MD07G1126400.v1.1

BON1-associated protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002449
Physical Location & Seq
Forward (+)
17836471 .. 17837007
537 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1126400.v1.1.491

Sequence Viewer

Length: 537 bp
ATGGCAGCCAATTCCCGGACGCTCGAAATCAAGGTGATCTCCGCTGAGAATCTGCAATTAGATAGAAAACCCATCAAGAAAAACGCTACCGTGACCGTCTGGACTGACGGCAACAGCCAGTTTTGCTCCACGGACATGGACATCGATGGGGGCGCCTACCCACGGTGGAACGAGAAGCTCGTGCTCAACTTCCCAACGCACTCCAAGTCCATCACGGTGGAGGTCCAATGCAAGACTTCGTCCGGCATCAGGACGATCGGGACGGCGACGATTCCGGAGTCGGATTTTGTTGGCGGGTACGTGCCAGAGGGTTGCATGCATTTTTTGAGTTACAGGCTGAGAGATCACAAGGGGGAGAGGAATGGGATTATTAATATTTCTGTGAGGATGAACGTTCATGAGATGTACGCTTGTGCAAGTTCAACGACGTGGTCTCACTCGCAGATGGGGTTTCCGGCGGATGGGAACAAAAGTTTTGGCGGCGGCGTTGTAACCGGAGTTCCGGTTTGGTGTGGTGCCTATCAGAAAAATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

179

Amino Acids

19.59

Weight (kDa)

8.27

Isoelectric Point (pI)

36.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 7 - 101 1.1e-08 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 152, 515
AccIII TCCGGA 1 cut(s) 274
AciI CCGC 5 cut(s) 42, 294, 458, 480, 483
AclI AACGTT 1 cut(s) 393
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 2 cut(s) 299, 407
AfiI CCNNNNNNNGG 4 cut(s) 15, 162, 249, 461
AgsI TTSAA 1 cut(s) 423
AjiI CACGTC 1 cut(s) 429
AluBI AGCT 1 cut(s) 178
AluI AGCT 1 cut(s) 178
Alw21I GWGCWC 1 cut(s) 186
Alw26I GTCTC 1 cut(s) 438
Aor13HI TCCGGA 1 cut(s) 274
ApeKI GCWGC 1 cut(s) 5
AseI ATTAAT 1 cut(s) 372
AspLEI GCGC 1 cut(s) 155
AspS9I GGNCC 1 cut(s) 223
AsuC2I CCSGG 1 cut(s) 16
AsuHPI GGTGA 1 cut(s) 46
AvaII GGWCC 1 cut(s) 223
BanI GGYRCC 2 cut(s) 152, 515
BauI CACGAG 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 186
BbvI GCAGC 1 cut(s) 17
BccI CCATC 5 cut(s) 80, 140, 218, 439, 455
BceAI ACGGC 2 cut(s) 124, 279
BcnI CCSGG 1 cut(s) 16
BcoDI GTCTC 1 cut(s) 438
BfoI RGCGCY 1 cut(s) 156
BisI GCNGC 3 cut(s) 6, 481, 484
BlsI GCNGC 3 cut(s) 7, 482, 485
Bme1390I CCNGG 1 cut(s) 16
Bme18I GGWCC 1 cut(s) 223
BmgBI CACGTC 1 cut(s) 429
BmgT120I GGNCC 1 cut(s) 223
BmiI GGNNCC 2 cut(s) 154, 517
BmrFI CCNGG 1 cut(s) 16
BmsI GCATC 1 cut(s) 255
BpuMI CCSGG 1 cut(s) 16
Bsa29I ATCGAT 1 cut(s) 144
BsaAI YACGTR 1 cut(s) 301
BsaHI GRCGYC 1 cut(s) 153
BsaI GGTCTC 1 cut(s) 438
BsaJI CCNNGG 2 cut(s) 129, 161
BsaWI WCCGGW 3 cut(s) 274, 494, 502
BsaXI ACNNNNNCTCC 2 cut(s) 489, 519
Bsc4I CCNNNNNNNGG 4 cut(s) 15, 162, 249, 461
Bse1I ACTGG 1 cut(s) 118
BseAI TCCGGA 1 cut(s) 274
BseCI ATCGAT 1 cut(s) 144
BseDI CCNNGG 2 cut(s) 129, 161
BseGI GGATG 2 cut(s) 393, 466
BseLI CCNNNNNNNGG 4 cut(s) 15, 162, 249, 461
BseMII CTCAG 2 cut(s) 36, 329
BseNI ACTGG 1 cut(s) 118
BseXI GCAGC 1 cut(s) 17
Bsh1285I CGRYCG 1 cut(s) 258
BshNI GGYRCC 2 cut(s) 152, 515
BshVI ATCGAT 1 cut(s) 144
BsiEI CGRYCG 1 cut(s) 258
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 6 cut(s) 16, 243, 275, 455, 495, 503
BslFI GGGAC 1 cut(s) 274
BslI CCNNNNNNNGG 4 cut(s) 15, 162, 249, 461
BsmAI GTCTC 1 cut(s) 438
BsmFI GGGAC 1 cut(s) 274
Bso31I GGTCTC 1 cut(s) 438
Bsp1286I GDGCHC 1 cut(s) 186
Bsp13I TCCGGA 1 cut(s) 274
Bsp143I GATC 3 cut(s) 36, 255, 343
BspACI CCGC 5 cut(s) 42, 294, 458, 480, 483
BspCNI CTCAG 2 cut(s) 37, 330
BspDI ATCGAT 1 cut(s) 144
BspEI TCCGGA 1 cut(s) 274
BspHI TCATGA 1 cut(s) 397
BspLI GGNNCC 2 cut(s) 154, 517
BspT107I GGYRCC 2 cut(s) 152, 515
BspTNI GGTCTC 1 cut(s) 438
BsrI ACTGG 1 cut(s) 118
BssECI CCNNGG 2 cut(s) 129, 161
BssMI GATC 3 cut(s) 36, 255, 343
BssNI GRCGYC 1 cut(s) 153
BssSI CACGAG 1 cut(s) 179
Bst2BI CACGAG 1 cut(s) 179
Bst4CI ACNGT 4 cut(s) 91, 97, 165, 217
BstACI GRCGYC 1 cut(s) 153
BstBAI YACGTR 1 cut(s) 301
BstC8I GCNNGC 1 cut(s) 317
BstDEI CTNAG 2 cut(s) 45, 338
BstDSI CCRYGG 2 cut(s) 129, 161
BstF5I GGATG 2 cut(s) 393, 466
BstH2I RGCGCY 1 cut(s) 156
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 3 cut(s) 39, 258, 346
BstMAI GTCTC 1 cut(s) 438
BstMBI GATC 3 cut(s) 36, 255, 343
BstMCI CGRYCG 1 cut(s) 258
BstMWI GCNNNNNNNGC 1 cut(s) 123
BstNSI RCATGY 1 cut(s) 319
BstSCI CCNGG 1 cut(s) 14
BstV1I GCAGC 1 cut(s) 17
BstXI CCANNNNNNTGG 2 cut(s) 136, 217
Bsu15I ATCGAT 1 cut(s) 144
BsuTUI ATCGAT 1 cut(s) 144
BtgI CCRYGG 2 cut(s) 129, 161
BtrI CACGTC 1 cut(s) 429
BtsCI GGATG 2 cut(s) 393, 466
Cac8I GCNNGC 1 cut(s) 317
CciI TCATGA 1 cut(s) 397
CfoI GCGC 1 cut(s) 155
Cfr13I GGNCC 1 cut(s) 223
ClaI ATCGAT 1 cut(s) 144
CseI GACGC 1 cut(s) 28
Csp6I GTAC 2 cut(s) 298, 406
CviAII CATG 3 cut(s) 136, 316, 398
CviJI RGCY 4 cut(s) 8, 117, 178, 337
CviKI_1 RGCY 4 cut(s) 8, 117, 178, 337
CviQI GTAC 2 cut(s) 298, 406
DdeI CTNAG 2 cut(s) 45, 338
DinI GGCGCC 1 cut(s) 154
DpnI GATC 3 cut(s) 38, 257, 345
DpnII GATC 3 cut(s) 36, 255, 343
EciI GGCGGA 1 cut(s) 473
Eco31I GGTCTC 1 cut(s) 438
Eco47I GGWCC 1 cut(s) 223
EcoT22I ATGCAT 1 cut(s) 321
EgeI GGCGCC 1 cut(s) 154
EheI GGCGCC 1 cut(s) 154
FaeI CATG 3 cut(s) 139, 319, 401
FaiI YATR 3 cut(s) 137, 317, 399
FaqI GGGAC 1 cut(s) 274
FatI CATG 3 cut(s) 135, 315, 397
FauI CCCGC 1 cut(s) 287
Fnu4HI GCNGC 3 cut(s) 6, 481, 484
FokI GGATG 2 cut(s) 400, 473
Fsp4HI GCNGC 3 cut(s) 6, 481, 484
GlaI GCGC 1 cut(s) 154
GluI GCNGC 3 cut(s) 6, 481, 484
HaeII RGCGCY 1 cut(s) 156
HapII CCGG 6 cut(s) 16, 243, 275, 455, 495, 503
HgaI GACGC 1 cut(s) 28
HhaI GCGC 1 cut(s) 155
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 3 cut(s) 139, 319, 401
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HinfI GANTC 3 cut(s) 49, 271, 278
HpaII CCGG 6 cut(s) 16, 243, 275, 455, 495, 503
HphI GGTGA 1 cut(s) 46
Hpy188I TCNGA 2 cut(s) 283, 525
Hpy188III TCNNGA 6 cut(s) 76, 100, 250, 259, 275, 398
Hpy99I CGWCG 2 cut(s) 271, 430
HpyCH4III ACNGT 4 cut(s) 91, 97, 165, 217
HpyCH4IV ACGT 3 cut(s) 300, 393, 428
HpyCH4V TGCA 5 cut(s) 55, 231, 315, 319, 416
HpyF10VI GCNNNNNNNGC 1 cut(s) 123
HpyF3I CTNAG 2 cut(s) 45, 338
HpySE526I ACGT 3 cut(s) 300, 393, 428
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 3 cut(s) 139, 319, 401
HspAI GCGC 1 cut(s) 153
KasI GGCGCC 1 cut(s) 152
Kpn2I TCCGGA 1 cut(s) 274
Kzo9I GATC 3 cut(s) 36, 255, 343
LmnI GCTCC 1 cut(s) 131
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 255
MaeII ACGT 3 cut(s) 300, 393, 428
MaeIII GTNAC 3 cut(s) 91, 329, 490
MalI GATC 3 cut(s) 38, 257, 345
MboI GATC 3 cut(s) 36, 255, 343
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 3 cut(s) 10, 56, 529
Mly113I GGCGCC 1 cut(s) 153
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 1 cut(s) 261
MnlI CCTC 4 cut(s) 214, 301, 351, 378
Mph1103I ATGCAT 1 cut(s) 321
MroI TCCGGA 1 cut(s) 274
MseI TTAA 1 cut(s) 372
MslI CAYNNNNRTG 2 cut(s) 134, 215
MspA1I CMGCKG 1 cut(s) 44
MspI CCGG 6 cut(s) 16, 243, 275, 455, 495, 503
MspR9I CCNGG 1 cut(s) 16
MwoI GCNNNNNNNGC 1 cut(s) 123
NarI GGCGCC 1 cut(s) 153
NciI CCSGG 1 cut(s) 16
NdeII GATC 3 cut(s) 36, 255, 343
NlaIII CATG 3 cut(s) 139, 319, 401
NlaIV GGNNCC 2 cut(s) 154, 517
NmuCI GTSAC 1 cut(s) 91
NsiI ATGCAT 1 cut(s) 321
NspI RCATGY 1 cut(s) 319
PaeI GCATGC 1 cut(s) 319
PagI TCATGA 1 cut(s) 397
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PfeI GAWTC 2 cut(s) 49, 271
PflFI GACNNNGTC 2 cut(s) 238, 430
PfoI TCCNGGA 1 cut(s) 14
PkrI GCNGC 3 cut(s) 7, 482, 485
Ple19I CGATCG 1 cut(s) 258
PleI GAGTC 1 cut(s) 286
PluTI GGCGCC 1 cut(s) 156
PpsI GAGTC 1 cut(s) 286
Ppu21I YACGTR 1 cut(s) 301
PshBI ATTAAT 1 cut(s) 372
Psp1406I AACGTT 1 cut(s) 393
PspN4I GGNNCC 2 cut(s) 154, 517
PspPI GGNCC 1 cut(s) 223
PsrI GAACNNNNNNTAC 2 cut(s) 483, 515
PsyI GACNNNGTC 2 cut(s) 238, 430
PvuI CGATCG 1 cut(s) 258
RsaI GTAC 2 cut(s) 299, 407
RsaNI GTAC 2 cut(s) 298, 406
RseI CAYNNNNRTG 2 cut(s) 134, 215
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 3 cut(s) 6, 481, 484
Sau3AI GATC 3 cut(s) 36, 255, 343
Sau96I GGNCC 1 cut(s) 223
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 1 cut(s) 16
SduI GDGCHC 1 cut(s) 186
SetI ASST 6 cut(s) 36, 180, 225, 303, 396, 431
SfaNI GCATC 1 cut(s) 255
SfoI GGCGCC 1 cut(s) 154
SinI GGWCC 1 cut(s) 223
SmiMI CAYNNNNRTG 2 cut(s) 134, 215
SphI GCATGC 1 cut(s) 319
Sse9I AATT 3 cut(s) 10, 56, 529
SsiI CCGC 5 cut(s) 42, 294, 458, 480, 483
SspDI GGCGCC 1 cut(s) 152
SspI AATATT 1 cut(s) 376
StyD4I CCNGG 1 cut(s) 14
TaaI ACNGT 4 cut(s) 91, 97, 165, 217
TaiI ACGT 3 cut(s) 303, 396, 431
TaqI TCGA 2 cut(s) 24, 144
TasI AATT 3 cut(s) 10, 56, 529
TauI GCSGC 2 cut(s) 483, 486
TfiI GAWTC 2 cut(s) 49, 271
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 2 cut(s) 386, 404
TspGWI ACGGA 1 cut(s) 146
Tth111I GACNNNGTC 2 cut(s) 238, 430
VpaK11BI GGWCC 1 cut(s) 223
VspI ATTAAT 1 cut(s) 372
XceI RCATGY 1 cut(s) 319
Zsp2I ATGCAT 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.