RchiOBHm_Chr5g0073581

26S protease regulatory subunit 6B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002106
Physical Location & Seq
Reverse (-)
79585068 .. 79588586
3519 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34846

Sequence Viewer

Length: 1254 bp
ATGGCATCGGCGATGGTTCTGGACCCGAAACCCCTACCAGAGCCACCGGCTTCGCTGCCCTCCCTCAGATCCGACCCGGCCTTCACCGACCCGAACTCCGACGAAGACTCCGATCTCTACAGCCGCCTCAAGGCCCTTCAGCGCCAGAACGAGTTCATCGACATCAAGGAGGAGTACGTCAAAGACGAGCAGAAGAACCTGAAGCGCGAGATGCTGCGGTCGCAGGAGGAGGTGAAGCGGATCCAGTCGGTGCCGCTGGTGATCGGCCAGTTTCTCGAGATGGTCGACGAGAACCACGGCATTGTGGGCTCCACCACGGGCTCGAATTACTACGTGAGGATTCTGAGCACGATTAACCGGGAGCACCTGAAGCCGTCGGCGTCGGTGGCGTTGCACCGCCACTCCAATGCTCTTGTGGATGTTCTGCCGCCGGAGGCGGACTCGAGTATCTCGCTGCTCAGCCAGTCGGAGAAGCCCGACGTCACCTACAGTGATATTGGAGGATGCGATATTCAGAAGCAAGAAATCCGTGAAGCAGTGGAACTTCCACTTACTCACCATGACTTATACAAACAGATTGGAATAGATCCTCCACGTGGTGTATTGCTCTATGGCCCACCTGGAACTGGTAAAACCATGCTAGCTAAGGCTGTGGCTAACCATACAACTGCTGCTTTCATTAGAGTTGTTGGTTCAGAATTTGTTCAGAAGTATTTGGGTGAGGGTCCACGAATGGTTCGTGATGTTTTCCGCCTTGCCAAGGAGAATGCACCTGCCATCATCTTTATTGATGAGGTTGATGCTATTGCTACTGCAAGGTTTGATGCTCAAACTGGAGCTGATAGAGAAGTTCAACGAATCCTAATGGAGCTCCTCAATCAGATGGATGGGTTTGACCAGACTGTAAACGTTAAGGTCATAATGGCTACTAATCGTGCAGACACTTTGGATCCTGCACTTCTGCGTCCCGGAAGGCTTGACCGTAAGATTGAATTTCCTTTGCCTGATAGGCGTCAGAAAAGGCTTGTTTTTCAGGTTTGCACTGCTAAAATGAACTTGAGTGATGAGGTAGACTTGGAAGATTATGTATCTCGGCCAGATAAAATCAGCGCTGCTGAGATTACAGCTATCTGTCAAGAAGCCGGAATGCACGCAGTTCGCAAGAACCGATATGTCATACTCCCAAAGGACTTTGAAAAGGGTTATCAAACCAATGTGAAGAAGCCTGATACTGACTTTGAATTCTACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005911 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0030054 GO:0030162 GO:0030163 GO:0030312 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055044 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

417

Amino Acids

46.9

Weight (kDa)

5.47

Isoelectric Point (pI)

38.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_ATP_ID_OB_C PF16450 88 - 143 2.9e-12 Proteasomal ATPase OB C-terminal domain
AAA_5 PF07728 200 - 322 6.7e-08 AAA domain (dynein-related subfamily)
AAA PF00004 201 - 334 2.1e-45 ATPase family associated with various cellular activities (AAA)
AAA_2 PF07724 201 - 294 2.9e-06 AAA domain (Cdc48 subfamily)
AAA_lid_3 PF17862 357 - 400 6.8e-07 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 781
AatII GACGTC 1 cut(s) 483
Acc36I ACCTGC 1 cut(s) 781
AccB1I GGYRCC 1 cut(s) 250
AccI GTMKAC 2 cut(s) 285, 1071
AccII CGCG 1 cut(s) 207
AciI CCGC 8 cut(s) 124, 217, 238, 254, 397, 428, 437, 751
AclI AACGTT 1 cut(s) 909
AclWI GGATC 6 cut(s) 63, 235, 248, 581, 944, 957
AcoI YGGCCR 2 cut(s) 265, 1094
AcsI RAATTY 3 cut(s) 698, 992, 1241
AcuI CTGAAG 3 cut(s) 122, 221, 389
AcvI CACGTG 1 cut(s) 596
AcyI GRCGYC 3 cut(s) 380, 480, 1012
AdeI CACNNNGTG 1 cut(s) 599
AfaI GTAC 1 cut(s) 176
AfeI AGCGCT 1 cut(s) 1111
AfiI CCNNNNNNNGG 4 cut(s) 130, 596, 626, 760
AgsI TTSAA 4 cut(s) 854, 992, 1196, 1241
AjnI CCWGG 1 cut(s) 619
AluBI AGCT 4 cut(s) 644, 839, 871, 1127
AluI AGCT 4 cut(s) 644, 839, 871, 1127
Alw21I GWGCWC 3 cut(s) 350, 366, 873
AlwI GGATC 6 cut(s) 63, 235, 248, 581, 944, 957
Ama87I CYCGRG 2 cut(s) 275, 442
Aor51HI AGCGCT 1 cut(s) 1111
AoxI GGCC 5 cut(s) 78, 132, 265, 613, 1094
ApeKI GCWGC 5 cut(s) 55, 214, 454, 671, 1112
ApoI RAATTY 3 cut(s) 698, 992, 1241
Asp700I GAANNNNTTC 2 cut(s) 152, 702
AspLEI GCGC 3 cut(s) 144, 207, 1112
AspS9I GGNCC 4 cut(s) 22, 133, 614, 725
AsuC2I CCSGG 3 cut(s) 77, 359, 969
AsuHPI GGTGA 6 cut(s) 76, 244, 271, 475, 548, 731
AsuNHI GCTAGC 1 cut(s) 640
AvaI CYCGRG 2 cut(s) 275, 442
AvaII GGWCC 2 cut(s) 22, 725
BaeI ACNNNNGTAYC 2 cut(s) 430, 463
BamHI GGATCC 2 cut(s) 240, 949
BanI GGYRCC 1 cut(s) 250
BanII GRGCYC 3 cut(s) 311, 323, 873
BbrPI CACGTG 1 cut(s) 596
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 3 cut(s) 350, 366, 873
BbvI GCAGC 5 cut(s) 42, 201, 441, 658, 1099
BccI CCATC 5 cut(s) 7, 274, 785, 877, 881
BceAI ACGGC 2 cut(s) 313, 358
BcgI CGANNNNNNTGC 2 cut(s) 1139, 1173
BciT130I CCWGG 1 cut(s) 621
BcnI CCSGG 3 cut(s) 77, 359, 969
BfaI CTAG 1 cut(s) 641
BfmI CTRYAG 2 cut(s) 118, 487
BfoI RGCGCY 2 cut(s) 145, 1113
BfuAI ACCTGC 1 cut(s) 781
BglI GCCNNNNNGGC 1 cut(s) 1009
BisI GCNGC 8 cut(s) 56, 124, 215, 254, 428, 455, 672, 1113
BlpI GCTNAGC 1 cut(s) 458
BlsI GCNGC 8 cut(s) 57, 125, 216, 255, 429, 456, 673, 1114
Bme1390I CCNGG 4 cut(s) 77, 359, 621, 969
Bme18I GGWCC 2 cut(s) 22, 725
BmeT110I CYCGRG 2 cut(s) 275, 442
BmgT120I GGNCC 4 cut(s) 22, 133, 614, 725
BmiI GGNNCC 6 cut(s) 24, 242, 252, 310, 726, 951
BmrFI CCNGG 4 cut(s) 77, 359, 621, 969
BmsI GCATC 5 cut(s) 14, 201, 494, 790, 814
BmtI GCTAGC 1 cut(s) 644
BpiI GAAGAC 1 cut(s) 111
BplI GAGNNNNNCTC 2 cut(s) 425, 457
BpmI CTGGAG 1 cut(s) 855
Bpu10I CCTNAGC 1 cut(s) 645
Bpu1102I GCTNAGC 1 cut(s) 458
BpuEI CTTGAG 2 cut(s) 113, 1078
BpuMI CCSGG 3 cut(s) 77, 359, 969
BsaAI YACGTR 2 cut(s) 334, 596
BsaHI GRCGYC 3 cut(s) 380, 480, 1012
BsaJI CCNNGG 3 cut(s) 295, 315, 759
BsaXI ACNNNNNCTCC 6 cut(s) 80, 92, 110, 122, 386, 416
Bsc4I CCNNNNNNNGG 4 cut(s) 130, 596, 626, 760
Bse118I RCCGGY 1 cut(s) 46
Bse1I ACTGG 5 cut(s) 244, 268, 463, 631, 838
BseBI CCWGG 1 cut(s) 621
BseDI CCNNGG 3 cut(s) 295, 315, 759
BseGI GGATG 3 cut(s) 424, 509, 892
BseLI CCNNNNNNNGG 4 cut(s) 130, 596, 626, 760
BseMII CTCAG 4 cut(s) 79, 335, 472, 1107
BseNI ACTGG 5 cut(s) 244, 268, 463, 631, 838
BseRI GAGGAG 3 cut(s) 185, 242, 863
BseXI GCAGC 5 cut(s) 42, 201, 441, 658, 1099
BsgI GTGCAG 2 cut(s) 939, 957
Bsh1236I CGCG 1 cut(s) 207
Bsh1285I CGRYCG 1 cut(s) 221
BshFI GGCC 5 cut(s) 80, 134, 267, 615, 1096
BshNI GGYRCC 1 cut(s) 250
BsiEI CGRYCG 1 cut(s) 221
BsiHKAI GWGCWC 3 cut(s) 350, 366, 873
BsiHKCI CYCGRG 2 cut(s) 275, 442
BsiSI CCGG 6 cut(s) 47, 77, 358, 431, 969, 1143
BslFI GGGAC 1 cut(s) 951
BslI CCNNNNNNNGG 4 cut(s) 130, 596, 626, 760
BsmFI GGGAC 1 cut(s) 951
BsmI GAATGC 2 cut(s) 772, 1152
BsnI GGCC 5 cut(s) 80, 134, 267, 615, 1096
BsoBI CYCGRG 2 cut(s) 275, 442
Bsp1286I GDGCHC 5 cut(s) 311, 323, 350, 366, 873
Bsp143I GATC 6 cut(s) 68, 112, 240, 261, 586, 949
Bsp1720I GCTNAGC 1 cut(s) 458
BspACI CCGC 8 cut(s) 124, 217, 238, 254, 397, 428, 437, 751
BspANI GGCC 5 cut(s) 80, 134, 267, 615, 1096
BspCNI CTCAG 4 cut(s) 78, 336, 471, 1108
BspFNI CGCG 1 cut(s) 207
BspLI GGNNCC 6 cut(s) 24, 242, 252, 310, 726, 951
BspMI ACCTGC 1 cut(s) 781
BspOI GCTAGC 1 cut(s) 644
BspPI GGATC 6 cut(s) 63, 235, 248, 581, 944, 957
BspT107I GGYRCC 1 cut(s) 250
BsrFI RCCGGY 1 cut(s) 46
BsrI ACTGG 5 cut(s) 244, 268, 463, 631, 838
BssAI RCCGGY 1 cut(s) 46
BssECI CCNNGG 3 cut(s) 295, 315, 759
BssMI GATC 6 cut(s) 68, 112, 240, 261, 586, 949
BssNI GRCGYC 3 cut(s) 380, 480, 1012
BssT1I CCWWGG 1 cut(s) 759
Bst2UI CCWGG 1 cut(s) 621
Bst4CI ACNGT 3 cut(s) 491, 904, 983
BstACI GRCGYC 3 cut(s) 380, 480, 1012
BstBAI YACGTR 2 cut(s) 334, 596
BstC8I GCNNGC 2 cut(s) 642, 1152
BstDEI CTNAG 5 cut(s) 65, 344, 458, 645, 1116
BstDSI CCRYGG 2 cut(s) 295, 315
BstENI CCTNNNNNAGG 1 cut(s) 758
BstF5I GGATG 3 cut(s) 424, 509, 892
BstFNI CGCG 1 cut(s) 207
BstH2I RGCGCY 2 cut(s) 145, 1113
BstHHI GCGC 3 cut(s) 144, 207, 1112
BstKTI GATC 6 cut(s) 71, 115, 243, 264, 589, 952
BstMBI GATC 6 cut(s) 68, 112, 240, 261, 586, 949
BstMCI CGRYCG 1 cut(s) 221
BstMWI GCNNNNNNNGC 6 cut(s) 211, 220, 306, 370, 386, 1009
BstNI CCWGG 1 cut(s) 621
BstSCI CCNGG 4 cut(s) 75, 357, 619, 967
BstSFI CTRYAG 2 cut(s) 118, 487
BstUI CGCG 1 cut(s) 207
BstV1I GCAGC 5 cut(s) 42, 201, 441, 658, 1099
BstV2I GAAGAC 1 cut(s) 111
BstX2I RGATCY 4 cut(s) 68, 240, 586, 949
BstYI RGATCY 4 cut(s) 68, 240, 586, 949
BsuRI GGCC 5 cut(s) 80, 134, 267, 615, 1096
BtgI CCRYGG 2 cut(s) 295, 315
BtgZI GCGATG 1 cut(s) 26
BtsCI GGATG 3 cut(s) 424, 509, 892
BtsI GCAGTG 2 cut(s) 543, 1041
BtsIMutI CAGTG 3 cut(s) 496, 543, 1041
BveI ACCTGC 1 cut(s) 781
Cac8I GCNNGC 2 cut(s) 642, 1152
CfoI GCGC 3 cut(s) 144, 207, 1112
Cfr10I RCCGGY 1 cut(s) 46
Cfr13I GGNCC 4 cut(s) 22, 133, 614, 725
CseI GACGC 3 cut(s) 369, 953, 1001
Csp6I GTAC 1 cut(s) 175
CviAII CATG 2 cut(s) 560, 637
CviQI GTAC 1 cut(s) 175
DdeI CTNAG 5 cut(s) 65, 344, 458, 645, 1116
DpnI GATC 6 cut(s) 70, 114, 242, 263, 588, 951
DpnII GATC 6 cut(s) 68, 112, 240, 261, 586, 949
DraIII CACNNNGTG 1 cut(s) 599
EaeI YGGCCR 2 cut(s) 265, 1094
EciI GGCGGA 2 cut(s) 452, 740
Ecl136II GAGCTC 1 cut(s) 871
Eco130I CCWWGG 1 cut(s) 759
Eco24I GRGCYC 3 cut(s) 311, 323, 873
Eco47I GGWCC 2 cut(s) 22, 725
Eco47III AGCGCT 1 cut(s) 1111
Eco53kI GAGCTC 1 cut(s) 871
Eco57I CTGAAG 3 cut(s) 122, 221, 389
Eco72I CACGTG 1 cut(s) 596
Eco88I CYCGRG 2 cut(s) 275, 442
EcoICRI GAGCTC 1 cut(s) 871
EcoNI CCTNNNNNAGG 1 cut(s) 758
EcoO109I RGGNCCY 1 cut(s) 133
EcoRI GAATTC 1 cut(s) 1241
EcoRII CCWGG 1 cut(s) 619
EcoT14I CCWWGG 1 cut(s) 759
EcoT38I GRGCYC 3 cut(s) 311, 323, 873
ErhI CCWWGG 1 cut(s) 759
FaeI CATG 2 cut(s) 563, 640
FaiI YATR 9 cut(s) 561, 568, 612, 638, 663, 920, 1086, 1173, 1178
FaqI GGGAC 1 cut(s) 951
FatI CATG 2 cut(s) 559, 636
FblI GTMKAC 2 cut(s) 285, 1071
Fnu4HI GCNGC 8 cut(s) 56, 124, 215, 254, 428, 455, 672, 1113
FokI GGATG 3 cut(s) 431, 516, 899
FriOI GRGCYC 3 cut(s) 311, 323, 873
Fsp4HI GCNGC 8 cut(s) 56, 124, 215, 254, 428, 455, 672, 1113
FspBI CTAG 1 cut(s) 641
GlaI GCGC 3 cut(s) 143, 206, 1111
GluI GCNGC 8 cut(s) 56, 124, 215, 254, 428, 455, 672, 1113
GsuI CTGGAG 1 cut(s) 855
HaeII RGCGCY 2 cut(s) 145, 1113
HaeIII GGCC 5 cut(s) 80, 134, 267, 615, 1096
HapII CCGG 6 cut(s) 47, 77, 358, 431, 969, 1143
HgaI GACGC 3 cut(s) 369, 953, 1001
HhaI GCGC 3 cut(s) 144, 207, 1112
Hin1I GRCGYC 3 cut(s) 380, 480, 1012
Hin1II CATG 2 cut(s) 563, 640
Hin6I GCGC 3 cut(s) 142, 205, 1110
HinP1I GCGC 3 cut(s) 142, 205, 1110
HincII GTYRAC 1 cut(s) 286
HindII GTYRAC 1 cut(s) 286
HinfI GANTC 4 cut(s) 107, 340, 440, 858
HpaII CCGG 6 cut(s) 47, 77, 358, 431, 969, 1143
HphI GGTGA 6 cut(s) 76, 244, 271, 475, 548, 731
Hpy166II GTNNAC 4 cut(s) 286, 728, 907, 1072
Hpy188III TCNNGA 5 cut(s) 20, 275, 277, 740, 1136
Hpy8I GTNNAC 4 cut(s) 286, 728, 907, 1072
Hpy99I CGWCG 5 cut(s) 104, 290, 379, 385, 482
HpyAV CCTTC 3 cut(s) 91, 146, 966
HpyCH4III ACNGT 3 cut(s) 491, 904, 983
HpyCH4IV ACGT 5 cut(s) 177, 333, 480, 595, 909
HpyCH4V TGCA 7 cut(s) 394, 770, 815, 938, 956, 1041, 1150
HpyF10VI GCNNNNNNNGC 6 cut(s) 211, 220, 306, 370, 386, 1009
HpyF3I CTNAG 5 cut(s) 65, 344, 458, 645, 1116
HpySE526I ACGT 5 cut(s) 177, 333, 480, 595, 909
Hsp92I GRCGYC 3 cut(s) 380, 480, 1012
Hsp92II CATG 2 cut(s) 563, 640
HspAI GCGC 3 cut(s) 142, 205, 1110
Kzo9I GATC 6 cut(s) 68, 112, 240, 261, 586, 949
LmnI GCTCC 5 cut(s) 314, 361, 836, 868, 876
Lsp1109I GCAGC 5 cut(s) 42, 201, 441, 658, 1099
LweI GCATC 5 cut(s) 14, 201, 494, 790, 814
MaeI CTAG 1 cut(s) 641
MaeII ACGT 5 cut(s) 177, 333, 480, 595, 909
MaeIII GTNAC 1 cut(s) 481
MalI GATC 6 cut(s) 70, 114, 242, 263, 588, 951
MboI GATC 6 cut(s) 68, 112, 240, 261, 586, 949
MboII GAAGA 4 cut(s) 116, 205, 1091, 1231
MflI RGATCY 4 cut(s) 68, 240, 586, 949
MhlI GDGCHC 5 cut(s) 311, 323, 350, 366, 873
MluCI AATT 4 cut(s) 325, 698, 992, 1241
MlyI GAGTC 2 cut(s) 101, 434
MmeI TCCRAC 3 cut(s) 96, 123, 447
MroXI GAANNNNTTC 2 cut(s) 152, 702
MseI TTAA 2 cut(s) 354, 912
MslI CAYNNNNRTG 1 cut(s) 405
MspA1I CMGCKG 1 cut(s) 256
MspI CCGG 6 cut(s) 47, 77, 358, 431, 969, 1143
MspR9I CCNGG 4 cut(s) 77, 359, 621, 969
Mva1269I GAATGC 2 cut(s) 772, 1152
MvaI CCWGG 1 cut(s) 621
MvnI CGCG 1 cut(s) 207
MwoI GCNNNNNNNGC 6 cut(s) 211, 220, 306, 370, 386, 1009
NciI CCSGG 3 cut(s) 77, 359, 969
NdeII GATC 6 cut(s) 68, 112, 240, 261, 586, 949
NheI GCTAGC 1 cut(s) 640
NlaIII CATG 2 cut(s) 563, 640
NlaIV GGNNCC 6 cut(s) 24, 242, 252, 310, 726, 951
NmeAIII GCCGAG 1 cut(s) 1072
NmuCI GTSAC 1 cut(s) 481
PaeR7I CTCGAG 2 cut(s) 275, 442
PaqCI CACCTGC 1 cut(s) 781
PcsI WCGNNNNNNNCGW 6 cut(s) 108, 156, 183, 282, 736, 1165
PctI GAATGC 2 cut(s) 772, 1152
PdmI GAANNNNTTC 2 cut(s) 152, 702
PfeI GAWTC 2 cut(s) 340, 858
PfoI TCCNGGA 1 cut(s) 967
PkrI GCNGC 8 cut(s) 57, 125, 216, 255, 429, 456, 673, 1114
PleI GAGTC 2 cut(s) 101, 434
PmaCI CACGTG 1 cut(s) 596
PmlI CACGTG 1 cut(s) 596
PpsI GAGTC 2 cut(s) 101, 434
Ppu21I YACGTR 2 cut(s) 334, 596
Psp124BI GAGCTC 1 cut(s) 873
Psp1406I AACGTT 1 cut(s) 909
Psp6I CCWGG 1 cut(s) 619
PspCI CACGTG 1 cut(s) 596
PspGI CCWGG 1 cut(s) 619
PspN4I GGNNCC 6 cut(s) 24, 242, 252, 310, 726, 951
PspPI GGNCC 4 cut(s) 22, 133, 614, 725
PspXI VCTCGAGB 1 cut(s) 442
PsuI RGATCY 4 cut(s) 68, 240, 586, 949
RsaI GTAC 1 cut(s) 176
RsaNI GTAC 1 cut(s) 175
RseI CAYNNNNRTG 1 cut(s) 405
SacI GAGCTC 1 cut(s) 873
SalI GTCGAC 1 cut(s) 284
SaqAI TTAA 2 cut(s) 354, 912
SatI GCNGC 8 cut(s) 56, 124, 215, 254, 428, 455, 672, 1113
Sau3AI GATC 6 cut(s) 68, 112, 240, 261, 586, 949
Sau96I GGNCC 4 cut(s) 22, 133, 614, 725
SchI GAGTC 2 cut(s) 101, 434
ScrFI CCNGG 4 cut(s) 77, 359, 621, 969
SduI GDGCHC 5 cut(s) 311, 323, 350, 366, 873
SfaNI GCATC 5 cut(s) 14, 201, 494, 790, 814
SfcI CTRYAG 2 cut(s) 118, 487
Sfr274I CTCGAG 2 cut(s) 275, 442
SinI GGWCC 2 cut(s) 22, 725
SlaI CTCGAG 2 cut(s) 275, 442
SmiMI CAYNNNNRTG 1 cut(s) 405
SmlI CTYRAG 4 cut(s) 128, 275, 442, 1057
SmoI CTYRAG 4 cut(s) 128, 275, 442, 1057
Sse9I AATT 4 cut(s) 325, 698, 992, 1241
SsiI CCGC 8 cut(s) 124, 217, 238, 254, 397, 428, 437, 751
SspMI CTAG 1 cut(s) 641
SstI GAGCTC 1 cut(s) 873
StyD4I CCNGG 4 cut(s) 75, 357, 619, 967
StyI CCWWGG 1 cut(s) 759
TaaI ACNGT 3 cut(s) 491, 904, 983
TaiI ACGT 5 cut(s) 180, 336, 483, 598, 912
TaqI TCGA 5 cut(s) 159, 276, 285, 323, 443
TasI AATT 4 cut(s) 325, 698, 992, 1241
TauI GCSGC 3 cut(s) 126, 256, 430
TfiI GAWTC 2 cut(s) 340, 858
Tru1I TTAA 2 cut(s) 354, 912
Tru9I TTAA 2 cut(s) 354, 912
TscAI CASTG 3 cut(s) 496, 543, 1048
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 5 cut(s) 55, 214, 454, 671, 1112
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 3 cut(s) 145, 667, 1067
TspGWI ACGGA 1 cut(s) 518
TspRI CASTG 3 cut(s) 496, 543, 1048
VpaK11BI GGWCC 2 cut(s) 22, 725
XagI CCTNNNNNAGG 1 cut(s) 758
XapI RAATTY 3 cut(s) 698, 992, 1241
XcmI CCANNNNNNNNNTGG 1 cut(s) 412
XhoI CTCGAG 2 cut(s) 275, 442
XmiI GTMKAC 2 cut(s) 285, 1071
XmnI GAANNNNTTC 2 cut(s) 152, 702
XspI CTAG 1 cut(s) 641
ZraI GACGTC 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.