Rroxscaffold_7G00217590

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002574
Physical Location & Seq
Forward (+)
68061733 .. 68063475
1743 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00217590.1

Sequence Viewer

Length: 942 bp
ATGTCAATGAGTAATTACAGTAAGATGAGTAGTAGCTCTGTGTGGCAGTTGTCAGTTACTACTCGTAAGTATGATGTACTACAATATCATGATGATCCAGTGGTCATAGCCTCCCGAGAAGCTGATCATGATCAGTATCAAACACAGACAGCAGCAACAAGAGTAGCACTGATTGACAATCAATTGATGCAGATGACTACTACTGCCGCAGAGTACTCAGATGATGAAGATCATAATAAGAAGGATTATTATTATCGTTATGGAAAGAGTAGTAGTTCCAATTTGAAGAAAAACAACAATATGAGTTATGCGGCATTGAAATTTTTAGAAATGAAATTAGGCGGCGTGCAGGCTGTGCAGCATAACAATAAGTCCCTTCATTACTATGTGCAGCAACAGCAGCAGCAGAGTGATAGTGAGAGTAGTGAGATGATGAGCAGTAGTCATGACAATGATTATTGTTATTACAATAAGCAGCGGCAGGTGTGGCATAAGACGCTGCCTCAGGCCGAAATGCTGCCTAGAAATGAAGTCTTGGGAGGTTACATTTTTGTATGCAACAACGAAACCATGCAAGAGGATCTCCACCGTCAACTCTTCGGACTCCCTCAAAAGTACAAGGACTCTGTCAGGGAAATTACCCCAGGCTTACCCCTTTTTCTTTACAACTACACAGCCCACCAGCTCCATGGAGTTTTTCAGGCTGTGAGTTTTGGAGGATCAAACATCGATCCAACAGCGTGGGAAGACAAGAAATCCAAAGGAGAATCAAGATTTCCAGCACAAGTTAGAATTCGAGCCAAGGAAAGGTGCAAACCACTAGAAGAGGATGCTTTTAGGCCAATACTCTACCACTATGATGGCCCAAAGTTTCGTTTACAACTCTCTGTGCCTGAGGTGCTTGCATTGTTAGACTTATTCAAAGAAGATCACCGGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

313

Amino Acids

36.39

Weight (kDa)

6.46

Isoelectric Point (pI)

58.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 180 - 307 1.6e-41 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 472
Acc36I ACCTGC 1 cut(s) 472
AciI CCGC 4 cut(s) 207, 311, 342, 478
AclWI GGATC 4 cut(s) 89, 588, 725, 727
AcsI RAATTY 2 cut(s) 320, 792
AfaI GTAC 3 cut(s) 78, 215, 617
AfiI CCNNNNNNNGG 1 cut(s) 807
AgsI TTSAA 3 cut(s) 286, 319, 922
AjnI CCWGG 1 cut(s) 643
AluBI AGCT 3 cut(s) 36, 122, 685
AluI AGCT 3 cut(s) 36, 122, 685
AlwI GGATC 4 cut(s) 89, 588, 725, 727
Ama87I CYCGRG 1 cut(s) 114
AoxI GGCC 3 cut(s) 507, 839, 862
ApeKI GCWGC 8 cut(s) 152, 358, 391, 400, 403, 475, 499, 517
ApoI RAATTY 2 cut(s) 320, 792
AspS9I GGNCC 1 cut(s) 863
AsuHPI GGTGA 1 cut(s) 923
AvaI CYCGRG 1 cut(s) 114
AxyI CCTNAGG 2 cut(s) 504, 894
BbsI GAAGAC 1 cut(s) 753
BbvI GCAGC 8 cut(s) 164, 370, 403, 412, 415, 486, 487, 504
BccI CCATC 1 cut(s) 854
BciT130I CCWGG 1 cut(s) 645
BclI TGATCA 2 cut(s) 124, 130
BfaI CTAG 2 cut(s) 522, 821
BfuAI ACCTGC 1 cut(s) 472
BmcAI AGTACT 1 cut(s) 215
Bme1390I CCNGG 1 cut(s) 645
BmeT110I CYCGRG 1 cut(s) 114
BmgT120I GGNCC 1 cut(s) 863
BmrFI CCNGG 1 cut(s) 645
BmsI GCATC 2 cut(s) 177, 820
BpiI GAAGAC 1 cut(s) 753
Bsa29I ATCGAT 1 cut(s) 729
BsaBI GATNNNNATC 3 cut(s) 129, 135, 228
BsaJI CCNNGG 3 cut(s) 643, 688, 801
Bsc4I CCNNNNNNNGG 1 cut(s) 807
Bse118I RCCGGY 1 cut(s) 933
Bse1I ACTGG 1 cut(s) 98
Bse21I CCTNAGG 2 cut(s) 504, 894
Bse8I GATNNNNATC 3 cut(s) 129, 135, 228
BseBI CCWGG 1 cut(s) 645
BseCI ATCGAT 1 cut(s) 729
BseDI CCNNGG 3 cut(s) 643, 688, 801
BseGI GGATG 1 cut(s) 835
BseJI GATNNNNATC 3 cut(s) 129, 135, 228
BseLI CCNNNNNNNGG 1 cut(s) 807
BseMII CTCAG 3 cut(s) 231, 518, 885
BseNI ACTGG 1 cut(s) 98
BseXI GCAGC 8 cut(s) 164, 370, 403, 412, 415, 486, 487, 504
BsgI GTGCAG 3 cut(s) 368, 377, 410
BshFI GGCC 3 cut(s) 509, 841, 864
BshVI ATCGAT 1 cut(s) 729
BsiHKCI CYCGRG 1 cut(s) 114
BsiSI CCGG 1 cut(s) 934
BslFI GGGAC 1 cut(s) 358
BslI CCNNNNNNNGG 1 cut(s) 807
BsmFI GGGAC 1 cut(s) 358
BsnI GGCC 3 cut(s) 509, 841, 864
BsoBI CYCGRG 1 cut(s) 114
Bsp143I GATC 8 cut(s) 94, 124, 130, 229, 580, 719, 730, 928
Bsp19I CCATGG 1 cut(s) 688
BspACI CCGC 4 cut(s) 207, 311, 342, 478
BspANI GGCC 3 cut(s) 509, 841, 864
BspCNI CTCAG 3 cut(s) 230, 517, 886
BspDI ATCGAT 1 cut(s) 729
BspHI TCATGA 3 cut(s) 88, 127, 445
BspMI ACCTGC 1 cut(s) 472
BspPI GGATC 4 cut(s) 89, 588, 725, 727
BsrFI RCCGGY 1 cut(s) 933
BsrI ACTGG 1 cut(s) 98
BssAI RCCGGY 1 cut(s) 933
BssECI CCNNGG 3 cut(s) 643, 688, 801
BssMI GATC 8 cut(s) 94, 124, 130, 229, 580, 719, 730, 928
BssT1I CCWWGG 2 cut(s) 688, 801
Bst2UI CCWGG 1 cut(s) 645
Bst4CI ACNGT 2 cut(s) 20, 590
Bst6I CTCTTC 2 cut(s) 602, 819
BstAPI GCANNNNNTGC 1 cut(s) 355
BstC8I GCNNGC 3 cut(s) 347, 351, 903
BstDEI CTNAG 3 cut(s) 217, 504, 894
BstDSI CCRYGG 1 cut(s) 688
BstF5I GGATG 1 cut(s) 835
BstKTI GATC 8 cut(s) 97, 127, 133, 232, 583, 722, 733, 931
BstMBI GATC 8 cut(s) 94, 124, 130, 229, 580, 719, 730, 928
BstMWI GCNNNNNNNGC 6 cut(s) 355, 397, 400, 487, 496, 898
BstNI CCWGG 1 cut(s) 645
BstSCI CCNGG 1 cut(s) 643
BstV1I GCAGC 8 cut(s) 164, 370, 403, 412, 415, 486, 487, 504
BstV2I GAAGAC 1 cut(s) 753
BstX2I RGATCY 1 cut(s) 580
BstXI CCANNNNNNTGG 3 cut(s) 689, 741, 860
BstYI RGATCY 1 cut(s) 580
Bsu15I ATCGAT 1 cut(s) 729
Bsu36I CCTNAGG 2 cut(s) 504, 894
BsuRI GGCC 3 cut(s) 509, 841, 864
BsuTUI ATCGAT 1 cut(s) 729
BtgI CCRYGG 1 cut(s) 688
BtsCI GGATG 1 cut(s) 835
BtsIMutI CAGTG 2 cut(s) 105, 167
BveI ACCTGC 1 cut(s) 472
Cac8I GCNNGC 3 cut(s) 347, 351, 903
CciI TCATGA 3 cut(s) 88, 127, 445
Cfr10I RCCGGY 1 cut(s) 933
Cfr13I GGNCC 1 cut(s) 863
ClaI ATCGAT 1 cut(s) 729
CseI GACGC 1 cut(s) 505
Csp6I GTAC 3 cut(s) 77, 214, 616
CviAII CATG 5 cut(s) 89, 128, 446, 571, 689
CviQI GTAC 3 cut(s) 77, 214, 616
DdeI CTNAG 3 cut(s) 217, 504, 894
DpnI GATC 8 cut(s) 96, 126, 132, 231, 582, 721, 732, 930
DpnII GATC 8 cut(s) 94, 124, 130, 229, 580, 719, 730, 928
Eam1104I CTCTTC 2 cut(s) 602, 819
EarI CTCTTC 2 cut(s) 602, 819
Eco130I CCWWGG 2 cut(s) 688, 801
Eco81I CCTNAGG 2 cut(s) 504, 894
Eco88I CYCGRG 1 cut(s) 114
EcoRI GAATTC 1 cut(s) 792
EcoRII CCWGG 1 cut(s) 643
EcoT14I CCWWGG 2 cut(s) 688, 801
ErhI CCWWGG 2 cut(s) 688, 801
FaeI CATG 5 cut(s) 92, 131, 449, 574, 692
FaqI GGGAC 1 cut(s) 358
FatI CATG 5 cut(s) 88, 127, 445, 570, 688
FbaI TGATCA 2 cut(s) 124, 130
FokI GGATG 1 cut(s) 842
FspBI CTAG 2 cut(s) 522, 821
HaeIII GGCC 3 cut(s) 509, 841, 864
HapII CCGG 1 cut(s) 934
HgaI GACGC 1 cut(s) 505
Hin1II CATG 5 cut(s) 92, 131, 449, 574, 692
HincII GTYRAC 1 cut(s) 593
HindII GTYRAC 1 cut(s) 593
HinfI GANTC 3 cut(s) 603, 623, 767
HpaII CCGG 1 cut(s) 934
HphI GGTGA 1 cut(s) 923
Hpy166II GTNNAC 2 cut(s) 593, 878
Hpy188I TCNGA 2 cut(s) 220, 602
Hpy188III TCNNGA 5 cut(s) 89, 114, 128, 446, 771
Hpy8I GTNNAC 2 cut(s) 593, 878
HpyAV CCTTC 2 cut(s) 235, 386
HpyCH4III ACNGT 2 cut(s) 20, 590
HpyCH4V TGCA 8 cut(s) 190, 349, 358, 391, 558, 574, 813, 905
HpyF10VI GCNNNNNNNGC 6 cut(s) 355, 397, 400, 487, 496, 898
HpyF3I CTNAG 3 cut(s) 217, 504, 894
Hsp92II CATG 5 cut(s) 92, 131, 449, 574, 692
Ksp22I TGATCA 2 cut(s) 124, 130
Kzo9I GATC 8 cut(s) 94, 124, 130, 229, 580, 719, 730, 928
LmnI GCTCC 1 cut(s) 690
Lsp1109I GCAGC 8 cut(s) 164, 370, 403, 412, 415, 486, 487, 504
LweI GCATC 2 cut(s) 177, 820
MaeI CTAG 2 cut(s) 522, 821
MaeIII GTNAC 2 cut(s) 55, 542
MalI GATC 8 cut(s) 96, 126, 132, 231, 582, 721, 732, 930
MboI GATC 8 cut(s) 94, 124, 130, 229, 580, 719, 730, 928
MboII GAAGA 6 cut(s) 239, 298, 589, 758, 836, 938
MfeI CAATTG 1 cut(s) 182
MflI RGATCY 1 cut(s) 580
MluCI AATT 7 cut(s) 13, 182, 280, 320, 335, 636, 792
MlyI GAGTC 2 cut(s) 597, 617
MmeI TCCRAC 1 cut(s) 758
MnlI CCTC 8 cut(s) 121, 513, 533, 571, 618, 710, 820, 889
MslI CAYNNNNRTG 3 cut(s) 384, 450, 858
MspA1I CMGCKG 1 cut(s) 478
MspI CCGG 1 cut(s) 934
MspR9I CCNGG 1 cut(s) 645
MunI CAATTG 1 cut(s) 182
MvaI CCWGG 1 cut(s) 645
MwoI GCNNNNNNNGC 6 cut(s) 355, 397, 400, 487, 496, 898
NcoI CCATGG 1 cut(s) 688
NdeII GATC 8 cut(s) 94, 124, 130, 229, 580, 719, 730, 928
NlaIII CATG 5 cut(s) 92, 131, 449, 574, 692
PagI TCATGA 3 cut(s) 88, 127, 445
PaqCI CACCTGC 1 cut(s) 472
PfeI GAWTC 1 cut(s) 767
PflFI GACNNNGTC 1 cut(s) 626
PleI GAGTC 2 cut(s) 597, 617
PpsI GAGTC 2 cut(s) 597, 617
Psp6I CCWGG 1 cut(s) 643
PspGI CCWGG 1 cut(s) 643
PspPI GGNCC 1 cut(s) 863
PsuI RGATCY 1 cut(s) 580
PsyI GACNNNGTC 1 cut(s) 626
RsaI GTAC 3 cut(s) 78, 215, 617
RsaNI GTAC 3 cut(s) 77, 214, 616
RseI CAYNNNNRTG 3 cut(s) 384, 450, 858
Sau3AI GATC 8 cut(s) 94, 124, 130, 229, 580, 719, 730, 928
Sau96I GGNCC 1 cut(s) 863
ScaI AGTACT 1 cut(s) 215
SchI GAGTC 2 cut(s) 597, 617
ScrFI CCNGG 1 cut(s) 645
SetI ASST 7 cut(s) 38, 124, 486, 544, 687, 812, 900
SfaNI GCATC 2 cut(s) 177, 820
SmiMI CAYNNNNRTG 3 cut(s) 384, 450, 858
Sse9I AATT 7 cut(s) 13, 182, 280, 320, 335, 636, 792
SsiI CCGC 4 cut(s) 207, 311, 342, 478
SspMI CTAG 2 cut(s) 522, 821
StyD4I CCNGG 1 cut(s) 643
StyI CCWWGG 2 cut(s) 688, 801
TaaI ACNGT 2 cut(s) 20, 590
TaqI TCGA 2 cut(s) 729, 796
TasI AATT 7 cut(s) 13, 182, 280, 320, 335, 636, 792
TatI WGTACW 3 cut(s) 76, 213, 615
TauI GCSGC 4 cut(s) 209, 314, 345, 481
TfiI GAWTC 1 cut(s) 767
TscAI CASTG 2 cut(s) 105, 174
TseI GCWGC 8 cut(s) 152, 358, 391, 400, 403, 475, 499, 517
TspDTI ATGAA 4 cut(s) 240, 347, 368, 543
TspRI CASTG 2 cut(s) 105, 174
Tth111I GACNNNGTC 1 cut(s) 626
XapI RAATTY 2 cut(s) 320, 792
XcmI CCANNNNNNNNNTGG 1 cut(s) 686
XspI CTAG 2 cut(s) 522, 821
ZrmI AGTACT 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.