RchiOBHm_Chr5g0039711

26S protease regulatory subunit 6B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002106
Physical Location & Seq
Reverse (-)
34408245 .. 34410015
1771 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31827

Sequence Viewer

Length: 735 bp
ATGCAGCCTACTCTTTCTTCCGACCTAAAGCTCACCGACCAGCACCAATCCTCTGACGAGGACGACCTCTATAGCTGCCTCAAGTCCCTGCAGCGCCAACTGGAGTTCATCGACATCCAAGAGGAGTACGTTAGGATTCTCAGCACTATTAATCGAGAGCATCTGAAGCCCTCCACCTGCGTAGCCTTGCATCTCCACTCCAACGCCCTCGTCGACGTTCTGCCGCCGGAGGCCGACTCTAGTATTTCGTTGCTCAGCCAGAGTGAGAAGCCCGACGTCACTTATAACATAAGCCACGATATCGGGGGATGCGACATTCAAAAGCAAGAAATCCGTGAAGCAGTGGAACTTCCACTTACACACCATGACTTATACAAACAGATTGGAATAGATCCTCCACGTGGTGTATTGCTGTATGGCCCACCTGGAACTGGTAAAACCATGCTAGCTAAGGCTGTTGCCAATCATACAACTGCTGCCTTCATTAGAGTTGTTGGTTCAGAATTTGTTCAGAAGTATTTGGGTGAGGGTCCACGAATGGTCCATGATGTTTTCCGTCTCGCCAAGGAGAATGCACCTGCCATCATCTTTATTGATGAGGTGGATGCTATTGCTACTACAAGGTTTGATCCTCAAACTGGAGCTGATAAAAAAGTTCAACAAATTCTTATGAAGCTCCTCAATCAGGTAGTTCCAGGAAGATTTTATATTGAAGAACAGAAATATTTCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005911 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0030054 GO:0030162 GO:0030163 GO:0030312 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055044 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

244

Amino Acids

27.38

Weight (kDa)

5.34

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_16 PF13191 123 - 170 7.2e-06 AAA ATPase domain
AAA PF00004 136 - 229 8.1e-30 ATPase family associated with various cellular activities (AAA)
AAA_2 PF07724 136 - 228 1.3e-06 AAA domain (Cdc48 subfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 285
AarI CACCTGC 2 cut(s) 185, 586
AatII GACGTC 1 cut(s) 279
Acc36I ACCTGC 2 cut(s) 185, 586
AccI GTMKAC 1 cut(s) 213
AciI CCGC 1 cut(s) 224
AclWI GGATC 2 cut(s) 386, 623
AcsI RAATTY 2 cut(s) 503, 663
AcuI CTGAAG 1 cut(s) 185
AcvI CACGTG 1 cut(s) 401
AcyI GRCGYC 1 cut(s) 276
AdeI CACNNNGTG 1 cut(s) 404
AfaI GTAC 1 cut(s) 128
AfiI CCNNNNNNNGG 4 cut(s) 401, 431, 638, 685
AgsI TTSAA 3 cut(s) 320, 659, 713
AjnI CCWGG 2 cut(s) 424, 694
AluBI AGCT 5 cut(s) 31, 75, 449, 644, 676
AluI AGCT 5 cut(s) 31, 75, 449, 644, 676
Alw26I GTCTC 1 cut(s) 563
AlwI GGATC 2 cut(s) 386, 623
AoxI GGCC 2 cut(s) 231, 418
ApeKI GCWGC 4 cut(s) 4, 75, 91, 476
ApoI RAATTY 2 cut(s) 503, 663
AseI ATTAAT 1 cut(s) 150
Asp700I GAANNNNTTC 2 cut(s) 507, 725
AspLEI GCGC 1 cut(s) 96
AspS9I GGNCC 3 cut(s) 419, 530, 541
AsuHPI GGTGA 2 cut(s) 25, 536
AsuNHI GCTAGC 1 cut(s) 445
AvaII GGWCC 2 cut(s) 530, 541
BbrPI CACGTG 1 cut(s) 401
BbvI GCAGC 4 cut(s) 16, 62, 103, 463
BccI CCATC 1 cut(s) 590
BciT130I CCWGG 2 cut(s) 426, 696
BcoDI GTCTC 1 cut(s) 563
BfaI CTAG 2 cut(s) 240, 446
BfmI CTRYAG 2 cut(s) 70, 89
BfoI RGCGCY 1 cut(s) 97
BfuAI ACCTGC 2 cut(s) 185, 586
BisI GCNGC 5 cut(s) 5, 76, 92, 224, 477
BlpI GCTNAGC 1 cut(s) 254
BlsI GCNGC 5 cut(s) 6, 77, 93, 225, 478
Bme1390I CCNGG 2 cut(s) 426, 696
Bme18I GGWCC 2 cut(s) 530, 541
BmgT120I GGNCC 3 cut(s) 419, 530, 541
BmiI GGNNCC 1 cut(s) 531
BmrFI CCNGG 2 cut(s) 426, 696
BmsI GCATC 4 cut(s) 169, 199, 299, 595
BmtI GCTAGC 1 cut(s) 449
BplI GAGNNNNNCTC 2 cut(s) 221, 253
BpmI CTGGAG 2 cut(s) 122, 660
Bpu10I CCTNAGC 1 cut(s) 450
Bpu1102I GCTNAGC 1 cut(s) 254
BpuEI CTTGAG 1 cut(s) 65
BsaAI YACGTR 1 cut(s) 401
BsaHI GRCGYC 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 564
Bsc4I CCNNNNNNNGG 4 cut(s) 401, 431, 638, 685
Bse1I ACTGG 3 cut(s) 105, 436, 643
BseBI CCWGG 2 cut(s) 426, 696
BseDI CCNNGG 1 cut(s) 564
BseGI GGATG 3 cut(s) 114, 314, 610
BseLI CCNNNNNNNGG 4 cut(s) 401, 431, 638, 685
BseMII CTCAG 2 cut(s) 154, 268
BseNI ACTGG 3 cut(s) 105, 436, 643
BseRI GAGGAG 2 cut(s) 137, 668
BseXI GCAGC 4 cut(s) 16, 62, 103, 463
BshFI GGCC 2 cut(s) 233, 420
BsiSI CCGG 1 cut(s) 227
BslFI GGGAC 1 cut(s) 70
BslI CCNNNNNNNGG 4 cut(s) 401, 431, 638, 685
BsmAI GTCTC 1 cut(s) 563
BsmBI CGTCTC 1 cut(s) 563
BsmFI GGGAC 1 cut(s) 70
BsmI GAATGC 1 cut(s) 577
BsnI GGCC 2 cut(s) 233, 420
Bsp143I GATC 2 cut(s) 391, 628
Bsp1720I GCTNAGC 1 cut(s) 254
BspACI CCGC 1 cut(s) 224
BspANI GGCC 2 cut(s) 233, 420
BspCNI CTCAG 2 cut(s) 153, 267
BspLI GGNNCC 1 cut(s) 531
BspMAI CTGCAG 1 cut(s) 93
BspMI ACCTGC 2 cut(s) 185, 586
BspOI GCTAGC 1 cut(s) 449
BspPI GGATC 2 cut(s) 386, 623
BsrI ACTGG 3 cut(s) 105, 436, 643
BssECI CCNNGG 1 cut(s) 564
BssMI GATC 2 cut(s) 391, 628
BssNI GRCGYC 1 cut(s) 276
BssT1I CCWWGG 1 cut(s) 564
Bst2UI CCWGG 2 cut(s) 426, 696
BstACI GRCGYC 1 cut(s) 276
BstBAI YACGTR 1 cut(s) 401
BstC8I GCNNGC 1 cut(s) 447
BstDEI CTNAG 3 cut(s) 140, 254, 450
BstENI CCTNNNNNAGG 1 cut(s) 683
BstF5I GGATG 3 cut(s) 114, 314, 610
BstH2I RGCGCY 1 cut(s) 97
BstHHI GCGC 1 cut(s) 96
BstKTI GATC 2 cut(s) 394, 631
BstMAI GTCTC 1 cut(s) 563
BstMBI GATC 2 cut(s) 391, 628
BstMWI GCNNNNNNNGC 1 cut(s) 166
BstNI CCWGG 2 cut(s) 426, 696
BstSCI CCNGG 2 cut(s) 424, 694
BstSFI CTRYAG 2 cut(s) 70, 89
BstV1I GCAGC 4 cut(s) 16, 62, 103, 463
BstX2I RGATCY 1 cut(s) 391
BstYI RGATCY 1 cut(s) 391
BsuRI GGCC 2 cut(s) 233, 420
BtsCI GGATG 3 cut(s) 114, 314, 610
BtsI GCAGTG 1 cut(s) 348
BtsIMutI CAGTG 1 cut(s) 348
BveI ACCTGC 2 cut(s) 185, 586
Cac8I GCNNGC 1 cut(s) 447
CfoI GCGC 1 cut(s) 96
Cfr13I GGNCC 3 cut(s) 419, 530, 541
Csp6I GTAC 1 cut(s) 127
CviAII CATG 3 cut(s) 365, 442, 545
CviQI GTAC 1 cut(s) 127
DdeI CTNAG 3 cut(s) 140, 254, 450
DpnI GATC 2 cut(s) 393, 630
DpnII GATC 2 cut(s) 391, 628
DraIII CACNNNGTG 1 cut(s) 404
Eco130I CCWWGG 1 cut(s) 564
Eco32I GATATC 1 cut(s) 301
Eco47I GGWCC 2 cut(s) 530, 541
Eco57I CTGAAG 1 cut(s) 185
Eco72I CACGTG 1 cut(s) 401
EcoNI CCTNNNNNAGG 1 cut(s) 683
EcoRII CCWGG 2 cut(s) 424, 694
EcoRV GATATC 1 cut(s) 301
EcoT14I CCWWGG 1 cut(s) 564
ErhI CCWWGG 1 cut(s) 564
Esp3I CGTCTC 1 cut(s) 563
FaeI CATG 3 cut(s) 368, 445, 548
FaqI GGGAC 1 cut(s) 70
FatI CATG 3 cut(s) 364, 441, 544
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 5 cut(s) 5, 76, 92, 224, 477
FokI GGATG 3 cut(s) 101, 321, 617
Fsp4HI GCNGC 5 cut(s) 5, 76, 92, 224, 477
FspBI CTAG 2 cut(s) 240, 446
GlaI GCGC 1 cut(s) 95
GluI GCNGC 5 cut(s) 5, 76, 92, 224, 477
GsuI CTGGAG 2 cut(s) 122, 660
HaeII RGCGCY 1 cut(s) 97
HaeIII GGCC 2 cut(s) 233, 420
HapII CCGG 1 cut(s) 227
HhaI GCGC 1 cut(s) 96
Hin1I GRCGYC 1 cut(s) 276
Hin1II CATG 3 cut(s) 368, 445, 548
Hin6I GCGC 1 cut(s) 94
HinP1I GCGC 1 cut(s) 94
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HinfI GANTC 2 cut(s) 136, 236
HpaII CCGG 1 cut(s) 227
HphI GGTGA 2 cut(s) 25, 536
Hpy166II GTNNAC 2 cut(s) 214, 533
Hpy188I TCNGA 5 cut(s) 22, 55, 165, 502, 513
Hpy188III TCNNGA 1 cut(s) 155
Hpy8I GTNNAC 2 cut(s) 214, 533
Hpy99I CGWCG 3 cut(s) 215, 218, 278
HpyAV CCTTC 1 cut(s) 490
HpyCH4IV ACGT 4 cut(s) 129, 216, 276, 400
HpyCH4V TGCA 4 cut(s) 4, 91, 190, 575
HpyF10VI GCNNNNNNNGC 1 cut(s) 166
HpyF3I CTNAG 3 cut(s) 140, 254, 450
HpySE526I ACGT 4 cut(s) 129, 216, 276, 400
Hsp92I GRCGYC 1 cut(s) 276
Hsp92II CATG 3 cut(s) 368, 445, 548
HspAI GCGC 1 cut(s) 94
Kzo9I GATC 2 cut(s) 391, 628
LmnI GCTCC 2 cut(s) 641, 681
Lsp1109I GCAGC 4 cut(s) 16, 62, 103, 463
LweI GCATC 4 cut(s) 169, 199, 299, 595
MaeI CTAG 2 cut(s) 240, 446
MaeII ACGT 4 cut(s) 129, 216, 276, 400
MaeIII GTNAC 1 cut(s) 277
MalI GATC 2 cut(s) 393, 630
MboI GATC 2 cut(s) 391, 628
MboII GAAGA 3 cut(s) 9, 711, 725
MflI RGATCY 1 cut(s) 391
MluCI AATT 2 cut(s) 503, 663
MlyI GAGTC 1 cut(s) 230
MmeI TCCRAC 2 cut(s) 45, 225
MroXI GAANNNNTTC 2 cut(s) 507, 725
MseI TTAA 1 cut(s) 150
MspI CCGG 1 cut(s) 227
MspR9I CCNGG 2 cut(s) 426, 696
Mva1269I GAATGC 1 cut(s) 577
MvaI CCWGG 2 cut(s) 426, 696
MwoI GCNNNNNNNGC 1 cut(s) 166
NdeII GATC 2 cut(s) 391, 628
NheI GCTAGC 1 cut(s) 445
NlaIII CATG 3 cut(s) 368, 445, 548
NlaIV GGNNCC 1 cut(s) 531
NmuCI GTSAC 1 cut(s) 277
PaqCI CACCTGC 2 cut(s) 185, 586
PcsI WCGNNNNNNNCGW 2 cut(s) 210, 309
PctI GAATGC 1 cut(s) 577
PdmI GAANNNNTTC 2 cut(s) 507, 725
PfeI GAWTC 1 cut(s) 136
PfoI TCCNGGA 1 cut(s) 694
PkrI GCNGC 5 cut(s) 6, 77, 93, 225, 478
PleI GAGTC 1 cut(s) 230
PmaCI CACGTG 1 cut(s) 401
PmlI CACGTG 1 cut(s) 401
PpsI GAGTC 1 cut(s) 230
Ppu21I YACGTR 1 cut(s) 401
PshBI ATTAAT 1 cut(s) 150
PsiI TTATAA 1 cut(s) 285
Psp6I CCWGG 2 cut(s) 424, 694
PspCI CACGTG 1 cut(s) 401
PspGI CCWGG 2 cut(s) 424, 694
PspN4I GGNNCC 1 cut(s) 531
PspPI GGNCC 3 cut(s) 419, 530, 541
PstI CTGCAG 1 cut(s) 93
PsuI RGATCY 1 cut(s) 391
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
SalI GTCGAC 1 cut(s) 212
SaqAI TTAA 1 cut(s) 150
SatI GCNGC 5 cut(s) 5, 76, 92, 224, 477
Sau3AI GATC 2 cut(s) 391, 628
Sau96I GGNCC 3 cut(s) 419, 530, 541
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 2 cut(s) 426, 696
SfaNI GCATC 4 cut(s) 169, 199, 299, 595
SfcI CTRYAG 2 cut(s) 70, 89
SgrDI CGTCGACG 1 cut(s) 212
SinI GGWCC 2 cut(s) 530, 541
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
Sse9I AATT 2 cut(s) 503, 663
SsiI CCGC 1 cut(s) 224
SspI AATATT 1 cut(s) 725
SspMI CTAG 2 cut(s) 240, 446
StyD4I CCNGG 2 cut(s) 424, 694
StyI CCWWGG 1 cut(s) 564
TaiI ACGT 4 cut(s) 132, 219, 279, 403
TaqI TCGA 3 cut(s) 111, 154, 213
TasI AATT 2 cut(s) 503, 663
TauI GCSGC 1 cut(s) 226
TfiI GAWTC 1 cut(s) 136
Tru1I TTAA 1 cut(s) 150
Tru9I TTAA 1 cut(s) 150
TscAI CASTG 1 cut(s) 348
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 4 cut(s) 4, 75, 91, 476
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 3 cut(s) 97, 472, 686
TspGWI ACGGA 2 cut(s) 323, 545
TspRI CASTG 1 cut(s) 348
VpaK11BI GGWCC 2 cut(s) 530, 541
VspI ATTAAT 1 cut(s) 150
XagI CCTNNNNNAGG 1 cut(s) 683
XapI RAATTY 2 cut(s) 503, 663
XmiI GTMKAC 1 cut(s) 213
XmnI GAANNNNTTC 2 cut(s) 507, 725
XspI CTAG 2 cut(s) 240, 446
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.