RchiOBHm_Chr1g0352721

BON1-associated protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002449
Physical Location & Seq
Reverse (-)
46164539 .. 46165286
748 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57840

Sequence Viewer

Length: 549 bp
ATGGCAACTCGATATATAGAACTGACCGTTATCGCAGCCGAAAACCTCCAATCCAATCGCAAACCCTTGAAGAAAAACTCCTTCGTCACTGTCCGGACTGACGCCACCGTCACCACCTCCAAATCGTACCGGACGGCGGAGACGGACTCCGAAGGCGGAGGGCCAAGATGGAATGAGAAGTTAGTGATCGAGGTTCCTACACAAGCCAGGAATCTGATCATGGAAGTGCAATGCCAGAACGGGGCGAGGACGATCGGAGCGGCGACTATTCCGTTGACGGACTTCATCGGCGGTTGGGTGCCCGAGAGCTACATGCATTTTCTGAGTTACAGGCTGAGGGATGAGAGAGGTGTCAGGAATGGGATTATGAATATCTCGGTGAGGATGAAGGTGGACGAAGGTGCGACCTCGACGTACTCGAGGAAGTTGTCGAGTGGTTGCGCTAAGACGTCGTCGCAGACCAGGTTGGGGTATCCGGCGGTGGATAACAGTGGCGTCGTAACTGGGGTTCCGGTGAATTGGAATAGCTATTCTCGAGTTCAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

182

Amino Acids

20.16

Weight (kDa)

9.57

Isoelectric Point (pI)

35.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 5 - 95 5.1e-08 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 107
AatII GACGTC 1 cut(s) 452
AccB1I GGYRCC 1 cut(s) 298
AccBSI CCGCTC 1 cut(s) 260
AccIII TCCGGA 1 cut(s) 93
AciI CCGC 5 cut(s) 137, 156, 260, 291, 479
AcyI GRCGYC 3 cut(s) 102, 449, 495
AfaI GTAC 2 cut(s) 128, 416
AfiI CCNNNNNNNGG 3 cut(s) 136, 241, 468
AgsI TTSAA 2 cut(s) 70, 542
AjnI CCWGG 2 cut(s) 206, 461
AluBI AGCT 2 cut(s) 309, 528
AluI AGCT 2 cut(s) 309, 528
Alw26I GTCTC 1 cut(s) 134
Ama87I CYCGRG 3 cut(s) 302, 418, 534
Aor13HI TCCGGA 1 cut(s) 93
AoxI GGCC 1 cut(s) 161
ApeKI GCWGC 1 cut(s) 35
AspLEI GCGC 1 cut(s) 443
AspS9I GGNCC 1 cut(s) 161
AsuHPI GGTGA 3 cut(s) 103, 391, 526
AvaI CYCGRG 3 cut(s) 302, 418, 534
BaeGI GKGCMC 1 cut(s) 303
BanI GGYRCC 1 cut(s) 298
BbvCI CCTCAGC 1 cut(s) 335
BbvI GCAGC 1 cut(s) 47
BccI CCATC 1 cut(s) 162
BceAI ACGGC 1 cut(s) 150
BciT130I CCWGG 2 cut(s) 208, 463
BciVI GTATCC 1 cut(s) 483
BclI TGATCA 1 cut(s) 216
BcoDI GTCTC 1 cut(s) 134
BfuI GTATCC 1 cut(s) 483
BisI GCNGC 2 cut(s) 36, 261
BlsI GCNGC 2 cut(s) 37, 262
Bme1390I CCNGG 2 cut(s) 208, 463
BmeT110I CYCGRG 3 cut(s) 302, 418, 534
BmgT120I GGNCC 1 cut(s) 161
BmiI GGNNCC 3 cut(s) 195, 300, 510
BmrFI CCNGG 2 cut(s) 208, 463
BmrI ACTGGG 1 cut(s) 513
BmuI ACTGGG 1 cut(s) 513
BplI GAGNNNNNCTC 2 cut(s) 131, 163
Bpu10I CCTNAGC 1 cut(s) 335
BsaHI GRCGYC 3 cut(s) 102, 449, 495
BsaWI WCCGGW 3 cut(s) 93, 129, 511
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 241, 468
Bse1I ACTGG 1 cut(s) 508
Bse3DI GCAATG 1 cut(s) 236
BseAI TCCGGA 1 cut(s) 93
BseBI CCWGG 2 cut(s) 208, 463
BseGI GGATG 2 cut(s) 346, 390
BseLI CCNNNNNNNGG 3 cut(s) 136, 241, 468
BseMI GCAATG 1 cut(s) 236
BseMII CTCAG 2 cut(s) 314, 326
BseNI ACTGG 1 cut(s) 508
BseSI GKGCMC 1 cut(s) 303
BseXI GCAGC 1 cut(s) 47
Bsh1285I CGRYCG 1 cut(s) 255
BshFI GGCC 1 cut(s) 163
BshNI GGYRCC 1 cut(s) 298
BsiEI CGRYCG 1 cut(s) 255
BsiHKCI CYCGRG 3 cut(s) 302, 418, 534
BsiSI CCGG 4 cut(s) 94, 130, 476, 512
BslI CCNNNNNNNGG 3 cut(s) 136, 241, 468
BsmAI GTCTC 1 cut(s) 134
BsmBI CGTCTC 1 cut(s) 134
BsnI GGCC 1 cut(s) 163
BsoBI CYCGRG 3 cut(s) 302, 418, 534
Bsp1286I GDGCHC 1 cut(s) 303
Bsp13I TCCGGA 1 cut(s) 93
Bsp143I GATC 3 cut(s) 186, 216, 252
BspACI CCGC 5 cut(s) 137, 156, 260, 291, 479
BspANI GGCC 1 cut(s) 163
BspCNI CTCAG 2 cut(s) 315, 327
BspEI TCCGGA 1 cut(s) 93
BspLI GGNNCC 3 cut(s) 195, 300, 510
BspT107I GGYRCC 1 cut(s) 298
BsrBI CCGCTC 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 236
BsrI ACTGG 1 cut(s) 508
BssMI GATC 3 cut(s) 186, 216, 252
BssNI GRCGYC 3 cut(s) 102, 449, 495
Bst2UI CCWGG 2 cut(s) 208, 463
Bst4CI ACNGT 4 cut(s) 28, 91, 109, 491
BstACI GRCGYC 3 cut(s) 102, 449, 495
BstDEI CTNAG 3 cut(s) 323, 335, 444
BstF5I GGATG 2 cut(s) 346, 390
BstHHI GCGC 1 cut(s) 443
BstKTI GATC 3 cut(s) 189, 219, 255
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 3 cut(s) 186, 216, 252
BstMCI CGRYCG 1 cut(s) 255
BstNI CCWGG 2 cut(s) 208, 463
BstNSI RCATGY 1 cut(s) 316
BstSCI CCNGG 2 cut(s) 206, 461
BstSLI GKGCMC 1 cut(s) 303
BstV1I GCAGC 1 cut(s) 47
BsuI GTATCC 1 cut(s) 483
BsuRI GGCC 1 cut(s) 163
BtsCI GGATG 2 cut(s) 346, 390
BtsIMutI CAGTG 2 cut(s) 87, 496
CfoI GCGC 1 cut(s) 443
Cfr13I GGNCC 1 cut(s) 161
CseI GACGC 2 cut(s) 110, 484
CsiI ACCWGGT 1 cut(s) 461
Csp6I GTAC 2 cut(s) 127, 415
CviAII CATG 2 cut(s) 220, 313
CviJI RGCY 6 cut(s) 38, 163, 206, 309, 334, 528
CviKI_1 RGCY 6 cut(s) 38, 163, 206, 309, 334, 528
CviQI GTAC 2 cut(s) 127, 415
DdeI CTNAG 3 cut(s) 323, 335, 444
DpnI GATC 3 cut(s) 188, 218, 254
DpnII GATC 3 cut(s) 186, 216, 252
DrdI GACNNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 107
EciI GGCGGA 2 cut(s) 152, 171
Eco88I CYCGRG 3 cut(s) 302, 418, 534
EcoRII CCWGG 2 cut(s) 206, 461
EcoT22I ATGCAT 1 cut(s) 318
Esp3I CGTCTC 1 cut(s) 134
FaeI CATG 2 cut(s) 223, 316
FaiI YATR 5 cut(s) 15, 17, 221, 314, 368
FatI CATG 2 cut(s) 219, 312
FbaI TGATCA 1 cut(s) 216
Fnu4HI GCNGC 2 cut(s) 36, 261
FokI GGATG 2 cut(s) 353, 397
Fsp4HI GCNGC 2 cut(s) 36, 261
GlaI GCGC 1 cut(s) 442
GluI GCNGC 2 cut(s) 36, 261
HaeIII GGCC 1 cut(s) 163
HapII CCGG 4 cut(s) 94, 130, 476, 512
HgaI GACGC 2 cut(s) 110, 484
HhaI GCGC 1 cut(s) 443
Hin1I GRCGYC 3 cut(s) 102, 449, 495
Hin1II CATG 2 cut(s) 223, 316
Hin6I GCGC 1 cut(s) 441
HinP1I GCGC 1 cut(s) 441
HincII GTYRAC 1 cut(s) 276
HindII GTYRAC 1 cut(s) 276
HinfI GANTC 2 cut(s) 146, 211
HpaII CCGG 4 cut(s) 94, 130, 476, 512
HphI GGTGA 3 cut(s) 103, 391, 526
Hpy166II GTNNAC 2 cut(s) 276, 394
Hpy188I TCNGA 4 cut(s) 151, 216, 257, 324
Hpy188III TCNNGA 3 cut(s) 94, 355, 534
Hpy8I GTNNAC 2 cut(s) 276, 394
Hpy99I CGWCG 4 cut(s) 415, 454, 457, 500
HpyAV CCTTC 4 cut(s) 91, 146, 382, 392
HpyCH4III ACNGT 4 cut(s) 28, 91, 109, 491
HpyCH4IV ACGT 2 cut(s) 413, 449
HpyCH4V TGCA 2 cut(s) 229, 316
HpyF3I CTNAG 3 cut(s) 323, 335, 444
HpySE526I ACGT 2 cut(s) 413, 449
Hsp92I GRCGYC 3 cut(s) 102, 449, 495
Hsp92II CATG 2 cut(s) 223, 316
HspAI GCGC 1 cut(s) 441
Kpn2I TCCGGA 1 cut(s) 93
Ksp22I TGATCA 1 cut(s) 216
Kzo9I GATC 3 cut(s) 186, 216, 252
LmnI GCTCC 1 cut(s) 257
Lsp1109I GCAGC 1 cut(s) 47
MabI ACCWGGT 1 cut(s) 461
MaeII ACGT 2 cut(s) 413, 449
MaeIII GTNAC 4 cut(s) 85, 109, 326, 499
MalI GATC 3 cut(s) 188, 218, 254
MbiI CCGCTC 1 cut(s) 260
MboI GATC 3 cut(s) 186, 216, 252
MboII GAAGA 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 303
MluCI AATT 1 cut(s) 517
MlyI GAGTC 1 cut(s) 140
Mph1103I ATGCAT 1 cut(s) 318
MroI TCCGGA 1 cut(s) 93
MslI CAYNNNNRTG 1 cut(s) 224
MspI CCGG 4 cut(s) 94, 130, 476, 512
MspR9I CCNGG 2 cut(s) 208, 463
MvaI CCWGG 2 cut(s) 208, 463
NdeII GATC 3 cut(s) 186, 216, 252
NlaIII CATG 2 cut(s) 223, 316
NlaIV GGNNCC 3 cut(s) 195, 300, 510
NmuCI GTSAC 2 cut(s) 85, 109
NsiI ATGCAT 1 cut(s) 318
NspI RCATGY 1 cut(s) 316
PaeR7I CTCGAG 2 cut(s) 418, 534
PcsI WCGNNNNNNNCGW 1 cut(s) 416
PfeI GAWTC 1 cut(s) 211
PflFI GACNNNGTC 1 cut(s) 451
PkrI GCNGC 2 cut(s) 37, 262
Ple19I CGATCG 1 cut(s) 255
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
Psp6I CCWGG 2 cut(s) 206, 461
PspGI CCWGG 2 cut(s) 206, 461
PspN4I GGNNCC 3 cut(s) 195, 300, 510
PspPI GGNCC 1 cut(s) 161
PspXI VCTCGAGB 1 cut(s) 418
PsrI GAACNNNNNNTAC 2 cut(s) 492, 524
PsyI GACNNNGTC 1 cut(s) 451
PvuI CGATCG 1 cut(s) 255
RsaI GTAC 2 cut(s) 128, 416
RsaNI GTAC 2 cut(s) 127, 415
RseI CAYNNNNRTG 1 cut(s) 224
SatI GCNGC 2 cut(s) 36, 261
Sau3AI GATC 3 cut(s) 186, 216, 252
Sau96I GGNCC 1 cut(s) 161
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 2 cut(s) 208, 463
SduI GDGCHC 1 cut(s) 303
SexAI ACCWGGT 1 cut(s) 461
Sfr274I CTCGAG 2 cut(s) 418, 534
SlaI CTCGAG 2 cut(s) 418, 534
SmiMI CAYNNNNRTG 1 cut(s) 224
SmlI CTYRAG 2 cut(s) 418, 534
SmoI CTYRAG 2 cut(s) 418, 534
Sse9I AATT 1 cut(s) 517
SsiI CCGC 5 cut(s) 137, 156, 260, 291, 479
SspI AATATT 1 cut(s) 545
StyD4I CCNGG 2 cut(s) 206, 461
TaaI ACNGT 4 cut(s) 28, 91, 109, 491
TaiI ACGT 2 cut(s) 416, 452
TaqI TCGA 6 cut(s) 10, 189, 410, 419, 431, 535
TasI AATT 1 cut(s) 517
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 1 cut(s) 211
TscAI CASTG 2 cut(s) 94, 496
TseFI GTSAC 2 cut(s) 85, 109
TseI GCWGC 1 cut(s) 35
Tsp45I GTSAC 2 cut(s) 85, 109
TspDTI ATGAA 3 cut(s) 274, 383, 401
TspGWI ACGGA 3 cut(s) 158, 261, 293
TspRI CASTG 2 cut(s) 94, 496
Tth111I GACNNNGTC 1 cut(s) 451
XceI RCATGY 1 cut(s) 316
XhoI CTCGAG 2 cut(s) 418, 534
ZraI GACGTC 1 cut(s) 450
Zsp2I ATGCAT 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.