Rroxscaffold_4G00302410

BON1-associated protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002449
Physical Location & Seq
Forward (+)
22808422 .. 22808970
549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00302410.1

Sequence Viewer

Length: 549 bp
ATGGCAACTCGATATATAGAACTGACCGTTATCGCAGCCGAAAACCTCCAATCCAATCGCAAACCCTTGAAGAAAAACTCCTTCGTCACAGTCCGGACCGACGCCACCGTCACCGCCTCCAAATCGTACCGGACGGCGGAGACGGACTCCGAAGGCGGAGGGCCAAGATGGAATGAGAAGTTAGTGATCGAGGTTCCTGCGCAAGCCAGGAATCTGATCATGGAAGTGCAATGCCAGAACGGGGCGAGGACGATCGGAGCGGCGACTATTCCGTTGACGGACTTCATCGGCGGGTGGGTGCCCGAGAGCTACTTGCATTTTCTGAGTTACAGGCTGAGGGATGAGAGAGGTGTCAGGAATGGGATTATGAATATCTCGGTGAGGATGAAGGTGGACGAAGGTGCGACGTCGACGTACGCGAGGAAGTTGTCGAGTGGTTGCGCTACGACGTCGTCGCAGACCAGGTTGGGGTATCCAGCGGTGGATAACAGTGGCGTCGTAACTGGGGTTCCGCTGAATTGGAATAGCTATTCTCGAGTTCAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

182

Amino Acids

20.06

Weight (kDa)

9.48

Isoelectric Point (pI)

30.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 5 - 95 4.7e-08 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 107
AatII GACGTC 2 cut(s) 410, 452
Acc16I TGCGCA 1 cut(s) 201
AccB1I GGYRCC 1 cut(s) 298
AccBSI CCGCTC 1 cut(s) 260
AccI GTMKAC 1 cut(s) 410
AccII CGCG 1 cut(s) 419
AccIII TCCGGA 1 cut(s) 93
AciI CCGC 7 cut(s) 114, 137, 156, 260, 291, 479, 512
AcyI GRCGYC 4 cut(s) 102, 407, 449, 495
AfaI GTAC 2 cut(s) 128, 416
AfiI CCNNNNNNNGG 3 cut(s) 136, 241, 468
AgsI TTSAA 2 cut(s) 70, 542
AjnI CCWGG 2 cut(s) 206, 461
AluBI AGCT 2 cut(s) 309, 528
AluI AGCT 2 cut(s) 309, 528
Alw26I GTCTC 1 cut(s) 134
Ama87I CYCGRG 2 cut(s) 302, 534
Aor13HI TCCGGA 1 cut(s) 93
AoxI GGCC 1 cut(s) 161
ApeKI GCWGC 1 cut(s) 35
AspLEI GCGC 2 cut(s) 202, 443
AspS9I GGNCC 2 cut(s) 96, 161
AsuHPI GGTGA 2 cut(s) 103, 391
AvaI CYCGRG 2 cut(s) 302, 534
AvaII GGWCC 1 cut(s) 96
BaeGI GKGCMC 1 cut(s) 303
BanI GGYRCC 1 cut(s) 298
BbvCI CCTCAGC 1 cut(s) 335
BbvI GCAGC 1 cut(s) 47
BccI CCATC 1 cut(s) 162
BceAI ACGGC 1 cut(s) 150
BcgI CGANNNNNNTGC 2 cut(s) 179, 213
BciT130I CCWGG 2 cut(s) 208, 463
BciVI GTATCC 1 cut(s) 483
BclI TGATCA 1 cut(s) 216
BcoDI GTCTC 1 cut(s) 134
BfuI GTATCC 1 cut(s) 483
BisI GCNGC 2 cut(s) 36, 261
BlsI GCNGC 2 cut(s) 37, 262
Bme1390I CCNGG 2 cut(s) 208, 463
Bme18I GGWCC 1 cut(s) 96
BmeT110I CYCGRG 2 cut(s) 302, 534
BmgT120I GGNCC 2 cut(s) 96, 161
BmiI GGNNCC 3 cut(s) 195, 300, 510
BmrFI CCNGG 2 cut(s) 208, 463
BmrI ACTGGG 1 cut(s) 513
BmuI ACTGGG 1 cut(s) 513
BplI GAGNNNNNCTC 2 cut(s) 131, 163
Bpu10I CCTNAGC 1 cut(s) 335
BsaHI GRCGYC 4 cut(s) 102, 407, 449, 495
BsaWI WCCGGW 2 cut(s) 93, 129
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 241, 468
Bse1I ACTGG 1 cut(s) 508
Bse3DI GCAATG 1 cut(s) 236
BseAI TCCGGA 1 cut(s) 93
BseBI CCWGG 2 cut(s) 208, 463
BseGI GGATG 2 cut(s) 346, 390
BseLI CCNNNNNNNGG 3 cut(s) 136, 241, 468
BseMI GCAATG 1 cut(s) 236
BseMII CTCAG 2 cut(s) 314, 326
BseNI ACTGG 1 cut(s) 508
BseSI GKGCMC 1 cut(s) 303
BseXI GCAGC 1 cut(s) 47
Bsh1236I CGCG 1 cut(s) 419
Bsh1285I CGRYCG 1 cut(s) 255
BshFI GGCC 1 cut(s) 163
BshNI GGYRCC 1 cut(s) 298
BsiEI CGRYCG 1 cut(s) 255
BsiHKCI CYCGRG 2 cut(s) 302, 534
BsiSI CCGG 2 cut(s) 94, 130
BsiWI CGTACG 1 cut(s) 414
BslI CCNNNNNNNGG 3 cut(s) 136, 241, 468
BsmAI GTCTC 1 cut(s) 134
BsmBI CGTCTC 1 cut(s) 134
BsnI GGCC 1 cut(s) 163
BsoBI CYCGRG 2 cut(s) 302, 534
Bsp1286I GDGCHC 1 cut(s) 303
Bsp13I TCCGGA 1 cut(s) 93
Bsp143I GATC 3 cut(s) 186, 216, 252
BspACI CCGC 7 cut(s) 114, 137, 156, 260, 291, 479, 512
BspANI GGCC 1 cut(s) 163
BspCNI CTCAG 2 cut(s) 315, 327
BspEI TCCGGA 1 cut(s) 93
BspFNI CGCG 1 cut(s) 419
BspLI GGNNCC 3 cut(s) 195, 300, 510
BspT107I GGYRCC 1 cut(s) 298
BsrBI CCGCTC 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 236
BsrI ACTGG 1 cut(s) 508
BssMI GATC 3 cut(s) 186, 216, 252
BssNI GRCGYC 4 cut(s) 102, 407, 449, 495
Bst2UI CCWGG 2 cut(s) 208, 463
Bst4CI ACNGT 4 cut(s) 28, 91, 109, 491
BstACI GRCGYC 4 cut(s) 102, 407, 449, 495
BstC8I GCNNGC 1 cut(s) 204
BstDEI CTNAG 2 cut(s) 323, 335
BstF5I GGATG 2 cut(s) 346, 390
BstFNI CGCG 1 cut(s) 419
BstHHI GCGC 2 cut(s) 202, 443
BstKTI GATC 3 cut(s) 189, 219, 255
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 3 cut(s) 186, 216, 252
BstMCI CGRYCG 1 cut(s) 255
BstNI CCWGG 2 cut(s) 208, 463
BstSCI CCNGG 2 cut(s) 206, 461
BstSLI GKGCMC 1 cut(s) 303
BstUI CGCG 1 cut(s) 419
BstV1I GCAGC 1 cut(s) 47
BsuI GTATCC 1 cut(s) 483
BsuRI GGCC 1 cut(s) 163
BtsCI GGATG 2 cut(s) 346, 390
BtsIMutI CAGTG 1 cut(s) 496
Cac8I GCNNGC 1 cut(s) 204
CfoI GCGC 2 cut(s) 202, 443
Cfr13I GGNCC 2 cut(s) 96, 161
CpoI CGGWCCG 1 cut(s) 96
CseI GACGC 2 cut(s) 110, 484
CsiI ACCWGGT 1 cut(s) 461
Csp6I GTAC 2 cut(s) 127, 415
CspI CGGWCCG 1 cut(s) 96
CviAII CATG 1 cut(s) 220
CviJI RGCY 6 cut(s) 38, 163, 206, 309, 334, 528
CviKI_1 RGCY 6 cut(s) 38, 163, 206, 309, 334, 528
CviQI GTAC 2 cut(s) 127, 415
DdeI CTNAG 2 cut(s) 323, 335
DpnI GATC 3 cut(s) 188, 218, 254
DpnII GATC 3 cut(s) 186, 216, 252
DrdI GACNNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 107
EciI GGCGGA 2 cut(s) 152, 171
Eco47I GGWCC 1 cut(s) 96
Eco88I CYCGRG 2 cut(s) 302, 534
EcoRII CCWGG 2 cut(s) 206, 461
Esp3I CGTCTC 1 cut(s) 134
FaeI CATG 1 cut(s) 223
FaiI YATR 4 cut(s) 15, 17, 221, 368
FatI CATG 1 cut(s) 219
FauI CCCGC 1 cut(s) 284
FbaI TGATCA 1 cut(s) 216
FblI GTMKAC 1 cut(s) 410
Fnu4HI GCNGC 2 cut(s) 36, 261
FokI GGATG 2 cut(s) 353, 397
Fsp4HI GCNGC 2 cut(s) 36, 261
FspI TGCGCA 1 cut(s) 201
GlaI GCGC 2 cut(s) 201, 442
GluI GCNGC 2 cut(s) 36, 261
HaeIII GGCC 1 cut(s) 163
HapII CCGG 2 cut(s) 94, 130
HgaI GACGC 2 cut(s) 110, 484
HhaI GCGC 2 cut(s) 202, 443
Hin1I GRCGYC 4 cut(s) 102, 407, 449, 495
Hin1II CATG 1 cut(s) 223
Hin6I GCGC 2 cut(s) 200, 441
HinP1I GCGC 2 cut(s) 200, 441
HincII GTYRAC 2 cut(s) 276, 411
HindII GTYRAC 2 cut(s) 276, 411
HinfI GANTC 2 cut(s) 146, 211
HpaII CCGG 2 cut(s) 94, 130
HphI GGTGA 2 cut(s) 103, 391
Hpy166II GTNNAC 3 cut(s) 276, 394, 411
Hpy188I TCNGA 4 cut(s) 151, 216, 257, 324
Hpy188III TCNNGA 3 cut(s) 94, 355, 534
Hpy8I GTNNAC 3 cut(s) 276, 394, 411
Hpy99I CGWCG 8 cut(s) 104, 409, 412, 415, 451, 454, 457, 500
HpyAV CCTTC 4 cut(s) 91, 146, 382, 392
HpyCH4III ACNGT 4 cut(s) 28, 91, 109, 491
HpyCH4IV ACGT 3 cut(s) 407, 413, 449
HpyCH4V TGCA 2 cut(s) 229, 316
HpyF3I CTNAG 2 cut(s) 323, 335
HpySE526I ACGT 3 cut(s) 407, 413, 449
Hsp92I GRCGYC 4 cut(s) 102, 407, 449, 495
Hsp92II CATG 1 cut(s) 223
HspAI GCGC 2 cut(s) 200, 441
Kpn2I TCCGGA 1 cut(s) 93
Ksp22I TGATCA 1 cut(s) 216
Kzo9I GATC 3 cut(s) 186, 216, 252
LmnI GCTCC 1 cut(s) 257
Lsp1109I GCAGC 1 cut(s) 47
MabI ACCWGGT 1 cut(s) 461
MaeII ACGT 3 cut(s) 407, 413, 449
MaeIII GTNAC 4 cut(s) 85, 109, 326, 499
MalI GATC 3 cut(s) 188, 218, 254
MbiI CCGCTC 1 cut(s) 260
MboI GATC 3 cut(s) 186, 216, 252
MboII GAAGA 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 303
MluCI AATT 1 cut(s) 517
MlyI GAGTC 1 cut(s) 140
MnlI CCTC 9 cut(s) 56, 127, 152, 184, 240, 330, 341, 375, 414
MroI TCCGGA 1 cut(s) 93
MslI CAYNNNNRTG 1 cut(s) 224
MspA1I CMGCKG 2 cut(s) 479, 514
MspI CCGG 2 cut(s) 94, 130
MspR9I CCNGG 2 cut(s) 208, 463
MvaI CCWGG 2 cut(s) 208, 463
MvnI CGCG 1 cut(s) 419
NdeII GATC 3 cut(s) 186, 216, 252
NlaIII CATG 1 cut(s) 223
NlaIV GGNNCC 3 cut(s) 195, 300, 510
NmuCI GTSAC 2 cut(s) 85, 109
NsbI TGCGCA 1 cut(s) 201
PaeR7I CTCGAG 1 cut(s) 534
PcsI WCGNNNNNNNCGW 1 cut(s) 416
PfeI GAWTC 1 cut(s) 211
Pfl23II CGTACG 1 cut(s) 414
PflFI GACNNNGTC 1 cut(s) 451
PkrI GCNGC 2 cut(s) 37, 262
Ple19I CGATCG 1 cut(s) 255
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
Psp6I CCWGG 2 cut(s) 206, 461
PspGI CCWGG 2 cut(s) 206, 461
PspLI CGTACG 1 cut(s) 414
PspN4I GGNNCC 3 cut(s) 195, 300, 510
PspPI GGNCC 2 cut(s) 96, 161
PsrI GAACNNNNNNTAC 2 cut(s) 492, 524
PsyI GACNNNGTC 1 cut(s) 451
PvuI CGATCG 1 cut(s) 255
RsaI GTAC 2 cut(s) 128, 416
RsaNI GTAC 2 cut(s) 127, 415
RseI CAYNNNNRTG 1 cut(s) 224
Rsr2I CGGWCCG 1 cut(s) 96
RsrII CGGWCCG 1 cut(s) 96
SalI GTCGAC 1 cut(s) 409
SatI GCNGC 2 cut(s) 36, 261
Sau3AI GATC 3 cut(s) 186, 216, 252
Sau96I GGNCC 2 cut(s) 96, 161
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 2 cut(s) 208, 463
SduI GDGCHC 1 cut(s) 303
SexAI ACCWGGT 1 cut(s) 461
Sfr274I CTCGAG 1 cut(s) 534
SgrDI CGTCGACG 1 cut(s) 409
SinI GGWCC 1 cut(s) 96
SlaI CTCGAG 1 cut(s) 534
SmiMI CAYNNNNRTG 1 cut(s) 224
SmlI CTYRAG 1 cut(s) 534
SmoI CTYRAG 1 cut(s) 534
Sse9I AATT 1 cut(s) 517
SsiI CCGC 7 cut(s) 114, 137, 156, 260, 291, 479, 512
SspI AATATT 1 cut(s) 545
StyD4I CCNGG 2 cut(s) 206, 461
TaaI ACNGT 4 cut(s) 28, 91, 109, 491
TaiI ACGT 3 cut(s) 410, 416, 452
TaqI TCGA 5 cut(s) 10, 189, 410, 431, 535
TaqII GACCGA 1 cut(s) 113
TasI AATT 1 cut(s) 517
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 1 cut(s) 211
TscAI CASTG 1 cut(s) 496
TseFI GTSAC 2 cut(s) 85, 109
TseI GCWGC 1 cut(s) 35
Tsp45I GTSAC 2 cut(s) 85, 109
TspDTI ATGAA 3 cut(s) 274, 383, 401
TspGWI ACGGA 3 cut(s) 158, 261, 293
TspRI CASTG 1 cut(s) 496
Tth111I GACNNNGTC 1 cut(s) 451
VpaK11BI GGWCC 1 cut(s) 96
XhoI CTCGAG 1 cut(s) 534
XmiI GTMKAC 1 cut(s) 410
ZraI GACGTC 2 cut(s) 408, 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.