Rh1DG241300

BON1-associated protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0002449
Physical Location & Seq
Reverse (-)
45706006 .. 45708540
2535 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG241300.1

Sequence Viewer

Length: 474 bp
ATGGCAACTCGATATATAGAACTGACCGTTATCGCAGCCGAAAACCTCCAATCCAATCGCAAACCCTTGAAGAAAAACTCCTTCGTCACCGTCCGGACCGACGCCACCGTCACCGCCTCCAAATCGTACCGGACGGTGGAGACGGACTCCAAAGGCGGAGGGCCAAGATGGAATGAGAAGTTAGTGATCGAGGTTCCTGCGCAAGCCAGGAATCTGATCATGGAAGTGCAATGCCAGAACGGGGCGAGGACGATTGGAGCGGCGACTATTCCGTTGACGGACTTCATCGGCGGTTGGGTGGACGAAGGTGCGACGTCGATGTATGCGAGGAAGTTGTCGAGTGGTTGCGCTACGACGTCGTCGCAGACTAGGTTGGGCTATCCGGCAGTGGATAACAGTGGCGTTGTAACTGGGGTTCCGTGTAGAGTTGGGTTGCGGTTCATTCAACATTACAACGAGCTTACATTACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

157

Amino Acids

17.06

Weight (kDa)

9.3

Isoelectric Point (pI)

32.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 5 - 95 1.6e-08 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 107
AatII GACGTC 2 cut(s) 317, 359
Acc16I TGCGCA 1 cut(s) 201
AccBSI CCGCTC 1 cut(s) 260
AccIII TCCGGA 1 cut(s) 93
AciI CCGC 5 cut(s) 114, 156, 260, 291, 436
AcyI GRCGYC 3 cut(s) 102, 314, 356
AfaI GTAC 1 cut(s) 128
AfiI CCNNNNNNNGG 2 cut(s) 136, 241
AgsI TTSAA 2 cut(s) 70, 446
AjnI CCWGG 1 cut(s) 206
AluBI AGCT 1 cut(s) 460
AluI AGCT 1 cut(s) 460
Alw26I GTCTC 1 cut(s) 134
Aor13HI TCCGGA 1 cut(s) 93
AoxI GGCC 1 cut(s) 161
ApeKI GCWGC 1 cut(s) 35
AspLEI GCGC 2 cut(s) 202, 350
AspS9I GGNCC 2 cut(s) 96, 161
AsuHPI GGTGA 2 cut(s) 79, 103
AvaII GGWCC 1 cut(s) 96
BbvI GCAGC 1 cut(s) 47
BccI CCATC 1 cut(s) 162
BcgI CGANNNNNNTGC 2 cut(s) 179, 213
BciT130I CCWGG 1 cut(s) 208
BclI TGATCA 1 cut(s) 216
BcoDI GTCTC 1 cut(s) 134
BfaI CTAG 1 cut(s) 369
BisI GCNGC 2 cut(s) 36, 261
BlsI GCNGC 2 cut(s) 37, 262
Bme1390I CCNGG 1 cut(s) 208
Bme18I GGWCC 1 cut(s) 96
BmgT120I GGNCC 2 cut(s) 96, 161
BmiI GGNNCC 2 cut(s) 195, 417
BmrFI CCNGG 1 cut(s) 208
BmrI ACTGGG 1 cut(s) 420
BmuI ACTGGG 1 cut(s) 420
BplI GAGNNNNNCTC 2 cut(s) 131, 163
BsaHI GRCGYC 3 cut(s) 102, 314, 356
BsaWI WCCGGW 2 cut(s) 93, 129
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 241
Bse1I ACTGG 1 cut(s) 415
Bse3DI GCAATG 1 cut(s) 236
BseAI TCCGGA 1 cut(s) 93
BseBI CCWGG 1 cut(s) 208
BseLI CCNNNNNNNGG 2 cut(s) 136, 241
BseMI GCAATG 1 cut(s) 236
BseNI ACTGG 1 cut(s) 415
BseXI GCAGC 1 cut(s) 47
BshFI GGCC 1 cut(s) 163
BsiSI CCGG 3 cut(s) 94, 130, 383
BslI CCNNNNNNNGG 2 cut(s) 136, 241
BsmAI GTCTC 1 cut(s) 134
BsmBI CGTCTC 1 cut(s) 134
BsnI GGCC 1 cut(s) 163
Bsp13I TCCGGA 1 cut(s) 93
Bsp143I GATC 2 cut(s) 186, 216
BspACI CCGC 5 cut(s) 114, 156, 260, 291, 436
BspANI GGCC 1 cut(s) 163
BspEI TCCGGA 1 cut(s) 93
BspLI GGNNCC 2 cut(s) 195, 417
BsrBI CCGCTC 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 236
BsrI ACTGG 1 cut(s) 415
BssMI GATC 2 cut(s) 186, 216
BssNI GRCGYC 3 cut(s) 102, 314, 356
Bst2UI CCWGG 1 cut(s) 208
Bst4CI ACNGT 5 cut(s) 28, 91, 109, 136, 398
BstACI GRCGYC 3 cut(s) 102, 314, 356
BstC8I GCNNGC 1 cut(s) 204
BstHHI GCGC 2 cut(s) 202, 350
BstKTI GATC 2 cut(s) 189, 219
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 2 cut(s) 186, 216
BstNI CCWGG 1 cut(s) 208
BstSCI CCNGG 1 cut(s) 206
BstV1I GCAGC 1 cut(s) 47
BsuRI GGCC 1 cut(s) 163
BtsI GCAGTG 1 cut(s) 393
BtsIMutI CAGTG 2 cut(s) 393, 403
Cac8I GCNNGC 1 cut(s) 204
CfoI GCGC 2 cut(s) 202, 350
Cfr13I GGNCC 2 cut(s) 96, 161
CpoI CGGWCCG 1 cut(s) 96
CseI GACGC 1 cut(s) 110
Csp6I GTAC 1 cut(s) 127
CspI CGGWCCG 1 cut(s) 96
CviAII CATG 1 cut(s) 220
CviJI RGCY 5 cut(s) 38, 163, 206, 378, 460
CviKI_1 RGCY 5 cut(s) 38, 163, 206, 378, 460
CviQI GTAC 1 cut(s) 127
DpnI GATC 2 cut(s) 188, 218
DpnII GATC 2 cut(s) 186, 216
DrdI GACNNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 107
EciI GGCGGA 1 cut(s) 171
Eco47I GGWCC 1 cut(s) 96
EcoRII CCWGG 1 cut(s) 206
Esp3I CGTCTC 1 cut(s) 134
FaeI CATG 1 cut(s) 223
FaiI YATR 4 cut(s) 15, 17, 221, 324
FatI CATG 1 cut(s) 219
FbaI TGATCA 1 cut(s) 216
Fnu4HI GCNGC 2 cut(s) 36, 261
Fsp4HI GCNGC 2 cut(s) 36, 261
FspBI CTAG 1 cut(s) 369
FspI TGCGCA 1 cut(s) 201
GlaI GCGC 2 cut(s) 201, 349
GluI GCNGC 2 cut(s) 36, 261
HaeIII GGCC 1 cut(s) 163
HapII CCGG 3 cut(s) 94, 130, 383
HgaI GACGC 1 cut(s) 110
HhaI GCGC 2 cut(s) 202, 350
Hin1I GRCGYC 3 cut(s) 102, 314, 356
Hin1II CATG 1 cut(s) 223
Hin6I GCGC 2 cut(s) 200, 348
HinP1I GCGC 2 cut(s) 200, 348
HincII GTYRAC 1 cut(s) 276
HindII GTYRAC 1 cut(s) 276
HinfI GANTC 2 cut(s) 146, 211
HpaII CCGG 3 cut(s) 94, 130, 383
HphI GGTGA 2 cut(s) 79, 103
Hpy166II GTNNAC 2 cut(s) 276, 301
Hpy188I TCNGA 1 cut(s) 216
Hpy188III TCNNGA 1 cut(s) 94
Hpy8I GTNNAC 2 cut(s) 276, 301
Hpy99I CGWCG 6 cut(s) 104, 316, 319, 358, 361, 364
HpyAV CCTTC 2 cut(s) 91, 299
HpyCH4III ACNGT 5 cut(s) 28, 91, 109, 136, 398
HpyCH4IV ACGT 2 cut(s) 314, 356
HpyCH4V TGCA 1 cut(s) 229
HpySE526I ACGT 2 cut(s) 314, 356
Hsp92I GRCGYC 3 cut(s) 102, 314, 356
Hsp92II CATG 1 cut(s) 223
HspAI GCGC 2 cut(s) 200, 348
Kpn2I TCCGGA 1 cut(s) 93
Ksp22I TGATCA 1 cut(s) 216
Kzo9I GATC 2 cut(s) 186, 216
LmnI GCTCC 1 cut(s) 257
LpnPI CCDG 8 cut(s) 107, 143, 193, 210, 220, 248, 396, 396
Lsp1109I GCAGC 1 cut(s) 47
MaeI CTAG 1 cut(s) 369
MaeII ACGT 2 cut(s) 314, 356
MaeIII GTNAC 3 cut(s) 85, 109, 406
MalI GATC 2 cut(s) 188, 218
MbiI CCGCTC 1 cut(s) 260
MboI GATC 2 cut(s) 186, 216
MboII GAAGA 1 cut(s) 82
MlyI GAGTC 1 cut(s) 140
MnlI CCTC 6 cut(s) 56, 127, 152, 184, 240, 321
MroI TCCGGA 1 cut(s) 93
MseI TTAA 1 cut(s) 472
MslI CAYNNNNRTG 1 cut(s) 224
MspI CCGG 3 cut(s) 94, 130, 383
MspR9I CCNGG 1 cut(s) 208
MvaI CCWGG 1 cut(s) 208
NdeII GATC 2 cut(s) 186, 216
NlaIII CATG 1 cut(s) 223
NlaIV GGNNCC 2 cut(s) 195, 417
NmuCI GTSAC 2 cut(s) 85, 109
NsbI TGCGCA 1 cut(s) 201
PcsI WCGNNNNNNNCGW 1 cut(s) 323
PfeI GAWTC 1 cut(s) 211
PflFI GACNNNGTC 1 cut(s) 358
PkrI GCNGC 2 cut(s) 37, 262
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
Psp6I CCWGG 1 cut(s) 206
PspGI CCWGG 1 cut(s) 206
PspN4I GGNNCC 2 cut(s) 195, 417
PspPI GGNCC 2 cut(s) 96, 161
PsrI GAACNNNNNNTAC 2 cut(s) 399, 431
PsyI GACNNNGTC 1 cut(s) 358
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
RseI CAYNNNNRTG 1 cut(s) 224
Rsr2I CGGWCCG 1 cut(s) 96
RsrII CGGWCCG 1 cut(s) 96
SaqAI TTAA 1 cut(s) 472
SatI GCNGC 2 cut(s) 36, 261
Sau3AI GATC 2 cut(s) 186, 216
Sau96I GGNCC 2 cut(s) 96, 161
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 1 cut(s) 208
SetI ASST 7 cut(s) 48, 195, 310, 317, 359, 374, 462
SinI GGWCC 1 cut(s) 96
SmiMI CAYNNNNRTG 1 cut(s) 224
SsiI CCGC 5 cut(s) 114, 156, 260, 291, 436
SspMI CTAG 1 cut(s) 369
StyD4I CCNGG 1 cut(s) 206
TaaI ACNGT 5 cut(s) 28, 91, 109, 136, 398
TaiI ACGT 2 cut(s) 317, 359
TaqI TCGA 4 cut(s) 10, 189, 317, 338
TaqII GACCGA 1 cut(s) 113
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 1 cut(s) 211
Tru1I TTAA 1 cut(s) 472
Tru9I TTAA 1 cut(s) 472
TscAI CASTG 2 cut(s) 393, 403
TseFI GTSAC 2 cut(s) 85, 109
TseI GCWGC 1 cut(s) 35
Tsp45I GTSAC 2 cut(s) 85, 109
TspDTI ATGAA 2 cut(s) 274, 430
TspGWI ACGGA 4 cut(s) 158, 261, 293, 408
TspRI CASTG 2 cut(s) 393, 403
Tth111I GACNNNGTC 1 cut(s) 358
VpaK11BI GGWCC 1 cut(s) 96
XspI CTAG 1 cut(s) 369
ZraI GACGTC 2 cut(s) 315, 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.