FvH4_7g13400

BON1-associated protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002449
Physical Location & Seq
Reverse (-)
12027767 .. 12028995
1229 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g13400.t1

Sequence Viewer

Length: 546 bp
ATGGCAACTCGATCCATAGAGATCACCGTTCTCTCCGCCGAGAATCTCCAATCCAACCGCAAACCCTTGAAGAAAAACTCCTTCGTCACCGTCCGGACCGACTCCCTCGTCACCAAGGTGTACCGGACGGCCGAGACCGACTGCGAAGGCGGAGGGCCGAAGTGGAATGAGAAGATAGTGATGGAGGTTCCTGCTCACGCTAAGAATCTCATCTTGGAAGTGCAATGCCAGAACGGGGCGAGGCCGATCGGAGCGGCCAGCATTCCGGTGACGGAGTTCACCGGCGGGTGGGTGCCGGAGAGCTACTTGCATTTTCTGAGTTATAGGTTGAGGGATGAGAGAGGTGTGAGGAATGGGATTGTGAATATCTCGGTGAGGATGAAGGTGGAGGAAGGTGGGACGTCGTCGATGTATGGGAGGAAGCTGTCGAGTGGTTGCGCTTCGACGTCGTCACAGGTCAAGTTGGGATATCCGGTGGTGGATAACAGCGCCGTCGCAACTGGGGTTCCTGTGAATTGGAAAAGCTATTCTCGAGTTCAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

182

Amino Acids

19.86

Weight (kDa)

9.43

Isoelectric Point (pI)

33.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 6 - 94 8.3e-08 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 107
AatII GACGTC 2 cut(s) 404, 449
AccB1I GGYRCC 1 cut(s) 292
AccBSI CCGCTC 1 cut(s) 254
AccIII TCCGGA 1 cut(s) 93
AciI CCGC 5 cut(s) 36, 58, 150, 254, 285
AclWI GGATC 1 cut(s) 6
AcoI YGGCCR 2 cut(s) 129, 255
AcyI GRCGYC 2 cut(s) 401, 446
AfaI GTAC 1 cut(s) 122
AfiI CCNNNNNNNGG 2 cut(s) 235, 288
AgsI TTSAA 2 cut(s) 70, 539
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AleI CACNNNNGTG 1 cut(s) 116
AluBI AGCT 3 cut(s) 303, 424, 525
AluI AGCT 3 cut(s) 303, 424, 525
Alw26I GTCTC 1 cut(s) 128
AlwI GGATC 1 cut(s) 6
Ama87I CYCGRG 1 cut(s) 531
Aor13HI TCCGGA 1 cut(s) 93
AoxI GGCC 4 cut(s) 129, 155, 242, 255
AspLEI GCGC 2 cut(s) 440, 491
AspS9I GGNCC 2 cut(s) 96, 155
AsuHPI GGTGA 6 cut(s) 16, 79, 103, 271, 280, 385
AvaI CYCGRG 1 cut(s) 531
AvaII GGWCC 1 cut(s) 96
BanI GGYRCC 1 cut(s) 292
BccI CCATC 1 cut(s) 175
BceAI ACGGC 2 cut(s) 144, 476
BcoDI GTCTC 1 cut(s) 128
BfoI RGCGCY 1 cut(s) 492
BisI GCNGC 1 cut(s) 255
BlsI GCNGC 1 cut(s) 256
Bme18I GGWCC 1 cut(s) 96
BmeT110I CYCGRG 1 cut(s) 531
BmgT120I GGNCC 2 cut(s) 96, 155
BmiI GGNNCC 3 cut(s) 189, 294, 507
BmrI ACTGGG 1 cut(s) 510
BmuI ACTGGG 1 cut(s) 510
BsaHI GRCGYC 2 cut(s) 401, 446
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 1 cut(s) 114
BsaWI WCCGGW 4 cut(s) 93, 123, 265, 472
Bsc4I CCNNNNNNNGG 2 cut(s) 235, 288
Bse118I RCCGGY 1 cut(s) 281
Bse1I ACTGG 1 cut(s) 505
Bse3DI GCAATG 1 cut(s) 230
BseAI TCCGGA 1 cut(s) 93
BseDI CCNNGG 1 cut(s) 114
BseGI GGATG 2 cut(s) 340, 384
BseLI CCNNNNNNNGG 2 cut(s) 235, 288
BseMI GCAATG 1 cut(s) 230
BseMII CTCAG 1 cut(s) 308
BseNI ACTGG 1 cut(s) 505
BseX3I CGGCCG 1 cut(s) 129
Bsh1285I CGRYCG 2 cut(s) 132, 249
BshFI GGCC 4 cut(s) 131, 157, 244, 257
BshNI GGYRCC 1 cut(s) 292
BsiEI CGRYCG 2 cut(s) 132, 249
BsiHKCI CYCGRG 1 cut(s) 531
BsiSI CCGG 6 cut(s) 94, 124, 266, 282, 296, 473
BslFI GGGAC 1 cut(s) 412
BslI CCNNNNNNNGG 2 cut(s) 235, 288
BsmAI GTCTC 1 cut(s) 128
BsmFI GGGAC 1 cut(s) 412
BsmI GAATGC 1 cut(s) 261
BsnI GGCC 4 cut(s) 131, 157, 244, 257
Bso31I GGTCTC 1 cut(s) 128
BsoBI CYCGRG 1 cut(s) 531
Bsp13I TCCGGA 1 cut(s) 93
Bsp143I GATC 3 cut(s) 11, 21, 246
BspACI CCGC 5 cut(s) 36, 58, 150, 254, 285
BspANI GGCC 4 cut(s) 131, 157, 244, 257
BspCNI CTCAG 1 cut(s) 309
BspEI TCCGGA 1 cut(s) 93
BspLI GGNNCC 3 cut(s) 189, 294, 507
BspPI GGATC 1 cut(s) 6
BspT107I GGYRCC 1 cut(s) 292
BspTNI GGTCTC 1 cut(s) 128
BsrBI CCGCTC 1 cut(s) 254
BsrDI GCAATG 1 cut(s) 230
BsrFI RCCGGY 1 cut(s) 281
BsrI ACTGG 1 cut(s) 505
BssAI RCCGGY 1 cut(s) 281
BssECI CCNNGG 1 cut(s) 114
BssMI GATC 3 cut(s) 11, 21, 246
BssNI GRCGYC 2 cut(s) 401, 446
BssT1I CCWWGG 1 cut(s) 114
Bst4CI ACNGT 2 cut(s) 28, 91
BstACI GRCGYC 2 cut(s) 401, 446
BstC8I GCNNGC 1 cut(s) 259
BstDEI CTNAG 2 cut(s) 201, 317
BstF5I GGATG 2 cut(s) 340, 384
BstH2I RGCGCY 1 cut(s) 492
BstHHI GCGC 2 cut(s) 440, 491
BstKTI GATC 3 cut(s) 14, 24, 249
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 3 cut(s) 11, 21, 246
BstMCI CGRYCG 2 cut(s) 132, 249
BstZI CGGCCG 1 cut(s) 129
BsuRI GGCC 4 cut(s) 131, 157, 244, 257
BtsCI GGATG 2 cut(s) 340, 384
Cac8I GCNNGC 1 cut(s) 259
CfoI GCGC 2 cut(s) 440, 491
Cfr10I RCCGGY 1 cut(s) 281
Cfr13I GGNCC 2 cut(s) 96, 155
CpoI CGGWCCG 1 cut(s) 96
Csp6I GTAC 1 cut(s) 121
CspI CGGWCCG 1 cut(s) 96
CviJI RGCY 7 cut(s) 131, 157, 244, 257, 303, 424, 525
CviKI_1 RGCY 7 cut(s) 131, 157, 244, 257, 303, 424, 525
CviQI GTAC 1 cut(s) 121
DdeI CTNAG 2 cut(s) 201, 317
DpnI GATC 3 cut(s) 13, 23, 248
DpnII GATC 3 cut(s) 11, 21, 246
DrdI GACNNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 107
EaeI YGGCCR 2 cut(s) 129, 255
EagI CGGCCG 1 cut(s) 129
EciI GGCGGA 2 cut(s) 25, 165
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 114
Eco31I GGTCTC 1 cut(s) 128
Eco32I GATATC 1 cut(s) 470
Eco47I GGWCC 1 cut(s) 96
Eco52I CGGCCG 1 cut(s) 129
Eco88I CYCGRG 1 cut(s) 531
EcoRV GATATC 1 cut(s) 470
EcoT14I CCWWGG 1 cut(s) 114
ErhI CCWWGG 1 cut(s) 114
FaiI YATR 3 cut(s) 17, 324, 414
FaqI GGGAC 1 cut(s) 412
FauI CCCGC 1 cut(s) 278
Fnu4HI GCNGC 1 cut(s) 255
FokI GGATG 2 cut(s) 347, 391
Fsp4HI GCNGC 1 cut(s) 255
GlaI GCGC 2 cut(s) 439, 490
GluI GCNGC 1 cut(s) 255
HaeII RGCGCY 1 cut(s) 492
HaeIII GGCC 4 cut(s) 131, 157, 244, 257
HapII CCGG 6 cut(s) 94, 124, 266, 282, 296, 473
HhaI GCGC 2 cut(s) 440, 491
Hin1I GRCGYC 2 cut(s) 401, 446
Hin6I GCGC 2 cut(s) 438, 489
HinP1I GCGC 2 cut(s) 438, 489
HinfI GANTC 3 cut(s) 43, 101, 205
HpaII CCGG 6 cut(s) 94, 124, 266, 282, 296, 473
HphI GGTGA 6 cut(s) 16, 79, 103, 271, 280, 385
Hpy166II GTNNAC 2 cut(s) 121, 279
Hpy188I TCNGA 2 cut(s) 251, 318
Hpy188III TCNNGA 2 cut(s) 94, 531
Hpy8I GTNNAC 2 cut(s) 121, 279
Hpy99I CGWCG 5 cut(s) 406, 409, 448, 451, 497
HpyAV CCTTC 4 cut(s) 91, 140, 376, 386
HpyCH4III ACNGT 2 cut(s) 28, 91
HpyCH4IV ACGT 2 cut(s) 401, 446
HpyCH4V TGCA 2 cut(s) 223, 310
HpyF3I CTNAG 2 cut(s) 201, 317
HpySE526I ACGT 2 cut(s) 401, 446
Hsp92I GRCGYC 2 cut(s) 401, 446
HspAI GCGC 2 cut(s) 438, 489
Kpn2I TCCGGA 1 cut(s) 93
Kzo9I GATC 3 cut(s) 11, 21, 246
LmnI GCTCC 1 cut(s) 251
MaeII ACGT 2 cut(s) 401, 446
MaeIII GTNAC 4 cut(s) 85, 109, 268, 450
MalI GATC 3 cut(s) 13, 23, 248
MbiI CCGCTC 1 cut(s) 254
MboI GATC 3 cut(s) 11, 21, 246
MboII GAAGA 2 cut(s) 82, 184
MluCI AATT 1 cut(s) 514
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 1 cut(s) 78
MroI TCCGGA 1 cut(s) 93
MslI CAYNNNNRTG 2 cut(s) 116, 266
MspI CCGG 6 cut(s) 94, 124, 266, 282, 296, 473
Mva1269I GAATGC 1 cut(s) 261
NdeII GATC 3 cut(s) 11, 21, 246
NlaIV GGNNCC 3 cut(s) 189, 294, 507
NmeAIII GCCGAG 2 cut(s) 64, 157
NmuCI GTSAC 4 cut(s) 85, 109, 268, 450
OliI CACNNNNGTG 1 cut(s) 116
PaeR7I CTCGAG 1 cut(s) 531
PctI GAATGC 1 cut(s) 261
PfeI GAWTC 2 cut(s) 43, 205
PflFI GACNNNGTC 2 cut(s) 403, 448
PkrI GCNGC 1 cut(s) 256
Ple19I CGATCG 1 cut(s) 249
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
PspN4I GGNNCC 3 cut(s) 189, 294, 507
PspPI GGNCC 2 cut(s) 96, 155
PsyI GACNNNGTC 2 cut(s) 403, 448
PvuI CGATCG 1 cut(s) 249
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
RseI CAYNNNNRTG 2 cut(s) 116, 266
Rsr2I CGGWCCG 1 cut(s) 96
RsrII CGGWCCG 1 cut(s) 96
SatI GCNGC 1 cut(s) 255
Sau3AI GATC 3 cut(s) 11, 21, 246
Sau96I GGNCC 2 cut(s) 96, 155
SchI GAGTC 1 cut(s) 95
Sfr274I CTCGAG 1 cut(s) 531
SgrAI CRCCGGYG 1 cut(s) 281
SinI GGWCC 1 cut(s) 96
SlaI CTCGAG 1 cut(s) 531
SmiMI CAYNNNNRTG 2 cut(s) 116, 266
SmlI CTYRAG 1 cut(s) 531
SmoI CTYRAG 1 cut(s) 531
Sse9I AATT 1 cut(s) 514
SsiI CCGC 5 cut(s) 36, 58, 150, 254, 285
SspI AATATT 1 cut(s) 542
StyI CCWWGG 1 cut(s) 114
TaaI ACNGT 2 cut(s) 28, 91
TaiI ACGT 2 cut(s) 404, 449
TaqI TCGA 5 cut(s) 10, 407, 428, 443, 532
TaqII GACCGA 2 cut(s) 113, 152
TasI AATT 1 cut(s) 514
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 2 cut(s) 43, 205
TseFI GTSAC 4 cut(s) 85, 109, 268, 450
Tsp45I GTSAC 4 cut(s) 85, 109, 268, 450
TspDTI ATGAA 1 cut(s) 395
TspGWI ACGGA 1 cut(s) 287
Tth111I GACNNNGTC 2 cut(s) 403, 448
VpaK11BI GGWCC 1 cut(s) 96
XhoI CTCGAG 1 cut(s) 531
ZraI GACGTC 2 cut(s) 402, 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.