MD01G1049600.v1.1

BON1-associated protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002449
Physical Location & Seq
Forward (+)
15238803 .. 15239339
537 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1049600.v1.1.491

Sequence Viewer

Length: 537 bp
ATGACACCCAGTTCCCGGACCCTCGAGATCACGGTCATCTCCGCCGAGAATCTGAAATTAGATCGAAAACCCATCAAGAAAAACGCCTCCGTCACCGTCCGGACCGACACCAACAGTCAGTTTCGCACCACTGAAATGGACACTGAAGGCGGCTCCTACCCTCGATGGAACGAGAAGCTCGTGCTCGACATGCCAACTCACTCGAGGTCCATCACGGTGGAGGTCCAATGCAAGACGTCGTACGGCGTCAGGACGATCGGGACGGCGTCGATTCCGGCGTCGGATTTTGTAGGTGGGTACGTGCCGGAGGGCTACCTGCATTTTTTGAGTTACAGGCTGAGGGATCACATGGGGGAGAGGAATGGGATTATTAATATCTCTGTGAGAATGAAGGTTCCGGAGATGTGCGCTTGCTCAAGTTCAACGACGTCGTCTCACTCGAAAATGGGGTTTCCGGCGGCTGGGAATAATAATTCTGGCGGCGGCGTTGCGATAGGAGTTCCCGTTTGGTACGGTGCTTATCAGAAAAATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

179

Amino Acids

19.52

Weight (kDa)

9.17

Isoelectric Point (pI)

36.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 7 - 103 3.4e-10 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 239, 431
Acc36I ACCTGC 1 cut(s) 324
AccIII TCCGGA 2 cut(s) 99, 397
AciI CCGC 5 cut(s) 42, 150, 458, 480, 483
AclWI GGATC 1 cut(s) 351
AcuI CTGAAG 1 cut(s) 165
AcyI GRCGYC 5 cut(s) 236, 246, 266, 278, 428
AfaI GTAC 3 cut(s) 242, 299, 512
AfiI CCNNNNNNNGG 2 cut(s) 15, 461
AgsI TTSAA 1 cut(s) 423
AluBI AGCT 1 cut(s) 178
AluI AGCT 1 cut(s) 178
Alw21I GWGCWC 1 cut(s) 186
Alw26I GTCTC 1 cut(s) 438
AlwI GGATC 1 cut(s) 351
Ama87I CYCGRG 2 cut(s) 23, 202
Aor13HI TCCGGA 2 cut(s) 99, 397
AseI ATTAAT 1 cut(s) 372
AspLEI GCGC 1 cut(s) 410
AspS9I GGNCC 4 cut(s) 18, 102, 207, 223
AsuC2I CCSGG 1 cut(s) 16
AsuHPI GGTGA 1 cut(s) 85
AvaI CYCGRG 2 cut(s) 23, 202
AvaII GGWCC 4 cut(s) 18, 102, 207, 223
BauI CACGAG 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 186
BbvCI CCTCAGC 1 cut(s) 338
BccI CCATC 3 cut(s) 80, 159, 218
BceAI ACGGC 2 cut(s) 259, 279
BcnI CCSGG 1 cut(s) 16
BcoDI GTCTC 1 cut(s) 438
BfuAI ACCTGC 1 cut(s) 324
BisI GCNGC 4 cut(s) 151, 459, 481, 484
BlsI GCNGC 4 cut(s) 152, 460, 482, 485
Bme1390I CCNGG 1 cut(s) 16
Bme18I GGWCC 4 cut(s) 18, 102, 207, 223
BmeT110I CYCGRG 2 cut(s) 23, 202
BmgT120I GGNCC 4 cut(s) 18, 102, 207, 223
BmiI GGNNCC 3 cut(s) 20, 154, 396
BmrFI CCNGG 1 cut(s) 16
BmrI ACTGGG 1 cut(s) 3
BmuI ACTGGG 1 cut(s) 3
Bpu10I CCTNAGC 1 cut(s) 338
BpuEI CTTGAG 1 cut(s) 400
BpuMI CCSGG 1 cut(s) 16
BsaAI YACGTR 1 cut(s) 301
BsaHI GRCGYC 5 cut(s) 236, 246, 266, 278, 428
BsaWI WCCGGW 2 cut(s) 99, 397
BsaXI ACNNNNNCTCC 2 cut(s) 489, 519
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 461
Bse1I ACTGG 1 cut(s) 9
BseAI TCCGGA 2 cut(s) 99, 397
BseLI CCNNNNNNNGG 2 cut(s) 15, 461
BseMII CTCAG 1 cut(s) 329
BseNI ACTGG 1 cut(s) 9
BseYI CCCAGC 1 cut(s) 461
Bsh1285I CGRYCG 1 cut(s) 258
BsiEI CGRYCG 1 cut(s) 258
BsiHKAI GWGCWC 1 cut(s) 186
BsiHKCI CYCGRG 2 cut(s) 23, 202
BsiSI CCGG 6 cut(s) 16, 100, 275, 305, 398, 455
BsiWI CGTACG 1 cut(s) 240
BslFI GGGAC 1 cut(s) 274
BslI CCNNNNNNNGG 2 cut(s) 15, 461
BsmAI GTCTC 1 cut(s) 438
BsmBI CGTCTC 1 cut(s) 438
BsmFI GGGAC 1 cut(s) 274
BsoBI CYCGRG 2 cut(s) 23, 202
Bsp1286I GDGCHC 1 cut(s) 186
Bsp13I TCCGGA 2 cut(s) 99, 397
Bsp143I GATC 4 cut(s) 27, 61, 255, 343
BspACI CCGC 5 cut(s) 42, 150, 458, 480, 483
BspCNI CTCAG 1 cut(s) 330
BspEI TCCGGA 2 cut(s) 99, 397
BspLI GGNNCC 3 cut(s) 20, 154, 396
BspMI ACCTGC 1 cut(s) 324
BspPI GGATC 1 cut(s) 351
BsrI ACTGG 1 cut(s) 9
BssMI GATC 4 cut(s) 27, 61, 255, 343
BssNI GRCGYC 5 cut(s) 236, 246, 266, 278, 428
BssSI CACGAG 1 cut(s) 179
Bst2BI CACGAG 1 cut(s) 179
Bst4CI ACNGT 5 cut(s) 34, 97, 116, 217, 515
BstACI GRCGYC 5 cut(s) 236, 246, 266, 278, 428
BstBAI YACGTR 1 cut(s) 301
BstC8I GCNNGC 1 cut(s) 412
BstDEI CTNAG 1 cut(s) 338
BstHHI GCGC 1 cut(s) 410
BstKTI GATC 4 cut(s) 30, 64, 258, 346
BstMAI GTCTC 1 cut(s) 438
BstMBI GATC 4 cut(s) 27, 61, 255, 343
BstMCI CGRYCG 1 cut(s) 258
BstMWI GCNNNNNNNGC 1 cut(s) 190
BstNSI RCATGY 1 cut(s) 193
BstSCI CCNGG 1 cut(s) 14
BstXI CCANNNNNNTGG 2 cut(s) 136, 217
BtsIMutI CAGTG 2 cut(s) 129, 141
BveI ACCTGC 1 cut(s) 324
Cac8I GCNNGC 1 cut(s) 412
CfoI GCGC 1 cut(s) 410
Cfr13I GGNCC 4 cut(s) 18, 102, 207, 223
CpoI CGGWCCG 1 cut(s) 102
CseI GACGC 3 cut(s) 235, 255, 267
Csp6I GTAC 3 cut(s) 241, 298, 511
CspI CGGWCCG 1 cut(s) 102
CviAII CATG 2 cut(s) 190, 349
CviJI RGCY 5 cut(s) 153, 178, 312, 337, 461
CviKI_1 RGCY 5 cut(s) 153, 178, 312, 337, 461
CviQI GTAC 3 cut(s) 241, 298, 511
DdeI CTNAG 1 cut(s) 338
DpnI GATC 4 cut(s) 29, 63, 257, 345
DpnII GATC 4 cut(s) 27, 61, 255, 343
EciI GGCGGA 1 cut(s) 31
Eco47I GGWCC 4 cut(s) 18, 102, 207, 223
Eco57I CTGAAG 1 cut(s) 165
Eco88I CYCGRG 2 cut(s) 23, 202
Esp3I CGTCTC 1 cut(s) 438
FaeI CATG 2 cut(s) 193, 352
FaiI YATR 2 cut(s) 191, 350
FaqI GGGAC 1 cut(s) 274
FatI CATG 2 cut(s) 189, 348
Fnu4HI GCNGC 4 cut(s) 151, 459, 481, 484
Fsp4HI GCNGC 4 cut(s) 151, 459, 481, 484
GlaI GCGC 1 cut(s) 409
GluI GCNGC 4 cut(s) 151, 459, 481, 484
GsaI CCCAGC 1 cut(s) 465
HapII CCGG 6 cut(s) 16, 100, 275, 305, 398, 455
HgaI GACGC 3 cut(s) 235, 255, 267
HhaI GCGC 1 cut(s) 410
Hin1I GRCGYC 5 cut(s) 236, 246, 266, 278, 428
Hin1II CATG 2 cut(s) 193, 352
Hin6I GCGC 1 cut(s) 408
HinP1I GCGC 1 cut(s) 408
HinfI GANTC 2 cut(s) 49, 271
HpaII CCGG 6 cut(s) 16, 100, 275, 305, 398, 455
HphI GGTGA 1 cut(s) 85
Hpy188I TCNGA 3 cut(s) 54, 283, 525
Hpy188III TCNNGA 6 cut(s) 25, 76, 100, 250, 259, 398
Hpy99I CGWCG 5 cut(s) 241, 271, 283, 430, 433
HpyAV CCTTC 2 cut(s) 140, 385
HpyCH4III ACNGT 5 cut(s) 34, 97, 116, 217, 515
HpyCH4IV ACGT 3 cut(s) 236, 300, 428
HpyCH4V TGCA 2 cut(s) 231, 319
HpyF10VI GCNNNNNNNGC 1 cut(s) 190
HpyF3I CTNAG 1 cut(s) 338
HpySE526I ACGT 3 cut(s) 236, 300, 428
Hsp92I GRCGYC 5 cut(s) 236, 246, 266, 278, 428
Hsp92II CATG 2 cut(s) 193, 352
HspAI GCGC 1 cut(s) 408
Kpn2I TCCGGA 2 cut(s) 99, 397
Kzo9I GATC 4 cut(s) 27, 61, 255, 343
LmnI GCTCC 1 cut(s) 158
MaeII ACGT 3 cut(s) 236, 300, 428
MaeIII GTNAC 2 cut(s) 91, 329
MalI GATC 4 cut(s) 29, 63, 257, 345
MboI GATC 4 cut(s) 27, 61, 255, 343
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 3 cut(s) 56, 472, 529
MmeI TCCRAC 1 cut(s) 261
MnlI CCTC 8 cut(s) 32, 97, 171, 198, 214, 301, 333, 351
MroI TCCGGA 2 cut(s) 99, 397
MseI TTAA 1 cut(s) 372
MslI CAYNNNNRTG 2 cut(s) 134, 215
MspI CCGG 6 cut(s) 16, 100, 275, 305, 398, 455
MspR9I CCNGG 1 cut(s) 16
MwoI GCNNNNNNNGC 1 cut(s) 190
NciI CCSGG 1 cut(s) 16
NdeII GATC 4 cut(s) 27, 61, 255, 343
NlaIII CATG 2 cut(s) 193, 352
NlaIV GGNNCC 3 cut(s) 20, 154, 396
NmeAIII GCCGAG 1 cut(s) 70
NmuCI GTSAC 1 cut(s) 91
NspI RCATGY 1 cut(s) 193
PaeR7I CTCGAG 2 cut(s) 23, 202
PcsI WCGNNNNNNNCGW 3 cut(s) 177, 275, 437
PfeI GAWTC 2 cut(s) 49, 271
Pfl23II CGTACG 1 cut(s) 240
PflFI GACNNNGTC 2 cut(s) 265, 430
PfoI TCCNGGA 1 cut(s) 14
PkrI GCNGC 4 cut(s) 152, 460, 482, 485
Ple19I CGATCG 1 cut(s) 258
Ppu21I YACGTR 1 cut(s) 301
PshBI ATTAAT 1 cut(s) 372
PspFI CCCAGC 1 cut(s) 461
PspLI CGTACG 1 cut(s) 240
PspN4I GGNNCC 3 cut(s) 20, 154, 396
PspPI GGNCC 4 cut(s) 18, 102, 207, 223
PspXI VCTCGAGB 1 cut(s) 202
PsyI GACNNNGTC 2 cut(s) 265, 430
PvuI CGATCG 1 cut(s) 258
RsaI GTAC 3 cut(s) 242, 299, 512
RsaNI GTAC 3 cut(s) 241, 298, 511
RseI CAYNNNNRTG 2 cut(s) 134, 215
Rsr2I CGGWCCG 1 cut(s) 102
RsrII CGGWCCG 1 cut(s) 102
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 4 cut(s) 151, 459, 481, 484
Sau3AI GATC 4 cut(s) 27, 61, 255, 343
Sau96I GGNCC 4 cut(s) 18, 102, 207, 223
ScrFI CCNGG 1 cut(s) 16
SduI GDGCHC 1 cut(s) 186
SetI ASST 9 cut(s) 180, 209, 225, 239, 295, 303, 318, 396, 431
Sfr274I CTCGAG 2 cut(s) 23, 202
SinI GGWCC 4 cut(s) 18, 102, 207, 223
SlaI CTCGAG 2 cut(s) 23, 202
SmiMI CAYNNNNRTG 2 cut(s) 134, 215
SmlI CTYRAG 3 cut(s) 23, 202, 415
SmoI CTYRAG 3 cut(s) 23, 202, 415
Sse9I AATT 3 cut(s) 56, 472, 529
SsiI CCGC 5 cut(s) 42, 150, 458, 480, 483
StyD4I CCNGG 1 cut(s) 14
TaaI ACNGT 5 cut(s) 34, 97, 116, 217, 515
TaiI ACGT 3 cut(s) 239, 303, 431
TaqI TCGA 7 cut(s) 24, 64, 163, 186, 203, 269, 440
TaqII GACCGA 1 cut(s) 119
TasI AATT 3 cut(s) 56, 472, 529
TauI GCSGC 4 cut(s) 153, 461, 483, 486
TfiI GAWTC 2 cut(s) 49, 271
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TscAI CASTG 2 cut(s) 136, 148
TseFI GTSAC 1 cut(s) 91
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 1 cut(s) 404
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 2 cut(s) 136, 148
Tth111I GACNNNGTC 2 cut(s) 265, 430
VpaK11BI GGWCC 4 cut(s) 18, 102, 207, 223
VspI ATTAAT 1 cut(s) 372
XceI RCATGY 1 cut(s) 193
XhoI CTCGAG 2 cut(s) 23, 202
ZraI GACGTC 2 cut(s) 237, 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.