MD07G1126600.v1.1

BON1-associated protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002449
Physical Location & Seq
Forward (+)
17871046 .. 17871606
561 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1126600.v1.1.491

Sequence Viewer

Length: 561 bp
ATGGCAGCCAATTTTCGAACGCTTGAAATCACAGTGATCTCCCTCGAGAACCTACAATTAGATCAAAAACCCATTAAGACAAATGCCTCAGTGACCGTCCGGATCGACACCAACAGCCAGTTTCGCACCACAATTATAGACACCGAAGGCGACGCCTACCCACTATGGAACGAGAAGCTTGTGCTCGAATTGCCAATGCACTCCAAGTCCACCACGGTGGGGGTCCAATGCAAGACCGCGTACGGCGTCAGGATGTTCGGAACGGCAACTGTTTTGGCATCTGATTTTGTTGGTGCTTACGTGCCGGAGAGTTACCTGCATTTTTTGAGCTACAGGCTAAGAGATTACAAGGGGGAGAGGAATGGGGTTATTAATATTTCTGTGAGGATGAGGGTTCCGGAGATGTATGCTTGTGCAAGTTCAACAACGTCTTCTCACTCAAGGATGGGGTTTCCGACAGCTGGGAACCATAGTTTTGGTGGCGGCGTTGCGATCGGAGTTCCAGTTTGGTATGGTGCCTATCAGAAAAATTATTCACATTATTTTGTGCGTAAATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

187

Amino Acids

20.81

Weight (kDa)

9.2

Isoelectric Point (pI)

31.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 7 - 78 1.8e-06 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 324
AccB1I GGYRCC 1 cut(s) 515
AccII CGCG 1 cut(s) 239
AccIII TCCGGA 2 cut(s) 99, 397
AciI CCGC 2 cut(s) 237, 483
AclWI GGATC 1 cut(s) 110
AcyI GRCGYC 2 cut(s) 153, 246
AfaI GTAC 1 cut(s) 242
AfiI CCNNNNNNNGG 2 cut(s) 219, 461
AgsI TTSAA 2 cut(s) 26, 423
AleI CACNNNNGTG 1 cut(s) 215
AluBI AGCT 3 cut(s) 178, 330, 461
AluI AGCT 3 cut(s) 178, 330, 461
Alw21I GWGCWC 1 cut(s) 186
AlwI GGATC 1 cut(s) 110
Ama87I CYCGRG 1 cut(s) 44
Aor13HI TCCGGA 2 cut(s) 99, 397
ApeKI GCWGC 1 cut(s) 5
AseI ATTAAT 1 cut(s) 372
AspS9I GGNCC 1 cut(s) 223
AsuII TTCGAA 1 cut(s) 16
AvaI CYCGRG 1 cut(s) 44
AvaII GGWCC 1 cut(s) 223
BanI GGYRCC 1 cut(s) 515
BbsI GAAGAC 1 cut(s) 423
Bbv12I GWGCWC 1 cut(s) 186
BbvI GCAGC 1 cut(s) 17
BccI CCATC 1 cut(s) 439
BceAI ACGGC 2 cut(s) 259, 279
BfmI CTRYAG 1 cut(s) 331
BfuAI ACCTGC 1 cut(s) 324
BisI GCNGC 2 cut(s) 6, 484
BlsI GCNGC 2 cut(s) 7, 485
Bme18I GGWCC 1 cut(s) 223
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 223
BmiI GGNNCC 4 cut(s) 224, 396, 467, 517
BmsI GCATC 1 cut(s) 287
BpiI GAAGAC 1 cut(s) 423
Bpu14I TTCGAA 1 cut(s) 16
BpuEI CTTGAG 1 cut(s) 424
BsaAI YACGTR 1 cut(s) 301
BsaHI GRCGYC 2 cut(s) 153, 246
BsaJI CCNNGG 1 cut(s) 213
BsaWI WCCGGW 2 cut(s) 99, 397
BsaXI ACNNNNNCTCC 2 cut(s) 489, 519
Bsc4I CCNNNNNNNGG 2 cut(s) 219, 461
Bse1I ACTGG 2 cut(s) 118, 503
BseAI TCCGGA 2 cut(s) 99, 397
BseDI CCNNGG 1 cut(s) 213
BseGI GGATG 3 cut(s) 258, 393, 450
BseLI CCNNNNNNNGG 2 cut(s) 219, 461
BseMII CTCAG 1 cut(s) 102
BseNI ACTGG 2 cut(s) 118, 503
BseXI GCAGC 1 cut(s) 17
BseYI CCCAGC 1 cut(s) 461
Bsh1236I CGCG 1 cut(s) 239
Bsh1285I CGRYCG 1 cut(s) 495
BshNI GGYRCC 1 cut(s) 515
BsiEI CGRYCG 1 cut(s) 495
BsiHKAI GWGCWC 1 cut(s) 186
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 3 cut(s) 100, 305, 398
BsiWI CGTACG 1 cut(s) 240
BslI CCNNNNNNNGG 2 cut(s) 219, 461
BsoBI CYCGRG 1 cut(s) 44
Bsp119I TTCGAA 1 cut(s) 16
Bsp1286I GDGCHC 1 cut(s) 186
Bsp13I TCCGGA 2 cut(s) 99, 397
Bsp143I GATC 4 cut(s) 36, 61, 102, 492
BspACI CCGC 2 cut(s) 237, 483
BspCNI CTCAG 1 cut(s) 101
BspEI TCCGGA 2 cut(s) 99, 397
BspFNI CGCG 1 cut(s) 239
BspLI GGNNCC 4 cut(s) 224, 396, 467, 517
BspMI ACCTGC 1 cut(s) 324
BspPI GGATC 1 cut(s) 110
BspT104I TTCGAA 1 cut(s) 16
BspT107I GGYRCC 1 cut(s) 515
BsrI ACTGG 2 cut(s) 118, 503
BssECI CCNNGG 1 cut(s) 213
BssMI GATC 4 cut(s) 36, 61, 102, 492
BssNI GRCGYC 2 cut(s) 153, 246
Bst4CI ACNGT 4 cut(s) 34, 97, 217, 271
BstACI GRCGYC 2 cut(s) 153, 246
BstBAI YACGTR 1 cut(s) 301
BstBI TTCGAA 1 cut(s) 16
BstDEI CTNAG 2 cut(s) 88, 338
BstDSI CCRYGG 1 cut(s) 213
BstF5I GGATG 3 cut(s) 258, 393, 450
BstFNI CGCG 1 cut(s) 239
BstKTI GATC 4 cut(s) 39, 64, 105, 495
BstMBI GATC 4 cut(s) 36, 61, 102, 492
BstMCI CGRYCG 1 cut(s) 495
BstMWI GCNNNNNNNGC 2 cut(s) 123, 190
BstSFI CTRYAG 1 cut(s) 331
BstUI CGCG 1 cut(s) 239
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 1 cut(s) 423
BstXI CCANNNNNNTGG 2 cut(s) 217, 476
BtgI CCRYGG 1 cut(s) 213
BtsCI GGATG 3 cut(s) 258, 393, 450
BtsIMutI CAGTG 2 cut(s) 39, 96
BveI ACCTGC 1 cut(s) 324
Cfr13I GGNCC 1 cut(s) 223
CseI GACGC 2 cut(s) 161, 235
Csp6I GTAC 1 cut(s) 241
CviJI RGCY 6 cut(s) 8, 117, 178, 330, 337, 461
CviKI_1 RGCY 6 cut(s) 8, 117, 178, 330, 337, 461
CviQI GTAC 1 cut(s) 241
DdeI CTNAG 2 cut(s) 88, 338
DpnI GATC 4 cut(s) 38, 63, 104, 494
DpnII GATC 4 cut(s) 36, 61, 102, 492
Eco47I GGWCC 1 cut(s) 223
Eco88I CYCGRG 1 cut(s) 44
FaiI YATR 5 cut(s) 137, 166, 408, 471, 513
Fnu4HI GCNGC 2 cut(s) 6, 484
FokI GGATG 3 cut(s) 265, 400, 457
Fsp4HI GCNGC 2 cut(s) 6, 484
GluI GCNGC 2 cut(s) 6, 484
GsaI CCCAGC 1 cut(s) 465
HapII CCGG 3 cut(s) 100, 305, 398
HgaI GACGC 2 cut(s) 161, 235
Hin1I GRCGYC 2 cut(s) 153, 246
HindIII AAGCTT 1 cut(s) 176
HpaII CCGG 3 cut(s) 100, 305, 398
Hpy166II GTNNAC 1 cut(s) 210
Hpy188I TCNGA 5 cut(s) 260, 283, 456, 497, 525
Hpy188III TCNNGA 4 cut(s) 46, 100, 250, 398
Hpy8I GTNNAC 1 cut(s) 210
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 1 cut(s) 140
HpyCH4III ACNGT 4 cut(s) 34, 97, 217, 271
HpyCH4IV ACGT 2 cut(s) 300, 428
HpyCH4V TGCA 4 cut(s) 199, 231, 319, 416
HpyF10VI GCNNNNNNNGC 2 cut(s) 123, 190
HpyF3I CTNAG 2 cut(s) 88, 338
HpySE526I ACGT 2 cut(s) 300, 428
Hsp92I GRCGYC 2 cut(s) 153, 246
Kpn2I TCCGGA 2 cut(s) 99, 397
Kzo9I GATC 4 cut(s) 36, 61, 102, 492
LpnPI CCDG 9 cut(s) 113, 131, 235, 318, 319, 329, 411, 447, 516
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 287
MaeII ACGT 2 cut(s) 300, 428
MaeIII GTNAC 2 cut(s) 91, 311
MalI GATC 4 cut(s) 38, 63, 104, 494
MboI GATC 4 cut(s) 36, 61, 102, 492
MboII GAAGA 1 cut(s) 423
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 5 cut(s) 10, 56, 132, 188, 529
MmeI TCCRAC 1 cut(s) 479
MnlI CCTC 5 cut(s) 53, 97, 351, 378, 384
MroI TCCGGA 2 cut(s) 99, 397
MseI TTAA 2 cut(s) 75, 372
MslI CAYNNNNRTG 1 cut(s) 215
MspA1I CMGCKG 1 cut(s) 461
MspI CCGG 3 cut(s) 100, 305, 398
MvnI CGCG 1 cut(s) 239
MwoI GCNNNNNNNGC 2 cut(s) 123, 190
NdeII GATC 4 cut(s) 36, 61, 102, 492
NlaIV GGNNCC 4 cut(s) 224, 396, 467, 517
NmuCI GTSAC 1 cut(s) 91
NspV TTCGAA 1 cut(s) 16
OliI CACNNNNGTG 1 cut(s) 215
PaeR7I CTCGAG 1 cut(s) 44
Pfl23II CGTACG 1 cut(s) 240
PkrI GCNGC 2 cut(s) 7, 485
Ple19I CGATCG 1 cut(s) 495
Ppu21I YACGTR 1 cut(s) 301
PshBI ATTAAT 1 cut(s) 372
PspFI CCCAGC 1 cut(s) 461
PspLI CGTACG 1 cut(s) 240
PspN4I GGNNCC 4 cut(s) 224, 396, 467, 517
PspPI GGNCC 1 cut(s) 223
PvuI CGATCG 1 cut(s) 495
PvuII CAGCTG 1 cut(s) 461
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
RseI CAYNNNNRTG 1 cut(s) 215
SaqAI TTAA 2 cut(s) 75, 372
SatI GCNGC 2 cut(s) 6, 484
Sau3AI GATC 4 cut(s) 36, 61, 102, 492
Sau96I GGNCC 1 cut(s) 223
SduI GDGCHC 1 cut(s) 186
SetI ASST 7 cut(s) 54, 180, 303, 318, 332, 431, 463
SfaNI GCATC 1 cut(s) 287
SfcI CTRYAG 1 cut(s) 331
Sfr274I CTCGAG 1 cut(s) 44
SfuI TTCGAA 1 cut(s) 16
SinI GGWCC 1 cut(s) 223
SlaI CTCGAG 1 cut(s) 44
SmiMI CAYNNNNRTG 1 cut(s) 215
SmlI CTYRAG 2 cut(s) 44, 439
SmoI CTYRAG 2 cut(s) 44, 439
Sse9I AATT 5 cut(s) 10, 56, 132, 188, 529
SsiI CCGC 2 cut(s) 237, 483
SspI AATATT 2 cut(s) 376, 557
TaaI ACNGT 4 cut(s) 34, 97, 217, 271
TaiI ACGT 2 cut(s) 303, 431
TaqI TCGA 4 cut(s) 16, 45, 105, 186
TasI AATT 5 cut(s) 10, 56, 132, 188, 529
TauI GCSGC 1 cut(s) 486
Tru1I TTAA 2 cut(s) 75, 372
Tru9I TTAA 2 cut(s) 75, 372
TscAI CASTG 2 cut(s) 39, 96
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 91
TspRI CASTG 2 cut(s) 39, 96
VpaK11BI GGWCC 1 cut(s) 223
VspI ATTAAT 1 cut(s) 372
XcmI CCANNNNNNNNNTGG 1 cut(s) 476
XhoI CTCGAG 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.