Rw5G025060

26S protease regulatory subunit 6B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002106
Physical Location & Seq
Reverse (-)
35602580 .. 35604785
2206 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G025060.1

Sequence Viewer

Length: 438 bp
ATGTTGGTTGATATCGGGGGATGCGACATTCAAAAGCAAGAAATCCGTGAAGCAGTGGAACTTCCACTTACACACCATGACTTATACAAACAGATTGGAATAGATCCTCCACGTGGTGTATTGCTGTATGGCCCACCTGGAACTGGTAAAACCATGCTAGCTAAGGCTGTTGCCAATCATACAACTGCTGCCTTCATTAGAGTTGTTGGTTCAGAATTTGTTCAGAAGTATTTGGGTGGGGGTCCACGAATGGTCCATGATGTTTTCCGTCTCGCCAAGGAGAATGCACCTGCCATCATCTTTATTGATGAGGTGGATGCTATTGCTACTACAAGGTTTGATGCTCAAACTGGAGCTGATAGAAAAGTTCAGCAAATTCTTATGAAGCTCCTCAATCAGGTAAGCCTGCAAGAGAAGTTGCAGCTTTACGACTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005911 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0030054 GO:0030162 GO:0030163 GO:0030312 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055044 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

145

Amino Acids

16.06

Weight (kDa)

6.41

Isoelectric Point (pI)

25.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_16 PF13191 27 - 74 3.1e-06 AAA ATPase domain
AAA_5 PF07728 39 - 111 4.5e-06 AAA domain (dynein-related subfamily)
AAA PF00004 40 - 136 3.2e-30 ATPase family associated with various cellular activities (AAA)
AAA_2 PF07724 40 - 139 1.5e-07 AAA domain (Cdc48 subfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 298
Acc36I ACCTGC 1 cut(s) 298
AclWI GGATC 1 cut(s) 98
AcsI RAATTY 2 cut(s) 215, 375
AcvI CACGTG 1 cut(s) 113
AdeI CACNNNGTG 1 cut(s) 116
AfiI CCNNNNNNNGG 3 cut(s) 113, 143, 397
AgsI TTSAA 1 cut(s) 32
AjnI CCWGG 1 cut(s) 136
AluBI AGCT 4 cut(s) 161, 356, 388, 424
AluI AGCT 4 cut(s) 161, 356, 388, 424
Alw26I GTCTC 1 cut(s) 275
AlwI GGATC 1 cut(s) 98
AoxI GGCC 1 cut(s) 130
ApeKI GCWGC 2 cut(s) 188, 421
ApoI RAATTY 2 cut(s) 215, 375
Asp700I GAANNNNTTC 1 cut(s) 219
AspS9I GGNCC 3 cut(s) 131, 242, 253
AsuNHI GCTAGC 1 cut(s) 157
AvaII GGWCC 2 cut(s) 242, 253
BbrPI CACGTG 1 cut(s) 113
BbvI GCAGC 2 cut(s) 175, 433
BccI CCATC 1 cut(s) 302
BciT130I CCWGG 1 cut(s) 138
BcoDI GTCTC 1 cut(s) 275
BfaI CTAG 2 cut(s) 158, 436
BfuAI ACCTGC 1 cut(s) 298
BisI GCNGC 2 cut(s) 189, 422
BlsI GCNGC 2 cut(s) 190, 423
Bme1390I CCNGG 1 cut(s) 138
Bme18I GGWCC 2 cut(s) 242, 253
BmgT120I GGNCC 3 cut(s) 131, 242, 253
BmiI GGNNCC 1 cut(s) 243
BmrFI CCNGG 1 cut(s) 138
BmsI GCATC 3 cut(s) 11, 307, 331
BmtI GCTAGC 1 cut(s) 161
BpmI CTGGAG 1 cut(s) 372
Bpu10I CCTNAGC 1 cut(s) 162
BsaAI YACGTR 1 cut(s) 113
BsaJI CCNNGG 1 cut(s) 276
Bsc4I CCNNNNNNNGG 3 cut(s) 113, 143, 397
Bse1I ACTGG 2 cut(s) 148, 355
BseBI CCWGG 1 cut(s) 138
BseDI CCNNGG 1 cut(s) 276
BseGI GGATG 2 cut(s) 26, 322
BseLI CCNNNNNNNGG 3 cut(s) 113, 143, 397
BseNI ACTGG 2 cut(s) 148, 355
BseRI GAGGAG 1 cut(s) 380
BseXI GCAGC 2 cut(s) 175, 433
BshFI GGCC 1 cut(s) 132
BslI CCNNNNNNNGG 3 cut(s) 113, 143, 397
BsmAI GTCTC 1 cut(s) 275
BsmBI CGTCTC 1 cut(s) 275
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 1 cut(s) 132
Bsp143I GATC 1 cut(s) 103
BspANI GGCC 1 cut(s) 132
BspLI GGNNCC 1 cut(s) 243
BspMI ACCTGC 1 cut(s) 298
BspOI GCTAGC 1 cut(s) 161
BspPI GGATC 1 cut(s) 98
BsrI ACTGG 2 cut(s) 148, 355
BssECI CCNNGG 1 cut(s) 276
BssMI GATC 1 cut(s) 103
BssT1I CCWWGG 1 cut(s) 276
Bst2UI CCWGG 1 cut(s) 138
BstBAI YACGTR 1 cut(s) 113
BstC8I GCNNGC 2 cut(s) 159, 407
BstDEI CTNAG 1 cut(s) 162
BstENI CCTNNNNNAGG 1 cut(s) 395
BstF5I GGATG 2 cut(s) 26, 322
BstKTI GATC 1 cut(s) 106
BstMAI GTCTC 1 cut(s) 275
BstMBI GATC 1 cut(s) 103
BstNI CCWGG 1 cut(s) 138
BstSCI CCNGG 1 cut(s) 136
BstV1I GCAGC 2 cut(s) 175, 433
BstX2I RGATCY 1 cut(s) 103
BstYI RGATCY 1 cut(s) 103
BsuRI GGCC 1 cut(s) 132
BtsCI GGATG 2 cut(s) 26, 322
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 1 cut(s) 60
BveI ACCTGC 1 cut(s) 298
Cac8I GCNNGC 2 cut(s) 159, 407
Cfr13I GGNCC 3 cut(s) 131, 242, 253
CviAII CATG 3 cut(s) 77, 154, 257
CviJI RGCY 7 cut(s) 132, 161, 167, 356, 388, 405, 424
CviKI_1 RGCY 7 cut(s) 132, 161, 167, 356, 388, 405, 424
DdeI CTNAG 1 cut(s) 162
DpnI GATC 1 cut(s) 105
DpnII GATC 1 cut(s) 103
DraIII CACNNNGTG 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 276
Eco32I GATATC 1 cut(s) 13
Eco47I GGWCC 2 cut(s) 242, 253
Eco72I CACGTG 1 cut(s) 113
EcoNI CCTNNNNNAGG 1 cut(s) 395
EcoRII CCWGG 1 cut(s) 136
EcoRV GATATC 1 cut(s) 13
EcoT14I CCWWGG 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 276
Esp3I CGTCTC 1 cut(s) 275
FaeI CATG 3 cut(s) 80, 157, 260
FaiI YATR 7 cut(s) 78, 85, 129, 155, 180, 258, 383
FatI CATG 3 cut(s) 76, 153, 256
Fnu4HI GCNGC 2 cut(s) 189, 422
FokI GGATG 2 cut(s) 33, 329
Fsp4HI GCNGC 2 cut(s) 189, 422
FspBI CTAG 2 cut(s) 158, 436
GluI GCNGC 2 cut(s) 189, 422
GsuI CTGGAG 1 cut(s) 372
HaeIII GGCC 1 cut(s) 132
Hin1II CATG 3 cut(s) 80, 157, 260
Hpy166II GTNNAC 1 cut(s) 245
Hpy188I TCNGA 2 cut(s) 214, 225
Hpy8I GTNNAC 1 cut(s) 245
HpyAV CCTTC 1 cut(s) 202
HpyCH4IV ACGT 1 cut(s) 112
HpyCH4V TGCA 3 cut(s) 287, 409, 421
HpyF3I CTNAG 1 cut(s) 162
HpySE526I ACGT 1 cut(s) 112
Hsp92II CATG 3 cut(s) 80, 157, 260
Kzo9I GATC 1 cut(s) 103
LmnI GCTCC 2 cut(s) 353, 393
LpnPI CCDG 7 cut(s) 123, 129, 150, 303, 336, 383, 419
Lsp1109I GCAGC 2 cut(s) 175, 433
LweI GCATC 3 cut(s) 11, 307, 331
MaeI CTAG 2 cut(s) 158, 436
MaeII ACGT 1 cut(s) 112
MalI GATC 1 cut(s) 105
MboI GATC 1 cut(s) 103
MflI RGATCY 1 cut(s) 103
MluCI AATT 2 cut(s) 215, 375
MnlI CCTC 3 cut(s) 117, 304, 401
MroXI GAANNNNTTC 1 cut(s) 219
MspR9I CCNGG 1 cut(s) 138
Mva1269I GAATGC 1 cut(s) 289
MvaI CCWGG 1 cut(s) 138
NdeII GATC 1 cut(s) 103
NheI GCTAGC 1 cut(s) 157
NlaIII CATG 3 cut(s) 80, 157, 260
NlaIV GGNNCC 1 cut(s) 243
PaqCI CACCTGC 1 cut(s) 298
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PctI GAATGC 1 cut(s) 289
PdmI GAANNNNTTC 1 cut(s) 219
PkrI GCNGC 2 cut(s) 190, 423
PmaCI CACGTG 1 cut(s) 113
PmlI CACGTG 1 cut(s) 113
Ppu21I YACGTR 1 cut(s) 113
Psp6I CCWGG 1 cut(s) 136
PspCI CACGTG 1 cut(s) 113
PspGI CCWGG 1 cut(s) 136
PspN4I GGNNCC 1 cut(s) 243
PspPI GGNCC 3 cut(s) 131, 242, 253
PsuI RGATCY 1 cut(s) 103
SatI GCNGC 2 cut(s) 189, 422
Sau3AI GATC 1 cut(s) 103
Sau96I GGNCC 3 cut(s) 131, 242, 253
ScrFI CCNGG 1 cut(s) 138
SfaNI GCATC 3 cut(s) 11, 307, 331
SinI GGWCC 2 cut(s) 242, 253
Sse9I AATT 2 cut(s) 215, 375
SspMI CTAG 2 cut(s) 158, 436
StyD4I CCNGG 1 cut(s) 136
StyI CCWWGG 1 cut(s) 276
TaiI ACGT 1 cut(s) 115
TasI AATT 2 cut(s) 215, 375
TscAI CASTG 1 cut(s) 60
TseI GCWGC 2 cut(s) 188, 421
TspDTI ATGAA 2 cut(s) 184, 398
TspGWI ACGGA 2 cut(s) 35, 257
TspRI CASTG 1 cut(s) 60
VpaK11BI GGWCC 2 cut(s) 242, 253
XagI CCTNNNNNAGG 1 cut(s) 395
XapI RAATTY 2 cut(s) 215, 375
XmnI GAANNNNTTC 1 cut(s) 219
XspI CTAG 2 cut(s) 158, 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.