Rorug02G0586000

B2 protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002574
Physical Location & Seq
Reverse (-)
70888039 .. 70892614
4576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0586000.1

Sequence Viewer

Length: 3798 bp
ATGAGACGAAGCAGCACCACCATAGCTCTATGCAATGAAATGCTTCAAGTTTTGTGTACCTGTTCACCCCACAAGCCTCACATCAAACAGGTTGGTTCTCTTTACTCTCTGTTCTCACTCTTCACCACTAACCACCACTTCTCAACTACAACTTCTTCATCCCCATCATCTTCAACTACACAAAATACCAACACCCAAATAGATTCATCTCCCAATTGTTTCAATGGTATAGCCCAATCTGTCATTTTGAGGTGTTCCCAGTATTTTGACAAGAGTAAAGGCAAAGACTTTGCCAATGCTTCTCTAAAAGACCTTCTTTTGGAGATTTCTGATTTAGTGCCTGAACAAACTCGTAGGCTTAGGCGGGTTTTGGAGCCGAAACCCGAAGATGTGCTTGAATTATTACTGGGTTTTGAATTGCAGTGTGGGCAAGTTGGATTTGAAGCTAGAAAGGTTGAGTCTTTGTGGGGAATTTTCAAGTGGGTTAGTGAAAACATTAAGGGTTTTAAGCATAAACCTCAGTCATGTGAGGTCATGGCCTCAATGCTTGTCAGAGTGGGATTGCTTAGAAAAGTTGAGTTCTTGCTTTCCACAATGGAAAGTAAAGGAGTTTCGTTGGATAGTCAAGAAATTTACAGTGATTTGATTGAAGGGTATGTTGGTATTGGTGAGTTAGATAGGGCCATTTCGGTATATGATCGAATTAGGGGGCGTGTAGTGCCATCATTGCAGTGTTGTTGTGTTCTACTTGATCAATTGGTGGGAATGAGGAAGACCCAATTGGCGTTTCAAGTTTGTAGTGATATGGCACAAATGGGTTTTGATTTGAGAGATGTGAAGAAGGCCACATTTGAGGGTGTCATTAGACTGCTTTGCAGGGATGGAAAGATTCAGGAGGCAAGAAATTTTGTCAAGAAGGCTATGGCTTTCGAACTCATGCCTAGCAACTTAGTTTTAAATGAAGTTGCTTATGGATATTGTGAGAAGAAGGACTTTGATGATTTGATGAGCTTCTATGCTGAGATTAAGTGTGCCCCAGAAGTTTTGGCTGGGAATAGGATTGTGCATTCTCTTTGTAGCAATTTTGGGACAAGAAGGGCAGAACCATATTTGCAAGAATTAGAAATTTTAGGCTTCAATCCTGATGAGGTAACCTTTGGAATCATGATTGGCTGGAGCTGTCGTGAACGAAAACTTAAAAGTGCCTTTGTTTACCTATCAGAGATGTTGGGAAGACACCTAAACCCCCATATATGTACCTATAATGCTCTTATCAGCGGGGTATTTATGGAGGGTATGTGGAAGCATGCTGGGAAGGTGTTTGATGAGATGGTAGATAGGGGGACTACACCTGATTTGTCGACTTTCAGGATTCTCTTAGCAGGCTATTGTAAAGTTAGAAAATTTGATGAAGCCAAAAGGATAGTTTTTGATATGGCAAGCCATGGACTGATTCAAAACTCTTCAGGTGAGGATCCGCTATCCAAAGCATTTATGGTTCTGGGTTTCAAGCCATTAGCTGTGACATTGAAGAGAGATAATGATGTTGGGTTTGCTAAAACAGAGTTTTATGATGATCTTGGGAATGGACTTTATTTGGATACAGACCTGGATAAGTATGAGAAAAGAGTCACTGGGATCCTAGAAGATTGCATGGTACCTGATTATTACTCTCTTATGATGAAAGAATGCACTCTTGGAAATTTGAAGGGTGCATTGGTTTTGGTAGATGAAATGATCCGGTGGGGACAAGATTTGTCATTGTCTATGATCTCCGACTTACTGAAAGGGCTTTCTGCATCCCATTTACATACCAAGGGAATCACCAGCATTGTAGATAAAAAGCTTCACTTGGTAAGTCAGCTAGACCAAGAAACTCTAAACTTTCTTGCCCAAGCATACGGCAAGAAAGGATTGACATATAATACAAGGATAGTAGTGAATGGAATGATAGAAAGGCATCTGAAAATCAACAACGAGACTTACACTGCTTTAGTAAAAGGTTTTTGTAAGAAGGGAAACTTGAGGGAGCTGAATGCTTGCTGGAATATTGCTCAAAATGAGAGATGGTTACCAGGGTCGGAAGATTGTAAAGCCTTGATGGAATGTCTTTGCAGTAAAGAAATGCTAAGAGAAGCAGTGCAGCTTCTTGAAAGCATTCTGATATCCTATCCAGACTTGAGGTCAGATATGTGTAATATGATCCTAGACAAGCTTATTCTTACAGGTTGTACAGGAATTGCAAGCATATTGTTAGAAGAACTTGAACAGCGTGGTGGCATCCTGGATCGGATGGCCTATAACAATCTTATTAGAGGATTGTGTAAGGACAAGAATTTTCAGGTAGCCTTTACAGTGCTAGACAGTATGCTGGCTAAAAATTTGGCCCCATGTTTGGATGTTATTGTCCAATTAATTCCTCAGTTGTGTAAGGCTGATAGATTTGGAAAAGCAGTTAACTTGAAAGAGATTGGTCTAAGAGAGAAATCTTCATTTCTACTTTCTCTAGATCATGCATTGATAGAAGGCTGTTGTATTTCAGGGAAAGTTACAGAAGCAACCACTCTACTACAGAATATGTTGTTAAATGGGATACCTCCTGATGCTGAAATTTACAACTTTATGGTTCAGGGTCTTTGCAAGGTTAATGACTTGAAGAAAGTATGGGAGCTACTTTGTGTAATGGCAAGAAAAAGTTTCAACATTTCACTCTCAACTTACCGCAATTTGGTGCGTTTGTTGTGTCTGGAAGGTAGGGTTCTTCATGCATGGAAATTGACTGAGCTTATGATTGGACAAAGGGATCCTTATGATCTTAGCTTCTACAATATTCTGCTATTCTATATTTTCCCAACCGGGAATACTTTGCTTGTCAAGAAGGTTGTGGAGCATTTGCAAGAGAAGAAATTGCTACTTGATGAAGTAACTTACAGCTTTCTTGTACTTGGGTTTTGTAGATGTAAAGATGTGTCGAGTGCTGTGGATCATCTGTACACTATGATCTCTAAGGATTTCAGGCCAAGCAACCGCAACTTGAGAAAAGTTATAACCAGCCTGTGTGATATTGGGGAGATTGAGAAAGCCTTGGAATTGAGTCGAGAAATGGAATTGAGAGGCCGGATTCATGATTCAATGATTCAAAATGCGATTGTTGAGGGTCTTTCTTCTCGTGGTAGGATTCAAGAAGCTGAAAATTTTCTGGACAGGATGGTAGAGAAGTGTCTAATTCCAGAAAATGTCAACTATAATAATCTAATTAAACTGTTTTGCTTGTATGGAAGACCGAACAAAGCAGTTAATCTTCTGAACATAATGCTGAAGAAAGGGAATGTTCCAGATTCTACCACTTATGATTCCATCATCAGCTCTTTCAGTGCCCTTGGTAATCTGGAGCAAGCTATGGATTTTCATGCTGAGATGTTGGATAGAGATCTTAAGCCAAGCATCGACACTTGGGACATTCTGGTCCATAACTTTTGTCGAGATGGAAAAACAGCAGAGGCAGAAAGGCTTTTGAAGTATATGGTTTGTGCAGGTGAAACAGTGACCAGGAAGATATATCTATGTGTAATTAATAGTTATCGCTCAGAGAACAATCTCGGAAAGGTCTCAGAGCTTATGCAAGCAATGCAACAGAGTGGTTATGAGCCAGACTTCGAGACACATTGGTCTCTCATAAGGAACCTAAGAGTTTCTAGTGACAAGGACAATGCAAACAGGAGCAAGGGTTTCCTGTCAAAGCTTCTCTCTGCAAGTGGATTTTCTAGGCAAAAGTATTCCAAGGCCAAACAGGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

1265

Amino Acids

143.09

Weight (kDa)

7.82

Isoelectric Point (pI)

34.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 371 - 426 7.8e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 416 - 465 4.5e-14 PPR repeat family
PPR_2 PF13041 867 - 915 2.1e-07 PPR repeat family
TPR_24 PF23276 953 - 1065 4.6e-08 Fungal tetratrico peptide repeats
PPR_2 PF13041 1078 - 1124 4.5e-10 PPR repeat family
PPR_2 PF13041 1112 - 1161 9.4e-09 PPR repeat family
PPR_1 PF12854 1144 - 1176 1.9e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3047
AarI CACCTGC 1 cut(s) 3524
AasI GACNNNNNNGTC 2 cut(s) 3464, 3667
Acc36I ACCTGC 1 cut(s) 3524
Acc65I GGTACC 1 cut(s) 1657
AccB1I GGYRCC 1 cut(s) 1657
AccI GTMKAC 1 cut(s) 1361
AciI CCGC 5 cut(s) 364, 1278, 1478, 2722, 3028
AcuI CTGAAG 2 cut(s) 1449, 3338
AfaI GTAC 6 cut(s) 58, 1258, 1659, 2233, 2943, 2993
AfiI CCNNNNNNNGG 4 cut(s) 319, 2395, 2728, 3792
AflII CTTAAG 1 cut(s) 3434
AhdI GACNNNNNGTC 1 cut(s) 2182
AjnI CCWGG 4 cut(s) 1608, 2074, 2283, 3548
Alw26I GTCTC 4 cut(s) 1973, 3613, 3653, 3675
AoxI GGCC 8 cut(s) 537, 681, 843, 2295, 2385, 3017, 3115, 3783
ApeKI GCWGC 2 cut(s) 12, 2143
AseI ATTAAT 2 cut(s) 2414, 3573
Asp700I GAANNNNTTC 3 cut(s) 42, 2157, 3195
Asp718I GGTACC 1 cut(s) 1657
AspS9I GGNCC 3 cut(s) 681, 2386, 3466
AsuC2I CCSGG 1 cut(s) 2857
AsuHPI GGTGA 6 cut(s) 57, 115, 680, 1481, 1816, 3548
AsuII TTCGAA 1 cut(s) 930
AvaII GGWCC 1 cut(s) 3466
BaeGI GKGCMC 2 cut(s) 1036, 3379
BamHI GGATCC 3 cut(s) 1474, 1638, 2803
BanI GGYRCC 1 cut(s) 1657
BauI CACGAG 1 cut(s) 3168
BbsI GAAGAC 3 cut(s) 779, 1240, 3286
BbvI GCAGC 2 cut(s) 24, 2155
BceAI ACGGC 1 cut(s) 1918
BciT130I CCWGG 4 cut(s) 1610, 2076, 2285, 3550
BciVI GTATCC 2 cut(s) 1594, 2586
BclI TGATCA 1 cut(s) 751
BcnI CCSGG 1 cut(s) 2857
BcoDI GTCTC 4 cut(s) 1973, 3613, 3653, 3675
BfaI CTAG 9 cut(s) 447, 942, 1643, 1865, 2207, 2360, 2507, 3696, 3765
BfmI CTRYAG 1 cut(s) 2570
BfrI CTTAAG 1 cut(s) 3434
BfuAI ACCTGC 1 cut(s) 3524
BfuI GTATCC 2 cut(s) 1594, 2586
BglII AGATCT 1 cut(s) 3430
BisI GCNGC 2 cut(s) 13, 2144
BlsI GCNGC 2 cut(s) 14, 2145
Bme1390I CCNGG 5 cut(s) 1610, 2076, 2285, 2857, 3550
Bme18I GGWCC 1 cut(s) 3466
BmeRI GACNNNNNGTC 1 cut(s) 2182
BmgT120I GGNCC 3 cut(s) 681, 2386, 3466
BmiI GGNNCC 7 cut(s) 375, 1476, 1640, 1659, 2388, 2805, 3683
BmrFI CCNGG 5 cut(s) 1610, 2076, 2285, 2857, 3550
BmrI ACTGGG 3 cut(s) 253, 416, 1644
BmsI GCATC 5 cut(s) 1808, 1969, 2289, 2593, 3453
BmuI ACTGGG 3 cut(s) 253, 416, 1644
BpiI GAAGAC 3 cut(s) 779, 1240, 3286
BpmI CTGGAG 2 cut(s) 1195, 3410
Bpu10I CCTNAGC 1 cut(s) 359
Bpu14I TTCGAA 1 cut(s) 930
BpuEI CTTGAG 3 cut(s) 2044, 2200, 3055
BpuMI CCSGG 1 cut(s) 2857
BsaBI GATNNNNATC 1 cut(s) 3429
BsaI GGTCTC 2 cut(s) 3613, 3675
BsaJI CCNNGG 6 cut(s) 1444, 1815, 2075, 3084, 3379, 3780
BsaWI WCCGGW 1 cut(s) 1740
BsaXI ACNNNNNCTCC 6 cut(s) 1168, 1198, 1283, 1313, 3712, 3742
Bsc4I CCNNNNNNNGG 4 cut(s) 319, 2395, 2728, 3792
Bse1I ACTGG 3 cut(s) 259, 411, 1639
Bse3DI GCAATG 3 cut(s) 40, 725, 3633
Bse8I GATNNNNATC 1 cut(s) 3429
BseBI CCWGG 4 cut(s) 1610, 2076, 2285, 3550
BseDI CCNNGG 6 cut(s) 1444, 1815, 2075, 3084, 3379, 3780
BseGI GGATG 7 cut(s) 158, 886, 1799, 2280, 2298, 2404, 3213
BseJI GATNNNNATC 1 cut(s) 3429
BseLI CCNNNNNNNGG 4 cut(s) 319, 2395, 2728, 3792
BseMI GCAATG 3 cut(s) 40, 725, 3633
BseMII CTCAG 7 cut(s) 533, 1011, 2435, 2772, 3405, 3600, 3624
BseNI ACTGG 3 cut(s) 259, 411, 1639
BseSI GKGCMC 2 cut(s) 1036, 3379
BseXI GCAGC 2 cut(s) 24, 2155
BseYI CCCAGC 2 cut(s) 1049, 1310
BsgI GTGCAG 2 cut(s) 2162, 3552
BshFI GGCC 8 cut(s) 539, 683, 845, 2297, 2387, 3019, 3117, 3785
BshNI GGYRCC 1 cut(s) 1657
BsiSI CCGG 3 cut(s) 1741, 2856, 3118
BslFI GGGAC 4 cut(s) 1102, 1357, 1761, 3470
BslI CCNNNNNNNGG 4 cut(s) 319, 2395, 2728, 3792
BsmAI GTCTC 4 cut(s) 1973, 3613, 3653, 3675
BsmFI GGGAC 4 cut(s) 1102, 1357, 1761, 3470
BsmI GAATGC 4 cut(s) 1066, 1694, 2041, 2157
BsnI GGCC 8 cut(s) 539, 683, 845, 2297, 2387, 3019, 3117, 3785
Bso31I GGTCTC 2 cut(s) 3613, 3675
Bsp119I TTCGAA 1 cut(s) 930
Bsp1286I GDGCHC 2 cut(s) 1036, 3379
Bsp1407I TGTACA 2 cut(s) 2231, 2991
Bsp19I CCATGG 1 cut(s) 1444
BspACI CCGC 5 cut(s) 364, 1278, 1478, 2722, 3028
BspANI GGCC 8 cut(s) 539, 683, 845, 2297, 2387, 3019, 3117, 3785
BspCNI CTCAG 7 cut(s) 532, 1012, 2434, 2773, 3406, 3599, 3623
BspHI TCATGA 2 cut(s) 1164, 3124
BspLI GGNNCC 7 cut(s) 375, 1476, 1640, 1659, 2388, 2805, 3683
BspMI ACCTGC 1 cut(s) 3524
BspT104I TTCGAA 1 cut(s) 930
BspT107I GGYRCC 1 cut(s) 1657
BspTI CTTAAG 1 cut(s) 3434
BspTNI GGTCTC 2 cut(s) 3613, 3675
BsrDI GCAATG 3 cut(s) 40, 725, 3633
BsrGI TGTACA 2 cut(s) 2231, 2991
BsrI ACTGG 3 cut(s) 259, 411, 1639
BssECI CCNNGG 6 cut(s) 1444, 1815, 2075, 3084, 3379, 3780
BssSI CACGAG 1 cut(s) 3168
BssT1I CCWWGG 5 cut(s) 1444, 1815, 3084, 3379, 3780
Bst2BI CACGAG 1 cut(s) 3168
Bst2UI CCWGG 4 cut(s) 1610, 2076, 2285, 3550
Bst4CI ACNGT 5 cut(s) 638, 2356, 2366, 3264, 3544
Bst6I CTCTTC 3 cut(s) 125, 1468, 1526
BstAFI CTTAAG 1 cut(s) 3434
BstAPI GCANNNNNTGC 1 cut(s) 3628
BstAUI TGTACA 2 cut(s) 2231, 2991
BstBI TTCGAA 1 cut(s) 930
BstC8I GCNNGC 8 cut(s) 1308, 1384, 1441, 2041, 2245, 2373, 3396, 3624
BstDSI CCRYGG 1 cut(s) 1444
BstEII GGTNACC 2 cut(s) 1150, 2070
BstF5I GGATG 7 cut(s) 158, 886, 1799, 2280, 2298, 2404, 3213
BstMAI GTCTC 4 cut(s) 1973, 3613, 3653, 3675
BstMWI GCNNNNNNNGC 4 cut(s) 427, 718, 727, 3628
BstNI CCWGG 4 cut(s) 1610, 2076, 2285, 3550
BstNSI RCATGY 1 cut(s) 1310
BstPI GGTNACC 2 cut(s) 1150, 2070
BstSCI CCNGG 5 cut(s) 1608, 2074, 2283, 2855, 3548
BstSFI CTRYAG 1 cut(s) 2570
BstSLI GKGCMC 2 cut(s) 1036, 3379
BstV1I GCAGC 2 cut(s) 24, 2155
BstV2I GAAGAC 3 cut(s) 779, 1240, 3286
BstX2I RGATCY 4 cut(s) 1474, 1638, 2803, 3430
BstYI RGATCY 4 cut(s) 1474, 1638, 2803, 3430
BsuI GTATCC 2 cut(s) 1594, 2586
BsuRI GGCC 8 cut(s) 539, 683, 845, 2297, 2387, 3019, 3117, 3785
BtgI CCRYGG 1 cut(s) 1444
BtsCI GGATG 7 cut(s) 158, 886, 1799, 2280, 2298, 2404, 3213
BtsI GCAGTG 4 cut(s) 428, 737, 1986, 2145
BtsIMutI CAGTG 9 cut(s) 428, 643, 737, 1632, 1986, 2145, 2361, 3379, 3549
BveI ACCTGC 1 cut(s) 3524
Cac8I GCNNGC 8 cut(s) 1308, 1384, 1441, 2041, 2245, 2373, 3396, 3624
CciI TCATGA 2 cut(s) 1164, 3124
Cfr13I GGNCC 3 cut(s) 681, 2386, 3466
Csp6I GTAC 6 cut(s) 57, 1257, 1658, 2232, 2942, 2992
CviQI GTAC 6 cut(s) 57, 1257, 1658, 2232, 2942, 2992
DraI TTTAAA 1 cut(s) 957
DrdI GACNNNNNNGTC 2 cut(s) 3464, 3667
DriI GACNNNNNGTC 1 cut(s) 2182
DseDI GACNNNNNNGTC 2 cut(s) 3464, 3667
Eam1104I CTCTTC 3 cut(s) 125, 1468, 1526
Eam1105I GACNNNNNGTC 1 cut(s) 2182
EarI CTCTTC 3 cut(s) 125, 1468, 1526
Eco130I CCWWGG 5 cut(s) 1444, 1815, 3084, 3379, 3780
Eco31I GGTCTC 2 cut(s) 3613, 3675
Eco32I GATATC 1 cut(s) 2166
Eco47I GGWCC 1 cut(s) 3466
Eco57I CTGAAG 2 cut(s) 1449, 3338
Eco91I GGTNACC 2 cut(s) 1150, 2070
EcoO65I GGTNACC 2 cut(s) 1150, 2070
EcoRII CCWGG 4 cut(s) 1608, 2074, 2283, 3548
EcoRV GATATC 1 cut(s) 2166
EcoT14I CCWWGG 5 cut(s) 1444, 1815, 3084, 3379, 3780
EcoT22I ATGCAT 2 cut(s) 2518, 2770
ErhI CCWWGG 5 cut(s) 1444, 1815, 3084, 3379, 3780
FaqI GGGAC 4 cut(s) 1102, 1357, 1761, 3470
FauI CCCGC 2 cut(s) 357, 1271
FbaI TGATCA 1 cut(s) 751
FblI GTMKAC 1 cut(s) 1361
Fnu4HI GCNGC 2 cut(s) 13, 2144
FokI GGATG 7 cut(s) 145, 893, 1786, 2267, 2305, 2411, 3220
Fsp4HI GCNGC 2 cut(s) 13, 2144
FspBI CTAG 9 cut(s) 447, 942, 1643, 1865, 2207, 2360, 2507, 3696, 3765
GluI GCNGC 2 cut(s) 13, 2144
GsaI CCCAGC 2 cut(s) 1053, 1314
GsuI CTGGAG 2 cut(s) 1195, 3410
HaeIII GGCC 8 cut(s) 539, 683, 845, 2297, 2387, 3019, 3117, 3785
HapII CCGG 3 cut(s) 1741, 2856, 3118
HincII GTYRAC 3 cut(s) 1362, 2458, 3241
HindII GTYRAC 3 cut(s) 1362, 2458, 3241
HindIII AAGCTT 3 cut(s) 1844, 2213, 3740
HpaI GTTAAC 1 cut(s) 2458
HpaII CCGG 3 cut(s) 1741, 2856, 3118
HphI GGTGA 6 cut(s) 57, 115, 680, 1481, 1816, 3548
Hpy166II GTNNAC 8 cut(s) 57, 65, 1187, 1213, 1362, 2458, 2994, 3241
Hpy8I GTNNAC 8 cut(s) 57, 65, 1187, 1213, 1362, 2458, 2994, 3241
HpyCH4III ACNGT 5 cut(s) 638, 2356, 2366, 3264, 3544
HpyF10VI GCNNNNNNNGC 4 cut(s) 427, 718, 727, 3628
KpnI GGTACC 1 cut(s) 1661
Ksp22I TGATCA 1 cut(s) 751
KspAI GTTAAC 1 cut(s) 2458
LmnI GCTCC 7 cut(s) 373, 1176, 2029, 2668, 2887, 3391, 3720
Lsp1109I GCAGC 2 cut(s) 24, 2155
LweI GCATC 5 cut(s) 1808, 1969, 2289, 2593, 3453
MaeI CTAG 9 cut(s) 447, 942, 1643, 1865, 2207, 2360, 2507, 3696, 3765
MaeIII GTNAC 8 cut(s) 1150, 1522, 1630, 2070, 2548, 2923, 3544, 3698
MfeI CAATTG 3 cut(s) 214, 755, 779
MflI RGATCY 4 cut(s) 1474, 1638, 2803, 3430
MhlI GDGCHC 2 cut(s) 1036, 3379
MlyI GAGTC 3 cut(s) 467, 1638, 3103
MmeI TCCRAC 5 cut(s) 415, 597, 1800, 2061, 3402
Mph1103I ATGCAT 2 cut(s) 2518, 2770
MroXI GAANNNNTTC 3 cut(s) 42, 2157, 3195
MslI CAYNNNNRTG 1 cut(s) 730
MspA1I CMGCKG 1 cut(s) 1278
MspCI CTTAAG 1 cut(s) 3434
MspI CCGG 3 cut(s) 1741, 2856, 3118
MspR9I CCNGG 5 cut(s) 1610, 2076, 2285, 2857, 3550
MunI CAATTG 3 cut(s) 214, 755, 779
Mva1269I GAATGC 4 cut(s) 1066, 1694, 2041, 2157
MvaI CCWGG 4 cut(s) 1610, 2076, 2285, 3550
MwoI GCNNNNNNNGC 4 cut(s) 427, 718, 727, 3628
NciI CCSGG 1 cut(s) 2857
NcoI CCATGG 1 cut(s) 1444
NlaIV GGNNCC 7 cut(s) 375, 1476, 1640, 1659, 2388, 2805, 3683
NmuCI GTSAC 4 cut(s) 1522, 1630, 3544, 3698
NsiI ATGCAT 2 cut(s) 2518, 2770
NspI RCATGY 1 cut(s) 1310
NspV TTCGAA 1 cut(s) 930
PaeI GCATGC 1 cut(s) 1310
PagI TCATGA 2 cut(s) 1164, 3124
PaqCI CACCTGC 1 cut(s) 3524
PctI GAATGC 4 cut(s) 1066, 1694, 2041, 2157
PdmI GAANNNNTTC 3 cut(s) 42, 2157, 3195
PfoI TCCNGGA 1 cut(s) 2283
PkrI GCNGC 2 cut(s) 14, 2145
PleI GAGTC 3 cut(s) 466, 1637, 3102
PpsI GAGTC 3 cut(s) 466, 1637, 3102
PshBI ATTAAT 2 cut(s) 2414, 3573
PsiI TTATAA 1 cut(s) 3047
Psp6I CCWGG 4 cut(s) 1608, 2074, 2283, 3548
PspEI GGTNACC 2 cut(s) 1150, 2070
PspFI CCCAGC 2 cut(s) 1049, 1310
PspGI CCWGG 4 cut(s) 1608, 2074, 2283, 3548
PspN4I GGNNCC 7 cut(s) 375, 1476, 1640, 1659, 2388, 2805, 3683
PspPI GGNCC 3 cut(s) 681, 2386, 3466
PsuI RGATCY 4 cut(s) 1474, 1638, 2803, 3430
RsaI GTAC 6 cut(s) 58, 1258, 1659, 2233, 2943, 2993
RsaNI GTAC 6 cut(s) 57, 1257, 1658, 2232, 2942, 2992
RseI CAYNNNNRTG 1 cut(s) 730
SalI GTCGAC 1 cut(s) 1360
SatI GCNGC 2 cut(s) 13, 2144
Sau96I GGNCC 3 cut(s) 681, 2386, 3466
SchI GAGTC 3 cut(s) 467, 1638, 3103
ScrFI CCNGG 5 cut(s) 1610, 2076, 2285, 2857, 3550
SduI GDGCHC 2 cut(s) 1036, 3379
SfaNI GCATC 5 cut(s) 1808, 1969, 2289, 2593, 3453
SfcI CTRYAG 1 cut(s) 2570
SfuI TTCGAA 1 cut(s) 930
SinI GGWCC 1 cut(s) 3466
SmiMI CAYNNNNRTG 1 cut(s) 730
SmlI CTYRAG 4 cut(s) 2023, 2179, 3034, 3434
SmoI CTYRAG 4 cut(s) 2023, 2179, 3034, 3434
SphI GCATGC 1 cut(s) 1310
SsiI CCGC 5 cut(s) 364, 1278, 1478, 2722, 3028
SspI AATATT 2 cut(s) 2050, 2830
SspMI CTAG 9 cut(s) 447, 942, 1643, 1865, 2207, 2360, 2507, 3696, 3765
StyD4I CCNGG 5 cut(s) 1608, 2074, 2283, 2855, 3548
StyI CCWWGG 5 cut(s) 1444, 1815, 3084, 3379, 3780
TaaI ACNGT 5 cut(s) 638, 2356, 2366, 3264, 3544
TaqI TCGA 8 cut(s) 700, 930, 1361, 2972, 3097, 3447, 3481, 3657
TaqII GACCGA 1 cut(s) 3298
TatI WGTACW 3 cut(s) 2231, 2941, 2991
TscAI CASTG 9 cut(s) 428, 643, 737, 1639, 1993, 2145, 2361, 3379, 3549
TseFI GTSAC 4 cut(s) 1522, 1630, 3544, 3698
TseI GCWGC 2 cut(s) 12, 2143
Tsp45I GTSAC 4 cut(s) 1522, 1630, 3544, 3698
TspRI CASTG 9 cut(s) 428, 643, 737, 1639, 1993, 2145, 2361, 3379, 3549
Vha464I CTTAAG 1 cut(s) 3434
VpaK11BI GGWCC 1 cut(s) 3466
VspI ATTAAT 2 cut(s) 2414, 3573
XbaI TCTAGA 1 cut(s) 2506
XceI RCATGY 1 cut(s) 1310
XcmI CCANNNNNNNNNTGG 1 cut(s) 1492
XmiI GTMKAC 1 cut(s) 1361
XmnI GAANNNNTTC 3 cut(s) 42, 2157, 3195
XspI CTAG 9 cut(s) 447, 942, 1643, 1865, 2207, 2360, 2507, 3696, 3765
Zsp2I ATGCAT 2 cut(s) 2518, 2770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.