pycom111g02220

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0001894
Physical Location & Seq
Forward (+)
1335130 .. 1336536
1407 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g02220.2

Sequence Viewer

Length: 777 bp
ATGGAGGTGCTCTTGTCCTTGATGCCTATGATGCAGTTGGCTGACACAGTAGGAGTAGGTCAGTGGCATTCTGTAGAGTGGTCGGAAGCAGTCAGATGCTTTGGCAAGGCTCGGAAAAGTTGGTTGTTGAAGTTCCTGCAGGTTACCCAGGAGGACTTGCAGCAAAGAGATGGACAGATTATTGAAGTTCTGAAAAAGATTCAAACCAAAGCTAAGCTCGTTAGAGTTGGCTCCATCCCACTGACTGAGCTGGGAAAGCTAAAGCCTTGGATATTGAACACTCGATCAAATGTTATATGGGATGTGCTCGTTGAAGTTGTAGCAGCTTTGCAACATGCAGATGGAAGTGTCAAAAGACAGTGGCTTGTTGATGCAGTTGAAATCAGCTGTGTATCAAGTTATCCATCCACGGCGCTACAATTTCTTGGGTTGCTGTCCGGTAGCTGGAGCAAATATATGCCGCTTCTGATTCTGGACCAACGTGCTGTGCTGAGTGATTTACCAGTCACTCTTTCCTCCCTTCTTTCAGACTCTAGTTGGGAAGGAGTTGTAGAACTCGCCGTCCCTTCTCTTTTTGCATCGACAGAACGCATTTACAATTGGGCTACACATATCATGCGCGGTGAGGATATCCCGCCTGACATGCAAACTATTGACAAAAGTGAGAGTTCCATGGCTGTTTTCCTATCAAGAGTGATGCATGGTACCTGTGTATCTTTGAAGGATTACCTGCCTCTCGAGAAGCAACTTAAGCTTGCAAATATGGCGGTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3730

Protein Analysis

259

Amino Acids

28.84

Weight (kDa)

6.11

Isoelectric Point (pI)

39.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 130, 738
Acc65I GGTACC 1 cut(s) 704
AccB1I GGYRCC 1 cut(s) 704
AccII CGCG 1 cut(s) 621
AciI CCGC 4 cut(s) 461, 621, 635, 767
AfaI GTAC 1 cut(s) 706
AfiI CCNNNNNNNGG 1 cut(s) 444
AflII CTTAAG 1 cut(s) 749
AgsI TTSAA 7 cut(s) 130, 185, 203, 277, 314, 380, 721
AjnI CCWGG 1 cut(s) 147
AloI GAACNNNNNNTCC 2 cut(s) 546, 578
AluBI AGCT 8 cut(s) 212, 217, 250, 259, 326, 387, 444, 754
AluI AGCT 8 cut(s) 212, 217, 250, 259, 326, 387, 444, 754
Alw21I GWGCWC 2 cut(s) 12, 309
Ama87I CYCGRG 1 cut(s) 737
ApeKI GCWGC 2 cut(s) 160, 323
Asp718I GGTACC 1 cut(s) 704
AspLEI GCGC 2 cut(s) 415, 621
AspS9I GGNCC 1 cut(s) 475
AsuHPI GGTGA 1 cut(s) 635
AvaI CYCGRG 1 cut(s) 737
AvaII GGWCC 1 cut(s) 475
BaeI ACNNNNGTAYC 4 cut(s) 696, 696, 729, 729
BanI GGYRCC 1 cut(s) 704
Bbv12I GWGCWC 2 cut(s) 12, 309
BbvI GCAGC 2 cut(s) 172, 335
BccI CCATC 4 cut(s) 164, 242, 335, 412
BceAI ACGGC 2 cut(s) 426, 545
BciT130I CCWGG 1 cut(s) 149
BfaI CTAG 1 cut(s) 534
BfmI CTRYAG 2 cut(s) 72, 137
BfoI RGCGCY 1 cut(s) 416
BfrI CTTAAG 1 cut(s) 749
BfuAI ACCTGC 2 cut(s) 130, 738
BisI GCNGC 3 cut(s) 161, 324, 461
BlpI GCTNAGC 1 cut(s) 213
BlsI GCNGC 3 cut(s) 162, 325, 462
Bme1390I CCNGG 1 cut(s) 149
Bme18I GGWCC 1 cut(s) 475
BmeT110I CYCGRG 1 cut(s) 737
BmgT120I GGNCC 1 cut(s) 475
BmiI GGNNCC 2 cut(s) 232, 706
BmrFI CCNGG 1 cut(s) 149
BmsI GCATC 6 cut(s) 12, 21, 86, 361, 587, 687
BpmI CTGGAG 1 cut(s) 466
Bpu1102I GCTNAGC 1 cut(s) 213
BsaJI CCNNGG 4 cut(s) 147, 266, 408, 672
BsaWI WCCGGW 1 cut(s) 437
Bsc4I CCNNNNNNNGG 1 cut(s) 444
Bse1I ACTGG 1 cut(s) 503
BseBI CCWGG 1 cut(s) 149
BseDI CCNNGG 4 cut(s) 147, 266, 408, 672
BseGI GGATG 3 cut(s) 234, 307, 404
BseLI CCNNNNNNNGG 1 cut(s) 444
BseMII CTCAG 2 cut(s) 237, 482
BseNI ACTGG 1 cut(s) 503
BseXI GCAGC 2 cut(s) 172, 335
BseYI CCCAGC 1 cut(s) 250
Bsh1236I CGCG 1 cut(s) 621
BshNI GGYRCC 1 cut(s) 704
BsiHKAI GWGCWC 2 cut(s) 12, 309
BsiHKCI CYCGRG 1 cut(s) 737
BsiSI CCGG 1 cut(s) 438
BslFI GGGAC 1 cut(s) 548
BslI CCNNNNNNNGG 1 cut(s) 444
BsmFI GGGAC 1 cut(s) 548
BsmI GAATGC 1 cut(s) 67
BsoBI CYCGRG 1 cut(s) 737
Bsp1286I GDGCHC 2 cut(s) 12, 309
Bsp143I GATC 1 cut(s) 284
Bsp1720I GCTNAGC 1 cut(s) 213
Bsp19I CCATGG 1 cut(s) 672
BspACI CCGC 4 cut(s) 461, 621, 635, 767
BspCNI CTCAG 2 cut(s) 238, 483
BspFNI CGCG 1 cut(s) 621
BspLI GGNNCC 2 cut(s) 232, 706
BspMAI CTGCAG 1 cut(s) 141
BspMI ACCTGC 2 cut(s) 130, 738
BspT107I GGYRCC 1 cut(s) 704
BspTI CTTAAG 1 cut(s) 749
BsrI ACTGG 1 cut(s) 503
BssECI CCNNGG 4 cut(s) 147, 266, 408, 672
BssMI GATC 1 cut(s) 284
BssT1I CCWWGG 2 cut(s) 266, 672
Bst2UI CCWGG 1 cut(s) 149
Bst4CI ACNGT 2 cut(s) 49, 360
BstAFI CTTAAG 1 cut(s) 749
BstC8I GCNNGC 1 cut(s) 756
BstDEI CTNAG 3 cut(s) 213, 246, 491
BstDSI CCRYGG 2 cut(s) 408, 672
BstEII GGTNACC 1 cut(s) 142
BstF5I GGATG 3 cut(s) 234, 307, 404
BstFNI CGCG 1 cut(s) 621
BstH2I RGCGCY 1 cut(s) 416
BstHHI GCGC 2 cut(s) 415, 621
BstKTI GATC 1 cut(s) 287
BstMBI GATC 1 cut(s) 284
BstMWI GCNNNNNNNGC 5 cut(s) 31, 256, 643, 751, 764
BstNI CCWGG 1 cut(s) 149
BstNSI RCATGY 2 cut(s) 338, 646
BstPI GGTNACC 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 147
BstSFI CTRYAG 2 cut(s) 72, 137
BstUI CGCG 1 cut(s) 621
BstV1I GCAGC 2 cut(s) 172, 335
BtgI CCRYGG 2 cut(s) 408, 672
BtsCI GGATG 3 cut(s) 234, 307, 404
BtsIMutI CAGTG 3 cut(s) 68, 239, 365
BveI ACCTGC 2 cut(s) 130, 738
Cac8I GCNNGC 1 cut(s) 756
CfoI GCGC 2 cut(s) 415, 621
Cfr13I GGNCC 1 cut(s) 475
Csp6I GTAC 1 cut(s) 705
CviAII CATG 5 cut(s) 335, 616, 643, 673, 701
CviQI GTAC 1 cut(s) 705
DdeI CTNAG 3 cut(s) 213, 246, 491
DpnI GATC 1 cut(s) 286
DpnII GATC 1 cut(s) 284
Eco130I CCWWGG 2 cut(s) 266, 672
Eco32I GATATC 1 cut(s) 631
Eco47I GGWCC 1 cut(s) 475
Eco88I CYCGRG 1 cut(s) 737
Eco91I GGTNACC 1 cut(s) 142
EcoO65I GGTNACC 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 147
EcoRV GATATC 1 cut(s) 631
EcoT14I CCWWGG 2 cut(s) 266, 672
EcoT22I ATGCAT 1 cut(s) 702
ErhI CCWWGG 2 cut(s) 266, 672
FaeI CATG 5 cut(s) 338, 619, 646, 676, 704
FaqI GGGAC 1 cut(s) 548
FatI CATG 5 cut(s) 334, 615, 642, 672, 700
FauI CCCGC 1 cut(s) 642
Fnu4HI GCNGC 3 cut(s) 161, 324, 461
FokI GGATG 3 cut(s) 221, 314, 391
Fsp4HI GCNGC 3 cut(s) 161, 324, 461
FspBI CTAG 1 cut(s) 534
GlaI GCGC 2 cut(s) 414, 620
GluI GCNGC 3 cut(s) 161, 324, 461
GsaI CCCAGC 1 cut(s) 254
GsuI CTGGAG 1 cut(s) 466
HaeII RGCGCY 1 cut(s) 416
HapII CCGG 1 cut(s) 438
HhaI GCGC 2 cut(s) 415, 621
Hin1II CATG 5 cut(s) 338, 619, 646, 676, 704
Hin6I GCGC 2 cut(s) 413, 619
HinP1I GCGC 2 cut(s) 413, 619
HindIII AAGCTT 1 cut(s) 752
HinfI GANTC 3 cut(s) 199, 469, 530
HpaII CCGG 1 cut(s) 438
HphI GGTGA 1 cut(s) 635
Hpy188I TCNGA 6 cut(s) 85, 95, 114, 192, 468, 529
Hpy188III TCNNGA 4 cut(s) 473, 690, 737, 739
HpyAV CCTTC 4 cut(s) 530, 536, 576, 715
HpyCH4III ACNGT 2 cut(s) 49, 360
HpyCH4IV ACGT 1 cut(s) 481
HpyF10VI GCNNNNNNNGC 5 cut(s) 31, 256, 643, 751, 764
HpyF3I CTNAG 3 cut(s) 213, 246, 491
HpySE526I ACGT 1 cut(s) 481
Hsp92II CATG 5 cut(s) 338, 619, 646, 676, 704
HspAI GCGC 2 cut(s) 413, 619
KpnI GGTACC 1 cut(s) 708
Kzo9I GATC 1 cut(s) 284
LmnI GCTCC 2 cut(s) 236, 447
Lsp1109I GCAGC 2 cut(s) 172, 335
LweI GCATC 6 cut(s) 12, 21, 86, 361, 587, 687
MaeI CTAG 1 cut(s) 534
MaeII ACGT 1 cut(s) 481
MaeIII GTNAC 2 cut(s) 142, 505
MalI GATC 1 cut(s) 286
MboI GATC 1 cut(s) 284
MfeI CAATTG 1 cut(s) 598
MhlI GDGCHC 2 cut(s) 12, 309
MluCI AATT 2 cut(s) 419, 598
MlyI GAGTC 1 cut(s) 524
MmeI TCCRAC 1 cut(s) 63
MnlI CCTC 4 cut(s) 145, 526, 619, 744
Mph1103I ATGCAT 1 cut(s) 702
MseI TTAA 1 cut(s) 750
MslI CAYNNNNRTG 1 cut(s) 339
MspA1I CMGCKG 1 cut(s) 387
MspCI CTTAAG 1 cut(s) 749
MspI CCGG 1 cut(s) 438
MspR9I CCNGG 1 cut(s) 149
MunI CAATTG 1 cut(s) 598
Mva1269I GAATGC 1 cut(s) 67
MvaI CCWGG 1 cut(s) 149
MvnI CGCG 1 cut(s) 621
MwoI GCNNNNNNNGC 5 cut(s) 31, 256, 643, 751, 764
NcoI CCATGG 1 cut(s) 672
NdeII GATC 1 cut(s) 284
NlaIII CATG 5 cut(s) 338, 619, 646, 676, 704
NlaIV GGNNCC 2 cut(s) 232, 706
NmuCI GTSAC 1 cut(s) 505
NsiI ATGCAT 1 cut(s) 702
NspI RCATGY 2 cut(s) 338, 646
PaeR7I CTCGAG 1 cut(s) 737
PctI GAATGC 1 cut(s) 67
PfeI GAWTC 2 cut(s) 199, 469
PkrI GCNGC 3 cut(s) 162, 325, 462
PleI GAGTC 1 cut(s) 524
PpsI GAGTC 1 cut(s) 524
Psp6I CCWGG 1 cut(s) 147
PspEI GGTNACC 1 cut(s) 142
PspFI CCCAGC 1 cut(s) 250
PspGI CCWGG 1 cut(s) 147
PspN4I GGNNCC 2 cut(s) 232, 706
PspPI GGNCC 1 cut(s) 475
PstI CTGCAG 1 cut(s) 141
PvuII CAGCTG 1 cut(s) 387
RsaI GTAC 1 cut(s) 706
RsaNI GTAC 1 cut(s) 705
RseI CAYNNNNRTG 1 cut(s) 339
SaqAI TTAA 1 cut(s) 750
SatI GCNGC 3 cut(s) 161, 324, 461
Sau3AI GATC 1 cut(s) 284
Sau96I GGNCC 1 cut(s) 475
SbfI CCTGCAGG 1 cut(s) 141
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 1 cut(s) 149
SdaI CCTGCAGG 1 cut(s) 141
SduI GDGCHC 2 cut(s) 12, 309
SfaNI GCATC 6 cut(s) 12, 21, 86, 361, 587, 687
SfcI CTRYAG 2 cut(s) 72, 137
Sfr274I CTCGAG 1 cut(s) 737
SinI GGWCC 1 cut(s) 475
SlaI CTCGAG 1 cut(s) 737
SmiMI CAYNNNNRTG 1 cut(s) 339
SmlI CTYRAG 2 cut(s) 737, 749
SmoI CTYRAG 2 cut(s) 737, 749
Sse8387I CCTGCAGG 1 cut(s) 141
Sse9I AATT 2 cut(s) 419, 598
SsiI CCGC 4 cut(s) 461, 621, 635, 767
SspMI CTAG 1 cut(s) 534
StyD4I CCNGG 1 cut(s) 147
StyI CCWWGG 2 cut(s) 266, 672
TaaI ACNGT 2 cut(s) 49, 360
TaiI ACGT 1 cut(s) 484
TaqI TCGA 3 cut(s) 283, 581, 738
TasI AATT 2 cut(s) 419, 598
TauI GCSGC 1 cut(s) 463
TfiI GAWTC 2 cut(s) 199, 469
Tru1I TTAA 1 cut(s) 750
Tru9I TTAA 1 cut(s) 750
TscAI CASTG 3 cut(s) 68, 246, 365
TseFI GTSAC 1 cut(s) 505
TseI GCWGC 2 cut(s) 160, 323
Tsp45I GTSAC 1 cut(s) 505
TspRI CASTG 3 cut(s) 68, 246, 365
Vha464I CTTAAG 1 cut(s) 749
VpaK11BI GGWCC 1 cut(s) 475
XceI RCATGY 2 cut(s) 338, 646
XhoI CTCGAG 1 cut(s) 737
XspI CTAG 1 cut(s) 534
Zsp2I ATGCAT 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.