FvH4_6g51330

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001894
Physical Location & Seq
Reverse (-)
38054867 .. 38067504
12638 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g51330.t8

Sequence Viewer

Length: 5559 bp
ATGGACTCTTACACTCCCTTACTGGAGAAAACCCGGGTCCCCCAACCCTCCCTCCAAAAGTATGCCGTGATTTCAATCTTCTCGAAGCTCCGATCCGCCCCGAAGCACCTAGACTCCGACTCCGAACCCGGACGTGACGCCATTTCCCAGTGCCTCCATTCCACTTCCCCCGCCGTCGTCGACCAATCGGTTCGCGAGCTCTGCGGCCTCGTCGCCGACGGAAAATCCCGATTCGATGTCCGCCGCGGCTTGCTGGAGCTCCAGTCCGCGCTCGAAGGATCCGATTCCAAGTTCGTCAGCCTCTTCGTGAAAGGTTTGGGGTTTTTGGTCCGGTTAGGGTTTCAGAAGAGCAATGGCGACTGGTGTTTCAGCAACACAGAGTCTCATCCCTTCGTTAAGGTTCTTTCGTGCCGAAGCGATGTGGAGGATGAACTTGTGCAGCAAGTGTTACTGTTTACGGCACAGAACAGGCAGTTGGGAATGGTGGAAGTATGCAAGTTCTTGAGACCGTTTCTGAACTATACGGTTTTGAGGATACCGTTTACGGAGAATTCCTCTATGTTTGCTAGGCGTTTGGTATCGTCAATGGCGTCTCTTTGTTGCTCGATTCCTGTTGATGCAATGCCTGTGCTTAGATTGTTGACAGAATGTCTTCAATACGTTCCGCACAAGAGTTCAGAAGATTTCAGGAATTTCACATATTTAGCAGAGTGTATGGTGGATGCATATATAGTGGTTTTGAGGCATTTAGCCGGAACTAGATCGGTGATGGCTGAGGTTCAACTATGTGGTTTGGAACTGTTTGAGAACCTTATATCGATTTGCACAACTGGTCATAGACACTCCAGTTCAATTGAGCCTCTAGTTGAACTGTCCAAGAACTTATTGTTGTCCCAGAAGGATAATGGGTTCCCTTATGCACGTAAGTTATCATCAGCCATGCTTTCATTATTTATCATCCTCGTCCAATCAGAGCTCGAGCACGAACAACTTTCAACATTGAAACTTCTGCACTTTCTCTTGAAGTGGAAGTATGGAAACGTAAATGGTGTAGACAAAAGTGGAGGTGCTATGAGCGAGGAGCTTTTGTTCATATTTCCCGTTGTTGGTCTACTGTCATCTCCCTCTAAACATGTCAAAGTAGCGGCAACAGATCTGCTTGTCATGCTAGAAAGACTTTTAGTAAGAGTTTTGGTAGCACCAAAGGATAAACCAGCAAAAGTAGGGTATCCCTCTTTAAGCACACCTGGATCTATTGTGTTTAGAATCTTACAGCATCTCTGGTTTCAGAATTCCTATTCATTGTCCAGTTTGTTTTTAAGTTTTTCTTGTATTGGTAATGCTATTGGCAAGGAGATTCATGATGTACCAAGATCCTGGGCTTCTCATTTACAAGAGTATACTCTATCGATTGTTGACAAACGAAAATCCTCCCTACCTCTCCCGCAGACTCAAGAAATATACATAACTGAGATGCCCTCGTTACTTTCTGCCATTGCTGGTCTTTTAGTGATGCATGAGGTAGAGGGAGGCACTGCTGTAGATTCTTTGGCTGCCATTTCCACAATGGATCCTAAATTGGGAGCTCAAATGTTGCTGGCTATCCTATTTTACAATAACATTTTCACTAGAAAAGATATAAGTCGCTTTACTATGTTGCCAAAACTTTTGACAATGCTTCCAGCACTTGCCTCACAGTCTGTGATGATTCCTCTTGTAGTTCAGACTATCTTGCCAATGCTTCAGAAGGATGCGAAACCAACTTTACATGCGACAGCCATTCGCTTGCTTTGTCAGACCTGGGAGACCAATGGCCGTGCCTTTGGGAGTTTGCAGGGAGTGCTGGTTCCAAAAGGATTCACTGACTTCAAGTCTGAGAGAAATATATGCATCAGCATGGCTGCATCAGTTCGCGATGTTTGTAGAAAAAATCCTGATAGGGGTGTGGACCTCATCTTATCTGTCTCGGCTTGCATTGAGAACAATGATCCGATTATTCAAGCTATTGGGTTTCAAAGTCTTGCCCACCTTTGTGAAGCTGATGTAATTGATTTTTACACAGCATGGGATGTCATCAGGAAGCATGTTCTGGATTACACTGTAGACCCAAATCTTGCTCAGAGCCTTTGCCTTCTTCTGAGGTGGGGTGCAATGGACGCTGAAGCACATCCTGAAGCCTCAAAAGACATCTTACAGATCATATGGAGTGTTAGCATCTCTACTCATCCTGGTCTGGAGACACAGTGGGCAAAGGCAAGGGCTTCTTCCTTTAAGGCCATGGCCCAATTTGAGCTATCACATATTGAACAAGCAATTCAAGACTTCAAGAAGAGGAACCTGGAGTTGCTTTCTTCTGAACCAAGCATCACTGTACTCAATGCCATGGAAGAACTTCTGGTCAAGATAATAACATATGAGCACCTAACAAGGCGAAGATTAGTTAAGGAGAAAAGAGTTGCAGGAAGTAAGATCGAGAAACTGCTGGATGTGTTTCCTCAGGTGATCTTTTCTTCAGGGAAAAAATGTGACGCGAGGGACTTACCTGGTGCAGCTTTGCTATGTCTTTCCTTTACTCCTAAAGATGTGAATACCCAAGGGCTGTCTAGGGGCCTACGAGATATACATGGTGGATTTGAGAATGCTCTGGTGGAACTAACTTCATCCCTTCAGCTCTCTAGAAATCTTTTTGTTGCCCTTATTTCACTTGAATCATGGAAATCTTTCATGCGCCGTTGGCTGAGGGCAGACATCTTGTTCTTTGATGCTAAAGTGTCATCTGTCATCTTGGATAAAACTACTAAAGCTGCTAGTGACATTTTAAAGAGTCTGATAAAAATAGCAGAAGAGGCTCTTCCTAGATCTGCTGAAAACATTGCGCTAGCTGTTGGTGCCCTTTGTGCGGTCTTGCCTCCGTCTGCCCATACTGTTAAAGCAGCTGCCTCAAAATTTCTATTGAATTGGTTGGTCCAGCCTGAGCATGAACACCGCAAATGGTCAGCTGCAATTTCTCTTGGATTGATCTCAAGCTGCCTACACATTACTGATCATAAGCAAAAGTTTGAGAATGTCTCGAGACTTGTTGAGGTGATGTATAGTAGCAAAAGCACCCTTGTCAAAGGAGCCTGTGGGGTTGGCTTGGGGTTTTCCTGCCAAGATCTTCTGACCAGGGCTGATTCTGCTGATAATTCTAGTACGGAGAAAGACTCAGAAAAGATGTCAGAAAGGGAATTGCTTGGTGACATAGTCAAGGCCTTATTACGTATGATAAGTGAGATCACTCAGGTGGCTCCTGATATTTTTGAAGTTCTCTCTGCTTATTTCCCACCTAGCAGATATGATGTAGATACAAGTACATCTGCTCAGTGGTCAAATGAGAATTGCGATAACTCTCTTGAAGATATATGGGGTATTGCTGGACTCGTTCTTGGTTTGGCGAGTTCTATTTGTGCAATGTACAGAGCTGGGGCGCATGATGCCATTGTCAAGATAAAAGAGGTGATCGTATCATGGGTTCCACATATAAATAGACTTGTTCAAGGCTCTGATTCTTACTCTGGGGGTTCTGAGATAGTTTTGTCTGTAGGAGCTTGTCTTGCGATTCCCATTGTAGTGGCATTCTGCCAAAGAGTAGAATTGATGGATGAGATTGAAGTAAACCATTTAATAAATGGATACAGGGAGCTCATTTCTGAATTATTGTCTATCAAAAAATCTGGCACTTTCTATCACAGCTTATTGATGGCATCATGCATAGGGGCAGGCAGTCTTCTAGCCTGTATAATGAATGAAGGTGTGCATGCTATTGAAGTTGAGCGTGTAAATCGTATACTAGAATTATTAAAAAGATGCTATTCCAGTCCTTTCCCTCCTTTAGTGCATTTTGGAGGAATGCTTGGAGTTGTTAACGCTATGGGAGCAGGTGCTGGGATCCTGTCTGATAGGCTTCCGCTAACCTCCTTACAGGCAGCTTTTGAACCGAAGGAATCTGGCTATGTCATGGGTCCTCTACTCTCAAATCCTGCTTGTGAGCAGCATTTGACATCATTGATGCAGGATATATTTCTTGTGGCACAGAAATCTGATGATCATCAATTGCAACAATATGCTGCATGGGCAGCTTCGTTTCTTCGAAATCATTTGTTGTCCAAGGATGTTGACAATAGTATAAATGCAGATTCAGGTGCTTCAAAGTCTGTTTCACAAAGTTTTCCAGATGACAGTTTAGTTATGATGCTTTCTTCATGGCTAATGTATCTCAACTTTACCAGGACAGGTAGTGTTGCTCATGTTGGCACAGTAATAACTGCGGTAAGGTGTCTTTCACAAGCTCCTAGGTTGCCAACTTTGGACTGGGGAGCAATCATCAGACGTGGCATGAGATATGAGGCACAAGTCACGGAGATGTTACCAACAGAATCATCATTCCGTAAAGGAATTCTTAGGGAGGAGTGTCTGAAGTTCTCATTAGCTCATGCAAATAAATTTGATCAACTCCTAAGTTTCCTTGATGAGCTATCTGACTTGTCCAGATTCAGTACACTAGAGTTGAATCTGCAATCTTGTGTTCTCAACCATCTTGCTGACCTCATAAAAGTATTCTCCAGTTCCAGGCTTGAGAAACTATTCGATGATCTCTGCAGCTACTTCTCTTCAGCTACTTCATGTCAATCATATGACACTGATGAGACAAAATTGTTACGGATTTCATGCTGGAAGGGTCTCTACAAATGTTTAGACGAGGCTTCTCTTGATTCTCTCGAGTACATATCCCACATAGAAAAATGCATGGAGGTGTTGTTTTCCTTGTTACCTGCTAGGCAGTTGGCTACCATGGTAGATCAGTTAAATTATTTGAAGGAGTGGTCTGAGGCAGTTACATGCTTAGGAAAGGCTCGTAAACATTGGTTGGTGAATTTCCTGCAGGACGGCCTACGACCAAGAGATGACCGGCTTGTAGAAGGTCTGAAAAAAATTCAAGCCAAAGCTAAGCTAGTCAGATTTGGTTTTATCCCTTTGACTGAGCTGGGAAGACTAAAAGCTCTAATATTGAATACTGAATCAGATGGCATATGGGACGTGCTTGTGGAAGTTGTAGCAGCTTTGCAAGATGCAGACGGAAGTATCAAACGACAGTGGCTCATTGATGCAGCTGAAATTAGCTGTGTATCAAGTTATCCCTCCACGGCGTTGAAGTTTCTTGGGTTACTCTCCGGTAGCTGGAGCAAATATATGCCACTTCTGATTTTGGACCAACAAAGTGTGCTGAGTGATTTACCAGTCACTCTTTCTTCTCTTCTCTCACACTCTAGTTGGGGAGCTGTTGTGGAATCTGTCATGTCTTCTCTTTTTGCATCAACTGAGCGAATTTACAATTGGACCACACATGTGGCACCGGGTAAGGATATGCCGCCTGACATGCAACCTATTGATGAAAGCGAGAATCCCATGGCTGGTTTCCTTTTGTGTGTGATGCATTCTACCTGTGTAACCTTGAAGGACTACTTGTCTCTTGAGAAGCAGCTTAAGCTTTCCAATATGGATATAACATTGGTCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3730

Protein Analysis

1853

Amino Acids

205.24

Weight (kDa)

6.23

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3730 PF12530 68 - 274 1.3e-63 Focadhesin/RST1, DUF3730
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3908
Acc36I ACCTGC 2 cut(s) 3908, 4819
AccB1I GGYRCC 2 cut(s) 2888, 5389
AccI GTMKAC 6 cut(s) 180, 1053, 1111, 1400, 2103, 3826
AccII CGCG 5 cut(s) 195, 246, 269, 1914, 2531
AcoI YGGCCR 1 cut(s) 1813
AcsI RAATTY 9 cut(s) 550, 691, 1291, 2945, 4434, 4481, 4912, 4971, 5364
AcuI CTGAAG 7 cut(s) 1727, 2181, 2193, 2496, 2651, 4475, 4635
AcyI GRCGYC 2 cut(s) 138, 590
AfaI GTAC 7 cut(s) 1368, 2373, 3193, 3352, 3455, 4537, 4763
AfiI CCNNNNNNNGG 7 cut(s) 1106, 1580, 1940, 2899, 4608, 5217, 5450
AflII CTTAAG 1 cut(s) 5522
AflIII ACRYGT 2 cut(s) 1132, 5383
AhdI GACNNNNNGTC 3 cut(s) 648, 1870, 5503
AjiI CACGTC 3 cut(s) 134, 4370, 5077
AjnI CCWGG 9 cut(s) 1246, 1376, 1799, 2227, 2337, 2542, 3164, 4265, 4607
AjuI GAANNNNNNNTTGG 4 cut(s) 458, 490, 5356, 5388
AleI CACNNNNGTG 3 cut(s) 2031, 3281, 5384
AloI GAACNNNNNNTCC 6 cut(s) 893, 925, 1073, 1105, 1830, 1862
Alw21I GWGCWC 7 cut(s) 201, 261, 978, 984, 1588, 2421, 3684
AlwNI CAGNNNCTG 1 cut(s) 3923
Ama87I CYCGRG 4 cut(s) 33, 977, 3070, 4757
AoxI GGCC 7 cut(s) 205, 1813, 2274, 2280, 2608, 3249, 4927
ApoI RAATTY 9 cut(s) 550, 691, 1291, 2945, 4434, 4481, 4912, 4971, 5364
Asp700I GAANNNNTTC 4 cut(s) 651, 2391, 2720, 4622
AspA2I CCTAGG 1 cut(s) 4331
AspLEI GCGC 4 cut(s) 271, 2730, 2878, 3469
AspS9I GGNCC 9 cut(s) 37, 328, 1948, 2281, 2608, 2965, 4001, 5248, 5376
AsuC2I CCSGG 4 cut(s) 34, 35, 129, 5394
AsuHPI GGTGA 6 cut(s) 778, 2512, 3097, 3248, 3508, 4921
AsuII TTCGAA 1 cut(s) 4129
AsuNHI GCTAGC 1 cut(s) 2878
AvaI CYCGRG 4 cut(s) 33, 977, 3070, 4757
AvaII GGWCC 7 cut(s) 37, 328, 1948, 2965, 4001, 5248, 5376
AvrII CCTAGG 1 cut(s) 4331
AxyI CCTNAGG 1 cut(s) 2496
BaeGI GKGCMC 1 cut(s) 2893
BaeI ACNNNNGTAYC 2 cut(s) 5104, 5137
BamHI GGATCC 3 cut(s) 278, 1570, 3927
BanI GGYRCC 2 cut(s) 2888, 5389
BanII GRGCYC 5 cut(s) 201, 261, 978, 1588, 3684
BarI GAAGNNNNNNTAC 2 cut(s) 4706, 4738
BbsI GAAGAC 4 cut(s) 644, 3758, 5035, 5331
Bbv12I GWGCWC 7 cut(s) 201, 261, 978, 984, 1588, 2421, 3684
BbvCI CCTCAGC 2 cut(s) 774, 2738
BccI CCATC 5 cut(s) 763, 3631, 3733, 4581, 5057
BceAI ACGGC 7 cut(s) 50, 158, 474, 1800, 2715, 4942, 5199
BciT130I CCWGG 9 cut(s) 1248, 1378, 1801, 2229, 2339, 2544, 3166, 4267, 4609
BciVI GTATCC 3 cut(s) 528, 1239, 3665
BclI TGATCA 3 cut(s) 3043, 4084, 4486
BcnI CCSGG 4 cut(s) 34, 35, 129, 5394
BfmI CTRYAG 5 cut(s) 1539, 2100, 3579, 4636, 4919
BfrI CTTAAG 1 cut(s) 5522
BfuAI ACCTGC 2 cut(s) 3908, 4819
BfuI GTATCC 3 cut(s) 528, 1239, 3665
BglII AGATCT 3 cut(s) 1153, 2858, 3154
BlnI CCTAGG 1 cut(s) 4331
BlpI GCTNAGC 1 cut(s) 4986
Bme18I GGWCC 7 cut(s) 37, 328, 1948, 2965, 4001, 5248, 5376
BmeRI GACNNNNNGTC 3 cut(s) 648, 1870, 5503
BmeT110I CYCGRG 4 cut(s) 33, 977, 3070, 4757
BmgBI CACGTC 3 cut(s) 134, 4370, 5077
BmgT120I GGNCC 9 cut(s) 37, 328, 1948, 2281, 2608, 2965, 4001, 5248, 5376
BmrI ACTGGG 2 cut(s) 142, 4360
BmtI GCTAGC 1 cut(s) 2882
BmuI ACTGGG 2 cut(s) 142, 4360
BpiI GAAGAC 4 cut(s) 644, 3758, 5035, 5331
BplI GAGNNNNNCTC 8 cut(s) 539, 571, 1463, 1495, 3053, 3085, 3188, 3220
BpmI CTGGAG 8 cut(s) 44, 245, 275, 829, 2255, 2360, 4585, 5239
Bpu10I CCTNAGC 4 cut(s) 774, 2738, 2973, 4882
Bpu1102I GCTNAGC 1 cut(s) 4986
Bpu14I TTCGAA 1 cut(s) 4129
BpuEI CTTGAG 5 cut(s) 523, 1437, 3007, 4634, 5531
BpuMI CCSGG 4 cut(s) 34, 35, 129, 5394
Bsa29I ATCGAT 2 cut(s) 818, 1409
BsaAI YACGTR 2 cut(s) 923, 3260
BsaBI GATNNNNATC 2 cut(s) 74, 4086
BsaHI GRCGYC 2 cut(s) 138, 590
BsaI GGTCTC 3 cut(s) 499, 1799, 4724
BsaWI WCCGGW 2 cut(s) 330, 5210
Bsc4I CCNNNNNNNGG 7 cut(s) 1106, 1580, 1940, 2899, 4608, 5217, 5450
Bse118I RCCGGY 1 cut(s) 4947
Bse21I CCTNAGG 1 cut(s) 2496
Bse3DI GCAATG 6 cut(s) 358, 627, 1494, 2157, 2871, 3456
Bse8I GATNNNNATC 2 cut(s) 74, 4086
BseBI CCWGG 9 cut(s) 1248, 1378, 1801, 2229, 2339, 2544, 3166, 4267, 4609
BseCI ATCGAT 2 cut(s) 818, 1409
BseJI GATNNNNATC 2 cut(s) 74, 4086
BseLI CCNNNNNNNGG 7 cut(s) 1106, 1580, 1940, 2899, 4608, 5217, 5450
BseMI GCAATG 6 cut(s) 358, 627, 1494, 2157, 2871, 3456
BseRI GAGGAG 2 cut(s) 1094, 4460
BseSI GKGCMC 1 cut(s) 2893
BseYI CCCAGC 3 cut(s) 3461, 3923, 5023
BsgI GTGCAG 3 cut(s) 458, 995, 2568
Bsh1236I CGCG 5 cut(s) 195, 246, 269, 1914, 2531
BshFI GGCC 7 cut(s) 207, 1815, 2276, 2282, 2610, 3251, 4929
BshNI GGYRCC 2 cut(s) 2888, 5389
BshVI ATCGAT 2 cut(s) 818, 1409
BsiHKAI GWGCWC 7 cut(s) 201, 261, 978, 984, 1588, 2421, 3684
BsiHKCI CYCGRG 4 cut(s) 33, 977, 3070, 4757
BsiSI CCGG 7 cut(s) 34, 129, 331, 753, 4948, 5211, 5393
BslFI GGGAC 4 cut(s) 23, 877, 2549, 5087
BslI CCNNNNNNNGG 7 cut(s) 1106, 1580, 1940, 2899, 4608, 5217, 5450
BsmBI CGTCTC 1 cut(s) 597
BsmFI GGGAC 4 cut(s) 23, 877, 2549, 5087
BsmI GAATGC 4 cut(s) 2644, 3614, 3894, 5473
BsnI GGCC 7 cut(s) 207, 1815, 2276, 2282, 2610, 3251, 4929
Bso31I GGTCTC 3 cut(s) 499, 1799, 4724
BsoBI CYCGRG 4 cut(s) 33, 977, 3070, 4757
Bsp119I TTCGAA 1 cut(s) 4129
Bsp1286I GDGCHC 8 cut(s) 201, 261, 978, 984, 1588, 2421, 2893, 3684
Bsp1407I TGTACA 1 cut(s) 3453
Bsp1720I GCTNAGC 1 cut(s) 4986
Bsp19I CCATGG 4 cut(s) 2277, 2382, 4830, 5445
Bsp68I TCGCGA 2 cut(s) 195, 1914
BspANI GGCC 7 cut(s) 207, 1815, 2276, 2282, 2610, 3251, 4929
BspDI ATCGAT 2 cut(s) 818, 1409
BspFNI CGCG 5 cut(s) 195, 246, 269, 1914, 2531
BspHI TCATGA 1 cut(s) 1360
BspMAI CTGCAG 2 cut(s) 4640, 4923
BspMI ACCTGC 2 cut(s) 3908, 4819
BspOI GCTAGC 1 cut(s) 2882
BspQI GCTCTTC 2 cut(s) 341, 2856
BspT104I TTCGAA 1 cut(s) 4129
BspT107I GGYRCC 2 cut(s) 2888, 5389
BspTI CTTAAG 1 cut(s) 5522
BspTNI GGTCTC 3 cut(s) 499, 1799, 4724
BsrDI GCAATG 6 cut(s) 358, 627, 1494, 2157, 2871, 3456
BsrFI RCCGGY 1 cut(s) 4947
BsrGI TGTACA 1 cut(s) 3453
BssAI RCCGGY 1 cut(s) 4947
BssNAI GTATAC 2 cut(s) 1401, 3827
BssNI GRCGYC 2 cut(s) 138, 590
BssT1I CCWWGG 7 cut(s) 2277, 2382, 2593, 4146, 4331, 4830, 5445
Bst1107I GTATAC 2 cut(s) 1401, 3827
Bst2UI CCWGG 9 cut(s) 1248, 1378, 1801, 2229, 2339, 2544, 3166, 4267, 4609
Bst6I CTCTTC 7 cut(s) 308, 341, 2324, 2838, 2856, 4654, 5298
BstACI GRCGYC 2 cut(s) 138, 590
BstAFI CTTAAG 1 cut(s) 5522
BstAPI GCANNNNNTGC 1 cut(s) 1840
BstAUI TGTACA 1 cut(s) 3453
BstBAI YACGTR 2 cut(s) 923, 3260
BstBI TTCGAA 1 cut(s) 4129
BstC8I GCNNGC 8 cut(s) 197, 251, 1599, 1787, 1972, 2880, 3760, 3798
BstDSI CCRYGG 6 cut(s) 244, 2277, 2382, 4830, 5181, 5445
BstFNI CGCG 5 cut(s) 195, 246, 269, 1914, 2531
BstHHI GCGC 4 cut(s) 271, 2730, 2878, 3469
BstNI CCWGG 9 cut(s) 1248, 1378, 1801, 2229, 2339, 2544, 3166, 4267, 4609
BstNSI RCATGY 7 cut(s) 1136, 1772, 2087, 3800, 4881, 5387, 5419
BstSFI CTRYAG 5 cut(s) 1539, 2100, 3579, 4636, 4919
BstSLI GKGCMC 1 cut(s) 2893
BstSNI TACGTA 1 cut(s) 3260
BstUI CGCG 5 cut(s) 195, 246, 269, 1914, 2531
BstV2I GAAGAC 4 cut(s) 644, 3758, 5035, 5331
BstX2I RGATCY 8 cut(s) 278, 1153, 1250, 1373, 1570, 2858, 3154, 3927
BstXI CCANNNNNNTGG 3 cut(s) 1377, 3610, 5386
BstYI RGATCY 8 cut(s) 278, 1153, 1250, 1373, 1570, 2858, 3154, 3927
BstZ17I GTATAC 2 cut(s) 1401, 3827
Bsu15I ATCGAT 2 cut(s) 818, 1409
Bsu36I CCTNAGG 1 cut(s) 2496
BsuI GTATCC 3 cut(s) 528, 1239, 3665
BsuRI GGCC 7 cut(s) 207, 1815, 2276, 2282, 2610, 3251, 4929
BsuTUI ATCGAT 2 cut(s) 818, 1409
BtgI CCRYGG 6 cut(s) 244, 2277, 2382, 4830, 5181, 5445
BtgZI GCGATG 2 cut(s) 432, 1929
BtrI CACGTC 3 cut(s) 134, 4370, 5077
BtsI GCAGTG 1 cut(s) 1533
BtsIMutI CAGTG 9 cut(s) 155, 1533, 1860, 2097, 2249, 2367, 3368, 4677, 5138
BtuMI TCGCGA 2 cut(s) 195, 1914
BveI ACCTGC 2 cut(s) 3908, 4819
Cac8I GCNNGC 8 cut(s) 197, 251, 1599, 1787, 1972, 2880, 3760, 3798
CaiI CAGNNNCTG 1 cut(s) 3923
CciI TCATGA 1 cut(s) 1360
CfoI GCGC 4 cut(s) 271, 2730, 2878, 3469
Cfr10I RCCGGY 1 cut(s) 4947
Cfr13I GGNCC 9 cut(s) 37, 328, 1948, 2281, 2608, 2965, 4001, 5248, 5376
Cfr42I CCGCGG 1 cut(s) 247
Cfr9I CCCGGG 1 cut(s) 33
ClaI ATCGAT 2 cut(s) 818, 1409
CseI GACGC 4 cut(s) 146, 579, 2165, 2537
CsiI ACCWGGT 1 cut(s) 2542
Csp6I GTAC 7 cut(s) 1367, 2372, 3192, 3351, 3454, 4536, 4762
CviQI GTAC 7 cut(s) 1367, 2372, 3192, 3351, 3454, 4536, 4762
DraI TTTAAA 1 cut(s) 2820
DriI GACNNNNNGTC 3 cut(s) 648, 1870, 5503
EaeI YGGCCR 1 cut(s) 1813
Eam1104I CTCTTC 7 cut(s) 308, 341, 2324, 2838, 2856, 4654, 5298
Eam1105I GACNNNNNGTC 3 cut(s) 648, 1870, 5503
EarI CTCTTC 7 cut(s) 308, 341, 2324, 2838, 2856, 4654, 5298
EciI GGCGGA 2 cut(s) 85, 230
Ecl136II GAGCTC 5 cut(s) 199, 259, 976, 1586, 3682
Eco105I TACGTA 1 cut(s) 3260
Eco130I CCWWGG 7 cut(s) 2277, 2382, 2593, 4146, 4331, 4830, 5445
Eco147I AGGCCT 1 cut(s) 3251
Eco24I GRGCYC 5 cut(s) 201, 261, 978, 1588, 3684
Eco31I GGTCTC 3 cut(s) 499, 1799, 4724
Eco47I GGWCC 7 cut(s) 37, 328, 1948, 2965, 4001, 5248, 5376
Eco53kI GAGCTC 5 cut(s) 199, 259, 976, 1586, 3682
Eco57I CTGAAG 7 cut(s) 1727, 2181, 2193, 2496, 2651, 4475, 4635
Eco81I CCTNAGG 1 cut(s) 2496
Eco88I CYCGRG 4 cut(s) 33, 977, 3070, 4757
EcoICRI GAGCTC 5 cut(s) 199, 259, 976, 1586, 3682
EcoO109I RGGNCCY 3 cut(s) 37, 2608, 4001
EcoRI GAATTC 3 cut(s) 550, 1291, 4434
EcoRII CCWGG 9 cut(s) 1246, 1376, 1799, 2227, 2337, 2542, 3164, 4265, 4607
EcoT14I CCWWGG 7 cut(s) 2277, 2382, 2593, 4146, 4331, 4830, 5445
EcoT22I ATGCAT 6 cut(s) 727, 1518, 1892, 3752, 4787, 5475
EcoT38I GRGCYC 5 cut(s) 201, 261, 978, 1588, 3684
ErhI CCWWGG 7 cut(s) 2277, 2382, 2593, 4146, 4331, 4830, 5445
Esp3I CGTCTC 1 cut(s) 597
FaqI GGGAC 4 cut(s) 23, 877, 2549, 5087
FauI CCCGC 2 cut(s) 178, 1452
FauNDI CATATG 4 cut(s) 2201, 2413, 4672, 5069
FbaI TGATCA 3 cut(s) 3043, 4084, 4486
FblI GTMKAC 6 cut(s) 180, 1053, 1111, 1400, 2103, 3826
FriOI GRGCYC 5 cut(s) 201, 261, 978, 1588, 3684
GlaI GCGC 4 cut(s) 270, 2729, 2877, 3468
GsaI CCCAGC 3 cut(s) 3465, 3927, 5027
GsuI CTGGAG 8 cut(s) 44, 245, 275, 829, 2255, 2360, 4585, 5239
HaeIII GGCC 7 cut(s) 207, 1815, 2276, 2282, 2610, 3251, 4929
HapII CCGG 7 cut(s) 34, 129, 331, 753, 4948, 5211, 5393
HgaI GACGC 4 cut(s) 146, 579, 2165, 2537
HhaI GCGC 4 cut(s) 271, 2730, 2878, 3469
Hin1I GRCGYC 2 cut(s) 138, 590
Hin6I GCGC 4 cut(s) 269, 2728, 2876, 3467
HinP1I GCGC 4 cut(s) 269, 2728, 2876, 3467
HincII GTYRAC 5 cut(s) 181, 642, 1417, 3904, 4156
HindII GTYRAC 5 cut(s) 181, 642, 1417, 3904, 4156
HindIII AAGCTT 1 cut(s) 5525
HpaI GTTAAC 1 cut(s) 3904
HpaII CCGG 7 cut(s) 34, 129, 331, 753, 4948, 5211, 5393
HphI GGTGA 6 cut(s) 778, 2512, 3097, 3248, 3508, 4921
Hpy99I CGWCG 4 cut(s) 179, 182, 215, 221
HpyCH4IV ACGT 7 cut(s) 133, 660, 922, 1041, 3259, 4369, 5076
HpySE526I ACGT 7 cut(s) 133, 660, 922, 1041, 3259, 4369, 5076
Hsp92I GRCGYC 2 cut(s) 138, 590
HspAI GCGC 4 cut(s) 269, 2728, 2876, 3467
KflI GGGWCCC 1 cut(s) 37
Ksp22I TGATCA 3 cut(s) 3043, 4084, 4486
KspAI GTTAAC 1 cut(s) 3904
KspI CCGCGG 1 cut(s) 247
LguI GCTCTTC 2 cut(s) 341, 2856
MabI ACCWGGT 1 cut(s) 2542
MaeII ACGT 7 cut(s) 133, 660, 922, 1041, 3259, 4369, 5076
MfeI CAATTG 3 cut(s) 852, 4091, 5371
MflI RGATCY 8 cut(s) 278, 1153, 1250, 1373, 1570, 2858, 3154, 3927
MhlI GDGCHC 8 cut(s) 201, 261, 978, 984, 1588, 2421, 2893, 3684
MlyI GAGTC 7 cut(s) 107, 113, 389, 1444, 2833, 3197, 3411
MmeI TCCRAC 1 cut(s) 141
Mph1103I ATGCAT 6 cut(s) 727, 1518, 1892, 3752, 4787, 5475
MroXI GAANNNNTTC 4 cut(s) 651, 2391, 2720, 4622
MslI CAYNNNNRTG 7 cut(s) 1895, 2031, 3281, 3608, 4400, 4790, 5384
MspA1I CMGCKG 4 cut(s) 246, 2936, 2999, 5150
MspCI CTTAAG 1 cut(s) 5522
MspI CCGG 7 cut(s) 34, 129, 331, 753, 4948, 5211, 5393
MunI CAATTG 3 cut(s) 852, 4091, 5371
Mva1269I GAATGC 4 cut(s) 2644, 3614, 3894, 5473
MvaI CCWGG 9 cut(s) 1248, 1378, 1801, 2229, 2339, 2544, 3166, 4267, 4609
MvnI CGCG 5 cut(s) 195, 246, 269, 1914, 2531
NciI CCSGG 4 cut(s) 34, 35, 129, 5394
NcoI CCATGG 4 cut(s) 2277, 2382, 4830, 5445
NdeI CATATG 4 cut(s) 2201, 2413, 4672, 5069
NheI GCTAGC 1 cut(s) 2878
NmeAIII GCCGAG 1 cut(s) 1946
NmuCI GTSAC 7 cut(s) 134, 2525, 2810, 3236, 4393, 5278, 5551
NruI TCGCGA 2 cut(s) 195, 1914
NsiI ATGCAT 6 cut(s) 727, 1518, 1892, 3752, 4787, 5475
NspI RCATGY 7 cut(s) 1136, 1772, 2087, 3800, 4881, 5387, 5419
NspV TTCGAA 1 cut(s) 4129
OliI CACNNNNGTG 3 cut(s) 2031, 3281, 5384
PaeI GCATGC 1 cut(s) 3800
PaeR7I CTCGAG 3 cut(s) 977, 3070, 4757
PagI TCATGA 1 cut(s) 1360
PaqCI CACCTGC 1 cut(s) 3908
PceI AGGCCT 1 cut(s) 3251
PciI ACATGT 2 cut(s) 1132, 5383
PciSI GCTCTTC 2 cut(s) 341, 2856
PcsI WCGNNNNNNNCGW 2 cut(s) 279, 587
PctI GAATGC 4 cut(s) 2644, 3614, 3894, 5473
PdmI GAANNNNTTC 4 cut(s) 651, 2391, 2720, 4622
PflFI GACNNNGTC 1 cut(s) 3242
PleI GAGTC 7 cut(s) 107, 113, 388, 1444, 2832, 3197, 3411
PpsI GAGTC 7 cut(s) 107, 113, 388, 1444, 2832, 3197, 3411
Ppu21I YACGTR 2 cut(s) 923, 3260
PpuMI RGGWCCY 2 cut(s) 37, 4001
PscI ACATGT 2 cut(s) 1132, 5383
Psp124BI GAGCTC 5 cut(s) 201, 261, 978, 1588, 3684
Psp5II RGGWCCY 2 cut(s) 37, 4001
Psp6I CCWGG 9 cut(s) 1246, 1376, 1799, 2227, 2337, 2542, 3164, 4265, 4607
PspFI CCCAGC 3 cut(s) 3461, 3923, 5023
PspGI CCWGG 9 cut(s) 1246, 1376, 1799, 2227, 2337, 2542, 3164, 4265, 4607
PspPI GGNCC 9 cut(s) 37, 328, 1948, 2281, 2608, 2965, 4001, 5248, 5376
PspPPI RGGWCCY 2 cut(s) 37, 4001
PspXI VCTCGAGB 1 cut(s) 977
PsrI GAACNNNNNNTAC 2 cut(s) 3495, 3527
PstI CTGCAG 2 cut(s) 4640, 4923
PstNI CAGNNNCTG 1 cut(s) 3923
PsuI RGATCY 8 cut(s) 278, 1153, 1250, 1373, 1570, 2858, 3154, 3927
PsyI GACNNNGTC 1 cut(s) 3242
PvuII CAGCTG 3 cut(s) 2936, 2999, 5150
RruI TCGCGA 2 cut(s) 195, 1914
RsaI GTAC 7 cut(s) 1368, 2373, 3193, 3352, 3455, 4537, 4763
RsaNI GTAC 7 cut(s) 1367, 2372, 3192, 3351, 3454, 4536, 4762
RseI CAYNNNNRTG 7 cut(s) 1895, 2031, 3281, 3608, 4400, 4790, 5384
SacI GAGCTC 5 cut(s) 201, 261, 978, 1588, 3684
SacII CCGCGG 1 cut(s) 247
SalI GTCGAC 1 cut(s) 179
SapI GCTCTTC 2 cut(s) 341, 2856
Sau96I GGNCC 9 cut(s) 37, 328, 1948, 2281, 2608, 2965, 4001, 5248, 5376
SbfI CCTGCAGG 1 cut(s) 4923
SchI GAGTC 7 cut(s) 107, 113, 389, 1444, 2833, 3197, 3411
SdaI CCTGCAGG 1 cut(s) 4923
SduI GDGCHC 8 cut(s) 201, 261, 978, 984, 1588, 2421, 2893, 3684
SexAI ACCWGGT 1 cut(s) 2542
SfcI CTRYAG 5 cut(s) 1539, 2100, 3579, 4636, 4919
Sfr274I CTCGAG 3 cut(s) 977, 3070, 4757
Sfr303I CCGCGG 1 cut(s) 247
SfuI TTCGAA 1 cut(s) 4129
SgrBI CCGCGG 1 cut(s) 247
SinI GGWCC 7 cut(s) 37, 328, 1948, 2965, 4001, 5248, 5376
SlaI CTCGAG 3 cut(s) 977, 3070, 4757
SmaI CCCGGG 1 cut(s) 35
SmiMI CAYNNNNRTG 7 cut(s) 1895, 2031, 3281, 3608, 4400, 4790, 5384
SmlI CTYRAG 9 cut(s) 502, 977, 1452, 3022, 3070, 4613, 4757, 5510, 5522
SmoI CTYRAG 9 cut(s) 502, 977, 1452, 3022, 3070, 4613, 4757, 5510, 5522
SnaBI TACGTA 1 cut(s) 3260
SphI GCATGC 1 cut(s) 3800
Sse8387I CCTGCAGG 1 cut(s) 4923
SseBI AGGCCT 1 cut(s) 3251
SspI AATATT 1 cut(s) 5046
SstI GAGCTC 5 cut(s) 201, 261, 978, 1588, 3684
StuI AGGCCT 1 cut(s) 3251
StyI CCWWGG 7 cut(s) 2277, 2382, 2593, 4146, 4331, 4830, 5445
TaiI ACGT 7 cut(s) 136, 663, 925, 1044, 3262, 4372, 5079
TatI WGTACW 5 cut(s) 2371, 3350, 3453, 4535, 4761
TauI GCSGC 5 cut(s) 207, 246, 249, 1148, 5410
TscAI CASTG 9 cut(s) 155, 1540, 1867, 2104, 2249, 2374, 3368, 4684, 5138
TseFI GTSAC 7 cut(s) 134, 2525, 2810, 3236, 4393, 5278, 5551
Tsp45I GTSAC 7 cut(s) 134, 2525, 2810, 3236, 4393, 5278, 5551
TspGWI ACGGA 8 cut(s) 234, 560, 2901, 3209, 4412, 4415, 4714, 5130
TspMI CCCGGG 1 cut(s) 33
TspRI CASTG 9 cut(s) 155, 1540, 1867, 2104, 2249, 2374, 3368, 4684, 5138
Tth111I GACNNNGTC 1 cut(s) 3242
Vha464I CTTAAG 1 cut(s) 5522
VpaK11BI GGWCC 7 cut(s) 37, 328, 1948, 2965, 4001, 5248, 5376
XapI RAATTY 9 cut(s) 550, 691, 1291, 2945, 4434, 4481, 4912, 4971, 5364
XbaI TCTAGA 1 cut(s) 2675
XceI RCATGY 7 cut(s) 1136, 1772, 2087, 3800, 4881, 5387, 5419
XcmI CCANNNNNNNNNTGG 4 cut(s) 902, 1564, 3665, 4347
XhoI CTCGAG 3 cut(s) 977, 3070, 4757
XmaI CCCGGG 1 cut(s) 33
XmaJI CCTAGG 1 cut(s) 4331
XmiI GTMKAC 6 cut(s) 180, 1053, 1111, 1400, 2103, 3826
XmnI GAANNNNTTC 4 cut(s) 651, 2391, 2720, 4622
Zsp2I ATGCAT 6 cut(s) 727, 1518, 1892, 3752, 4787, 5475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.