MD01G1049300.v1.1

BON1-associated protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002449
Physical Location & Seq
Forward (+)
15212100 .. 15212639
540 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1049300.v1.1.491

Sequence Viewer

Length: 540 bp
ATGGCAGCCAATTCCCGGACGCTCGAGATCACGGTCATCTCCGCCGAGAATCTGAAATTAGATCGAAAACCCATCAAGAAAAACTCCTCTGTGACCGTCCGGACCGACACCAACTGTCAGTTTCGCACCACGGATATGGACACCGATGGCGGCTCCTACCCTCGATGGAACGAGAAGCTCGTGCTCGATTTGCCAGAGCACTCGAGGTACCTCACGGTGGAGGTCCAATGCAAGACGTCGTACGCGGTCAGGACGGTCGGGACGGCGACGATTCCGGCGTCGGATTTTGTAGGTGGGTACGTGCCGGAGGGTTACCTGCATTTTTTGAGTTACAGGCTGAGGGATCACAGGGGGGAGAGGAATGGGATTATTAATATTTCTGTGAGAATGAAGGTTCCGGAGATGTATGCTTGCTCAAGTTCAACGACGTCGTCTCACTCGAGAATGGGGTTTCCGGGGGCTGAGAACAATAATTTTGGTGGCGGCGTTGCGACAGGAGTTCCGGTTTGGTATGGTGCTTACCAGAAAAAACATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

180

Amino Acids

19.97

Weight (kDa)

9.13

Isoelectric Point (pI)

31.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 7 - 103 4.3e-10 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 239, 431
Acc36I ACCTGC 1 cut(s) 324
Acc65I GGTACC 1 cut(s) 207
AccB1I GGYRCC 1 cut(s) 207
AccII CGCG 1 cut(s) 245
AccIII TCCGGA 2 cut(s) 99, 397
AciI CCGC 4 cut(s) 42, 150, 245, 483
AclWI GGATC 1 cut(s) 351
AcyI GRCGYC 3 cut(s) 236, 278, 428
AfaI GTAC 3 cut(s) 209, 242, 299
AfiI CCNNNNNNNGG 2 cut(s) 15, 217
AgsI TTSAA 1 cut(s) 423
AluBI AGCT 1 cut(s) 178
AluI AGCT 1 cut(s) 178
Alw21I GWGCWC 2 cut(s) 186, 201
Alw26I GTCTC 1 cut(s) 438
AlwI GGATC 1 cut(s) 351
Ama87I CYCGRG 3 cut(s) 23, 202, 439
Aor13HI TCCGGA 2 cut(s) 99, 397
ApeKI GCWGC 1 cut(s) 5
AseI ATTAAT 1 cut(s) 372
Asp718I GGTACC 1 cut(s) 207
AspS9I GGNCC 2 cut(s) 102, 223
AsuC2I CCSGG 2 cut(s) 16, 456
AvaI CYCGRG 3 cut(s) 23, 202, 439
AvaII GGWCC 2 cut(s) 102, 223
BanI GGYRCC 1 cut(s) 207
BauI CACGAG 1 cut(s) 179
Bbv12I GWGCWC 2 cut(s) 186, 201
BbvCI CCTCAGC 1 cut(s) 338
BbvI GCAGC 1 cut(s) 17
BccI CCATC 3 cut(s) 80, 140, 159
BceAI ACGGC 1 cut(s) 279
BcnI CCSGG 2 cut(s) 16, 456
BcoDI GTCTC 1 cut(s) 438
BfuAI ACCTGC 1 cut(s) 324
BisI GCNGC 3 cut(s) 6, 151, 484
BlsI GCNGC 3 cut(s) 7, 152, 485
Bme1390I CCNGG 2 cut(s) 16, 456
Bme18I GGWCC 2 cut(s) 102, 223
BmeT110I CYCGRG 3 cut(s) 23, 202, 439
BmgT120I GGNCC 2 cut(s) 102, 223
BmiI GGNNCC 3 cut(s) 154, 209, 396
BmrFI CCNGG 2 cut(s) 16, 456
Bpu10I CCTNAGC 1 cut(s) 338
BpuEI CTTGAG 1 cut(s) 400
BpuMI CCSGG 2 cut(s) 16, 456
BsaAI YACGTR 1 cut(s) 301
BsaHI GRCGYC 3 cut(s) 236, 278, 428
BsaJI CCNNGG 2 cut(s) 129, 455
BsaWI WCCGGW 3 cut(s) 99, 397, 502
BsaXI ACNNNNNCTCC 2 cut(s) 489, 519
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 217
BseAI TCCGGA 2 cut(s) 99, 397
BseDI CCNNGG 2 cut(s) 129, 455
BseLI CCNNNNNNNGG 2 cut(s) 15, 217
BseMII CTCAG 2 cut(s) 329, 453
BseRI GAGGAG 1 cut(s) 76
BseXI GCAGC 1 cut(s) 17
Bsh1236I CGCG 1 cut(s) 245
Bsh1285I CGRYCG 1 cut(s) 258
BshNI GGYRCC 1 cut(s) 207
BsiEI CGRYCG 1 cut(s) 258
BsiHKAI GWGCWC 2 cut(s) 186, 201
BsiHKCI CYCGRG 3 cut(s) 23, 202, 439
BsiSI CCGG 7 cut(s) 16, 100, 275, 305, 398, 455, 503
BsiWI CGTACG 1 cut(s) 240
BslFI GGGAC 1 cut(s) 274
BslI CCNNNNNNNGG 2 cut(s) 15, 217
BsmAI GTCTC 1 cut(s) 438
BsmBI CGTCTC 1 cut(s) 438
BsmFI GGGAC 1 cut(s) 274
BsoBI CYCGRG 3 cut(s) 23, 202, 439
Bsp1286I GDGCHC 2 cut(s) 186, 201
Bsp13I TCCGGA 2 cut(s) 99, 397
Bsp143I GATC 3 cut(s) 27, 61, 343
BspACI CCGC 4 cut(s) 42, 150, 245, 483
BspCNI CTCAG 2 cut(s) 330, 454
BspEI TCCGGA 2 cut(s) 99, 397
BspFNI CGCG 1 cut(s) 245
BspLI GGNNCC 3 cut(s) 154, 209, 396
BspMI ACCTGC 1 cut(s) 324
BspPI GGATC 1 cut(s) 351
BspT107I GGYRCC 1 cut(s) 207
BssECI CCNNGG 2 cut(s) 129, 455
BssMI GATC 3 cut(s) 27, 61, 343
BssNI GRCGYC 3 cut(s) 236, 278, 428
BssSI CACGAG 1 cut(s) 179
Bst2BI CACGAG 1 cut(s) 179
Bst4CI ACNGT 5 cut(s) 34, 97, 116, 217, 256
BstACI GRCGYC 3 cut(s) 236, 278, 428
BstBAI YACGTR 1 cut(s) 301
BstC8I GCNNGC 1 cut(s) 412
BstDEI CTNAG 2 cut(s) 338, 462
BstDSI CCRYGG 1 cut(s) 129
BstEII GGTNACC 1 cut(s) 311
BstFNI CGCG 1 cut(s) 245
BstKTI GATC 3 cut(s) 30, 64, 346
BstMAI GTCTC 1 cut(s) 438
BstMBI GATC 3 cut(s) 27, 61, 343
BstMCI CGRYCG 1 cut(s) 258
BstMWI GCNNNNNNNGC 1 cut(s) 190
BstPI GGTNACC 1 cut(s) 311
BstSCI CCNGG 2 cut(s) 14, 454
BstUI CGCG 1 cut(s) 245
BstV1I GCAGC 1 cut(s) 17
BstXI CCANNNNNNTGG 1 cut(s) 136
BtgI CCRYGG 1 cut(s) 129
BveI ACCTGC 1 cut(s) 324
Cac8I GCNNGC 1 cut(s) 412
Cfr13I GGNCC 2 cut(s) 102, 223
CpoI CGGWCCG 1 cut(s) 102
CseI GACGC 2 cut(s) 28, 267
Csp6I GTAC 3 cut(s) 208, 241, 298
CspI CGGWCCG 1 cut(s) 102
CviJI RGCY 5 cut(s) 8, 153, 178, 337, 461
CviKI_1 RGCY 5 cut(s) 8, 153, 178, 337, 461
CviQI GTAC 3 cut(s) 208, 241, 298
DdeI CTNAG 2 cut(s) 338, 462
DpnI GATC 3 cut(s) 29, 63, 345
DpnII GATC 3 cut(s) 27, 61, 343
EciI GGCGGA 1 cut(s) 31
Eco47I GGWCC 2 cut(s) 102, 223
Eco88I CYCGRG 3 cut(s) 23, 202, 439
Eco91I GGTNACC 1 cut(s) 311
EcoO65I GGTNACC 1 cut(s) 311
Esp3I CGTCTC 1 cut(s) 438
FaiI YATR 3 cut(s) 137, 408, 513
FaqI GGGAC 1 cut(s) 274
Fnu4HI GCNGC 3 cut(s) 6, 151, 484
Fsp4HI GCNGC 3 cut(s) 6, 151, 484
GluI GCNGC 3 cut(s) 6, 151, 484
HapII CCGG 7 cut(s) 16, 100, 275, 305, 398, 455, 503
HgaI GACGC 2 cut(s) 28, 267
Hin1I GRCGYC 3 cut(s) 236, 278, 428
HinfI GANTC 2 cut(s) 49, 271
HpaII CCGG 7 cut(s) 16, 100, 275, 305, 398, 455, 503
Hpy188I TCNGA 2 cut(s) 54, 283
Hpy188III TCNNGA 7 cut(s) 25, 76, 100, 250, 259, 398, 441
Hpy99I CGWCG 5 cut(s) 241, 271, 283, 430, 433
HpyAV CCTTC 1 cut(s) 385
HpyCH4III ACNGT 5 cut(s) 34, 97, 116, 217, 256
HpyCH4IV ACGT 3 cut(s) 236, 300, 428
HpyCH4V TGCA 2 cut(s) 231, 319
HpyF10VI GCNNNNNNNGC 1 cut(s) 190
HpyF3I CTNAG 2 cut(s) 338, 462
HpySE526I ACGT 3 cut(s) 236, 300, 428
Hsp92I GRCGYC 3 cut(s) 236, 278, 428
Kpn2I TCCGGA 2 cut(s) 99, 397
KpnI GGTACC 1 cut(s) 211
Kzo9I GATC 3 cut(s) 27, 61, 343
LmnI GCTCC 1 cut(s) 158
Lsp1109I GCAGC 1 cut(s) 17
MaeII ACGT 3 cut(s) 236, 300, 428
MaeIII GTNAC 3 cut(s) 91, 311, 329
MalI GATC 3 cut(s) 29, 63, 345
MboI GATC 3 cut(s) 27, 61, 343
MhlI GDGCHC 2 cut(s) 186, 201
MluCI AATT 3 cut(s) 10, 56, 472
MmeI TCCRAC 1 cut(s) 261
MnlI CCTC 8 cut(s) 97, 171, 198, 214, 221, 301, 333, 351
MroI TCCGGA 2 cut(s) 99, 397
MseI TTAA 1 cut(s) 372
MslI CAYNNNNRTG 1 cut(s) 134
MspI CCGG 7 cut(s) 16, 100, 275, 305, 398, 455, 503
MspR9I CCNGG 2 cut(s) 16, 456
MvnI CGCG 1 cut(s) 245
MwoI GCNNNNNNNGC 1 cut(s) 190
NciI CCSGG 2 cut(s) 16, 456
NdeII GATC 3 cut(s) 27, 61, 343
NlaIV GGNNCC 3 cut(s) 154, 209, 396
NmeAIII GCCGAG 1 cut(s) 70
NmuCI GTSAC 1 cut(s) 91
PaeR7I CTCGAG 3 cut(s) 23, 202, 439
PcsI WCGNNNNNNNCGW 3 cut(s) 177, 275, 437
PfeI GAWTC 2 cut(s) 49, 271
Pfl23II CGTACG 1 cut(s) 240
PflFI GACNNNGTC 1 cut(s) 430
PfoI TCCNGGA 1 cut(s) 14
PkrI GCNGC 3 cut(s) 7, 152, 485
Ppu21I YACGTR 1 cut(s) 301
PshBI ATTAAT 1 cut(s) 372
PspEI GGTNACC 1 cut(s) 311
PspLI CGTACG 1 cut(s) 240
PspN4I GGNNCC 3 cut(s) 154, 209, 396
PspPI GGNCC 2 cut(s) 102, 223
PspXI VCTCGAGB 1 cut(s) 202
PsyI GACNNNGTC 1 cut(s) 430
RsaI GTAC 3 cut(s) 209, 242, 299
RsaNI GTAC 3 cut(s) 208, 241, 298
RseI CAYNNNNRTG 1 cut(s) 134
Rsr2I CGGWCCG 1 cut(s) 102
RsrII CGGWCCG 1 cut(s) 102
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 3 cut(s) 6, 151, 484
Sau3AI GATC 3 cut(s) 27, 61, 343
Sau96I GGNCC 2 cut(s) 102, 223
ScrFI CCNGG 2 cut(s) 16, 456
SduI GDGCHC 2 cut(s) 186, 201
Sfr274I CTCGAG 3 cut(s) 23, 202, 439
SinI GGWCC 2 cut(s) 102, 223
SlaI CTCGAG 3 cut(s) 23, 202, 439
SmiMI CAYNNNNRTG 1 cut(s) 134
SmlI CTYRAG 4 cut(s) 23, 202, 415, 439
SmoI CTYRAG 4 cut(s) 23, 202, 415, 439
Sse9I AATT 3 cut(s) 10, 56, 472
SsiI CCGC 4 cut(s) 42, 150, 245, 483
SspI AATATT 1 cut(s) 376
StyD4I CCNGG 2 cut(s) 14, 454
TaaI ACNGT 5 cut(s) 34, 97, 116, 217, 256
TaiI ACGT 3 cut(s) 239, 303, 431
TaqI TCGA 6 cut(s) 24, 64, 163, 186, 203, 440
TaqII GACCGA 1 cut(s) 119
TasI AATT 3 cut(s) 10, 56, 472
TauI GCSGC 2 cut(s) 153, 486
TfiI GAWTC 2 cut(s) 49, 271
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 1 cut(s) 404
TspGWI ACGGA 1 cut(s) 146
Tth111I GACNNNGTC 1 cut(s) 430
VpaK11BI GGWCC 2 cut(s) 102, 223
VspI ATTAAT 1 cut(s) 372
XhoI CTCGAG 3 cut(s) 23, 202, 439
ZraI GACGTC 2 cut(s) 237, 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.