RLG00000022337

B2 protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002574
Physical Location & Seq
Forward (+)
82675142 .. 82676906
1765 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022337

Sequence Viewer

Length: 909 bp
ATGGAGAGCATCCATACCTATTGGCAGTTGGGTGATGAGCTTCGTGGACAGTCGAAAGTTGCAGAGGATCACAAATGGTTAATGGTTGCCTCCAAATTGGCTGAGCAAACGAGGTCAAAGGGCGAGCGCATGAATAATCTTGATCTTTCAAAGGGCCCAACTGAAACAAGAGCGAGGGACAAATTTGGGTTCCAGGAAGATAACAAATTTGAAAGCCTTAACTTTAACATGTTGAACTTGGACTCGAAAGTAAATGAAAATGTGAGCAAGAGTTCCTTTAGGAATGGGATTTACAACATGAATGCAATTTACCAGAAAAACAGTGCAAGCTTTGTGGGGAACATGACTGGTAACAAGTACAATGGCAATCACCTCAGCAACAAGGAAACCAACAACAGCGGTAACAACAACAACAACAACAGCAACGAAACTGGGAATGTTGTTGAGAAAAGGTTCAAGACCTTGCCTGCAACTGAGACACTTCCCCGAAATGAGGTGCTTGGAGGTTACATCTTTGTATGCAACAATGACACAATGCAGGAAGATTTGAAGCGACAACTATTCGGTTTACCTCCAAGGTACAGGGATTCTGTTCGGGCGATTACACCTGGCTTGCCTCTGTTTCTCTACAACTATACAACCCACCAACTGCATGGCATTTTTGAGGCATCAAGCTTTGGCGGTTCAAACATTGATCCAACTGCTTGGGAAGACAAGAAATGTAAAGGCGAGTCAAGGTTTCCTGCACAGGTAAGGATCCGTGTTAGAAAAATCTGCAAGGCTCTGGAAGAAGATTCCTTCAGGCCAGTCTTGCATCACTATGATGGTCCAAAGTTCCGTCTTGAGTTGTCAGTTCCCGAGACCCTGGATCTATTGGACCTATGTGAGCAAGCCGGCTCTGCAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

303

Amino Acids

34.3

Weight (kDa)

7.68

Isoelectric Point (pI)

34.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 168 - 294 1.3e-39 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 399, 681
AclWI GGATC 5 cut(s) 75, 689, 751, 764, 876
AcsI RAATTY 2 cut(s) 182, 206
AcuI CTGAAG 1 cut(s) 784
AfaI GTAC 2 cut(s) 359, 581
AfiI CCNNNNNNNGG 1 cut(s) 493
AflIII ACRYGT 1 cut(s) 228
AgsI TTSAA 6 cut(s) 150, 212, 235, 457, 550, 687
AjnI CCWGG 3 cut(s) 192, 607, 864
AluBI AGCT 3 cut(s) 40, 330, 675
AluI AGCT 3 cut(s) 40, 330, 675
Alw26I GTCTC 2 cut(s) 470, 854
AlwI GGATC 5 cut(s) 75, 689, 751, 764, 876
Ama87I CYCGRG 1 cut(s) 857
AoxI GGCC 2 cut(s) 154, 803
ApaI GGGCCC 1 cut(s) 158
ApeKI GCWGC 1 cut(s) 902
ApoI RAATTY 2 cut(s) 182, 206
Asp700I GAANNNNTTC 1 cut(s) 452
AspLEI GCGC 1 cut(s) 129
AspS9I GGNCC 4 cut(s) 154, 155, 827, 877
AsuHPI GGTGA 2 cut(s) 44, 362
AvaI CYCGRG 1 cut(s) 857
AvaII GGWCC 2 cut(s) 827, 877
BaeGI GKGCMC 1 cut(s) 158
BamHI GGATCC 1 cut(s) 756
BanII GRGCYC 1 cut(s) 158
BbsI GAAGAC 1 cut(s) 717
BbvCI CCTCAGC 1 cut(s) 374
BccI CCATC 1 cut(s) 818
BciT130I CCWGG 3 cut(s) 194, 609, 866
BcoDI GTCTC 2 cut(s) 470, 854
BfmI CTRYAG 1 cut(s) 900
BisI GCNGC 1 cut(s) 903
BlpI GCTNAGC 1 cut(s) 102
BlsI GCNGC 1 cut(s) 904
Bme1390I CCNGG 3 cut(s) 194, 609, 866
Bme18I GGWCC 2 cut(s) 827, 877
BmeT110I CYCGRG 1 cut(s) 857
BmgT120I GGNCC 4 cut(s) 154, 155, 827, 877
BmiI GGNNCC 3 cut(s) 156, 191, 758
BmrFI CCNGG 3 cut(s) 194, 609, 866
BmrI ACTGGG 1 cut(s) 441
BmsI GCATC 3 cut(s) 18, 677, 823
BmuI ACTGGG 1 cut(s) 441
BpiI GAAGAC 1 cut(s) 717
Bpu10I CCTNAGC 1 cut(s) 374
Bpu1102I GCTNAGC 1 cut(s) 102
BpuEI CTTGAG 1 cut(s) 863
BsaI GGTCTC 1 cut(s) 854
BsaJI CCNNGG 2 cut(s) 575, 864
Bsc4I CCNNNNNNNGG 1 cut(s) 493
Bse118I RCCGGY 1 cut(s) 893
Bse1I ACTGG 3 cut(s) 352, 436, 806
BseBI CCWGG 3 cut(s) 194, 609, 866
BseDI CCNNGG 2 cut(s) 575, 864
BseGI GGATG 1 cut(s) 9
BseLI CCNNNNNNNGG 1 cut(s) 493
BseMII CTCAG 3 cut(s) 93, 388, 465
BseNI ACTGG 3 cut(s) 352, 436, 806
BseSI GKGCMC 1 cut(s) 158
BsgI GTGCAG 1 cut(s) 729
BshFI GGCC 2 cut(s) 156, 805
BsiHKCI CYCGRG 1 cut(s) 857
BsiSI CCGG 1 cut(s) 894
BslFI GGGAC 1 cut(s) 191
BslI CCNNNNNNNGG 1 cut(s) 493
BsmAI GTCTC 2 cut(s) 470, 854
BsmFI GGGAC 1 cut(s) 191
BsmI GAATGC 1 cut(s) 307
BsnI GGCC 2 cut(s) 156, 805
Bso31I GGTCTC 1 cut(s) 854
BsoBI CYCGRG 1 cut(s) 857
Bsp120I GGGCCC 1 cut(s) 154
Bsp1286I GDGCHC 1 cut(s) 158
Bsp143I GATC 5 cut(s) 67, 142, 694, 756, 868
Bsp1720I GCTNAGC 1 cut(s) 102
BspACI CCGC 2 cut(s) 399, 681
BspANI GGCC 2 cut(s) 156, 805
BspCNI CTCAG 3 cut(s) 94, 387, 466
BspLI GGNNCC 3 cut(s) 156, 191, 758
BspMAI CTGCAG 1 cut(s) 904
BspPI GGATC 5 cut(s) 75, 689, 751, 764, 876
BspTNI GGTCTC 1 cut(s) 854
BsrFI RCCGGY 1 cut(s) 893
BsrI ACTGG 3 cut(s) 352, 436, 806
BssAI RCCGGY 1 cut(s) 893
BssECI CCNNGG 2 cut(s) 575, 864
BssMI GATC 5 cut(s) 67, 142, 694, 756, 868
BssT1I CCWWGG 1 cut(s) 575
Bst2UI CCWGG 3 cut(s) 194, 609, 866
Bst4CI ACNGT 2 cut(s) 51, 323
BstC8I GCNNGC 6 cut(s) 125, 328, 468, 614, 891, 895
BstDEI CTNAG 3 cut(s) 102, 374, 474
BstF5I GGATG 1 cut(s) 9
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 5 cut(s) 70, 145, 697, 759, 871
BstMAI GTCTC 2 cut(s) 470, 854
BstMBI GATC 5 cut(s) 67, 142, 694, 756, 868
BstMWI GCNNNNNNNGC 2 cut(s) 811, 899
BstNI CCWGG 3 cut(s) 194, 609, 866
BstNSI RCATGY 1 cut(s) 232
BstSCI CCNGG 3 cut(s) 192, 607, 864
BstSFI CTRYAG 1 cut(s) 900
BstSLI GKGCMC 1 cut(s) 158
BstV2I GAAGAC 1 cut(s) 717
BstX2I RGATCY 2 cut(s) 756, 868
BstXI CCANNNNNNTGG 2 cut(s) 653, 705
BstYI RGATCY 2 cut(s) 756, 868
BsuRI GGCC 2 cut(s) 156, 805
BtsCI GGATG 1 cut(s) 9
BtsIMutI CAGTG 1 cut(s) 328
Cac8I GCNNGC 6 cut(s) 125, 328, 468, 614, 891, 895
CfoI GCGC 1 cut(s) 129
Cfr10I RCCGGY 1 cut(s) 893
Cfr13I GGNCC 4 cut(s) 154, 155, 827, 877
Csp6I GTAC 2 cut(s) 358, 580
CspCI CAANNNNNGTGG 2 cut(s) 315, 350
CviAII CATG 5 cut(s) 130, 229, 298, 343, 653
CviQI GTAC 2 cut(s) 358, 580
DdeI CTNAG 3 cut(s) 102, 374, 474
DpnI GATC 5 cut(s) 69, 144, 696, 758, 870
DpnII GATC 5 cut(s) 67, 142, 694, 756, 868
Eco130I CCWWGG 1 cut(s) 575
Eco24I GRGCYC 1 cut(s) 158
Eco31I GGTCTC 1 cut(s) 854
Eco47I GGWCC 2 cut(s) 827, 877
Eco57I CTGAAG 1 cut(s) 784
Eco88I CYCGRG 1 cut(s) 857
EcoO109I RGGNCCY 1 cut(s) 154
EcoRII CCWGG 3 cut(s) 192, 607, 864
EcoT14I CCWWGG 1 cut(s) 575
EcoT38I GRGCYC 1 cut(s) 158
ErhI CCWWGG 1 cut(s) 575
FaeI CATG 5 cut(s) 133, 232, 301, 346, 656
FalI AAGNNNNNCTT 2 cut(s) 260, 292
FaqI GGGAC 1 cut(s) 191
FatI CATG 5 cut(s) 129, 228, 297, 342, 652
Fnu4HI GCNGC 1 cut(s) 903
FriOI GRGCYC 1 cut(s) 158
Fsp4HI GCNGC 1 cut(s) 903
GlaI GCGC 1 cut(s) 128
GluI GCNGC 1 cut(s) 903
HaeIII GGCC 2 cut(s) 156, 805
HapII CCGG 1 cut(s) 894
HhaI GCGC 1 cut(s) 129
Hin1II CATG 5 cut(s) 133, 232, 301, 346, 656
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HindIII AAGCTT 2 cut(s) 328, 673
HinfI GANTC 4 cut(s) 242, 587, 731, 794
HpaII CCGG 1 cut(s) 894
HphI GGTGA 2 cut(s) 44, 362
Hpy166II GTNNAC 2 cut(s) 47, 569
Hpy188III TCNNGA 5 cut(s) 140, 457, 785, 842, 857
Hpy8I GTNNAC 2 cut(s) 47, 569
HpyAV CCTTC 1 cut(s) 808
HpyCH4III ACNGT 2 cut(s) 51, 323
HpyF10VI GCNNNNNNNGC 2 cut(s) 811, 899
HpyF3I CTNAG 3 cut(s) 102, 374, 474
Hsp92II CATG 5 cut(s) 133, 232, 301, 346, 656
HspAI GCGC 1 cut(s) 127
KroI GCCGGC 1 cut(s) 893
KroNI GCCGGC 1 cut(s) 895
Kzo9I GATC 5 cut(s) 67, 142, 694, 756, 868
LweI GCATC 3 cut(s) 18, 677, 823
MaeIII GTNAC 3 cut(s) 350, 401, 506
MalI GATC 5 cut(s) 69, 144, 696, 758, 870
MboI GATC 5 cut(s) 67, 142, 694, 756, 868
MboII GAAGA 5 cut(s) 209, 554, 722, 800, 803
MflI RGATCY 2 cut(s) 756, 868
MhlI GDGCHC 1 cut(s) 158
MluCI AATT 4 cut(s) 95, 182, 206, 306
MlyI GAGTC 2 cut(s) 236, 740
MmeI TCCRAC 1 cut(s) 722
MroNI GCCGGC 1 cut(s) 893
MroXI GAANNNNTTC 1 cut(s) 452
MseI TTAA 3 cut(s) 80, 219, 225
MslI CAYNNNNRTG 2 cut(s) 819, 822
MspA1I CMGCKG 1 cut(s) 399
MspI CCGG 1 cut(s) 894
MspR9I CCNGG 3 cut(s) 194, 609, 866
Mva1269I GAATGC 1 cut(s) 307
MvaI CCWGG 3 cut(s) 194, 609, 866
MwoI GCNNNNNNNGC 2 cut(s) 811, 899
NaeI GCCGGC 1 cut(s) 895
NdeII GATC 5 cut(s) 67, 142, 694, 756, 868
NgoMIV GCCGGC 1 cut(s) 893
NlaIII CATG 5 cut(s) 133, 232, 301, 346, 656
NlaIV GGNNCC 3 cut(s) 156, 191, 758
NspI RCATGY 1 cut(s) 232
PciI ACATGT 1 cut(s) 228
PctI GAATGC 1 cut(s) 307
PdiI GCCGGC 1 cut(s) 895
PdmI GAANNNNTTC 1 cut(s) 452
PfeI GAWTC 2 cut(s) 587, 794
PfoI TCCNGGA 1 cut(s) 192
PkrI GCNGC 1 cut(s) 904
PleI GAGTC 2 cut(s) 236, 739
PpsI GAGTC 2 cut(s) 236, 739
PscI ACATGT 1 cut(s) 228
Psp6I CCWGG 3 cut(s) 192, 607, 864
PspGI CCWGG 3 cut(s) 192, 607, 864
PspN4I GGNNCC 3 cut(s) 156, 191, 758
PspOMI GGGCCC 1 cut(s) 154
PspPI GGNCC 4 cut(s) 154, 155, 827, 877
PstI CTGCAG 1 cut(s) 904
PsuI RGATCY 2 cut(s) 756, 868
RsaI GTAC 2 cut(s) 359, 581
RsaNI GTAC 2 cut(s) 358, 580
RseI CAYNNNNRTG 2 cut(s) 819, 822
SaqAI TTAA 3 cut(s) 80, 219, 225
SatI GCNGC 1 cut(s) 903
Sau3AI GATC 5 cut(s) 67, 142, 694, 756, 868
Sau96I GGNCC 4 cut(s) 154, 155, 827, 877
SchI GAGTC 2 cut(s) 236, 740
ScrFI CCNGG 3 cut(s) 194, 609, 866
SduI GDGCHC 1 cut(s) 158
SfaNI GCATC 3 cut(s) 18, 677, 823
SfcI CTRYAG 1 cut(s) 900
SinI GGWCC 2 cut(s) 827, 877
SmiMI CAYNNNNRTG 2 cut(s) 819, 822
SmlI CTYRAG 1 cut(s) 842
SmoI CTYRAG 1 cut(s) 842
Sse9I AATT 4 cut(s) 95, 182, 206, 306
SsiI CCGC 2 cut(s) 399, 681
StyD4I CCNGG 3 cut(s) 192, 607, 864
StyI CCWWGG 1 cut(s) 575
TaaI ACNGT 2 cut(s) 51, 323
TaqI TCGA 2 cut(s) 53, 245
TasI AATT 4 cut(s) 95, 182, 206, 306
TatI WGTACW 1 cut(s) 357
TfiI GAWTC 2 cut(s) 587, 794
Tru1I TTAA 3 cut(s) 80, 219, 225
Tru9I TTAA 3 cut(s) 80, 219, 225
TscAI CASTG 1 cut(s) 328
TseI GCWGC 1 cut(s) 902
TspDTI ATGAA 3 cut(s) 146, 270, 314
TspGWI ACGGA 2 cut(s) 749, 827
TspRI CASTG 1 cut(s) 328
VpaK11BI GGWCC 2 cut(s) 827, 877
XapI RAATTY 2 cut(s) 182, 206
XceI RCATGY 1 cut(s) 232
XcmI CCANNNNNNNNNTGG 1 cut(s) 650
XmnI GAANNNNTTC 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.