pycom01g07440

BON1-associated protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0002449
Physical Location & Seq
Forward (+)
8364774 .. 8365634
861 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g07440.2

Sequence Viewer

Length: 819 bp
ATGAATTACTTGGAAAAGTTTCTTTGTGTATGTAAGAGGCATGACGTAATTATAAAAGGCGCAAAGTCAACTAAAAAAACCAAATTACACTTGTCCCCATTGCCGCGCGAGTTTATTTCAGAGTCTAATTTTCAATTCCACAGAAGAAACCACGTGGCTGTTGCTCTCTCCTTTCTCACACACTTCCCTCAACACTCCGTTCCTATATATAACCCCACCCGCACCTATTTCCACTCAACGGCTCTTACAAGTCCCCTAGCACAACCAATTCATCCACCATGGCAGCCAATTCCCGGACGCTCGAGATCGACGGTGATCTCCGCCGAGAATCTGAAATTAGATCGAAAACCCATCAAGAAAAACTCCTCTGTGACCGTCCGGCCCGACACCAACAGTCAGTTTTGCACCACGGAAATGGACACCGAAGGCGGCGCCTACCCTCGATGGAACGAGAAGCTCGTGCTCGACTTGCCAGCGCACTCGAGGTCCATCACGGTAGATGTCCAATGCAAGACGTCGTACGGCGTCAGGACGGTCGGGACGGCGACGATTCCGTCGTCGGATTTTGTAGGTGGGTACGTGCCGGAGGGTTACCTGCATTTTTTGAGTTACAGGCTGAGGGATAACAGAGGGGAGAGGAATGGGATTATTAATATTTCTGTGAGAATGAAGGTTCCGGAGATTTACGCTTGTTCAAGTTCAACGACGTCGTCTCACTCGAGAATGGGGTTTCCGGTGGCTGAGAACAATAATTTTGGTGGCGGCGTTGCGACAGGAGTTCCGGTTTGGTATGGTTCTTATCAGAAAAAACATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

273

Amino Acids

30.6

Weight (kDa)

9.72

Isoelectric Point (pI)

43.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 53
AasI GACNNNNNNGTC 1 cut(s) 553
AatII GACGTC 2 cut(s) 518, 710
Acc36I ACCTGC 1 cut(s) 603
AccB1I GGYRCC 1 cut(s) 431
AccII CGCG 2 cut(s) 106, 108
AccIII TCCGGA 1 cut(s) 676
AciI CCGC 5 cut(s) 104, 220, 321, 429, 762
AcvI CACGTG 1 cut(s) 154
AcyI GRCGYC 4 cut(s) 432, 515, 525, 707
AfaI GTAC 2 cut(s) 521, 578
AfiI CCNNNNNNNGG 2 cut(s) 238, 293
AgsI TTSAA 3 cut(s) 134, 696, 702
AluBI AGCT 1 cut(s) 457
AluI AGCT 1 cut(s) 457
Alw21I GWGCWC 1 cut(s) 465
Alw26I GTCTC 1 cut(s) 717
Ama87I CYCGRG 3 cut(s) 301, 481, 718
Aor13HI TCCGGA 1 cut(s) 676
AoxI GGCC 1 cut(s) 380
ApeKI GCWGC 1 cut(s) 283
AseI ATTAAT 1 cut(s) 651
Asp700I GAANNNNTTC 1 cut(s) 18
AspLEI GCGC 4 cut(s) 62, 108, 434, 478
AspS9I GGNCC 2 cut(s) 381, 486
AsuC2I CCSGG 1 cut(s) 294
AsuHPI GGTGA 1 cut(s) 325
AvaI CYCGRG 3 cut(s) 301, 481, 718
AvaII GGWCC 1 cut(s) 486
BanI GGYRCC 1 cut(s) 431
BauI CACGAG 1 cut(s) 458
BbrPI CACGTG 1 cut(s) 154
Bbv12I GWGCWC 1 cut(s) 465
BbvCI CCTCAGC 1 cut(s) 617
BbvI GCAGC 1 cut(s) 295
BccI CCATC 3 cut(s) 359, 438, 497
BceAI ACGGC 3 cut(s) 255, 538, 558
BcnI CCSGG 1 cut(s) 294
BcoDI GTCTC 1 cut(s) 717
BfaI CTAG 1 cut(s) 257
BfoI RGCGCY 1 cut(s) 435
BfuAI ACCTGC 1 cut(s) 603
BisI GCNGC 4 cut(s) 104, 284, 430, 763
BlsI GCNGC 4 cut(s) 105, 285, 431, 764
Bme1390I CCNGG 1 cut(s) 294
Bme18I GGWCC 1 cut(s) 486
BmeT110I CYCGRG 3 cut(s) 301, 481, 718
BmgT120I GGNCC 2 cut(s) 381, 486
BmiI GGNNCC 2 cut(s) 433, 675
BmrFI CCNGG 1 cut(s) 294
Bpu10I CCTNAGC 1 cut(s) 617
BpuMI CCSGG 1 cut(s) 294
BsaAI YACGTR 2 cut(s) 154, 580
BsaHI GRCGYC 4 cut(s) 432, 515, 525, 707
BsaJI CCNNGG 2 cut(s) 278, 408
BsaWI WCCGGW 3 cut(s) 676, 733, 781
BsaXI ACNNNNNCTCC 2 cut(s) 768, 798
Bsc4I CCNNNNNNNGG 2 cut(s) 238, 293
Bse3DI GCAATG 1 cut(s) 98
BseAI TCCGGA 1 cut(s) 676
BseDI CCNNGG 2 cut(s) 278, 408
BseGI GGATG 1 cut(s) 271
BseLI CCNNNNNNNGG 2 cut(s) 238, 293
BseMI GCAATG 1 cut(s) 98
BseMII CTCAG 2 cut(s) 608, 732
BseRI GAGGAG 1 cut(s) 355
BseXI GCAGC 1 cut(s) 295
Bsh1236I CGCG 2 cut(s) 106, 108
Bsh1285I CGRYCG 1 cut(s) 537
BshFI GGCC 1 cut(s) 382
BshNI GGYRCC 1 cut(s) 431
BsiEI CGRYCG 1 cut(s) 537
BsiHKAI GWGCWC 1 cut(s) 465
BsiHKCI CYCGRG 3 cut(s) 301, 481, 718
BsiSI CCGG 6 cut(s) 294, 379, 584, 677, 734, 782
BsiWI CGTACG 1 cut(s) 519
BslFI GGGAC 3 cut(s) 79, 237, 553
BslI CCNNNNNNNGG 2 cut(s) 238, 293
BsmAI GTCTC 1 cut(s) 717
BsmBI CGTCTC 1 cut(s) 717
BsmFI GGGAC 3 cut(s) 79, 237, 553
BsnI GGCC 1 cut(s) 382
BsoBI CYCGRG 3 cut(s) 301, 481, 718
Bsp1286I GDGCHC 1 cut(s) 465
Bsp13I TCCGGA 1 cut(s) 676
Bsp143I GATC 3 cut(s) 305, 315, 340
Bsp19I CCATGG 1 cut(s) 278
BspACI CCGC 5 cut(s) 104, 220, 321, 429, 762
BspANI GGCC 1 cut(s) 382
BspCNI CTCAG 2 cut(s) 609, 733
BspEI TCCGGA 1 cut(s) 676
BspFNI CGCG 2 cut(s) 106, 108
BspLI GGNNCC 2 cut(s) 433, 675
BspMI ACCTGC 1 cut(s) 603
BspT107I GGYRCC 1 cut(s) 431
BsrDI GCAATG 1 cut(s) 98
BssECI CCNNGG 2 cut(s) 278, 408
BssMI GATC 3 cut(s) 305, 315, 340
BssNI GRCGYC 4 cut(s) 432, 515, 525, 707
BssSI CACGAG 1 cut(s) 458
BssT1I CCWWGG 1 cut(s) 278
Bst2BI CACGAG 1 cut(s) 458
Bst4CI ACNGT 5 cut(s) 313, 376, 395, 496, 535
BstACI GRCGYC 4 cut(s) 432, 515, 525, 707
BstBAI YACGTR 2 cut(s) 154, 580
BstC8I GCNNGC 1 cut(s) 474
BstDEI CTNAG 2 cut(s) 617, 741
BstDSI CCRYGG 2 cut(s) 278, 408
BstEII GGTNACC 1 cut(s) 590
BstF5I GGATG 1 cut(s) 271
BstFNI CGCG 2 cut(s) 106, 108
BstH2I RGCGCY 1 cut(s) 435
BstHHI GCGC 4 cut(s) 62, 108, 434, 478
BstKTI GATC 3 cut(s) 308, 318, 343
BstMAI GTCTC 1 cut(s) 717
BstMBI GATC 3 cut(s) 305, 315, 340
BstMCI CGRYCG 1 cut(s) 537
BstMWI GCNNNNNNNGC 1 cut(s) 469
BstPI GGTNACC 1 cut(s) 590
BstSCI CCNGG 1 cut(s) 292
BstUI CGCG 2 cut(s) 106, 108
BstV1I GCAGC 1 cut(s) 295
BstXI CCANNNNNNTGG 1 cut(s) 415
BsuRI GGCC 1 cut(s) 382
BtgI CCRYGG 2 cut(s) 278, 408
BtsCI GGATG 1 cut(s) 271
BveI ACCTGC 1 cut(s) 603
Cac8I GCNNGC 1 cut(s) 474
CfoI GCGC 4 cut(s) 62, 108, 434, 478
Cfr13I GGNCC 2 cut(s) 381, 486
CseI GACGC 2 cut(s) 306, 514
Csp6I GTAC 2 cut(s) 520, 577
CviAII CATG 2 cut(s) 41, 279
CviJI RGCY 7 cut(s) 158, 242, 286, 382, 457, 616, 740
CviKI_1 RGCY 7 cut(s) 158, 242, 286, 382, 457, 616, 740
CviQI GTAC 2 cut(s) 520, 577
DdeI CTNAG 2 cut(s) 617, 741
DinI GGCGCC 1 cut(s) 433
DpnI GATC 3 cut(s) 307, 317, 342
DpnII GATC 3 cut(s) 305, 315, 340
DrdI GACNNNNNNGTC 1 cut(s) 553
DseDI GACNNNNNNGTC 1 cut(s) 553
EciI GGCGGA 1 cut(s) 310
Eco130I CCWWGG 1 cut(s) 278
Eco47I GGWCC 1 cut(s) 486
Eco72I CACGTG 1 cut(s) 154
Eco88I CYCGRG 3 cut(s) 301, 481, 718
Eco91I GGTNACC 1 cut(s) 590
EcoO65I GGTNACC 1 cut(s) 590
EcoT14I CCWWGG 1 cut(s) 278
EgeI GGCGCC 1 cut(s) 433
EheI GGCGCC 1 cut(s) 433
ErhI CCWWGG 1 cut(s) 278
Esp3I CGTCTC 1 cut(s) 717
FaeI CATG 2 cut(s) 44, 282
FaiI YATR 8 cut(s) 31, 42, 53, 206, 208, 210, 280, 792
FaqI GGGAC 3 cut(s) 79, 237, 553
FatI CATG 2 cut(s) 40, 278
FauI CCCGC 1 cut(s) 227
Fnu4HI GCNGC 4 cut(s) 104, 284, 430, 763
FokI GGATG 1 cut(s) 258
Fsp4HI GCNGC 4 cut(s) 104, 284, 430, 763
FspBI CTAG 1 cut(s) 257
GlaI GCGC 4 cut(s) 61, 107, 433, 477
GluI GCNGC 4 cut(s) 104, 284, 430, 763
HaeII RGCGCY 1 cut(s) 435
HaeIII GGCC 1 cut(s) 382
HapII CCGG 6 cut(s) 294, 379, 584, 677, 734, 782
HgaI GACGC 2 cut(s) 306, 514
HhaI GCGC 4 cut(s) 62, 108, 434, 478
Hin1I GRCGYC 4 cut(s) 432, 515, 525, 707
Hin1II CATG 2 cut(s) 44, 282
Hin6I GCGC 4 cut(s) 60, 106, 432, 476
HinP1I GCGC 4 cut(s) 60, 106, 432, 476
HincII GTYRAC 1 cut(s) 69
HindII GTYRAC 1 cut(s) 69
HinfI GANTC 3 cut(s) 122, 328, 550
HpaII CCGG 6 cut(s) 294, 379, 584, 677, 734, 782
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 1 cut(s) 69
Hpy188I TCNGA 4 cut(s) 121, 333, 562, 804
Hpy188III TCNNGA 6 cut(s) 303, 355, 529, 538, 677, 720
Hpy8I GTNNAC 1 cut(s) 69
Hpy99I CGWCG 7 cut(s) 313, 520, 550, 559, 562, 709, 712
HpyAV CCTTC 2 cut(s) 419, 664
HpyCH4III ACNGT 5 cut(s) 313, 376, 395, 496, 535
HpyCH4IV ACGT 5 cut(s) 45, 153, 515, 579, 707
HpyCH4V TGCA 3 cut(s) 405, 510, 598
HpyF10VI GCNNNNNNNGC 1 cut(s) 469
HpyF3I CTNAG 2 cut(s) 617, 741
HpySE526I ACGT 5 cut(s) 45, 153, 515, 579, 707
Hsp92I GRCGYC 4 cut(s) 432, 515, 525, 707
Hsp92II CATG 2 cut(s) 44, 282
HspAI GCGC 4 cut(s) 60, 106, 432, 476
KasI GGCGCC 1 cut(s) 431
Kpn2I TCCGGA 1 cut(s) 676
Kzo9I GATC 3 cut(s) 305, 315, 340
Lsp1109I GCAGC 1 cut(s) 295
MaeI CTAG 1 cut(s) 257
MaeII ACGT 5 cut(s) 45, 153, 515, 579, 707
MaeIII GTNAC 3 cut(s) 370, 590, 608
MalI GATC 3 cut(s) 307, 317, 342
MboI GATC 3 cut(s) 305, 315, 340
MboII GAAGA 1 cut(s) 156
MhlI GDGCHC 1 cut(s) 465
MluCI AATT 9 cut(s) 4, 48, 83, 127, 134, 267, 288, 335, 751
Mly113I GGCGCC 1 cut(s) 432
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 1 cut(s) 540
MnlI CCTC 9 cut(s) 30, 198, 376, 450, 477, 580, 612, 623, 630
MroI TCCGGA 1 cut(s) 676
MroXI GAANNNNTTC 1 cut(s) 18
MseI TTAA 1 cut(s) 651
MslI CAYNNNNRTG 1 cut(s) 413
MspI CCGG 6 cut(s) 294, 379, 584, 677, 734, 782
MspR9I CCNGG 1 cut(s) 294
MvnI CGCG 2 cut(s) 106, 108
MwoI GCNNNNNNNGC 1 cut(s) 469
NarI GGCGCC 1 cut(s) 432
NciI CCSGG 1 cut(s) 294
NcoI CCATGG 1 cut(s) 278
NdeII GATC 3 cut(s) 305, 315, 340
NlaIII CATG 2 cut(s) 44, 282
NlaIV GGNNCC 2 cut(s) 433, 675
NmeAIII GCCGAG 1 cut(s) 349
NmuCI GTSAC 1 cut(s) 370
PaeR7I CTCGAG 3 cut(s) 301, 481, 718
PcsI WCGNNNNNNNCGW 3 cut(s) 456, 554, 716
PdmI GAANNNNTTC 1 cut(s) 18
PfeI GAWTC 2 cut(s) 328, 550
Pfl23II CGTACG 1 cut(s) 519
PflFI GACNNNGTC 1 cut(s) 709
PfoI TCCNGGA 1 cut(s) 292
PkrI GCNGC 4 cut(s) 105, 285, 431, 764
PleI GAGTC 1 cut(s) 130
PluTI GGCGCC 1 cut(s) 435
PmaCI CACGTG 1 cut(s) 154
PmlI CACGTG 1 cut(s) 154
PpsI GAGTC 1 cut(s) 130
Ppu21I YACGTR 2 cut(s) 154, 580
PshBI ATTAAT 1 cut(s) 651
PsiI TTATAA 1 cut(s) 53
PspCI CACGTG 1 cut(s) 154
PspEI GGTNACC 1 cut(s) 590
PspLI CGTACG 1 cut(s) 519
PspN4I GGNNCC 2 cut(s) 433, 675
PspPI GGNCC 2 cut(s) 381, 486
PspXI VCTCGAGB 1 cut(s) 481
PsyI GACNNNGTC 1 cut(s) 709
RsaI GTAC 2 cut(s) 521, 578
RsaNI GTAC 2 cut(s) 520, 577
RseI CAYNNNNRTG 1 cut(s) 413
SaqAI TTAA 1 cut(s) 651
SatI GCNGC 4 cut(s) 104, 284, 430, 763
Sau3AI GATC 3 cut(s) 305, 315, 340
Sau96I GGNCC 2 cut(s) 381, 486
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 1 cut(s) 294
SduI GDGCHC 1 cut(s) 465
SfoI GGCGCC 1 cut(s) 433
Sfr274I CTCGAG 3 cut(s) 301, 481, 718
SinI GGWCC 1 cut(s) 486
SlaI CTCGAG 3 cut(s) 301, 481, 718
SmiMI CAYNNNNRTG 1 cut(s) 413
SmlI CTYRAG 3 cut(s) 301, 481, 718
SmoI CTYRAG 3 cut(s) 301, 481, 718
Sse9I AATT 9 cut(s) 4, 48, 83, 127, 134, 267, 288, 335, 751
SsiI CCGC 5 cut(s) 104, 220, 321, 429, 762
SspDI GGCGCC 1 cut(s) 431
SspI AATATT 1 cut(s) 655
SspMI CTAG 1 cut(s) 257
StyD4I CCNGG 1 cut(s) 292
StyI CCWWGG 1 cut(s) 278
TaaI ACNGT 5 cut(s) 313, 376, 395, 496, 535
TaiI ACGT 5 cut(s) 48, 156, 518, 582, 710
TaqI TCGA 7 cut(s) 302, 308, 343, 442, 465, 482, 719
TasI AATT 9 cut(s) 4, 48, 83, 127, 134, 267, 288, 335, 751
TauI GCSGC 3 cut(s) 106, 432, 765
TfiI GAWTC 2 cut(s) 328, 550
Tru1I TTAA 1 cut(s) 651
Tru9I TTAA 1 cut(s) 651
TseFI GTSAC 1 cut(s) 370
TseI GCWGC 1 cut(s) 283
Tsp45I GTSAC 1 cut(s) 370
TspDTI ATGAA 3 cut(s) 17, 260, 683
TspGWI ACGGA 3 cut(s) 187, 425, 543
Tth111I GACNNNGTC 1 cut(s) 709
VpaK11BI GGWCC 1 cut(s) 486
VspI ATTAAT 1 cut(s) 651
XhoI CTCGAG 3 cut(s) 301, 481, 718
XmnI GAANNNNTTC 1 cut(s) 18
XspI CTAG 1 cut(s) 257
ZraI GACGTC 2 cut(s) 516, 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.