pycom05g02220

26S protease regulatory subunit 6B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002106
Physical Location & Seq
Forward (+)
2563728 .. 2567376
3649 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g02220.1

Sequence Viewer

Length: 1263 bp
ATGTCAGGATCAGCCATGGTGTTGGATCCGAAACCTTTGCCTGAGCCACCAGCTTCAATCCCCTCCATAAGATTGGACCTTCAGGGCTACACCGACCAAAACCAGGCCTCCGATGAGGACGATCTCTACAGCCGCCTCAAGTCCTATCAGCGACAGCTCGAGTTCATCGACATCCAGGAGGAGTATGTCAAGGACGAGCAGAAGAATCTGAAGCGTGAGCTCCTCCGCGCCCAGGAAGAAGTCAAGAGGATCCAATCGGTGCCGCTTGTCATCGGGCAATTCATGGAGATGGTCGACCAGAACAACGGGATTGTGGGCTCCACCACTGGCTCCAATTACTATGTTAGGATTCTCAGCACCATTAATCGGGAGCTCCTCAAGCCTTCTGCTTCCGTTGCCTTGCACCGCCACTCCAATGCCCTCGTGGACGTTTTGCCTCCGGAGGCCGACTCAAGTATTTCCCTCCTCAGCCAGTCTGAGAAGCCCGATGTCACCTACTCTGATATTGGAGGATGTGACATTCAGAAGCAAGAAATCCGTGAAGCAGTAGAACTGCCACTTACCCATCACGAGTTGTACAAACAGATTGGAATAGATCCCCCACGTGGTGTATTGCTTTATGGTCCACCTGGAACTGGTAAAACCATGTTAGCAAAGGCCGTGGCTAATCATACAACTGCTGCCTTCATCAGAGTTGTTGGTTCAGAATTTGTTCAGAAGTATTTGGGAGAGGGTCCACGAATGGTTCGTGATGTTTTCCGTCTTGCCAAGGAGAATGCACCTGCCATCATCTTTATTGATGAGGTGGATGCTATTGCTACTGCAAGGTTTGATGCTCAAACTGGAGCTGACAGAGAAGTTCAGAGAATCCTTATGGAGCTCCTTAATCAGATGGATGGGTTTGACCAGACAGTGAATGTTAAGGTTATTATGGCTACTAACCGAGCAGACACCTTGGATCCTGCACTTGTGCGTCCTGGAAGGCTTGATCGGAAGATTGAGTTTCCTTTGCCTGATAGGAGGCAGAAAAGACTTGTTTTTCAGGTTTGCACCGCTAAAATGAACTTGAGTGATGAGGTAGACTTGGAAGATTATGTCTCTCGGCCAGACAAAATCAGTGCTGCTGAGATTTCAGCTATATGTCAAGAAGCAGGAATGCATGCAGTTCGGAAGAACCGATATGTCATACTCCCAAAGGACTTTGAGAAGGGTTACCAAACCAACGTGAAGAAGCCCGACACTGACTTTGAATTCTACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005911 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0030054 GO:0030162 GO:0030163 GO:0030312 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055044 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

421

Amino Acids

47.28

Weight (kDa)

5.33

Isoelectric Point (pI)

34.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_ATP_ID_OB_C PF16450 91 - 146 8.8e-12 Proteasomal ATPase OB C-terminal domain
AAA_5 PF07728 203 - 325 6.8e-08 AAA domain (dynein-related subfamily)
AAA PF00004 204 - 337 3.5e-45 ATPase family associated with various cellular activities (AAA)
AAA_2 PF07724 204 - 297 2.9e-06 AAA domain (Cdc48 subfamily)
AAA_lid_3 PF17862 360 - 403 6e-07 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 790
Acc36I ACCTGC 1 cut(s) 790
AccB1I GGYRCC 1 cut(s) 259
AccI GTMKAC 2 cut(s) 294, 1080
AccII CGCG 1 cut(s) 228
AccIII TCCGGA 1 cut(s) 439
AciI CCGC 5 cut(s) 133, 226, 263, 406, 1053
AclWI GGATC 8 cut(s) 16, 20, 33, 244, 257, 590, 953, 966
AcoI YGGCCR 1 cut(s) 1103
AcsI RAATTY 2 cut(s) 707, 1250
AcuI CTGAAG 2 cut(s) 65, 230
AcvI CACGTG 1 cut(s) 605
AdeI CACNNNGTG 1 cut(s) 608
AfaI GTAC 1 cut(s) 578
AfiI CCNNNNNNNGG 5 cut(s) 103, 232, 366, 605, 635
AgsI TTSAA 2 cut(s) 57, 1250
AjnI CCWGG 5 cut(s) 102, 174, 231, 628, 976
AluBI AGCT 7 cut(s) 53, 157, 220, 373, 848, 880, 1136
AluI AGCT 7 cut(s) 53, 157, 220, 373, 848, 880, 1136
Alw21I GWGCWC 3 cut(s) 222, 375, 882
Alw26I GTCTC 1 cut(s) 1102
AlwI GGATC 8 cut(s) 16, 20, 33, 244, 257, 590, 953, 966
Ama87I CYCGRG 1 cut(s) 158
Aor13HI TCCGGA 1 cut(s) 439
AoxI GGCC 4 cut(s) 105, 444, 657, 1103
ApeKI GCWGC 2 cut(s) 680, 1121
ApoI RAATTY 2 cut(s) 707, 1250
ArsI GACNNNNNNTTYG 2 cut(s) 1229, 1261
AseI ATTAAT 1 cut(s) 363
Asp700I GAANNNNTTC 1 cut(s) 711
AspLEI GCGC 1 cut(s) 230
AspS9I GGNCC 3 cut(s) 76, 623, 734
AsuHPI GGTGA 1 cut(s) 484
AvaI CYCGRG 1 cut(s) 158
AvaII GGWCC 3 cut(s) 76, 623, 734
BamHI GGATCC 3 cut(s) 25, 249, 958
BanI GGYRCC 1 cut(s) 259
BanII GRGCYC 4 cut(s) 222, 320, 375, 882
BauI CACGAG 2 cut(s) 422, 569
BbrPI CACGTG 1 cut(s) 605
Bbv12I GWGCWC 3 cut(s) 222, 375, 882
BbvCI CCTCAGC 1 cut(s) 467
BbvI GCAGC 2 cut(s) 667, 1108
BccI CCATC 5 cut(s) 283, 573, 794, 886, 890
BceAI ACGGC 1 cut(s) 644
BcgI CGANNNNNNTGC 4 cut(s) 19, 53, 1148, 1182
BciT130I CCWGG 5 cut(s) 104, 176, 233, 630, 978
BcoDI GTCTC 1 cut(s) 1102
BfmI CTRYAG 1 cut(s) 127
BfuAI ACCTGC 1 cut(s) 790
BisI GCNGC 4 cut(s) 133, 263, 681, 1122
BlsI GCNGC 4 cut(s) 134, 264, 682, 1123
Bme1390I CCNGG 5 cut(s) 104, 176, 233, 630, 978
Bme18I GGWCC 3 cut(s) 76, 623, 734
BmeT110I CYCGRG 1 cut(s) 158
BmgT120I GGNCC 3 cut(s) 76, 623, 734
BmiI GGNNCC 7 cut(s) 27, 251, 261, 319, 331, 735, 960
BmrFI CCNGG 5 cut(s) 104, 176, 233, 630, 978
BmsI GCATC 2 cut(s) 799, 823
BplI GAGNNNNNCTC 2 cut(s) 434, 466
BpmI CTGGAG 1 cut(s) 864
Bpu10I CCTNAGC 2 cut(s) 42, 467
BpuEI CTTGAG 4 cut(s) 122, 362, 436, 1087
BsaAI YACGTR 1 cut(s) 605
BsaJI CCNNGG 5 cut(s) 15, 231, 660, 768, 954
BsaWI WCCGGW 1 cut(s) 439
BsaXI ACNNNNNCTCC 4 cut(s) 92, 122, 395, 425
Bsc4I CCNNNNNNNGG 5 cut(s) 103, 232, 366, 605, 635
Bse1I ACTGG 4 cut(s) 331, 472, 640, 847
BseAI TCCGGA 1 cut(s) 439
BseBI CCWGG 5 cut(s) 104, 176, 233, 630, 978
BseDI CCNNGG 5 cut(s) 15, 231, 660, 768, 954
BseGI GGATG 4 cut(s) 171, 518, 814, 901
BseLI CCNNNNNNNGG 5 cut(s) 103, 232, 366, 605, 635
BseMII CTCAG 5 cut(s) 33, 367, 468, 481, 1116
BseNI ACTGG 4 cut(s) 331, 472, 640, 847
BseRI GAGGAG 4 cut(s) 194, 212, 365, 455
BseXI GCAGC 2 cut(s) 667, 1108
BsgI GTGCAG 1 cut(s) 948
Bsh1236I CGCG 1 cut(s) 228
BshFI GGCC 4 cut(s) 107, 446, 659, 1105
BshNI GGYRCC 1 cut(s) 259
BsiHKAI GWGCWC 3 cut(s) 222, 375, 882
BsiHKCI CYCGRG 1 cut(s) 158
BsiSI CCGG 1 cut(s) 440
BslI CCNNNNNNNGG 5 cut(s) 103, 232, 366, 605, 635
BsmAI GTCTC 1 cut(s) 1102
BsmI GAATGC 2 cut(s) 781, 1161
BsnI GGCC 4 cut(s) 107, 446, 659, 1105
BsoBI CYCGRG 1 cut(s) 158
Bsp1286I GDGCHC 4 cut(s) 222, 320, 375, 882
Bsp13I TCCGGA 1 cut(s) 439
Bsp1407I TGTACA 1 cut(s) 576
Bsp143I GATC 7 cut(s) 8, 25, 121, 249, 595, 958, 988
Bsp19I CCATGG 1 cut(s) 15
BspACI CCGC 5 cut(s) 133, 226, 263, 406, 1053
BspANI GGCC 4 cut(s) 107, 446, 659, 1105
BspCNI CTCAG 5 cut(s) 34, 366, 469, 480, 1117
BspEI TCCGGA 1 cut(s) 439
BspFNI CGCG 1 cut(s) 228
BspLI GGNNCC 7 cut(s) 27, 251, 261, 319, 331, 735, 960
BspMI ACCTGC 1 cut(s) 790
BspPI GGATC 8 cut(s) 16, 20, 33, 244, 257, 590, 953, 966
BspT107I GGYRCC 1 cut(s) 259
BsrGI TGTACA 1 cut(s) 576
BsrI ACTGG 4 cut(s) 331, 472, 640, 847
BssECI CCNNGG 5 cut(s) 15, 231, 660, 768, 954
BssMI GATC 7 cut(s) 8, 25, 121, 249, 595, 958, 988
BssSI CACGAG 2 cut(s) 422, 569
BssT1I CCWWGG 3 cut(s) 15, 768, 954
Bst2BI CACGAG 2 cut(s) 422, 569
Bst2UI CCWGG 5 cut(s) 104, 176, 233, 630, 978
Bst4CI ACNGT 1 cut(s) 913
BstAUI TGTACA 1 cut(s) 576
BstBAI YACGTR 1 cut(s) 605
BstC8I GCNNGC 1 cut(s) 1161
BstDEI CTNAG 5 cut(s) 42, 353, 467, 477, 1125
BstDSI CCRYGG 2 cut(s) 15, 660
BstEII GGTNACC 1 cut(s) 1211
BstF5I GGATG 4 cut(s) 171, 518, 814, 901
BstFNI CGCG 1 cut(s) 228
BstHHI GCGC 1 cut(s) 230
BstKTI GATC 7 cut(s) 11, 28, 124, 252, 598, 961, 991
BstMAI GTCTC 1 cut(s) 1102
BstMBI GATC 7 cut(s) 8, 25, 121, 249, 595, 958, 988
BstMWI GCNNNNNNNGC 2 cut(s) 379, 395
BstNI CCWGG 5 cut(s) 104, 176, 233, 630, 978
BstNSI RCATGY 1 cut(s) 1163
BstPI GGTNACC 1 cut(s) 1211
BstSCI CCNGG 5 cut(s) 102, 174, 231, 628, 976
BstSFI CTRYAG 1 cut(s) 127
BstUI CGCG 1 cut(s) 228
BstV1I GCAGC 2 cut(s) 667, 1108
BstX2I RGATCY 4 cut(s) 25, 249, 595, 958
BstXI CCANNNNNNTGG 2 cut(s) 22, 73
BstYI RGATCY 4 cut(s) 25, 249, 595, 958
BsuRI GGCC 4 cut(s) 107, 446, 659, 1105
BtgI CCRYGG 2 cut(s) 15, 660
BtsCI GGATG 4 cut(s) 171, 518, 814, 901
BtsIMutI CAGTG 4 cut(s) 324, 918, 1123, 1239
BveI ACCTGC 1 cut(s) 790
Cac8I GCNNGC 1 cut(s) 1161
CfoI GCGC 1 cut(s) 230
Cfr13I GGNCC 3 cut(s) 76, 623, 734
CseI GACGC 1 cut(s) 962
Csp6I GTAC 1 cut(s) 577
CspCI CAANNNNNGTGG 2 cut(s) 642, 677
CviAII CATG 4 cut(s) 16, 283, 646, 1160
CviQI GTAC 1 cut(s) 577
DdeI CTNAG 5 cut(s) 42, 353, 467, 477, 1125
DpnI GATC 7 cut(s) 10, 27, 123, 251, 597, 960, 990
DpnII GATC 7 cut(s) 8, 25, 121, 249, 595, 958, 988
DraIII CACNNNGTG 1 cut(s) 608
EaeI YGGCCR 1 cut(s) 1103
Ecl136II GAGCTC 3 cut(s) 220, 373, 880
Eco130I CCWWGG 3 cut(s) 15, 768, 954
Eco147I AGGCCT 1 cut(s) 107
Eco24I GRGCYC 4 cut(s) 222, 320, 375, 882
Eco47I GGWCC 3 cut(s) 76, 623, 734
Eco53kI GAGCTC 3 cut(s) 220, 373, 880
Eco57I CTGAAG 2 cut(s) 65, 230
Eco72I CACGTG 1 cut(s) 605
Eco88I CYCGRG 1 cut(s) 158
Eco91I GGTNACC 1 cut(s) 1211
EcoICRI GAGCTC 3 cut(s) 220, 373, 880
EcoO65I GGTNACC 1 cut(s) 1211
EcoRI GAATTC 1 cut(s) 1250
EcoRII CCWGG 5 cut(s) 102, 174, 231, 628, 976
EcoT14I CCWWGG 3 cut(s) 15, 768, 954
EcoT22I ATGCAT 1 cut(s) 1161
EcoT38I GRGCYC 4 cut(s) 222, 320, 375, 882
ErhI CCWWGG 3 cut(s) 15, 768, 954
FaeI CATG 4 cut(s) 19, 286, 649, 1163
FatI CATG 4 cut(s) 15, 282, 645, 1159
FblI GTMKAC 2 cut(s) 294, 1080
Fnu4HI GCNGC 4 cut(s) 133, 263, 681, 1122
FokI GGATG 4 cut(s) 158, 525, 821, 908
FriOI GRGCYC 4 cut(s) 222, 320, 375, 882
Fsp4HI GCNGC 4 cut(s) 133, 263, 681, 1122
GlaI GCGC 1 cut(s) 229
GluI GCNGC 4 cut(s) 133, 263, 681, 1122
GsuI CTGGAG 1 cut(s) 864
HaeIII GGCC 4 cut(s) 107, 446, 659, 1105
HapII CCGG 1 cut(s) 440
HgaI GACGC 1 cut(s) 962
HhaI GCGC 1 cut(s) 230
Hin1II CATG 4 cut(s) 19, 286, 649, 1163
Hin6I GCGC 1 cut(s) 228
HinP1I GCGC 1 cut(s) 228
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 4 cut(s) 205, 349, 449, 867
HpaII CCGG 1 cut(s) 440
HphI GGTGA 1 cut(s) 484
Hpy166II GTNNAC 5 cut(s) 295, 427, 626, 737, 1081
Hpy188III TCNNGA 7 cut(s) 6, 244, 368, 440, 569, 749, 1145
Hpy8I GTNNAC 5 cut(s) 295, 427, 626, 737, 1081
HpyAV CCTTC 5 cut(s) 89, 393, 694, 975, 1201
HpyCH4III ACNGT 1 cut(s) 913
HpyCH4IV ACGT 3 cut(s) 429, 604, 1224
HpyCH4V TGCA 7 cut(s) 403, 779, 824, 965, 1050, 1159, 1163
HpyF10VI GCNNNNNNNGC 2 cut(s) 379, 395
HpyF3I CTNAG 5 cut(s) 42, 353, 467, 477, 1125
HpySE526I ACGT 3 cut(s) 429, 604, 1224
Hsp92II CATG 4 cut(s) 19, 286, 649, 1163
HspAI GCGC 1 cut(s) 228
Kpn2I TCCGGA 1 cut(s) 439
Kzo9I GATC 7 cut(s) 8, 25, 121, 249, 595, 958, 988
LmnI GCTCC 8 cut(s) 225, 323, 335, 370, 378, 845, 877, 885
Lsp1109I GCAGC 2 cut(s) 667, 1108
LweI GCATC 2 cut(s) 799, 823
MaeII ACGT 3 cut(s) 429, 604, 1224
MaeIII GTNAC 3 cut(s) 490, 515, 1211
MalI GATC 7 cut(s) 10, 27, 123, 251, 597, 960, 990
MboI GATC 7 cut(s) 8, 25, 121, 249, 595, 958, 988
MboII GAAGA 6 cut(s) 214, 248, 1006, 1100, 1183, 1240
MflI RGATCY 4 cut(s) 25, 249, 595, 958
MhlI GDGCHC 4 cut(s) 222, 320, 375, 882
MluCI AATT 4 cut(s) 278, 334, 707, 1250
MlyI GAGTC 1 cut(s) 443
Mph1103I ATGCAT 1 cut(s) 1161
MroI TCCGGA 1 cut(s) 439
MroXI GAANNNNTTC 1 cut(s) 711
MseI TTAA 3 cut(s) 363, 885, 921
MslI CAYNNNNRTG 2 cut(s) 287, 414
MspI CCGG 1 cut(s) 440
MspR9I CCNGG 5 cut(s) 104, 176, 233, 630, 978
Mva1269I GAATGC 2 cut(s) 781, 1161
MvaI CCWGG 5 cut(s) 104, 176, 233, 630, 978
MvnI CGCG 1 cut(s) 228
MwoI GCNNNNNNNGC 2 cut(s) 379, 395
NcoI CCATGG 1 cut(s) 15
NdeII GATC 7 cut(s) 8, 25, 121, 249, 595, 958, 988
NlaIII CATG 4 cut(s) 19, 286, 649, 1163
NlaIV GGNNCC 7 cut(s) 27, 251, 261, 319, 331, 735, 960
NmeAIII GCCGAG 1 cut(s) 1081
NmuCI GTSAC 2 cut(s) 490, 515
NsiI ATGCAT 1 cut(s) 1161
NspI RCATGY 1 cut(s) 1163
PaeI GCATGC 1 cut(s) 1163
PaeR7I CTCGAG 1 cut(s) 158
PaqCI CACCTGC 1 cut(s) 790
PceI AGGCCT 1 cut(s) 107
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 745, 1174
PctI GAATGC 2 cut(s) 781, 1161
PdmI GAANNNNTTC 1 cut(s) 711
PfeI GAWTC 3 cut(s) 205, 349, 867
PfoI TCCNGGA 2 cut(s) 174, 976
PkrI GCNGC 4 cut(s) 134, 264, 682, 1123
PleI GAGTC 1 cut(s) 443
PmaCI CACGTG 1 cut(s) 605
PmlI CACGTG 1 cut(s) 605
PpsI GAGTC 1 cut(s) 443
Ppu21I YACGTR 1 cut(s) 605
PshBI ATTAAT 1 cut(s) 363
Psp124BI GAGCTC 3 cut(s) 222, 375, 882
Psp6I CCWGG 5 cut(s) 102, 174, 231, 628, 976
PspCI CACGTG 1 cut(s) 605
PspEI GGTNACC 1 cut(s) 1211
PspGI CCWGG 5 cut(s) 102, 174, 231, 628, 976
PspN4I GGNNCC 7 cut(s) 27, 251, 261, 319, 331, 735, 960
PspPI GGNCC 3 cut(s) 76, 623, 734
PspXI VCTCGAGB 1 cut(s) 158
PsuI RGATCY 4 cut(s) 25, 249, 595, 958
RsaI GTAC 1 cut(s) 578
RsaNI GTAC 1 cut(s) 577
RseI CAYNNNNRTG 2 cut(s) 287, 414
SacI GAGCTC 3 cut(s) 222, 375, 882
SalI GTCGAC 1 cut(s) 293
SaqAI TTAA 3 cut(s) 363, 885, 921
SatI GCNGC 4 cut(s) 133, 263, 681, 1122
Sau3AI GATC 7 cut(s) 8, 25, 121, 249, 595, 958, 988
Sau96I GGNCC 3 cut(s) 76, 623, 734
SchI GAGTC 1 cut(s) 443
ScrFI CCNGG 5 cut(s) 104, 176, 233, 630, 978
SduI GDGCHC 4 cut(s) 222, 320, 375, 882
SfaNI GCATC 2 cut(s) 799, 823
SfcI CTRYAG 1 cut(s) 127
Sfr274I CTCGAG 1 cut(s) 158
SinI GGWCC 3 cut(s) 76, 623, 734
SlaI CTCGAG 1 cut(s) 158
SmiMI CAYNNNNRTG 2 cut(s) 287, 414
SmlI CTYRAG 5 cut(s) 137, 158, 377, 451, 1066
SmoI CTYRAG 5 cut(s) 137, 158, 377, 451, 1066
SphI GCATGC 1 cut(s) 1163
Sse9I AATT 4 cut(s) 278, 334, 707, 1250
SseBI AGGCCT 1 cut(s) 107
SsiI CCGC 5 cut(s) 133, 226, 263, 406, 1053
SstI GAGCTC 3 cut(s) 222, 375, 882
StuI AGGCCT 1 cut(s) 107
StyD4I CCNGG 5 cut(s) 102, 174, 231, 628, 976
StyI CCWWGG 3 cut(s) 15, 768, 954
TaaI ACNGT 1 cut(s) 913
TaiI ACGT 3 cut(s) 432, 607, 1227
TaqI TCGA 3 cut(s) 159, 168, 294
TasI AATT 4 cut(s) 278, 334, 707, 1250
TatI WGTACW 1 cut(s) 576
TauI GCSGC 2 cut(s) 135, 265
TfiI GAWTC 3 cut(s) 205, 349, 867
Tru1I TTAA 3 cut(s) 363, 885, 921
Tru9I TTAA 3 cut(s) 363, 885, 921
TscAI CASTG 4 cut(s) 331, 918, 1123, 1246
TseFI GTSAC 2 cut(s) 490, 515
TseI GCWGC 2 cut(s) 680, 1121
Tsp45I GTSAC 2 cut(s) 490, 515
TspDTI ATGAA 4 cut(s) 154, 271, 676, 1076
TspGWI ACGGA 3 cut(s) 382, 527, 749
TspRI CASTG 4 cut(s) 331, 918, 1123, 1246
VpaK11BI GGWCC 3 cut(s) 76, 623, 734
VspI ATTAAT 1 cut(s) 363
XapI RAATTY 2 cut(s) 707, 1250
XceI RCATGY 1 cut(s) 1163
XcmI CCANNNNNNNNNTGG 1 cut(s) 421
XhoI CTCGAG 1 cut(s) 158
XmiI GTMKAC 2 cut(s) 294, 1080
XmnI GAANNNNTTC 1 cut(s) 711
Zsp2I ATGCAT 1 cut(s) 1161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.