RLG00000015572

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002574
Physical Location & Seq
Forward (+)
67972850 .. 67974333
1484 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015572

Sequence Viewer

Length: 915 bp
ATGAGTAGTAGCTCTGTGTGGCAGTTGTCAGTTACTACTCGTAAGAATGATGTACAATATCATCATAAACCGGTGGTCACGGCCTCCCAAGAAGCTGATCATGATCAGTATCAAACGCGGACAGCAGCAACAAGAGTAGCACTGATTGACAATCAATTGATGCAAATGACTACTACTGCCGCAGAATACTCAGATGATGAAGATCATAATAAGAAGGATTATTATTATCGTTATGGAAAGAGTAGTAGTTCCAATTTGAAGAACAACAACAATATCAGTTATGCGGCATTGAAATTGTTAGAGATGAAATTACGCGGCGTGCAGGCTGTGCAGCATAGCAATAAGTCCCTTCATTACTATGTGCAGCAACAGCAGCAGCAGAGTGACAGTGAGAGTAGTGAGATGATGAGCAGTAGTCATGACAATTATTATTGTTATTACAATAAGAAGCAGCAGGTGTGGCATAAGACGCTGCCTCAGGCTGAAATGCTGCCTAGAAATGAAGTCTTGGGAGGTTACATTTTTGTATGCAACAACGAAACCATGCAAGAGGATCTCCACCGTCAACTCTTCGGACTCCCTCAAAAGTACAAGGACTCTGTAAGGGCAATTACCCCAGGCTTACCCCTTTTTCTTTACAACTACACAGCCCACCAGCTCCATGGAGTTTTTCAGGCTGTGAGTTTTGGAGGATCAAACATCGATGCAACAGCGTGGGAAGACAAGAAATCCAAAGGAGAATCAAGATTTCCAGCACAAGTTAGAATTCGAGCCAAGGAAAGGTGCAAACCACTAGAAGAGGATGCTTTTAGGCCAATACTCTACCACTATGATGGCCCAAAGTTTCGTTTACAACTCTCTGTGCCTGAGGTGCTTGCATTGTTAGACTTATTCAAAGAAGATTACCGGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

305

Amino Acids

35.29

Weight (kDa)

7.73

Isoelectric Point (pI)

53.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 171 - 298 7.5e-41 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 445
Acc36I ACCTGC 1 cut(s) 445
AccII CGCG 2 cut(s) 118, 315
AciI CCGC 4 cut(s) 118, 180, 284, 315
AclWI GGATC 2 cut(s) 561, 700
AcsI RAATTY 1 cut(s) 765
AfaI GTAC 2 cut(s) 54, 590
AfiI CCNNNNNNNGG 1 cut(s) 780
AgeI ACCGGT 1 cut(s) 70
AgsI TTSAA 3 cut(s) 259, 292, 895
AjnI CCWGG 1 cut(s) 616
AluBI AGCT 3 cut(s) 12, 95, 658
AluI AGCT 3 cut(s) 12, 95, 658
AlwI GGATC 2 cut(s) 561, 700
AoxI GGCC 3 cut(s) 81, 812, 835
ApeKI GCWGC 8 cut(s) 125, 331, 364, 373, 376, 451, 472, 490
ApoI RAATTY 1 cut(s) 765
AsiGI ACCGGT 1 cut(s) 70
AspS9I GGNCC 1 cut(s) 836
AxyI CCTNAGG 2 cut(s) 477, 867
BbsI GAAGAC 1 cut(s) 726
BbvI GCAGC 8 cut(s) 137, 343, 376, 385, 388, 459, 463, 477
BccI CCATC 1 cut(s) 827
BceAI ACGGC 1 cut(s) 96
BciT130I CCWGG 1 cut(s) 618
BclI TGATCA 2 cut(s) 97, 103
BfaI CTAG 2 cut(s) 495, 794
BfuAI ACCTGC 1 cut(s) 445
Bme1390I CCNGG 1 cut(s) 618
BmgT120I GGNCC 1 cut(s) 836
BmrFI CCNGG 1 cut(s) 618
BmsI GCATC 3 cut(s) 150, 694, 793
BpiI GAAGAC 1 cut(s) 726
Bsa29I ATCGAT 1 cut(s) 702
BsaBI GATNNNNATC 3 cut(s) 102, 108, 201
BsaJI CCNNGG 3 cut(s) 616, 661, 774
BsaWI WCCGGW 1 cut(s) 70
Bsc4I CCNNNNNNNGG 1 cut(s) 780
Bse118I RCCGGY 2 cut(s) 70, 906
Bse21I CCTNAGG 2 cut(s) 477, 867
Bse8I GATNNNNATC 3 cut(s) 102, 108, 201
BseBI CCWGG 1 cut(s) 618
BseCI ATCGAT 1 cut(s) 702
BseDI CCNNGG 3 cut(s) 616, 661, 774
BseGI GGATG 1 cut(s) 808
BseJI GATNNNNATC 3 cut(s) 102, 108, 201
BseLI CCNNNNNNNGG 1 cut(s) 780
BseMII CTCAG 3 cut(s) 204, 491, 858
BseXI GCAGC 8 cut(s) 137, 343, 376, 385, 388, 459, 463, 477
BsgI GTGCAG 3 cut(s) 341, 350, 383
Bsh1236I CGCG 2 cut(s) 118, 315
BshFI GGCC 3 cut(s) 83, 814, 837
BshTI ACCGGT 1 cut(s) 70
BshVI ATCGAT 1 cut(s) 702
BsiSI CCGG 2 cut(s) 71, 907
BslFI GGGAC 1 cut(s) 331
BslI CCNNNNNNNGG 1 cut(s) 780
BsmFI GGGAC 1 cut(s) 331
BsnI GGCC 3 cut(s) 83, 814, 837
Bsp1407I TGTACA 1 cut(s) 52
Bsp143I GATC 5 cut(s) 97, 103, 202, 553, 692
Bsp19I CCATGG 1 cut(s) 661
BspACI CCGC 4 cut(s) 118, 180, 284, 315
BspANI GGCC 3 cut(s) 83, 814, 837
BspCNI CTCAG 3 cut(s) 203, 490, 859
BspDI ATCGAT 1 cut(s) 702
BspFNI CGCG 2 cut(s) 118, 315
BspHI TCATGA 2 cut(s) 100, 418
BspMI ACCTGC 1 cut(s) 445
BspPI GGATC 2 cut(s) 561, 700
BsrFI RCCGGY 2 cut(s) 70, 906
BsrGI TGTACA 1 cut(s) 52
BssAI RCCGGY 2 cut(s) 70, 906
BssECI CCNNGG 3 cut(s) 616, 661, 774
BssMI GATC 5 cut(s) 97, 103, 202, 553, 692
BssT1I CCWWGG 2 cut(s) 661, 774
Bst2UI CCWGG 1 cut(s) 618
Bst4CI ACNGT 2 cut(s) 389, 563
Bst6I CTCTTC 2 cut(s) 575, 792
BstAPI GCANNNNNTGC 1 cut(s) 328
BstAUI TGTACA 1 cut(s) 52
BstC8I GCNNGC 3 cut(s) 320, 324, 876
BstDEI CTNAG 3 cut(s) 190, 477, 867
BstDSI CCRYGG 1 cut(s) 661
BstF5I GGATG 1 cut(s) 808
BstFNI CGCG 2 cut(s) 118, 315
BstKTI GATC 5 cut(s) 100, 106, 205, 556, 695
BstMBI GATC 5 cut(s) 97, 103, 202, 553, 692
BstMWI GCNNNNNNNGC 6 cut(s) 328, 370, 373, 460, 469, 871
BstNI CCWGG 1 cut(s) 618
BstSCI CCNGG 1 cut(s) 616
BstUI CGCG 2 cut(s) 118, 315
BstV1I GCAGC 8 cut(s) 137, 343, 376, 385, 388, 459, 463, 477
BstV2I GAAGAC 1 cut(s) 726
BstX2I RGATCY 1 cut(s) 553
BstXI CCANNNNNNTGG 2 cut(s) 662, 833
BstYI RGATCY 1 cut(s) 553
Bsu15I ATCGAT 1 cut(s) 702
Bsu36I CCTNAGG 2 cut(s) 477, 867
BsuRI GGCC 3 cut(s) 83, 814, 837
BsuTUI ATCGAT 1 cut(s) 702
BtgI CCRYGG 1 cut(s) 661
BtsCI GGATG 1 cut(s) 808
BtsIMutI CAGTG 2 cut(s) 140, 394
BveI ACCTGC 1 cut(s) 445
Cac8I GCNNGC 3 cut(s) 320, 324, 876
CciI TCATGA 2 cut(s) 100, 418
Cfr10I RCCGGY 2 cut(s) 70, 906
Cfr13I GGNCC 1 cut(s) 836
ClaI ATCGAT 1 cut(s) 702
CseI GACGC 1 cut(s) 478
Csp6I GTAC 2 cut(s) 53, 589
CspAI ACCGGT 1 cut(s) 70
CviAII CATG 4 cut(s) 101, 419, 544, 662
CviQI GTAC 2 cut(s) 53, 589
DdeI CTNAG 3 cut(s) 190, 477, 867
DpnI GATC 5 cut(s) 99, 105, 204, 555, 694
DpnII GATC 5 cut(s) 97, 103, 202, 553, 692
Eam1104I CTCTTC 2 cut(s) 575, 792
EarI CTCTTC 2 cut(s) 575, 792
Eco130I CCWWGG 2 cut(s) 661, 774
Eco81I CCTNAGG 2 cut(s) 477, 867
EcoRI GAATTC 1 cut(s) 765
EcoRII CCWGG 1 cut(s) 616
EcoT14I CCWWGG 2 cut(s) 661, 774
ErhI CCWWGG 2 cut(s) 661, 774
FaeI CATG 4 cut(s) 104, 422, 547, 665
FaqI GGGAC 1 cut(s) 331
FatI CATG 4 cut(s) 100, 418, 543, 661
FbaI TGATCA 2 cut(s) 97, 103
FokI GGATG 1 cut(s) 815
FspBI CTAG 2 cut(s) 495, 794
HaeIII GGCC 3 cut(s) 83, 814, 837
HapII CCGG 2 cut(s) 71, 907
HgaI GACGC 1 cut(s) 478
Hin1II CATG 4 cut(s) 104, 422, 547, 665
HincII GTYRAC 1 cut(s) 566
HindII GTYRAC 1 cut(s) 566
HinfI GANTC 3 cut(s) 576, 596, 740
HpaII CCGG 2 cut(s) 71, 907
Hpy166II GTNNAC 2 cut(s) 566, 851
Hpy188I TCNGA 2 cut(s) 193, 575
Hpy188III TCNNGA 3 cut(s) 101, 419, 744
Hpy8I GTNNAC 2 cut(s) 566, 851
HpyAV CCTTC 2 cut(s) 208, 359
HpyCH4III ACNGT 2 cut(s) 389, 563
HpyCH4V TGCA 9 cut(s) 163, 322, 331, 364, 531, 547, 707, 786, 878
HpyF10VI GCNNNNNNNGC 6 cut(s) 328, 370, 373, 460, 469, 871
HpyF3I CTNAG 3 cut(s) 190, 477, 867
Hsp92II CATG 4 cut(s) 104, 422, 547, 665
Ksp22I TGATCA 2 cut(s) 97, 103
Kzo9I GATC 5 cut(s) 97, 103, 202, 553, 692
LmnI GCTCC 1 cut(s) 663
Lsp1109I GCAGC 8 cut(s) 137, 343, 376, 385, 388, 459, 463, 477
LweI GCATC 3 cut(s) 150, 694, 793
MaeI CTAG 2 cut(s) 495, 794
MaeIII GTNAC 4 cut(s) 31, 76, 383, 515
MalI GATC 5 cut(s) 99, 105, 204, 555, 694
MboI GATC 5 cut(s) 97, 103, 202, 553, 692
MboII GAAGA 6 cut(s) 212, 271, 562, 731, 809, 911
MfeI CAATTG 1 cut(s) 155
MflI RGATCY 1 cut(s) 553
MluCI AATT 7 cut(s) 155, 253, 293, 308, 424, 609, 765
MlyI GAGTC 2 cut(s) 570, 590
MnlI CCTC 8 cut(s) 94, 486, 506, 544, 591, 683, 793, 862
MslI CAYNNNNRTG 2 cut(s) 357, 831
MspI CCGG 2 cut(s) 71, 907
MspR9I CCNGG 1 cut(s) 618
MunI CAATTG 1 cut(s) 155
MvaI CCWGG 1 cut(s) 618
MvnI CGCG 2 cut(s) 118, 315
MwoI GCNNNNNNNGC 6 cut(s) 328, 370, 373, 460, 469, 871
NcoI CCATGG 1 cut(s) 661
NdeII GATC 5 cut(s) 97, 103, 202, 553, 692
NlaIII CATG 4 cut(s) 104, 422, 547, 665
NmuCI GTSAC 2 cut(s) 76, 383
PagI TCATGA 2 cut(s) 100, 418
PaqCI CACCTGC 1 cut(s) 445
PfeI GAWTC 1 cut(s) 740
PinAI ACCGGT 1 cut(s) 70
PleI GAGTC 2 cut(s) 570, 590
PpsI GAGTC 2 cut(s) 570, 590
Psp6I CCWGG 1 cut(s) 616
PspGI CCWGG 1 cut(s) 616
PspPI GGNCC 1 cut(s) 836
PsuI RGATCY 1 cut(s) 553
RsaI GTAC 2 cut(s) 54, 590
RsaNI GTAC 2 cut(s) 53, 589
RseI CAYNNNNRTG 2 cut(s) 357, 831
Sau3AI GATC 5 cut(s) 97, 103, 202, 553, 692
Sau96I GGNCC 1 cut(s) 836
SchI GAGTC 2 cut(s) 570, 590
ScrFI CCNGG 1 cut(s) 618
SetI ASST 7 cut(s) 14, 97, 459, 517, 660, 785, 873
SfaNI GCATC 3 cut(s) 150, 694, 793
SmiMI CAYNNNNRTG 2 cut(s) 357, 831
Sse9I AATT 7 cut(s) 155, 253, 293, 308, 424, 609, 765
SsiI CCGC 4 cut(s) 118, 180, 284, 315
SspMI CTAG 2 cut(s) 495, 794
StyD4I CCNGG 1 cut(s) 616
StyI CCWWGG 2 cut(s) 661, 774
TaaI ACNGT 2 cut(s) 389, 563
TaqI TCGA 2 cut(s) 702, 769
TasI AATT 7 cut(s) 155, 253, 293, 308, 424, 609, 765
TatI WGTACW 2 cut(s) 52, 588
TauI GCSGC 3 cut(s) 182, 287, 318
TfiI GAWTC 1 cut(s) 740
TscAI CASTG 2 cut(s) 147, 394
TseFI GTSAC 2 cut(s) 76, 383
TseI GCWGC 8 cut(s) 125, 331, 364, 373, 376, 451, 472, 490
Tsp45I GTSAC 2 cut(s) 76, 383
TspDTI ATGAA 4 cut(s) 213, 320, 341, 516
TspRI CASTG 2 cut(s) 147, 394
XapI RAATTY 1 cut(s) 765
XcmI CCANNNNNNNNNTGG 1 cut(s) 659
XspI CTAG 2 cut(s) 495, 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.