Rh6DG002000

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002574
Physical Location & Seq
Reverse (-)
329871 .. 332001
2131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG002000.1

Sequence Viewer

Length: 513 bp
ATGATGAGCAGTAGTCGTGACAATGATTATTGTTATTACAATAAGCAGCAGCAGGTGTGGCATAAGACGCTGCCTCAGGCTGAAATGCTGCCTAGAAATGAAGTCTTGGGAGGTTACATTTTTGTATGCAACAACGAAACCATGCAAGAGGATCTCCACCGTCAACTCTTCGGACTCCCTCAAAAGTACAAGGACTCTGTAAGGGCAATTACCCCAGGCTTACCCCTTTTTCTTTACAACTACACAGCCCACCAGCTCCATGGAGTTTTTCAGGCTGTGAGTTTTGGAGGATCAAATATCGATCCAACAGCGTGGGAAGACAAGAAATCCAAAGGAGAATCAAGATTTCCAGCACAAGTTAGAATTCGAGCCAAGGAAAGGTGCAAACCACTAGAAGAGGATGCTTTTAGGCCAATACTCTACCACTATGACGGCCCAAAGTTTCGTTTACAACTCTCTGTGCCTGAGGTGCTTGCATTGTTAGACTTATTCAAAGAAGATCACCGGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

170

Amino Acids

19.87

Weight (kDa)

7.74

Isoelectric Point (pI)

54.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 37 - 164 4.4e-42 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 43
Acc36I ACCTGC 1 cut(s) 43
AclWI GGATC 3 cut(s) 159, 296, 298
AcsI RAATTY 1 cut(s) 363
AfaI GTAC 1 cut(s) 188
AfiI CCNNNNNNNGG 1 cut(s) 378
AgsI TTSAA 1 cut(s) 493
AjnI CCWGG 1 cut(s) 214
AluBI AGCT 1 cut(s) 256
AluI AGCT 1 cut(s) 256
AlwI GGATC 3 cut(s) 159, 296, 298
AoxI GGCC 2 cut(s) 410, 433
ApeKI GCWGC 4 cut(s) 46, 49, 70, 88
ApoI RAATTY 1 cut(s) 363
AspS9I GGNCC 1 cut(s) 434
AsuHPI GGTGA 1 cut(s) 494
AxyI CCTNAGG 2 cut(s) 75, 465
BbsI GAAGAC 1 cut(s) 324
BbvI GCAGC 4 cut(s) 57, 58, 61, 75
BceAI ACGGC 1 cut(s) 448
BciT130I CCWGG 1 cut(s) 216
BfaI CTAG 2 cut(s) 93, 392
BfuAI ACCTGC 1 cut(s) 43
BisI GCNGC 4 cut(s) 47, 50, 71, 89
BlsI GCNGC 4 cut(s) 48, 51, 72, 90
Bme1390I CCNGG 1 cut(s) 216
BmgT120I GGNCC 1 cut(s) 434
BmrFI CCNGG 1 cut(s) 216
BmsI GCATC 1 cut(s) 391
BpiI GAAGAC 1 cut(s) 324
Bsa29I ATCGAT 1 cut(s) 300
BsaJI CCNNGG 3 cut(s) 214, 259, 372
Bsc4I CCNNNNNNNGG 1 cut(s) 378
Bse118I RCCGGY 1 cut(s) 504
Bse21I CCTNAGG 2 cut(s) 75, 465
BseBI CCWGG 1 cut(s) 216
BseCI ATCGAT 1 cut(s) 300
BseDI CCNNGG 3 cut(s) 214, 259, 372
BseGI GGATG 1 cut(s) 406
BseLI CCNNNNNNNGG 1 cut(s) 378
BseMII CTCAG 2 cut(s) 89, 456
BseXI GCAGC 4 cut(s) 57, 58, 61, 75
BshFI GGCC 2 cut(s) 412, 435
BshVI ATCGAT 1 cut(s) 300
BsiSI CCGG 1 cut(s) 505
BslI CCNNNNNNNGG 1 cut(s) 378
BsnI GGCC 2 cut(s) 412, 435
Bsp143I GATC 4 cut(s) 151, 290, 301, 499
Bsp19I CCATGG 1 cut(s) 259
BspANI GGCC 2 cut(s) 412, 435
BspCNI CTCAG 2 cut(s) 88, 457
BspDI ATCGAT 1 cut(s) 300
BspMI ACCTGC 1 cut(s) 43
BspPI GGATC 3 cut(s) 159, 296, 298
BsrFI RCCGGY 1 cut(s) 504
BssAI RCCGGY 1 cut(s) 504
BssECI CCNNGG 3 cut(s) 214, 259, 372
BssMI GATC 4 cut(s) 151, 290, 301, 499
BssT1I CCWWGG 2 cut(s) 259, 372
Bst2UI CCWGG 1 cut(s) 216
Bst4CI ACNGT 1 cut(s) 161
Bst6I CTCTTC 2 cut(s) 173, 390
BstC8I GCNNGC 1 cut(s) 474
BstDEI CTNAG 2 cut(s) 75, 465
BstDSI CCRYGG 1 cut(s) 259
BstF5I GGATG 1 cut(s) 406
BstKTI GATC 4 cut(s) 154, 293, 304, 502
BstMBI GATC 4 cut(s) 151, 290, 301, 499
BstMWI GCNNNNNNNGC 3 cut(s) 58, 67, 469
BstNI CCWGG 1 cut(s) 216
BstSCI CCNGG 1 cut(s) 214
BstV1I GCAGC 4 cut(s) 57, 58, 61, 75
BstV2I GAAGAC 1 cut(s) 324
BstX2I RGATCY 1 cut(s) 151
BstXI CCANNNNNNTGG 2 cut(s) 260, 312
BstYI RGATCY 1 cut(s) 151
Bsu15I ATCGAT 1 cut(s) 300
Bsu36I CCTNAGG 2 cut(s) 75, 465
BsuRI GGCC 2 cut(s) 412, 435
BsuTUI ATCGAT 1 cut(s) 300
BtgI CCRYGG 1 cut(s) 259
BtsCI GGATG 1 cut(s) 406
BveI ACCTGC 1 cut(s) 43
Cac8I GCNNGC 1 cut(s) 474
Cfr10I RCCGGY 1 cut(s) 504
Cfr13I GGNCC 1 cut(s) 434
ClaI ATCGAT 1 cut(s) 300
CseI GACGC 1 cut(s) 76
Csp6I GTAC 1 cut(s) 187
CviAII CATG 2 cut(s) 142, 260
CviJI RGCY 9 cut(s) 80, 219, 248, 256, 275, 371, 412, 435, 508
CviKI_1 RGCY 9 cut(s) 80, 219, 248, 256, 275, 371, 412, 435, 508
CviQI GTAC 1 cut(s) 187
DdeI CTNAG 2 cut(s) 75, 465
DpnI GATC 4 cut(s) 153, 292, 303, 501
DpnII GATC 4 cut(s) 151, 290, 301, 499
Eam1104I CTCTTC 2 cut(s) 173, 390
EarI CTCTTC 2 cut(s) 173, 390
Eco130I CCWWGG 2 cut(s) 259, 372
Eco81I CCTNAGG 2 cut(s) 75, 465
EcoRI GAATTC 1 cut(s) 363
EcoRII CCWGG 1 cut(s) 214
EcoT14I CCWWGG 2 cut(s) 259, 372
ErhI CCWWGG 2 cut(s) 259, 372
FaeI CATG 2 cut(s) 145, 263
FaiI YATR 5 cut(s) 63, 127, 143, 261, 429
FatI CATG 2 cut(s) 141, 259
Fnu4HI GCNGC 4 cut(s) 47, 50, 71, 89
FokI GGATG 1 cut(s) 413
Fsp4HI GCNGC 4 cut(s) 47, 50, 71, 89
FspBI CTAG 2 cut(s) 93, 392
GluI GCNGC 4 cut(s) 47, 50, 71, 89
HaeIII GGCC 2 cut(s) 412, 435
HapII CCGG 1 cut(s) 505
HgaI GACGC 1 cut(s) 76
Hin1II CATG 2 cut(s) 145, 263
HincII GTYRAC 1 cut(s) 164
HindII GTYRAC 1 cut(s) 164
HinfI GANTC 3 cut(s) 174, 194, 338
HpaII CCGG 1 cut(s) 505
HphI GGTGA 1 cut(s) 494
Hpy166II GTNNAC 2 cut(s) 164, 449
Hpy188I TCNGA 1 cut(s) 173
Hpy188III TCNNGA 2 cut(s) 17, 342
Hpy8I GTNNAC 2 cut(s) 164, 449
HpyCH4III ACNGT 1 cut(s) 161
HpyCH4V TGCA 4 cut(s) 129, 145, 384, 476
HpyF10VI GCNNNNNNNGC 3 cut(s) 58, 67, 469
HpyF3I CTNAG 2 cut(s) 75, 465
Hsp92II CATG 2 cut(s) 145, 263
Kzo9I GATC 4 cut(s) 151, 290, 301, 499
LmnI GCTCC 1 cut(s) 261
LpnPI CCDG 8 cut(s) 38, 62, 201, 228, 257, 266, 363, 477
Lsp1109I GCAGC 4 cut(s) 57, 58, 61, 75
LweI GCATC 1 cut(s) 391
MaeI CTAG 2 cut(s) 93, 392
MaeIII GTNAC 2 cut(s) 17, 113
MalI GATC 4 cut(s) 153, 292, 303, 501
MboI GATC 4 cut(s) 151, 290, 301, 499
MboII GAAGA 4 cut(s) 160, 329, 407, 509
MflI RGATCY 1 cut(s) 151
MluCI AATT 2 cut(s) 207, 363
MlyI GAGTC 2 cut(s) 168, 188
MmeI TCCRAC 1 cut(s) 329
MnlI CCTC 7 cut(s) 84, 104, 142, 189, 281, 391, 460
MspI CCGG 1 cut(s) 505
MspR9I CCNGG 1 cut(s) 216
MvaI CCWGG 1 cut(s) 216
MwoI GCNNNNNNNGC 3 cut(s) 58, 67, 469
NcoI CCATGG 1 cut(s) 259
NdeII GATC 4 cut(s) 151, 290, 301, 499
NlaIII CATG 2 cut(s) 145, 263
NmuCI GTSAC 1 cut(s) 17
PaqCI CACCTGC 1 cut(s) 43
PfeI GAWTC 1 cut(s) 338
PkrI GCNGC 4 cut(s) 48, 51, 72, 90
PleI GAGTC 2 cut(s) 168, 188
PpsI GAGTC 2 cut(s) 168, 188
Psp6I CCWGG 1 cut(s) 214
PspGI CCWGG 1 cut(s) 214
PspPI GGNCC 1 cut(s) 434
PsuI RGATCY 1 cut(s) 151
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
SatI GCNGC 4 cut(s) 47, 50, 71, 89
Sau3AI GATC 4 cut(s) 151, 290, 301, 499
Sau96I GGNCC 1 cut(s) 434
SchI GAGTC 2 cut(s) 168, 188
ScrFI CCNGG 1 cut(s) 216
SetI ASST 5 cut(s) 57, 115, 258, 383, 471
SfaNI GCATC 1 cut(s) 391
Sse9I AATT 2 cut(s) 207, 363
SspMI CTAG 2 cut(s) 93, 392
StyD4I CCNGG 1 cut(s) 214
StyI CCWWGG 2 cut(s) 259, 372
TaaI ACNGT 1 cut(s) 161
TaqI TCGA 2 cut(s) 300, 367
TasI AATT 2 cut(s) 207, 363
TatI WGTACW 1 cut(s) 186
TfiI GAWTC 1 cut(s) 338
TseFI GTSAC 1 cut(s) 17
TseI GCWGC 4 cut(s) 46, 49, 70, 88
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 1 cut(s) 114
XapI RAATTY 1 cut(s) 363
XcmI CCANNNNNNNNNTGG 1 cut(s) 257
XspI CTAG 2 cut(s) 93, 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.