RchiOBHm_Chr5g0027211

26S protease regulatory subunit 6B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002106
Physical Location & Seq
Forward (+)
21204440 .. 21205851
1412 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30676

Sequence Viewer

Length: 474 bp
ATGTTCTGCTGCCGGAGGCCGACTCCAATATTTCGCTGCTCAGCCAGAACGAGAAGCCTGACGTCACCTATAATCACTTCGAGGAGAGATATCGGGGGATGCGACATTCAAAAGCAAGAAATCCATGAAGCAGTGGAACTTCCACTAACACACCATGACTTATACAAACAGATTGGAATAGATCCTCCACGTGGTGTATTGCTGTATGGTCCACCTGGAACTGGTAAAATCATGCTAGCTAAGGCTGTTGCCAATCATACAACTACTGCCTTCATTAGAGTTGTTGGTTCAGAATTTGTTCAAAAGTATTTGGGTGAGGTTCCACGAATGGTCCGTGATGTTTTCCATCTCGCCAAGGAGAATGCACCTACCATCATCTTTATTGATGAGGTGGATGCTATTGCTACTGCAAGGTTTGATGCTCAAACTGGAGCTGATAGAAAAGAAAATATTTTTTGTGCAGTAATGACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005911 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0030054 GO:0030162 GO:0030163 GO:0030312 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055044 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

157

Amino Acids

17.48

Weight (kDa)

8.67

Isoelectric Point (pI)

35.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF815 PF05673 62 - 131 2.8e-06 Protein of unknown function (DUF815)
AAA PF00004 66 - 149 1e-25 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 65
AclWI GGATC 1 cut(s) 176
AcsI RAATTY 1 cut(s) 293
AcvI CACGTG 1 cut(s) 191
AcyI GRCGYC 1 cut(s) 62
AdeI CACNNNGTG 1 cut(s) 194
AfiI CCNNNNNNNGG 2 cut(s) 191, 221
AgsI TTSAA 2 cut(s) 110, 302
AjnI CCWGG 1 cut(s) 214
AluBI AGCT 2 cut(s) 239, 434
AluI AGCT 2 cut(s) 239, 434
AlwI GGATC 1 cut(s) 176
AoxI GGCC 1 cut(s) 17
ApeKI GCWGC 2 cut(s) 9, 36
ApoI RAATTY 1 cut(s) 293
Asp700I GAANNNNTTC 1 cut(s) 297
AspS9I GGNCC 2 cut(s) 209, 331
AsuHPI GGTGA 2 cut(s) 57, 326
AsuNHI GCTAGC 1 cut(s) 235
AvaII GGWCC 2 cut(s) 209, 331
BbrPI CACGTG 1 cut(s) 191
BbvI GCAGC 1 cut(s) 23
BccI CCATC 2 cut(s) 354, 380
BciT130I CCWGG 1 cut(s) 216
BfaI CTAG 1 cut(s) 236
BisI GCNGC 2 cut(s) 10, 37
BlpI GCTNAGC 1 cut(s) 40
BlsI GCNGC 2 cut(s) 11, 38
Bme1390I CCNGG 1 cut(s) 216
Bme18I GGWCC 2 cut(s) 209, 331
BmgT120I GGNCC 2 cut(s) 209, 331
BmiI GGNNCC 1 cut(s) 321
BmrFI CCNGG 1 cut(s) 216
BmsI GCATC 3 cut(s) 89, 385, 409
BmtI GCTAGC 1 cut(s) 239
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 1 cut(s) 450
Bpu10I CCTNAGC 1 cut(s) 240
Bpu1102I GCTNAGC 1 cut(s) 40
BsaAI YACGTR 1 cut(s) 191
BsaHI GRCGYC 1 cut(s) 62
BsaJI CCNNGG 1 cut(s) 354
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 221
Bse1I ACTGG 2 cut(s) 226, 433
BseBI CCWGG 1 cut(s) 216
BseDI CCNNGG 1 cut(s) 354
BseGI GGATG 2 cut(s) 104, 400
BseLI CCNNNNNNNGG 2 cut(s) 191, 221
BseMII CTCAG 1 cut(s) 54
BseNI ACTGG 2 cut(s) 226, 433
BseRI GAGGAG 1 cut(s) 97
BseXI GCAGC 1 cut(s) 23
BshFI GGCC 1 cut(s) 19
BsiSI CCGG 1 cut(s) 13
BslI CCNNNNNNNGG 2 cut(s) 191, 221
BsmI GAATGC 1 cut(s) 367
BsnI GGCC 1 cut(s) 19
Bsp143I GATC 1 cut(s) 181
Bsp1720I GCTNAGC 1 cut(s) 40
BspANI GGCC 1 cut(s) 19
BspCNI CTCAG 1 cut(s) 53
BspLI GGNNCC 1 cut(s) 321
BspOI GCTAGC 1 cut(s) 239
BspPI GGATC 1 cut(s) 176
BsrI ACTGG 2 cut(s) 226, 433
BssECI CCNNGG 1 cut(s) 354
BssMI GATC 1 cut(s) 181
BssNI GRCGYC 1 cut(s) 62
BssT1I CCWWGG 1 cut(s) 354
Bst2UI CCWGG 1 cut(s) 216
BstACI GRCGYC 1 cut(s) 62
BstBAI YACGTR 1 cut(s) 191
BstC8I GCNNGC 1 cut(s) 237
BstDEI CTNAG 2 cut(s) 40, 240
BstF5I GGATG 2 cut(s) 104, 400
BstKTI GATC 1 cut(s) 184
BstMBI GATC 1 cut(s) 181
BstNI CCWGG 1 cut(s) 216
BstSCI CCNGG 1 cut(s) 214
BstV1I GCAGC 1 cut(s) 23
BstX2I RGATCY 1 cut(s) 181
BstYI RGATCY 1 cut(s) 181
BsuRI GGCC 1 cut(s) 19
BtsCI GGATG 2 cut(s) 104, 400
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 1 cut(s) 138
Cac8I GCNNGC 1 cut(s) 237
Cfr13I GGNCC 2 cut(s) 209, 331
CviAII CATG 3 cut(s) 125, 155, 232
CviJI RGCY 6 cut(s) 19, 44, 57, 239, 245, 434
CviKI_1 RGCY 6 cut(s) 19, 44, 57, 239, 245, 434
DdeI CTNAG 2 cut(s) 40, 240
DpnI GATC 1 cut(s) 183
DpnII GATC 1 cut(s) 181
DraIII CACNNNGTG 1 cut(s) 194
Eco130I CCWWGG 1 cut(s) 354
Eco32I GATATC 1 cut(s) 91
Eco47I GGWCC 2 cut(s) 209, 331
Eco72I CACGTG 1 cut(s) 191
EcoRII CCWGG 1 cut(s) 214
EcoRV GATATC 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 354
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 3 cut(s) 128, 158, 235
FaiI YATR 8 cut(s) 71, 126, 156, 163, 207, 233, 258, 472
FatI CATG 3 cut(s) 124, 154, 231
Fnu4HI GCNGC 2 cut(s) 10, 37
FokI GGATG 2 cut(s) 111, 407
Fsp4HI GCNGC 2 cut(s) 10, 37
FspBI CTAG 1 cut(s) 236
GluI GCNGC 2 cut(s) 10, 37
GsuI CTGGAG 1 cut(s) 450
HaeIII GGCC 1 cut(s) 19
HapII CCGG 1 cut(s) 13
Hin1I GRCGYC 1 cut(s) 62
Hin1II CATG 3 cut(s) 128, 158, 235
HinfI GANTC 1 cut(s) 22
HpaII CCGG 1 cut(s) 13
HphI GGTGA 2 cut(s) 57, 326
Hpy166II GTNNAC 1 cut(s) 212
Hpy188I TCNGA 1 cut(s) 292
Hpy8I GTNNAC 1 cut(s) 212
HpyAV CCTTC 1 cut(s) 280
HpyCH4IV ACGT 2 cut(s) 62, 190
HpyCH4V TGCA 3 cut(s) 365, 410, 461
HpyF3I CTNAG 2 cut(s) 40, 240
HpySE526I ACGT 2 cut(s) 62, 190
Hsp92I GRCGYC 1 cut(s) 62
Hsp92II CATG 3 cut(s) 128, 158, 235
Kzo9I GATC 1 cut(s) 181
LmnI GCTCC 1 cut(s) 431
LpnPI CCDG 7 cut(s) 26, 58, 71, 201, 207, 228, 414
Lsp1109I GCAGC 1 cut(s) 23
LweI GCATC 3 cut(s) 89, 385, 409
MaeI CTAG 1 cut(s) 236
MaeII ACGT 2 cut(s) 62, 190
MaeIII GTNAC 1 cut(s) 63
MalI GATC 1 cut(s) 183
MboI GATC 1 cut(s) 181
MflI RGATCY 1 cut(s) 181
MluCI AATT 1 cut(s) 293
MlyI GAGTC 1 cut(s) 16
MnlI CCTC 5 cut(s) 9, 75, 195, 310, 382
MroXI GAANNNNTTC 1 cut(s) 297
MspI CCGG 1 cut(s) 13
MspR9I CCNGG 1 cut(s) 216
Mva1269I GAATGC 1 cut(s) 367
MvaI CCWGG 1 cut(s) 216
NdeII GATC 1 cut(s) 181
NheI GCTAGC 1 cut(s) 235
NlaIII CATG 3 cut(s) 128, 158, 235
NlaIV GGNNCC 1 cut(s) 321
NmuCI GTSAC 1 cut(s) 63
PcsI WCGNNNNNNNCGW 2 cut(s) 99, 331
PctI GAATGC 1 cut(s) 367
PdmI GAANNNNTTC 1 cut(s) 297
PkrI GCNGC 2 cut(s) 11, 38
PleI GAGTC 1 cut(s) 16
PmaCI CACGTG 1 cut(s) 191
PmlI CACGTG 1 cut(s) 191
PpsI GAGTC 1 cut(s) 16
Ppu21I YACGTR 1 cut(s) 191
Psp6I CCWGG 1 cut(s) 214
PspCI CACGTG 1 cut(s) 191
PspGI CCWGG 1 cut(s) 214
PspN4I GGNNCC 1 cut(s) 321
PspPI GGNCC 2 cut(s) 209, 331
PsuI RGATCY 1 cut(s) 181
SatI GCNGC 2 cut(s) 10, 37
Sau3AI GATC 1 cut(s) 181
Sau96I GGNCC 2 cut(s) 209, 331
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 1 cut(s) 216
SfaNI GCATC 3 cut(s) 89, 385, 409
SinI GGWCC 2 cut(s) 209, 331
Sse9I AATT 1 cut(s) 293
SspI AATATT 2 cut(s) 30, 451
SspMI CTAG 1 cut(s) 236
StyD4I CCNGG 1 cut(s) 214
StyI CCWWGG 1 cut(s) 354
TaiI ACGT 2 cut(s) 65, 193
TaqI TCGA 1 cut(s) 80
TasI AATT 1 cut(s) 293
TscAI CASTG 1 cut(s) 138
TseFI GTSAC 1 cut(s) 63
TseI GCWGC 2 cut(s) 9, 36
Tsp45I GTSAC 1 cut(s) 63
TspDTI ATGAA 2 cut(s) 141, 262
TspGWI ACGGA 1 cut(s) 323
TspRI CASTG 1 cut(s) 138
VpaK11BI GGWCC 2 cut(s) 209, 331
XapI RAATTY 1 cut(s) 293
XmnI GAANNNNTTC 1 cut(s) 297
XspI CTAG 1 cut(s) 236
ZraI GACGTC 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.