RchiOBHm_Chr6g0249531

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0002574
Physical Location & Seq
Reverse (-)
5506680 .. 5507620
941 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22371

Sequence Viewer

Length: 483 bp
ATGAGCAGTAGTCATGACAATGATTATTGTTATTACAATAAGCAGCAGCAGGTGTGGCATAAGACGCTGCCTCAGGCTGAAATGCTGCCTAGAAATGAAGTCTTGGGAGGTTACATTTTTGTATGCAACAACGAAACCATGCAAGAGGATCTCCACCGTCAACTCTTCGGACTCCCTCAAAAGTACAAGGACTCCGTAAGGGCAATTACCCCAGGCTTACCCCTTTTTCTTTACAACTACACAGCCCACCAGCTCCATGGAGTTTTTCAGGCTGTGAGTTTTGGAGGATCAAACATCGATCCAACAGCGTGGGAAGACAAGAAATCCAAAGGAGAATCAAGATTTCCAGCACAAGTTAGAATTCGAGCCAAGGAAAGGTGCAAACCACTAGAAGAGGATGCTTTTAGGCCAATACTCTACCACTATGATGGCCCGAAGTTTCGTTTACAACTCTCTGTGCCTGAGGTACCTACTAGCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

160

Amino Acids

18.6

Weight (kDa)

7.74

Isoelectric Point (pI)

57.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 36 - 156 5.6e-40 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 40
Acc36I ACCTGC 1 cut(s) 40
Acc65I GGTACC 1 cut(s) 466
AccB1I GGYRCC 1 cut(s) 466
AclWI GGATC 3 cut(s) 156, 293, 295
AcsI RAATTY 1 cut(s) 360
AfaI GTAC 2 cut(s) 185, 468
AfiI CCNNNNNNNGG 1 cut(s) 375
AjnI CCWGG 1 cut(s) 211
AluBI AGCT 2 cut(s) 253, 477
AluI AGCT 2 cut(s) 253, 477
AlwI GGATC 3 cut(s) 156, 293, 295
AoxI GGCC 2 cut(s) 407, 430
ApeKI GCWGC 4 cut(s) 43, 46, 67, 85
ApoI RAATTY 1 cut(s) 360
Asp718I GGTACC 1 cut(s) 466
AspS9I GGNCC 1 cut(s) 431
AxyI CCTNAGG 2 cut(s) 72, 462
BanI GGYRCC 1 cut(s) 466
BbsI GAAGAC 1 cut(s) 321
BbvI GCAGC 4 cut(s) 54, 55, 58, 72
BccI CCATC 1 cut(s) 422
BciT130I CCWGG 1 cut(s) 213
BfaI CTAG 3 cut(s) 90, 389, 474
BfuAI ACCTGC 1 cut(s) 40
BisI GCNGC 4 cut(s) 44, 47, 68, 86
BlsI GCNGC 4 cut(s) 45, 48, 69, 87
Bme1390I CCNGG 1 cut(s) 213
BmgT120I GGNCC 1 cut(s) 431
BmiI GGNNCC 1 cut(s) 468
BmrFI CCNGG 1 cut(s) 213
BmsI GCATC 1 cut(s) 388
BpiI GAAGAC 1 cut(s) 321
Bsa29I ATCGAT 1 cut(s) 297
BsaJI CCNNGG 3 cut(s) 211, 256, 369
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bsc4I CCNNNNNNNGG 1 cut(s) 375
Bse21I CCTNAGG 2 cut(s) 72, 462
BseBI CCWGG 1 cut(s) 213
BseCI ATCGAT 1 cut(s) 297
BseDI CCNNGG 3 cut(s) 211, 256, 369
BseGI GGATG 1 cut(s) 403
BseLI CCNNNNNNNGG 1 cut(s) 375
BseMII CTCAG 2 cut(s) 86, 453
BseXI GCAGC 4 cut(s) 54, 55, 58, 72
BshFI GGCC 2 cut(s) 409, 432
BshNI GGYRCC 1 cut(s) 466
BshVI ATCGAT 1 cut(s) 297
BslI CCNNNNNNNGG 1 cut(s) 375
BsnI GGCC 2 cut(s) 409, 432
Bsp143I GATC 3 cut(s) 148, 287, 298
Bsp19I CCATGG 1 cut(s) 256
BspANI GGCC 2 cut(s) 409, 432
BspCNI CTCAG 2 cut(s) 85, 454
BspDI ATCGAT 1 cut(s) 297
BspHI TCATGA 1 cut(s) 13
BspLI GGNNCC 1 cut(s) 468
BspMI ACCTGC 1 cut(s) 40
BspPI GGATC 3 cut(s) 156, 293, 295
BspT107I GGYRCC 1 cut(s) 466
BssECI CCNNGG 3 cut(s) 211, 256, 369
BssMI GATC 3 cut(s) 148, 287, 298
BssT1I CCWWGG 2 cut(s) 256, 369
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 1 cut(s) 158
Bst6I CTCTTC 2 cut(s) 170, 387
BstDEI CTNAG 2 cut(s) 72, 462
BstDSI CCRYGG 1 cut(s) 256
BstF5I GGATG 1 cut(s) 403
BstKTI GATC 3 cut(s) 151, 290, 301
BstMBI GATC 3 cut(s) 148, 287, 298
BstMWI GCNNNNNNNGC 2 cut(s) 55, 64
BstNI CCWGG 1 cut(s) 213
BstSCI CCNGG 1 cut(s) 211
BstV1I GCAGC 4 cut(s) 54, 55, 58, 72
BstV2I GAAGAC 1 cut(s) 321
BstX2I RGATCY 1 cut(s) 148
BstXI CCANNNNNNTGG 3 cut(s) 257, 309, 428
BstYI RGATCY 1 cut(s) 148
Bsu15I ATCGAT 1 cut(s) 297
Bsu36I CCTNAGG 2 cut(s) 72, 462
BsuRI GGCC 2 cut(s) 409, 432
BsuTUI ATCGAT 1 cut(s) 297
BtgI CCRYGG 1 cut(s) 256
BtsCI GGATG 1 cut(s) 403
BveI ACCTGC 1 cut(s) 40
CciI TCATGA 1 cut(s) 13
Cfr13I GGNCC 1 cut(s) 431
ClaI ATCGAT 1 cut(s) 297
CseI GACGC 1 cut(s) 73
Csp6I GTAC 2 cut(s) 184, 467
CviAII CATG 3 cut(s) 14, 139, 257
CviJI RGCY 9 cut(s) 77, 216, 245, 253, 272, 368, 409, 432, 477
CviKI_1 RGCY 9 cut(s) 77, 216, 245, 253, 272, 368, 409, 432, 477
CviQI GTAC 2 cut(s) 184, 467
DdeI CTNAG 2 cut(s) 72, 462
DpnI GATC 3 cut(s) 150, 289, 300
DpnII GATC 3 cut(s) 148, 287, 298
Eam1104I CTCTTC 2 cut(s) 170, 387
EarI CTCTTC 2 cut(s) 170, 387
Eco130I CCWWGG 2 cut(s) 256, 369
Eco81I CCTNAGG 2 cut(s) 72, 462
EcoRI GAATTC 1 cut(s) 360
EcoRII CCWGG 1 cut(s) 211
EcoT14I CCWWGG 2 cut(s) 256, 369
ErhI CCWWGG 2 cut(s) 256, 369
FaeI CATG 3 cut(s) 17, 142, 260
FaiI YATR 6 cut(s) 15, 60, 124, 140, 258, 426
FatI CATG 3 cut(s) 13, 138, 256
Fnu4HI GCNGC 4 cut(s) 44, 47, 68, 86
FokI GGATG 1 cut(s) 410
Fsp4HI GCNGC 4 cut(s) 44, 47, 68, 86
FspBI CTAG 3 cut(s) 90, 389, 474
GluI GCNGC 4 cut(s) 44, 47, 68, 86
HaeIII GGCC 2 cut(s) 409, 432
HgaI GACGC 1 cut(s) 73
Hin1II CATG 3 cut(s) 17, 142, 260
HincII GTYRAC 1 cut(s) 161
HindII GTYRAC 1 cut(s) 161
HinfI GANTC 3 cut(s) 171, 191, 335
Hpy166II GTNNAC 2 cut(s) 161, 446
Hpy188I TCNGA 1 cut(s) 170
Hpy188III TCNNGA 2 cut(s) 14, 339
Hpy8I GTNNAC 2 cut(s) 161, 446
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4V TGCA 3 cut(s) 126, 142, 381
HpyF10VI GCNNNNNNNGC 2 cut(s) 55, 64
HpyF3I CTNAG 2 cut(s) 72, 462
Hsp92II CATG 3 cut(s) 17, 142, 260
KpnI GGTACC 1 cut(s) 470
Kzo9I GATC 3 cut(s) 148, 287, 298
LmnI GCTCC 1 cut(s) 258
LpnPI CCDG 8 cut(s) 35, 59, 198, 225, 254, 263, 360, 474
Lsp1109I GCAGC 4 cut(s) 54, 55, 58, 72
LweI GCATC 1 cut(s) 388
MaeI CTAG 3 cut(s) 90, 389, 474
MaeIII GTNAC 1 cut(s) 110
MalI GATC 3 cut(s) 150, 289, 300
MboI GATC 3 cut(s) 148, 287, 298
MboII GAAGA 3 cut(s) 157, 326, 404
MflI RGATCY 1 cut(s) 148
MluCI AATT 2 cut(s) 204, 360
MlyI GAGTC 2 cut(s) 165, 185
MmeI TCCRAC 1 cut(s) 326
MnlI CCTC 7 cut(s) 81, 101, 139, 186, 278, 388, 457
MslI CAYNNNNRTG 2 cut(s) 18, 426
MspR9I CCNGG 1 cut(s) 213
MvaI CCWGG 1 cut(s) 213
MwoI GCNNNNNNNGC 2 cut(s) 55, 64
NcoI CCATGG 1 cut(s) 256
NdeII GATC 3 cut(s) 148, 287, 298
NlaIII CATG 3 cut(s) 17, 142, 260
NlaIV GGNNCC 1 cut(s) 468
PagI TCATGA 1 cut(s) 13
PaqCI CACCTGC 1 cut(s) 40
PfeI GAWTC 1 cut(s) 335
PkrI GCNGC 4 cut(s) 45, 48, 69, 87
PleI GAGTC 2 cut(s) 165, 185
PpsI GAGTC 2 cut(s) 165, 185
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 1 cut(s) 468
PspPI GGNCC 1 cut(s) 431
PsuI RGATCY 1 cut(s) 148
RsaI GTAC 2 cut(s) 185, 468
RsaNI GTAC 2 cut(s) 184, 467
RseI CAYNNNNRTG 2 cut(s) 18, 426
SatI GCNGC 4 cut(s) 44, 47, 68, 86
Sau3AI GATC 3 cut(s) 148, 287, 298
Sau96I GGNCC 1 cut(s) 431
SchI GAGTC 2 cut(s) 165, 185
ScrFI CCNGG 1 cut(s) 213
SetI ASST 7 cut(s) 54, 112, 255, 380, 468, 472, 479
SfaNI GCATC 1 cut(s) 388
SmiMI CAYNNNNRTG 2 cut(s) 18, 426
Sse9I AATT 2 cut(s) 204, 360
SspMI CTAG 3 cut(s) 90, 389, 474
StyD4I CCNGG 1 cut(s) 211
StyI CCWWGG 2 cut(s) 256, 369
TaaI ACNGT 1 cut(s) 158
TaqI TCGA 2 cut(s) 297, 364
TasI AATT 2 cut(s) 204, 360
TatI WGTACW 1 cut(s) 183
TfiI GAWTC 1 cut(s) 335
TseI GCWGC 4 cut(s) 43, 46, 67, 85
TspDTI ATGAA 1 cut(s) 111
TspGWI ACGGA 1 cut(s) 184
XapI RAATTY 1 cut(s) 360
XcmI CCANNNNNNNNNTGG 1 cut(s) 254
XspI CTAG 3 cut(s) 90, 389, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.