Rh5AG235600

26S protease regulatory subunit 6B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002106
Physical Location & Seq
Reverse (-)
28626903 .. 28629233
2331 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG235600.1

Sequence Viewer

Length: 849 bp
ATGCAGCCTACTATTTCTTCCGACCTAAAGGTCACCGACCAGCACCAATCCTCCGACGAGGACGACCTCTATAGCCGCCTCAAGTCCCTGCAATGCCAGCTGGAGTTCATCGACATCCAAGAGGAGTACGTCAGGATTCTCAGCACTATTAATCGGGAGCATCTGAAGCCCTCCGCCTCTGTCGCCTTGCATCGCCACTCCAACGCCCTCGTTGACGTTCTGCCTCCGGAGGTCGACTCCAGTATTTCGCTGCTCAGCCAGAGCGAGAAGCCCGACGTCACCTATAACGATATCGGGGGATGCGACATTCAAAAGCAAGAAATCCGTGAAGCAGTGGAACTTCCACTTACACACCATGACTTATACAAACAGATTGGAATAGATCCTCCACGTGGTGTATTGCTGTATGGCCCACCTGGAACTGGTAAAACCATGCTAGCTAAGGTTGTTGCCAATCATACAACTGCTGCCTTCATTAGAGTTGTTGGTTCAGAATTTGTTCAGAAGTATTTGGGTGAGGGTCCACGAATGGTCTGTGATGTTTTCCGTCTCGCCAAGGAGAATGCACCTGCCATCATCTTTATTGATGAGGTGGATGCTATTGCTACTACAAGGTTTGATGCTCAAACTGGAGCTGATAGAGAAGTTCAGCGAATTCTTATGGAGCTCCTCAATCAGGTAGTTCCAGGAAGATTTTATATTGAAGAACAGAAATATTTTTGCGAAAGAAAGAATAAACATTTTGATTGTTTAAATATGCAAATATATTTGAACTGGGACATGCAAATGCATCGTTTGGAGTATATATATTCTCTGAAGAAGCAAGCACCAAAGGGTTCCTTTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005911 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0030054 GO:0030162 GO:0030163 GO:0030312 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055044 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

282

Amino Acids

32.22

Weight (kDa)

5.51

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 133 - 226 1.4e-28 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 577
AasI GACNNNNNNGTC 1 cut(s) 29
AatII GACGTC 1 cut(s) 279
Acc36I ACCTGC 1 cut(s) 577
AccI GTMKAC 1 cut(s) 234
AccIII TCCGGA 1 cut(s) 226
AciI CCGC 2 cut(s) 76, 174
AclWI GGATC 1 cut(s) 377
AcsI RAATTY 2 cut(s) 494, 654
AcuI CTGAAG 2 cut(s) 185, 836
AcvI CACGTG 1 cut(s) 392
AcyI GRCGYC 1 cut(s) 276
AdeI CACNNNGTG 1 cut(s) 395
AfaI GTAC 1 cut(s) 128
AfiI CCNNNNNNNGG 3 cut(s) 392, 422, 676
AgsI TTSAA 3 cut(s) 311, 704, 772
AjnI CCWGG 2 cut(s) 415, 685
AluBI AGCT 4 cut(s) 100, 440, 635, 667
AluI AGCT 4 cut(s) 100, 440, 635, 667
Alw21I GWGCWC 1 cut(s) 669
Alw26I GTCTC 1 cut(s) 554
AlwI GGATC 1 cut(s) 377
Aor13HI TCCGGA 1 cut(s) 226
AoxI GGCC 1 cut(s) 409
ApeKI GCWGC 3 cut(s) 4, 250, 467
ApoI RAATTY 2 cut(s) 494, 654
AseI ATTAAT 1 cut(s) 150
Asp700I GAANNNNTTC 1 cut(s) 498
AspS9I GGNCC 2 cut(s) 410, 521
AsuHPI GGTGA 3 cut(s) 25, 271, 527
AsuNHI GCTAGC 1 cut(s) 436
AvaII GGWCC 1 cut(s) 521
BanII GRGCYC 1 cut(s) 669
BbrPI CACGTG 1 cut(s) 392
Bbv12I GWGCWC 1 cut(s) 669
BbvI GCAGC 3 cut(s) 16, 237, 454
BccI CCATC 1 cut(s) 581
BcgI CGANNNNNNTGC 2 cut(s) 773, 807
BciT130I CCWGG 2 cut(s) 417, 687
BcoDI GTCTC 1 cut(s) 554
BfaI CTAG 1 cut(s) 437
BfmI CTRYAG 1 cut(s) 70
BfuAI ACCTGC 1 cut(s) 577
BisI GCNGC 4 cut(s) 5, 76, 251, 468
BlpI GCTNAGC 1 cut(s) 254
BlsI GCNGC 4 cut(s) 6, 77, 252, 469
Bme1390I CCNGG 2 cut(s) 417, 687
Bme18I GGWCC 1 cut(s) 521
BmgT120I GGNCC 2 cut(s) 410, 521
BmiI GGNNCC 2 cut(s) 522, 838
BmrFI CCNGG 2 cut(s) 417, 687
BmrI ACTGGG 1 cut(s) 784
BmsI GCATC 6 cut(s) 169, 199, 290, 586, 610, 799
BmtI GCTAGC 1 cut(s) 440
BmuI ACTGGG 1 cut(s) 784
BplI GAGNNNNNCTC 2 cut(s) 221, 253
BpmI CTGGAG 3 cut(s) 122, 223, 651
Bpu10I CCTNAGC 1 cut(s) 441
Bpu1102I GCTNAGC 1 cut(s) 254
BpuEI CTTGAG 1 cut(s) 65
BsaAI YACGTR 1 cut(s) 392
BsaHI GRCGYC 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 555
BsaWI WCCGGW 1 cut(s) 226
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bsc4I CCNNNNNNNGG 3 cut(s) 392, 422, 676
Bse1I ACTGG 4 cut(s) 240, 427, 634, 779
Bse3DI GCAATG 1 cut(s) 98
BseAI TCCGGA 1 cut(s) 226
BseBI CCWGG 2 cut(s) 417, 687
BseDI CCNNGG 1 cut(s) 555
BseGI GGATG 3 cut(s) 114, 305, 601
BseLI CCNNNNNNNGG 3 cut(s) 392, 422, 676
BseMI GCAATG 1 cut(s) 98
BseMII CTCAG 2 cut(s) 154, 268
BseNI ACTGG 4 cut(s) 240, 427, 634, 779
BseRI GAGGAG 2 cut(s) 137, 659
BseXI GCAGC 3 cut(s) 16, 237, 454
BshFI GGCC 1 cut(s) 411
BsiHKAI GWGCWC 1 cut(s) 669
BsiSI CCGG 1 cut(s) 227
BslFI GGGAC 2 cut(s) 70, 791
BslI CCNNNNNNNGG 3 cut(s) 392, 422, 676
BsmAI GTCTC 1 cut(s) 554
BsmBI CGTCTC 1 cut(s) 554
BsmFI GGGAC 2 cut(s) 70, 791
BsmI GAATGC 1 cut(s) 568
BsnI GGCC 1 cut(s) 411
Bsp1286I GDGCHC 1 cut(s) 669
Bsp13I TCCGGA 1 cut(s) 226
Bsp143I GATC 1 cut(s) 382
Bsp1720I GCTNAGC 1 cut(s) 254
BspACI CCGC 2 cut(s) 76, 174
BspANI GGCC 1 cut(s) 411
BspCNI CTCAG 2 cut(s) 153, 267
BspEI TCCGGA 1 cut(s) 226
BspLI GGNNCC 2 cut(s) 522, 838
BspMI ACCTGC 1 cut(s) 577
BspOI GCTAGC 1 cut(s) 440
BspPI GGATC 1 cut(s) 377
BsrDI GCAATG 1 cut(s) 98
BsrI ACTGG 4 cut(s) 240, 427, 634, 779
BssECI CCNNGG 1 cut(s) 555
BssMI GATC 1 cut(s) 382
BssNI GRCGYC 1 cut(s) 276
BssT1I CCWWGG 1 cut(s) 555
Bst2UI CCWGG 2 cut(s) 417, 687
BstACI GRCGYC 1 cut(s) 276
BstBAI YACGTR 1 cut(s) 392
BstC8I GCNNGC 3 cut(s) 98, 438, 825
BstDEI CTNAG 3 cut(s) 140, 254, 441
BstEII GGTNACC 1 cut(s) 31
BstENI CCTNNNNNAGG 1 cut(s) 674
BstF5I GGATG 3 cut(s) 114, 305, 601
BstKTI GATC 1 cut(s) 385
BstMAI GTCTC 1 cut(s) 554
BstMBI GATC 1 cut(s) 382
BstMWI GCNNNNNNNGC 3 cut(s) 97, 166, 182
BstNI CCWGG 2 cut(s) 417, 687
BstNSI RCATGY 1 cut(s) 784
BstPI GGTNACC 1 cut(s) 31
BstSCI CCNGG 2 cut(s) 415, 685
BstSFI CTRYAG 1 cut(s) 70
BstV1I GCAGC 3 cut(s) 16, 237, 454
BstX2I RGATCY 1 cut(s) 382
BstYI RGATCY 1 cut(s) 382
BsuRI GGCC 1 cut(s) 411
BtgZI GCGATG 1 cut(s) 176
BtsCI GGATG 3 cut(s) 114, 305, 601
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 1 cut(s) 339
BveI ACCTGC 1 cut(s) 577
Cac8I GCNNGC 3 cut(s) 98, 438, 825
Cfr13I GGNCC 2 cut(s) 410, 521
Csp6I GTAC 1 cut(s) 127
CviAII CATG 3 cut(s) 356, 433, 781
CviQI GTAC 1 cut(s) 127
DdeI CTNAG 3 cut(s) 140, 254, 441
DpnI GATC 1 cut(s) 384
DpnII GATC 1 cut(s) 382
DraI TTTAAA 1 cut(s) 753
DraIII CACNNNGTG 1 cut(s) 395
DrdI GACNNNNNNGTC 1 cut(s) 29
DseDI GACNNNNNNGTC 1 cut(s) 29
EciI GGCGGA 1 cut(s) 163
Ecl136II GAGCTC 1 cut(s) 667
Eco130I CCWWGG 1 cut(s) 555
Eco24I GRGCYC 1 cut(s) 669
Eco32I GATATC 1 cut(s) 292
Eco47I GGWCC 1 cut(s) 521
Eco53kI GAGCTC 1 cut(s) 667
Eco57I CTGAAG 2 cut(s) 185, 836
Eco72I CACGTG 1 cut(s) 392
Eco91I GGTNACC 1 cut(s) 31
EcoICRI GAGCTC 1 cut(s) 667
EcoNI CCTNNNNNAGG 1 cut(s) 674
EcoO65I GGTNACC 1 cut(s) 31
EcoRI GAATTC 1 cut(s) 654
EcoRII CCWGG 2 cut(s) 415, 685
EcoRV GATATC 1 cut(s) 292
EcoT14I CCWWGG 1 cut(s) 555
EcoT22I ATGCAT 1 cut(s) 792
EcoT38I GRGCYC 1 cut(s) 669
ErhI CCWWGG 1 cut(s) 555
Esp3I CGTCTC 1 cut(s) 554
FaeI CATG 3 cut(s) 359, 436, 784
FalI AAGNNNNNCTT 1 cut(s) 824
FaqI GGGAC 2 cut(s) 70, 791
FatI CATG 3 cut(s) 355, 432, 780
FblI GTMKAC 1 cut(s) 234
Fnu4HI GCNGC 4 cut(s) 5, 76, 251, 468
FokI GGATG 3 cut(s) 101, 312, 608
FriOI GRGCYC 1 cut(s) 669
Fsp4HI GCNGC 4 cut(s) 5, 76, 251, 468
FspBI CTAG 1 cut(s) 437
GluI GCNGC 4 cut(s) 5, 76, 251, 468
GsuI CTGGAG 3 cut(s) 122, 223, 651
HaeIII GGCC 1 cut(s) 411
HapII CCGG 1 cut(s) 227
Hin1I GRCGYC 1 cut(s) 276
Hin1II CATG 3 cut(s) 359, 436, 784
HincII GTYRAC 2 cut(s) 214, 235
HindII GTYRAC 2 cut(s) 214, 235
HinfI GANTC 2 cut(s) 136, 236
HpaII CCGG 1 cut(s) 227
HphI GGTGA 3 cut(s) 25, 271, 527
Hpy166II GTNNAC 3 cut(s) 214, 235, 524
Hpy188I TCNGA 6 cut(s) 22, 55, 165, 493, 504, 816
Hpy188III TCNNGA 3 cut(s) 133, 155, 227
Hpy8I GTNNAC 3 cut(s) 214, 235, 524
Hpy99I CGWCG 2 cut(s) 59, 278
HpyAV CCTTC 1 cut(s) 481
HpyCH4IV ACGT 4 cut(s) 129, 216, 276, 391
HpyCH4V TGCA 7 cut(s) 4, 91, 190, 566, 760, 784, 790
HpyF10VI GCNNNNNNNGC 3 cut(s) 97, 166, 182
HpyF3I CTNAG 3 cut(s) 140, 254, 441
HpySE526I ACGT 4 cut(s) 129, 216, 276, 391
Hsp92I GRCGYC 1 cut(s) 276
Hsp92II CATG 3 cut(s) 359, 436, 784
Kpn2I TCCGGA 1 cut(s) 226
Kzo9I GATC 1 cut(s) 382
LmnI GCTCC 4 cut(s) 157, 632, 664, 672
Lsp1109I GCAGC 3 cut(s) 16, 237, 454
LweI GCATC 6 cut(s) 169, 199, 290, 586, 610, 799
MaeI CTAG 1 cut(s) 437
MaeII ACGT 4 cut(s) 129, 216, 276, 391
MaeIII GTNAC 2 cut(s) 31, 277
MalI GATC 1 cut(s) 384
MboI GATC 1 cut(s) 382
MboII GAAGA 4 cut(s) 9, 702, 716, 829
MflI RGATCY 1 cut(s) 382
MhlI GDGCHC 1 cut(s) 669
MluCI AATT 2 cut(s) 494, 654
MlyI GAGTC 1 cut(s) 230
MmeI TCCRAC 3 cut(s) 45, 78, 225
Mph1103I ATGCAT 1 cut(s) 792
MroI TCCGGA 1 cut(s) 226
MroXI GAANNNNTTC 1 cut(s) 498
MseI TTAA 2 cut(s) 150, 752
MslI CAYNNNNRTG 1 cut(s) 785
MspA1I CMGCKG 1 cut(s) 100
MspI CCGG 1 cut(s) 227
MspR9I CCNGG 2 cut(s) 417, 687
Mva1269I GAATGC 1 cut(s) 568
MvaI CCWGG 2 cut(s) 417, 687
MwoI GCNNNNNNNGC 3 cut(s) 97, 166, 182
NdeII GATC 1 cut(s) 382
NheI GCTAGC 1 cut(s) 436
NlaIII CATG 3 cut(s) 359, 436, 784
NlaIV GGNNCC 2 cut(s) 522, 838
NmuCI GTSAC 2 cut(s) 31, 277
NsiI ATGCAT 1 cut(s) 792
NspI RCATGY 1 cut(s) 784
PaqCI CACCTGC 1 cut(s) 577
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PctI GAATGC 1 cut(s) 568
PdmI GAANNNNTTC 1 cut(s) 498
PfeI GAWTC 1 cut(s) 136
PfoI TCCNGGA 1 cut(s) 685
PkrI GCNGC 4 cut(s) 6, 77, 252, 469
PleI GAGTC 1 cut(s) 230
PmaCI CACGTG 1 cut(s) 392
PmlI CACGTG 1 cut(s) 392
PpsI GAGTC 1 cut(s) 230
Ppu21I YACGTR 1 cut(s) 392
PshBI ATTAAT 1 cut(s) 150
Psp124BI GAGCTC 1 cut(s) 669
Psp6I CCWGG 2 cut(s) 415, 685
PspCI CACGTG 1 cut(s) 392
PspEI GGTNACC 1 cut(s) 31
PspGI CCWGG 2 cut(s) 415, 685
PspN4I GGNNCC 2 cut(s) 522, 838
PspPI GGNCC 2 cut(s) 410, 521
PsuI RGATCY 1 cut(s) 382
PvuII CAGCTG 1 cut(s) 100
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
RseI CAYNNNNRTG 1 cut(s) 785
SacI GAGCTC 1 cut(s) 669
SalI GTCGAC 1 cut(s) 233
SaqAI TTAA 2 cut(s) 150, 752
SatI GCNGC 4 cut(s) 5, 76, 251, 468
Sau3AI GATC 1 cut(s) 382
Sau96I GGNCC 2 cut(s) 410, 521
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 2 cut(s) 417, 687
SduI GDGCHC 1 cut(s) 669
SfaNI GCATC 6 cut(s) 169, 199, 290, 586, 610, 799
SfcI CTRYAG 1 cut(s) 70
SinI GGWCC 1 cut(s) 521
SmiMI CAYNNNNRTG 1 cut(s) 785
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
Sse9I AATT 2 cut(s) 494, 654
SsiI CCGC 2 cut(s) 76, 174
SspI AATATT 1 cut(s) 716
SspMI CTAG 1 cut(s) 437
SstI GAGCTC 1 cut(s) 669
StyD4I CCNGG 2 cut(s) 415, 685
StyI CCWWGG 1 cut(s) 555
TaiI ACGT 4 cut(s) 132, 219, 279, 394
TaqI TCGA 2 cut(s) 111, 234
TasI AATT 2 cut(s) 494, 654
TauI GCSGC 1 cut(s) 78
TfiI GAWTC 1 cut(s) 136
Tru1I TTAA 2 cut(s) 150, 752
Tru9I TTAA 2 cut(s) 150, 752
TscAI CASTG 1 cut(s) 339
TseFI GTSAC 2 cut(s) 31, 277
TseI GCWGC 3 cut(s) 4, 250, 467
Tsp45I GTSAC 2 cut(s) 31, 277
TspDTI ATGAA 2 cut(s) 97, 463
TspGWI ACGGA 2 cut(s) 314, 536
TspRI CASTG 1 cut(s) 339
VpaK11BI GGWCC 1 cut(s) 521
VspI ATTAAT 1 cut(s) 150
XagI CCTNNNNNAGG 1 cut(s) 674
XapI RAATTY 2 cut(s) 494, 654
XceI RCATGY 1 cut(s) 784
XmiI GTMKAC 1 cut(s) 234
XmnI GAANNNNTTC 1 cut(s) 498
XspI CTAG 1 cut(s) 437
ZraI GACGTC 1 cut(s) 277
Zsp2I ATGCAT 1 cut(s) 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.