Rh1BG054900

26S protease regulatory subunit 6B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0002106
Physical Location & Seq
Forward (+)
8031732 .. 8034030
2299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG054900.1

Sequence Viewer

Length: 705 bp
ATGGCAGCGTCCGCGATGGTGTTGGACCCAAAACCCGCGGCGGAGCCACCCGCTTCGTTCCCATCCACAAGATCCGACCTAAAGCTCAGCGACCAGCACCAATCCTCCGACGAGGACGACCTCTACAGCCGCCTCAAGTCCCTGCAGCGCCAGCTGGAGTTCATCGACATCCAAGAGGAGTACGTCAAAGACGAGCAGAAGAATCTAAAGCGCGAGCTCCTCCGCGCCCAGGAGGAGGTGAAGAGGATCCAATCGGTGCCTCTGGTGATCGGACAGTTCATGGAGATGGTGGATCAGAACAACGGCATCGTCGGGTCCACCACCGGCTCGAATTACTATGTCAGGATTCTCAGCACTATTAACCGAGAGCATCTGAAGCCCTCCGCCTCCGTCGCGTTGCACCGCCACTCCAACGCCCTCGTCGACGTTCTGCCCCCGGAGGCCGACTCCAGTATTTCGCTGCTCAGCCAGAGCGAGAAGCCCGACGTCACCTATAACGGTCCACGAATGGTCCGTGATGTTTTCCGTCTCGCCAAGGAGAATGCACCTGCCATCATCTTTATTGATGAGGTGGATGCTATTGCTACTGCAAGGTTTGATGCTCAAACTGGAGCTGATAGAGAAGTTCAGCGAATTCTTATGGAGCTCCTCAATCAGGTAGTTCCAGGAAGATTTTATCTTGAAGAACAGAAATATTTCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005911 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0030054 GO:0030162 GO:0030163 GO:0030312 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055044 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

234

Amino Acids

26.45

Weight (kDa)

5.05

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_ATP_ID_OB_C PF16450 90 - 145 6.1e-12 Proteasomal ATPase OB C-terminal domain
AAA PF00004 163 - 219 2.9e-10 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 556
AatII GACGTC 1 cut(s) 489
Acc36I ACCTGC 1 cut(s) 556
AccB1I GGYRCC 1 cut(s) 256
AccI GTMKAC 1 cut(s) 423
AccII CGCG 5 cut(s) 14, 38, 213, 225, 395
AciI CCGC 9 cut(s) 12, 36, 38, 41, 51, 130, 223, 384, 403
AclWI GGATC 4 cut(s) 66, 241, 254, 300
AcsI RAATTY 1 cut(s) 633
AcuI CTGAAG 1 cut(s) 395
AcyI GRCGYC 1 cut(s) 486
AfaI GTAC 1 cut(s) 182
AfiI CCNNNNNNNGG 3 cut(s) 229, 235, 655
AgsI TTSAA 1 cut(s) 683
AjnI CCWGG 2 cut(s) 228, 664
AluBI AGCT 5 cut(s) 85, 154, 217, 614, 646
AluI AGCT 5 cut(s) 85, 154, 217, 614, 646
Alw21I GWGCWC 2 cut(s) 219, 648
Alw26I GTCTC 1 cut(s) 533
AlwI GGATC 4 cut(s) 66, 241, 254, 300
AoxI GGCC 1 cut(s) 441
ApeKI GCWGC 3 cut(s) 5, 145, 460
ApoI RAATTY 1 cut(s) 633
Asp700I GAANNNNTTC 1 cut(s) 695
AspLEI GCGC 3 cut(s) 150, 213, 227
AspS9I GGNCC 4 cut(s) 25, 315, 500, 511
AsuC2I CCSGG 1 cut(s) 437
AsuHPI GGTGA 3 cut(s) 250, 277, 481
AvaII GGWCC 4 cut(s) 25, 315, 500, 511
BamHI GGATCC 1 cut(s) 246
BanI GGYRCC 1 cut(s) 256
BanII GRGCYC 2 cut(s) 219, 648
Bbv12I GWGCWC 2 cut(s) 219, 648
BbvI GCAGC 3 cut(s) 17, 157, 447
BccI CCATC 4 cut(s) 10, 70, 280, 560
BceAI ACGGC 1 cut(s) 319
BciT130I CCWGG 2 cut(s) 230, 666
BcnI CCSGG 1 cut(s) 437
BcoDI GTCTC 1 cut(s) 533
BfmI CTRYAG 2 cut(s) 124, 143
BfoI RGCGCY 1 cut(s) 151
BfuAI ACCTGC 1 cut(s) 556
BisI GCNGC 5 cut(s) 6, 39, 130, 146, 461
BlpI GCTNAGC 2 cut(s) 86, 464
BlsI GCNGC 5 cut(s) 7, 40, 131, 147, 462
Bme1390I CCNGG 3 cut(s) 230, 437, 666
Bme18I GGWCC 4 cut(s) 25, 315, 500, 511
BmgT120I GGNCC 4 cut(s) 25, 315, 500, 511
BmiI GGNNCC 5 cut(s) 27, 45, 248, 258, 316
BmrFI CCNGG 3 cut(s) 230, 437, 666
BmsI GCATC 4 cut(s) 315, 379, 565, 589
BplI GAGNNNNNCTC 2 cut(s) 431, 463
BpmI CTGGAG 3 cut(s) 176, 433, 630
Bpu1102I GCTNAGC 2 cut(s) 86, 464
BpuEI CTTGAG 1 cut(s) 119
BpuMI CCSGG 1 cut(s) 437
BsaHI GRCGYC 1 cut(s) 486
BsaJI CCNNGG 4 cut(s) 36, 228, 435, 534
BsaXI ACNNNNNCTCC 4 cut(s) 89, 119, 392, 422
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 235, 655
Bse118I RCCGGY 1 cut(s) 323
Bse1I ACTGG 2 cut(s) 450, 613
BseBI CCWGG 2 cut(s) 230, 666
BseDI CCNNGG 4 cut(s) 36, 228, 435, 534
BseGI GGATG 3 cut(s) 62, 168, 580
BseLI CCNNNNNNNGG 3 cut(s) 229, 235, 655
BseMII CTCAG 3 cut(s) 100, 364, 478
BseNI ACTGG 2 cut(s) 450, 613
BseRI GAGGAG 4 cut(s) 191, 209, 248, 638
BseXI GCAGC 3 cut(s) 17, 157, 447
Bsh1236I CGCG 5 cut(s) 14, 38, 213, 225, 395
BshFI GGCC 1 cut(s) 443
BshNI GGYRCC 1 cut(s) 256
BsiHKAI GWGCWC 2 cut(s) 219, 648
BsiSI CCGG 2 cut(s) 324, 437
BslFI GGGAC 1 cut(s) 124
BslI CCNNNNNNNGG 3 cut(s) 229, 235, 655
BsmAI GTCTC 1 cut(s) 533
BsmBI CGTCTC 1 cut(s) 533
BsmFI GGGAC 1 cut(s) 124
BsmI GAATGC 1 cut(s) 547
BsnI GGCC 1 cut(s) 443
Bsp1286I GDGCHC 2 cut(s) 219, 648
Bsp143I GATC 4 cut(s) 71, 246, 267, 292
Bsp1720I GCTNAGC 2 cut(s) 86, 464
BspACI CCGC 9 cut(s) 12, 36, 38, 41, 51, 130, 223, 384, 403
BspANI GGCC 1 cut(s) 443
BspCNI CTCAG 3 cut(s) 99, 363, 477
BspFNI CGCG 5 cut(s) 14, 38, 213, 225, 395
BspLI GGNNCC 5 cut(s) 27, 45, 248, 258, 316
BspMAI CTGCAG 1 cut(s) 147
BspMI ACCTGC 1 cut(s) 556
BspPI GGATC 4 cut(s) 66, 241, 254, 300
BspT107I GGYRCC 1 cut(s) 256
BsrFI RCCGGY 1 cut(s) 323
BsrI ACTGG 2 cut(s) 450, 613
BssAI RCCGGY 1 cut(s) 323
BssECI CCNNGG 4 cut(s) 36, 228, 435, 534
BssMI GATC 4 cut(s) 71, 246, 267, 292
BssNI GRCGYC 1 cut(s) 486
BssT1I CCWWGG 1 cut(s) 534
Bst2UI CCWGG 2 cut(s) 230, 666
Bst4CI ACNGT 2 cut(s) 276, 500
Bst6I CTCTTC 1 cut(s) 236
BstACI GRCGYC 1 cut(s) 486
BstC8I GCNNGC 2 cut(s) 152, 215
BstDEI CTNAG 3 cut(s) 86, 350, 464
BstDSI CCRYGG 1 cut(s) 36
BstENI CCTNNNNNAGG 1 cut(s) 653
BstF5I GGATG 3 cut(s) 62, 168, 580
BstFNI CGCG 5 cut(s) 14, 38, 213, 225, 395
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 3 cut(s) 150, 213, 227
BstKTI GATC 4 cut(s) 74, 249, 270, 295
BstMAI GTCTC 1 cut(s) 533
BstMBI GATC 4 cut(s) 71, 246, 267, 292
BstMWI GCNNNNNNNGC 4 cut(s) 11, 151, 376, 392
BstNI CCWGG 2 cut(s) 230, 666
BstSCI CCNGG 3 cut(s) 228, 435, 664
BstSFI CTRYAG 2 cut(s) 124, 143
BstUI CGCG 5 cut(s) 14, 38, 213, 225, 395
BstV1I GCAGC 3 cut(s) 17, 157, 447
BstX2I RGATCY 2 cut(s) 71, 246
BstYI RGATCY 2 cut(s) 71, 246
BsuRI GGCC 1 cut(s) 443
BtgI CCRYGG 1 cut(s) 36
BtgZI GCGATG 1 cut(s) 29
BtsCI GGATG 3 cut(s) 62, 168, 580
BveI ACCTGC 1 cut(s) 556
Cac8I GCNNGC 2 cut(s) 152, 215
CfoI GCGC 3 cut(s) 150, 213, 227
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 4 cut(s) 25, 315, 500, 511
Cfr42I CCGCGG 1 cut(s) 39
Csp6I GTAC 1 cut(s) 181
CviAII CATG 1 cut(s) 280
CviQI GTAC 1 cut(s) 181
DdeI CTNAG 3 cut(s) 86, 350, 464
DpnI GATC 4 cut(s) 73, 248, 269, 294
DpnII GATC 4 cut(s) 71, 246, 267, 292
Eam1104I CTCTTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 236
EciI GGCGGA 2 cut(s) 56, 373
Ecl136II GAGCTC 2 cut(s) 217, 646
Eco130I CCWWGG 1 cut(s) 534
Eco24I GRGCYC 2 cut(s) 219, 648
Eco47I GGWCC 4 cut(s) 25, 315, 500, 511
Eco53kI GAGCTC 2 cut(s) 217, 646
Eco57I CTGAAG 1 cut(s) 395
EcoICRI GAGCTC 2 cut(s) 217, 646
EcoNI CCTNNNNNAGG 1 cut(s) 653
EcoRI GAATTC 1 cut(s) 633
EcoRII CCWGG 2 cut(s) 228, 664
EcoT14I CCWWGG 1 cut(s) 534
EcoT38I GRGCYC 2 cut(s) 219, 648
ErhI CCWWGG 1 cut(s) 534
Esp3I CGTCTC 1 cut(s) 533
FaeI CATG 1 cut(s) 283
FaiI YATR 4 cut(s) 281, 339, 495, 641
FaqI GGGAC 1 cut(s) 124
FatI CATG 1 cut(s) 279
FauI CCCGC 2 cut(s) 43, 58
FblI GTMKAC 1 cut(s) 423
Fnu4HI GCNGC 5 cut(s) 6, 39, 130, 146, 461
FokI GGATG 3 cut(s) 49, 155, 587
FriOI GRGCYC 2 cut(s) 219, 648
Fsp4HI GCNGC 5 cut(s) 6, 39, 130, 146, 461
GlaI GCGC 3 cut(s) 149, 212, 226
GluI GCNGC 5 cut(s) 6, 39, 130, 146, 461
GsuI CTGGAG 3 cut(s) 176, 433, 630
HaeII RGCGCY 1 cut(s) 151
HaeIII GGCC 1 cut(s) 443
HapII CCGG 2 cut(s) 324, 437
HhaI GCGC 3 cut(s) 150, 213, 227
Hin1I GRCGYC 1 cut(s) 486
Hin1II CATG 1 cut(s) 283
Hin6I GCGC 3 cut(s) 148, 211, 225
HinP1I GCGC 3 cut(s) 148, 211, 225
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HinfI GANTC 3 cut(s) 202, 346, 446
HpaII CCGG 2 cut(s) 324, 437
HphI GGTGA 3 cut(s) 250, 277, 481
Hpy166II GTNNAC 3 cut(s) 318, 424, 503
Hpy188I TCNGA 5 cut(s) 76, 109, 272, 297, 375
Hpy188III TCNNGA 2 cut(s) 343, 680
Hpy8I GTNNAC 3 cut(s) 318, 424, 503
Hpy99I CGWCG 6 cut(s) 113, 314, 395, 425, 428, 488
HpyCH4III ACNGT 2 cut(s) 276, 500
HpyCH4IV ACGT 3 cut(s) 183, 426, 486
HpyCH4V TGCA 4 cut(s) 145, 400, 545, 590
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 151, 376, 392
HpyF3I CTNAG 3 cut(s) 86, 350, 464
HpySE526I ACGT 3 cut(s) 183, 426, 486
Hsp92I GRCGYC 1 cut(s) 486
Hsp92II CATG 1 cut(s) 283
HspAI GCGC 3 cut(s) 148, 211, 225
KspI CCGCGG 1 cut(s) 39
Kzo9I GATC 4 cut(s) 71, 246, 267, 292
LmnI GCTCC 5 cut(s) 43, 222, 611, 643, 651
Lsp1109I GCAGC 3 cut(s) 17, 157, 447
LweI GCATC 4 cut(s) 315, 379, 565, 589
MaeII ACGT 3 cut(s) 183, 426, 486
MaeIII GTNAC 1 cut(s) 487
MalI GATC 4 cut(s) 73, 248, 269, 294
MboI GATC 4 cut(s) 71, 246, 267, 292
MboII GAAGA 4 cut(s) 211, 253, 681, 695
MflI RGATCY 2 cut(s) 71, 246
MhlI GDGCHC 2 cut(s) 219, 648
MluCI AATT 2 cut(s) 331, 633
MlyI GAGTC 1 cut(s) 440
MmeI TCCRAC 3 cut(s) 99, 132, 435
MroXI GAANNNNTTC 1 cut(s) 695
MseI TTAA 1 cut(s) 360
MslI CAYNNNNRTG 1 cut(s) 284
MspA1I CMGCKG 2 cut(s) 38, 154
MspI CCGG 2 cut(s) 324, 437
MspR9I CCNGG 3 cut(s) 230, 437, 666
Mva1269I GAATGC 1 cut(s) 547
MvaI CCWGG 2 cut(s) 230, 666
MvnI CGCG 5 cut(s) 14, 38, 213, 225, 395
MwoI GCNNNNNNNGC 4 cut(s) 11, 151, 376, 392
NciI CCSGG 1 cut(s) 437
NdeII GATC 4 cut(s) 71, 246, 267, 292
NlaIII CATG 1 cut(s) 283
NlaIV GGNNCC 5 cut(s) 27, 45, 248, 258, 316
NmuCI GTSAC 1 cut(s) 487
PaqCI CACCTGC 1 cut(s) 556
PcsI WCGNNNNNNNCGW 3 cut(s) 189, 420, 511
PctI GAATGC 1 cut(s) 547
PdmI GAANNNNTTC 1 cut(s) 695
PfeI GAWTC 2 cut(s) 202, 346
PfoI TCCNGGA 1 cut(s) 664
PkrI GCNGC 5 cut(s) 7, 40, 131, 147, 462
PleI GAGTC 1 cut(s) 440
PpsI GAGTC 1 cut(s) 440
Psp124BI GAGCTC 2 cut(s) 219, 648
Psp6I CCWGG 2 cut(s) 228, 664
PspGI CCWGG 2 cut(s) 228, 664
PspN4I GGNNCC 5 cut(s) 27, 45, 248, 258, 316
PspPI GGNCC 4 cut(s) 25, 315, 500, 511
PstI CTGCAG 1 cut(s) 147
PsuI RGATCY 2 cut(s) 71, 246
PvuII CAGCTG 1 cut(s) 154
RsaI GTAC 1 cut(s) 182
RsaNI GTAC 1 cut(s) 181
RseI CAYNNNNRTG 1 cut(s) 284
SacI GAGCTC 2 cut(s) 219, 648
SacII CCGCGG 1 cut(s) 39
SalI GTCGAC 1 cut(s) 422
SaqAI TTAA 1 cut(s) 360
SatI GCNGC 5 cut(s) 6, 39, 130, 146, 461
Sau3AI GATC 4 cut(s) 71, 246, 267, 292
Sau96I GGNCC 4 cut(s) 25, 315, 500, 511
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 3 cut(s) 230, 437, 666
SduI GDGCHC 2 cut(s) 219, 648
SfaNI GCATC 4 cut(s) 315, 379, 565, 589
SfcI CTRYAG 2 cut(s) 124, 143
Sfr303I CCGCGG 1 cut(s) 39
SgrBI CCGCGG 1 cut(s) 39
SgrDI CGTCGACG 1 cut(s) 422
SinI GGWCC 4 cut(s) 25, 315, 500, 511
SmiMI CAYNNNNRTG 1 cut(s) 284
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 2 cut(s) 331, 633
SsiI CCGC 9 cut(s) 12, 36, 38, 41, 51, 130, 223, 384, 403
SspI AATATT 1 cut(s) 695
SstI GAGCTC 2 cut(s) 219, 648
StyD4I CCNGG 3 cut(s) 228, 435, 664
StyI CCWWGG 1 cut(s) 534
TaaI ACNGT 2 cut(s) 276, 500
TaiI ACGT 3 cut(s) 186, 429, 489
TaqI TCGA 3 cut(s) 165, 329, 423
TasI AATT 2 cut(s) 331, 633
TauI GCSGC 2 cut(s) 41, 132
TfiI GAWTC 2 cut(s) 202, 346
Tru1I TTAA 1 cut(s) 360
Tru9I TTAA 1 cut(s) 360
TseFI GTSAC 1 cut(s) 487
TseI GCWGC 3 cut(s) 5, 145, 460
Tsp45I GTSAC 1 cut(s) 487
TspDTI ATGAA 2 cut(s) 151, 268
TspGWI ACGGA 3 cut(s) 379, 503, 515
VpaK11BI GGWCC 4 cut(s) 25, 315, 500, 511
XagI CCTNNNNNAGG 1 cut(s) 653
XapI RAATTY 1 cut(s) 633
XmiI GTMKAC 1 cut(s) 423
XmnI GAANNNNTTC 1 cut(s) 695
ZraI GACGTC 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.