MD07G1126500.v1.1

BON1-associated protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002449
Physical Location & Seq
Forward (+)
17858945 .. 17859481
537 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1126500.v1.1.491

Sequence Viewer

Length: 537 bp
ATGGCAGCCAATTCCCGGACGCTTGAAATCAAGGTGATCTCCGCCGAGAACCTGAAATTAGATCGAAAATCCATCAAGAAAAACGCCTCCGTGACCGTCCGGACCGACACCAACAGCCAGTTTTGCACTACGGACATTGACACCGAAGGCGGCGCCCACCCACGGTGGAACGAGAAGCTCGTGCTCGACTTGCCAACGCACTCGAAGTCCATCACAGTGGAGGTCCATTGCAAGACTTCGTCCGGCGTCAGGACGATCGGGACGGCAACGGTTCCGGCTTCTGATTTTGTTGGCGGGTACGTACCGGAGGGTTACCTGCATTTTTTGAGTTACAGGCTGAGGAATCACAAGGGGGAGAGGAATGGGATTATTAATATTTCTGTAAGAATGAAGGTTCCGGAGATGTACGCTTGTGCAAGTTCAACGACGTGGTCTCACTCAACGATGGGGTTTCCGGCGGCTGGCAACAAAAGTTTCGGCGGCGGCGTTGTGACAGGAGTTCCGGTTTGGTACGGTTCCTATCAGAAAAATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

179

Amino Acids

19.46

Weight (kDa)

9.4

Isoelectric Point (pI)

32.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 7 - 103 1.9e-11 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 324
AccB1I GGYRCC 1 cut(s) 152
AccIII TCCGGA 2 cut(s) 99, 397
AciI CCGC 6 cut(s) 42, 150, 294, 458, 480, 483
AcyI GRCGYC 2 cut(s) 153, 246
AfaI GTAC 4 cut(s) 299, 303, 407, 512
AfiI CCNNNNNNNGG 4 cut(s) 15, 162, 249, 461
AgsI TTSAA 2 cut(s) 26, 423
AjiI CACGTC 1 cut(s) 429
AluBI AGCT 1 cut(s) 178
AluI AGCT 1 cut(s) 178
Alw21I GWGCWC 1 cut(s) 186
Alw26I GTCTC 1 cut(s) 438
Aor13HI TCCGGA 2 cut(s) 99, 397
ApeKI GCWGC 1 cut(s) 5
AseI ATTAAT 1 cut(s) 372
AspLEI GCGC 1 cut(s) 155
AspS9I GGNCC 2 cut(s) 102, 223
AsuC2I CCSGG 1 cut(s) 16
AsuHPI GGTGA 1 cut(s) 46
AvaII GGWCC 2 cut(s) 102, 223
BanI GGYRCC 1 cut(s) 152
BauI CACGAG 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 186
BbvCI CCTCAGC 1 cut(s) 338
BbvI GCAGC 1 cut(s) 17
BccI CCATC 3 cut(s) 80, 218, 439
BceAI ACGGC 1 cut(s) 279
BcnI CCSGG 1 cut(s) 16
BcoDI GTCTC 1 cut(s) 438
BfoI RGCGCY 1 cut(s) 156
BfuAI ACCTGC 1 cut(s) 324
BisI GCNGC 5 cut(s) 6, 151, 459, 481, 484
BlsI GCNGC 5 cut(s) 7, 152, 460, 482, 485
Bme1390I CCNGG 1 cut(s) 16
Bme18I GGWCC 2 cut(s) 102, 223
BmgBI CACGTC 1 cut(s) 429
BmgT120I GGNCC 2 cut(s) 102, 223
BmiI GGNNCC 4 cut(s) 154, 273, 396, 517
BmrFI CCNGG 1 cut(s) 16
Bpu10I CCTNAGC 1 cut(s) 338
BpuMI CCSGG 1 cut(s) 16
BsaAI YACGTR 1 cut(s) 301
BsaHI GRCGYC 2 cut(s) 153, 246
BsaI GGTCTC 1 cut(s) 438
BsaJI CCNNGG 1 cut(s) 161
BsaWI WCCGGW 4 cut(s) 99, 304, 397, 502
BsaXI ACNNNNNCTCC 2 cut(s) 489, 519
Bsc4I CCNNNNNNNGG 4 cut(s) 15, 162, 249, 461
Bse1I ACTGG 1 cut(s) 118
Bse3DI GCAATG 1 cut(s) 226
BseAI TCCGGA 2 cut(s) 99, 397
BseDI CCNNGG 1 cut(s) 161
BseLI CCNNNNNNNGG 4 cut(s) 15, 162, 249, 461
BseMI GCAATG 1 cut(s) 226
BseMII CTCAG 1 cut(s) 329
BseNI ACTGG 1 cut(s) 118
BseXI GCAGC 1 cut(s) 17
Bsh1285I CGRYCG 1 cut(s) 258
BshNI GGYRCC 1 cut(s) 152
BsiEI CGRYCG 1 cut(s) 258
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 8 cut(s) 16, 100, 243, 275, 305, 398, 455, 503
BslFI GGGAC 1 cut(s) 274
BslI CCNNNNNNNGG 4 cut(s) 15, 162, 249, 461
BsmAI GTCTC 1 cut(s) 438
BsmFI GGGAC 1 cut(s) 274
Bso31I GGTCTC 1 cut(s) 438
Bsp1286I GDGCHC 1 cut(s) 186
Bsp13I TCCGGA 2 cut(s) 99, 397
Bsp143I GATC 3 cut(s) 36, 61, 255
BspACI CCGC 6 cut(s) 42, 150, 294, 458, 480, 483
BspCNI CTCAG 1 cut(s) 330
BspEI TCCGGA 2 cut(s) 99, 397
BspLI GGNNCC 4 cut(s) 154, 273, 396, 517
BspMI ACCTGC 1 cut(s) 324
BspT107I GGYRCC 1 cut(s) 152
BspTNI GGTCTC 1 cut(s) 438
BsrDI GCAATG 1 cut(s) 226
BsrI ACTGG 1 cut(s) 118
BssECI CCNNGG 1 cut(s) 161
BssMI GATC 3 cut(s) 36, 61, 255
BssNI GRCGYC 2 cut(s) 153, 246
BssSI CACGAG 1 cut(s) 179
Bst2BI CACGAG 1 cut(s) 179
Bst4CI ACNGT 5 cut(s) 97, 165, 217, 271, 515
BstACI GRCGYC 2 cut(s) 153, 246
BstBAI YACGTR 1 cut(s) 301
BstC8I GCNNGC 1 cut(s) 463
BstDEI CTNAG 1 cut(s) 338
BstDSI CCRYGG 1 cut(s) 161
BstEII GGTNACC 1 cut(s) 311
BstH2I RGCGCY 1 cut(s) 156
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 3 cut(s) 39, 64, 258
BstMAI GTCTC 1 cut(s) 438
BstMBI GATC 3 cut(s) 36, 61, 255
BstMCI CGRYCG 1 cut(s) 258
BstMWI GCNNNNNNNGC 2 cut(s) 123, 190
BstPI GGTNACC 1 cut(s) 311
BstSCI CCNGG 1 cut(s) 14
BstSNI TACGTA 1 cut(s) 301
BstV1I GCAGC 1 cut(s) 17
BstXI CCANNNNNNTGG 1 cut(s) 217
BtgI CCRYGG 1 cut(s) 161
BtrI CACGTC 1 cut(s) 429
BtsIMutI CAGTG 1 cut(s) 222
BveI ACCTGC 1 cut(s) 324
Cac8I GCNNGC 1 cut(s) 463
CfoI GCGC 1 cut(s) 155
Cfr13I GGNCC 2 cut(s) 102, 223
CpoI CGGWCCG 1 cut(s) 102
CseI GACGC 2 cut(s) 28, 235
Csp6I GTAC 4 cut(s) 298, 302, 406, 511
CspI CGGWCCG 1 cut(s) 102
CviJI RGCY 6 cut(s) 8, 117, 178, 278, 337, 461
CviKI_1 RGCY 6 cut(s) 8, 117, 178, 278, 337, 461
CviQI GTAC 4 cut(s) 298, 302, 406, 511
DdeI CTNAG 1 cut(s) 338
DinI GGCGCC 1 cut(s) 154
DpnI GATC 3 cut(s) 38, 63, 257
DpnII GATC 3 cut(s) 36, 61, 255
EciI GGCGGA 1 cut(s) 31
Eco105I TACGTA 1 cut(s) 301
Eco31I GGTCTC 1 cut(s) 438
Eco47I GGWCC 2 cut(s) 102, 223
Eco91I GGTNACC 1 cut(s) 311
EcoO65I GGTNACC 1 cut(s) 311
EgeI GGCGCC 1 cut(s) 154
EheI GGCGCC 1 cut(s) 154
FaqI GGGAC 1 cut(s) 274
FauI CCCGC 1 cut(s) 287
Fnu4HI GCNGC 5 cut(s) 6, 151, 459, 481, 484
Fsp4HI GCNGC 5 cut(s) 6, 151, 459, 481, 484
GlaI GCGC 1 cut(s) 154
GluI GCNGC 5 cut(s) 6, 151, 459, 481, 484
HaeII RGCGCY 1 cut(s) 156
HapII CCGG 8 cut(s) 16, 100, 243, 275, 305, 398, 455, 503
HgaI GACGC 2 cut(s) 28, 235
HhaI GCGC 1 cut(s) 155
Hin1I GRCGYC 2 cut(s) 153, 246
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HinfI GANTC 1 cut(s) 343
HpaII CCGG 8 cut(s) 16, 100, 243, 275, 305, 398, 455, 503
HphI GGTGA 1 cut(s) 46
Hpy188I TCNGA 2 cut(s) 283, 525
Hpy188III TCNNGA 5 cut(s) 76, 100, 250, 259, 398
Hpy99I CGWCG 1 cut(s) 430
HpyAV CCTTC 2 cut(s) 140, 385
HpyCH4III ACNGT 5 cut(s) 97, 165, 217, 271, 515
HpyCH4IV ACGT 2 cut(s) 300, 428
HpyCH4V TGCA 4 cut(s) 126, 231, 319, 416
HpyF10VI GCNNNNNNNGC 2 cut(s) 123, 190
HpyF3I CTNAG 1 cut(s) 338
HpySE526I ACGT 2 cut(s) 300, 428
Hsp92I GRCGYC 2 cut(s) 153, 246
HspAI GCGC 1 cut(s) 153
KasI GGCGCC 1 cut(s) 152
Kpn2I TCCGGA 2 cut(s) 99, 397
Kzo9I GATC 3 cut(s) 36, 61, 255
Lsp1109I GCAGC 1 cut(s) 17
MaeII ACGT 2 cut(s) 300, 428
MaeIII GTNAC 4 cut(s) 91, 311, 329, 490
MalI GATC 3 cut(s) 38, 63, 257
MboI GATC 3 cut(s) 36, 61, 255
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 3 cut(s) 10, 56, 529
Mly113I GGCGCC 1 cut(s) 153
MnlI CCTC 5 cut(s) 97, 214, 301, 333, 351
MroI TCCGGA 2 cut(s) 99, 397
MseI TTAA 1 cut(s) 372
MslI CAYNNNNRTG 1 cut(s) 215
MspI CCGG 8 cut(s) 16, 100, 243, 275, 305, 398, 455, 503
MspR9I CCNGG 1 cut(s) 16
MwoI GCNNNNNNNGC 2 cut(s) 123, 190
NarI GGCGCC 1 cut(s) 153
NciI CCSGG 1 cut(s) 16
NdeII GATC 3 cut(s) 36, 61, 255
NlaIV GGNNCC 4 cut(s) 154, 273, 396, 517
NmeAIII GCCGAG 1 cut(s) 70
NmuCI GTSAC 2 cut(s) 91, 490
PcsI WCGNNNNNNNCGW 2 cut(s) 177, 483
PfeI GAWTC 1 cut(s) 343
PflFI GACNNNGTC 2 cut(s) 238, 430
PfoI TCCNGGA 1 cut(s) 14
PkrI GCNGC 5 cut(s) 7, 152, 460, 482, 485
Ple19I CGATCG 1 cut(s) 258
PluTI GGCGCC 1 cut(s) 156
Ppu21I YACGTR 1 cut(s) 301
PshBI ATTAAT 1 cut(s) 372
PspEI GGTNACC 1 cut(s) 311
PspN4I GGNNCC 4 cut(s) 154, 273, 396, 517
PspPI GGNCC 2 cut(s) 102, 223
PsyI GACNNNGTC 2 cut(s) 238, 430
PvuI CGATCG 1 cut(s) 258
RsaI GTAC 4 cut(s) 299, 303, 407, 512
RsaNI GTAC 4 cut(s) 298, 302, 406, 511
RseI CAYNNNNRTG 1 cut(s) 215
Rsr2I CGGWCCG 1 cut(s) 102
RsrII CGGWCCG 1 cut(s) 102
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 5 cut(s) 6, 151, 459, 481, 484
Sau3AI GATC 3 cut(s) 36, 61, 255
Sau96I GGNCC 2 cut(s) 102, 223
ScrFI CCNGG 1 cut(s) 16
SduI GDGCHC 1 cut(s) 186
SetI ASST 8 cut(s) 36, 54, 180, 225, 303, 318, 396, 431
SfoI GGCGCC 1 cut(s) 154
SinI GGWCC 2 cut(s) 102, 223
SmiMI CAYNNNNRTG 1 cut(s) 215
SnaBI TACGTA 1 cut(s) 301
Sse9I AATT 3 cut(s) 10, 56, 529
SsiI CCGC 6 cut(s) 42, 150, 294, 458, 480, 483
SspDI GGCGCC 1 cut(s) 152
SspI AATATT 1 cut(s) 376
StyD4I CCNGG 1 cut(s) 14
TaaI ACNGT 5 cut(s) 97, 165, 217, 271, 515
TaiI ACGT 2 cut(s) 303, 431
TaqI TCGA 3 cut(s) 64, 186, 203
TaqII GACCGA 1 cut(s) 119
TasI AATT 3 cut(s) 10, 56, 529
TauI GCSGC 4 cut(s) 153, 461, 483, 486
TfiI GAWTC 1 cut(s) 343
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TscAI CASTG 1 cut(s) 222
TseFI GTSAC 2 cut(s) 91, 490
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 2 cut(s) 91, 490
TspDTI ATGAA 1 cut(s) 404
TspGWI ACGGA 2 cut(s) 79, 146
TspRI CASTG 1 cut(s) 222
Tth111I GACNNNGTC 2 cut(s) 238, 430
VpaK11BI GGWCC 2 cut(s) 102, 223
VspI ATTAAT 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.