Rroxscaffold_4G00302390

BON1-associated protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002449
Physical Location & Seq
Forward (+)
22791816 .. 22792352
537 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00302390.1

Sequence Viewer

Length: 537 bp
ATGACGTCTCGATCTGTAGAAATCACTGTTCTCTCCGCAGAAAATCTGCAATCAAACCGAAAACCCTTCAAAAAAAACTCCTTCGTTACTGTGCGGACCGACGCTGCCGTATCCACCTCCAAGTTGTACCGGACGGCGGAGACTGACTGCGAAGGCGGCGGTCCAAGGTGGAACGAGAAGATTGAGGTCGAACTTCCGGCGCAGGCTAGGAATCTGATATTGGAAGTACAATGCAAGAACGGTGCAAGAACGATCGGAGCGGCGAGCATTCCGGTGTCGGACTTCACCTGCGGGTGGGTGCCTGAGAACTACTTGCATTTTCTGAGTTATAGGTTGAGGGATGAGAGAGGTGTGAGGAATGGGATTATTAATATCTCGGTGAGCATGAAGGTTCCGGAAGGTTTGATGTCGACGTACGCCAGGAAGGTGACGAGTGGGTGTGGTACGACGTCGTCGCAGACCAAGGTGGGGTATCCGGCGGTGGAGAACAGTGGTGTTGCAACTGGTGTTCCGCTGAATTGGAATAGCTATCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

178

Amino Acids

19.45

Weight (kDa)

9.12

Isoelectric Point (pI)

36.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 5 - 95 8.4e-08 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 296
AatII GACGTC 2 cut(s) 8, 452
Acc36I ACCTGC 1 cut(s) 296
AccB1I GGYRCC 1 cut(s) 298
AccBSI CCGCTC 1 cut(s) 260
AccI GTMKAC 1 cut(s) 410
AccIII TCCGGA 1 cut(s) 394
AciI CCGC 9 cut(s) 36, 94, 137, 156, 159, 260, 291, 479, 512
AcyI GRCGYC 2 cut(s) 5, 449
AfaI GTAC 4 cut(s) 128, 228, 416, 445
AfiI CCNNNNNNNGG 3 cut(s) 136, 294, 468
AgsI TTSAA 1 cut(s) 70
AjnI CCWGG 1 cut(s) 419
AjuI GAANNNNNNNTTGG 2 cut(s) 203, 235
AluBI AGCT 1 cut(s) 528
AluI AGCT 1 cut(s) 528
Alw26I GTCTC 2 cut(s) 12, 134
Aor13HI TCCGGA 1 cut(s) 394
ApeKI GCWGC 1 cut(s) 104
AseI ATTAAT 1 cut(s) 369
AspLEI GCGC 1 cut(s) 202
AspS9I GGNCC 2 cut(s) 96, 161
AsuHPI GGTGA 3 cut(s) 277, 391, 439
AvaII GGWCC 2 cut(s) 96, 161
BanI GGYRCC 1 cut(s) 298
BbvI GCAGC 1 cut(s) 91
BceAI ACGGC 2 cut(s) 92, 150
BciT130I CCWGG 1 cut(s) 421
BciVI GTATCC 2 cut(s) 121, 483
BcoDI GTCTC 2 cut(s) 12, 134
BfaI CTAG 1 cut(s) 207
BfmI CTRYAG 1 cut(s) 15
BfuAI ACCTGC 1 cut(s) 296
BfuI GTATCC 2 cut(s) 121, 483
BisI GCNGC 3 cut(s) 105, 157, 261
BlsI GCNGC 3 cut(s) 106, 158, 262
Bme1390I CCNGG 1 cut(s) 421
Bme18I GGWCC 2 cut(s) 96, 161
BmgT120I GGNCC 2 cut(s) 96, 161
BmiI GGNNCC 2 cut(s) 300, 393
BmrFI CCNGG 1 cut(s) 421
BsaHI GRCGYC 2 cut(s) 5, 449
BsaJI CCNNGG 2 cut(s) 164, 462
BsaWI WCCGGW 3 cut(s) 129, 271, 394
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 294, 468
Bse1I ACTGG 1 cut(s) 508
BseAI TCCGGA 1 cut(s) 394
BseBI CCWGG 1 cut(s) 421
BseDI CCNNGG 2 cut(s) 164, 462
BseGI GGATG 1 cut(s) 346
BseLI CCNNNNNNNGG 3 cut(s) 136, 294, 468
BseMII CTCAG 2 cut(s) 294, 314
BseNI ACTGG 1 cut(s) 508
BseXI GCAGC 1 cut(s) 91
Bsh1285I CGRYCG 1 cut(s) 255
BshNI GGYRCC 1 cut(s) 298
BsiEI CGRYCG 1 cut(s) 255
BsiSI CCGG 5 cut(s) 130, 197, 272, 395, 476
BsiWI CGTACG 1 cut(s) 414
BslI CCNNNNNNNGG 3 cut(s) 136, 294, 468
BsmAI GTCTC 2 cut(s) 12, 134
BsmBI CGTCTC 1 cut(s) 12
BsmI GAATGC 1 cut(s) 267
Bsp13I TCCGGA 1 cut(s) 394
Bsp143I GATC 2 cut(s) 11, 252
BspACI CCGC 9 cut(s) 36, 94, 137, 156, 159, 260, 291, 479, 512
BspCNI CTCAG 2 cut(s) 295, 315
BspEI TCCGGA 1 cut(s) 394
BspLI GGNNCC 2 cut(s) 300, 393
BspMI ACCTGC 1 cut(s) 296
BspT107I GGYRCC 1 cut(s) 298
BsrBI CCGCTC 1 cut(s) 260
BsrI ACTGG 1 cut(s) 508
BssECI CCNNGG 2 cut(s) 164, 462
BssMI GATC 2 cut(s) 11, 252
BssNI GRCGYC 2 cut(s) 5, 449
BssT1I CCWWGG 2 cut(s) 164, 462
Bst2UI CCWGG 1 cut(s) 421
Bst4CI ACNGT 4 cut(s) 28, 91, 242, 491
BstACI GRCGYC 2 cut(s) 5, 449
BstC8I GCNNGC 2 cut(s) 204, 265
BstDEI CTNAG 2 cut(s) 303, 323
BstF5I GGATG 1 cut(s) 346
BstHHI GCGC 1 cut(s) 202
BstKTI GATC 2 cut(s) 14, 255
BstMAI GTCTC 2 cut(s) 12, 134
BstMBI GATC 2 cut(s) 11, 252
BstMCI CGRYCG 1 cut(s) 255
BstMWI GCNNNNNNNGC 1 cut(s) 156
BstNI CCWGG 1 cut(s) 421
BstSCI CCNGG 1 cut(s) 419
BstSFI CTRYAG 1 cut(s) 15
BstV1I GCAGC 1 cut(s) 91
BsuI GTATCC 2 cut(s) 121, 483
BtsCI GGATG 1 cut(s) 346
BtsIMutI CAGTG 2 cut(s) 24, 496
BveI ACCTGC 1 cut(s) 296
Cac8I GCNNGC 2 cut(s) 204, 265
CfoI GCGC 1 cut(s) 202
Cfr13I GGNCC 2 cut(s) 96, 161
CpoI CGGWCCG 1 cut(s) 96
CseI GACGC 1 cut(s) 110
Csp6I GTAC 4 cut(s) 127, 227, 415, 444
CspI CGGWCCG 1 cut(s) 96
CviAII CATG 1 cut(s) 385
CviJI RGCY 2 cut(s) 206, 528
CviKI_1 RGCY 2 cut(s) 206, 528
CviQI GTAC 4 cut(s) 127, 227, 415, 444
DdeI CTNAG 2 cut(s) 303, 323
DpnI GATC 2 cut(s) 13, 254
DpnII GATC 2 cut(s) 11, 252
EciI GGCGGA 1 cut(s) 152
Eco130I CCWWGG 2 cut(s) 164, 462
Eco47I GGWCC 2 cut(s) 96, 161
EcoRII CCWGG 1 cut(s) 419
EcoT14I CCWWGG 2 cut(s) 164, 462
ErhI CCWWGG 2 cut(s) 164, 462
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 1 cut(s) 388
FaiI YATR 2 cut(s) 330, 386
FatI CATG 1 cut(s) 384
FauI CCCGC 1 cut(s) 284
FblI GTMKAC 1 cut(s) 410
Fnu4HI GCNGC 3 cut(s) 105, 157, 261
FokI GGATG 1 cut(s) 353
Fsp4HI GCNGC 3 cut(s) 105, 157, 261
FspBI CTAG 1 cut(s) 207
GlaI GCGC 1 cut(s) 201
GluI GCNGC 3 cut(s) 105, 157, 261
HapII CCGG 5 cut(s) 130, 197, 272, 395, 476
HgaI GACGC 1 cut(s) 110
HhaI GCGC 1 cut(s) 202
Hin1I GRCGYC 2 cut(s) 5, 449
Hin1II CATG 1 cut(s) 388
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HincII GTYRAC 1 cut(s) 411
HindII GTYRAC 1 cut(s) 411
HinfI GANTC 1 cut(s) 211
HpaII CCGG 5 cut(s) 130, 197, 272, 395, 476
HphI GGTGA 3 cut(s) 277, 391, 439
Hpy166II GTNNAC 1 cut(s) 411
Hpy188I TCNGA 4 cut(s) 216, 257, 280, 324
Hpy188III TCNNGA 2 cut(s) 9, 395
Hpy8I GTNNAC 1 cut(s) 411
Hpy99I CGWCG 5 cut(s) 104, 415, 451, 454, 457
HpyAV CCTTC 6 cut(s) 76, 91, 146, 382, 392, 418
HpyCH4III ACNGT 4 cut(s) 28, 91, 242, 491
HpyCH4IV ACGT 3 cut(s) 5, 413, 449
HpyCH4V TGCA 5 cut(s) 49, 234, 245, 316, 500
HpyF10VI GCNNNNNNNGC 1 cut(s) 156
HpyF3I CTNAG 2 cut(s) 303, 323
HpySE526I ACGT 3 cut(s) 5, 413, 449
Hsp92I GRCGYC 2 cut(s) 5, 449
Hsp92II CATG 1 cut(s) 388
HspAI GCGC 1 cut(s) 200
Kpn2I TCCGGA 1 cut(s) 394
Kzo9I GATC 2 cut(s) 11, 252
LmnI GCTCC 1 cut(s) 257
Lsp1109I GCAGC 1 cut(s) 91
MaeI CTAG 1 cut(s) 207
MaeII ACGT 3 cut(s) 5, 413, 449
MaeIII GTNAC 2 cut(s) 85, 427
MalI GATC 2 cut(s) 13, 254
MbiI CCGCTC 1 cut(s) 260
MboI GATC 2 cut(s) 11, 252
MboII GAAGA 1 cut(s) 190
MluCI AATT 1 cut(s) 517
MmeI TCCRAC 1 cut(s) 258
MnlI CCTC 5 cut(s) 127, 178, 330, 341, 348
MroI TCCGGA 1 cut(s) 394
MseI TTAA 1 cut(s) 369
MslI CAYNNNNRTG 1 cut(s) 272
MspA1I CMGCKG 1 cut(s) 514
MspI CCGG 5 cut(s) 130, 197, 272, 395, 476
MspR9I CCNGG 1 cut(s) 421
Mva1269I GAATGC 1 cut(s) 267
MvaI CCWGG 1 cut(s) 421
MwoI GCNNNNNNNGC 1 cut(s) 156
NdeII GATC 2 cut(s) 11, 252
NlaIII CATG 1 cut(s) 388
NlaIV GGNNCC 2 cut(s) 300, 393
NmuCI GTSAC 1 cut(s) 427
PaqCI CACCTGC 1 cut(s) 296
PctI GAATGC 1 cut(s) 267
PfeI GAWTC 1 cut(s) 211
Pfl23II CGTACG 1 cut(s) 414
PflFI GACNNNGTC 1 cut(s) 451
PkrI GCNGC 3 cut(s) 106, 158, 262
Ple19I CGATCG 1 cut(s) 255
PshBI ATTAAT 1 cut(s) 369
Psp6I CCWGG 1 cut(s) 419
PspGI CCWGG 1 cut(s) 419
PspLI CGTACG 1 cut(s) 414
PspN4I GGNNCC 2 cut(s) 300, 393
PspPI GGNCC 2 cut(s) 96, 161
PsyI GACNNNGTC 1 cut(s) 451
PvuI CGATCG 1 cut(s) 255
RsaI GTAC 4 cut(s) 128, 228, 416, 445
RsaNI GTAC 4 cut(s) 127, 227, 415, 444
RseI CAYNNNNRTG 1 cut(s) 272
Rsr2I CGGWCCG 1 cut(s) 96
RsrII CGGWCCG 1 cut(s) 96
SalI GTCGAC 1 cut(s) 409
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 3 cut(s) 105, 157, 261
Sau3AI GATC 2 cut(s) 11, 252
Sau96I GGNCC 2 cut(s) 96, 161
ScrFI CCNGG 1 cut(s) 421
SfcI CTRYAG 1 cut(s) 15
SinI GGWCC 2 cut(s) 96, 161
SmiMI CAYNNNNRTG 1 cut(s) 272
Sse9I AATT 1 cut(s) 517
SsiI CCGC 9 cut(s) 36, 94, 137, 156, 159, 260, 291, 479, 512
SspMI CTAG 1 cut(s) 207
StyD4I CCNGG 1 cut(s) 419
StyI CCWWGG 2 cut(s) 164, 462
TaaI ACNGT 4 cut(s) 28, 91, 242, 491
TaiI ACGT 3 cut(s) 8, 416, 452
TaqI TCGA 3 cut(s) 10, 189, 410
TaqII GACCGA 1 cut(s) 113
TasI AATT 1 cut(s) 517
TatI WGTACW 1 cut(s) 226
TauI GCSGC 2 cut(s) 159, 263
TfiI GAWTC 1 cut(s) 211
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 2 cut(s) 31, 496
TseFI GTSAC 1 cut(s) 427
TseI GCWGC 1 cut(s) 104
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 1 cut(s) 401
TspRI CASTG 2 cut(s) 31, 496
Tth111I GACNNNGTC 1 cut(s) 451
VpaK11BI GGWCC 2 cut(s) 96, 161
VspI ATTAAT 1 cut(s) 369
XmiI GTMKAC 1 cut(s) 410
XspI CTAG 1 cut(s) 207
ZraI GACGTC 2 cut(s) 6, 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.