RLG00000030144

26S protease regulatory subunit 6B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002106
Physical Location & Seq
Reverse (-)
56348443 .. 56352176
3734 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030144

Sequence Viewer

Length: 1260 bp
ATGGCAGCGTCAGCGATGGTGTTGGACCCAAAACCCGCGGCGGAGCCACCCGCTTCGTTCCCATCCACAAGATCCGACCTAAAGCTCACTGACCAGCACCAATCCTCCGACGAGGACGACCTCTACAGCCGCCTCAAGTCCCTGCAGCGCCAGCTGGAGTTCATCGATATCCAAGAGGAGTACGTCAAAGACGAGCAGAAGAACCTGAAGCGCGAGCTCCTCCGCGCCCAGGAGGAGGTGAAGAGGATCCAGTCGGTGCCTCTGGTGATCGGACAGTTCATGGAGATGGTGGATCAGAACAACGGCATCGTCGGGTCCACCACCGGCTCGAATTACTATGTCAGGATTCTCAGCACTATTAATCGGGAGCATCTGAAGCCCTCCGCCTCCGTCGCGTTGCACCGCCACTCCAACGCCCTCGTCGACGTTCTGCCGCCGGAGGCCGACTCCAGTATTTCGCTGCTCAGCCAAAGCGAGAAGCCCGACGTCACCTATAACGATATCGGGGGATGTGACATTCAAAAGCAAGAAATCCGTGAAGCAGTGGAACTTCCACTTACACACCATGACTTATACAAACAGATTGGAATAGATCCTCCACGTGGTGTATTGCTGTATGGCCCACCTGGAACTGGTAAAACCATGCTAGCTAAGGCTGTTGCCAATCATACAACTGCTGCCTTCATTAGAGTTGTTGGTTCAGAATTTGTTCAGAAGTATTTGGGTGAGGGTCCACGAATGGTCCGTGATGTTTTCCGTCTCGCCAAGGAGAATGCACCTGCCATCATCTTTATTGATGAGGTGGATGCTATCGCTACTGCAAGGTTTGATGCTCAAACTGGAGCTGATAGAGAAGTTCAGCGAATTCTTATGGAGCTCCTCAATCAGATGGATGGGTTTGACCAGACTGTGAATGTTAAGGTCATAATGGCGACTAATCGTGCTGACACTTTGGATCCTGCACTTCTGCGTCCTGGAAGGCTTGATCGGAAAATTGAATTCCCTTTGCCTGATAGGCGGCAAAAAAGACTTGTTTTTCAGGTTTGCACTGCTAAAATGAACTTGAGTGATGAGGTAGACTTGGAAGATTATGTCTCACGGCCAGATAAAATCAGCGCTGCTGAGATTACAGCTATATGTCAAGAAGCAGGAATGCATGCAGTTCGAAAGAACCGATATGTCATACTGCCAAAGGACTTTGAGAAGGGTTACCAAACCAACGTGAAGAAGCCTGATACTGACTTTGAATTCTACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005911 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0030054 GO:0030162 GO:0030163 GO:0030312 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055044 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

420

Amino Acids

47.15

Weight (kDa)

5.55

Isoelectric Point (pI)

36.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_ATP_ID_OB_C PF16450 90 - 145 1.6e-11 Proteasomal ATPase OB C-terminal domain
AAA_5 PF07728 202 - 324 6.7e-08 AAA domain (dynein-related subfamily)
AAA PF00004 203 - 336 2.1e-45 ATPase family associated with various cellular activities (AAA)
AAA_2 PF07724 203 - 296 2.9e-06 AAA domain (Cdc48 subfamily)
AAA_lid_3 PF17862 359 - 402 6.8e-07 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 787
AatII GACGTC 1 cut(s) 489
Acc36I ACCTGC 1 cut(s) 787
AccB1I GGYRCC 1 cut(s) 256
AccI GTMKAC 2 cut(s) 423, 1077
AccII CGCG 4 cut(s) 38, 213, 225, 395
AclWI GGATC 7 cut(s) 66, 241, 254, 300, 587, 950, 963
AcoI YGGCCR 1 cut(s) 1100
AcsI RAATTY 4 cut(s) 704, 864, 998, 1247
AcuI CTGAAG 2 cut(s) 227, 395
AcvI CACGTG 1 cut(s) 602
AcyI GRCGYC 1 cut(s) 486
AdeI CACNNNGTG 1 cut(s) 605
AfaI GTAC 1 cut(s) 182
AfeI AGCGCT 1 cut(s) 1117
AfiI CCNNNNNNNGG 4 cut(s) 229, 235, 602, 632
AgsI TTSAA 3 cut(s) 521, 998, 1247
AjnI CCWGG 3 cut(s) 228, 625, 973
AluBI AGCT 7 cut(s) 85, 154, 217, 650, 845, 877, 1133
AluI AGCT 7 cut(s) 85, 154, 217, 650, 845, 877, 1133
Alw21I GWGCWC 2 cut(s) 219, 879
Alw26I GTCTC 2 cut(s) 764, 1099
AlwI GGATC 7 cut(s) 66, 241, 254, 300, 587, 950, 963
Aor51HI AGCGCT 1 cut(s) 1117
AoxI GGCC 3 cut(s) 441, 619, 1100
ApeKI GCWGC 5 cut(s) 5, 145, 460, 677, 1118
ApoI RAATTY 4 cut(s) 704, 864, 998, 1247
AseI ATTAAT 1 cut(s) 360
Asp700I GAANNNNTTC 1 cut(s) 708
AspLEI GCGC 4 cut(s) 150, 213, 227, 1118
AspS9I GGNCC 5 cut(s) 25, 315, 620, 731, 742
AsuHPI GGTGA 4 cut(s) 250, 277, 481, 737
AsuII TTCGAA 1 cut(s) 1165
AsuNHI GCTAGC 1 cut(s) 646
AvaII GGWCC 4 cut(s) 25, 315, 731, 742
BamHI GGATCC 2 cut(s) 246, 955
BanI GGYRCC 1 cut(s) 256
BanII GRGCYC 2 cut(s) 219, 879
BbrPI CACGTG 1 cut(s) 602
Bbv12I GWGCWC 2 cut(s) 219, 879
BbvI GCAGC 5 cut(s) 17, 157, 447, 664, 1105
BccI CCATC 6 cut(s) 10, 70, 280, 791, 883, 887
BceAI ACGGC 2 cut(s) 319, 1115
BcgI CGANNNNNNTGC 2 cut(s) 1145, 1179
BciT130I CCWGG 3 cut(s) 230, 627, 975
BcoDI GTCTC 2 cut(s) 764, 1099
BfaI CTAG 1 cut(s) 647
BfmI CTRYAG 2 cut(s) 124, 143
BfoI RGCGCY 2 cut(s) 151, 1119
BfuAI ACCTGC 1 cut(s) 787
BglI GCCNNNNNGGC 1 cut(s) 1015
BisI GCNGC 9 cut(s) 6, 39, 130, 146, 434, 461, 678, 1019, 1119
BlpI GCTNAGC 1 cut(s) 464
BlsI GCNGC 9 cut(s) 7, 40, 131, 147, 435, 462, 679, 1020, 1120
Bme1390I CCNGG 3 cut(s) 230, 627, 975
Bme18I GGWCC 4 cut(s) 25, 315, 731, 742
BmgT120I GGNCC 5 cut(s) 25, 315, 620, 731, 742
BmiI GGNNCC 7 cut(s) 27, 45, 248, 258, 316, 732, 957
BmrFI CCNGG 3 cut(s) 230, 627, 975
BmsI GCATC 4 cut(s) 315, 379, 796, 820
BmtI GCTAGC 1 cut(s) 650
BplI GAGNNNNNCTC 2 cut(s) 431, 463
BpmI CTGGAG 3 cut(s) 176, 433, 861
Bpu10I CCTNAGC 1 cut(s) 651
Bpu1102I GCTNAGC 1 cut(s) 464
Bpu14I TTCGAA 1 cut(s) 1165
BpuEI CTTGAG 2 cut(s) 119, 1084
Bsa29I ATCGAT 1 cut(s) 165
BsaAI YACGTR 1 cut(s) 602
BsaHI GRCGYC 1 cut(s) 486
BsaJI CCNNGG 3 cut(s) 36, 228, 765
BsaXI ACNNNNNCTCC 4 cut(s) 89, 119, 392, 422
Bsc4I CCNNNNNNNGG 4 cut(s) 229, 235, 602, 632
Bse118I RCCGGY 1 cut(s) 323
Bse1I ACTGG 4 cut(s) 250, 450, 637, 844
BseBI CCWGG 3 cut(s) 230, 627, 975
BseCI ATCGAT 1 cut(s) 165
BseDI CCNNGG 3 cut(s) 36, 228, 765
BseGI GGATG 4 cut(s) 62, 515, 811, 898
BseLI CCNNNNNNNGG 4 cut(s) 229, 235, 602, 632
BseMII CTCAG 3 cut(s) 364, 478, 1113
BseNI ACTGG 4 cut(s) 250, 450, 637, 844
BseRI GAGGAG 4 cut(s) 191, 209, 248, 869
BseXI GCAGC 5 cut(s) 17, 157, 447, 664, 1105
BsgI GTGCAG 1 cut(s) 945
Bsh1236I CGCG 4 cut(s) 38, 213, 225, 395
BshFI GGCC 3 cut(s) 443, 621, 1102
BshNI GGYRCC 1 cut(s) 256
BshVI ATCGAT 1 cut(s) 165
BsiHKAI GWGCWC 2 cut(s) 219, 879
BsiSI CCGG 2 cut(s) 324, 437
BslFI GGGAC 1 cut(s) 124
BslI CCNNNNNNNGG 4 cut(s) 229, 235, 602, 632
BsmAI GTCTC 2 cut(s) 764, 1099
BsmBI CGTCTC 1 cut(s) 764
BsmFI GGGAC 1 cut(s) 124
BsmI GAATGC 2 cut(s) 778, 1158
BsnI GGCC 3 cut(s) 443, 621, 1102
Bsp119I TTCGAA 1 cut(s) 1165
Bsp1286I GDGCHC 2 cut(s) 219, 879
Bsp143I GATC 7 cut(s) 71, 246, 267, 292, 592, 955, 985
Bsp1720I GCTNAGC 1 cut(s) 464
BspANI GGCC 3 cut(s) 443, 621, 1102
BspCNI CTCAG 3 cut(s) 363, 477, 1114
BspDI ATCGAT 1 cut(s) 165
BspFNI CGCG 4 cut(s) 38, 213, 225, 395
BspLI GGNNCC 7 cut(s) 27, 45, 248, 258, 316, 732, 957
BspMAI CTGCAG 1 cut(s) 147
BspMI ACCTGC 1 cut(s) 787
BspOI GCTAGC 1 cut(s) 650
BspPI GGATC 7 cut(s) 66, 241, 254, 300, 587, 950, 963
BspT104I TTCGAA 1 cut(s) 1165
BspT107I GGYRCC 1 cut(s) 256
BsrFI RCCGGY 1 cut(s) 323
BsrI ACTGG 4 cut(s) 250, 450, 637, 844
BssAI RCCGGY 1 cut(s) 323
BssECI CCNNGG 3 cut(s) 36, 228, 765
BssMI GATC 7 cut(s) 71, 246, 267, 292, 592, 955, 985
BssNI GRCGYC 1 cut(s) 486
BssT1I CCWWGG 1 cut(s) 765
Bst2UI CCWGG 3 cut(s) 230, 627, 975
Bst4CI ACNGT 2 cut(s) 276, 910
Bst6I CTCTTC 1 cut(s) 236
BstACI GRCGYC 1 cut(s) 486
BstBAI YACGTR 1 cut(s) 602
BstBI TTCGAA 1 cut(s) 1165
BstC8I GCNNGC 4 cut(s) 152, 215, 648, 1158
BstDEI CTNAG 4 cut(s) 350, 464, 651, 1122
BstDSI CCRYGG 1 cut(s) 36
BstEII GGTNACC 1 cut(s) 1208
BstF5I GGATG 4 cut(s) 62, 515, 811, 898
BstFNI CGCG 4 cut(s) 38, 213, 225, 395
BstH2I RGCGCY 2 cut(s) 151, 1119
BstHHI GCGC 4 cut(s) 150, 213, 227, 1118
BstKTI GATC 7 cut(s) 74, 249, 270, 295, 595, 958, 988
BstMAI GTCTC 2 cut(s) 764, 1099
BstMBI GATC 7 cut(s) 71, 246, 267, 292, 592, 955, 985
BstMWI GCNNNNNNNGC 5 cut(s) 11, 151, 376, 392, 1015
BstNI CCWGG 3 cut(s) 230, 627, 975
BstNSI RCATGY 1 cut(s) 1160
BstPI GGTNACC 1 cut(s) 1208
BstSCI CCNGG 3 cut(s) 228, 625, 973
BstSFI CTRYAG 2 cut(s) 124, 143
BstUI CGCG 4 cut(s) 38, 213, 225, 395
BstV1I GCAGC 5 cut(s) 17, 157, 447, 664, 1105
BstX2I RGATCY 4 cut(s) 71, 246, 592, 955
BstYI RGATCY 4 cut(s) 71, 246, 592, 955
Bsu15I ATCGAT 1 cut(s) 165
BsuRI GGCC 3 cut(s) 443, 621, 1102
BsuTUI ATCGAT 1 cut(s) 165
BtgI CCRYGG 1 cut(s) 36
BtgZI GCGATG 1 cut(s) 29
BtsCI GGATG 4 cut(s) 62, 515, 811, 898
BtsI GCAGTG 2 cut(s) 549, 1047
BtsIMutI CAGTG 3 cut(s) 87, 549, 1047
BveI ACCTGC 1 cut(s) 787
Cac8I GCNNGC 4 cut(s) 152, 215, 648, 1158
CfoI GCGC 4 cut(s) 150, 213, 227, 1118
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 5 cut(s) 25, 315, 620, 731, 742
Cfr42I CCGCGG 1 cut(s) 39
ClaI ATCGAT 1 cut(s) 165
CseI GACGC 1 cut(s) 959
Csp6I GTAC 1 cut(s) 181
CviAII CATG 4 cut(s) 280, 566, 643, 1157
CviQI GTAC 1 cut(s) 181
DdeI CTNAG 4 cut(s) 350, 464, 651, 1122
DpnI GATC 7 cut(s) 73, 248, 269, 294, 594, 957, 987
DpnII GATC 7 cut(s) 71, 246, 267, 292, 592, 955, 985
DraIII CACNNNGTG 1 cut(s) 605
EaeI YGGCCR 1 cut(s) 1100
Eam1104I CTCTTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 236
EciI GGCGGA 2 cut(s) 56, 373
Ecl136II GAGCTC 2 cut(s) 217, 877
Eco130I CCWWGG 1 cut(s) 765
Eco24I GRGCYC 2 cut(s) 219, 879
Eco32I GATATC 2 cut(s) 169, 502
Eco47I GGWCC 4 cut(s) 25, 315, 731, 742
Eco47III AGCGCT 1 cut(s) 1117
Eco53kI GAGCTC 2 cut(s) 217, 877
Eco57I CTGAAG 2 cut(s) 227, 395
Eco72I CACGTG 1 cut(s) 602
Eco91I GGTNACC 1 cut(s) 1208
EcoICRI GAGCTC 2 cut(s) 217, 877
EcoO65I GGTNACC 1 cut(s) 1208
EcoRI GAATTC 3 cut(s) 864, 998, 1247
EcoRII CCWGG 3 cut(s) 228, 625, 973
EcoRV GATATC 2 cut(s) 169, 502
EcoT14I CCWWGG 1 cut(s) 765
EcoT22I ATGCAT 1 cut(s) 1158
EcoT38I GRGCYC 2 cut(s) 219, 879
ErhI CCWWGG 1 cut(s) 765
Esp3I CGTCTC 1 cut(s) 764
FaeI CATG 4 cut(s) 283, 569, 646, 1160
FaqI GGGAC 1 cut(s) 124
FatI CATG 4 cut(s) 279, 565, 642, 1156
FauI CCCGC 2 cut(s) 43, 58
FblI GTMKAC 2 cut(s) 423, 1077
Fnu4HI GCNGC 9 cut(s) 6, 39, 130, 146, 434, 461, 678, 1019, 1119
FokI GGATG 4 cut(s) 49, 522, 818, 905
FriOI GRGCYC 2 cut(s) 219, 879
Fsp4HI GCNGC 9 cut(s) 6, 39, 130, 146, 434, 461, 678, 1019, 1119
FspBI CTAG 1 cut(s) 647
GlaI GCGC 4 cut(s) 149, 212, 226, 1117
GluI GCNGC 9 cut(s) 6, 39, 130, 146, 434, 461, 678, 1019, 1119
GsuI CTGGAG 3 cut(s) 176, 433, 861
HaeII RGCGCY 2 cut(s) 151, 1119
HaeIII GGCC 3 cut(s) 443, 621, 1102
HapII CCGG 2 cut(s) 324, 437
HgaI GACGC 1 cut(s) 959
HhaI GCGC 4 cut(s) 150, 213, 227, 1118
Hin1I GRCGYC 1 cut(s) 486
Hin1II CATG 4 cut(s) 283, 569, 646, 1160
Hin6I GCGC 4 cut(s) 148, 211, 225, 1116
HinP1I GCGC 4 cut(s) 148, 211, 225, 1116
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HinfI GANTC 2 cut(s) 346, 446
HpaII CCGG 2 cut(s) 324, 437
HphI GGTGA 4 cut(s) 250, 277, 481, 737
Hpy166II GTNNAC 4 cut(s) 318, 424, 734, 1078
Hpy188I TCNGA 9 cut(s) 76, 109, 272, 297, 375, 703, 714, 888, 990
Hpy188III TCNNGA 3 cut(s) 343, 365, 1142
Hpy8I GTNNAC 4 cut(s) 318, 424, 734, 1078
Hpy99I CGWCG 6 cut(s) 113, 314, 395, 425, 428, 488
HpyAV CCTTC 3 cut(s) 691, 972, 1198
HpyCH4III ACNGT 2 cut(s) 276, 910
HpyCH4IV ACGT 5 cut(s) 183, 426, 486, 601, 1221
HpyCH4V TGCA 8 cut(s) 145, 400, 776, 821, 962, 1047, 1156, 1160
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 151, 376, 392, 1015
HpyF3I CTNAG 4 cut(s) 350, 464, 651, 1122
HpySE526I ACGT 5 cut(s) 183, 426, 486, 601, 1221
Hsp92I GRCGYC 1 cut(s) 486
Hsp92II CATG 4 cut(s) 283, 569, 646, 1160
HspAI GCGC 4 cut(s) 148, 211, 225, 1116
KspI CCGCGG 1 cut(s) 39
Kzo9I GATC 7 cut(s) 71, 246, 267, 292, 592, 955, 985
LmnI GCTCC 6 cut(s) 43, 222, 367, 842, 874, 882
Lsp1109I GCAGC 5 cut(s) 17, 157, 447, 664, 1105
LweI GCATC 4 cut(s) 315, 379, 796, 820
MaeI CTAG 1 cut(s) 647
MaeII ACGT 5 cut(s) 183, 426, 486, 601, 1221
MaeIII GTNAC 3 cut(s) 487, 512, 1208
MalI GATC 7 cut(s) 73, 248, 269, 294, 594, 957, 987
MboI GATC 7 cut(s) 71, 246, 267, 292, 592, 955, 985
MboII GAAGA 4 cut(s) 211, 253, 1097, 1237
MflI RGATCY 4 cut(s) 71, 246, 592, 955
MhlI GDGCHC 2 cut(s) 219, 879
MluCI AATT 6 cut(s) 331, 704, 864, 993, 998, 1247
MlyI GAGTC 1 cut(s) 440
MmeI TCCRAC 3 cut(s) 99, 132, 435
Mph1103I ATGCAT 1 cut(s) 1158
MroXI GAANNNNTTC 1 cut(s) 708
MseI TTAA 2 cut(s) 360, 918
MslI CAYNNNNRTG 1 cut(s) 284
MspA1I CMGCKG 2 cut(s) 38, 154
MspI CCGG 2 cut(s) 324, 437
MspR9I CCNGG 3 cut(s) 230, 627, 975
Mva1269I GAATGC 2 cut(s) 778, 1158
MvaI CCWGG 3 cut(s) 230, 627, 975
MvnI CGCG 4 cut(s) 38, 213, 225, 395
MwoI GCNNNNNNNGC 5 cut(s) 11, 151, 376, 392, 1015
NdeII GATC 7 cut(s) 71, 246, 267, 292, 592, 955, 985
NheI GCTAGC 1 cut(s) 646
NlaIII CATG 4 cut(s) 283, 569, 646, 1160
NlaIV GGNNCC 7 cut(s) 27, 45, 248, 258, 316, 732, 957
NmuCI GTSAC 2 cut(s) 487, 512
NsiI ATGCAT 1 cut(s) 1158
NspI RCATGY 1 cut(s) 1160
NspV TTCGAA 1 cut(s) 1165
PaeI GCATGC 1 cut(s) 1160
PaqCI CACCTGC 1 cut(s) 787
PcsI WCGNNNNNNNCGW 4 cut(s) 189, 420, 742, 1171
PctI GAATGC 2 cut(s) 778, 1158
PdmI GAANNNNTTC 1 cut(s) 708
PfeI GAWTC 1 cut(s) 346
PfoI TCCNGGA 1 cut(s) 973
PkrI GCNGC 9 cut(s) 7, 40, 131, 147, 435, 462, 679, 1020, 1120
PleI GAGTC 1 cut(s) 440
PmaCI CACGTG 1 cut(s) 602
PmlI CACGTG 1 cut(s) 602
PpsI GAGTC 1 cut(s) 440
Ppu21I YACGTR 1 cut(s) 602
PshBI ATTAAT 1 cut(s) 360
Psp124BI GAGCTC 2 cut(s) 219, 879
Psp6I CCWGG 3 cut(s) 228, 625, 973
PspCI CACGTG 1 cut(s) 602
PspEI GGTNACC 1 cut(s) 1208
PspGI CCWGG 3 cut(s) 228, 625, 973
PspN4I GGNNCC 7 cut(s) 27, 45, 248, 258, 316, 732, 957
PspPI GGNCC 5 cut(s) 25, 315, 620, 731, 742
PstI CTGCAG 1 cut(s) 147
PsuI RGATCY 4 cut(s) 71, 246, 592, 955
PvuII CAGCTG 1 cut(s) 154
RsaI GTAC 1 cut(s) 182
RsaNI GTAC 1 cut(s) 181
RseI CAYNNNNRTG 1 cut(s) 284
SacI GAGCTC 2 cut(s) 219, 879
SacII CCGCGG 1 cut(s) 39
SalI GTCGAC 1 cut(s) 422
SaqAI TTAA 2 cut(s) 360, 918
SatI GCNGC 9 cut(s) 6, 39, 130, 146, 434, 461, 678, 1019, 1119
Sau3AI GATC 7 cut(s) 71, 246, 267, 292, 592, 955, 985
Sau96I GGNCC 5 cut(s) 25, 315, 620, 731, 742
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 3 cut(s) 230, 627, 975
SduI GDGCHC 2 cut(s) 219, 879
SfaNI GCATC 4 cut(s) 315, 379, 796, 820
SfcI CTRYAG 2 cut(s) 124, 143
Sfr303I CCGCGG 1 cut(s) 39
SfuI TTCGAA 1 cut(s) 1165
SgrBI CCGCGG 1 cut(s) 39
SgrDI CGTCGACG 1 cut(s) 422
SinI GGWCC 4 cut(s) 25, 315, 731, 742
SmiMI CAYNNNNRTG 1 cut(s) 284
SmlI CTYRAG 2 cut(s) 134, 1063
SmoI CTYRAG 2 cut(s) 134, 1063
SphI GCATGC 1 cut(s) 1160
Sse9I AATT 6 cut(s) 331, 704, 864, 993, 998, 1247
SspMI CTAG 1 cut(s) 647
SstI GAGCTC 2 cut(s) 219, 879
StyD4I CCNGG 3 cut(s) 228, 625, 973
StyI CCWWGG 1 cut(s) 765
TaaI ACNGT 2 cut(s) 276, 910
TaiI ACGT 5 cut(s) 186, 429, 489, 604, 1224
TaqI TCGA 4 cut(s) 165, 329, 423, 1165
TasI AATT 6 cut(s) 331, 704, 864, 993, 998, 1247
TauI GCSGC 4 cut(s) 41, 132, 436, 1021
TfiI GAWTC 1 cut(s) 346
Tru1I TTAA 2 cut(s) 360, 918
Tru9I TTAA 2 cut(s) 360, 918
TscAI CASTG 3 cut(s) 94, 549, 1054
TseFI GTSAC 2 cut(s) 487, 512
TseI GCWGC 5 cut(s) 5, 145, 460, 677, 1118
Tsp45I GTSAC 2 cut(s) 487, 512
TspDTI ATGAA 4 cut(s) 151, 268, 673, 1073
TspGWI ACGGA 4 cut(s) 379, 524, 734, 746
TspRI CASTG 3 cut(s) 94, 549, 1054
VpaK11BI GGWCC 4 cut(s) 25, 315, 731, 742
VspI ATTAAT 1 cut(s) 360
XapI RAATTY 4 cut(s) 704, 864, 998, 1247
XceI RCATGY 1 cut(s) 1160
XmiI GTMKAC 2 cut(s) 423, 1077
XmnI GAANNNNTTC 1 cut(s) 708
XspI CTAG 1 cut(s) 647
ZraI GACGTC 1 cut(s) 487
Zsp2I ATGCAT 1 cut(s) 1158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.