MD05G1031100.v1.1

26S protease regulatory subunit 6B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002106
Physical Location & Seq
Forward (+)
5031593 .. 5036574
4982 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1031100.v1.1.491

Sequence Viewer

Length: 1248 bp
ATGGTGTTGGATCCAAAACCTTTTCCTGAGCCACCAGCTTCAATCCCCTCCATAAGATTGGACCTTCAGGGCTACACCGACCAAAACCAGGCCTCCGATGAGGATGATCTCTACAGCCGCCTCAAGTCCTATCAGCGACAGCTTGAGTTCATCGACATCCAGGAGGAGTATGTCAAGGACGAGCAGAAGAATCTCAAGCGTGAGCTCCTCCGCGCCCAGGAAGAAGTCAAGAGGATCCAATCGGTGCCGCTCGTCATCGGGCAATTCATGGAGATGGTCGACCAGAACAACGGGATTGTGGGCTCCACCACTGGCTCCAATTACTATGTTAGGATTCTCAGCACCATTAATCGGGAGCTCCTCAAGCCTTCTGCTTCTGTTGCCTTGCACCGCCACTCCAATGCCCTTGTCGACGTTTTGCCTCCGGAGGCTGACTCAAGTATTTCCCTCCTCAGCCAGTCTGAGAAGCCCGACGTCACCTACTCTGATATTGGAGGATGTGACATTCAGAAGCAAGAAATCCGTGAAGCAGTAGAACTGCCACTTACCCATCACGAGTTGTACAAACAGATTGGAATAGATCCCCCACGTGGTGTATTGCTTTATGGTCCACCTGGAACTGGTAAAACCATGTTAGCAAAGGCCGTGGCTAATCATACAACTGCTGCCTTCATCAGAGTTGTTGGTTCAGAATTTGTTCAGAAGTATTTGGGAGAGGGTCCACGAATGGTTCGTGATGTTTTCCGTCTTGCCAAGGAGAATGCACCTGCCATCATCTTTATTGATGAGGTGGATGCTATTGCTACTGCGAGGTTTGATGCTCAAACTGGAGCTGACAGAGAAGTTCAGAGAATCCTTATGGAGCTCCTGAATCAGATGGATGGGTTTGACCAGACAGTGAATGTTAAGGTTATTATGGCTACTAACCGAGCAGACACCTTGGATCCTGCACTTCTGCGTCCTGGAAGGCTTGATCGGAAGATTGAGTTTCCTTTGCCTGATAGGAGGCAGAAAAGACTTGTTTTTCAGGTTTGCACCGCTAAAATGAACTTGAGTGATGAGGTAGACTTGGAAGATTATGTCTCTCGGCCAGACAAAATCAGTGCTGCTGAGATTTCAGCTATATGTCAAGAAGCAGGAATGCATGCAGTTCGGAAGAACCGATATGTCATACTCCCAAAGGACTTTGAAAAGGGTTACCAAACCAACGTGAAGAAGCCCGACACTGACTTTGAATTCTACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005911 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0030054 GO:0030162 GO:0030163 GO:0030312 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055044 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

416

Amino Acids

46.89

Weight (kDa)

5.33

Isoelectric Point (pI)

34.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_ATP_ID_OB_C PF16450 86 - 141 8.6e-12 Proteasomal ATPase OB C-terminal domain
AAA_5 PF07728 198 - 320 6.6e-08 AAA domain (dynein-related subfamily)
AAA PF00004 199 - 332 2e-45 ATPase family associated with various cellular activities (AAA)
AAA_2 PF07724 199 - 292 2.8e-06 AAA domain (Cdc48 subfamily)
AAA_lid_3 PF17862 355 - 398 5.9e-07 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 775
AatII GACGTC 1 cut(s) 477
Acc36I ACCTGC 1 cut(s) 775
AccB1I GGYRCC 1 cut(s) 244
AccBSI CCGCTC 1 cut(s) 250
AccI GTMKAC 3 cut(s) 279, 411, 1065
AccII CGCG 1 cut(s) 213
AccIII TCCGGA 1 cut(s) 424
AciI CCGC 5 cut(s) 118, 211, 248, 391, 1038
AclWI GGATC 7 cut(s) 5, 18, 229, 242, 575, 938, 951
AcoI YGGCCR 1 cut(s) 1088
AcsI RAATTY 2 cut(s) 692, 1235
AcuI CTGAAG 1 cut(s) 50
AcvI CACGTG 1 cut(s) 590
AcyI GRCGYC 1 cut(s) 474
AdeI CACNNNGTG 1 cut(s) 593
AfaI GTAC 1 cut(s) 563
AfiI CCNNNNNNNGG 5 cut(s) 88, 217, 351, 590, 620
AgsI TTSAA 3 cut(s) 42, 1190, 1235
AjnI CCWGG 5 cut(s) 87, 159, 216, 613, 961
AluBI AGCT 7 cut(s) 38, 142, 205, 358, 833, 865, 1121
AluI AGCT 7 cut(s) 38, 142, 205, 358, 833, 865, 1121
Alw21I GWGCWC 3 cut(s) 207, 360, 867
Alw26I GTCTC 1 cut(s) 1087
AlwI GGATC 7 cut(s) 5, 18, 229, 242, 575, 938, 951
Aor13HI TCCGGA 1 cut(s) 424
AoxI GGCC 3 cut(s) 90, 642, 1088
ApeKI GCWGC 2 cut(s) 665, 1106
ApoI RAATTY 2 cut(s) 692, 1235
ArsI GACNNNNNNTTYG 2 cut(s) 1214, 1246
AseI ATTAAT 1 cut(s) 348
Asp700I GAANNNNTTC 1 cut(s) 696
AspLEI GCGC 1 cut(s) 215
AspS9I GGNCC 3 cut(s) 61, 608, 719
AsuHPI GGTGA 1 cut(s) 469
AvaII GGWCC 3 cut(s) 61, 608, 719
BamHI GGATCC 3 cut(s) 10, 234, 943
BanI GGYRCC 1 cut(s) 244
BanII GRGCYC 4 cut(s) 207, 305, 360, 867
BauI CACGAG 1 cut(s) 554
BbrPI CACGTG 1 cut(s) 590
Bbv12I GWGCWC 3 cut(s) 207, 360, 867
BbvCI CCTCAGC 1 cut(s) 452
BbvI GCAGC 2 cut(s) 652, 1093
BccI CCATC 5 cut(s) 268, 558, 779, 871, 875
BceAI ACGGC 1 cut(s) 629
BcgI CGANNNNNNTGC 2 cut(s) 1133, 1167
BciT130I CCWGG 5 cut(s) 89, 161, 218, 615, 963
BcoDI GTCTC 1 cut(s) 1087
BfmI CTRYAG 1 cut(s) 112
BfuAI ACCTGC 1 cut(s) 775
BisI GCNGC 4 cut(s) 118, 248, 666, 1107
BlsI GCNGC 4 cut(s) 119, 249, 667, 1108
Bme1390I CCNGG 5 cut(s) 89, 161, 218, 615, 963
Bme18I GGWCC 3 cut(s) 61, 608, 719
BmgT120I GGNCC 3 cut(s) 61, 608, 719
BmiI GGNNCC 7 cut(s) 12, 236, 246, 304, 316, 720, 945
BmrFI CCNGG 5 cut(s) 89, 161, 218, 615, 963
BmsI GCATC 2 cut(s) 784, 808
BplI GAGNNNNNCTC 2 cut(s) 419, 451
BpmI CTGGAG 1 cut(s) 849
Bpu10I CCTNAGC 2 cut(s) 27, 452
BpuEI CTTGAG 6 cut(s) 107, 164, 179, 347, 421, 1072
BsaAI YACGTR 1 cut(s) 590
BsaHI GRCGYC 1 cut(s) 474
BsaJI CCNNGG 4 cut(s) 216, 645, 753, 939
BsaWI WCCGGW 1 cut(s) 424
BsaXI ACNNNNNCTCC 4 cut(s) 77, 107, 380, 410
Bsc4I CCNNNNNNNGG 5 cut(s) 88, 217, 351, 590, 620
Bse1I ACTGG 4 cut(s) 316, 457, 625, 832
BseAI TCCGGA 1 cut(s) 424
BseBI CCWGG 5 cut(s) 89, 161, 218, 615, 963
BseDI CCNNGG 4 cut(s) 216, 645, 753, 939
BseGI GGATG 5 cut(s) 109, 156, 503, 799, 886
BseLI CCNNNNNNNGG 5 cut(s) 88, 217, 351, 590, 620
BseMII CTCAG 5 cut(s) 18, 352, 453, 466, 1101
BseNI ACTGG 4 cut(s) 316, 457, 625, 832
BseRI GAGGAG 4 cut(s) 179, 197, 350, 440
BseXI GCAGC 2 cut(s) 652, 1093
BsgI GTGCAG 1 cut(s) 933
Bsh1236I CGCG 1 cut(s) 213
BshFI GGCC 3 cut(s) 92, 644, 1090
BshNI GGYRCC 1 cut(s) 244
BsiHKAI GWGCWC 3 cut(s) 207, 360, 867
BsiSI CCGG 1 cut(s) 425
BslI CCNNNNNNNGG 5 cut(s) 88, 217, 351, 590, 620
BsmAI GTCTC 1 cut(s) 1087
BsmI GAATGC 2 cut(s) 766, 1146
BsnI GGCC 3 cut(s) 92, 644, 1090
Bsp1286I GDGCHC 4 cut(s) 207, 305, 360, 867
Bsp13I TCCGGA 1 cut(s) 424
Bsp1407I TGTACA 1 cut(s) 561
Bsp143I GATC 6 cut(s) 10, 106, 234, 580, 943, 973
BspACI CCGC 5 cut(s) 118, 211, 248, 391, 1038
BspANI GGCC 3 cut(s) 92, 644, 1090
BspCNI CTCAG 5 cut(s) 19, 351, 454, 465, 1102
BspEI TCCGGA 1 cut(s) 424
BspFNI CGCG 1 cut(s) 213
BspLI GGNNCC 7 cut(s) 12, 236, 246, 304, 316, 720, 945
BspMI ACCTGC 1 cut(s) 775
BspPI GGATC 7 cut(s) 5, 18, 229, 242, 575, 938, 951
BspT107I GGYRCC 1 cut(s) 244
BsrBI CCGCTC 1 cut(s) 250
BsrGI TGTACA 1 cut(s) 561
BsrI ACTGG 4 cut(s) 316, 457, 625, 832
BssECI CCNNGG 4 cut(s) 216, 645, 753, 939
BssMI GATC 6 cut(s) 10, 106, 234, 580, 943, 973
BssNI GRCGYC 1 cut(s) 474
BssSI CACGAG 1 cut(s) 554
BssT1I CCWWGG 2 cut(s) 753, 939
Bst2BI CACGAG 1 cut(s) 554
Bst2UI CCWGG 5 cut(s) 89, 161, 218, 615, 963
Bst4CI ACNGT 1 cut(s) 898
BstACI GRCGYC 1 cut(s) 474
BstAUI TGTACA 1 cut(s) 561
BstBAI YACGTR 1 cut(s) 590
BstC8I GCNNGC 1 cut(s) 1146
BstDEI CTNAG 5 cut(s) 27, 338, 452, 462, 1110
BstDSI CCRYGG 1 cut(s) 645
BstEII GGTNACC 1 cut(s) 1196
BstF5I GGATG 5 cut(s) 109, 156, 503, 799, 886
BstFNI CGCG 1 cut(s) 213
BstHHI GCGC 1 cut(s) 215
BstKTI GATC 6 cut(s) 13, 109, 237, 583, 946, 976
BstMAI GTCTC 1 cut(s) 1087
BstMBI GATC 6 cut(s) 10, 106, 234, 580, 943, 973
BstMWI GCNNNNNNNGC 2 cut(s) 364, 380
BstNI CCWGG 5 cut(s) 89, 161, 218, 615, 963
BstNSI RCATGY 1 cut(s) 1148
BstPI GGTNACC 1 cut(s) 1196
BstSCI CCNGG 5 cut(s) 87, 159, 216, 613, 961
BstSFI CTRYAG 1 cut(s) 112
BstUI CGCG 1 cut(s) 213
BstV1I GCAGC 2 cut(s) 652, 1093
BstX2I RGATCY 4 cut(s) 10, 234, 580, 943
BstXI CCANNNNNNTGG 1 cut(s) 58
BstYI RGATCY 4 cut(s) 10, 234, 580, 943
BsuRI GGCC 3 cut(s) 92, 644, 1090
BtgI CCRYGG 1 cut(s) 645
BtsCI GGATG 5 cut(s) 109, 156, 503, 799, 886
BtsIMutI CAGTG 4 cut(s) 309, 903, 1108, 1224
BveI ACCTGC 1 cut(s) 775
Cac8I GCNNGC 1 cut(s) 1146
CfoI GCGC 1 cut(s) 215
Cfr13I GGNCC 3 cut(s) 61, 608, 719
CseI GACGC 1 cut(s) 947
Csp6I GTAC 1 cut(s) 562
CspCI CAANNNNNGTGG 2 cut(s) 627, 662
CviAII CATG 3 cut(s) 268, 631, 1145
CviQI GTAC 1 cut(s) 562
DdeI CTNAG 5 cut(s) 27, 338, 452, 462, 1110
DpnI GATC 6 cut(s) 12, 108, 236, 582, 945, 975
DpnII GATC 6 cut(s) 10, 106, 234, 580, 943, 973
DraIII CACNNNGTG 1 cut(s) 593
EaeI YGGCCR 1 cut(s) 1088
Ecl136II GAGCTC 3 cut(s) 205, 358, 865
Eco130I CCWWGG 2 cut(s) 753, 939
Eco147I AGGCCT 1 cut(s) 92
Eco24I GRGCYC 4 cut(s) 207, 305, 360, 867
Eco47I GGWCC 3 cut(s) 61, 608, 719
Eco53kI GAGCTC 3 cut(s) 205, 358, 865
Eco57I CTGAAG 1 cut(s) 50
Eco72I CACGTG 1 cut(s) 590
Eco91I GGTNACC 1 cut(s) 1196
EcoICRI GAGCTC 3 cut(s) 205, 358, 865
EcoO65I GGTNACC 1 cut(s) 1196
EcoRI GAATTC 1 cut(s) 1235
EcoRII CCWGG 5 cut(s) 87, 159, 216, 613, 961
EcoT14I CCWWGG 2 cut(s) 753, 939
EcoT22I ATGCAT 1 cut(s) 1146
EcoT38I GRGCYC 4 cut(s) 207, 305, 360, 867
ErhI CCWWGG 2 cut(s) 753, 939
FaeI CATG 3 cut(s) 271, 634, 1148
FatI CATG 3 cut(s) 267, 630, 1144
FblI GTMKAC 3 cut(s) 279, 411, 1065
Fnu4HI GCNGC 4 cut(s) 118, 248, 666, 1107
FokI GGATG 5 cut(s) 116, 143, 510, 806, 893
FriOI GRGCYC 4 cut(s) 207, 305, 360, 867
Fsp4HI GCNGC 4 cut(s) 118, 248, 666, 1107
GlaI GCGC 1 cut(s) 214
GluI GCNGC 4 cut(s) 118, 248, 666, 1107
GsuI CTGGAG 1 cut(s) 849
HaeIII GGCC 3 cut(s) 92, 644, 1090
HapII CCGG 1 cut(s) 425
HgaI GACGC 1 cut(s) 947
HhaI GCGC 1 cut(s) 215
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 3 cut(s) 271, 634, 1148
Hin6I GCGC 1 cut(s) 213
HinP1I GCGC 1 cut(s) 213
HincII GTYRAC 2 cut(s) 280, 412
HindII GTYRAC 2 cut(s) 280, 412
HinfI GANTC 5 cut(s) 190, 334, 434, 852, 871
HpaII CCGG 1 cut(s) 425
HphI GGTGA 1 cut(s) 469
Hpy166II GTNNAC 5 cut(s) 280, 412, 611, 722, 1066
Hpy188III TCNNGA 8 cut(s) 26, 229, 353, 425, 554, 734, 868, 1130
Hpy8I GTNNAC 5 cut(s) 280, 412, 611, 722, 1066
Hpy99I CGWCG 2 cut(s) 416, 476
HpyAV CCTTC 4 cut(s) 74, 378, 679, 960
HpyCH4III ACNGT 1 cut(s) 898
HpyCH4IV ACGT 4 cut(s) 414, 474, 589, 1209
HpyCH4V TGCA 6 cut(s) 388, 764, 950, 1035, 1144, 1148
HpyF10VI GCNNNNNNNGC 2 cut(s) 364, 380
HpyF3I CTNAG 5 cut(s) 27, 338, 452, 462, 1110
HpySE526I ACGT 4 cut(s) 414, 474, 589, 1209
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 3 cut(s) 271, 634, 1148
HspAI GCGC 1 cut(s) 213
Kpn2I TCCGGA 1 cut(s) 424
Kzo9I GATC 6 cut(s) 10, 106, 234, 580, 943, 973
LmnI GCTCC 8 cut(s) 210, 308, 320, 355, 363, 830, 862, 870
Lsp1109I GCAGC 2 cut(s) 652, 1093
LweI GCATC 2 cut(s) 784, 808
MaeII ACGT 4 cut(s) 414, 474, 589, 1209
MaeIII GTNAC 3 cut(s) 475, 500, 1196
MalI GATC 6 cut(s) 12, 108, 236, 582, 945, 975
MbiI CCGCTC 1 cut(s) 250
MboI GATC 6 cut(s) 10, 106, 234, 580, 943, 973
MboII GAAGA 6 cut(s) 199, 233, 991, 1085, 1168, 1225
MflI RGATCY 4 cut(s) 10, 234, 580, 943
MhlI GDGCHC 4 cut(s) 207, 305, 360, 867
MluCI AATT 4 cut(s) 263, 319, 692, 1235
MlyI GAGTC 1 cut(s) 428
Mph1103I ATGCAT 1 cut(s) 1146
MroI TCCGGA 1 cut(s) 424
MroXI GAANNNNTTC 1 cut(s) 696
MseI TTAA 2 cut(s) 348, 906
MslI CAYNNNNRTG 2 cut(s) 272, 399
MspI CCGG 1 cut(s) 425
MspR9I CCNGG 5 cut(s) 89, 161, 218, 615, 963
Mva1269I GAATGC 2 cut(s) 766, 1146
MvaI CCWGG 5 cut(s) 89, 161, 218, 615, 963
MvnI CGCG 1 cut(s) 213
MwoI GCNNNNNNNGC 2 cut(s) 364, 380
NdeII GATC 6 cut(s) 10, 106, 234, 580, 943, 973
NlaIII CATG 3 cut(s) 271, 634, 1148
NlaIV GGNNCC 7 cut(s) 12, 236, 246, 304, 316, 720, 945
NmeAIII GCCGAG 1 cut(s) 1066
NmuCI GTSAC 2 cut(s) 475, 500
NsiI ATGCAT 1 cut(s) 1146
NspI RCATGY 1 cut(s) 1148
PaeI GCATGC 1 cut(s) 1148
PaqCI CACCTGC 1 cut(s) 775
PceI AGGCCT 1 cut(s) 92
PcsI WCGNNNNNNNCGW 2 cut(s) 730, 1159
PctI GAATGC 2 cut(s) 766, 1146
PdmI GAANNNNTTC 1 cut(s) 696
PfeI GAWTC 4 cut(s) 190, 334, 852, 871
PfoI TCCNGGA 2 cut(s) 159, 961
PkrI GCNGC 4 cut(s) 119, 249, 667, 1108
PleI GAGTC 1 cut(s) 428
PmaCI CACGTG 1 cut(s) 590
PmlI CACGTG 1 cut(s) 590
PpsI GAGTC 1 cut(s) 428
Ppu21I YACGTR 1 cut(s) 590
PshBI ATTAAT 1 cut(s) 348
Psp124BI GAGCTC 3 cut(s) 207, 360, 867
Psp6I CCWGG 5 cut(s) 87, 159, 216, 613, 961
PspCI CACGTG 1 cut(s) 590
PspEI GGTNACC 1 cut(s) 1196
PspGI CCWGG 5 cut(s) 87, 159, 216, 613, 961
PspN4I GGNNCC 7 cut(s) 12, 236, 246, 304, 316, 720, 945
PspPI GGNCC 3 cut(s) 61, 608, 719
PsuI RGATCY 4 cut(s) 10, 234, 580, 943
RsaI GTAC 1 cut(s) 563
RsaNI GTAC 1 cut(s) 562
RseI CAYNNNNRTG 2 cut(s) 272, 399
SacI GAGCTC 3 cut(s) 207, 360, 867
SalI GTCGAC 2 cut(s) 278, 410
SaqAI TTAA 2 cut(s) 348, 906
SatI GCNGC 4 cut(s) 118, 248, 666, 1107
Sau3AI GATC 6 cut(s) 10, 106, 234, 580, 943, 973
Sau96I GGNCC 3 cut(s) 61, 608, 719
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 5 cut(s) 89, 161, 218, 615, 963
SduI GDGCHC 4 cut(s) 207, 305, 360, 867
SfaNI GCATC 2 cut(s) 784, 808
SfcI CTRYAG 1 cut(s) 112
SinI GGWCC 3 cut(s) 61, 608, 719
SmiMI CAYNNNNRTG 2 cut(s) 272, 399
SmlI CTYRAG 6 cut(s) 122, 143, 194, 362, 436, 1051
SmoI CTYRAG 6 cut(s) 122, 143, 194, 362, 436, 1051
SphI GCATGC 1 cut(s) 1148
Sse9I AATT 4 cut(s) 263, 319, 692, 1235
SseBI AGGCCT 1 cut(s) 92
SsiI CCGC 5 cut(s) 118, 211, 248, 391, 1038
SstI GAGCTC 3 cut(s) 207, 360, 867
StuI AGGCCT 1 cut(s) 92
StyD4I CCNGG 5 cut(s) 87, 159, 216, 613, 961
StyI CCWWGG 2 cut(s) 753, 939
TaaI ACNGT 1 cut(s) 898
TaiI ACGT 4 cut(s) 417, 477, 592, 1212
TaqI TCGA 3 cut(s) 153, 279, 411
TasI AATT 4 cut(s) 263, 319, 692, 1235
TatI WGTACW 1 cut(s) 561
TauI GCSGC 2 cut(s) 120, 250
TfiI GAWTC 4 cut(s) 190, 334, 852, 871
Tru1I TTAA 2 cut(s) 348, 906
Tru9I TTAA 2 cut(s) 348, 906
TscAI CASTG 4 cut(s) 316, 903, 1108, 1231
TseFI GTSAC 2 cut(s) 475, 500
TseI GCWGC 2 cut(s) 665, 1106
Tsp45I GTSAC 2 cut(s) 475, 500
TspDTI ATGAA 4 cut(s) 139, 256, 661, 1061
TspGWI ACGGA 2 cut(s) 512, 734
TspRI CASTG 4 cut(s) 316, 903, 1108, 1231
VpaK11BI GGWCC 3 cut(s) 61, 608, 719
VspI ATTAAT 1 cut(s) 348
XapI RAATTY 2 cut(s) 692, 1235
XceI RCATGY 1 cut(s) 1148
XmiI GTMKAC 3 cut(s) 279, 411, 1065
XmnI GAANNNNTTC 1 cut(s) 696
ZraI GACGTC 1 cut(s) 475
Zsp2I ATGCAT 1 cut(s) 1146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.