pycom03g03850

26S protease regulatory subunit 6B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0002106
Physical Location & Seq
Forward (+)
3047306 .. 3049811
2506 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g03850.3

Sequence Viewer

Length: 990 bp
ATGGCAGGCTCAGCCATGGTGTTGGATCCGAAGCCCTTGCCGGAACCACCGGCATCGATCACCTCCATAAGATCCGACCTCCAGGGCTACACCGAGCAGAGCCAGGCCTCCGACGAGGACGATCTCTACAGCCGCCTCAAATCCTACCAGCGGCAGCTCGAGTTCATCGACATCCAGGAGGAGTACGTCAAGGACGAGCTGAAGAATCTGAAGCGCGAGCTCCTCCGCGCCCAGGAGGAGGTCAAGAGGATCCAATCGGTGCCGCTCGTCATCGGGCAGTTCATGGAGATGGTCGACCAGAACAACGGGATTGTGGGCTCCACCACCGGCTCCAATTACTATGTTAGGATTCTCAGCACCATTAATCGGGAGCTCCTCAAGCCTTCCGCTTCCGTCGCCTTGCACCGCCACTCCAATGCCCTCGTCGACGTTCTGCCTCCGGAGGCCGACTCGAGTATTTCTCTCCTCAGCCAGTCTGAGAAGCCCGACGTCACCTACTCTGATATTGGAGGATGTGACATTCAGAAGCAAGAAATTCGGGAAGCGGTAGAACTGCCACTTACTCACCACGAGTTATACAAACAGATTGGAATAGATCCCCCACGTGGTGTATTACTCTATGGTCCACCTGGAACCGGTAAAACCATGTTAGCGAAGGCTGTTGCTAATCATACAACTGCTGCCTTCATCAGAGTTGTTGGTTCAGAATTTGTTCAGAAGTATTTGGGAGAGGGTCCACGAATGGTACGTGATGTGTTCCGTCTTGCCAAGGAGAATGCTCCTGCCATCATCTTTATTGATGAGGTGGATGCTATTGCTACTGCTAGGTTTGATGCTCAAACTGGAGCTGACAGAGAAGTTCAGCGAATCCTTATGGAGCTCCTTAATCAGATGGATGGGTTTGACCAGACAGTGAATGTTAAGGTTATTATGGCTACTAACCGAGCAGACACCTTGGATCCTGCACTTCTGCGTCCTGGACCATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005911 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0030054 GO:0030162 GO:0030163 GO:0030312 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055044 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

330

Amino Acids

36.55

Weight (kDa)

5.01

Isoelectric Point (pI)

34.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 492
AccB1I GGYRCC 1 cut(s) 259
AccBSI CCGCTC 1 cut(s) 265
AccI GTMKAC 2 cut(s) 294, 426
AccII CGCG 2 cut(s) 216, 228
AccIII TCCGGA 1 cut(s) 439
AciI CCGC 7 cut(s) 133, 151, 226, 263, 387, 406, 545
AclWI GGATC 8 cut(s) 20, 33, 66, 244, 257, 590, 953, 966
AcsI RAATTY 2 cut(s) 534, 707
AcuI CTGAAG 2 cut(s) 221, 230
AcvI CACGTG 1 cut(s) 605
AcyI GRCGYC 1 cut(s) 489
AdeI CACNNNGTG 1 cut(s) 608
AfaI GTAC 2 cut(s) 185, 747
AfiI CCNNNNNNNGG 6 cut(s) 150, 232, 238, 366, 605, 635
AgeI ACCGGT 1 cut(s) 635
AjnI CCWGG 6 cut(s) 81, 102, 174, 231, 628, 976
AluBI AGCT 6 cut(s) 157, 199, 220, 373, 848, 880
AluI AGCT 6 cut(s) 157, 199, 220, 373, 848, 880
Alw21I GWGCWC 3 cut(s) 222, 375, 882
AlwI GGATC 8 cut(s) 20, 33, 66, 244, 257, 590, 953, 966
Ama87I CYCGRG 2 cut(s) 158, 451
Aor13HI TCCGGA 1 cut(s) 439
AoxI GGCC 2 cut(s) 105, 444
ApeKI GCWGC 2 cut(s) 154, 680
ApoI RAATTY 2 cut(s) 534, 707
AseI ATTAAT 1 cut(s) 363
AsiGI ACCGGT 1 cut(s) 635
Asp700I GAANNNNTTC 1 cut(s) 711
AspLEI GCGC 2 cut(s) 216, 230
AspS9I GGNCC 3 cut(s) 623, 734, 980
AsuHPI GGTGA 3 cut(s) 52, 484, 557
AvaI CYCGRG 2 cut(s) 158, 451
AvaII GGWCC 3 cut(s) 623, 734, 980
BamHI GGATCC 3 cut(s) 25, 249, 958
BanI GGYRCC 1 cut(s) 259
BanII GRGCYC 4 cut(s) 222, 320, 375, 882
BauI CACGAG 1 cut(s) 569
BbrPI CACGTG 1 cut(s) 605
Bbv12I GWGCWC 3 cut(s) 222, 375, 882
BbvCI CCTCAGC 1 cut(s) 467
BbvI GCAGC 2 cut(s) 166, 667
BccI CCATC 4 cut(s) 283, 794, 886, 890
BcgI CGANNNNNNTGC 4 cut(s) 19, 53, 518, 552
BciT130I CCWGG 6 cut(s) 83, 104, 176, 233, 630, 978
BfaI CTAG 1 cut(s) 825
BfmI CTRYAG 1 cut(s) 127
BisI GCNGC 5 cut(s) 133, 152, 155, 263, 681
BlpI GCTNAGC 1 cut(s) 10
BlsI GCNGC 5 cut(s) 134, 153, 156, 264, 682
Bme1390I CCNGG 6 cut(s) 83, 104, 176, 233, 630, 978
Bme18I GGWCC 3 cut(s) 623, 734, 980
BmeT110I CYCGRG 2 cut(s) 158, 451
BmgT120I GGNCC 3 cut(s) 623, 734, 980
BmiI GGNNCC 9 cut(s) 27, 45, 251, 261, 319, 331, 634, 735, 960
BmrFI CCNGG 6 cut(s) 83, 104, 176, 233, 630, 978
BmsI GCATC 3 cut(s) 62, 799, 823
BplI GAGNNNNNCTC 4 cut(s) 434, 466, 445, 477
BpmI CTGGAG 2 cut(s) 65, 864
Bpu10I CCTNAGC 1 cut(s) 467
Bpu1102I GCTNAGC 1 cut(s) 10
BpuEI CTTGAG 1 cut(s) 362
Bsa29I ATCGAT 1 cut(s) 56
BsaAI YACGTR 2 cut(s) 605, 749
BsaHI GRCGYC 1 cut(s) 489
BsaJI CCNNGG 5 cut(s) 15, 82, 231, 768, 954
BsaWI WCCGGW 2 cut(s) 439, 635
BsaXI ACNNNNNCTCC 2 cut(s) 395, 425
Bsc4I CCNNNNNNNGG 6 cut(s) 150, 232, 238, 366, 605, 635
Bse118I RCCGGY 3 cut(s) 49, 326, 635
Bse1I ACTGG 2 cut(s) 472, 847
BseAI TCCGGA 1 cut(s) 439
BseBI CCWGG 6 cut(s) 83, 104, 176, 233, 630, 978
BseCI ATCGAT 1 cut(s) 56
BseDI CCNNGG 5 cut(s) 15, 82, 231, 768, 954
BseGI GGATG 4 cut(s) 171, 518, 814, 901
BseLI CCNNNNNNNGG 6 cut(s) 150, 232, 238, 366, 605, 635
BseMII CTCAG 4 cut(s) 24, 367, 468, 481
BseNI ACTGG 2 cut(s) 472, 847
BseRI GAGGAG 5 cut(s) 194, 212, 251, 365, 455
BseXI GCAGC 2 cut(s) 166, 667
BsgI GTGCAG 1 cut(s) 948
Bsh1236I CGCG 2 cut(s) 216, 228
BshFI GGCC 2 cut(s) 107, 446
BshNI GGYRCC 1 cut(s) 259
BshTI ACCGGT 1 cut(s) 635
BshVI ATCGAT 1 cut(s) 56
BsiHKAI GWGCWC 3 cut(s) 222, 375, 882
BsiHKCI CYCGRG 2 cut(s) 158, 451
BsiSI CCGG 5 cut(s) 41, 50, 327, 440, 636
BslI CCNNNNNNNGG 6 cut(s) 150, 232, 238, 366, 605, 635
BsmI GAATGC 1 cut(s) 781
BsnI GGCC 2 cut(s) 107, 446
BsoBI CYCGRG 2 cut(s) 158, 451
Bsp1286I GDGCHC 4 cut(s) 222, 320, 375, 882
Bsp13I TCCGGA 1 cut(s) 439
Bsp143I GATC 7 cut(s) 25, 57, 71, 121, 249, 595, 958
Bsp1720I GCTNAGC 1 cut(s) 10
Bsp19I CCATGG 1 cut(s) 15
BspACI CCGC 7 cut(s) 133, 151, 226, 263, 387, 406, 545
BspANI GGCC 2 cut(s) 107, 446
BspCNI CTCAG 4 cut(s) 23, 366, 469, 480
BspDI ATCGAT 1 cut(s) 56
BspEI TCCGGA 1 cut(s) 439
BspFNI CGCG 2 cut(s) 216, 228
BspLI GGNNCC 9 cut(s) 27, 45, 251, 261, 319, 331, 634, 735, 960
BspPI GGATC 8 cut(s) 20, 33, 66, 244, 257, 590, 953, 966
BspT107I GGYRCC 1 cut(s) 259
BsrBI CCGCTC 1 cut(s) 265
BsrFI RCCGGY 3 cut(s) 49, 326, 635
BsrI ACTGG 2 cut(s) 472, 847
BssAI RCCGGY 3 cut(s) 49, 326, 635
BssECI CCNNGG 5 cut(s) 15, 82, 231, 768, 954
BssMI GATC 7 cut(s) 25, 57, 71, 121, 249, 595, 958
BssNI GRCGYC 1 cut(s) 489
BssSI CACGAG 1 cut(s) 569
BssT1I CCWWGG 3 cut(s) 15, 768, 954
Bst2BI CACGAG 1 cut(s) 569
Bst2UI CCWGG 6 cut(s) 83, 104, 176, 233, 630, 978
Bst4CI ACNGT 1 cut(s) 913
BstACI GRCGYC 1 cut(s) 489
BstBAI YACGTR 2 cut(s) 605, 749
BstC8I GCNNGC 2 cut(s) 7, 218
BstDEI CTNAG 4 cut(s) 10, 353, 467, 477
BstDSI CCRYGG 1 cut(s) 15
BstF5I GGATG 4 cut(s) 171, 518, 814, 901
BstFNI CGCG 2 cut(s) 216, 228
BstHHI GCGC 2 cut(s) 216, 230
BstKTI GATC 7 cut(s) 28, 60, 74, 124, 252, 598, 961
BstMBI GATC 7 cut(s) 25, 57, 71, 121, 249, 595, 958
BstMWI GCNNNNNNNGC 3 cut(s) 11, 379, 395
BstNI CCWGG 6 cut(s) 83, 104, 176, 233, 630, 978
BstSCI CCNGG 6 cut(s) 81, 102, 174, 231, 628, 976
BstSFI CTRYAG 1 cut(s) 127
BstUI CGCG 2 cut(s) 216, 228
BstV1I GCAGC 2 cut(s) 166, 667
BstX2I RGATCY 5 cut(s) 25, 71, 249, 595, 958
BstXI CCANNNNNNTGG 1 cut(s) 22
BstYI RGATCY 5 cut(s) 25, 71, 249, 595, 958
Bsu15I ATCGAT 1 cut(s) 56
BsuRI GGCC 2 cut(s) 107, 446
BsuTUI ATCGAT 1 cut(s) 56
BtgI CCRYGG 1 cut(s) 15
BtsCI GGATG 4 cut(s) 171, 518, 814, 901
BtsIMutI CAGTG 1 cut(s) 918
Cac8I GCNNGC 2 cut(s) 7, 218
CfoI GCGC 2 cut(s) 216, 230
Cfr10I RCCGGY 3 cut(s) 49, 326, 635
Cfr13I GGNCC 3 cut(s) 623, 734, 980
ClaI ATCGAT 1 cut(s) 56
CseI GACGC 1 cut(s) 962
Csp6I GTAC 2 cut(s) 184, 746
CspAI ACCGGT 1 cut(s) 635
CviAII CATG 3 cut(s) 16, 283, 646
CviQI GTAC 2 cut(s) 184, 746
DdeI CTNAG 4 cut(s) 10, 353, 467, 477
DpnI GATC 7 cut(s) 27, 59, 73, 123, 251, 597, 960
DpnII GATC 7 cut(s) 25, 57, 71, 121, 249, 595, 958
DraIII CACNNNGTG 1 cut(s) 608
Ecl136II GAGCTC 3 cut(s) 220, 373, 880
Eco130I CCWWGG 3 cut(s) 15, 768, 954
Eco147I AGGCCT 1 cut(s) 107
Eco24I GRGCYC 4 cut(s) 222, 320, 375, 882
Eco47I GGWCC 3 cut(s) 623, 734, 980
Eco53kI GAGCTC 3 cut(s) 220, 373, 880
Eco57I CTGAAG 2 cut(s) 221, 230
Eco72I CACGTG 1 cut(s) 605
Eco88I CYCGRG 2 cut(s) 158, 451
EcoICRI GAGCTC 3 cut(s) 220, 373, 880
EcoRII CCWGG 6 cut(s) 81, 102, 174, 231, 628, 976
EcoT14I CCWWGG 3 cut(s) 15, 768, 954
EcoT38I GRGCYC 4 cut(s) 222, 320, 375, 882
ErhI CCWWGG 3 cut(s) 15, 768, 954
FaeI CATG 3 cut(s) 19, 286, 649
FatI CATG 3 cut(s) 15, 282, 645
FblI GTMKAC 2 cut(s) 294, 426
Fnu4HI GCNGC 5 cut(s) 133, 152, 155, 263, 681
FokI GGATG 4 cut(s) 158, 525, 821, 908
FriOI GRGCYC 4 cut(s) 222, 320, 375, 882
Fsp4HI GCNGC 5 cut(s) 133, 152, 155, 263, 681
FspBI CTAG 1 cut(s) 825
GlaI GCGC 2 cut(s) 215, 229
GluI GCNGC 5 cut(s) 133, 152, 155, 263, 681
GsuI CTGGAG 2 cut(s) 65, 864
HaeIII GGCC 2 cut(s) 107, 446
HapII CCGG 5 cut(s) 41, 50, 327, 440, 636
HgaI GACGC 1 cut(s) 962
HhaI GCGC 2 cut(s) 216, 230
Hin1I GRCGYC 1 cut(s) 489
Hin1II CATG 3 cut(s) 19, 286, 649
Hin6I GCGC 2 cut(s) 214, 228
HinP1I GCGC 2 cut(s) 214, 228
HincII GTYRAC 2 cut(s) 295, 427
HindII GTYRAC 2 cut(s) 295, 427
HinfI GANTC 4 cut(s) 205, 349, 449, 867
HpaII CCGG 5 cut(s) 41, 50, 327, 440, 636
HphI GGTGA 3 cut(s) 52, 484, 557
Hpy166II GTNNAC 4 cut(s) 295, 427, 626, 737
Hpy188III TCNNGA 4 cut(s) 244, 368, 440, 539
Hpy8I GTNNAC 4 cut(s) 295, 427, 626, 737
Hpy99I CGWCG 5 cut(s) 116, 398, 428, 431, 491
HpyAV CCTTC 3 cut(s) 393, 649, 694
HpyCH4III ACNGT 1 cut(s) 913
HpyCH4IV ACGT 5 cut(s) 186, 429, 489, 604, 748
HpyCH4V TGCA 2 cut(s) 403, 965
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 379, 395
HpyF3I CTNAG 4 cut(s) 10, 353, 467, 477
HpySE526I ACGT 5 cut(s) 186, 429, 489, 604, 748
Hsp92I GRCGYC 1 cut(s) 489
Hsp92II CATG 3 cut(s) 19, 286, 649
HspAI GCGC 2 cut(s) 214, 228
Kpn2I TCCGGA 1 cut(s) 439
Kzo9I GATC 7 cut(s) 25, 57, 71, 121, 249, 595, 958
LmnI GCTCC 9 cut(s) 225, 323, 335, 370, 378, 784, 845, 877, 885
Lsp1109I GCAGC 2 cut(s) 166, 667
LweI GCATC 3 cut(s) 62, 799, 823
MaeI CTAG 1 cut(s) 825
MaeII ACGT 5 cut(s) 186, 429, 489, 604, 748
MaeIII GTNAC 2 cut(s) 490, 515
MalI GATC 7 cut(s) 27, 59, 73, 123, 251, 597, 960
MbiI CCGCTC 1 cut(s) 265
MboI GATC 7 cut(s) 25, 57, 71, 121, 249, 595, 958
MboII GAAGA 1 cut(s) 214
MflI RGATCY 5 cut(s) 25, 71, 249, 595, 958
MhlI GDGCHC 4 cut(s) 222, 320, 375, 882
MluCI AATT 3 cut(s) 334, 534, 707
MlyI GAGTC 1 cut(s) 443
MmeI TCCRAC 2 cut(s) 99, 135
MroI TCCGGA 1 cut(s) 439
MroXI GAANNNNTTC 1 cut(s) 711
MseI TTAA 3 cut(s) 363, 885, 921
MslI CAYNNNNRTG 2 cut(s) 287, 414
MspA1I CMGCKG 1 cut(s) 151
MspI CCGG 5 cut(s) 41, 50, 327, 440, 636
MspR9I CCNGG 6 cut(s) 83, 104, 176, 233, 630, 978
Mva1269I GAATGC 1 cut(s) 781
MvaI CCWGG 6 cut(s) 83, 104, 176, 233, 630, 978
MvnI CGCG 2 cut(s) 216, 228
MwoI GCNNNNNNNGC 3 cut(s) 11, 379, 395
NcoI CCATGG 1 cut(s) 15
NdeII GATC 7 cut(s) 25, 57, 71, 121, 249, 595, 958
NlaIII CATG 3 cut(s) 19, 286, 649
NlaIV GGNNCC 9 cut(s) 27, 45, 251, 261, 319, 331, 634, 735, 960
NmuCI GTSAC 2 cut(s) 490, 515
PaeR7I CTCGAG 2 cut(s) 158, 451
PceI AGGCCT 1 cut(s) 107
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 192, 745
PctI GAATGC 1 cut(s) 781
PdmI GAANNNNTTC 1 cut(s) 711
PfeI GAWTC 3 cut(s) 205, 349, 867
PfoI TCCNGGA 2 cut(s) 174, 976
PinAI ACCGGT 1 cut(s) 635
PkrI GCNGC 5 cut(s) 134, 153, 156, 264, 682
PleI GAGTC 1 cut(s) 443
PmaCI CACGTG 1 cut(s) 605
PmlI CACGTG 1 cut(s) 605
PpsI GAGTC 1 cut(s) 443
Ppu21I YACGTR 2 cut(s) 605, 749
PshBI ATTAAT 1 cut(s) 363
Psp124BI GAGCTC 3 cut(s) 222, 375, 882
Psp6I CCWGG 6 cut(s) 81, 102, 174, 231, 628, 976
PspCI CACGTG 1 cut(s) 605
PspGI CCWGG 6 cut(s) 81, 102, 174, 231, 628, 976
PspN4I GGNNCC 9 cut(s) 27, 45, 251, 261, 319, 331, 634, 735, 960
PspPI GGNCC 3 cut(s) 623, 734, 980
PspXI VCTCGAGB 2 cut(s) 158, 451
PsuI RGATCY 5 cut(s) 25, 71, 249, 595, 958
RsaI GTAC 2 cut(s) 185, 747
RsaNI GTAC 2 cut(s) 184, 746
RseI CAYNNNNRTG 2 cut(s) 287, 414
SacI GAGCTC 3 cut(s) 222, 375, 882
SalI GTCGAC 2 cut(s) 293, 425
SaqAI TTAA 3 cut(s) 363, 885, 921
SatI GCNGC 5 cut(s) 133, 152, 155, 263, 681
Sau3AI GATC 7 cut(s) 25, 57, 71, 121, 249, 595, 958
Sau96I GGNCC 3 cut(s) 623, 734, 980
SchI GAGTC 1 cut(s) 443
ScrFI CCNGG 6 cut(s) 83, 104, 176, 233, 630, 978
SduI GDGCHC 4 cut(s) 222, 320, 375, 882
SfaNI GCATC 3 cut(s) 62, 799, 823
SfcI CTRYAG 1 cut(s) 127
Sfr274I CTCGAG 2 cut(s) 158, 451
SgrDI CGTCGACG 1 cut(s) 425
SinI GGWCC 3 cut(s) 623, 734, 980
SlaI CTCGAG 2 cut(s) 158, 451
SmiMI CAYNNNNRTG 2 cut(s) 287, 414
SmlI CTYRAG 3 cut(s) 158, 377, 451
SmoI CTYRAG 3 cut(s) 158, 377, 451
Sse9I AATT 3 cut(s) 334, 534, 707
SseBI AGGCCT 1 cut(s) 107
SsiI CCGC 7 cut(s) 133, 151, 226, 263, 387, 406, 545
SspMI CTAG 1 cut(s) 825
SstI GAGCTC 3 cut(s) 222, 375, 882
StuI AGGCCT 1 cut(s) 107
StyD4I CCNGG 6 cut(s) 81, 102, 174, 231, 628, 976
StyI CCWWGG 3 cut(s) 15, 768, 954
TaaI ACNGT 1 cut(s) 913
TaiI ACGT 5 cut(s) 189, 432, 492, 607, 751
TaqI TCGA 6 cut(s) 56, 159, 168, 294, 426, 452
TasI AATT 3 cut(s) 334, 534, 707
TauI GCSGC 3 cut(s) 135, 154, 265
TfiI GAWTC 3 cut(s) 205, 349, 867
Tru1I TTAA 3 cut(s) 363, 885, 921
Tru9I TTAA 3 cut(s) 363, 885, 921
TscAI CASTG 1 cut(s) 918
TseFI GTSAC 2 cut(s) 490, 515
TseI GCWGC 2 cut(s) 154, 680
Tsp45I GTSAC 2 cut(s) 490, 515
TspDTI ATGAA 3 cut(s) 154, 271, 676
TspGWI ACGGA 2 cut(s) 382, 749
TspRI CASTG 1 cut(s) 918
VpaK11BI GGWCC 3 cut(s) 623, 734, 980
VspI ATTAAT 1 cut(s) 363
XapI RAATTY 2 cut(s) 534, 707
XhoI CTCGAG 2 cut(s) 158, 451
XmiI GTMKAC 2 cut(s) 294, 426
XmnI GAANNNNTTC 1 cut(s) 711
XspI CTAG 1 cut(s) 825
ZraI GACGTC 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.