Rh2BG676000

B2 protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002574
Physical Location & Seq
Forward (+)
88720423 .. 88722183
1761 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG676000.1

Sequence Viewer

Length: 906 bp
ATGGAGAGCATCCATACCTATTGGCAGTTGGGTGATGAGCTTCGTGGACAGTCGAAAGTTGCAGAGGATCACAAATGGTTAATGGTTGCTTCCAAATTGGCTGAGCAAACAAGGTCAAAGGGCGAGCGCATGAATAACCTTGATCTTTCAAAGGGCCCAACTGAAACAAGAGCAAGGGACAAATTTGGGTTCCAGGAAGATAACAAATTTGAAAGCCTTAACTTTAACATGTTGAACTTGGACTCGAAAGTAAATGAAAATGTGAGCAAGAGTTCCTTTAGGAATGGGATTTACAACATGAATGCAGTTTACCAGAAAAACAGTGCAAGCTTTGTGGGGAACATGACCGGTAACAAGTACAATGGCAATCACCTCAGCAACAAGGAAACCAACAACAGCGGTAATAACAACAACAACAACAACGAAACTGGGAATGTTGTTGAGAAAAGGTTCAAGACCTTGCCTGCAACTGAGACACTTCCCCGAAATGAGGTGCTTGGAGGTTACATCTTTGTATGCAACAATGACACAATGCAGGAAGATTTGAAGCGACAACTATTCGGTTTACCTCCAAGGTACAGGGACTCTGTTCGGGCGATTACACCTGGCTTACCTCTGTTTCTCTACAACTATACAACCCACCAACTGCATGGCATTTTTGAGGCATCAAGCTTTGGCGGTTCAAACATTGATCCAACTGCTTGGGAAGACAAGAAATGTAAAGGCGAGTCAAGGTTTCCTGCGCAGGTAAGGATCTGTGTTAGAAAAATCTGCAAGGCTCTGGAAGAAGATTCCTTCAGGCCAGTCTTGCATCACTATGATGGTCCAAAGTTCCGTCTTGAGTTGTCAGTTCCCGAGACCCTGGATCTATTGGACCTATGTGAGCAAGCCGGCTCTGCAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

301

Amino Acids

34.15

Weight (kDa)

7.05

Isoelectric Point (pI)

33.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 167 - 293 2.7e-39 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 744
Acc36I ACCTGC 1 cut(s) 736
AciI CCGC 2 cut(s) 399, 678
AclWI GGATC 4 cut(s) 75, 686, 761, 873
AcsI RAATTY 2 cut(s) 182, 206
AcuI CTGAAG 1 cut(s) 781
AfaI GTAC 2 cut(s) 359, 578
AfiI CCNNNNNNNGG 1 cut(s) 490
AflIII ACRYGT 1 cut(s) 228
AgeI ACCGGT 1 cut(s) 347
AgsI TTSAA 6 cut(s) 150, 212, 235, 454, 547, 684
AjnI CCWGG 3 cut(s) 192, 604, 861
AluBI AGCT 3 cut(s) 40, 330, 672
AluI AGCT 3 cut(s) 40, 330, 672
Alw26I GTCTC 2 cut(s) 467, 851
AlwI GGATC 4 cut(s) 75, 686, 761, 873
Ama87I CYCGRG 1 cut(s) 854
AoxI GGCC 2 cut(s) 154, 800
ApaI GGGCCC 1 cut(s) 158
ApeKI GCWGC 1 cut(s) 899
ApoI RAATTY 2 cut(s) 182, 206
AsiGI ACCGGT 1 cut(s) 347
Asp700I GAANNNNTTC 1 cut(s) 449
AspLEI GCGC 2 cut(s) 129, 745
AspS9I GGNCC 4 cut(s) 154, 155, 824, 874
AsuHPI GGTGA 2 cut(s) 44, 362
AvaI CYCGRG 1 cut(s) 854
AvaII GGWCC 2 cut(s) 824, 874
BaeGI GKGCMC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 158
BbsI GAAGAC 1 cut(s) 714
BbvCI CCTCAGC 1 cut(s) 374
BccI CCATC 1 cut(s) 815
BciT130I CCWGG 3 cut(s) 194, 606, 863
BcoDI GTCTC 2 cut(s) 467, 851
BfmI CTRYAG 1 cut(s) 897
BfuAI ACCTGC 1 cut(s) 736
BisI GCNGC 1 cut(s) 900
BlpI GCTNAGC 1 cut(s) 102
BlsI GCNGC 1 cut(s) 901
Bme1390I CCNGG 3 cut(s) 194, 606, 863
Bme18I GGWCC 2 cut(s) 824, 874
BmeT110I CYCGRG 1 cut(s) 854
BmgT120I GGNCC 4 cut(s) 154, 155, 824, 874
BmiI GGNNCC 2 cut(s) 156, 191
BmrFI CCNGG 3 cut(s) 194, 606, 863
BmrI ACTGGG 1 cut(s) 438
BmsI GCATC 3 cut(s) 18, 674, 820
BmuI ACTGGG 1 cut(s) 438
BpiI GAAGAC 1 cut(s) 714
Bpu10I CCTNAGC 1 cut(s) 374
Bpu1102I GCTNAGC 1 cut(s) 102
BpuEI CTTGAG 1 cut(s) 860
BsaI GGTCTC 1 cut(s) 851
BsaJI CCNNGG 2 cut(s) 572, 861
BsaWI WCCGGW 1 cut(s) 347
Bsc4I CCNNNNNNNGG 1 cut(s) 490
Bse118I RCCGGY 2 cut(s) 347, 890
Bse1I ACTGG 2 cut(s) 433, 803
BseBI CCWGG 3 cut(s) 194, 606, 863
BseDI CCNNGG 2 cut(s) 572, 861
BseGI GGATG 1 cut(s) 9
BseLI CCNNNNNNNGG 1 cut(s) 490
BseMII CTCAG 3 cut(s) 93, 388, 462
BseNI ACTGG 2 cut(s) 433, 803
BseSI GKGCMC 1 cut(s) 158
BshFI GGCC 2 cut(s) 156, 802
BshTI ACCGGT 1 cut(s) 347
BsiHKCI CYCGRG 1 cut(s) 854
BsiSI CCGG 2 cut(s) 348, 891
BslFI GGGAC 2 cut(s) 191, 596
BslI CCNNNNNNNGG 1 cut(s) 490
BsmAI GTCTC 2 cut(s) 467, 851
BsmFI GGGAC 2 cut(s) 191, 596
BsmI GAATGC 1 cut(s) 307
BsnI GGCC 2 cut(s) 156, 802
Bso31I GGTCTC 1 cut(s) 851
BsoBI CYCGRG 1 cut(s) 854
Bsp120I GGGCCC 1 cut(s) 154
Bsp1286I GDGCHC 1 cut(s) 158
Bsp143I GATC 5 cut(s) 67, 142, 691, 753, 865
Bsp1720I GCTNAGC 1 cut(s) 102
BspACI CCGC 2 cut(s) 399, 678
BspANI GGCC 2 cut(s) 156, 802
BspCNI CTCAG 3 cut(s) 94, 387, 463
BspLI GGNNCC 2 cut(s) 156, 191
BspMAI CTGCAG 1 cut(s) 901
BspMI ACCTGC 1 cut(s) 736
BspPI GGATC 4 cut(s) 75, 686, 761, 873
BspTNI GGTCTC 1 cut(s) 851
BsrFI RCCGGY 2 cut(s) 347, 890
BsrI ACTGG 2 cut(s) 433, 803
BssAI RCCGGY 2 cut(s) 347, 890
BssECI CCNNGG 2 cut(s) 572, 861
BssMI GATC 5 cut(s) 67, 142, 691, 753, 865
BssT1I CCWWGG 1 cut(s) 572
Bst2UI CCWGG 3 cut(s) 194, 606, 863
Bst4CI ACNGT 2 cut(s) 51, 323
BstC8I GCNNGC 5 cut(s) 125, 328, 465, 888, 892
BstDEI CTNAG 3 cut(s) 102, 374, 471
BstF5I GGATG 1 cut(s) 9
BstHHI GCGC 2 cut(s) 129, 745
BstKTI GATC 5 cut(s) 70, 145, 694, 756, 868
BstMAI GTCTC 2 cut(s) 467, 851
BstMBI GATC 5 cut(s) 67, 142, 691, 753, 865
BstMWI GCNNNNNNNGC 2 cut(s) 808, 896
BstNI CCWGG 3 cut(s) 194, 606, 863
BstNSI RCATGY 1 cut(s) 232
BstSCI CCNGG 3 cut(s) 192, 604, 861
BstSFI CTRYAG 1 cut(s) 897
BstSLI GKGCMC 1 cut(s) 158
BstV2I GAAGAC 1 cut(s) 714
BstX2I RGATCY 2 cut(s) 753, 865
BstXI CCANNNNNNTGG 2 cut(s) 650, 702
BstYI RGATCY 2 cut(s) 753, 865
BsuRI GGCC 2 cut(s) 156, 802
BtsCI GGATG 1 cut(s) 9
BtsIMutI CAGTG 1 cut(s) 328
BveI ACCTGC 1 cut(s) 736
Cac8I GCNNGC 5 cut(s) 125, 328, 465, 888, 892
CfoI GCGC 2 cut(s) 129, 745
Cfr10I RCCGGY 2 cut(s) 347, 890
Cfr13I GGNCC 4 cut(s) 154, 155, 824, 874
Csp6I GTAC 2 cut(s) 358, 577
CspAI ACCGGT 1 cut(s) 347
CspCI CAANNNNNGTGG 2 cut(s) 315, 350
CviAII CATG 5 cut(s) 130, 229, 298, 343, 650
CviQI GTAC 2 cut(s) 358, 577
DdeI CTNAG 3 cut(s) 102, 374, 471
DpnI GATC 5 cut(s) 69, 144, 693, 755, 867
DpnII GATC 5 cut(s) 67, 142, 691, 753, 865
Eco130I CCWWGG 1 cut(s) 572
Eco24I GRGCYC 1 cut(s) 158
Eco31I GGTCTC 1 cut(s) 851
Eco47I GGWCC 2 cut(s) 824, 874
Eco57I CTGAAG 1 cut(s) 781
Eco88I CYCGRG 1 cut(s) 854
EcoO109I RGGNCCY 1 cut(s) 154
EcoRII CCWGG 3 cut(s) 192, 604, 861
EcoT14I CCWWGG 1 cut(s) 572
EcoT38I GRGCYC 1 cut(s) 158
ErhI CCWWGG 1 cut(s) 572
FaeI CATG 5 cut(s) 133, 232, 301, 346, 653
FalI AAGNNNNNCTT 2 cut(s) 260, 292
FaqI GGGAC 2 cut(s) 191, 596
FatI CATG 5 cut(s) 129, 228, 297, 342, 649
Fnu4HI GCNGC 1 cut(s) 900
FriOI GRGCYC 1 cut(s) 158
Fsp4HI GCNGC 1 cut(s) 900
FspI TGCGCA 1 cut(s) 744
GlaI GCGC 2 cut(s) 128, 744
GluI GCNGC 1 cut(s) 900
HaeIII GGCC 2 cut(s) 156, 802
HapII CCGG 2 cut(s) 348, 891
HhaI GCGC 2 cut(s) 129, 745
Hin1II CATG 5 cut(s) 133, 232, 301, 346, 653
Hin6I GCGC 2 cut(s) 127, 743
HinP1I GCGC 2 cut(s) 127, 743
HindIII AAGCTT 2 cut(s) 328, 670
HinfI GANTC 4 cut(s) 242, 584, 728, 791
HpaII CCGG 2 cut(s) 348, 891
HphI GGTGA 2 cut(s) 44, 362
Hpy166II GTNNAC 3 cut(s) 47, 310, 566
Hpy188III TCNNGA 4 cut(s) 454, 782, 839, 854
Hpy8I GTNNAC 3 cut(s) 47, 310, 566
HpyAV CCTTC 1 cut(s) 805
HpyCH4III ACNGT 2 cut(s) 51, 323
HpyF10VI GCNNNNNNNGC 2 cut(s) 808, 896
HpyF3I CTNAG 3 cut(s) 102, 374, 471
Hsp92II CATG 5 cut(s) 133, 232, 301, 346, 653
HspAI GCGC 2 cut(s) 127, 743
KroI GCCGGC 1 cut(s) 890
KroNI GCCGGC 1 cut(s) 892
Kzo9I GATC 5 cut(s) 67, 142, 691, 753, 865
LweI GCATC 3 cut(s) 18, 674, 820
MaeIII GTNAC 2 cut(s) 350, 503
MalI GATC 5 cut(s) 69, 144, 693, 755, 867
MboI GATC 5 cut(s) 67, 142, 691, 753, 865
MboII GAAGA 5 cut(s) 209, 551, 719, 797, 800
MflI RGATCY 2 cut(s) 753, 865
MhlI GDGCHC 1 cut(s) 158
MluCI AATT 3 cut(s) 95, 182, 206
MlyI GAGTC 3 cut(s) 236, 578, 737
MmeI TCCRAC 1 cut(s) 719
MnlI CCTC 7 cut(s) 58, 383, 484, 494, 579, 624, 655
MroNI GCCGGC 1 cut(s) 890
MroXI GAANNNNTTC 1 cut(s) 449
MseI TTAA 3 cut(s) 80, 219, 225
MslI CAYNNNNRTG 2 cut(s) 816, 819
MspA1I CMGCKG 1 cut(s) 399
MspI CCGG 2 cut(s) 348, 891
MspR9I CCNGG 3 cut(s) 194, 606, 863
Mva1269I GAATGC 1 cut(s) 307
MvaI CCWGG 3 cut(s) 194, 606, 863
MwoI GCNNNNNNNGC 2 cut(s) 808, 896
NaeI GCCGGC 1 cut(s) 892
NdeII GATC 5 cut(s) 67, 142, 691, 753, 865
NgoMIV GCCGGC 1 cut(s) 890
NlaIII CATG 5 cut(s) 133, 232, 301, 346, 653
NlaIV GGNNCC 2 cut(s) 156, 191
NsbI TGCGCA 1 cut(s) 744
NspI RCATGY 1 cut(s) 232
PciI ACATGT 1 cut(s) 228
PctI GAATGC 1 cut(s) 307
PdiI GCCGGC 1 cut(s) 892
PdmI GAANNNNTTC 1 cut(s) 449
PfeI GAWTC 1 cut(s) 791
PfoI TCCNGGA 1 cut(s) 192
PinAI ACCGGT 1 cut(s) 347
PkrI GCNGC 1 cut(s) 901
PleI GAGTC 3 cut(s) 236, 578, 736
PpsI GAGTC 3 cut(s) 236, 578, 736
PscI ACATGT 1 cut(s) 228
Psp6I CCWGG 3 cut(s) 192, 604, 861
PspGI CCWGG 3 cut(s) 192, 604, 861
PspN4I GGNNCC 2 cut(s) 156, 191
PspOMI GGGCCC 1 cut(s) 154
PspPI GGNCC 4 cut(s) 154, 155, 824, 874
PstI CTGCAG 1 cut(s) 901
PsuI RGATCY 2 cut(s) 753, 865
RsaI GTAC 2 cut(s) 359, 578
RsaNI GTAC 2 cut(s) 358, 577
RseI CAYNNNNRTG 2 cut(s) 816, 819
SaqAI TTAA 3 cut(s) 80, 219, 225
SatI GCNGC 1 cut(s) 900
Sau3AI GATC 5 cut(s) 67, 142, 691, 753, 865
Sau96I GGNCC 4 cut(s) 154, 155, 824, 874
SchI GAGTC 3 cut(s) 236, 578, 737
ScrFI CCNGG 3 cut(s) 194, 606, 863
SduI GDGCHC 1 cut(s) 158
SfaNI GCATC 3 cut(s) 18, 674, 820
SfcI CTRYAG 1 cut(s) 897
SinI GGWCC 2 cut(s) 824, 874
SmiMI CAYNNNNRTG 2 cut(s) 816, 819
SmlI CTYRAG 1 cut(s) 839
SmoI CTYRAG 1 cut(s) 839
Sse9I AATT 3 cut(s) 95, 182, 206
SsiI CCGC 2 cut(s) 399, 678
StyD4I CCNGG 3 cut(s) 192, 604, 861
StyI CCWWGG 1 cut(s) 572
TaaI ACNGT 2 cut(s) 51, 323
TaqI TCGA 2 cut(s) 53, 245
TasI AATT 3 cut(s) 95, 182, 206
TatI WGTACW 1 cut(s) 357
TfiI GAWTC 1 cut(s) 791
Tru1I TTAA 3 cut(s) 80, 219, 225
Tru9I TTAA 3 cut(s) 80, 219, 225
TscAI CASTG 1 cut(s) 328
TseI GCWGC 1 cut(s) 899
TspDTI ATGAA 3 cut(s) 146, 270, 314
TspGWI ACGGA 1 cut(s) 824
TspRI CASTG 1 cut(s) 328
VpaK11BI GGWCC 2 cut(s) 824, 874
XapI RAATTY 2 cut(s) 182, 206
XceI RCATGY 1 cut(s) 232
XcmI CCANNNNNNNNNTGG 1 cut(s) 647
XmnI GAANNNNTTC 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.