FvH4_7g03250

26S protease regulatory subunit 6B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002106
Physical Location & Seq
Forward (+)
3602784 .. 3607097
4314 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g03250.t1

Sequence Viewer

Length: 1227 bp
ATGGTGTCGGAGCCACCCGCTTCGTTCCCCTCCACAAGATCGGACCCCAAATCCAGCGAGCAGCCCCAATCCTCCGACGAGGACGACCTCTACAGCCGCCTGAAATCCCTGCAGCGCCAGCTGGAGTTCATCGACATCCAAGAGGAGTACGTGAAAGACGAGCAGAAGAATCTGAAGCGGGAGCTCCTCCGCGCCCAGGAGGAGGTGAAGAGGATCCAGTCGGTGCCTCTGGTGATCGGTCAATTCATGGAGATGGTGGATCAGAACAACGGCATCGTCGGGTCCACCACGGGATCCAATTACTACGTCAGGATTCTCAGTACTATTAATCGAGAGCATCTCAAGCCCTCCGCCTCCGTCGCCTTGCACCGCCACTCCAACGCCCTCGTCGACGTTCTGCCGCCCGAGGCCGACTCCAGTATTTCGCTTCTCAGCCAGTCCGAGAAGCCTGACGTCACCTATAACGATATCGGTGGTTGTGACATTCAAAAGCAAGAAATCCGTGAAGCAGTGGAACTTCCACTTACTCACCATGACTTGTACAAACAAATTGGAATAGATCCTCCACGTGGTGTATTGTTGTATGGTCCGCCTGGAACTGGTAAAACCATGCTAGCTAAGGCTGTTGCCAATCACACGACTGCTGCCTTCATTAGAGTTGTTGGTTCAGAATTTGTTCAGAAGTATTTGGGTGAGGGTCCACGAATGGTCCGTGATGTTTTCCGTCTCGCCAAAGAGAATGCACCTGCCATCATCTTCATTGATGAGGTGGATGCTATTGCTACTGCAAGGTTTGATGCTCAAACTGGAGCTGATAGAGAAGTCCAACGTATTCTTATGGAGCTCCTCAATCAGATGGATGGGTTTGACCAGACTGTGAATGTTAAGGTCATAATGGCTACTAATCGTGCTGACACTTTGGATCCTGCACTTCTGCGTCCAGGAAGGCTTGATCGGAAAATTGAATTCCCTTTGCCTGACAGGCGGCAGAAAAGACTTGTTTTTCAAGTCTGCACTGCTAAAATGAACTTGAGTGATGAGGTAGACTTGGAAGATTACGTCTCACGACCAGACAAAATCAGCGCTGCTGAGATTACAGCTATATGTCAAGAAGCAGGAATGCATGCAGTTAGAAAGAATCGATATGTCATACTGCCAAAGGACTTTGAGAAGGGATACCAAACCAATGTGAAAAAGCCTGATACTGACTTTGAGTTTTATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005911 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0030054 GO:0030162 GO:0030163 GO:0030312 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055044 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

409

Amino Acids

46.03

Weight (kDa)

5.48

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_ATP_ID_OB_C PF16450 79 - 134 1.5e-11 Proteasomal ATPase OB C-terminal domain
AAA_5 PF07728 191 - 313 6.4e-08 AAA domain (dynein-related subfamily)
AAA PF00004 192 - 325 2e-45 ATPase family associated with various cellular activities (AAA)
AAA_2 PF07724 192 - 285 2.8e-06 AAA domain (Cdc48 subfamily)
AAA_lid_3 PF17862 348 - 391 6.6e-07 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1221
AarI CACCTGC 1 cut(s) 754
AatII GACGTC 1 cut(s) 456
Acc36I ACCTGC 1 cut(s) 754
AccB1I GGYRCC 1 cut(s) 223
AccI GTMKAC 2 cut(s) 390, 1044
AccII CGCG 1 cut(s) 192
AciI CCGC 9 cut(s) 18, 97, 178, 190, 351, 370, 401, 590, 985
AclWI GGATC 8 cut(s) 208, 221, 267, 288, 301, 554, 917, 930
AcsI RAATTY 2 cut(s) 671, 965
AcuI CTGAAG 1 cut(s) 194
AcvI CACGTG 1 cut(s) 569
AcyI GRCGYC 1 cut(s) 453
AdeI CACNNNGTG 1 cut(s) 572
AfaI GTAC 3 cut(s) 149, 322, 542
AfeI AGCGCT 1 cut(s) 1084
AfiI CCNNNNNNNGG 4 cut(s) 196, 202, 569, 599
AgsI TTSAA 3 cut(s) 488, 965, 1007
AjnI CCWGG 3 cut(s) 195, 592, 940
AluBI AGCT 6 cut(s) 121, 184, 617, 812, 844, 1100
AluI AGCT 6 cut(s) 121, 184, 617, 812, 844, 1100
Alw21I GWGCWC 2 cut(s) 186, 846
Alw26I GTCTC 2 cut(s) 731, 1066
AlwI GGATC 8 cut(s) 208, 221, 267, 288, 301, 554, 917, 930
Ama87I CYCGRG 1 cut(s) 404
Aor51HI AGCGCT 1 cut(s) 1084
AoxI GGCC 1 cut(s) 408
ApeKI GCWGC 4 cut(s) 61, 112, 644, 1085
ApoI RAATTY 2 cut(s) 671, 965
AseI ATTAAT 1 cut(s) 327
Asp700I GAANNNNTTC 1 cut(s) 675
AspLEI GCGC 3 cut(s) 117, 194, 1085
AspS9I GGNCC 5 cut(s) 43, 282, 587, 698, 709
AsuHPI GGTGA 5 cut(s) 217, 244, 448, 521, 704
AsuNHI GCTAGC 1 cut(s) 613
AvaI CYCGRG 1 cut(s) 404
AvaII GGWCC 5 cut(s) 43, 282, 587, 698, 709
BamHI GGATCC 3 cut(s) 213, 293, 922
BanI GGYRCC 1 cut(s) 223
BanII GRGCYC 2 cut(s) 186, 846
BbrPI CACGTG 1 cut(s) 569
Bbv12I GWGCWC 2 cut(s) 186, 846
BbvI GCAGC 4 cut(s) 73, 124, 631, 1072
BccI CCATC 4 cut(s) 247, 758, 850, 854
BceAI ACGGC 1 cut(s) 286
BciT130I CCWGG 3 cut(s) 197, 594, 942
BciVI GTATCC 1 cut(s) 1169
BcoDI GTCTC 2 cut(s) 731, 1066
BfaI CTAG 1 cut(s) 614
BfmI CTRYAG 2 cut(s) 91, 110
BfoI RGCGCY 2 cut(s) 118, 1086
BfuAI ACCTGC 1 cut(s) 754
BfuI GTATCC 1 cut(s) 1169
BglI GCCNNNNNGGC 1 cut(s) 982
BisI GCNGC 7 cut(s) 62, 97, 113, 401, 645, 986, 1086
BlsI GCNGC 7 cut(s) 63, 98, 114, 402, 646, 987, 1087
BmcAI AGTACT 1 cut(s) 322
Bme1390I CCNGG 3 cut(s) 197, 594, 942
Bme18I GGWCC 5 cut(s) 43, 282, 587, 698, 709
BmeT110I CYCGRG 1 cut(s) 404
BmgT120I GGNCC 5 cut(s) 43, 282, 587, 698, 709
BmiI GGNNCC 8 cut(s) 12, 45, 215, 225, 283, 295, 699, 924
BmrFI CCNGG 3 cut(s) 197, 594, 942
BmsI GCATC 4 cut(s) 282, 346, 763, 787
BmtI GCTAGC 1 cut(s) 617
BplI GAGNNNNNCTC 4 cut(s) 324, 356, 398, 430
BpmI CTGGAG 3 cut(s) 143, 400, 828
Bpu10I CCTNAGC 1 cut(s) 618
BpuEI CTTGAG 2 cut(s) 326, 1051
Bsa29I ATCGAT 1 cut(s) 1141
BsaAI YACGTR 2 cut(s) 151, 569
BsaHI GRCGYC 1 cut(s) 453
BsaJI CCNNGG 3 cut(s) 195, 288, 405
BsaXI ACNNNNNCTCC 2 cut(s) 359, 389
Bsc4I CCNNNNNNNGG 4 cut(s) 196, 202, 569, 599
Bse1I ACTGG 5 cut(s) 217, 417, 436, 604, 811
BseBI CCWGG 3 cut(s) 197, 594, 942
BseCI ATCGAT 1 cut(s) 1141
BseDI CCNNGG 3 cut(s) 195, 288, 405
BseGI GGATG 3 cut(s) 135, 778, 865
BseLI CCNNNNNNNGG 4 cut(s) 196, 202, 569, 599
BseMII CTCAG 3 cut(s) 331, 445, 1080
BseNI ACTGG 5 cut(s) 217, 417, 436, 604, 811
BseRI GAGGAG 4 cut(s) 158, 176, 215, 836
BseXI GCAGC 4 cut(s) 73, 124, 631, 1072
BsgI GTGCAG 2 cut(s) 912, 997
Bsh1236I CGCG 1 cut(s) 192
BshFI GGCC 1 cut(s) 410
BshNI GGYRCC 1 cut(s) 223
BshVI ATCGAT 1 cut(s) 1141
BsiHKAI GWGCWC 2 cut(s) 186, 846
BsiHKCI CYCGRG 1 cut(s) 404
BslI CCNNNNNNNGG 4 cut(s) 196, 202, 569, 599
BsmAI GTCTC 2 cut(s) 731, 1066
BsmBI CGTCTC 2 cut(s) 731, 1066
BsmI GAATGC 2 cut(s) 745, 1125
BsnI GGCC 1 cut(s) 410
BsoBI CYCGRG 1 cut(s) 404
Bsp1286I GDGCHC 2 cut(s) 186, 846
Bsp1407I TGTACA 1 cut(s) 540
Bsp143I GATC 8 cut(s) 38, 213, 234, 259, 293, 559, 922, 952
BspACI CCGC 9 cut(s) 18, 97, 178, 190, 351, 370, 401, 590, 985
BspANI GGCC 1 cut(s) 410
BspCNI CTCAG 3 cut(s) 330, 444, 1081
BspDI ATCGAT 1 cut(s) 1141
BspFNI CGCG 1 cut(s) 192
BspLI GGNNCC 8 cut(s) 12, 45, 215, 225, 283, 295, 699, 924
BspMAI CTGCAG 1 cut(s) 114
BspMI ACCTGC 1 cut(s) 754
BspOI GCTAGC 1 cut(s) 617
BspPI GGATC 8 cut(s) 208, 221, 267, 288, 301, 554, 917, 930
BspT107I GGYRCC 1 cut(s) 223
BsrGI TGTACA 1 cut(s) 540
BsrI ACTGG 5 cut(s) 217, 417, 436, 604, 811
BssECI CCNNGG 3 cut(s) 195, 288, 405
BssMI GATC 8 cut(s) 38, 213, 234, 259, 293, 559, 922, 952
BssNI GRCGYC 1 cut(s) 453
Bst2UI CCWGG 3 cut(s) 197, 594, 942
Bst4CI ACNGT 1 cut(s) 877
Bst6I CTCTTC 1 cut(s) 203
BstACI GRCGYC 1 cut(s) 453
BstAUI TGTACA 1 cut(s) 540
BstBAI YACGTR 2 cut(s) 151, 569
BstC8I GCNNGC 4 cut(s) 59, 119, 615, 1125
BstDEI CTNAG 4 cut(s) 317, 431, 618, 1089
BstDSI CCRYGG 1 cut(s) 288
BstF5I GGATG 3 cut(s) 135, 778, 865
BstFNI CGCG 1 cut(s) 192
BstH2I RGCGCY 2 cut(s) 118, 1086
BstHHI GCGC 3 cut(s) 117, 194, 1085
BstKTI GATC 8 cut(s) 41, 216, 237, 262, 296, 562, 925, 955
BstMAI GTCTC 2 cut(s) 731, 1066
BstMBI GATC 8 cut(s) 38, 213, 234, 259, 293, 559, 922, 952
BstMWI GCNNNNNNNGC 4 cut(s) 118, 343, 359, 982
BstNI CCWGG 3 cut(s) 197, 594, 942
BstNSI RCATGY 1 cut(s) 1127
BstSCI CCNGG 3 cut(s) 195, 592, 940
BstSFI CTRYAG 2 cut(s) 91, 110
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 4 cut(s) 73, 124, 631, 1072
BstX2I RGATCY 4 cut(s) 213, 293, 559, 922
BstYI RGATCY 4 cut(s) 213, 293, 559, 922
Bsu15I ATCGAT 1 cut(s) 1141
BsuI GTATCC 1 cut(s) 1169
BsuRI GGCC 1 cut(s) 410
BsuTUI ATCGAT 1 cut(s) 1141
BtgI CCRYGG 1 cut(s) 288
BtsCI GGATG 3 cut(s) 135, 778, 865
BtsI GCAGTG 2 cut(s) 516, 1014
BtsIMutI CAGTG 2 cut(s) 516, 1014
BveI ACCTGC 1 cut(s) 754
Cac8I GCNNGC 4 cut(s) 59, 119, 615, 1125
CfoI GCGC 3 cut(s) 117, 194, 1085
Cfr13I GGNCC 5 cut(s) 43, 282, 587, 698, 709
ClaI ATCGAT 1 cut(s) 1141
CseI GACGC 1 cut(s) 926
Csp6I GTAC 3 cut(s) 148, 321, 541
CviAII CATG 4 cut(s) 247, 533, 610, 1124
CviQI GTAC 3 cut(s) 148, 321, 541
DdeI CTNAG 4 cut(s) 317, 431, 618, 1089
DpnI GATC 8 cut(s) 40, 215, 236, 261, 295, 561, 924, 954
DpnII GATC 8 cut(s) 38, 213, 234, 259, 293, 559, 922, 952
DraIII CACNNNGTG 1 cut(s) 572
Eam1104I CTCTTC 1 cut(s) 203
EarI CTCTTC 1 cut(s) 203
EciI GGCGGA 2 cut(s) 340, 579
Ecl136II GAGCTC 2 cut(s) 184, 844
Eco24I GRGCYC 2 cut(s) 186, 846
Eco32I GATATC 1 cut(s) 469
Eco47I GGWCC 5 cut(s) 43, 282, 587, 698, 709
Eco47III AGCGCT 1 cut(s) 1084
Eco53kI GAGCTC 2 cut(s) 184, 844
Eco57I CTGAAG 1 cut(s) 194
Eco72I CACGTG 1 cut(s) 569
Eco88I CYCGRG 1 cut(s) 404
EcoICRI GAGCTC 2 cut(s) 184, 844
EcoRI GAATTC 1 cut(s) 965
EcoRII CCWGG 3 cut(s) 195, 592, 940
EcoRV GATATC 1 cut(s) 469
EcoT22I ATGCAT 1 cut(s) 1125
EcoT38I GRGCYC 2 cut(s) 186, 846
Esp3I CGTCTC 2 cut(s) 731, 1066
FaeI CATG 4 cut(s) 250, 536, 613, 1127
FatI CATG 4 cut(s) 246, 532, 609, 1123
FauI CCCGC 2 cut(s) 25, 171
FblI GTMKAC 2 cut(s) 390, 1044
Fnu4HI GCNGC 7 cut(s) 62, 97, 113, 401, 645, 986, 1086
FokI GGATG 3 cut(s) 122, 785, 872
FriOI GRGCYC 2 cut(s) 186, 846
Fsp4HI GCNGC 7 cut(s) 62, 97, 113, 401, 645, 986, 1086
FspBI CTAG 1 cut(s) 614
GlaI GCGC 3 cut(s) 116, 193, 1084
GluI GCNGC 7 cut(s) 62, 97, 113, 401, 645, 986, 1086
GsuI CTGGAG 3 cut(s) 143, 400, 828
HaeII RGCGCY 2 cut(s) 118, 1086
HaeIII GGCC 1 cut(s) 410
HgaI GACGC 1 cut(s) 926
HhaI GCGC 3 cut(s) 117, 194, 1085
Hin1I GRCGYC 1 cut(s) 453
Hin1II CATG 4 cut(s) 250, 536, 613, 1127
Hin6I GCGC 3 cut(s) 115, 192, 1083
HinP1I GCGC 3 cut(s) 115, 192, 1083
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HinfI GANTC 4 cut(s) 169, 313, 413, 1138
HphI GGTGA 5 cut(s) 217, 244, 448, 521, 704
Hpy166II GTNNAC 4 cut(s) 285, 391, 701, 1045
Hpy188III TCNNGA 4 cut(s) 310, 332, 1065, 1109
Hpy8I GTNNAC 4 cut(s) 285, 391, 701, 1045
Hpy99I CGWCG 5 cut(s) 80, 281, 362, 392, 395
HpyAV CCTTC 3 cut(s) 658, 939, 1165
HpyCH4III ACNGT 1 cut(s) 877
HpyCH4IV ACGT 7 cut(s) 150, 306, 393, 453, 568, 829, 1059
HpyCH4V TGCA 8 cut(s) 112, 367, 743, 788, 929, 1014, 1123, 1127
HpyF10VI GCNNNNNNNGC 4 cut(s) 118, 343, 359, 982
HpyF3I CTNAG 4 cut(s) 317, 431, 618, 1089
HpySE526I ACGT 7 cut(s) 150, 306, 393, 453, 568, 829, 1059
Hsp92I GRCGYC 1 cut(s) 453
Hsp92II CATG 4 cut(s) 250, 536, 613, 1127
HspAI GCGC 3 cut(s) 115, 192, 1083
Kzo9I GATC 8 cut(s) 38, 213, 234, 259, 293, 559, 922, 952
LmnI GCTCC 6 cut(s) 10, 181, 189, 809, 841, 849
Lsp1109I GCAGC 4 cut(s) 73, 124, 631, 1072
LweI GCATC 4 cut(s) 282, 346, 763, 787
MaeI CTAG 1 cut(s) 614
MaeII ACGT 7 cut(s) 150, 306, 393, 453, 568, 829, 1059
MaeIII GTNAC 2 cut(s) 454, 479
MalI GATC 8 cut(s) 40, 215, 236, 261, 295, 561, 924, 954
MboI GATC 8 cut(s) 38, 213, 234, 259, 293, 559, 922, 952
MboII GAAGA 4 cut(s) 178, 220, 748, 1064
MflI RGATCY 4 cut(s) 213, 293, 559, 922
MhlI GDGCHC 2 cut(s) 186, 846
MluCI AATT 6 cut(s) 242, 298, 549, 671, 960, 965
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 3 cut(s) 99, 402, 850
Mph1103I ATGCAT 1 cut(s) 1125
MroXI GAANNNNTTC 1 cut(s) 675
MseI TTAA 2 cut(s) 327, 885
MslI CAYNNNNRTG 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 121
MspR9I CCNGG 3 cut(s) 197, 594, 942
Mva1269I GAATGC 2 cut(s) 745, 1125
MvaI CCWGG 3 cut(s) 197, 594, 942
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 4 cut(s) 118, 343, 359, 982
NdeII GATC 8 cut(s) 38, 213, 234, 259, 293, 559, 922, 952
NheI GCTAGC 1 cut(s) 613
NlaIII CATG 4 cut(s) 250, 536, 613, 1127
NlaIV GGNNCC 8 cut(s) 12, 45, 215, 225, 283, 295, 699, 924
NmuCI GTSAC 2 cut(s) 454, 479
NsiI ATGCAT 1 cut(s) 1125
NspI RCATGY 1 cut(s) 1127
PaeI GCATGC 1 cut(s) 1127
PaqCI CACCTGC 1 cut(s) 754
PcsI WCGNNNNNNNCGW 3 cut(s) 156, 387, 709
PctI GAATGC 2 cut(s) 745, 1125
PdmI GAANNNNTTC 1 cut(s) 675
PfeI GAWTC 3 cut(s) 169, 313, 1138
PfoI TCCNGGA 1 cut(s) 940
PkrI GCNGC 7 cut(s) 63, 98, 114, 402, 646, 987, 1087
PleI GAGTC 1 cut(s) 407
PmaCI CACGTG 1 cut(s) 569
PmlI CACGTG 1 cut(s) 569
PpsI GAGTC 1 cut(s) 407
Ppu21I YACGTR 2 cut(s) 151, 569
PshBI ATTAAT 1 cut(s) 327
PsiI TTATAA 1 cut(s) 1221
Psp124BI GAGCTC 2 cut(s) 186, 846
Psp6I CCWGG 3 cut(s) 195, 592, 940
PspCI CACGTG 1 cut(s) 569
PspGI CCWGG 3 cut(s) 195, 592, 940
PspN4I GGNNCC 8 cut(s) 12, 45, 215, 225, 283, 295, 699, 924
PspPI GGNCC 5 cut(s) 43, 282, 587, 698, 709
PstI CTGCAG 1 cut(s) 114
PsuI RGATCY 4 cut(s) 213, 293, 559, 922
PvuII CAGCTG 1 cut(s) 121
RsaI GTAC 3 cut(s) 149, 322, 542
RsaNI GTAC 3 cut(s) 148, 321, 541
RseI CAYNNNNRTG 1 cut(s) 251
SacI GAGCTC 2 cut(s) 186, 846
SalI GTCGAC 1 cut(s) 389
SaqAI TTAA 2 cut(s) 327, 885
SatI GCNGC 7 cut(s) 62, 97, 113, 401, 645, 986, 1086
Sau3AI GATC 8 cut(s) 38, 213, 234, 259, 293, 559, 922, 952
Sau96I GGNCC 5 cut(s) 43, 282, 587, 698, 709
ScaI AGTACT 1 cut(s) 322
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 3 cut(s) 197, 594, 942
SduI GDGCHC 2 cut(s) 186, 846
SfaNI GCATC 4 cut(s) 282, 346, 763, 787
SfcI CTRYAG 2 cut(s) 91, 110
SgrDI CGTCGACG 1 cut(s) 389
SinI GGWCC 5 cut(s) 43, 282, 587, 698, 709
SmiMI CAYNNNNRTG 1 cut(s) 251
SmlI CTYRAG 2 cut(s) 341, 1030
SmoI CTYRAG 2 cut(s) 341, 1030
SphI GCATGC 1 cut(s) 1127
Sse9I AATT 6 cut(s) 242, 298, 549, 671, 960, 965
SsiI CCGC 9 cut(s) 18, 97, 178, 190, 351, 370, 401, 590, 985
SspMI CTAG 1 cut(s) 614
SstI GAGCTC 2 cut(s) 186, 846
StyD4I CCNGG 3 cut(s) 195, 592, 940
TaaI ACNGT 1 cut(s) 877
TaiI ACGT 7 cut(s) 153, 309, 396, 456, 571, 832, 1062
TaqI TCGA 4 cut(s) 132, 331, 390, 1141
TaqII GACCGA 1 cut(s) 227
TasI AATT 6 cut(s) 242, 298, 549, 671, 960, 965
TatI WGTACW 2 cut(s) 320, 540
TauI GCSGC 3 cut(s) 99, 403, 988
TfiI GAWTC 3 cut(s) 169, 313, 1138
Tru1I TTAA 2 cut(s) 327, 885
Tru9I TTAA 2 cut(s) 327, 885
TscAI CASTG 2 cut(s) 516, 1021
TseFI GTSAC 2 cut(s) 454, 479
TseI GCWGC 4 cut(s) 61, 112, 644, 1085
Tsp45I GTSAC 2 cut(s) 454, 479
TspDTI ATGAA 5 cut(s) 118, 235, 640, 748, 1040
TspGWI ACGGA 4 cut(s) 346, 491, 701, 713
TspRI CASTG 2 cut(s) 516, 1021
VpaK11BI GGWCC 5 cut(s) 43, 282, 587, 698, 709
VspI ATTAAT 1 cut(s) 327
XapI RAATTY 2 cut(s) 671, 965
XceI RCATGY 1 cut(s) 1127
XmiI GTMKAC 2 cut(s) 390, 1044
XmnI GAANNNNTTC 1 cut(s) 675
XspI CTAG 1 cut(s) 614
ZraI GACGTC 1 cut(s) 454
ZrmI AGTACT 1 cut(s) 322
Zsp2I ATGCAT 1 cut(s) 1125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.