FvH4_6g53510

B2 protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002574
Physical Location & Seq
Forward (+)
39207775 .. 39211736
3962 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g53510.t3

Sequence Viewer

Length: 903 bp
ATGGAGAGCATCCACACCTATTGGCAATTGGGTGATGAGCTTCGTGGACAGTCGAAAGTTGCAGAGGATCACAAATGGTTAATGGCTGCTTCCAAATTGGCTGAGCAAACGAGGTCAAAAGGAGAGCGCATGAATAACCTTGATCTTTCAAAGGGCCCAGCTGAAACAAGAGCAAGGGACAAATTCGGTTTCCAGGAAGATAACAAATTCGAGAGCCTTAACTTTAACATGTTGAACTTGGACTCAAAAGTAAATGAAAATTTGAGCAAGAGTTCCATTAGGAATGGGATTTACAACATGAATGCAGTTTACCAGAAAAACAGTGCAAGCTTTGTGGGAAATATGACTGGTAACAAGTATGGTGTCAATAATCTCAGCAACAAGGAAACCAACAACATCAGTAACAACAACAACAACAACGAGGCTGGGAATGTTGTTGAGAAAAGGTTCAAGACCTTGCCTGCAACTGAGACACTTCCCCGAAATGAGGTGCTTGGAGGCTACATCTTTGTATGCAACAATGACACAATGCAGGAAGATTTGAAGCGACAACTATTCGGTTTACCTCCAAGGTACAGGGATTCTGTTCGGGCAATCACACCCGGCTTGCCTCTGTTTCTCTACAATTACACAACACACCAACTGCATGGCATTTTTGAGGCATCAAGCTTTGGTGGTTCAAACATTGATCCAACTGCATGGGAAGACAAGAAATGTAAAGGGGAGTCAAGGTTTCCTGCGCAGGTGAGGATCCGTGTTAGAAAAATCTGCAAGGCTCTGGAAGAAGATTCCTTCAGGCCAGTCCTGCATCACTATGATGGTCCAAAGTTCCGTCTTGAGTTGTCAGTTCCTGAGACCCTGGATCTATTGGACCTATGTGAGCAAGCCGGCTCTGCAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

301

Amino Acids

33.99

Weight (kDa)

7.67

Isoelectric Point (pI)

37.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 166 - 222 3.4e-19 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 733
Acc16I TGCGCA 1 cut(s) 741
Acc36I ACCTGC 1 cut(s) 733
AclWI GGATC 5 cut(s) 75, 683, 745, 758, 870
AcsI RAATTY 3 cut(s) 182, 206, 259
AcuI CTGAAG 1 cut(s) 778
AfaI GTAC 1 cut(s) 575
AfiI CCNNNNNNNGG 1 cut(s) 487
AflIII ACRYGT 1 cut(s) 228
AgsI TTSAA 5 cut(s) 150, 235, 451, 544, 681
AjnI CCWGG 2 cut(s) 192, 858
AluBI AGCT 4 cut(s) 40, 161, 330, 669
AluI AGCT 4 cut(s) 40, 161, 330, 669
Alw26I GTCTC 2 cut(s) 464, 848
AlwI GGATC 5 cut(s) 75, 683, 745, 758, 870
AlwNI CAGNNNCTG 1 cut(s) 851
AoxI GGCC 2 cut(s) 154, 797
ApaI GGGCCC 1 cut(s) 158
ApeKI GCWGC 2 cut(s) 86, 896
ApoI RAATTY 3 cut(s) 182, 206, 259
Asp700I GAANNNNTTC 1 cut(s) 446
AspLEI GCGC 2 cut(s) 129, 742
AspS9I GGNCC 4 cut(s) 154, 155, 821, 871
AsuC2I CCSGG 1 cut(s) 603
AsuHPI GGTGA 2 cut(s) 44, 757
AvaII GGWCC 2 cut(s) 821, 871
BaeGI GKGCMC 1 cut(s) 158
BamHI GGATCC 1 cut(s) 750
BanII GRGCYC 1 cut(s) 158
BbsI GAAGAC 1 cut(s) 711
BbvI GCAGC 1 cut(s) 73
BccI CCATC 1 cut(s) 812
BciT130I CCWGG 2 cut(s) 194, 860
BcnI CCSGG 1 cut(s) 603
BcoDI GTCTC 2 cut(s) 464, 848
BfmI CTRYAG 1 cut(s) 894
BfuAI ACCTGC 1 cut(s) 733
BisI GCNGC 2 cut(s) 87, 897
BlpI GCTNAGC 1 cut(s) 102
BlsI GCNGC 2 cut(s) 88, 898
Bme1390I CCNGG 3 cut(s) 194, 603, 860
Bme18I GGWCC 2 cut(s) 821, 871
BmgT120I GGNCC 4 cut(s) 154, 155, 821, 871
BmiI GGNNCC 2 cut(s) 156, 752
BmrFI CCNGG 3 cut(s) 194, 603, 860
BmsI GCATC 3 cut(s) 18, 671, 817
BpiI GAAGAC 1 cut(s) 711
Bpu1102I GCTNAGC 1 cut(s) 102
BpuEI CTTGAG 1 cut(s) 857
BpuMI CCSGG 1 cut(s) 603
BsaI GGTCTC 1 cut(s) 848
BsaJI CCNNGG 2 cut(s) 569, 858
BsaXI ACNNNNNCTCC 2 cut(s) 716, 746
Bsc4I CCNNNNNNNGG 1 cut(s) 487
Bse118I RCCGGY 1 cut(s) 887
Bse1I ACTGG 2 cut(s) 352, 800
BseBI CCWGG 2 cut(s) 194, 860
BseDI CCNNGG 2 cut(s) 569, 858
BseGI GGATG 1 cut(s) 9
BseLI CCNNNNNNNGG 1 cut(s) 487
BseMII CTCAG 4 cut(s) 93, 388, 459, 843
BseNI ACTGG 2 cut(s) 352, 800
BseSI GKGCMC 1 cut(s) 158
BseXI GCAGC 1 cut(s) 73
BseYI CCCAGC 2 cut(s) 157, 425
BshFI GGCC 2 cut(s) 156, 799
BsiSI CCGG 2 cut(s) 603, 888
BslFI GGGAC 1 cut(s) 191
BslI CCNNNNNNNGG 1 cut(s) 487
BsmAI GTCTC 2 cut(s) 464, 848
BsmFI GGGAC 1 cut(s) 191
BsmI GAATGC 1 cut(s) 307
BsnI GGCC 2 cut(s) 156, 799
Bso31I GGTCTC 1 cut(s) 848
Bsp120I GGGCCC 1 cut(s) 154
Bsp1286I GDGCHC 1 cut(s) 158
Bsp143I GATC 5 cut(s) 67, 142, 688, 750, 862
Bsp1720I GCTNAGC 1 cut(s) 102
BspANI GGCC 2 cut(s) 156, 799
BspCNI CTCAG 4 cut(s) 94, 387, 460, 844
BspLI GGNNCC 2 cut(s) 156, 752
BspMAI CTGCAG 1 cut(s) 898
BspMI ACCTGC 1 cut(s) 733
BspPI GGATC 5 cut(s) 75, 683, 745, 758, 870
BspTNI GGTCTC 1 cut(s) 848
BsrFI RCCGGY 1 cut(s) 887
BsrI ACTGG 2 cut(s) 352, 800
BssAI RCCGGY 1 cut(s) 887
BssECI CCNNGG 2 cut(s) 569, 858
BssMI GATC 5 cut(s) 67, 142, 688, 750, 862
BssT1I CCWWGG 1 cut(s) 569
Bst2UI CCWGG 2 cut(s) 194, 860
Bst4CI ACNGT 2 cut(s) 51, 323
BstC8I GCNNGC 5 cut(s) 328, 462, 608, 885, 889
BstDEI CTNAG 4 cut(s) 102, 374, 468, 852
BstF5I GGATG 1 cut(s) 9
BstHHI GCGC 2 cut(s) 129, 742
BstKTI GATC 5 cut(s) 70, 145, 691, 753, 865
BstMAI GTCTC 2 cut(s) 464, 848
BstMBI GATC 5 cut(s) 67, 142, 688, 750, 862
BstMWI GCNNNNNNNGC 2 cut(s) 805, 893
BstNI CCWGG 2 cut(s) 194, 860
BstNSI RCATGY 1 cut(s) 232
BstSCI CCNGG 3 cut(s) 192, 601, 858
BstSFI CTRYAG 1 cut(s) 894
BstSLI GKGCMC 1 cut(s) 158
BstV1I GCAGC 1 cut(s) 73
BstV2I GAAGAC 1 cut(s) 711
BstX2I RGATCY 2 cut(s) 750, 862
BstXI CCANNNNNNTGG 2 cut(s) 647, 699
BstYI RGATCY 2 cut(s) 750, 862
BsuRI GGCC 2 cut(s) 156, 799
BtsCI GGATG 1 cut(s) 9
BtsIMutI CAGTG 1 cut(s) 328
BveI ACCTGC 1 cut(s) 733
Cac8I GCNNGC 5 cut(s) 328, 462, 608, 885, 889
CaiI CAGNNNCTG 1 cut(s) 851
CfoI GCGC 2 cut(s) 129, 742
Cfr10I RCCGGY 1 cut(s) 887
Cfr13I GGNCC 4 cut(s) 154, 155, 821, 871
Csp6I GTAC 1 cut(s) 574
CspCI CAANNNNNGTGG 3 cut(s) 37, 315, 350
CviAII CATG 5 cut(s) 130, 229, 298, 647, 699
CviQI GTAC 1 cut(s) 574
DdeI CTNAG 4 cut(s) 102, 374, 468, 852
DpnI GATC 5 cut(s) 69, 144, 690, 752, 864
DpnII GATC 5 cut(s) 67, 142, 688, 750, 862
Eco130I CCWWGG 1 cut(s) 569
Eco24I GRGCYC 1 cut(s) 158
Eco31I GGTCTC 1 cut(s) 848
Eco47I GGWCC 2 cut(s) 821, 871
Eco57I CTGAAG 1 cut(s) 778
EcoO109I RGGNCCY 1 cut(s) 154
EcoRII CCWGG 2 cut(s) 192, 858
EcoT14I CCWWGG 1 cut(s) 569
EcoT38I GRGCYC 1 cut(s) 158
ErhI CCWWGG 1 cut(s) 569
FaeI CATG 5 cut(s) 133, 232, 301, 650, 702
FaqI GGGAC 1 cut(s) 191
FatI CATG 5 cut(s) 129, 228, 297, 646, 698
Fnu4HI GCNGC 2 cut(s) 87, 897
FriOI GRGCYC 1 cut(s) 158
Fsp4HI GCNGC 2 cut(s) 87, 897
FspI TGCGCA 1 cut(s) 741
GlaI GCGC 2 cut(s) 128, 741
GluI GCNGC 2 cut(s) 87, 897
GsaI CCCAGC 2 cut(s) 161, 429
HaeIII GGCC 2 cut(s) 156, 799
HapII CCGG 2 cut(s) 603, 888
HhaI GCGC 2 cut(s) 129, 742
Hin1II CATG 5 cut(s) 133, 232, 301, 650, 702
Hin6I GCGC 2 cut(s) 127, 740
HinP1I GCGC 2 cut(s) 127, 740
HindIII AAGCTT 2 cut(s) 328, 667
HinfI GANTC 4 cut(s) 242, 581, 725, 788
HpaII CCGG 2 cut(s) 603, 888
HphI GGTGA 2 cut(s) 44, 757
Hpy166II GTNNAC 3 cut(s) 47, 310, 563
Hpy188III TCNNGA 5 cut(s) 211, 451, 779, 836, 851
Hpy8I GTNNAC 3 cut(s) 47, 310, 563
HpyAV CCTTC 1 cut(s) 802
HpyCH4III ACNGT 2 cut(s) 51, 323
HpyF10VI GCNNNNNNNGC 2 cut(s) 805, 893
HpyF3I CTNAG 4 cut(s) 102, 374, 468, 852
Hsp92II CATG 5 cut(s) 133, 232, 301, 650, 702
HspAI GCGC 2 cut(s) 127, 740
KroI GCCGGC 1 cut(s) 887
KroNI GCCGGC 1 cut(s) 889
Kzo9I GATC 5 cut(s) 67, 142, 688, 750, 862
Lsp1109I GCAGC 1 cut(s) 73
LweI GCATC 3 cut(s) 18, 671, 817
MaeIII GTNAC 2 cut(s) 350, 401
MalI GATC 5 cut(s) 69, 144, 690, 752, 864
MboI GATC 5 cut(s) 67, 142, 688, 750, 862
MboII GAAGA 5 cut(s) 209, 548, 716, 794, 797
MfeI CAATTG 1 cut(s) 26
MflI RGATCY 2 cut(s) 750, 862
MhlI GDGCHC 1 cut(s) 158
MluCI AATT 6 cut(s) 26, 95, 182, 206, 259, 625
MlyI GAGTC 2 cut(s) 236, 734
MmeI TCCRAC 1 cut(s) 716
MnlI CCTC 9 cut(s) 58, 105, 415, 481, 491, 576, 621, 652, 741
MroNI GCCGGC 1 cut(s) 887
MroXI GAANNNNTTC 1 cut(s) 446
MseI TTAA 3 cut(s) 80, 219, 225
MslI CAYNNNNRTG 2 cut(s) 813, 816
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 2 cut(s) 603, 888
MspR9I CCNGG 3 cut(s) 194, 603, 860
MunI CAATTG 1 cut(s) 26
Mva1269I GAATGC 1 cut(s) 307
MvaI CCWGG 2 cut(s) 194, 860
MwoI GCNNNNNNNGC 2 cut(s) 805, 893
NaeI GCCGGC 1 cut(s) 889
NciI CCSGG 1 cut(s) 603
NdeII GATC 5 cut(s) 67, 142, 688, 750, 862
NgoMIV GCCGGC 1 cut(s) 887
NlaIII CATG 5 cut(s) 133, 232, 301, 650, 702
NlaIV GGNNCC 2 cut(s) 156, 752
NsbI TGCGCA 1 cut(s) 741
NspI RCATGY 1 cut(s) 232
PaqCI CACCTGC 1 cut(s) 733
PciI ACATGT 1 cut(s) 228
PctI GAATGC 1 cut(s) 307
PdiI GCCGGC 1 cut(s) 889
PdmI GAANNNNTTC 1 cut(s) 446
PfeI GAWTC 2 cut(s) 581, 788
PfoI TCCNGGA 1 cut(s) 192
PkrI GCNGC 2 cut(s) 88, 898
PleI GAGTC 2 cut(s) 236, 733
PpsI GAGTC 2 cut(s) 236, 733
PscI ACATGT 1 cut(s) 228
Psp6I CCWGG 2 cut(s) 192, 858
PspFI CCCAGC 2 cut(s) 157, 425
PspGI CCWGG 2 cut(s) 192, 858
PspN4I GGNNCC 2 cut(s) 156, 752
PspOMI GGGCCC 1 cut(s) 154
PspPI GGNCC 4 cut(s) 154, 155, 821, 871
PstI CTGCAG 1 cut(s) 898
PstNI CAGNNNCTG 1 cut(s) 851
PsuI RGATCY 2 cut(s) 750, 862
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 1 cut(s) 575
RsaNI GTAC 1 cut(s) 574
RseI CAYNNNNRTG 2 cut(s) 813, 816
SaqAI TTAA 3 cut(s) 80, 219, 225
SatI GCNGC 2 cut(s) 87, 897
Sau3AI GATC 5 cut(s) 67, 142, 688, 750, 862
Sau96I GGNCC 4 cut(s) 154, 155, 821, 871
SchI GAGTC 2 cut(s) 236, 734
ScrFI CCNGG 3 cut(s) 194, 603, 860
SduI GDGCHC 1 cut(s) 158
SfaNI GCATC 3 cut(s) 18, 671, 817
SfcI CTRYAG 1 cut(s) 894
SinI GGWCC 2 cut(s) 821, 871
SmiMI CAYNNNNRTG 2 cut(s) 813, 816
SmlI CTYRAG 1 cut(s) 836
SmoI CTYRAG 1 cut(s) 836
Sse9I AATT 6 cut(s) 26, 95, 182, 206, 259, 625
StyD4I CCNGG 3 cut(s) 192, 601, 858
StyI CCWWGG 1 cut(s) 569
TaaI ACNGT 2 cut(s) 51, 323
TaqI TCGA 2 cut(s) 53, 210
TasI AATT 6 cut(s) 26, 95, 182, 206, 259, 625
TfiI GAWTC 2 cut(s) 581, 788
Tru1I TTAA 3 cut(s) 80, 219, 225
Tru9I TTAA 3 cut(s) 80, 219, 225
TscAI CASTG 1 cut(s) 328
TseI GCWGC 2 cut(s) 86, 896
TspDTI ATGAA 3 cut(s) 146, 270, 314
TspGWI ACGGA 2 cut(s) 743, 821
TspRI CASTG 1 cut(s) 328
VpaK11BI GGWCC 2 cut(s) 821, 871
XapI RAATTY 3 cut(s) 182, 206, 259
XceI RCATGY 1 cut(s) 232
XmnI GAANNNNTTC 1 cut(s) 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.