I CCWWGG 4 cut(s) 622, 770, 1447, 1512
EcoT22I ATGCAT 1 cut(s) 1354
EcoT38I GRGCYC 2 cut(s) 900, 1002
ErhI CCWWGG 4 cut(s) 622, 770, 1447, 1512
FalI AAGNNNNNCTT 2 cut(s) 1416, 1448
FaqI GGGAC 3 cut(s) 204, 606, 965
FbaI TGATCA 1 cut(s) 163
Fnu4HI GCNGC 4 cut(s) 177, 371, 414, 477
FokI GGATG 7 cut(s) 54, 153, 189, 304, 778, 1103, 1745
FriOI GRGCYC 2 cut(s) 900, 1002
Fsp4HI GCNGC 4 cut(s) 177, 371, 414, 477
FspBI CTAG 9 cut(s) 63, 80, 95, 207, 441, 647, 771, 903, 1659
GluI GCNGC 4 cut(s) 177, 371, 414, 477
GsuI CTGGAG 2 cut(s) 781, 857
HaeIII GGCC 4 cut(s) 225, 1026, 1314, 1371
HapII CCGG 2 cut(s) 222, 1373
HincII GTYRAC 2 cut(s) 696, 1401
HindII GTYRAC 2 cut(s) 696, 1401
HindIII AAGCTT 3 cut(s) 353, 559, 1425
HinfI GANTC 9 cut(s) 4, 233, 260, 815, 1083, 1317, 1436, 1483, 1488
HpaI GTTAAC 1 cut(s) 1401
HpaII CCGG 2 cut(s) 222, 1373
HphI GGTGA 5 cut(s) 232, 1021, 1535, 1544, 1768
Hpy166II GTNNAC 4 cut(s) 696, 757, 1401, 1681
Hpy188III TCNNGA 6 cut(s) 647, 995, 1154, 1331, 1480, 1540
Hpy8I GTNNAC 4 cut(s) 696, 757, 1401, 1681
Hpy99I CGWCG 1 cut(s) 713
HpyAV CCTTC 3 cut(s) 247, 548, 928
HpyCH4III ACNGT 4 cut(s) 276, 584, 1054, 1302
HpyCH4IV ACGT 1 cut(s) 297
HpyF10VI GCNNNNNNNGC 6 cut(s) 49, 314, 733, 742, 1023, 1706
HpySE526I ACGT 1 cut(s) 297
KflI GGGWCCC 1 cut(s) 218
Ksp22I TGATCA 1 cut(s) 163
KspAI GTTAAC 1 cut(s) 1401
Kzo9I GATC 6 cut(s) 163, 405, 463, 1409, 1765, 1773
LmnI GCTCC 3 cut(s) 347, 1376, 1389
Lsp1109I GCAGC 4 cut(s) 188, 382, 425, 488
LweI GCATC 5 cut(s) 325, 326, 756, 1102, 1481
MaeI CTAG 9 cut(s) 63, 80, 95, 207, 441, 647, 771, 903, 1659
MaeII ACGT 1 cut(s) 297
MaeIII GTNAC 4 cut(s) 421, 992, 1556, 1756
MalI GATC 6 cut(s) 165, 407, 465, 1411, 1767, 1775
MboI GATC 6 cut(s) 163, 405, 463, 1409, 1765, 1773
MboII GAAGA 9 cut(s) 129, 426, 458, 650, 1346, 1577, 1595, 1598, 1642
MfeI CAATTG 1 cut(s) 1749
MflI RGATCY 2 cut(s) 405, 1409
MhlI GDGCHC 4 cut(s) 759, 900, 1002, 1508
MluCI AATT 8 cut(s) 485, 676, 848, 882, 1004, 1119, 1202, 1749
MlyI GAGTC 4 cut(s) 809, 1445, 1477, 1482
MmeI TCCRAC 1 cut(s) 900
Mph1103I ATGCAT 1 cut(s) 1354
MroXI GAANNNNTTC 1 cut(s) 642
MslI CAYNNNNRTG 1 cut(s) 1731
MspA1I CMGCKG 2 cut(s) 373, 1459
MspCI CTTAAG 1 cut(s) 574
MspI CCGG 2 cut(s) 222, 1373
MspR9I CCNGG 6 cut(s) 222, 633, 827, 1088, 1373, 1374
MssI GTTTAAAC 1 cut(s) 1236
MunI CAATTG 1 cut(s) 1749
Mva1269I GAATGC 4 cut(s) 81, 633, 1019, 1354
MvaI CCWGG 3 cut(s) 633, 827, 1088
MvnI CGCG 1 cut(s) 1541
MwoI GCNNNNNNNGC 6 cut(s) 49, 314, 733, 742, 1023, 1706
NciI CCSGG 3 cut(s) 222, 1373, 1374
NcoI CCATGG 1 cut(s) 1447
NdeII GATC 6 cut(s) 163, 405, 463, 1409, 1765, 1773
NlaIV GGNNCC 3 cut(s) 219, 220, 1411
NmuCI GTSAC 2 cut(s) 992, 1756
NruI TCGCGA 1 cut(s) 1541
NsiI ATGCAT 1 cut(s) 1354
NspI RCATGY 3 cut(s) 269, 1069, 1687
NspV TTCGAA 1 cut(s) 1722
PaeR7I CTCGAG 1 cut(s) 1187
PceI AGGCCT 1 cut(s) 1026
PciI ACATGT 2 cut(s) 1065, 1683
PctI GAATGC 4 cut(s) 81, 633, 1019, 1354
PdmI GAANNNNTTC 1 cut(s) 642
PfeI GAWTC 5 cut(s) 4, 233, 260, 1083, 1317
PflMI CCANNNNNTGG 1 cut(s) 276
PfoI TCCNGGA 1 cut(s) 1086
PkrI GCNGC 4 cut(s) 178, 372, 415, 478
PleI GAGTC 4 cut(s) 809, 1444, 1477, 1482
PmeI GTTTAAAC 1 cut(s) 1236
PpsI GAGTC 4 cut(s) 809, 1444, 1477, 1482
PpuMI RGGWCCY 1 cut(s) 218
PscI ACATGT 2 cut(s) 1065, 1683
Psp124BI GAGCTC 1 cut(s) 900
Psp5II RGGWCCY 1 cut(s) 218
Psp6I CCWGG 3 cut(s) 631, 825, 1086
PspGI CCWGG 3 cut(s) 631, 825, 1086
PspN4I GGNNCC 3 cut(s) 219, 220, 1411
PspPI GGNCC 2 cut(s) 218, 1370
PspPPI RGGWCCY 1 cut(s) 218
PspXI VCTCGAGB 1 cut(s) 1187
PstI CTGCAG 1 cut(s) 178
PsuI RGATCY 2 cut(s) 405, 1409
PvuII CAGCTG 1 cut(s) 1459
RruI TCGCGA 1 cut(s) 1541
RsaI GTAC 6 cut(s) 99, 325, 445, 454, 1160, 1279
RsaNI GTAC 6 cut(s) 98, 324, 444, 453, 1159, 1278
RseI CAYNNNNRTG 1 cut(s) 1731
SacI GAGCTC 1 cut(s) 900
SatI GCNGC 4 cut(s) 177, 371, 414, 477
Sau3AI GATC 6 cut(s) 163, 405, 463, 1409, 1765, 1773
Sau96I GGNCC 2 cut(s) 218, 1370
ScaI AGTACT 1 cut(s) 325
SchI GAGTC 4 cut(s) 809, 1445, 1477, 1482
ScrFI CCNGG 6 cut(s) 222, 633, 827, 1088, 1373, 1374
SduI GDGCHC 4 cut(s) 759, 900, 1002, 1508
SfaNI GCATC 5 cut(s) 325, 326, 756, 1102, 1481
SfcI CTRYAG 1 cut(s) 174
Sfr274I CTCGAG 1 cut(s) 1187
SfuI TTCGAA 1 cut(s) 1722
SinI GGWCC 1 cut(s) 218
SlaI CTCGAG 1 cut(s) 1187
SmaI CCCGGG 1 cut(s) 1374
SmiMI CAYNNNNRTG 1 cut(s) 1731
SmlI CTYRAG 5 cut(s) 574, 946, 1097, 1187, 1433
SmoI CTYRAG 5 cut(s) 574, 946, 1097, 1187, 1433
SpeI ACTAGT 2 cut(s) 62, 440
Sse9I AATT 8 cut(s) 485, 676, 848, 882, 1004, 1119, 1202, 1749
SseBI AGGCCT 1 cut(s) 1026
SsiI CCGC 1 cut(s) 373
SspI AATATT 1 cut(s) 702
SspMI CTAG 9 cut(s) 63, 80, 95, 207, 441, 647, 771, 903, 1659
SstI GAGCTC 1 cut(s) 900
StuI AGGCCT 1 cut(s) 1026
StyD4I CCNGG 6 cut(s) 220, 631, 825, 1086, 1371, 1372
StyI CCWWGG 4 cut(s) 622, 770, 1447, 1512
TaaI ACNGT 4 cut(s) 276, 584, 1054, 1302
TaiI ACGT 1 cut(s) 300
TaqI TCGA 6 cut(s) 231, 795, 932, 1188, 1486, 1722
TasI AATT 8 cut(s) 485, 676, 848, 882, 1004, 1119, 1202, 1749
TatI WGTACW 4 cut(s) 97, 323, 1158, 1277
TfiI GAWTC 5 cut(s) 4, 233, 260, 1083, 1317
TscAI CASTG 7 cut(s) 96, 747, 764, 837, 1368, 1559, 1718
TseFI GTSAC 2 cut(s) 992, 1756
TseI GCWGC 4 cut(s) 176, 370, 413, 476
Tsp45I GTSAC 2 cut(s) 992, 1756
TspDTI ATGAA 9 cut(s) 17, 273, 458, 624, 781, 1178, 1215, 1614, 1652
TspMI CCCGGG 1 cut(s) 1372
TspRI CASTG 7 cut(s) 96, 747, 764, 837, 1368, 1559, 1718
Van91I CCANNNNNTGG 1 cut(s) 276
Vha464I CTTAAG 1 cut(s) 574
VneI GTGCAC 1 cut(s) 755
VpaK11BI GGWCC 1 cut(s) 218
XapI RAATTY 1 cut(s) 676
XbaI TCTAGA 1 cut(s) 646
XceI RCATGY 3 cut(s) 269, 1069, 1687
XhoI CTCGAG 1 cut(s) 1187
XmaI CCCGGG 1 cut(s) 1372
XmaJI CCTAGG 1 cut(s) 770
XmnI GAANNNNTTC 1 cut(s) 642
XspI CTAG 9 cut(s) 63, 80, 95, 207, 441, 647, 771, 903, 1659
ZrmI AGTACT 1 cut(s) 325
Zsp2I ATGCAT 1 cut(s) 1354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.